CHROMATOGRAPHY CHROMATOGRAPHY. A World of Expertise, in your hands

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1 A World of Expertise, in your hands

2 Chromaflex, Flex-Column and Disposaflex Chromatography Columns - (pages 2-7) TLC Tanks, Sprayers & Accessories (pages 8-2) Ultra-Ware HPLC Mobile Phase Handling Systems - (pages 22-28) Chromatography Vials and Accessories (pages 29-0) CHROMAFLEX Columns CHROMAFLEX Chromatography Columns have a unique design feature: PTFE shielded o-rings. Each end fitting has a thin PTFE layer that is curled over the o- ring to form a solvent resistant, leak free seal. CHROMAFLEX Columns can be used with all aqueous buffers without modification and with organic solvents when the optional PTFE bed supports are installed in the end fittings and flow adapters. CHROMAFLEX Columns are available in both standard and water jacketed versions. Standard CHROMAFLEX Columns Standard CHROMAFLEX Columns are available in three IDs (.0, 2.5 and 4.8 cm) in five standard lengths (5, 0, 60, 00 and 20 cm). Each column is supplied with two PTFE end fittings with 20 µm porosity polyethylene bed supports, 5 feet PTFE tubing (/6 OD tubing with.0 cm, /8 OD tubing with 2.5 & 4.8 cm), 2 flangeless tubing nuts and 2 flangeless ferrules. Jacketed CHROMAFLEX Columns Jacketed CHROMAFLEX Columns are provided with an acrylic water jacket to provide thermal control of temperature sensitive chromatographic separations. The acrylic jacket also acts as a safety shield. Jacketed columns are available in all of the standard CHROMAFLEX Column sizes and supplied with the same tubing, nuts and ferrules. Flow Adapters CHROMAFLEX Flow Adapters allow easy adjustment of the column bed volume. They are ideal for concentration and ph gradients, reverse flow separations, and gravity flow separations that require a high level of resolution and reproducibility. Flow adapters are available for all three standard ID columns. Packing Reservoirs The CHROMAFLEX Glass Reservoirs are ideal for packing gel slurries into columns and as buffer reservoirs for simple gravity flow chromatography. Each reservoir has been designed for easy mounting on all CHROMAFLEX Columns. The reservoir capacities are matched by column ID to take the amount of slurry required to pack the longest standard column. The reservoir end fitting has /4-28 threads for simple flangeless connection to a pump. Flangeless /4-28 Fittings All tubing connections to CHROMAFLEX Columns can be made with easy-to-use /4-28 flangeless fittings and either /6 OD, /8 OD or.8 mm OD tubing. Our complete line of fittings and adapters allow simple connection to any chromatography system. Custom Length Columns Certain applications may require a column length not found in our standard product offering. Please contact customer service for pricing and delivery details for custom length CHROMAFLEX Columns. Specifications jacketed column shown with optional flow adapter. Materials Column Barrels: End Fitting: Bed Support: Water/Safety Jacket: Jacket Sealing Rings: Type I, Class A Borosilicate Glass Polytetrafluoroethylene (PTFE) High Density Polyethylene (HDPE) Acrylic (jacketed columns only) Polypropylene (jacketed columns only) Bed Support Porosity: Maximum Pressure*: 20 micron nominal (for standard bed support).0 cm ID Columns - 00 psi (6.9 bar) 2.5 cm ID Columns 75 psi (5.2 bar) 4.8 cm ID Columns - 50 psi (.4 bar) Temperature Range: 0 to 50 C Tubing Connections: /4-28 flangeless fittings Recommended: Sterilization Method: Ethylene Oxide, 2N NaOH or 00% Ethanol (autoclaving is not recommended)

3 CHROMAFLEX Standard Threaded Columns Columns are supplied complete with glass barrel and 2 end fittings with 20 µm porosity HDPE bed supports. Each column is also supplied with 5 feet (.5 m) of FEP tubing (/6 OD with.0 cm; /8 OD with 2.5 and 4.8 cm), 2 flangeless tubing nuts and 2 flangeless ferrules. Catalog Column ID Length Volume Max. Pressure* Number (cm) (cm) (ml) (psi/bar) Qty / / / / / / / / / / / / / / /.4 Replacement Parts Glass Barrel Only,.0 x 5 cm Glass Barrel Only,.0 x 0 cm Glass Barrel Only,.0 x 60 cm Glass Barrel Only,.0 x 00 cm Glass Barrel Only,.0 x 20 cm Glass Barrel Only, 2.5 x 5 cm Glass Barrel Only, 2.5 x 0 cm Glass Barrel Only, 2.5 x 60 cm Glass Barrel Only, 2.5 x 00 cm Glass Barrel Only, 2.5 x 20 cm Glass Barrel Only, 4.8 x 5 cm Glass Barrel Only, 4.8 x 0 cm Glass Barrel Only, 4.8 x 60 cm Glass Barrel Only, 4.8 x 00 cm Glass Barrel Only, 4.8 x 20 cm cm End Fitting w/ Bed Support cm End Fitting w/ Bed Support cm End Fitting w/ Bed Support Bed Support, HDPE,.0 cm, 20 µm Bed Support, HDPE, 2.5 cm, 20 µm Bed Support, HDPE, 4.8 cm, 20 µm Screw Cap,.0 cm Screw Cap, 2.5 cm Screw Cap, 4.8 cm Accessories Bed Support, PTFE,.0 cm, 20 µm Bed Support, PTFE, 2.5 cm, 20 µm Bed Support, PTFE, 4.8 cm, 20 µm * CAUTION Pressurized glassware should always be used with adequate safety shielding CHROMAFLEX Standard Column Cross Section heavy wall glass column interchangeable bed supports (HDPE or PTFE) PTFE o-ring shield provides a leak-free seal threaded acetal screw cap PTFE end fitting CTFE /2-20 to /4-28 thread adapter

4 CHROMAFLEX Jacketed Threaded Columns Columns are supplied complete with glass barrel, water jacket and 2 end fittings with 20 µm porosity HDPE bed supports. Each column is also supplied with 5 feet (.5 m) of FEP tubing (/6 OD with.0 cm; /8 OD with 2.5 and 4.8 cm), 2 flangeless tubing nuts and 2 flangeless ferrules. Catalog Column ID Length Volume Max. Pressure* Number (cm) (cm) (ml) (psi/bar) Qty / / / / / / / / / / / / / / /.4 Replacement Parts CHROMAFLEX Jacketed Column Cross Section heavy wall glass column acrylic jacket HDPE or PTFE support PTFE o-ring shield provides a leak-free seal jacket sealing ring Glass Barrel Only,.0 x 5 cm Glass Barrel Only,.0 x 0 cm Glass Barrel Only,.0 x 60 cm Glass Barrel Only,.0 x 00 cm Glass Barrel Only,.0 x 20 cm Glass Barrel Only, 2.5 x 5 cm Glass Barrel Only, 2.5 x 0 cm Glass Barrel Only, 2.5 x 60 cm Glass Barrel Only, 2.5 x 00 cm Glass Barrel Only, 2.5 x 20 cm Glass Barrel Only, 4.8 x 5 cm Glass Barrel Only, 4.8 x 0 cm Glass Barrel Only, 4.8 x 60 cm Glass Barrel Only, 4.8 x 00 cm Glass Barrel Only, 4.8 x 20 cm cm End Fitting w/ Bed Support cm End Fitting w/ Bed Support cm End Fitting w/ Bed Support End Fitting with Bed Support, /6.0 cm End Fitting with Bed Support, /6 2.5 cm End Fitting with Bed Support, /8 2.5 cm End Fitting with Bed Support, /8 4.8 cm Jacket Sealing Ring.0 cm Jacket Sealing Ring 2.5 cm Jacket Sealing Ring 4.8 cm Bed Support, HDPE,.0 cm, 20 µm Bed Support, HDPE, 2.5 cm, 20 µm Bed Support, HDPE, 4.8 cm, 20 µm Screw Cap,.0 cm Screw Cap, 2.5 cm Screw Cap, 4.8 cm Accessories Bed Support, PTFE,.0 cm, 20 µm Bed Support, PTFE, 2.5 cm, 20 µm Bed Support, PTFE, 4.8 cm, 20 µm * CAUTION Pressurized glassware should always be used with adequate safety shielding. threaded acetal screw cap PTFE end fitting CTFE /2-20 to /4-28 thread adapter

5 CHROMAFLEX Flow Adapters Flow Adapters improve chromatogram resolution by delivering the sample and buffer directly to the top of the gel bed. This protects the gel bed from disruption during sample loading and eliminates any dead volume above the gel bed. Flow adapters are recommended when using buffer gradients, during reverse flow applications, and for high resolution gravity flow separations. Each flow adapter is supplied with a 20 µm porosity HDPE bed support, TFE / propylene o-ring, stainless steel adjusting rod, PTFE body and 5 feet (.5 meters) of FEP tubing. Use of aggressive organic solvents requires the accessory PTFE bed supports and FFKM o-rings listed below. For Standard Columns Fits Column Catalog ID Tubing Number (cm) OD Qty / / / /8 For Jacketed Columns Fits Column Catalog ID Tubing Number (cm) OD Qty / / / /8 Flow Adapter Replacement Parts Fits Catalog Colum Number Description ID Qty shown with optional flow adapter Bed Support, HDPE, 20 µm porosity.0 cm Bed Support, HDPE, 20 µm porosity 2.5 cm Bed Support, HDPE, 20 µm porosity 4.8 cm Ferrule, Polypropylene, for /6 OD tubing.0 / Ferrule, ETFE, for /8 OD tubing 2.5 / Tubing, FEP, /6 OD x 0 feet.0 / Tubing, FEP, /8 OD x 0 feet 2.5 / 4.8 Flow Adapter Accessory Parts Fits Catalog Colums Number Description ID Qty Bed Support, PTFE, 20 µm porosity.0 cm Bed Support, PTFE, 20 µm porosity 2.5 cm Bed Support, PTFE, 20 µm porosity 4.8 cm CHROMAFLEX Flow Adapter Cross Section Flow Adapter Cross Section FEP tubing polypropylene or ETFE ferrule TFE / propylene or FFKM o-ring HDPE or PTFE bed support

6 CHROMAFLEX Packing Reservoirs for Standard Columns The CHROMAFLEX Packing Reservoirs are ideal for packing gel slurries into columns. Each packing reservoir is designed for easy mounting on CHROMAFLEX Standard Columns. The capacities are matched to take the amount of gel slurry required to pack the longest column. The packing reservoirs may also be used as a buffer reservoir for gravity flow chromatography. For Standard Columns Only Fits Column Catalog ID Nominal Number (cm) Volume Qty ml ml ml CHROMAFLEX Columns - Column bed height and approximate volume data. Without Adapters With One Adapter With Two Adapters ID (cm) Length (cm) Volume (ml) Bed Height (cm) Volume (ml) Bed Height (cm) Volume (ml) Maximum Pressure (psi) (must use with safety jacket) not rated (no safety jacket avail.)

7 CHROMAFLEX FITTINGS The following tubing nuts and ferrules are required to make the initial tubing connections to the end fittings of Chromaflex Columns. Catalog Number Description Material Qty Tubing Nut, for /6 OD tubing PEEK Tubing Nut, for /8 OD tubing PEEK Ferrule, for /6 OD tubing PEEK Ferrule, for /6 OD tubing Polypropylene Ferrule, for /8 OD tubing PEEK Ferrule, for /8 OD tubing Polypropylene Adapter, M/4-28 Thread to /6 Barb ETFE Adapter, M/4-28 Thread to /8 Barb ETFE Adapter, M/4-28 Thread to /6 Barb ETFE Flangeless Nut, /4-28 Thread, for /6 tubing ETFE Flangeless Nut, /4-28 Thread, for /8 and.8mm OD tubing ETFE Flangeless Nut, M6 Thread, for /6 tubing Acetal Flangeless Nut, M6 Thread, for /8 and.8mm OD tubing Acetal Flangeless Ferrule, for /6 OD tubing ETFE Flangeless Ferrule, for.8 mm OD tubing ETFE Flangeless Ferrule, for /8 OD tubing ETFE Tubing, /4 OD x 0.00 ID x 0 feet FEP Tubing, /6 OD x 0.08 ID x 0 feet FEP Tubing, /8 OD x ID x 0 feet FEP H Tubing, /6 OD x 5 feet, with integral F-Luer fitting Tubing - PTFE 5 Luer - CTFE H Union, F/4-28 to F/4-28 CTFE Union, F/4-28 to F/4-28 ETFE Union, F/4-28 to F-M6 ETFE Union, F-M6 to F-M6 ETFE Union, M/4-28 to M/4-28 CTFE Valve, -way, /4-28 Threads, Valve - ETFE with /6 Flangeless Fittings Fittings - ETFE Valve, -way, /4-28 Threads, Valve - ETFE with /8 Flangeless Fittings Fittings - ETFE Valve, -way, /4-28 Threads, Valve - PEEK with /6 Flangeless Fittings Fittings -PEEK/ ETFE Valve, -way, /4-28 Threads, Valve - PEEK with /8 Flangless Fittings Fittings -PEEK/ ETFE

8 Luer Lock Fitting Threaded Reservoir Cap Borosilicate Glass Column Translucent Reservoir Top Precision Ground Column Ends FLEX-COLUMNS are economical, easy-to-use chromatography columns that are ideal for use with gel filtration, ion exchange, affinity and adsorption media. The columns are constructed of polypropylene reservoirs and column outlets permanently connected to a borosilicate glass barrel. The 20 µm porosity polyethylene bed supports are fixed in the column outlets. Luer lock inlets and outlets on the columns, along with a wide variety of adapters, valves and fittings provide simple and versatile tubing connections. Standard Columns Standard FLEX-COLUMNS are available in 0 different sizes with standard inside diameters of 0.7,.0,.5 and 2.5 cm and lengths ranging from 4 to 20 cm. All columns are fitted with translucent polypropylene reservoir tops, and bed supports that are permanently mounted in the polypropylene column outlet. Short columns are typically used for ion exchange chromatography, sample desalting and semidisposable applications, while the long columns are ideally suited for gel filtration and adsorption chromatography. Flow Adapters FLEX-COLUMN Flow Adapters allow easy adjustment of the column bed volume. They are ideal for concentration and ph gradients, reverse flow separations, and gravity flow separations that require a high level of resolution and reproducibility. Flow adapters are available for.0,.5 and 2.5 cm ID columns. Luer Lock Fittings The male luer lock fittings on all FLEX-COLUMNS along with our complete line of fittings, valves and adapters allow easy connection to any low pressure chromatography system. Custom Length Columns Certain applications may require a column length not found in our standard product offering. Please contact Customer Service for pricing and delivery details for custom length FLEX-COLUMNS. Call Specifications Materials: Column Barrels: Type I, Class A Borosilicate Glass Cap, Reservoir & Column Outlet: Polypropylene Bed Support: High Density Polyethylene (HDPE) Bed Support Porosity: 20 micron nominal Maximum Pressure:.0 bar (4.7 psi,.0 atm, 0. MPa) Temperature Range: 0 to 50 C Tubing Connections: Male Luer Locks Recommended: 2N NaOH or 00% Ethanol Sterilization Method: (Autoclaving is not recommended.) Luer Lock Column Outlet Micron Polyethylene Bed Support Note: Polypropylene end fittings are NOT replaceable on FLEX-COLUMNS. * CAUTION Pressurized glassware should always be used with adequate safety shielding

9 FLEX-COLUMN Economy Columns Catalog ID Length Cross Sectional Volume Number (cm) (cm) Area (cm ) (ml) Qty Replacement Column Outlet Cover Catalog Number Qty Catalog ID Length Cross Sectional Volume Number (cm) (cm) Area (cm ) (ml) Qty

10 FLEX-COLUMN Flow Adapters Flow adapters can significantly improve FLEX-COLUMN performance by protecting the gel bed from disruption during sample loading and by eliminating any dead volume above the gel bed. Flow adapters improve chromatogram resolution by delivering the sample and buffer directly to the top of the gel bed. Flow adapters are also recommended when using buffer gradients or when the columns are connected to a low pressure pump system. Flow adapters consist of /6 OD FEP tubing, a polyacetal body, an FKM o-ring seal and a 20 µm porosity HDPE bed support. One replacement bed support is supplied with each flow adapter. Catalog Fits Column Functional Number Description ID (cm) Length (cm) Qty Flow Adapter Flow Adapter Flow Adapter Replacement Parts Column with Flow Adapter Bed Support,.0 cm Bed Support,.5 cm Bed Support, 2.5 cm Ferrule, Polypropylene, /6 OD Tubing, FEP, /6 OD x 0.08 ID 0 feet FLEX-COLUMN Sample Diffusion Discs Sample diffusion discs protect the gel bed from being disrupted during sample application whenever a flow adapter is not used. A transparent acrylic ring is sealed to a 20 µm porosity HDPE disc which protects the top of the gel bed when the sample is manually applied. A cross bar on the top of the acrylic ring permits easy removal of the sample diffusion disc from the column. Catalog Number Description Qty Sample Diffusion Disc,.0 cm Sample Diffusion Disc,.5 cm Sample Diffusion Disc, 2.5 cm Column with Diffusion Disc FLEX-COLUMN Polypropylene Packing Reservoirs The funnels are manufactured from polypropylene and are available in two capacities: 00 ml for the 0.7,.0 and.5 cm ID columns and 700 ml for the 2.5 cm columns. The funnels are used for initial column packing and as a small buffer reservoir. Catalog Capacity Number (ml) Qty

11 FLEX-COLUMN Fittings Catalog Number Description Material Qty. Price Barb for /6 I.D. tubing to female luer polypropylene Barb for /6 I.D. tubing to male luer polypropylene Female luer to female luer polypropylene Female luer to /4-28 thread polypropylene Female luer to female /4-28 thread ETFE Male luer to male /4-28 thread ETFE 7.44 H Union, /4-28 to /4-28 Union, /4-28 to /4-28 Union, /4-28 to M-6 Union, M-6 metric to M-6 Union, /4-28 to 5/ way stopcock female luer to male luer CTFE ETFE ETFE ETFE PEEK body-nylon valve plug-polyethylene way stopcock Two female luer to male luer body-nylon valve plug-polyethylene H PTFE tubing w/ integral luer lock PTFE / CTFE Tubing, /6 O.D. x 0.08 I.D. x 0 Tubing, /8 O.D. x I.D. x 0 Tubing, /4 O.D. x 0.56 I.D. x 0 FEP FEP FEP /4-28 tubing nut for /6 O.D. tubing /4-28 tubing nut for /8 O.D. tubing ETFE ETFE Ferrule for /6 O.D. tubing Ferrule for /8 O.D. tubing Ferrule for.8 mm O.D. tubing ETFE ETFE ETFE H TYPICAL ASSEMBLIES H

12 DISPOSAFLEX Polypropylene Disposable Columns The DISPOSAFLEX column is an inexpensive, multi-purpose column designed to satisfy a wide range of analytical separations using aqueous buffers. The disposable feature eliminates the possibility of cross contamination when using labeled compounds. A typical application of the Disposaflex column is the extraction of the cyclic nucleotides camp and cgmp from sample preparations. The 8 mm inner diameter of the polypropylene column is uniform, assuring reproducibility. The 200 mm length of the DISPOSAFLEX column can easily be cut to a shorter length. The polypropylene reservoir has a 9 ml capacity. The lower column fitting with a luer outlet is molded polypropylene with a specially designed recess to securely hold a porous polyethylene bed support or a glass wool plug. Supplied unassembled with a column, polypropylene reservoir and lower column fitting. Polyethylene bed supports and on-off valves are available as accessories. Specifications Column I.D.: 8mm Column Length: 200mm Reservoir Capacity: 9mL Column Capacity: 8mL Overall Length: 265mm Bed support Luer fitting Catalog Number Description Qty DISPOSAFLEX Column Set, Unassembled 00 Replacement Parts Reservoir Only Luer Fitting Only Luer Fitting Only Shown with Optional Three-Way Valve Accessory Parts Polyethylene Bed Support, 0-50 Microns Polyethylene Bed Support, 20 Microns Way On-Off Valve, Polycarbonate/Polyethylene Way Valve, Polycarbonate/Polyethylene Way Valve, Nylon/Polyethylene Way Valve, Nylon/Polyethylene Column Outlet Cap 00 DISPOSAFLEX Disposable Glass Columns A multi-purpose glass column. Supplied assembled with a polypropylene reservoir, a lower column fitting with a luer outlet and a 20 micron polyethylene bed support. Specifications Column I.D.: 8mm Column Length: 50mm Reservoir Capacity: 9mL Column Capacity: 6mL Overall Length: 25mm Catalog Number Description Qty DISPOSAFLEX Column Set, Assembled DISPOSAFLEX Column Set, Assembled 5 Replacement Parts Polyethylene Bed Support, 20 Microns 00 Accessory Parts Polyethylene Bed Support, 0-50 Microns On-Off Valve, Polycarbonate/Polyethylene On-Off Valve, Polycarbonate/Polyethylene On-Off Valve, Polyethylene/Nylon On-Off Valve, Polyethylene/Nylon Column Outlet Cap

13 Custom Column Specifications Socket Joint Beaded Top Threaded Outer / Ground Inner The Custom Glass Shop has the ability to custom fabricate chromatography columns to your specifications. Columns are available with any standard taper, spherical or threaded joints. Consider the following issues when designing your columns. Outer Ts Joint (C) A Column OD: Column OD (A) Effective Length (B) (F) Fritted Disc Special columns will be constructed using medium wall tubing unless otherwise specified. B Column Effective Length: Round Bottom (D) Effective length is the length of the straight sides of the column prior to rounding off the tubing for sealing the top joint or the lower stopcock. On a fritted column, the effective length is measured to the fritted disk. Flat Bottom (E) Drip Tip C Top Joint Style and Size: Stopcock (Glass or PTFE Stopcock Plug) D Lower Column Configuration: Please specify round or flat bottom. E Stopcock Type and Size: Technical Support Product Design Graduating Calibrating Quartz Graded Seals Grinding Polishing and more... CGS is the Custom Glass Shop at Kimble Chase. We can be your single source for custom laboratory glassware. Whether you want a slight variation of a standard product or a completely unique design, CGS can do it! Our product design team and veteran glassblowers will meet your requirements and exceed your expectations. T: customglass@kimble-chase.com Please specify stopcock bore. PTFE, Glass or plain drip tip. F Fritted Disc Required: Special columns will be constructed using a coarse porosity (40-60 micron) fritted disc unless otherwise specified. G Plastic Coated Column: H Volume: I Quantity:

14 Columns with PTFE Stopcock Plugs A general purpose gravity column for analytical separations. A plug of glass wool can be used to support the column packing. PTFE stopcock plugs, and , control the flow rate. Custom sizes and plastic safety coating are available upon request. Catalog Column I.D. x Volume Stopcock Number Length (mm) (ml) Bore (mm) Qty Custom sizes and plastic safety coating are available upon request. See page for help specifying custom column options x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x Replacement Parts Stopcock Plug Only, 2mm Stopcock Plug Only, 4mm Packing Reservoirs with Standard Taper Joints These heavy duty reservoirs are designed for use with and series chromatography columns with and without fritted glass supports or in combination with series flow control adapters. Reservoirs are available in a variety of sizes and joint configurations. Upper Lower Catalog Standard Taper Standard Taper Capacity Number Joint Joint (ml) Qty /40 24/ /40 24/ /40 24/ /40 24/ /40 24/ /50 45/

15 Columns with Fritted Discs and PTFE Stopcock Plugs Catalog Column I.D. x Volume Stopcock Number Length (mm) (ml) Bore (mm) Qty x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x Columns with PTFE Stopcock Plugs and Standard Taper Joints Gravity chromatography columns feature PTFE stopcocks and standard taper joints and are manufactured from medium wall tubing. Glass wool (not supplied) may be used as a support for column packing material. These columns may be used alone or in combination with series reservoir and series flow control adapters. PTFE stopcock plugs, and , control the flow rate. Catalog Column I.D. x Upper Stopcock Number Length (mm) Volume (ml) Standard Taper Joint Bore (mm) Qty x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / Gravity column manufactured from medium wall tubing with PTFE stopcock similar in design to the series. These columns have a coarse porosity glass frit (40-60 micron pore size) at the lower end to act as a support for column packing material. PTFE stopcock plugs, and , control the flow rate Custom sizes and plastic safety coating are available upon request. See page for help specifying custom column options.

16 Columns with Fritted Discs, PTFE Stopcock Plugs and Standard Taper Joints Gravity column manufactured from medium wall tubing with PTFE stopcock similar in design to the series. These columns have a coarse porosity glass frit (40-60 micron pore size) at the lower end to act as a support for column packing material. These columns may be used alone or in combination with series reservoir and series flow control adapters. Custom sizes and plastic safety coating are available upon request. Catalog Column I.D. x Volume Upper Stopcock Number Length (mm) (ml) Joint Bore (mm) Qty Custom sizes and plastic safety coating are available upon request. See page for help specifying custom column options x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x / x /50 4 Replacement Parts Stopcock Plug only, 2 mm Stopcock Plug only, 4 mm Flow Control Adapters with Standard Taper Joints The flow control adapter features Kontes HI-VAC PTFE plug designed with a precision external thread for fine control. The accurately controlled valve seat and the extended tip on the PTFE plug allow semi-needle valve control for liquids. The FKM o-ring is shielded from direct exposure to liquids within the system by PTFE ring seals. Catalog Valve Plug Bore Range Number Joint (mm) Qty / / Replacement Plug Valve Plug only, 4 mm

17 Columns with Reservoirs, PTFE Stopcock Plugs and Standard Taper 24/40 Joints Gravity chromatography column manufactured from medium wall tubing with PTFE stopcock and in-line reservoir for containing liquid buffer. Glass wool (not supplied) may be used as a support for the packing material. These columns may be used alone or in combination with flow control adapter. Custom sizes and plastic safety coating are available upon request. Catalog Volume Column ID x Reservoir Stopcock Number (ml) Length (mm) Cap (ml) Bore (mm) Qty x x x x x x x x x x x x x x x x x x x x x x Replacement Parts Stopcock Plug only, 2 mm Stopcock Plug only, 4 mm Columns with Fritted Discs, Reservoirs and Standard Taper 24/40 Joints Chromatography columns manufactured from medium wall tubing with PTFE stopcock and in-line reservoir for containing liquid buffer. These columns have a coarse porosity glass frit (40-60 micron pore size) as a support for column packing material. These columns may be used alone or in combination with flow control adapter. Custom sizes and plastic coating are available upon request. Catalog Column I.D. x Volume Reservoir Stopcock Number Length (mm) (ml) Cap (ml) Bore (mm) Qty x x x x x x x x x x x x x x x x x x x x x x x Replacement Parts Stopcock Plug only, 2 mm Stopcock Plug only, 4 mm

18 Shorty Standard Lid Rectangular TLC Developing Tanks TLC Developing Tanks are manufactured from sturdy molded glass bricks that will withstand regular use for many years. The clear sides allow unobstructed visual inspection of TLC plates up to 20 x 20 cm in size. The top of the tanks are ground flat to match the lids and the edges are beveled to remove any sharp edges. The bottoms are ground to provide a flat, level surface. A raised ridge along the inside bottom allows the simultaneous development of two 20 x 20 cm or four 5 x 20 cm plates. Inside dimensions of the tanks are approximately 27cm W x 7cm D x 25cm H (0 5/8 x 2 7/8 x 9 /4 inches). Weight is approximately 5.4 kg (2 pounds). The tank lids are available in three styles: standard, nonslip and latch. Nonslip lids have a knob for easy lifting and PTFE retainers inside to prevent the lid from accidentally sliding off the tank. Latch lids are designed to hold them firmly to the developing tank, greatly improving the stability of the saturated environment for developing TLC plates. Latch lids also have a knob for easy removal and PTFE retainers. Lids in all three styles are available with holes drilled for #0 stoppers to allow addition of reagents without disturbing the equilibrium of the saturated environment. The multiplate aluminum rack allows the simultaneous development of up to six 20 x 20 cm TLC plates. The rack is hard anodized to prevent corrosion Nonslip Lid TLC Tanks with Standard Lids SHORTY tank was developed for use with 0 cm x 0 cm plates but it will also accommodate 5 x 0 or 0 x 20 plates, turned long side down. Both tanks have a raised ridge to support plates during development and are supplied with lids. Catalog Use with Plate Size Number Description W x H (mm) Qty TLC Developing Tank with Standard Lid 20 x TLC Developing Tank, Shorty with Standard Lid 0 x 20 Accessory Part TLC Standard Lid 0 x 20 or 20 x Latch Lid TLC Tank with Nonslip Lid Catalog Number Description Qty TLC Developing Tank with Nonslip Lid TLC Nonslip Lid TLC Developing Tank Only TLC Tank with Latch Lid Catalog Number Description Qty TLC Developing Tank with Latch Lid Latch Lid Rack Included TLC Latch Lid TLC Developing Tank Only, Latch TLC Tank with Latch Lid and Aluminum Rack Catalog Number Description Qty TLC Developing Tank with Latch Lid and Aluminum Rack TLC Latch Lid TLC Developing Tank Only, Latch TLC Aluminum Rack, Holds Six 20x20 cm Plates

19 Cylindrical TLC Developing Tanks with Lids Cylindrical glass tanks for the development of x in. to 0 x 20 cm plates. These tanks provide maximum vapor phase saturation with a minimum amount of solvent. Catalog For Number Plate Size Lid Qty x 0 cm Glass x 20 cm Glass x 20 cm Glass x in Polyethylene x in Polyethylene x 0 cm Polyethylene x 0 cm Polyethylene x 20 cm Polyethylene x 20 cm Polyethylene Polyethylene Cap For x in. Tanks Polyethylene Cap For 5 x 0 cm and 5 x 20 cm Tanks Glass Cap for 5 cm Tanks

20 TLC Plate Streaker A simple device for uniformly streaking TLC plates. Unit consists of a stainless steel base with an adjustable holder for a glass reservoir. To operate, the streaker is placed over edge of the counter top and the plate to be streaked is positioned parallel to it. The bent capillary tip is then adjusted so that it lightly touches the plate. The reservoir is filled with the solution to be chromatographed and by moving the unit back and forth along the counter edge, a uniform streak is accomplished. Supplied complete with glass, stainless steel capillaries and instructions. Reference: Journal of Chemical Education, Vol. 47, No. 5, May 970, page 404, A Simple Streaker for Preparative Layer Chromatography, L.J. Altman, James R.Trudell. Catalog Approx. Dimensions Number L x W x H (cm) Qty x 5 x Base Assembly Reservoir Glass Capillary Set, (2 Small, 2 Large) Stainless Capillary Set, ( Small, Large) TLC Labeling Template A transparent labeling template designed to fit over TLC plates without disturbing the absorbent layer. The apparatus features spotting guide ruler, compound and solvent travel distance markers and circles for estimation of spot sizes. Origin and 0 cm bold reference lines are provided to enable fast calculation of Rf-values. Catalog Approx. Dimensions Number L x W x H (cm) Qty x 20 x TLC Spotting Capillaries, Borosilicate Glass, Microcaps Microcaps are ingeniously simple in concept and in use. They are precisionbore glass capillary tubes, cut to predetermined lengths, so that each capillary tube will hold a known volume of fluid when filled. Microcaps are so inexpensive that it is not impractical to dispose of each tube after use. TLC Spotting Capillaries, 5 Pack, Borosilicate, Microcaps The Microcaps 5 Pack is ideal for use in spotting TLC plates. Each kit has 5 dispenser vials containing 00 micropipets each of 0.5,, 2, 5 and 0 µl sizes. Also included are a bulb assembly and spotting holder. Catalog Number Qty Filling is simple. Capillary action - not externally applied suction - draws the fluid into the tube. To dispense the fluid, simply squeeze the bulb. No unusual skills or training required. Immediate, professional results. Ideal for spotting preadsorbent TLC plates. Supplied in packages containing one bulb assembly and one dispenser vial with 00 micropipets. Volumetric tolerance is ±%. Catalog Relative Relative Number Capacity (µl) Length (mm) I.D. (in.) O.D. (in.) Qty

21 TLC Reagent Sprayers with Screw Thread Ground Joints The connection between the sprayer head and the flask is made with a special screw thread ground joint, connecting cap and loosening ring. If the joint ever freezes from reagent crystallization or residue formation, simply unscrew the connecting cap back onto the unique loosening ring and the joint will easily come apart. These sprayers will operate on as little as psi (0.07 kg/cm 2 ) air pressure with optimal results at -5 psi ( kg/cm 2 ). The connecting cap, loosening ring and o-ring should be removed before autoclaving. Approx. Height Catalog Flask x Number Capacity (ml) Joints OD (mm) Qty /22 55 x /22 47 x /22 74 x / x / x /40 27 x TLC Sprayer Head for 0 and 25 ml flasks TLC Sprayer Head for 50 ml flasks TLC Sprayer Head for 25 ml flasks TLC Sprayer Head for 250 ml flasks TLC Sprayer Head for 500 ml flasks TLC Sprayer Flask, 0 ml TLC Sprayer Flask, 25 ml TLC Sprayer Flask, 50 ml TLC Sprayer Flask, 25 ml TLC Sprayer Flask, 250 ml TLC Sprayer Flask, 500 ml Connecting Cap, Size 9, ( supplied) Loosening Ring, Size 9, ( supplied) The loosening ring and joint are easily separated for cleaning. TLC Reagent Sprayers with Standard Ground Joints These units operate from -5 psi ( kg/cm 2 ). Their all-glass construction makes them completely steam autoclavable. Approx. Height Catalog Working Standard Taper x Number Capacity (ml) Joints OD (mm) Qty /20 0 x /40 75 x / x / x TLC Sprayer Only for 0 ml Tube TLC Sprayer Only for 250 ml Flask Flask, 25 ml, Standard Taper 24/40 outer joint Flask, 250 ml, Standard Taper 24/40 outer joint

22 9590 ULTRA-WARE HPLC Economy Cap Systems The cap body is manufactured from PTFE and is supplied with a TFE/propylene o- ring and a polypropylene screw collar. The connecting threads on the top of the cap use standard /4-28 flangeless fittings. Two of the ports have /8 through holes, the third port has a /6 through hole. Assembly instructions are included. The ULTRA-WARE Flat Bottom Reservoirs are manufactured from Type I, Class A borosilicate glass with a plastic safety coating. The plastic coating also blocks virtually all UV light up to 85 nm, preventing photodegradation of light sensitive mobile phases. Economy Three Hole Cap Systems are supplied with: ea. Economy Three Hole Cap ea. Flangeless Ferrule, ETFE, / series ea. Ultra-Ware Flat Bottom Reservoir ea. Nut Plug, ETFE, / ea. All PEEK Inlet/Sparge Filter, 2µm ea. FEP Tubing, ea. Flangeless Nut, ETFE, /4-28. /8 /8 OD x /6 ID x 0 ft. Catalog Number Description Qty Economy Three Hole Cap System with Liter Reservoir Economy Three Hole Cap System with 2 Liter Reservoir Economy Three Hole Cap System with 5 Liter Reservoir Economy Three Hole Cap System with 0 Liter Reservoir Replacement Parts for Economy Three Hole Caps Replacement Economy Three Hole Cap (Body and Screw Collar) Body, PTFE Fittings Kit Kit consists of 20 of /8 OD FEP tubing, 20 CTFE ferrules and 20 (/4-28) ETFE nuts. Not pictured. Catalog Number Qty. Economy Three Hole Cap Solvent Bottle Adapter Safety System Eliminates the safety hazards of aluminum foil-wrapped solvent containers. This unique Solvent Bottle Adapter is made from PBT and converts ULTRA-WARE GL 45 mobile phase caps to standard 4L solvent bottles for direct connection to your HPLC pump. ULTRA-WARE Economy Three Hole Cap is manufactured from PTFE with a TFE/ propylene o-ring and a polypropylene screw collar. Three connecting threads on top of the cap use standard /4-28 flangeless fittings. Two ports have /8 through holes for connection to the inlet / sparge filter. The third port has a /6 through hole used as a vent port during sparging. Each Safety System consists of the following: Port for sparging line (optional use) Filter Catalog No Draws solvent from bottom of solvent bottle Port for solvent intake line Filters incoming sparging gas (optional use) ea. Economy Three Hole Cap, GL ea. Solvent Bottle Adapter, GL 45 (Systems available with or without adapter. See below.) ea. Combination Inlet / Sparge Filter, 2µm, PEEK ea. Flangeless Nut, ETFE, /4-28 x /8 (2 Req d.) ea. Flangeless Ferrule, ETFE, /8 (Optional - 2 Req d.) ea. Vent Port Plug, ETFE, /4-28 ( Req d.) ea. Tubing, FEP, /8 O.D. x /6 I.D. x 0 ft. Catalog Number Description Safety System with GL 45 Bottle Adapter Safety System without GL 45 Bottle Adapter Unique Filter Combines Functions to Increase Efficiency Included with your Safety System is the unique Bottom-of-the-Bottle Inlet / Sparge Filter. This component combines the functions of an inlet filter with a sparger in a single, convenient device. Sparging bubbles are prevented from entering the mobile phase stream while allowing the pump to draw all but a few milliliters of solvent from the reservoir or bottle. All PEEK construction makes it ideal for virtually all mobile phases. Supplied with 2µm porosity frits and connections for /8 O.D. tubing

23 Plastic-Coated ULTRA-WARE Reservoirs ULTRA-WARE Reservoirs have been specially designed for the preparation, storage and delivery of all liquid chromatography mobile phases. Each ULTRA-WARE Reservoir is manufactured from the most chemically inert glass available to prevent the leaching of any extractables into the mobile phase solvents. They are then externally coated with plastic containing an added ultraviolet (UV) blocking agent. In case of an accident, the plastic safety coating helps retain glass fragments and allows a reasonable amount of time for the safe disposal of the liquid contents. The plastic coating also blocks virtually all UV light up to 85 nm, preventing photodegradation of light sensitive reagents. ULTRA-WARE Reservoirs are recommended for use under vacuum for filtration and vacuum degassing of mobile phase solvents. ULTRA-WARE Reservoirs can also be used at internal pressures up to 6 psig for helium sparging and blanketing of the mobile phase. The conical bottom reservoirs deliver virtually all of the mobile phase without dangerous reservoir tilting. All ULTRA-WARE Reservoirs are supplied with GL 45 screw thread. *Patent No. Des. 292, Conical Bottom HPLC Reservoirs without Graduations Catalog Number Capacity Height x Dia. Qty ml 20 x 85 mm ml 20 x 05 mm liter 275 x 0 mm liter 9 x 50 mm liter 7 x 205 mm liter 4 x 255 mm liter 578 x 5 mm Conical Bottom HPLC Reservoirs with Graduations Catalog Number Capacity Height x Dia. Qty ml 20 x 85 mm ml 20 x 05 mm liter 275 x 0 mm liter 9 x 50 mm liter 7 x 205 mm liter 4 x 255 mm liter 578 x 5 mm Conical Bottom HPLC Reservoirs with Side Necks and Graduations Side neck allows addition of filtered solvent without removing delivery cap. Catalog Number Capacity Height x Dia. Qty liter 9 x 50 mm liter 7 x 205 mm liter 4 x 255 mm liter 578 x 5 mm Flat Bottom HPLC Reservoirs without Graduations Catalog Number Capacity Height x Dia. Qty ml x 7 mm ml 65 x 89 mm liter 205 x mm liter 250 x 8 mm liter 20 x 86 mm liter 400 x 24 mm liter 490 x 00 mm Flat Bottom HPLC Reservoirs with Graduations Catalog Number Capacity Height x Dia. Qty ml 20 x 85 mm ml 20 x 05 mm liter 275 x 0 mm liter 9 x 50 mm liter 7 x 205 mm liter 4 x 255 mm liter 578 x 5 mm Specifications ULTRA-WARE HPLC Reservoirs Materials: Reservoir: Type I, Class A, Borosilicate Glass Plastic Coating: PVC with UV blocking agent Screw Thread: GL 45 Operating Pressure: -.0 to 0.4 bar UV Transmittance: <% up to 85 nm

24 ULTRA-WARE Solvent Pickup Adapter Designed to be used with the Five Valve, Four Valve and Filtration caps to provide safe, in-line filtration that replaces the traditional pour-and-wait funnel filtration method. The unique BEVEL-SEAL makes a vacuum tight o-ring connection to the PTFE tubing. The HI-VAC valve is easier to open under vacuum than the standard stopcock. Closing the valve provides vacuum degassing after filtration. Each unit is supplied with feet (9 cm) of /4 O.D. FEP tubing. Valve o-ring size is 00 and sidearm o-ring size is 08. Catalog Number Description Qty Pickup Adapter, Standard, 47 mm Pickup Adapter, THF-Resistant, 47 mm Pickup Adapter, Standard, 90 mm Pickup Adapter, THF-Resistant, 90 mm Replacement Parts () Valve Plug Assembly, Std (2) Glass Pickup Adapter Body, 47mm (2) Glass Pickup Adapter Body, 90mm () Cap, Nylon, 5-45, 8.5mm 2 2 ULTRA-WARE Three Hole Delivery Cap Three holes have /4-28 threads top and bottom with /8 I.D. holes through to accept /8 O.D. PTFE tubing. Body is PTFE. Supplied complete with three /4-28 CTFE plugs and a TFE / propylene o-ring. Catalog Cap Number Description Thread Qty Three Hole Cap GL 45 Replacement Parts () Plug, ETFE, / (2) Screw Cap, GL ULTRA-WARE Three Valve Delivery Cap Three on-off CTFE valves have /4-28 threads top and bottom. Valve body is PTFE. Valve stem is CTFE. O-ring material for valve stem and lower seal is TFE / propylene. Catalog Cap Number Description Thread Qty Three Valve Cap GL 45 Replacement Parts () Valve Handle, Blue () Valve Handle, Red () Valve Handle, Yellow (2) Valve Stem, CTFE, Short (4) Screw Cap, GL 45 THF-resistant versions of ULTRA-WARE caps are also available. Please call for more information

25 ULTRA-WARE Filtration Cap Body is constructed of glass-filled PTFE with a vacuum adapter for /4 I.D. tubing. Upper screw clamp holds a solvent pickup adapter or funnel. Coarse fritted glass filter support is removable. Supplied with a PTFE / propylene o-ring. Catalog Filter Cap Number Diameter Thread Qty mm GL 45 2 Replacement Parts () Upper Screw Clamp, 47 mm (2) Coarse Porosity Glass Support Frit, 47 mm () Hose Barb, PFA, / (4) Drip Tip, PTFE (5) Lower Cap Retainer Ring (6) Lower Screw Cap, GL 45 4 Filtration cap, shown with Solvent Pickup Adapter, , and ULTRA-WARE, safety-coated reservoir, 9590-series, available for purchase separately. The safety coating contains an ultraviolet (UV) blocking agent to help protect solvents from degradation ULTRA-WARE Four Valve Filtration / Delivery Cap Cap combines the mobile phase filtration, sparging / degassing, storage and delivery functions. Tubing connections are made in the back of the cap, reducing the typical clutter of tubing at the reservoirs. An integral check valve in the sparge port prevents the mobile phase from backing up into the gas lines. A pressure release valve prevents the reservoir from being accidentally over-pressurized. The /4-28 fitting connections allow easy connection to any HPLC pump system. Cap body is manufactured from glass-filled PTFE. Cap will fit any reservoir or bottle with a GL 45 thread. Patent No. 5,97,467 Catalog Filter Cap Number Diameter Thread Qty mm GL 45 Replacement Parts () Upper Screw Clamp, 47 mm (2) Coarse Porosity Glass Support Frit, 47 mm (4) Valve Stem, CTFE, Short (for Sparge and Pump Ports) (5) Drip Tip, PTFE (6) Check Valve with Retainer (7) Lower Cap Retainer Ring (8) Lower Screw Cap, GL THF-resistant versions of ULTRA-WARE caps are also available. Please call for more information

26 Five valve cap, shown with Solvent Pickup Adapter, , and ULTRA- WARE, safety-coated reservoir, 9590-series, available for purchase separately. The safety coating contains an ultraviolet (UV) blocking agent to help protect solvents from degradation. ULTRA-WARE Five Valve Recirculation / Filtration Cap Designed to permit the recirculation of mobile phase back into the reservoir while maintaining the helium sparged environment. Cap also performs stepwise filtration, sparging / degassing, storage and delivery to the HPLC pump. An integral check valve prevents mobile phase from backing up into the gas lines. A pressure release valve protects the reservoir from being accidentally over-pressurized. The /4-28 fitting connections allow easy connection to any HPLC pump system. Cap body is manufactured from glass-filled PTFE. Cap will fit any reservoir or bottle with a GL 45 thread. Patent No. 5,97,467 Catalog Filter Cap Number Diameter Thread Qty mm GL 45 Replacement Parts () Upper Screw Clamp, 47 mm (2) Coarse Porosity Glass Support Frit, 47 mm (4) Valve Stem, CTFE, Short (for Sparge and Pump Ports) (5) Drip Tip, PTFE (6) Check Valve with Retainer (7) Lower Cap Retainer Ring (8) Lower Screw Cap, GL 45 THF-resistant versions of ULTRA-WARE caps are also available. Please call for more information. 2 Coarse Glass Frit Screw Clamp 90 mm Conversion Base for ULTRA-WARE Caps Converts the Five Valve, Four Valve and Filtration caps to use 90 mm filter membranes, providing up to four times faster filtration. The conversion base is manufactured from glass-filled PTFE and is supplied with a coarse porosity glass support frit and an upper screw clamp. Requires a 90 mm Solvent Pickup Adapter, or THF-resistant Catalog Number Description Qty mm Conversion Base Replacement Parts () Upper Screw Clamp, 90mm (2) Glass Support Frit, Coarse, 90 mm For a complete listing of chromatography columns and fittings, visit our website:

27 ULTRA-WARE Fittings Kit, Standard Version This kit contains all of the tube fittings, adapters and solvent inlet filters required to plumb any ULTRA-WARE cap to virtually all HPLC pumps. Not recommended for use with THF or chlorinated hydrocarbons. Catalog Number Description Qty Fittings Kit, Standard Version Quantity Component in Parts Description Kit Qty Adapter, F /4-28 to F-Luer Adapter, M /4-28 to M-Luer Union, 5/6-24 to / Tubing Nut, /4-28, for /8 O.D Tubing Nut, M6, for /8 O.D Tubing Nut, 5/6-24, for /6 O.D Ferrule, for /8 O.D Ferrule, for /6 O.D Ferrule, for.8 mm O.D Inlet Filter, for /8 O.D. Tubing Inlet Filter, Bottom-of-the-Bottle TM, for /8 O.D. Tubing Adapter, /4-28 to /4 Hose Barb ULTRA-WARE Fittings Kit, THF-Resistant This kit contains all of the tube fittings, adapters and solvent inlet filters required to plumb any ULTRA-WARE cap to virtually all HPLC pumps. However, it is designed to be used specifically with THF or other very aggressive organic solutions. Catalog Number Description Qty Fittings Kit, THF Version Quantity Component in Parts Description Kit Qty Adapter, F /4-28 to F-Luer Adapter, M /4-28 to M-Luer Union, 5/6-24 to / Tubing Nut, /4-28, for /8 O.D Tubing Nut, M6, for /8 O.D Tubing Nut, 5/6-24, for /6 O.D Tubing Nut, /4-28, for /8 O.D Ferrule, for /8 O.D Ferrule, for /6 O.D Ferrule, for.8 mm O.D Inlet Filter, BOTB, for /6 I.D Inlet Filter, for /6 I.D Adapter, /4-28 to /4 Hose Barb

28 Inlet / Sparge Filter The inlet/sparge filter combines an inlet filter with a sparger in a single, convenient device. The all PEEK construction is ideal for virtually all mobile phases. The special design prevents sparging bubbles from entering the mobile phase stream while allowing the pump to draw all but a few milliliters of solvent from the reservoir. The inlet/ sparge filter is supplied with 2 µm porosity frits and connections for /8 O.D. tubing. Catalog O.D. x Fits Tubing Number Length Porosity Size Qty x µm /8 O.D ULTRA-WARE Inlet Filter, Bottom-of-the-Bottle TM A specially designed inlet filter that allows solvent to be drawn to within 2 mm of the bottom of the filter. Combined with the ULTRA-WARE conical bottom reservoir, this filter will utilize all but a few milliliters of mobile phase for more efficient use of your solvent. Catalog O.D. x Fits Tubing Number Length Size Qty /2 x /4 /8 O.D /2 x /6 I.D ULTRA-WARE Stainless Steel Solvent Inlet Filter Two micron porosity filter with a CTFE nut for connection to /8 O.D. PTFE tubing. Recommended for use with the 250 ml to 5 liter size reservoir systems. Catalog O.D. x Number Length Qty /2 x SS Filter Only CTFE Nut ULTRA-WARE Stainless Steel Solvent Inlet Filter Ten micron porosity all stainless steel filter with a stem for slip connection to PTFE tubing. Recommended for use with THF-resistant systems. Catalog O.D. x Stem Fits Tubing Number Length O.D. Size Qty /2 x /6 /8 O.D /2 x /8 /6 O.D. ULTRA-WARE Stainless Steel Solvent Inlet Filter Ten micron porosity filter with an ETFE nut and ferrule for connection to /8 O.D. PTFE tubing. A CTFE hose barb adapter for connection to /4 O.D. (5/2 I.D.) tubing is also included. Recommended for use with 0 and 20 liter size reservoir systems. Catalog O.D. x Number Length Qty /8 x / Filter Only ULTRA-WARE Glass Solvent Inlet Filter Five micron porosity filter with a CTFE union, /4-28 nut and ferrule for connection to /8 O.D. tubing. Catalog O.D. x Number Length Qty /2 x 2 H Union, /4-28, CTFE

29 Microfiltration Assembly, 25 mm, with Support, ULTRA-WARE The 25 mm vacuum microfiltration assemblies are designed to handle small volumes of liquids for analysis of particulate or microbiological contamination. The funnel is graduated from 5 to 5 ml in ml increments. Prefilter size is 6 mm diameter and the approximate filter area is 2.5 cm 2. Connection to our 25 ml filtration flask (available below as an accessory) is made with a No. 5 silicone stopper. with Fritted Glass Support Used for general filtration. Unit is supplied with a coarse fritted glass support base, 5 ml graduated funnel, anodized aluminum clamp and a No. 5 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Fritted Glass x () Glass Funnel, 25 mm, 5 ml capacity (2) Anodized Aluminum Clamp, 25 mm () Fritted Glass Support Base, 25 mm (4) No. 5 Silicone Stopper, /8 (9.5 mm) Hole, for Use with Accessory 25 ml Filtration Flask Accessories Glass Funnel, 25 mm, 50 ml Capacity Glass Funnel, 25 mm, 00 ml Capacity Filtration Flask, 25 ml, No. 5 Rubber Stopper Joint, #2 Hose Connection No. 8 Silicone Stopper, /8 (9.5 mm) Hole, for Use with 000 ml Filtration Flask Filtration Flask, 000 ml, No. 8 Stopper Joint, /4 Hose Connection with Stainless Steel Support Used for filtering viscous or proteinaceous solutions or to produce ultra clean filtrate. This unit is supplied with a 6 stainless steel support screen, PTFE support screen gasket, glass support base, 5 ml graduated funnel, anodized aluminum clamp and a #5 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Stainless x () Glass Funnel, 25 mm,5 ml Capacity (2) Anodized Aluminum Clamp, 25 mm () Stainless Steel Support Screen, 25 mm (4) PTFE Support Screen Gasket, Pkg./25 ( Supplied) (5) Glass Support Base, 25 mm (6) No. 5 Silicone Stopper, /8 (9.5 mm) Hole, for Use with Accessory 25 ml Filtration Flask Accessories Glass Funnel, 25 mm, 50 ml Capacity Glass Funnel, 25 mm, 00 ml Capacity Flask, 25 ml, No. 5 Rubber Stopper Joint, No. 2 Hose Connection No. 8 Silicone Stopper, /8 (9.5 mm) Hole, for Use with 000 ml, Filtration Flask Filtration Flask, 000 ml, No. 8 Stopper Joint, /4 Hose Connection

30 Accessory Flasks Filtering Flask, ULTRA-WARE This flask is manufactured from heavy wall borosilicate glass to assure the mechanical strength needed for vacuum filtration. The side arm accepts standard /4 (6 mm) I.D. tubing for connection to vacuum sources. A second flask should be connected between the filtering flask and the vacuum source to prevent accidental entry of the filtrate into the vacuum line or pump. Kontes Article No. Description Qty Flask, 25 ml, #5 Rubber Stopper Joint, #2 Hose Connection Flask, 000 ml, #8 Rubber Stopper Joint, #2 Hose Connection Flask, 2000 ml, #8 Rubber Stopper Joint, #2 Hose Connection Flask, 4000 ml, #8 Rubber Stopper Joint, #2 Hose Connection Abbreviation Plastic Resin Codes Chemical Designation Temp o C (Max) ABS Acryl-Butadienestyrene Acetal NO YES NO YES YES YES LDPE Low Density Polyethylene NO YES NO YES YES YES HDPE High Density Polyethylene NO YES NO YES YES YES NYL Polyamide 90 0 NO YES NO YES YES YES PCTFE Polychlorotrifluoroethylene 80 0 NO YES NO YES YES YES PC Polycarbonate 5 0 YES YES NO YES YES YES PP Polypropylene 5 0 YES YES NO YES NO YES PTFE Polytetrafluoroethylene YES YES YES YES YES YES PVC Polyvinyl Chloride 70 0 NO YES NO YES NO YES PVDF Kynar (polyvinyldene fluoride) 0 62 YES YES NO YES NO YES E-CTFE Ethylene Chlortrifluoroethylene YES YES YES YES NO YES ETFE EthyleneTetrafluoroethylene YES YES YES YES NO YES PFA Perfluoroalkoxy YES YES YES YES NO YES San Styrene NO YES NO YES YES YES PMP Polymethylpentene (TPX) YES YES NO YES YES YES PMMA Polymethylmetyacrylate (PMMP) 50 0 NO YES NO YES YES YES PS Polystyrene NO YES NO YES YES YES PEEK Polyetheretherketone 25 0 YES YES NO YES YES YES PVC Polyvinylchloride YES YES NO YES NO YES TFE Tetrafluoroethylene YES YES YES YES YES YES Temp o C (lmin) Autoclavable Microwavable Dry Heat Gas Gamma Disinfectants

31 Microfiltration Assembly, 47 mm, with Support, ULTRA-WARE These vacuum microfiltration assemblies are designed to handle up to 500 ml of sample liquids for the analysis of particulate or microbiological contamination. The funnel is graduated from 00 to 250 ml in 25 ml increments. Prefilter size is 5 mm and the approximate filter area is 9.6 cm 2. Connection to our liter filtration flask (available below as an accessory) is made with a #8 silicone stopper. with Fritted Glass Support Used for general filtration. This unit is supplied with a coarse fritted glass support base, 00 ml graduated funnel, anodized aluminum clamp and a #8 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Fritted Glass x () Glass Funnel, 47 mm,00 ml (2) Anodized Aluminum Clamp, 47 mm () Fritted Glass Support Base, 47 mm (4) #8 Silicone Stopper, 9/6 (4. mm) Hole, Fits Accessory L Flask Accessories Glass Funnel, 47 mm, 00 ml Capacity Glass Funnel, 47 mm, 500 ml Capacity Glass Funnel, 47 mm, 000 ml Capacity Filtration Flask, 000 ml Capacity, #8 Stopper Joint, /4 Hose Connection Filtration Flask, Safety Coated, L Cap., #8 Stop. Jt., /4 Hose Con. with PTFE-Faced Fritted Glass Support Used when autoclaving with the filter in place. The PTFE coating prevents the membrane from adhering to the ground glass surface. This unit is supplied with a PTFE faced fritted glass support base, PTFE-faced 00 ml graduated funnel, anodized aluminum clamp and a #8 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty PTFE-Faced x () PTFE-Faced Glass Funnel, 47 mm, 00 ml (2) Anodized Aluminum Clamp, 47 mm () PTFE-Faced Fritted Glass Support Base, 47 m (4) #8 Silicone Stopper, 9/6 (4. mm) Hole, Fits Accessory L Flask Accessories Filtration Flask, 000 ml Capacity, #8 Stopper Joint, /4 Hose Connection Filtration Flask, Safety Coated, L Cap., #8 Stop. Jt., /4 Hose Con with Stainless Steel Support Used for filtering viscous or proteinaceous solutions or to produce ultra clean filtrate. This unit is supplied with a 6 stainless steel support screen, PTFE support screen gasket, glass support base, 00 ml graduated funnel, anodized aluminum clamp and a #8 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Stainless x () Glass Funnel, 47 mm,00 ml (2) Anodized Aluminum Clamp, 47 mm () Stainless Steel Support Screen, 47 mm (4) PTFE Support Screen Gasket, Pkg./25, ( Supplied) (5) Glass Support Base, 47 mm (6) #8 Silicone Stopper, 9/6 (4. mm) Hole, Fits Accessory L Flask Accessories Glass Funnel, 47 mm, 00 ml Capacity Glass Funnel, 47 mm, 500 ml Capacity Glass Funnel, 47 mm, 000 ml Capacity Filtration Flask, L Capacity, #8 Stopper Joint, /4 Hose Connection Filtration Flask, Safety Coated, L Cap., #8 Stop. Jt., /4 Hose Con Accessory Flasks

32 2 4 Microfiltration Apparatus, 47 mm All-Glass, with Fritted Glass Support Base, ULTRA-WARE The 47 mm filtration apparatus with fritted glass support is recommended for routine filtration analysis of corrosive liquids and the removal of particulates from HPLC solvents. The ground joint connection eliminates the phthalate contamination that can occur when using silicone or neoprene stoppers. The support base has a coarse porosity glass frit and an integral vacuum connection that is located above the drip tip to prevent contamination of the vacuum line with filtrate droplets. Each apparatus is supplied with funnel, anodized aluminum clamp, 47 mm fritted glass support base and filtration flask. Joints are standard taper 40/5. Funnel Flask Filter Approx. Overall Kontes Cap. Cap. Size Hose Height x Width Article No. (ml) (ml) (mm) Connection (mm) Qty #2 40 x #2 55 x #2 600 x () Glass Funnel, 47 mm, 00 ml () Glass Funnel, 47 mm, 500 ml () Glass Funnel, 47 mm, 000 ml (2) Aluminum Clamp, 47 mm () Fritted Glass Support Base, 47 mm, Standard Taper 40/ (4) Flask, 000 ml, standard taper 40/ (4) Flask, 2000 ml, standard taper 40/ (4) Flask, 4000 ml, standard taper 40/ Accessories PTFE Joint Sleeve, standatd taper 40/5, 6/Pkg Glass Funnel, 47 mm, 00 ml Flask Cap, Outer Joint, standard taper 40/ Microfiltration Apparatus, 47 mm, with Stainless Steel Support, ULTRA-WARE The 47 mm filtration apparatus with stainless steel support is recommended when filtering viscous or proteinaceous solutions to give the maximum flow rate. This apparatus is also used to produce ultra clean filtrate since the stainless steel screen will not shed particles into the filtrate. Like the apparatus with fritted glass support, the ground joint connection eliminates the possible phthalate contamination that can occur when using silicone or neoprene stoppers. Each apparatus is supplied with funnel, anodized aluminum clamp, stainless steel support screen, PTFE gasket, 47 mm glass support base and filtration flask. Funnel Flask Filter Approx. Overall Kontes Cap. Cap. Size Hose Height x Width Article No. (ml) (ml) (mm) Connection (mm) Qty #2 40 x () Glass Funnel, 47 mm, 00 ml (2) Anodized Aluminum Clamp, 47 mm () Stainless Steel Support Screen, 47 mm (4) PTFE Support Screen Gasket, Pkg./25, ( Supplied) (5) Glass Support Base, 47 mm, standard taper 40/5 Outer Joint (6) Filtration Flask, 000 ml, standard taper 40/5 Inner Joint Accessories PTFE Joint Sleeve, standard taper 40/5, 6/Pkg Glass Funnel, 47 mm, 00 ml Capacity Glass Funnel, 47 mm, 500 ml Capacity Glass Funnel, 47 mm, 000 ml Capacity Filtration Flask, 2000 ml Capacity, standard taper 40/5 Inner Joint Filtration Flask, 4000 ml Capacity, standard taper 40/5 Inner Joint Flask Cap, Outer Joint, standard taper 40/

33 47 mm Filtration Assembly with Bottle and Fritted Glass Support SPECIFICATIONS with Bottle and Fritted Glass Support Materials: This filtration assembly is specially designed to collect the filtrate directly into a media-style bottle. The Funnel and Base: Borosilicate Glass Clamp: Anodized Aluminum Bottle Adapter: Borosilicate Glass fritted glass support base connects directly to the Tubing Adapter: Polypropylene with size 08 Viton o-ring bottle with a unique cap and sealing ring. The support base has sealed-in coarse porosity (40-60 µm) Caps: PBT Sealing Rings: PTFE-faced Silicone fritted glass disc. The media-style bottle is vacuum Filter Size: 47 mm diameter and pressure rated from - to.5 bar. The Filtration Filter Area: Approximately 9.6 cm 2 Assembly is supplied complete with 00 ml funnel, Prefilter Size: 5 mm diameter clamp, support base and bottle. Funnel Capacity: 00 ml maximum, graduated from 00 ml to 250 ml in 25 ml increments Connection: GL-45 thread to liter vacuum and pressure rated media-style bottle Kontes Article No. Description Qty mm Filtration Assembly with Bottle and Fritted Glass Support () Glass Funnel, 47 mm, 00 ml capacity (2) Anodized Aluminum Clamp, 47 mm () Fritted Glass Support Base, 47 mm (4) Tubing Adapter, Assembly /4 x (5) Cap, PBT, GL 45, 4 mm opening (6) Sealing Ring, Silicone/PTFE, 42 mm (7) Bottle, Vacuum/Pressure, 000 ml Accessories Glass Funnel, 47 mm, 500 ml capacity Glass Funnel, 47 mm, 000 ml capacity Diaphragm Vacuum Pump, 0/5 VAC 47 mm Filtration Assembly with Bottle and Stainless Steel Support SPECIFICATIONS Materials: with Bottle and Stainless Steel Support Funnel and Base: Borosilicate Glass Support Screen: 6 Stainless Steel This filtration assembly is similar to the one listed Screen Gasket: PTFE above except that the support base has a removable Clamp: Anodized Aluminum stainless steel support screen. This assembly Bottle Adapter: Borosilicate Glass is recommended for viscous or proteinaceous Tubing Adapter: Polypropylene with size 08 Viton o-ring Caps: PBT solutions and for producing ultra-clean filtrate. The Sealing Rings: PTFE-faced Silicone Filtration Assembly is supplied complete with 00 ml funnel, aluminum clamp, support screen, PTFE Filter Size: 47 mm diameter Filter Area: Approximately 9.6 cm 2 gasket, support base and bottle. Prefilter Size: 5 mm diameter Funnel Capacity: 00 ml maximum, graduated from 00 ml to 250 ml in 25 ml increments Connection: GL-45 thread to liter vacuum and pressure rated media-style bottle Kontes Article No. Description Qty mm Filtration Assembly with Bottle and Stainless Steel Support () Glass Funnel, 47 mm, 00 ml capacity (2) Anodized Aluminum Clamp, 47 mm () Stainless Steel Support Screen, 47 mm (4) PTFE Support Screen Gasket, 47 mm (5) Glass Support Base, 47 mm (6) Tubing Adapter, Assembly /4 x (7) Cap, PBT, GL 45, 4 mm opening (8) Sealing Ring, Silicone/PTFE, 42 mm (9) Bottle, Vacuum/Pressure, 000 ml Accessories Glass Funnel, 47 mm, 500 ml capacity Glass Funnel, 47 mm, 000 ml capacity Diaphragm Vacuum Pump, 0/5 VAC Stopper Size 5, /8 Hole

34 Adapter Assembly, GL 45 Thread This adapter assembly is designed to allow connection of a 47 mm or 90 mm filtration support base with a straight drip tip to a bottle or reservoir with a GL 45 thread. This allows the filtrate to be collected directly into a media-type bottle when using a standard membrane filtration assembly. All components are steam autoclavable. Kontes Article No. Description Qty Filtration Adapter Assembly Typical Set-up Glass Adapter Only, 2 mm Cap, PBT, GL 2, 20 mm opening Sealing Ring, Silicone/PTFE, 29mm Cap, PBT, GL 45, 4 mm opening Sealing Ring, Silicone/PTFE, 42mm Tubing Adapter, Assembly /4 x -425, # Hose Connection Accessory Parts Solvent Bottle Adapter, GL-45 Filtering Flask, Plastic Safety Coated These erlenmeyer style flasks are manufactured from heavy-wall borosilicate glass to assure the mechanical strength needed for vacuum filtration. The 25 ml flask has a No. 5 stopper joint. The, 2 and 4 liter flasks have a No. 8 stopper joint. All flasks have a #2 side arm for connection to /4 (6 mm) I.D. flexible tubing. Kontes Article No. Description Qty Filter Flask, Coated, 25 ml Filter Flask, Coated, L Filter Flask, Coated, 2L Filter Flask, Coated, 4L Accessory Filtration Assembly Grad. Funnel Filter Approx. Overall Kontes Support Capacity Size Height x Dia. Article No. Type (ml) (mm) (mm) Qty Fritted Glass x

35 Microfiltration Apparatus, 47 mm All-Glass, with Solvent Pickup Adapter, ULTRA-WARE This system eliminates pouring HPLC solvents into funnels. The solvent pickup adapter draws solventdirectly from a reagent bottle through a 47 mm filter and into a filter flask. After filtration is complete, the HI-VAC valve on the solvent pickup adapter can be turned off for vacuum degassing. Existing ULTRA-WARE and Millipore filtration assemblies are easily converted to in-line filtration/degassing by replacing existing funnels with solvent pickup adapters. They clamp to support bases in the same way that funnels do. This system comes with a solvent pickup adapter, coarse porosity fritted glass filter base, inner joint flask and an aluminum clamp. Filter base has an integral hose connection above the filtrate drip tip to prevent contamination of the vacuum line with filtrate droplets. Approx. Overall Height Filter Flask x Kontes Size Cap. Hose Standard taper Width Article No. (mm) (ml) Connection Joints (mm) Qty #2 40/5 40 x #2 40/5 525 x () Solvent Pickup Adapter with /4 OD PTFE Tubing (2) Anodized Aluminum Clamp, 47 mm () Fritted Glass Support Base, standard taper 40/5 Outer Joint (4) Flask, 000 ml, standard taper 40/5 Inner Joint (4) Flask, 4000 ml, standard taper 40/5 Inner Joint Accessory Flask Cap, Outer Joint standard taper 40/5 Custom Fabrications Quality Repairs Modifications of All Kinds No Order Too Small Quick Turnaround KONTES custom glass shop customglass@kimble-chase.com Contact the Kontes Custom Glass Shop at KIMBLE CHASE for modifications of standard products or fabrication of your unique designs. And when it comes to repairs, we can save you up to 75% of the cost of a new item. Save an additional 0% on repairs of Kontes or Kimble Brand products. your requirements to: CustomGlass@kimble-chase.com

36 Microfiltration Assembly, 90 mm, with Support, ULTRA-WARE The 90 mm vacuum microfiltration assemblies are designed for large sample volumes or samples with high particulate loads that would tend to clog a 47 mm diameter filter. Filtration rates are up to four times faster than 47 mm filters. The funnel is graduated from 00 to 000 ml in 50 ml increments. Maximum funnel capacity is 00 ml. Prefilter size is 70 mm diameter and the approximate filter area is 8.5 cm 2. Connection to our liter filtration flask (available as an accessory) is made with a #8 silicone stopper. with Fritted Glass Support Used for general filtration. Unit is supplied with a coarse fritted glass support base, liter graduated funnel, anodized aluminum clamp and a #8 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Fritted Glass x () Glass Funnel, 90 mm,000 ml (2) Anodized Aluminum Clamp, 90 mm () Fritted Glass Support Base, 90 mm (4) #8 Silicone Stopper, 9/6 (4. mm) Hole, Fits Accessory L Flask Accessories Filtration Flask, 000 ml Capacity, #8 Stopper Joint, /4 Hose Connection Filtration Flask, Safety Coated, L Cap., #8 Stop. Jt., /4 Hose Con. with Replaceable Fritted Glass Support Used for general filtration. The fritted glass support disc can be replaced if it becomes clogged or exchanged if sample to sample cross contamination is a concern. This unit is supplied with a coarse fritted glass support disc, glass support base, liter graduated funnel, anodized aluminum clamp and a #8 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Fritted Glass x () Glass Funnel, 90 mm,000 ml (2) Anodized Aluminum Clamp, 90 mm () Fritted Glass Support Disc, Coarse, 90 mm (4) Fritted Glass Support Base, 90 mm, Shell (5) #8 Silicone Stopper, 9/6 (4. mm) Hole, Fits Accessory L Flask Accessory Flask Accessories Filtration Flask, 000 ml Capacity, #8 Stopper Joint, /4 Hose Connection Filtration Flask, Safety Coated, L Cap., #8 Stop. Jt., /4 Hose Con Stopper Size 5, /8 Hole with Stainless Steel Support Used for filtering viscous or proteinaceous solutions or to produce ultra-clean filtrate. This unit is supplied with a 6 stainless steel support screen, glass support base, 00 ml graduated funnel, anodized aluminum clamp and a No. 8 silicone stopper. Graduated Filter Approx. Overall Kontes Support Funnel Size Height x Article No. Type Cap. (ml) (mm) Diam. (mm) Qty Stainless x () Glass Funnel, 90 mm,000 ml (2) Aluminum Clamp, 90 mm () Stainless Steel Support Screen, 90 mm (4) Glass Support Base, 90 mm (5) #8 Silicone Stopper, 9/6 (4. mm) Hole, Fits Accessory, L Accessories Filtration Flask, L Capacity, #8 Stopper Joint, /4 Hose Connection Filtration Flask, Safety Coated, L Cap., #8 Stop. Jt., /4 Hose Con

37 Microfiltration Apparatus, 90 mm All-Glass, with Fritted Glass Support, ULTRA-WARE The 90 mm filtration assembly with fritted glass support is designed for large volume filtration analysis of corrosive liquids or samples with high particulate loads that would tend to clog a 47 mm diameter filter. This unit is also recommended for filtering large volumes of HPLC solvents. The ground glass connection eliminates the possibility of phthalate contamination that can occur when using silicone or neoprene stoppers. Each apparatus is supplied with 000 ml funnel, 90 mm coarse porosity fritted glass support base, anodized aluminum clamp and filtration flask. Kontes Article No. Description Qty mm Filtration Apparatus with 000 ml Funnel and liter Flask mm Filtration Apparatus with 000 ml Funnel and 2 liter Flask mm Filtration Apparatus with 000 ml Funnel and 4 liter Flask () Glass Funnel, 90 mm, 000 ml capacity (2) Anodized Aluminum Clamp, 90 mm () Fritted Glass Support Base, 90 mm, 40/5 outer joint (4) Filtration Flask, 000 ml capacity, 40/5 inner joint (4) Filtration Flask, 2000 ml capacity, 40/5 inner joint (4) Filtration Flask, 4000 ml capacity, 40/5 inner joint Accessories PTFE Joint Sleeve, 40/5, 6/pkg Flask Cap, 40/5 outer joint Microfiltration Apparatus, 90 mm All-Glass, with Replaceable Fritted Glass Support, ULTRA-WARE The 90 mm filtration apparatus with replaceable fritted glass support is recommended for large volume filtration analysis of corrosive liquids or samples with high particulate loads. The glass support disc can be replaced if it becomes clogged or if sample to sample contamination is a concern. Like the apparatus with fritted glass support, the ground joint connection eliminates the possibility of phthalate contamination that can occur when using silicone or neoprene stoppers. Each apparatus is supplied with 000 ml funnel, 90 mm glass support base, coarse porosity fritted glass disc, anodized aluminum clamp and filtration flask. Kontes Article No. Description Qty mm Filtration Apparatus with 000 ml Funnel and liter Flask mm Filtration Apparatus with 000 ml Funnel and 2 liter Flask mm Filtration Apparatus with 000 ml Funnel and 4 liter Flask () Glass Funnel, 90 mm, 000 ml capacity (2) Anodized Aluminum Clamp, 90 mm () Fritted Glass Support Disc, 90 mm (4) Glass Support Base, 90 mm, standard taper 40/5 outer joint (5) Filtration Flask, 000 ml capacity, standard taper 40/5 inner joint (5) Filtration Flask, 2000 ml capacity, standard taper 40/5 inner joint (5) Filtration Flask, 4000 ml capacity, standard taper 40/5 inner joint Accessories PTFE Joint Sleeve, standard taper 40/5, 6/pkg Flask Cap, standard taper 40/5 outer joint

38 2 4 5 Microfiltration Apparatus, 90 mm, with Stainless Steel Support, ULTRA-WARE The 90 mm filtration apparatus with stainless steel support is recommended when filtering large volumes of viscous or proteinaceous solutions to give the maximum flow rate. This unit is also used to produce ultra clean filtrate since the screen will not shed particles into the filtrate. Each apparatus is supplied with stainless steel support screen, glass support base, 000 ml funnel, anodized aluminum clamp and filtration flask. Kontes Article No. Description Qty mm Filtration Apparatus with S.S. Support, L Funnel and L Flask () Glass Funnel, 90 mm, 000 ml capacity (2) Anodized Aluminum Clamp, 90 mm () Stainless Steel Support Screen, 90 mm (4) Glass Support Base, 90 mm, standard taper 40/5 outer joint (5) Filtration Flask, 000 ml capacity, standard taper 40/5 inner joint Accessories Flask Cap, standard taper 40/5 outer joint PTFE Joint Sleeve, standard taper 40/5, 6/pkg Safety System Economy Three Hole Cap Solvent Bottle Adapter Eliminates Safety System the - See safety page 6 hazards for complete of aluminum details. foil-wrapped solvent containers. Eliminates the safety hazards of aluminum foil-wrapped solvent containers. This unique Solvent Bottle Adapter is made from PBT and converts ULTRA- WARE GL-45 mobile phase caps to standard 4L solvent bottles for direct connection to your HPLC pump. ULTRA-WARE Economy Three Hole Cap is manufactured from PTFE with a TFE/propylene o-ring and a polypropylene screw collar. System includes Bottom-of-the-Bottle Inlet / Sparge Filter. Kontes Article No. Description Safety System with GL-45 Bottle Adapter

39 2mL and 4mL Autosampler Vials without Closures Choice of clear KG- or amber 20 borosilicate glass Available with or without marking spots Made in the USA Made of the highest quality materials, Kimble Chase vials meet or exceed industry specifications and tolerances. These products are available in a variety of standard sizes and finishes. Vials are made from clear, Type borosilicate, expansion glass or amber, Type, borosilicate, 5 expansion glass for light sensitive applications. Vials are packed 00 per tray. Vials with large opening and/or marking spots are also available. Closures are not included. Clear Vials Catalog Cap. O.D. x Ht. Pack Number (ml) (mm) Finish Qty. Qty. 22C 2 2 x 2 crimp CW 2 2 x 2 crimp with marking spot, graduated CL 2 2 x 2 crimp large opening CLW 2 2 x 2 crimp large opening w/ marking spot x 2 mm Vials 22S 2 2 x 2 screw thread, SN 2 2 x 2 screw thread, SW 2 2 x 2 screw thread, w/ marking spot, grad S 4 5 x 45 screw thread, SW 4 5 x 45 screw thread, -425 w/ marking spot Amber Vials Catalog Cap. O.D. x Ht. Pack Number (ml) (mm) Finish Qty. Qty. 522CA 2 2 x 2 crimp CAW 2 2 x 2 crimp w/ marking spot CLA 2 2 x 2 crimp large opening CLAW 2 2 x 2 crimp large opening w/ marking spot SA 2 2 x 2 screw thread, DPA 2 2 x 2 screw thread, SAW 2 2 x 2 screw thread, w/ marking spot, grad SA 4 5 x 45 screw thread, SAW 4 5 x 45 screw thread, -425 w/ marking spot x 45 mm Vials Aluminum Seals, with PTFE-Faced Silicone Rubber Liners Good for resealability, core resistance, multiple injections, and easy penetration Thickness, Fits GPI Thickness, Inches Catalog Aluminum Seal Inches Silicone Number Finish Color PTFE Rubber Qty. N7824- natural N7824 Series Aluminum Seals, with PTFE-Faced Red Rubber Septa Most popular and economical choice for general GC applications Moderate resealability Not recommended for multiple injections or long-term storage Thickness, Fits GPI Thickness, Inches Catalog Aluminum Seal Inches Red Number Finish Color PTFE Rubber Qty. N7826- red N7826 Series

40 Kimble Chase caps are manufactured using the highest quality raw materials and designed for an ideal fit with our autosampler vials. Open-Top Polypropylene Screw Thread Closures with Red PTFE-Faced Silicone Septa Convenient assembly of caps and liners Available with standard or pre-slit septa Autoclavable and used for multiple injections. Catalog GPI Thread Shelf-Pack Number Finish Septa Color Qty. Qty. 78BK 78WH 782BK standard black BK pre-slit black WH standard white WH pre-slit white BK standard black BK pre-slit black BL standard blue BL pre-slit blue BK standard black BK pre-slit black WH standard white WH pre-slit white BK Open-Top Polypropylene Screw Thread Closures with Bonded White PTFE Liners Intended for single-use injection Catalog GPI Thread Number Finish Color Qty. 784WH white BL blue BK black Polypropylene Rack for 2 mm OD Vials Disposable polypropylene vial rack is lettered and numbered for indexing individual vials. Corners interlock for convenient stacking. Catalog Number Hole Diameter x Depth (mm) Qty x 5 0 High Recovery Vials Made to Your Specifications Accuracy Reliability Quality Affordability We can manufacture any vial you can dream up. Flat Exterior Bottoms Ideal for bar coding Sized to fit any tray design Inherently stable Conical Interior Bottoms Maximize your sample recovery Minimizes the use of costly samples Designed based on your needle type Finishes Aluminum seal Screw thread Plug style Available Value Added Services Permanent sample ID (PSID) Fused on or laser etched labels All symbologies - D, 2D Universal readability Pre-labeled and pre-tared Pre-weighed with documentation

41 SPE Disk Glassware and Manifolds All SPE Manifolds are supplied with three-way valves that allow the venting to atmosphere of each station without affecting the vacuum in the rest of the system. SPE Manifolds are manufactured from corrosion resistant stainless steel. Valves have stainless steel bodies with PTFE plugs. The SPE Funnels have a sharp edge on the bottom inside diameter that, combined with the stronger spring in our aluminum clamps, minimizes any horizontal capillary action of the sample and solvent into the disk. Extracted samples can be collected directly into K-D concentrator tubes, EPA water analysis vials, sample vials and test tubes. PTFE-coated stainless steel support screen reduces the possibility of sample-to-sample cross contamination compared to the traditional glass frit. The PTFE coating also protects the support screen from corrosion caused by acid preserved samples. The SPE Support Bases have a specially designed internal surface that reduces dead volume and minimizes holdback of the extracted analyte. Get the most out of using M Empore TM Extraction Disks with our line of SPE Glassware and Manifolds They have been specially designed for use with solid phase extraction disks to give you the highest possible recoveries of extracted analytes from your drinking water, surface water and waste water samples. Using Kontes Brand SPE Glassware with M Empore extraction disks saves you time, saves you money and helps save our environment. M Empore TM is a trademark of M Company. Kontes Complete Article No. Description Qty SPE Manifold Set, 47 mm, -place set (one 47 mm Glassware Set and one -place Manifold) SPE Manifold Set, 47 mm, -place set (three 47 mm Glassware Sets and one -place Manifold) SPE Manifold Set, 47 mm, 6-place set (six 47 mm Glassware Sets and one 6-place Manifold) Replacement Components SPE Glassware Set, 47 mm (Funnel, Support Base, Support Screen, PTFE Gaskets & Clamp) SPE Glassware Set, 90 mm (Funnel, Support Base, Support Screen, & Clamp) SPE Funnel, 47 mm, 00 ml capacity SPE Funnel, 90 mm, 000 ml capacity SPE Support Base, 47 mm SPE Support Base, 90 mm SPE Support Screen, 47 mm, PTFE-coated S.S SPE Support Screen, 90 mm, PTFE-coated S.S Anodized Aluminum Clamp, 47 mm Anodized Aluminum Clamp, 90 mm PTFE Gasket, 47 mm, 25/package (5 included with Glassware Set) Valve Plug SPE Stainless Steel Manifold, -place SPE Stainless Steel Manifold, -place SPE Stainless Steel Manifold, 6-place Accessories SPE Funnel, 47 mm, 000 ml capacity SPE Support Screen, 47 mm, Kel-F SPE Support Screen, 90 mm, Kel-F PTFE Joint Sleeves, for standard taper 40/5 joint, 6/package Glass Cap for standard taper 40/5 inner joint of Manifolds Diaphragm Vacuum Pump, 0/5 VAC, 50/60 Hz

42 INDEX ( to ) Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg C CL CLW CW S SN SW S ,4 545SW ,4, ,4, , ,4, ,4, , , , ,

43 INDEX ( to N7826-) Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg Catalog No. Pg BK BK H BK H BK N BK N BK BL BL WH WH BK BK BK BL WH WH BK BL WH CA CAW CLA CLAW DPA SA SAW SA SAW

44 We re here to help. If you don t find what you need, please call for assistance. To order, contact your local distributor or the office listed below. Customer Service Hours: 8 am - 5 pm EST For additional product, technical, or distributor information, please visit or call Customers outside the United States, please call: Ext. or contact your local Kimble Chase sales office listed below. NORTH AMERICA EUROPE LATIN AMERICA ASIA / PACIFIC Tel: Tel: Tel: Tel: ,.5509,.956 Fax: Fax: Fax: Fax: Cardiff Valley Road, Rockwood, TN 7854 USA Chromaflex, Disposaflex, Flex-Column and Ultra-ware are registered trademarks of Kimble Kontes Asset Management Inc. Microcaps is a registered trademark of Drummond Scientific Company. Bottom-of-the-Bottle is a trademark of Upchurch Scientific. PEEK is a trademark of Victrex plc. All trademarks are the property of their respective owners KC-40

2 CHROMATOGRAPHY. Specifications. t Go To TOC jacketed column shown with optional flow adapter.

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