New software features of the 7010 Particle Size. Analyzer Danielle Chamberlin May May 5, Page 1

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1 New software features of the 7010 Particle Size May 5, 2009 Analyzer Danielle Chamberlin May Page 1

2 Introduction to the new 7010 software Instrument operation New look and feel easy to operate document format Data display options Advanced features Restricting wavelength/particle size range - notch Particle fit algorithm choices Time Series Stitch The materials library and editing RI Reporting Licensing Page 2

3 Installation 1. Find Agilent 82357B USB-GPIB cable 2. Plug in GPIB end to instrument 3. Plug USB end into computer Page 3

4 Power on instrument Page 4

5 Instrument operation The 7010 software now directly controls the 7010 instrument. There is no need to run Chemstation in the background. Three simple buttons are all that s t required to make a measurement. Page 5

6 Turning the lamps on Lamp icon When the software is first started, the lightbulb icon turns on. The warming state will display. The lamps must warm for 10 minutes before measurement is enabled. This ensures the lamps are stable and measurements are valid. Page 6

7 Blank and sample measurement blank sample After the 10-minute warmup period, the lamp icon changes to a fully lit state. t The blank icon (cuvette with liquid id but no particles) will now become enabled. After a blank is measured, the sample icon (cuvette with liquid and particles in it) will become enabled. Page 7

8 Making a blank measurement 1. Fill cuvette with water or suspending fluid 2. Insert into sample holder 3. Click blank icon in 7010 software Page 8

9 Blank measurement When a blank is measured, a window will pop up displaying i the difference between two successive blank measurements. If the lamps are stable, this noise measurement should be low (<0.001 AU). This popup may be clicked away after review. Page 9

10 Making particle size measurements When the measure icon is clicked, a spectrum is measured from the 7010 hardware. The particle type, fluid, and pathlength must be selected for the software to calculate particle size. Page 10

11 Signal saturation The new 7010 software warns the user when they have taken a bad measurement that saturates the detector. The user is advised that they may wish to dilute or shorten the pathlength. Page 11

12 Graphical User Interface The 7010 PSA software uses a document format to better match the user s workflow. Rather than one sample per file a logical set of measurements can be combined together in one file for easy review. Page 12

13 Editing measurement names When a measurement is made, a time and date stamp will appear as the ID for that measurement in the spectrum list view. Clicking once will select that spectrum to be viewed in the spectrum view and particle size view. Clicking a second time on the time/date stamp will allow the user to edit the spectrum ID to a more descriptive name. Page 13

14 Measurement comments Additional annotations about a particular measurement may be made by right clicking on the spectrum name in the spectrum list view and selecting Properties. Page 14

15 Comparing spectra and PSD measurements Simply shift-click or control-click on multiple spectrum names to compare them. Page 15

16 Drag and drop Spectra can be dragged d and dropped d between documents, or dragged d within a document to re-order. Spectra can also be copied and pasted within or between documents, and deleted, by menu commands or the familiar Ctrl-C, Ctrl-V, Ctrl-X (or Del) key commands. Page 16

17 Drag and Drop Page 17

18 Data display options Data can be displayed in concentration vs size, cumulative concentration vs size, or both overlaid. It can be displayed as volume density or number density. Page 18

19 Mean particle size view The particle size view can also display the mean particle size of all spectra in the document (or all selected spectra) as a bar chart. This is especially useful in a time series measurement. Page 19

20 Restricting wavelength range Due to absorbing molecules in the fluid, it is sometimes necessary to remove certain wavelengths from the particle size calculation. This can be done through the menu bar under Data or by right-clicking g anywhere in the spectrum view window. Page 20

21 Restricting wavelength range (2) When the wavelength range is restricted, sliders appear at the top of the spectrum view which can be moved to further adjust the wavelength range. De-selected wavelengths appear grey on the screen. Page 21

22 Notch If you would like to remove wavelengths in the middle of the spectrum, use the notch feature. Page 22

23 Choice of algorithms to fit data UV Autocorrect Often there are UV-absorbing molecules in the fluid phase which are not correctly modeled in the fit. By default, the 7010 algorithm ignores these wavelengths if doing so improves the fit. If there are small particles in your sample, you may need to turn this feature off. Page 23

24 Calculation options window Deselect the checkbox marked Automatically Adjust for Poor UV Fit and click OK. Page 24

25 UV Autocorrect Now the population p of 100 nm particles in the sample shows up. You may wish to make a copy of the measurement before altering calculation options so you can easily see the effect. Page 25

26 Calculation options The 7010 particle size fitting algorithms use a variety of proprietary approaches to fit the measured data. In default mode, the computer automatically reports the calculation with the best fit to the measured spectrum. If the user wishes to force the particle size distribution to be monodisperse, fit a Gaussian profile, or expects the distribution to be broad, they may force the algorithm to use this fit in the calculation options window. Page 26

27 Time Series The fast data acquisition time of the 7010 Particle Size Analyzer allows measurements of fast-changing samples not possible with other particle size measurement techniques. To acquire a time series, first, measure a blank. Then insert your sample. Select Instrument -> Time Series. Type the total run time desired, the number of samples, and linear or log spacing between measurements in time. Page 27

28 Stitch For most measurements the high dynamic range of the 7010 is sufficient. For measurements which contain extremely high attenuations along with extremely low attenuations where it is important to accurately measure both, the Stitch feature allows you to take measurements with two different path length cuvettes and combine them. Simply select two measurements of the same sample with different pathlengths, and go to Data->Advanced->Stitch. Click the cursor (vertical red line) at the wavelength where you wish to stitch the two spectra together. The longer pathlength sample will be used to the right of the red line, the shorter pathlength to the left. Page 28

29 The Materials Library The 7010 Particle Size Analyzer has a large list of materials to choose from in its materials library. To see the real and imaginary refractive indices used in the particle size calculations, go to Data -> Advanced -> Materials Library. Page 29

30 Adding and editing materials The materials library allows you to edit existing refractive index files and add your own. For example, you may copy an existing RI file to one with a new name, then edit the new RI file. Page 30

31 Editing RI You may edit the RI by simply clicking in the data spreadsheet and typing in new values. Once the values have been changed, the floppy disk Save icon becomes orange and will stay highlighted hli h until your new material is saved. Page 31

32 Making a new material You can enter an entirely new refractive index into your materials library. To do this, click on the gold plus icon, and enter the new material s name. The refractive index numbers can be typed in directly, or simply be cut and pasted from three columns in an Excel spreadsheet containing wavelength, n, and k. Page 32

33 Measuring k If the particle phase of your sample can be made into a fluid, for example if your sample is an emulsion or particles in your dispersion can be dissolved, k may be measured directly by the absorbance measurement on the Deselect any particle or fluid selections, take a blank (either of water if your sample is a fluid, or of the solvent if your sample is dissolved). The resulting spectral attenuation measurement can be exported under File-> Export Selected Spectrum Data. The imaginary RI for a pure fluid can be calculated from the absorption measurement as Aλ k = where A is absorption and l is the pathlength. 4πl Aλ For a solution the k of the solute is k = where C v is the volume concentration. 4πlCv Page 33

34 Transparent materials The refractive index of transparent materials is well-modeled by the Sellmeier equation. If no k data is entered, the user has a choice to have the software use raw n data or perform a Sellmeier fit to the data. If a Sellmeier fit is performed, the wavelength range will be extended to 190 nm and 1100 nm even if the entered data does not extend over this range. Page 34

35 Sharing custom RI files If you make a custom RI file and wish to share your particle analysis result with another customer, you will need to also share the refractive index file. The import and export icons in the Materials Library allow you to share these.rfi ffiles. Page 35

36 Reporting The software can generate e e a report of the measured ed data. a To access reporting features, go to File-> Generate Report or simply press Ctrl-P. Page 36

37 Licensing Your 7010 software may be copied onto as many computers as you wish without control of an instrument, simply for data analysis. For instrument control, a valid license key must be present. To obtain the license key, you will need your license entitlement certificate, shipped with your 7010 instrument. It should look something like the picture at right. Page 37

38 Adding a license To obtain the license, go to the Agilent Licensing Website either by clicking on Help-> License Redemption Website from within the 7010 software, or by navigating directly to Follow the instructions on the website. You will need your Software Entitlement Certificate as well as your Host ID as reported by the Agilent License Notifier: The Host ID will appear when you hover your mouse cursor over the blue key icon in your system tray. After completing the steps on the website, you will receive a.lic file via . Save the.lic file to the computer being licensed. From within the 7010 software, select "Install License(s)..." under the Help menu. Browse to your saved.lic file. You should see a bubble in the system tray that says that the license was installed successfully. Page 38

39 Support The hardware and software manuals are provided as.pdf documents with your 7010 software CD. For additional support, in North America call (800) x3. Outside North America, contact your local distributor. Page 39

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