Leica TCS SPE. Spectacular Imaging! Technical Documentation

Transcription

1 Leica TCS SPE Spectacular Imaging! Technical Documentation

2 Leica TCS SPE Spectacular Imaging Easy to Achieve A Reliable System Affordable Excellence The high resolution spectral confocal Leica TCS SPE is an integrated system, optimized for target applications in small research groups and multi-user environments. A reliable partner providing spectacular results easily and yet affordably.

3 A Research microscope A Inverted B Upright Confocal scan head 3 Supply unit, including three or four lasers and power supply, PC Galvometer stage Microscope control unit 6 Camera port Digital camera EL6000 fluorescence illumination control 9 Smart move microscope control 0 Computer table Microscope table Computer mouse 3 Keyboard Control monitor 3 6 B

4 Specifications Scan Head Scanner Method true confocal Confocal channels Scanner Galvo, [x,y] Sequential scan Channels -, sequential multiplexing Resolution Range (min - max) [pixel] - 0 Scan formats [pixel], 6,, 0, 0 Image depth [bit] or, switchable Spectral Detection Spectral detection Type continuously variable Spectral resolution [nm] nm Bandwidth [nm] 30-0 Detector Detector Detector type ultra high dynamic PMT Detector connection direct Illumination laser Pinhole Pinhole type motorized, variable Range (min - max) [µm] Pinhole adjustment [%] 0-00 Control automated via GUI Beam Splitter Type new high performance dichroics Beam splitter wavelength [nm] 0/3, /633 ND-splitter for TLD [%] 30/0 Beam splitter exchange automated Zoom Zoom type continuously variable Zoom range x - 6x Zoom increment 0. Scan Modes D: x-y x-z x-t 3D: x-y-z x-y-t x-y-λ Band D: x-y-z-t FOV Field of view (diagonal) [mm]. Speed Speed mode uni-directional Line speed [Hz] 00 Line speed range [Hz] 00, 600, 00 [f/s] 3.0 [f/s].0 0 [f/s] 0.36

5 Supply Unit Laser Laser type solid state Laser max Laser excitation wavelength [nm] 0,, 3, 63 Excitation attenuation AOTF Excitation attenuation control automated Range [%] 0-00 Computer Integrated PC Processor Intel Pentium-M HD-Size [GB] 0 Operating system XP-embedded Interfaces Ethernet USB FireWire Parallel Serial Mouse Type optical Monitor Monitor resolution [Pixel] 0 x 0 Monitor size 9 Power Supply Power supply integration Type autoselect Voltage range [V] 00-0 Z-Drive Z-focus galvanometer stage Z-resolution 0 nm Z-focus device, other no Microscope Types upright DM00 CSQ DM00 CSQ inverted DMI 000 CSQ Software Export Formats LEI, LIF, TIFF, AVI, JPEG Modules Acquisition 3D-visualisation Acqusition, time series Averaging Time lapse Multi point time lapse () Colocalization Environment Ratings Humidity [%] 0-0 Operating temperature [ C] -30 Guaranteed stability 3 C +/- C

6 Shutter Itensity Shuter Power System Overview Leica TCS SPE TCS SPE VIS with HC-Optics Solid State Laser: R: SS mw 63 nm G: SS 0 mw 3 nm B: SS 0 mw nm Scan Head Standard Accessories: Fast Z-Movement SuperZ Galvo Inserts Fluo-Illuminat V: SS mw 0 nm SuperZ Galvo Focus SPE 0 EL6000 Supply Unit with integrated PC RGB Insert for SuperZ, standard 3h Solid State Laser AOTF 3 Microscopes: DM00 CSQ VIS Lasersafe 6x fluo BF DM00 CSQ VIS Lasersafe x fluo BF DMI000 CSQ VIS Lasersafe 6x fluo BF TCS SPE w. DM00 CSQ VIS TCS SPE w. DM00 CSQ VIS TCS SPE w. DMI000 CSQ VIS Additional Options: Transmitted Light Detector- Kits TLD for DM TLD for DMI 93 Optical outfit DIC for DM00 CSQ 93 Optical outfit DIC for DM00 CSQ 933 Optical outfit DIC for DMI000 CSQ Motorized xy-stage Kits DM Basic 3-Plate stage motorized kit DMI Basic 3-Plate s tage motorized kit Digital Camera Kit Leica DFC30 FX incl. C-Mount Adapter x 0,63 () 9 Climate Chamber Kit Climate chamber for DMI000 CSQ () Including Heating control unit Including Temp control unit Note: () Available after May 006 6

7 TCS SPE V-VIS with ACS-Optics Scan Head ion: Input / Output Equipment External light source HXP0 burner Liquid light guide Adapter TFT Monitor 9" Keyboard, Mouse Supply Unit with integrated PC R GBV 3 AOTF Solid State Laser DM00 CSQ V-VIS Lasersafe 6x fluo BF DM00 CSQ V-VIS Lasersafe x fluo BF DMI000 CSQ V-VIS Lasersafe 6x fluo BF TCS SPE w. DM00 CSQ V-VIS TCS SPE w. DM00 CSQ V-VIS TCS SPE w. DMI000 CSQ V-VIS SuperZ Galvo Inserts Tables Software LAS AF TCS SPE 0 93 Insert for SuperZ, universal Insert for SuperZ, rotatable Microscope table for upright and inverted systems, passive LAS AF SPE Live Data Mode LAS AF SPE Colocalization 60 0 LAS AF SPE Deconvolution Option Insert for SuperZ Galvo Focus w/o heating LAS AF SPE 3D Visualization 60 0 LAS AF SPE Motion Spy Option CO cover for insert for SuperZ Galvo Focus 60 0 LAS AF SPE Dye Finder Computer table

8 visible radiation: Room Requirements Power Supply Power supply integration Type autoselect Voltage range [V] 00-0 Power consumption [VA] 00 Independent circuits [no.] Frequency [Hz] 0/60 Fuse: standard [A] 0 Environment Humidity (noncondensing) [%] 0-0 Operating temperature [ C] - 30 Guaranteed stability 3 C +/- C Load Capacity and Weight Confocal unit, max [kg] Microscope, max [kg] System [kg] 0 Static floor load [kg/m ] 0 00 mm 0 mm 0mm 00 mm 0 mm 00 mm 00 mm 00 mm 900 mm 900 mm Copyright Leica Microsystems CMS GmbH Am Friedensplatz 3 66 Mannheim Germany 006 Tel. +9 (0)6-0 0 Fax +9 (0)6-0 0 LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. Printed on chlorine-free bleached paper. II/06/SK 00 mm 0mm 00 mm 0 mm 00 mm 00 mm