INFINITY-CORRECTED TUBE LENSES

Transcription

1 INFINITY-CORRECTED TUBE LENSES For use with Infinity-Corrected Objectives Available in Focal Lengths Used by Thorlabs, Nikon, Leica, Olympus, and Zeiss Designs for Widefield and Laser Scanning Applications TTL200 Widefield Tube Lens,, TTL180-A Widefield Tube Lens, f = 180 mm, TTL200MP Laser Scanning Tube Lens,, TL200-2P2 Laser Scanning Tube Lens,, OVERVIEW Features Tube Lenses for Widefield Imaging True Imaging Lenses for Forming a Well-Corrected Infinity Optical System Apochromatically Corrected for Axial Chromatic Aberration Across Field of View Better Overall Aberration Correction than Standard Achromats AR Coatings for High Transmission Through Visible and NIR Wavelength Ranges 200 mm, 180 mm, 165 mm, or 100 mm Focal Length External or External M38 x 0.5 Threading Tube Lenses for Laser Scanning and Widefield Imaging Infinity-Corrected Design Quick Links to Tube Lenses Widefield Laser Scanning Webpage Features f = 180 mm f = 165 mm f = 100 mm Zemax black box files (both directions) for all tube lenses except ITL200 can be accessed by clicking this icon below. Forms a Telecentric System When Paired with an SL50 Scan Lens , , , or AR Coating 200 mm Focal Length Internal and External Threading Compatible with Thorlabs, Nikon, Leica, Olympus, and Zeiss Objectives Diffraction-Limited Optical Performance These infinity-corrected tube lenses are designed for use with infinity-corrected objectives from all major manufacturers, including Dry, Oil Immersion, and Physiology microscope objectives sold by Thorlabs. Designed for high-resolution imaging, biomedical, machine vision, and laser scanning applications, se lenses can be aligned in pairs to create 1-to-1 relays, combined with objectives to create different effective magnification ratios at a scientific camera, used as drop-in replacements for tube lenses in existing systems, or integrated into DIY Cerna Microscopes and or home-built microscopy setups to generate high-quality images. Objective, Scan, and Tube Lens Selection Guide Objectives Microscopy Objectives, Dry Microscopy Objectives, Oil Immersion Physiology Objectives, Water Dipping or Immersion Long Working Distance Objectives Reflective Microscopy Objectives UV Microscopy Objectives 532 and 1064 Objectives Scan Lenses and Tube Lenses Scan Lenses Infinity-Corrected Tube Lenses Click To Enlarge Click Here for Data This graph represents measured axial color data for on-axis rays (0 AOI) focused by a randomly sampled TTL200 widefield tube lens. The TTL200 falls within range of diffraction-limited performance, indicated by shaded pink box, throughout visible range. Additional performance data is available in Specs tab.

2 Standard Widefield Tube Lenses Our widefield tube lenses provide diffraction-limited axial color performance in visible and NIR wavelength ranges. Their effective focal lengths correspond to design focal lengths of popular objectives (see Magnification & FOV tab for details). They are AR coated for high transmission at visible and NIR wavelengths; transmission plots are provided in tables below. The TTL200 series of tube lenses are specifically designed to offer a wider diffraction-limited axial color range than ITL200 tube lens. Specifications for all lenses can be found on Specs tab, as well as Zemax black box files for all lenses except ITL200. An objective lens creates an image of an object at infinity; put anor way, objective collimates light emitted from different positions at object plane. These tube lenses are designed to refocus collimated light rays into an image on active area of a detector, as illustrated in diagram at top of page. In diagram, blue rays originate from object plane at center of optical axis, while red rays originate off-axis. If tube lens is too close, image may suffer from aberrations; if it is too far, vignetting will occur. Laser Scanning Microscopy Tube Lenses The TTL200MP, TL200-CLS2, TL200-2P2, and TL200-3P tube lenses are optimized for laser scanning applications, such as confocal laser scanning, twophoton microscopy, and three-photon microscopy. These lenses are designed to be telecentric when paired with our SL50-CLS2, SL50-2P2, and SL50-3P scan lenses, for use in point-by-point galvo scanning of object plane. These lenses can also be used for widefield imaging over ir specified wavelength ranges. Our standard widefield tube lenses can also be used for laser scanning modalities such as confocal and two-photon microscopy. For example, y can be paired with CLS-SL Visible Scan Lens. Please note that using a standard tube lens in a scanning configuration will limit unvignetted field size, since tube lens must be placed at telecentric pupil distance from objective (e.g., 250 mm for TTL200 lens), which is farr than specified pupil distance of tube lens. Microscope and Objective Compatibility Microscope manufacturers design ir systems with one of several standard tube lens focal lengths, including 200 mm (typical for Thorlabs, Nikon, and Leica microscopes), 180 mm (typical for Olympus microscopes), and 165 mm (typical for Zeiss microscopes). We offer infinity-corrected tube lenses in all of se focal lengths so that home-built microscope systems may make use of se industry standards. In addition, some of our 200 mm focal length tube lenses (Item #'s TTL200 and ITL200) have external M38 x 0.5 threads that allow m to serve as drop-in replacements in Thorlabs and Nikon microscopes. Similarly, microscope objectives are designed to provide magnification engraved on housing when y are used with a tube lens of a specific focal length. The objectives that we offer from Thorlabs, Nikon, and Mitutoyo are all designed to work with 200 mm focal length tube lenses, making m well matched to TTL200 series and ITL200, while objectives that we offer from Olympus are designed for a 180 mm tube lens focal length, making m well matched to TTL180-A. Alternatively, objectives and tube lenses of different design focal lengths may be combined to create different magnification ratios at camera without compromising axial color correction. To calculate system magnification for different tube lens and objective combinations, see Magnification & FOV tab. SPECS Tube Lenses for Widefield Imaging Item # TTL200 TTL200-A TTL200-B TTL200-S8 ITL200 TTL180-A TTL165-A TTL100-A Effective Focal Length 200 mm ± 1% 200 mm 180 mm ± 1% 165 mm ± 1% 100 mm ± 1% Working Distance a,b 148 mm 148 mm 130 mm 118 mm 60 mm Pupil Distance c mm mm mm mm mm Field Size at Image Plane d Ø22 mm Not Available Ø22 mm Ø22 mm Ø15 mm Entrance Pupil Ø20 mm Not Available Ø18 mm Ø16 mm Ø14 mm Lens Design Apochromatic Apochromatic Apochromatic Apochromatic Apochromatic Design Wavelength Range b AR Coating Range to Broadband Single-Layer MgF 2 Coating Visible Wavelengths Visible Wavelengths 400 to to to Axial Color Diffraction Limited Not Available Diffraction Limited Resolution Diffraction Limited f Not Available Diffraction Limited e Surface Quality Scratch-Dig Not Available Scratch-Dig External Threading M38 x 0.5 Bottom Only M38 x 0.5 Bottom Only

3 Housing Length 28.0 mm 28.0 mm 33.5 mm 30.9 mm 31.1 mm Performance Data (Click for Graph) Transmission Axial Color RMS Wavefront Error MTF Distortion Not Available Not Available Not Available Not Available Data Excel Spreadsheet Excel Spreadsheet Excel Spreadsheet Excel Spreadsheet Excel Spreadsheet Zemax Black Box Files Not Available Measured from top edge of housing to image plane (see diagram below). The working distance is valid over design wavelength range, and lens will need to be refocused when used at or wavelengths. To adjust working distance for operation at different wavelengths, use axial color plots and performance data. This is optimal distance between tube lens and entrance pupil of objective (see diagram below). TTL series lenses will provide an unvignetted field up to this diameter so long as lens is focused at wavelength of interest, which may be anywhere from lower end of design wavelength range up to For reference, a Ø22 mm field size is large enough to fill a 4/3" format camera sensor. Appropriate for use with camera pixel sizes down to 2 µm. Click To Enlarge This tube lens schematic above shows working distance and pupil distance for TTL series and ITL200 tube lenses. The working distance corresponds to distance from top surface of housing to image plane. The pupil distance, defined as distance between bottom edge of tube lens housing and entrance pupil of objective, can be set anywhere within range specified in table above, since rays from objective are in parallel bundles. If tube lens is too close, image may suffer from aberrations; if it is too far, vignetting will occur. Lenses with external threads are engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space), as shown in diagram above. Item #'s TTL200 and ITL200

4 should be inserted with M38 x 0.5 threading facing objective. Tube Lenses for Laser Scanning and Widefield Imaging Item # TTL200MP TL200-CLS2 TL200-2P2 TL200-3P Design Wavelength Range AR Coating Range Effective Focal Length mm 200 mm 200 mm 200 mm Working Distance a 148 mm mm mm mm Pupil 228 mm Distance b (Telecentric) mm (Telecentric) +24/-5 mm mm (Telecentric) +24/-5 mm mm (Telecentric) +24/-5 mm Exit Pupil Diameter 25 mm (Maximum) c 20 mm (Maximum) 20 mm (Maximum) 20 mm (Maximum) f/# Field of View (Diffraction Limited) Clear Aperture ±11 mm 16.3 mm x 16.3 mm (FN23) for mm x 14.1 mm (FN20) at mm x 7.8 mm (FN11) at mm x 15.5 mm (FN22) for mm x 15.5 mm (FN22) for mm x 12.4 mm (FN17.6) at 1900 Ø36.8 mm Ø47.0 mm Ø47.0 mm Ø47.0 mm Lens Design Apochromatic Apochromatic Apochromatic Apochromatic Axial Color F-Theta Distortion Threading Housing Length Diffraction Limited Diffraction Limited Diffraction Limited Diffraction Limited <0.2% <0.3% <0.3% <0.3% External Threads (Top and Bottom) Performance Data (Click for Graph) Transmission Axial Color RMS Wavefront Error MTF Internal Threads on Top External Threads on Bottom 41.6 mm Data Zemax Black Box Files Excel Spreadsheet Excel Spreadsheet Excel Spreadsheet Excel Spreadsheet Measured from housing edge to intermediate image plane (see diagram below). This is optimal distance between lens and objective (see diagram below). Diffraction-Limited Up to Ø20 mm.

5 The tube and scan lens schematic above shows SL50-2P2 scan lens and TL200-2P2 tube lens. It indicates working distance and pupil distance for tube lens, as well as location of scan lens and scan plane. Most of se values also apply for TL200-CLS2 and TL200-3P with ir corresponding scan lenses. Note that for SL50-CLS2 entrance pupil at scan plane is a maximum of Ø4 mm and pupil distance tolerance of scan lens is +12/-5 mm when used with TL200-CLS2. These lenses are engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space). MAGNIFICATION & FOV Magnification and Sample Area Calculations Magnification The magnification of a system is multiplicative product of magnification of each optical element in system. Optical elements that produce magnification include objectives, camera tubes, and trinocular eyepieces, as shown in drawing to right. It is important to note that magnification quoted in se products' specifications is usually only valid when all optical elements are made by same manufacturer. If this is not case, n magnification of system can still be calculated, but an effective objective magnification should be calculated first, as described below. To adapt examples shown here to your own microscope, please use our Magnification and FOV Calculator, which is available for download by clicking on red button above. Note calculator is an Excel spreadsheet that uses macros. In order to use calculator, macros must be enabled. To enable macros, click "Enable Content" button in yellow message bar upon opening file. Example 1: Camera Magnification When imaging a sample with a camera, image is magnified by objective and camera tube. If using a 20X Nikon objective and a 0.75X Nikon camera tube, n image at camera has 20X 0.75X = 15X magnification. Example 2: Trinocular Magnification When imaging a sample through trinoculars, image is magnified by objective and eyepieces in trinoculars. If using a 20X Nikon objective and Nikon trinoculars with 10X eyepieces, n image at eyepieces has 20X 10X = 200X magnification. Note that image at eyepieces does not pass through camera tube, as shown by drawing to right. Using an Objective with a Microscope from a Different Manufacturer Magnification is not a fundamental value: it is a derived value, calculated by assuming a specific tube lens focal length. Each microscope manufacturer has adopted a different focal length for ir tube lens, as shown by table to right. Hence, when combining optical elements from different manufacturers, it is necessary to calculate an effective magnification for objective, which is n used to calculate magnification of system. When viewing an image with a camera, system magnification is product of objective and camera tube magnifications. When viewing an image with trinoculars, system magnification is product of objective and eyepiece magnifications. Manufacturer Leica Mitutoyo Nikon Olympus Thorlabs Zeiss Tube Lens Focal Length f = 180 mm f = 165 mm The rows highlighted in green denote manufacturers that do not use tube lenses. The effective magnification of an objective is given by Equation 1:

6 (Eq. 1) Here, Design Magnification is magnification printed on objective, f Tube Lens in Microscope is focal length of tube lens in microscope you are using, and f Design Tube Lens of Objective is tube lens focal length that objective manufacturer used to calculate Design Magnification. These focal lengths are given by table to right. Note that Leica, Mitutoyo, Nikon, and Thorlabs use same tube lens focal length; if combining elements from any of se manufacturers, no conversion is needed. Once effective objective magnification is calculated, magnification of system can be calculated as before. Example 3: Trinocular Magnification (Different Manufacturers) When imaging a sample through trinoculars, image is magnified by objective and eyepieces in trinoculars. This example will use a 20X Olympus objective and Nikon trinoculars with 10X eyepieces. Following Equation 1 and table to right, we calculate effective magnification of an Olympus objective in a Nikon microscope: The effective magnification of Olympus objective is 22.2X and trinoculars have 10X eyepieces, so image at eyepieces has 22.2X 10X = 222X magnification. Sample Area When Imaged on a Camera When imaging a sample with a camera, dimensions of sample area are determined by dimensions of camera sensor and system magnification, as shown by Equation 2. (Eq. 2) The camera sensor dimensions can be obtained from manufacturer, while system magnification is multiplicative product of objective magnification and camera tube magnification (see Example 1). If needed, objective magnification can be adjusted as shown in Example 3. As magnification increases, resolution improves, but field of view also decreases. The dependence of field of view on magnification is shown in schematic to right. Example 4: Sample Area The dimensions of camera sensor in Thorlabs' 1501M-USB Scientific Camera are 8.98 mm 6.71 mm. If this camera is used with Nikon objective and trinoculars from Example 1, which have a system magnification of 15X, n image area is: Sample Area Examples The images of a mouse kidney below were all acquired using same objective and same camera. However, camera tubes used were different. Read from left to right, y demonstrate that decreasing camera tube magnification enlarges field of view at expense of size of details in image. Acquired with 1X Camera Tube (Item # WFA4100) Acquired with 0.75X Camera Tube (Item # WFA4101) Acquired with 0.5X Camera Tube (Item # WFA4102) Tube Lenses for Widefield Imaging 200 mm Effective Focal Length Used by Thorlabs, Nikon, Leica, and Mitutoyo Four AR Coatings Available: TTL200 and TTL200-A: TTL200-B: TTL200-S8: Broadband MgF 2 Coating ITL200: Visible Wavelengths

7 148 mm Working Distance over Design Wavelength Range of Thorlabs offers five infinity-corrected tube lenses for widefield imaging. All TTL200 series lenses are designed with diffraction-limited axial color correction over and over These lenses can provide diffraction-limited performance about any target wavelength from 400 to 2000, provided that tube lens is set to focus on camera at that target wavelength. These lenses' effective focal length of 200 mm is design focal length used by Thorlabs, Nikon, Leica, and Mitutoyo objectives. Please note that using se lenses for laser scanning will result in vignetting and uneven spot sizes over FOV; for integration into a telecentric laser scanning system, see tube lenses below. The TTL200 and TTL200-A are AR coated for visible wavelength range ( ), offering improved performance at shorter wavelengths (<480 ) to accommodate applications using 405 and 443 illumination. Note that while AR coating is designed for , glass used in TTL200 is highly absorptive below 400 and transmission will fall off sharply. The TTL200-B is AR coated for NIR wavelength range ( ), making it ideal for NIR fluorescence and NIR DIC imaging. The TTL200-S8 utilizes a broadband MgF 2 single-layer coating with a low transmission roll-off throughout visible and NIR, with peak transmission centered at 830. See graph to right for a comparison of all 200 mm tube lenses, and Specs tab for additional performance data. TTL200 series lenses can be custom coated with eir a single layer or a multi-layer AR coating optimized for transmission over a user-specified wavelength range; contact Tech Support for details. Click Here for: TTL200 Series Data ITL200 Data Typical transmission of five tube lenses designed for widefield imaging. Additional performance data is in Specs tab. TTL200 ITL200 TTL200-A TTL200-B TTL200-S8 External Threadings M38 x 0.5 Bottom Only M38 x 0.5 Bottom Only The TTL200-A, TTL200-B, and TTL200-S8 tube lenses are engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space). Mounting Options The TTL200-A, TTL200-B and TTL200-S8 feature external (2.035"-40) threading on both sides that connects to our Ø2" lens tubes and many elements of our 60 mm Cage System. To integrate one of se tube lenses with Thorlabs' Cerna DIY Microscopy Systems, use M05 lens tube to connect to WFA4111 Dovetail Adapter (available below). The TTL200 and ITL200 use external M38 x 0.5 threading. This threading can be converted to external (2.035"-40) threading using A20 adapter available below. Alternatively, WFA4111 Dovetail Adapter, also available below, directly accepts a TTL200 or ITL200 tube lens and can integrate it with a Cerna microscope. We also offer WFA4110 Dovetail Adapter, which is a version of WFA4111 with TTL200 tube lens built in. TTL200 Tube Lens,, ARC: , External M38 x 0.5 Threads $ Today ITL200 Tube Lens,, External M38 x 0.5 Threads $ Today TTL200-A Customer Inspired!&nbspTube Lens,, ARC: , External Threads $ Today TTL200-B Customer Inspired!&nbspTube Lens,, ARC: , External Threads $ Today TTL200-S8 Customer Inspired!&nbspTube Lens,, Broadband MgF 2 Coating, External Threads $ Today f = 180 mm Tube Lens for Widefield Imaging 180 mm Effective Focal Length Used by Olympus AR Coating for mm Working Distance over Design Wavelength Range of Thorlabs' TTL180-A infinity-corrected tube lens for widefield imaging is designed with diffraction-limited axial color correction over This lens can provide diffraction-limited performance about any target wavelength from 400 to 2000, provided that tube lens is set to focus on detector at that target wavelength. Its effective focal length of 180 mm is design focal length used by Olympus objectives. When this lens is paired with an Olympus objective, magnification of system will be that printed on objective. When this lens is paired with an objective from anor manufacturer, magnification of system can be calculated as explained in Magnification & FOV tab. Please note that using se lenses for laser scanning will result in vignetting and uneven spot sizes over FOV; for integration into a telecentric laser scanning system, see tube lenses below. Click Here for Data Typical transmission of f = 180 mm tube lens designed for widefield imaging. The blue-shaded region indicates design wavelength range. Additional performance data is in Specs tab. The TTL180-A is AR coated for visible wavelength range ( ), offering improved performance at shorter wavelengths (<480 ) to accommodate applications using 405 and 443 illumination. Note that while AR coating is designed for , glass used in TTL180-A is highly absorptive below 400 and transmission will fall off sharply. See graph to right for a transmission measurement, and Specs tab for additional performance data. This lens can be custom coated with eir a single layer or a multi-layer AR coating optimized for transmission over a user-specified

8 wavelength range; contact Tech Support for details. This tube lens is engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space). Mounting Options The TTL180-A features external (2.035"-40) threading on both sides that connects to our Ø2" lens tubes and many elements of our 60 mm Cage System. To integrate one of se tube lenses with Thorlabs' Cerna DIY Microscopy Systems, use M05 lens tube to connect to WFA4111 Dovetail Adapter (available below). TTL180-A Customer Inspired!&nbspTube Lens, f = 180 mm, ARC: , External Threads $ Today f = 165 mm Tube Lens for Widefield Imaging 165 mm Effective Focal Length Used by Zeiss AR Coating for mm Working Distance over Design Wavelength Range of Thorlabs' TTL165-A infinity-corrected tube lens for widefield imaging is designed with diffraction-limited axial color correction over This lens can provide diffraction-limited performance about any target wavelength from 400 to 2000, provided that tube lens is set to focus on detector at that target wavelength. Its effective focal length of 165 mm is design focal length used by Zeiss objectives. When this lens is paired with an Zeiss objective, magnification of system will be that printed on objective. When this lens is paired with an objective from anor manufacturer, magnification of system can be calculated as explained in Magnification & FOV tab. Please note that using se lenses for laser scanning will result in vignetting and uneven spot sizes over FOV; for integration into a telecentric laser scanning system, see tube lenses below. Click Here for Data Typical transmission of f = 165 mm tube lens designed for widefield imaging. The blue-shaded region indicates design wavelength range. Additional performance data is in Specs tab. The TTL165-A is AR coated for visible wavelength range ( ), offering improved performance at shorter wavelengths (<480 ) to accommodate applications using 405 and 443 illumination. Note that while AR coating is designed for , glass used in TTL165-A is highly absorptive below 400 and transmission will fall off sharply. See graph to right for a transmission measurement, and Specs tab for additional performance data. This lens can be custom coated with eir a single layer or a multi-layer AR coating optimized for transmission over a user-specified wavelength range; contact Tech Support for details. This tube lens is engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space). Mounting Options The TTL165-A features external (2.035"-40) threading on both sides that connects to our Ø2" lens tubes and many elements of our 60 mm Cage System. To integrate one of se tube lenses with Thorlabs' Cerna DIY Microscopy Systems, use M05 lens tube to connect to WFA4111 Dovetail Adapter (available below). TTL165-A Customer Inspired!&nbspTube Lens, f = 165 mm, ARC: , External Threads $ Today f = 100 mm Tube Lens for Widefield Imaging 100 mm Effective Focal Length AR Coating for mm Working Distance over Design Wavelength Range of Thorlabs' TTL100-A infinity-corrected tube lens for widefield imaging is designed with diffraction-limited axial color correction over This lens can provide diffraction-limited performance about any target wavelength from 450 to 2000, provided that tube lens is set to focus on detector at that target wavelength. Its effective focal length of 100 mm is half of design focal length used by Thorlabs, Nikon, Leica, and Mitutoyo objectives. When this lens is paired with an objective from one of se manufacturers, magnification of system will be half of that printed on objective, as explained in Magnification & FOV tab. Please note that using se lenses for laser scanning will result in vignetting and uneven spot sizes over FOV; for integration into a telecentric laser scanning system, see tube lenses below. Click Here for Data Typical transmission of f = 100 mm tube lens designed for widefield imaging. The blue-shaded region indicates design wavelength range. Additional performance data is in Specs tab. The TTL100-A is AR coated for visible wavelength range ( ), offering improved performance at shorter wavelengths (<480 ) to accommodate applications using blue illumination. Note that while AR coating is designed for , glass used in TTL100-A is highly absorptive below 450 and transmission will fall off sharply. See graph to right for a transmission measurement, and Specs tab for additional performance data. This lens can be custom coated with eir a single layer or a multi-layer AR coating optimized for transmission over a user-specified wavelength range; contact Tech Support for

9 details. This tube lens is engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space). Mounting Options The TTL100-A features external (2.035"-40) threading on both sides that connects to our Ø2" lens tubes and many elements of our 60 mm Cage System. To integrate one of se tube lenses with Thorlabs' Cerna DIY Microscopy Systems, use M05 lens tube to connect to WFA4111 Dovetail Adapter (available below). TTL100-A Customer Inspired!&nbspTube Lens, f = 100 mm, ARC: , External Threads $ Today Tube Lenses for Laser Scanning and Widefield Imaging 200 mm Effective Focal Length Used by Thorlabs, Nikon, Leica, and Mitutoyo Four AR Coatings Available: TTL200MP: TL200-CLS2: TL200-2P2: TL200-3P: Two Working Distance Options: TTL200MP: 148 mm TL200-CLS2, TL200-2P2, and TL200-3P: mm Thorlabs offers four infinity-corrected tube lenses that have been designed for laser scanning applications. The TTL200MP features diffraction-limited axial color correction and resolution over Click Here for: TTL200MP Data TL200-CLS2 Data TL200-2P2 Data TL200-3P Data Typical transmission of our telecentric tube lenses designed for laser scanning microscopy. Additional performance data is in Specs tab , is AR coated for visible and NIR wavelengths ( ), and can be paired with our SL50-CLS2 ( ), SL50-2P2 ( ), or SL50-3P ( ) scan lenses. The TL200-CLS2 is AR coated for visible and NIR range ( ), making this lens ideal for confocal laser scanning microscopy; it is designed to be used with SL50-CLS2 scan lens. Additionally, broad coating range makes TL200-CLS2 a good choice for applications involving both multiphoton microscopy and visible photoactivation or targeting. In comparison, TL200-2P2 and TL200-3P are AR coated for and , respectively, and are designed for use with SL50-2P2 and SL50-3P scan lenses, respectively. These two lenses are ideal for two-photon or three-photon imaging. These tube lenses are engraved with an arrow next to an infinity symbol ( ) to indicate which side of lens should face objective (infinity space). In general, laser scanning microscopy systems pair a scan lens with a tube lens, such as those sold here, to create an infinity-corrected optical system. In laser scanning systems, a laser beam incident on back aperture (entrance pupil) of scan lens is scanned through a range of angles. This translates position of spot formed in image plane of scan lens across field of view. Telecentric scan lens systems are designed to create a uniform spot size in image plane at every scan position, resulting in an image resolution that varies minimally over entire field of view. See Telecentric Lenses tutorial for additional information. Mounting Options The TTL200MP features external (2.035"-40) threading on both sides that The tube and scan lens schematic above shows SL50-2P2 scan lens and TL200-2P2 tube lens. It indicates working distance and pupil distance for tube lens, as well as location of scan lens and scan plane. For correct distances and plane sizes for each tube lens, please see Specs tab. connects to our Ø2" lens tubes and many elements of our 60 mm Cage System. To integrate this tube lens with Thorlabs' Cerna DIY Microscopy Systems, use M05 lens tube to connect to WFA4111 Dovetail Adapter (available below). The TL200-CLS2, TL200-2P2, and TL200-3P tube lenses feature (2.035"-40) threading; y are externally threaded on end of lens which faces objective and internally threaded on end of lens which faces scan lens. This design allows tube lenses to be connected to Ø2" lens tubes and/or mounted within our 60 mm Cage System. To integrate this tube lens with Thorlabs' Cerna DIY Microscopy Systems, use M05 lens tube to connect to WFA4111 Dovetail Adapter (available below). TTL200MP Laser Scanning Tube Lens,, ARC: $1, Today TL200-CLS2 Laser Scanning Tube Lens,, ARC: $5, Lead Time TL200-2P2 Laser Scanning Tube Lens,, ARC: $5, Lead Time TL200-3P Laser Scanning Tube Lens,, ARC: $5, Lead Time Mechanical Adapters for TTL200 and ITL200 Tube Lenses

10 Easily Integrate Tube Lenses with Thorlabs' Construction Systems WFA4111: D1N Male Dovetail for Using Tube Lenses in Cerna DIY Systems and Microscopes A20: External Threading for Compatibility with Lens Tubes Thorlabs offers two styles of adapters for use with externally M38 x 0.5-threaded TTL200 and ITL200 tube lenses, allowing m to be integrated with Thorlabs' lens tube systems and Cerna DIY Microscopy Platform. Our or tube lenses already have external (2.035"-40) threads. The WFA4111 adapter allows M38 x 0.5-threaded tube lenses to be easily integrated with Cerna microscopes and -threaded components. A WFA4111 can be used to integrate a TTL200 tube lens into a custom epi-illumination module on a DIY Cerna Microscope. Our WFA4111 Dovetail Adapter directly accepts TTL200 and ITL200. Alternatively, external threads on top of adapter allow externally - threaded lenses to be connected via an M05 lens tube. The bottom of adapter features a male D1N dovetail, making it compatible with our DIY Cerna systems. The threads on top can also be used to integrate user-designed camera tubes constructed from -threaded lens tubes. The A20 allows TTL200 and ITL200 to be easily converted to threading, enabling construction of an optical system consisting of a scan lens and a tube lens using Thorlabs' standard lens tube components and -threaded GCM102(/M) 2D galvo mounting adapter. We also offer -threaded adapters for common objective threads. The SM38RR retaining ring can be used to lock tube lens in place when using eir adapter. We also offer WFA4110 Dovetail Adapter, a version of WFA4111 adapter that includes TTL200 tube lens. WFA4111 Adapter with Male D1N Dovetail, External Threads, and Internal M38 x 0.5 Threads $ Today A20 Adapter with External Threads and Internal M38 x 0.5 Threads $47.50 Today SM38RR Customer Inspired!&nbspM38 x 0.5 Retaining Ring $12.75 Today