MVX-7100 µl Workstation

Transcription

1 MVX-7100 µl Workstation Operator s Manual Manual Part Number Rev 1

2 COPYRIGHT Teledyne Technologies Incorporated. All rights reserved Rev 1, April, 2017 Teledyne CETAC Technologies authorizes its customers to reproduce, transmit, or store this document in its entirety, including this page, for the express purpose of installing, operating, or maintaining the product described herein. Teledyne CETAC Technologies Customer Service & Support Industrial Road Omaha, Nebraska 68144, USA Phone (800) (USA only) Phone (402) Fax (402) REVISIONS Teledyne CETAC Technologies strives to provide the scientific community with an unparalleled combination of effective technology and continuing value. Modular upgrades for existing instruments will continue to be a prime consideration as designs progress. Teledyne CETAC Technologies reserves the right to revise this document and/or improve products described herein at any time without notice or obligation. Warranty registration entitles the named owner exclusively to manual change pages/new editions as they are published. TRADEMARK ACKNOWLEDGEMENTS Windows is a registered trademark of Microsoft Corporation in the United States and other countries. Teflon is a trademark or registered trademark of E.I. du Pont de Nemours and Company. Tygon is a registered trademark of Saint- Gobain Performance Plastics. All other marks are the property of their respective owners. LICENSE ACKNOWLEDGEMENTS Autosampler firmware compiled using FreeRTOS Version

3 Contents About This Manual... 6 Who Should Read This Manual?... 6 Where to Go for More Information Introduction to the MVX-7100 µl Workstation...9 Overview... 9 What does the MVX-7100 µl workstation do?... 9 How does the MVX-7100 µl workstation work?... 9 How is the MVX-7100 µl workstation controlled?...10 Workstation Components...10 Additional Equipment Required...12 Optional Accessories...13 Intended Use...14 Chemical Compatibility...14 Non-Wetted Components...14 Wetted Components Preparing for Installation Choosing a Configuration...17 Choosing a Location for Installation...18 Proximity to the ICP-MS Instrumentation...18 Space Requirements...19 Work Surface Requirements...19 Rinse Solution/Liquid Waste Requirements...19 Power Requirements...20 Unpacking the MVX-7100 µl Workstation Installing the Workstation Tools Required for Installation...23 Overview of Installation Tasks...23 Installing the Software...24 Installing the CETAC Workstation Software...24 Installing the Agilent MassHunter Workstation Software...25 Installing the Accessory Module Mounting Blocks...25 Mounting the SPM and VPM Accessory Modules...27 Connecting the Power Supply...27 Connecting the MVX-7100 µl Workstation to the Host PC...29 Connecting a USB Cable...30 Connecting a Serial Cable...31 Installing the VSM and SPM Syringes...32 Installing and Aligning a Sample Needle...34 Tools Required...35 Installing the Sample Needle

4 Contents Adjusting the Sample Needle Height Testing the New Needle Aligning the Sample Needle...38 Installing Fittings on the Tubing...38 Connecting Tubing to the VSM and SPM...39 Connecting the VPM to an ICP-MS Nebulizer...42 Connecting Tubing to the Rinse Station...42 Direction of Flow Rinse Solution Recycling Gravity Drain Arrangement Pumped Drain Arrangement Tubing Considerations Connecting the Tubing to the Peristaltic Pump Installing the Sample Rack Base or the Optional PRM Module...45 Sample Rack Base Peltier Rack Module Verifying Installation...47 Verifying Communication with the MVX-7100 µl Workstation...47 How to Tell If There Is a Communication Problem Checking the Sample Needle/Sample Tube or Well Alignment Using the Workstation...51 Establishing Optimal Operating Conditions...51 Creating the Lab Environment Purchasing Supplies Arranging the Sample Vial Racks...53 Using Septum Vials...53 Starting the Workstation...53 Shutting Down the Workstation...54 Flushing the Rinse Station and Flow Path...54 Calibrating the Z Depth...55 When to calibrate the Z depth How to calibrate the Z depth Writing and Running Scripts with the CETAC Workstation Software Maintaining the Workstation...59 Cleaning the Workstation...59 Daily External Cleaning Weekly Cleaning Checking for Leaks...61 Replacing Peristaltic Pump Tubing...61 Replacing the Sample Needle...63 Tools Required Removing the Old Needle Installing the New Needle Adjusting the Sample Needle Height Testing the New Needle Replacing the Rinse Station Tubing...67 Replacing the Battery...68 Replacing the Fuse Troubleshooting the Workstation

5 Contents Power System Problems...71 Communications Interface Problems...72 RS-232 Cable Problems...72 USB Cable Problems...72 Software Configuration Problems...73 Z-Drive Assembly Problems...74 Returning the Product to CETAC for Service...75 Shipping the Product...75 Product Warranty Statement...75 Returned Product Procedures...76 Returned Product Warranty Determination Operating a CETAC Workstation Using a Terminal Program Using C-Term...79 Installing C-Term...79 Starting C-Term...79 Overview of the C-Term Window...80 Configuring C-Term...80 Autosampler Commands Safety and Regulatory Information Characteristics...83 Environmental Characteristics...83 Electrical Characteristics...84 Safety Notices...87 Power Cord Set Requirements...87 Power Cord Safety Maintenance...87 Mains Disconnect...87 Cleaning Instructions...88 Mechanical Hazards...88 Operating Environment...89 Explanation of Caution and Warning Notices...90 Electromagnetic Interference...90 Explanation of Regulatory Marks Glossary

6 Contents About This Manual This document describes the procedures for installing, using, and maintaining the CETAC MVX-7100 µl workstation, which comprises the main XYZ autosampler unit, the CETAC SPM-700+ syringe pump module, and the CETAC VSM-700 valve syringe module. This document also provides information regarding the design of the workstation and the troubleshooting of minor problems. Who Should Read This Manual? The primary audience for this manual consists of analytical chemists and laboratory technicians. To use this manual effectively, you should have a basic knowledge of chemistry, a basic knowledge of electronic sampling equipment, at least a beginning level of computer experience, and a working knowledge of the analytical instrumentation used with this workstation (typically, but not exclusively, inductively coupled plasma mass spectrometry (ICP-MS) instrumentation). WARNING CHEMICAL INJURY HAZARD The workstation is intended for use only by qualified operators who have been trained in safe laboratory practices. Make sure you know the hazards associated with all of the chemicals you are using, and take the appropriate precautions. Exposure to laboratory chemicals may result in serious injury. Where to Go for More Information In addition to this manual, you can refer to the following resources: The software manual for the instrument you are using The Teledyne CETAC Technologies Website: Teledyne CETAC Technologies Customer Service and Support: 1 (800) (402) (402) (Fax) cetacservice@teledyne.com 6

7 Contents 7

8

9 1 Introduction to the MVX-7100 µl Workstation Overview What does the MVX-7100 µl workstation do? The Teledyne CETAC Technologies MVX-7100 µl workstation provides sample handling (sample preparation and sample introduction capability) for sample volumes down to 5 µl for ICP-MS instrumentation. The syringe-driven sample introduction and subsequent rinse cycles allows for stable low-flow applications with low detection limits. Syringe aliquot control combined with flow injection allows for accurate sample volumes to be handled and superior washout. The MVX-7100 µl workstation utilizes a non-metallic liquid-facing flow path to minimize system background and provide low detection limits. How does the MVX-7100 µl workstation work? By the action of onboard syringe pumps, the MVX-7100 µl workstation uses a quartz septum piercing needle to aspirate and dispense samples and associated media for the purpose of both preparing samples, calibration standards and other solutions in vials, tubes or well plate wells and introducing solutions to ICP-MS instrumentation via a sample loop and switching valve arrangement for analytical measurement. Sample aliquots and dilution/preparation media volumes are accurately controlled by a software operated syringe pump. For sample introduction, sample aliquots are incorporated into a liquid flow path that is continually moving to ICP-MS sample introduction apparatus (typically a nebulizer and spray chamber arrangement). 9

10 Chapter 1: Introduction to the MVX-7100 µl Workstation How is the MVX-7100 µl workstation controlled? The MVX-7100 µl workstation is connected to an ICP-MS host computer via a serial or USB cable. The CETAC Workstation software is installed on the host computer and all workstation commands are handled by this software. For the purpose of analysis, an ICP-MS instrument is typically triggered to act at specified times (be it measure or delay) by the workstation to ensure synchronization between the MVX-7100 automation and the analytical instrument hardware. Triggering may also be set up in the opposite direction, that is, the MVX-7100 µl workstation can receive a trigger signal from an ICP- MS instrument to initiate a specific action. Workstation Components Z-Drive Assembly Autosampler Power Indicator Lamp Syringe Pump Module Valve Syringe Module Arm Rinse Station Sample Vials Sample Vial Racks Reagent Vial Racks and Reagent Vials Sample Well Plates Base FIGURE 1-1 Front View. The following components are located at the front of the workstation: Arm. The XYZ Autosampler arm moves the sample needle horizontally. Power Indicator Lamp. This LED indicates that the XYZ Autosampler component of the workstation is connected to the power supply and that power is being delivered. Reagent Vial Rack and Reagent Vials. This rack holds six 20 ml capacity reagent vials and is adjustable in height with respect to the sample needle. Rinse Station. The dual rinse station is located at the left side of the reagent vials positions at the back of the sample base. Either, or both, positions of the dual rinse station may be used during operation. Sample Rack Base. The sample rack base holds sample racks, 96 well plates and 384 well plates in place for analysis. 10

11 Chapter 1: Introduction to the MVX-7100 µl Workstation Sample Vials. 1.5 ml capacity sample vials may be used with the MVX system as well as micro centrifuge tubes. Sample Vial Racks. These racks hold sample vials or tubes. The number and type of vials or tubes depends on the application in question and on the configuration of the Automation that is required. Sample Well Plates. 96 and 384 position well plates may be used to contain samples for analysis instead of sample vials or tubes. Syringe Pump Module (SPM). The syringe pump module delivers a liquid carrier or reagent flow (with or without a sample incorporated) to an ICP- MS as part of the sample introduction process. One or more (up to 15) CETAC SPM-700+ syringe pump modules can be connected to the MVX µl Workstation as required by specific applications. Valve Syringe Module (VSM). The valve syringe module controls aspiration and dispensation of solutions between vials, tubes or well plate wells as part of sample preparation and controls aspiration of a sample for positioning onto a sample loop prior to introduction to an ICP-MS. Z-Drive Assembly. The Z-Drive assembly moves the sample needle vertically. The assembly includes a Z-Axis motor assembly as well as the sample needle. The Z-Drive motor assembly attaches onto the Autosampler arm. Auxiliary Port Ethernet Port Serial Ports Syringe Pump Module (SPM) Valve/Pump Module (VSM) XYZ Autosampler Power Switch Autosampler Power Supply USB Port VSM Power Switch SPM Power Switch Z-Drive Connector Power Supply Peristaltic Pump Power and Communications Cable Accessory Mount FIGURE 1-2 Rear View. The following components are located at the rear of the workstation: Accessory Mounts. These blocks attach to the XYZ Autosampler and provide a base for either an SPM or VSM module. Auxiliary Port. This optional input/output connector allows single direction or bidirectional triggering between the MVX-7100 system and other instrumentation. 11

12 Chapter 1: Introduction to the MVX-7100 µl Workstation Ethernet Port. The Ethernet port can be used to interface the XYZ Autosampler with the ICP-MS host computer. This port and the associated LEDs are disabled by default. Peristaltic Pump. This peristaltic pump drives the movement of rinse agent to the dual rinse station positioned on the front of the MVX-7100 system as well as the removal of rinse agent via the drain port. Power Supply. This power supply box provides power to the individual XYZ Autosampler, VSM and SPM components of the MVX-7100 µl Workstation. Use a four-port power supply for the XYZ autosampler and up to three accessory modules (such as the VPM, one SPM and the PRM). An eight-channel power supply is available for applications which require two or more SPM modules. Power Supply Power and Communications Cable. These cables connect the Power Supply to the XYZ Autosampler, VSM and SPM components of the MVX-7100 and allow power provision and communications between them. RS-232 Serial I/O Ports (COM1 and COM2). The COM1 RS232 Serial port can be used to interface is the XYZ Autosampler with the ICP-MS host computer. The COM2 RS232 Serial port connects the XYZ Autosampler to the power supply. SPM Power Switch. This switch turns the power to the syringe pump module on and off. USB Port. This USB port can be used to interface the XYZ Autosampler with the ICP-MS host computer. VSM Power Switch. This switch turns the power to the valve syringe module on and off. XYZ Autosampler Power Switch. This switch turns the power to the XYZ Autosampler component on and off. Z-Drive Connector. The Z-drive contains an integral motor and lead screw, and is powered and controlled through this connector on the back of the XYZ Autosampler. Supplied components depend on the application and the exact version of the workstation. See the packing list in the shipping container to see exactly which components are supplied. Additional Equipment Required A Host PC. This computer controls the MVX-7100 µl workstation. This could be the same computer that is used to operate the associated ICP-MS instrumentation. This computer must have a free USB port or RS232 serial port. Such a host PC is not supplied by Teledyne CETAC Technologies with the MVX-7100 µl workstation. Sample Introduction Nebulizer and Spray Chamber Apparatus. A delivery line can be connected between the sample out port of the sixport switching valve of the VPM module and most types of nebulizer for introduction of sample via a spray chamber to an ICP-MS instrument. The 12

13 Chapter 1: Introduction to the MVX-7100 µl Workstation flow rate of sample being pushed by the SPM module syringe is dictated by the MVX-7100 operating software and is matched to the flow rate of the nebulizer in use (such as 50 µl min -1, 100 µl min -1, 200 µl min -1, r 400 µl min -1 ). This makes most nebulizers compatible with the MVX-7100 µl workstation. Optional Accessories Depending on the application, you may also wish to purchase the following items. See the MVX-7100 Accessories and Parts Catalog, available on for part numbers: CETAC SPM-700+ Syringe Pump Module. One or more CETAC SPM-700+ syringe pump modules can be incorporated to the MVX-7100 µl Workstation for applications where additional reagent flows are required to be delivered to an ICP-MS instrument. CETAC Peltier Rack Module (PRM). The PRM module, as shown in Figure 1-3, is a temperature controlled sample rack base that can hold up to two sample racks and well plates at fixed temperatures between 4 C and 40 C. This accessory is provided with its own manual. FIGURE 1-3 Peltier Rack Module Accessory. Rack and Well Plate Options. A number of sample tube/vial racks and well plates are compatible with the MVX-7100 µl workstation: o o o o VT54. These sample vial racks are compatible with HPLC style vials of 1.5 ml capacity. SR48. These sample tube racks are compatible with micro centrifuge tubes. 96 Well Plates. The MVX-7100 is compatible with most standard 96 well plates of varying well capacity and up to 44 mm in height. Teledyne CETAC Technologies can provide 96 well plates. 384 Well Plates. The MVX-7100 is compatible with most standard 384 well plates of varying well capacity. Teledyne CETAC Technologies can provide 384 well plates. Pierceable Films. Pierceable films can be used to provide a top layer seal over a 96 or 384 well plate to inhibit evaporation of samples. Nebulizers. The MVX-7100 system is compatible with most nebulizer types for ICP-MS sample introduction. Teledyne CETAC Technologies can provide the following nebulizers that can be used to address most analytical applications for the MVX-7100 µl Workstation: 13

14 Chapter 1: Introduction to the MVX-7100 µl Workstation o o C-Flow Nebulizer Range. C-Flow nebulizers are available in 50 µl min -1, 100 µl min -1 and 200 µl min -1 flow rates. DS-5 Microflow Concentric Nebulizer. The DS-5 can be used in conjunction with the MVX-7100 system for the introduction of samples in the 3 to 10 µl min -1 flow rate range. NOTE: Contact Teledyne CETAC Technologies if you need additional accessories not listed, need added features to integrate the workstation into your analytical system, or have unique requirements. Research and development of new features and accessories for the workstation, often inspired by customer requests, is a continuing activity at Teledyne CETAC Technologies. Intended Use The MVX-7100 µl Workstation is designed for use as an automation system in analytical laboratories performing chemical analysis of samples, most notably for use in conjunction with inductively coupled plasma mass spectrometry (ICP-MS) instrumentation for trace element measurement. The MVX-7100 system primarily provides sample introduction capability for sub-optimal sample volumes (within the range of 5 µl to 1.5 ml) to ICP-MS instrumentation and it may also be employed as a sample/standard preparation workstation both offline and online, when using the CETAC Workstation operating software, for some aqueous based matrix types. Chemical Compatibility Non-Wetted Components Workstation components are made of corrosion-resistant stainless steel alloys or anodized aluminum. The enclosure and base are made from a high-strength aluminum alloy that is treated with a RoHS-compliant chemfilm and finished with an epoxy powder coating. Long-term exposure to aqueous acids greater than 15% may cause discoloration or bubbling of the epoxy coating. The enclosure is not sealed against liquid or vapor intrusion. Long-term exposure of the internal components to corrosive materials may cause damage. Wetted Components The workstation operates reliably under a wide variety of conditions. Wetted components are made of Polyetherimide (PEI) and Polytetrafluoroethylene (PTFE). When these inert, non-metallic materials are used at temperatures less than 135 C, they can withstand repeated exposure to the following substances: Predominantly aqueous solutions of strong acids (less than 20%) Predominantly aqueous solutions of strong bases (less than 10%) 14

15 Chapter 1: Introduction to the MVX-7100 µl Workstation Common organic solvents such as acetone, alcohols, ethyl acetate, Methylethylketone (MEK), petroleum oils and derived fuels, tetrachloroethylene, toluene, kerosene and xylene. CAUTION WARNING DAMAGE FROM CHEMICAL EXPOSURE Prolonged or repeated exposure to temperatures greater than 135 C and to the following substances can cause failure of the flow path components: Solutions of concentrated acids (greater than 40%). Solutions of concentrated bases (greater than 10% potassium, ammonium, or sodium hydroxides). Partially halogenated hydrocarbons or extremely aggressive organic solvents (chloroform, methylene dichloride, 1,1,2-trichloroethane). CHEMICAL HAZARD Do not use the workstation with substances which could pose a hazard of serious injury to the operator if spilled or injected, such as formic acid. 15

16

17 2 Preparing for Installation Installing the MVX-7100 µl workstation requires preparation. Before you install the workstation, you should evaluate the physical arrangement of the laboratory to choose a suitable location. You should also understand the configuration of the analytical instrumentation to which the workstation will be connected so that the syringe and valve pump modules can be positioned correctly for optimum sample introduction. Once you choose a location, you must carefully unpack the workstation prior to beginning the installation. Choosing a Configuration There are many possible configurations for the workstation. Prior to operating the workstation you will have made decisions regarding the following: Whether the rinse station will use a single or dual rinse configuration. The kind of sample needle that will be installed. The type of sample racks that are required. Whether a temperature-controlled rack (PRM) will be used. Which type of triggering interface will be used between the workstation and the ICP-MS. Before you begin installation, review your plan for the system configuration and make sure you know how you want the sample and solvent solutions to flow through the system. 17

18 Chapter 2: Preparing for Installation Choosing a Location for Installation Choosing a location for the MVX-7100 µl workstation involves evaluating the laboratory environment for the availability of space, liquid waste routing and power. For the system to function optimally, the location you select must meet specific requirements associated with each of these items. The following sections discuss space, water, and power requirements. Proximity to the ICP-MS Instrumentation For the introduction of sample volumes to analytical instrumentation such as ICP-MS, whereby flow rates of greater than 50 µl min -1 will be employed, the length of connecting sample tubing between the Valve Syringe Module (VSM) and a nebulizer is less critical. However, when introducing very small sample volumes via flow rates of less than 50 µl min -1 then it is very important to minimize the length of the connecting sample tubing to ensure that, firstly, the introduction time is not excessive and, secondly, that the volume of carrier solution required to push the sample from a sample loop to the nebulizer does not exceed the capacity of the syringe in place on the Syringe Pump Module (SPM). To ensure easy positioning of the workstation and that the connecting sample tubing is not too long, the accessory modules (SPM and VPM) can be mounted on either side of the workstation and also both on the same side. You must decide on a workstation configuration that works best for your laboratory and applications. Two typical MVX-7100 µl workstation configurations are shown in FIGURE 2-1 and FIGURE 2-2 in relation to different ICP-MS instruments: FIGURE 2-1 The MVX-7100 µl Workstation configured with the VSM on the right hand side, close to the nebulizer positioned on the left side of an Agilent Technologies ICP-MS instrument. 18

19 Chapter 2: Preparing for Installation FIGURE 2-2 The MVX-7100 µl Workstation configured with the VSM on the left hand side, close to the nebulizer positioned on the right side of a Thermo Fisher Scientific ICP-MS instrument. Space Requirements The recommended footprint for the workstation with two accessory modules (VSM and SPM) is shown in the following table. Space requirements for specific workstations may be different, depending on the configuration and accessories. Workstation Dimensions Recommended Space Including Cables/Tubing Height 43.2 cm (17.0") 45.7 cm (18") Width 52.0 cm (20.5") 66.1 cm (26") ** Depth 39.4 cm (15.5") 50.8 cm (20") Weight 24.5 kg (61 lbs) * TABLE 2-1: Physical Characteristics You will need easy access to the back of the workstation to reach the power switches. As a minimum, allow at least 5 cm behind the workstation for cable egress, ventilation, and access to the power switches. Always position the equipment so that it is easy to disconnect the power cord. Work Surface Requirements The workstation must be placed on a sturdy countertop or table. Due to its weight, do not place the workstation on a folding table. During operation, the workstation produces both vertical and horizontal forces. If the work surface is allowed to shake or wobble, the workstation may walk across the surface, liquids may spill, or data quality may be affected. Rinse Solution/Liquid Waste Requirements For most applications, deionized water, a low concentration acid solution (such as HNO 3, 1%) or kerosene would be used as a rinse agent and as a carrier 19

20 Chapter 2: Preparing for Installation solution/rinse agent for sample delivery to a nebulizer under syringe pump action. Place all solution bottles (rinse and carrier/rinse agents) that service the MVX system within two meters of the workstation. Ensure that there is a liquid waste receptacle within two meters of the workstation. The waste receptacle inlet should be at least 30 to 60 centimeters lower than the XYZ autosampler rinse station outlet and set up so that the rinse drain tubing drops directly into the waste receptacle with no coiling and without being submerged below the liquid level of the waste receptacle. CAUTION SUBMERGED DRAIN TUBING MAY CAUSE EQUIPMENT DAMAGE Ensure that the rinse drain tubing is not submerged in the waste liquid. If the tubing is submerged, waste liquid can back up into the tubing. Power Requirements The workstation receives power through the connection to the external desktop power supply. Place the workstation within 1.2 meters of a power outlet. WARNING SHOCK AND FIRE HAZARD Use only the provided power supply. The power supply must be plugged into an outlet which has a protective ground connection. The workstation is intended to operate from DC power supplied through the provided power supply. The power supply is provided power through an AC power source that will not apply more than 240VAC between the supply conductors and ground. A protective ground connection by way of the grounding connector in the power cord is required for safe operation. Ensure that you position the workstation so that the location where the power supply cord plugs into it is easily accessible (is not blocked) and it can be quickly disconnected if needed. In case of hazard, the workstation should be disconnected from the power source. The power supply socket is on the back of the XYZ autosampler below the power switch. Connect the power supply to the autosampler first and then connect a line cord to the power supply. Do not apply power to the power supply until ready to operate the autosampler. 20

21 Chapter 2: Preparing for Installation Unpacking the MVX-7100 µl Workstation WARNING LIFTING HAZARD Two people are required to lift the workstation. Lifting should be done with a person situated on either side of the instrument. Lifting without assistance may cause injury. Inspect external packaging upon receipt for signs of shipping damage. Inspect all items during unpacking and notify the carrier immediately of any concealed damage. Check for any kinked or bent tubing. If the system is shipped or removed from storage during cold weather, allow the packaged equipment to equilibrate to room temperature before opening and exposing to warm, humid air. It is usually sufficient to provide four to eight hours for this purpose. CAUTION EQUIPMENT DAMAGE FROM CONDENSATION If condensation forms on or inside the workstation, allow it to dry thoroughly before connecting it to a power source and operating it. Failure to do so may cause equipment damage. Remove the packing checklist from the shipping container, and check off items against it. Leave accessories in the packing until you are ready to install them. NOTE Keep the factory packaging for use in case the product ever needs to be returned or shipped to another location. 21

22 Chapter 2: Preparing for Installation This page is intentionally blank. 22

23 3 Installing the Workstation If installation services have been purchased, initial installation will be performed by a Teledyne CETAC Technologies-authorized engineer. Installation services include assembly of the system hardware, electrical and liquid flow connection, establishing system communications, sample needle/probe alignment, operating software configuration and operator training. CAUTION Do not use the workstation until the autosampler has been aligned by a CETACauthorized engineer. If the autosampler is not aligned, the sample needle may be damaged. This chapter provides an overview of how to assemble the system and make the power, data, and liquid flow connections. Tools Required for Installation You will need the following tools: A ball-point Allen wrench set (included) A Phillips-head screwdriver A flat-blade screwdriver 7/16 inch, ¼ inch, and ½ inch wrenches Overview of Installation Tasks To install the workstation, you must complete the following tasks. Each of these tasks will be discussed in detail later in this chapter. Installation of the CETAC Workstation Operating Software or the MVX-7100 Agilent Mass Hunter Plugin Attaching the accessory module mounting blocks Mounting the SPM and VPM accessory modules Connecting the power supply to the hardware components Connecting the MVX-7100 µl Workstation to the host PC 23

24 Chapter 3: Installing the Workstation Installation of the SPM and VPM module syringes Installation of the sample needle/probe Alignment of the sample needle/probe Connection of the liquid facing tubing between the main automation unit, VPM and SPM modules Connection of the VPM to an ICP-MS nebulizer Plumbing the rinse station Connection of a trigger cable between the MVX-7100 µl Workstation and an ICP-MS instrument Installation of the sample rack base or the optional PRM module WARNING WARNING LIFTING HAZARD Two people are required to lift the workstation. Lifting should be done with a person situated on either side of the instrument. Lifting without assistance may cause injury. PINCH/PUNCTURE HAZARD Ensure the AC power is off before proceeding with installation. If the power is left on, motors may move unexpectedly and cause injury. Installing the Software Depending on your system configuration, there are several options for software to control the MVX-7100 µl workstation. You will need to use the software during the hardware installation process to test communications and to position the syringe pumps. Install the software on your ICP-MS host PC. Installing the CETAC Workstation Software The CETAC Workstation software is independent of the ICP-MS and can be used to control the CETAC MVX-7100 workstation along with its syringe pumps, the 6-port valve, and optional Peltier rack module. Scripts can coordinate timing with the ICP-MS, allowing you to analyze large numbers of samples unattended. The installer is supplied on the included CD or may be supplied by your Teledyne CETAC representative. 1 Run the installer from the MVX-7100 software CD. For instructions on how to control the workstation, how to write and edit scripts, and how to run scripts, see the help system which is built into the software: 24

25 Chapter 3: Installing the Workstation Installing the Agilent MassHunter Workstation Software If you will be using the Agilent MassHunter Workstation software with the CETAC MVX-7100, you will need to install and configure a plug-in. You do not need to also install the CETAC Workstation software. For information on installing the plug-in, see the CETAC MVX-7100 Plugin For Agilent MassHunter User s Guide on the CD. For information on using the plugin, start MassHunter and click the help button in the plug-in. Installing the Accessory Module Mounting Blocks Most installations include one valve syringe module and one syringe pump module. Some installations include one or more syringe pump modules. The accessory modules can be mounted on either side of the XYZ autosampler. Decide on an arrangement which will allow you to place the valve syringe module as close as possible to the ICP-MS nebulizer. See page 18 for example arrangements. Each accessory module sits atop an accessory mounting block, and the mounting block can be installed on either side of the autosampler. There are two types of mounting blocks. An inside mounting block is designed to attach directly to the XYZ autosampler. An outside mounting block is designed to attach to an inside mounting block. Outside Block FIGURE 3-1 Mounting Blocks. Inside Block You will need a Philips head screwdriver and a set of Allen (hex) wrenches. 1 Turn the power switches on the power supply, XYZ autosampler, and accessory modules (VSM and SPM) OFF. 2 Place an inside mounting block next to the XYZ autosampler on the side where you want it. 3 Check that the black pin is in the position toward the front of the autosampler. If necessary, turn the block over and move the pin to the other hole. 25

26 Chapter 3: Installing the Workstation Back Front FIGURE 3-2 Position of Pin on Mounting Block (Bottom View). 4 Use four hex-head screws to attach the mounting block to the frame of the autosampler. FIGURE 3-3 Attaching the Mounting Block. The installed mounting block should look like this: FIGURE 3-4 Inside Mounting Block After Installation. 5 Install another inside mounting block on the opposite side of the XYZ autosampler. 6 If more than one module is needed on one side of the MVX-7100 µl workstation, then an outside mounting block needs to be fitted to accommodate that module. The outside mounting block can be fitted by positioning the white mounting pins on the side of the block (as shown in FIGURE 3-1) into the spaces in the side of the inside mounting block (as can be seen in FIGURE 3-4) and pushing the outside mounting block down until it is flush with the inside mounting block. 26

27 Chapter 3: Installing the Workstation Mounting the SPM and VPM Accessory Modules 1 Place the syringe pump on the mounting block. The black pins on the block fit into the hole on the underside of the module. 2 Push the module back until the front is flush with the front of the XYZ autosampler. NOTE To remove a pump from its mounting block, slide it forward as far as it will go, then slide it back about 1 mm before lifting it. Connecting the Power Supply It is important to use the appropriate power cord for your country. See Power requirements on page 84. Power Cord Set Requirements on page 87. WARNING FIRE AND SHOCK HAZARD Use only the provided power supply. 1 Turn the power switches on the power supply, XYZ autosampler, and accessory modules OFF. 2 Slide the power supply underneath the back of the XYZ autosampler. Push it back until it touches the sample tray. The power supply rests on the tabletop, and is not mechanically attached to the XYZ autosampler. 3 Connect the XYZ autosampler power cable (supplied with the XYZ autosampler) to one of the 24V connectors on the back of the power supply. 4 Plug the power supply into the 24V connector on the XYZ autosampler. 5 Locate the power/data cables for the accessory modules. FIGURE 3-5 Power/Data Cables. 6 Connect a power/data cable between a 24V connector on the power supply and the POWER connector on the each accessory module (including the Peltier rack module, if any). 27

28 Chapter 3: Installing the Workstation FIGURE 3-6 Communication and Power Connections on Autosampler and Power Supply. 7 Check the plug on the power cord to verify that it is of the correct type for your country. 8 Plug the power cord into a power outlet. 9 Plug the power cord into the power supply. FIGURE 3-7 Connecting the Power Cord to the Power Supply. 28

29 Chapter 3: Installing the Workstation 10 Connect a 9-pin serial cable to the RS232 port on the power supply. FIGURE 3-8 Connecting the Serial Cable to the Power Supply. 11 Connect the other end of the serial cable to the COM 2 port on the XYZ autosampler. FIGURE 3-9 Serial Cable Connected to the back of the Autosampler. Connecting the MVX-7100 µl Workstation to the Host PC Software on the host PC controls both the ICP-MS instrument and the MVX µl workstation. You cannot operate the workstation until you establish a communications interface between the workstation and the host PC. It is through this interface that the host computer directs the operation of the workstation. The workstation supports the following communications protocols: The USB interface is the standard configuration. A virtual COM port is created so that the connection looks like a standard RS-232 serial port to the host PC software. The serial (RS-232) interface is simple and reliable, but the host computer may lack a free serial port. 29

30 Chapter 3: Installing the Workstation Use either a serial cable or a USB cable (not both). Connecting a USB Cable You will need an A-B USB cable. FIGURE 3-10 USB Cable. 1 Power on both the computer and the XYZ autosampler. 2 Plug one end of the cable into the host computer's USB port. 3 Plug the other end into the XYZ autosampler s USB port. The computer screen should display a "New Hardware Found" window. A USB driver must be installed to make the USB port emulate an RS-232 COM port, and the installation must be repeated for each USB connection. FIGURE 3-11 USB Cable Connected to the Autosampler. 4 Allow the Windows Found New Hardware Wizard to use Windows Update to search for a driver for the USB port. 30

31 Chapter 3: Installing the Workstation In most cases, the driver will be found online and installed automatically. This process may take several minutes. If the driver is not installed automatically, insert the CD-ROM and allow the wizard to search the CD-ROM and install the driver (the exact procedure depends on the version of the Windows operating system). For Microsoft Windows 7, you may need to navigate to the FTDI Driver folder on the CD- ROM to find the driver file named ftdiport.inf. The hardware will be identified as an FT 232R USB UART and then as a USB Serial Converter. FIGURE 3-12 Windows Found New Hardware Wizard (Windows 7). 5 When driver installation is complete, make a note of which COM port number was assigned. The COM port number may be displayed in a bubble in the lower right corner of the screen. 6 Confirm that the COM port selected for the USB matches the port selected in the host computer's instrument control software. Connecting a Serial Cable Use either a serial cable or a USB cable (not both). You will need a serial cable equipped with two male DB-9 connectors. 1 Plug one end of the cable into the host computer s serial (COM) port. If the computer has more than one serial port, make sure to use the same port which is selected in the instrument control software. 2 Finger-tighten both screws of the connector. 3 Connect the other end of the cable to the autosampler s COM1 port. 4 Finger-tighten both screws of the connector. 31

32 Chapter 3: Installing the Workstation NOTES If a host computer serial port with a DB-9F, a DB-25M, or a DB-25F connector (9 pin D-subminiature receptacle or 25 pin D-subminiature plug or receptacle) must be used, use an adapter to convert the serial port to a DB-9M. Do not use a null modem adapter. Ensure you are connecting the serial cable to the COM1 port. Connecting the adapter to the COM2 port on the XYZ autosampler will cause a malfunction. When interconnecting any computing devices, keep the communications cables away from sources of electromagnetic or radio frequency (RF) interference, such as electric motors, transformers, fluorescent light ballasts, or RF energy sources. Limit cable runs for RS-232C to less than 16 meters. If these conditions cannot be satisfied, use low-impedance, fully shielded cables to provide satisfactory operation. The cables are available from many sources, but you will need to specify the correct mating connectors and straightthrough (DTE-DCE) wiring. Installing the VSM and SPM Syringes The supplied syringes need to be installed on the VSM and SPM modules. The procedure is the same for both modules. Caution The syringe should be tightened with just your fingers; using pliers or other tools could damage the syringe. 1 Open the MVX-7100 µl workstation software and initialize communication. 2 Use the manual controls to move the barrel of the syringe to 1/2. If you are using the CETAC Workstation software, on the Manual Controls tab, select the address of the syringe pump. The half-height syringe on the valve syringe module is always at address 16; the other syringes are numbered 1 through 15.Click Barrel to 1/2. If you are using a plug-in, 32

33 Chapter 3: Installing the Workstation 3 Screw the syringe onto the valve. Tighten the syringe into place with your fingers. Do not use any kind of wrench or other tool to do this. FIGURE 3-13 Attaching the Syringe to the Valve. 4 Attach the plunger of the syringe to the syringe locking nut on the module mounting fitting. Ensure that the plunger is properly engaged into the locking nut. Tighten the nut using your fingers. Do not use any kind of wrench or other tool for this purpose. FIGURE 3-14 Attaching the Syringe Plunger to the Locking Nut. 33

34 Chapter 3: Installing the Workstation 5 Once the syringe has been successfully attached to the module, return to the software and initialize that particular pump to push the syringe plunger to its starting position. Installing and Aligning a Sample Needle NOTICE Use only the manufacturer-recommended sample needle, which has a length of 3 inches. Using a short or broken sample needle may result in the needle holder crashing into the top of the rinse station. Shield PEEK Nut on Sample Tubing Top Bulkhead Nut Bulkhead Bottom Bulkhead Nut Needle Holder PEEK Nut on Needle Needle Guide Plate FIGURE 3-15 Sample Needle Assembly. 34

35 Chapter 3: Installing the Workstation Ferrule PEEK Nut Quartz needle FIGURE 3-16 Components of the Sample Needle. Tools Required You will need the following tools and materials: Phillips head screw driver 1/4 inch wrench 7/16 inch wrench 1/2 inch wrench New sample needle with a PEEK nut and compression ferrule Installing the Sample Needle 1 Make sure the Z-drive is in the raised position and is at the home position height. 2 Shut down and unplug the MVX-7100 µl workstation. 3 Move the Z-drive to the end of the arm. 35

36 Chapter 3: Installing the Workstation 4 Remove the clear shield. The shield is held by three screws. FIGURE 3-17 Removing the Shield. 5 Remove the guide plate by removing the two screws. FIGURE 3-18 Removing the Guide Plate. 6 Place the PEEK nut and the compression ferrule on the sample needle as shown in FIGURE Push the sample needle (complete with the PEEK nut and compression ferrule) up into the sample needle holder (as shown in FIGURE 3-15). 8 Tighten the PEEK nut on the sample needle with your fingers to temporarily hold the needle in place in the needle assembly. 9 Hold the needle holder in place with the 7/16 wrench and use the 1/4 wrench to tighten the PEEK nut on the sample needle by ¼ turn (as shown in FIGURE 3-19). DO NOT OVERTIGHTEN you can always return and tighten the nut further if that is required. 36

37 Chapter 3: Installing the Workstation 10 Carefully replace the guide plate. Align the needle in the guide plate and gently push the guide plate straight up. Install the two screws to hold the guide plate in place. FIGURE 3-19 Loosening the Needle from the Needle Holder. Adjusting the Sample Needle Height 1 When the sample needle has been fitted into place, ensure that it is positioned at the correct height. The top of the sample needle should be approximately flush with the top nut on the bulkhead (as shown infigure 3-20) whilst the bottom of the needle should not exceed beyond 2 mm below the guide plate (as shown in FIGURE 3-21) and preferably not more than 1 mm. If you are using sample needles that have a greater than standard length then you will need to adjust the thumbscrews on the needle assembly to lower the height of the guide plate and the range of motion of the sample needle. If you need to adjust the height of the guide plate you should also check the height of the standards, calibrate the Z-height using the software and verify that there is adequate clearance for the needle above all components in the sample area. FIGURE 3-20 Top of the Sample Needle Approximately Flush with the Top Nut of the Bulkhead. 37

38 Chapter 3: Installing the Workstation FIGURE 3-21 Plate. Sample Needle Protruding Approximately 1 mm Below the Guide Testing the New Needle 1 Make sure the Z drive clear of any obstructions. 2 Power up the MVX-7100 µl workstation (the arm and needle assembly will return to the HOME position). 3 Manually operate the short syringe on the VSM module to push some rinse solution through the sample needle. Visually check the sample needle connections (particularly between the PEEK nut and the needle holder and at the bottom of the PEEK nut) for leaks when you are pushing rinse solution through it. Tighten the PEEK nut as previously described. 4 Reinstall the clear shield with the three screws. Aligning the Sample Needle The autosampler must be aligned before it is used for the first time. Initial alignment is performed on-site by a CETAC-authorized engineer. Following the installation of a sample needle or a major event such as the uninstallation of the MVX-7100 µl Workstation and movement from one laboratory bench to another or one laboratory to another it is strongly recommended that the sample needle position be aligned so to ensure that the needle moves to the desired position and does not engage with the side of the rinse station or a sample tube and be damaged. An alignment utility is provided on the CD. The alignment procedure is continually being improved; contact CETAC to make sure you get the latest alignment software and procedure. Installing Fittings on the Tubing Some of the plumbing tubing for the MVX-7100 µl workstation, such sample loops, comes with fittings (nuts and ferrules) already installed. For other tubing, such as the line between the SPM module and an ICP-MS nebulizer, 38

39 Chapter 3: Installing the Workstation fittings will need to be installed before they can be connected to corresponding ports such as those on the six port switching valve. There are three different types of tubing fitting that are associated with the MVX-7100 µl workstation and each are attached differently. If you need to fit or replace any of these fittings, the description of their installation is as follows. All fittings should be tightened to finger tight only and not with wrenches or similar tools. It is important to trim the tubing so that it does not extend very far past the ferrule; excess tubing can block liquid flow or even interfere with the movement of the valve. 1 Pass the tubing through the nut. 2 Place the ferrule on the end of the tubing. 3 Very gently screw the nut with the ferrule and tubing in place part way into the corresponding port. 4 Push the tubing all of the through the nut to the furthest most point of the corresponding port. 5 Tighten the nut with your fingers. 6 Unscrew the nut and remove the tubing with nut and ferrule attached. 7 Trim off any tubing which extends beyond the the end of the ferrule. Nut Ferrule Cut Tubing Here FIGURE 3-22 Trimming Off the Excess Tubing. 8 Screw the nut, complete with ferrule and tubing, back into the corresponding port. Connecting Tubing to the VSM and SPM The plumbing connections depend on the application of the MVX-7100 µl workstation and on its particular setup/configuration. This section describes the most common setup with one VSM module and one SPM module. In order to start connecting the tubing to the VSM and SPM, please familiarize yourself with the components of the six-port switching valve and the T-shaped switching valve (or Y-shaped switching valve) that is connected to the syringe of the VSM as shown in FIGURE 3-23 and the T-shaped switching valve (or Y- 39

40 Chapter 3: Installing the Workstation shaped switching valve) that is connected to the syringe of the SPM as shown in FIGURE Ports 3 & 6: Loop Port 2: Syringe Port 4: Rinse Port 1: Needle Port 5: ICP-MS Input: Rinse Solution or Solvent Output: 6-Port Valve FIGURE 3-23 Six-Port Switching Valve on a VSM Module. Output: 6-Port Valve Input: Rinse Solution or Solvent FIGURE 3-24 VSM and SPM Modules. 40

41 Chapter 3: Installing the Workstation 1 Connect the sample loop between Port 3 and Port 6 of the six port switching valve. 2 Connect the sample line from the sample needle top bulkhead nut to Port 1 of the six-port switching valve. 3 Connect the buffer volume line (a 1.25 ml capacity loop line) between the output (left hand side as the user looks at the valve) of the half height syringe switching valve on the VSM to Port 2 of the six port switching valve. 4 Connect the carrier solution line between the output (left hand side as the user looks at the valve) of the full height syringe switching valve on the SPM to Port 4 on the six port switching valve. 5 Connect a line between Port 5 of the six port switching valve to the nebulizer of the ICP-MS, as described on page Connect a carrier solution source line to the input (right hand side as the user looks at the valve) of the full height syringe switching valve of the SPM and place the other end of the line into a source bottle for the carrier solution. The tubing for this should be long enough to reach the source bottle and touch the bottom of the bottle. Ensure that the tubing is routed such that it does not become entangled with any moving parts of the workstation. 7 Connect a rinse line to the input (right hand side as the user looks at the valve) of the half height syringe switching valve of the VSM and place the other end of the line into a source bottle for the rinse solution. The tubing for this line should be long enough to reach the source bottle and to touch the bottom of the bottle. Ensure that the tubing is routed such that it does not become entangled with any moving parts of the workstation. Output: 6-Port Valve Input: Rinse Solution or Solvent FIGURE 3-25 Syringe Pump Module Valve Connections. 41

42 Chapter 3: Installing the Workstation Connecting the VPM to an ICP-MS Nebulizer The SPM module pumps the sample into the ICP-MS nebulizer, via the six-port switching valve. Therefore, the sample tubing that connects the workstation to an ICP-MS should be routed directly from Port 5 of the six-port switching valve to the nebulizer. Do not route the sample line through the ICP-MS peristaltic pump; this is no longer required for sample introduction and is only used for the draining of the ICP-MS spray chamber. 1 Position the MVX-7100 µl workstation as close to the ICP-MS as possible. 2 Cut a length of x sample tubing to the shortest length possible which will allow connection of Port 5 to the nebulizer without any kinking. 3 Connect the tubing to Port 5 of the 6-port valve and to the nebulizer. The nebulizer that is being used will dictate how this line can be connected at the nebulizer end. If unsure, please contact Teledyne CETAC Technologies for further information. If a longer length of tubing is required, cut a new piece of tubing. Avoid splicing the tubing; splices create small voids which can increase carryover. Connecting Tubing to the Rinse Station The rinse station is located at the left end of the standards rack at the back of the sample rack base. Typically, deionized water, an acid rinse such as a 2% HNO 3, or a solution from the kerosene family, is used as the rinse solution and is pumped into the rinse station by the peristaltic pump on the back of the autosampler. You can use one or both channels of rinse station as required by your application and the same or different solutions can be pumped to the two channels at the same time by action of the peristaltic pump. If your application requires only one channel, use channel 1 (the channel on the left hand side as you look at the rinse station). See FIGURE 3-26 for a visual representation of the flows and channels of the rinse station. 42

43 Chapter 3: Installing the Workstation FIGURE 3-26 Rinse Station Showing: i) Channels 1 and 2 of the Dual Rinse Station; Ii) the Input Ports for Channels 1 and 2; Iii) the Drain Line Out of the Rinse Station. The input flows for the rinse station can be connected via either threaded nut and ferrule fittings (used for narrow ID tubing) or via barbed fittings (for wider ID tubing). Direction of Flow Since the inlets of the rinse station are at the bottom of the rinse station, the rinse solution flows from the bottom to the top. Up-flow rinsing is the most effective method for decontaminating the sample needle between samples. The used rinse solution flows into a drain reservoir within the rinse station after exiting the top of a channel. The solution then drains to a waste container. Rinse Solution Recycling For some applications it may be possible to recycle the rinse solution by draining the rinse solution into the same container from which it was drawn. Be sure to change the rinse solution on a regular basis, as required by the application. If the application requires that the rinse solution be completely uncontaminated, however, use separate containers for the clean and waste rinse solution. Gravity Drain Arrangement Make sure that the end of the waste line remains above the level of the liquid in the waste container. The rinse solution flows from the station channel to the drain (rinse out) reservoir within the rinse station. The waste rinse can then be pumped from the drain reservoir or allowed to drain under gravity. 43

44 Chapter 3: Installing the Workstation The advantages of gravity drain arrangements are: Only one rinse pump channel is required Tubing connections are simpler Pumped Drain Arrangement If the application requires a very high flow rate of the rinse solution, waste rinse solution may need to be pumped from the rinse station to prevent overflow. Pumping may also be necessary if the waste receptacle is far from the rinse station, or if there is a chance that the end of the waste tube may become submerged in the waste liquid. Tubing Considerations Verify that the supplied tubing material is compatible with the rinse solution you are using. There are many different types of tubing that can be used for the rinse station. Clear Tygon tubing is provided. Viton, Tygon Fuel and Lube (yellow), and PharMed (opaque) may also be used for pump tubing depending on the application and user preference. Clear Superthane tubing can used everywhere other than the pump tubing. Contact Teledyne CETAC Technologies if you need different tubing material. For barb type fittings, carefully press the tubing straight on to the fittings to avoid breaking them. The tubing should completely cover the barb of the fitting. Connecting the Tubing to the Peristaltic Pump On the rear of the XYZ autosampler there is a peristaltic pump that drives liquid reagents into and out of the rinse station. FIGURE 3-27 Peristaltic Pump on the Rear of the XYZ Autosampler. Tubing should be connected between this pump and the rinse station as follows: 1 On the back of the XYZ autosampler, connect the rinse tubing to one or both channels of the output of the peristaltic pump (as shown infigure 3-27). Connect the other end of each tube that is used to the input port(s) of the rinse station (as shown in FIGURE 3-26). 44

45 Chapter 3: Installing the Workstation 2 Connect rinse tubing to the input(s) of the peristaltic pump. Place the other end of this tubing into the rinse solution source bottle. Ensure that the tubing can reach the bottom of the rinse source bottle. 3 Connect up to 1.8 meters of the larger 3 / 16 inch (4.8 mm) ID tubing between the outlet drain fitting of the rinse station and the waste container. Ensure that the tubing in the waste bottle remains above the surface of the liquid. If the waste tubing is immersed in the waste bottle then solution may back up and overflow the rinse station. Installing the Sample Rack Base or the Optional PRM Module Sample racks or well plates are mounted on a sample rack base during operation of the MVX-7100 µl Workstation. There are two options for the sample rack base: Sample Rack Base The standard sample rack base that is offered for mounting racks and well plates is a simple frame that can accept two racks or plates or a combination of the two. FIGURE 3-28 Standard Sample Rack Base. Peltier Rack Module The MVX-7100 µl workstation can also be supplied with a peltier rack module (PRM) for mounting sample racks and well plates for analysis. The PRM provides temperature control between 4 C and 40 C. Temperature control at the lower end of this range is particularly important for stabilizing low sample volumes and samples that are more volatile and to limit evaporation. Temperature control at the higher end of this range can be useful for some sample preparation applications. 45

46 Chapter 3: Installing the Workstation FIGURE 3-29 Peltier Rack Module. Both the standard sample rack base and the PRM module are fitted to the rack adapter plate on the base area in front of the standards position rack and the rinse station. Both the standard rack base and the PRM are positioned with four feet into four corresponding slots in the rack adapter plate. When a standard rack base or PRM is positioned on the rack adapter plate, sample racks or well plates can then be positioned on top. Two sample racks or well plates (or one of each) can be positioned and used for analysis at any one time. 46

47 4 Verifying Installation Once installation of the MVX-7100 µl workstation is complete, it is important to verify that you have installed it correctly. Attempting to use it before ensuring that it is installed correctly may result in damage to the system. Verification consists of the following actions which are described in this chapter: Opening the CETAC Workstation operating software and ensuring that the communications interface between the workstation hardware and the host computer is working correctly. Checking the alignment of the sample needle with respect to the rinse station. Checking the alignment of the sample needle with respect to the sample tubes or well plate wells. Verifying Communication with the MVX-7100 µl Workstation 1 Check that the workstation is connected to the host computer (either by USB or RS232 cable connection, see page 29 for details) and apply power to the XYZ autosampler and associated modules. 2 Double-click the CETAC Workstation shortcut (which should have been established on the host computer desktop during installation). FIGURE 4-1 Icon for the CETAC Workstation operating Software. 3 When the CETAC Workstation operating software has opened, select the COM port from the Home tab. If the communications interface is working correctly 47

48 Chapter 4: Verifying Installation between the workstation and the host computer then the software will have automatically detected the COM port that is required. 4 Click Initialize Communication. The operating software will take 5 to 10 seconds to initialize. 5 Click the Manual Controls tab. 6 Examine the choices under Pump Address and establish that the addresses for the VSM and SPM(s) that are associated with the workstation are present. Address 16 corresponds to the VSM and addresses 1 to 15 correspond to the SPMs that are in place. The standard configuration of one VSM and one SPM will be represented as addresses 1 and Click the Autosampler tab. 8 Click the home button to ensure that the XYZ autosampler is in the home position. FIGURE 4-2 Home Button. 9 From the home position, move the sample needle down and up by a couple of mm in the Z-direction (via the Z-direction up and down arrows as shown in FIGURE 4-3 Z Movement Controls.; the magnitude of movement can be established in the drop down menu to the left of the arrow buttons) to ensure that there is movement control of the hardware by the operating software. FIGURE 4-3 Z Movement Controls. How to Tell If There Is a Communication Problem If the computer is not communicating with the workstation, then the CETAC Workstation operating software will start in offline mode and display the Platform Configurator window. If a syringe or other accessory module is not communicating with the workstation, the address will not be displayed on the Manual Controls tab. If there is a communication problem, make sure that all of the devices are turned on and that communication cables are attached securely. The accessory modules communicate through their power cables. 48

49 Chapter 4: Verifying Installation Checking the Sample Needle/Sample Tube or Well Alignment To ensure that the sample needle is aligned to the sample positions whether that be a sample vial, tube or well plate well follow these steps: 1 Click the Autosampler tab. 2 Click the home button.this ensures that the sample needle is positioned over the workstation rinse station and that it is in its highest position. FIGURE 4-4 Home Button. 3 Select Tube # 1 in the field immediately to the right of the Home button. FIGURE 4-5 Select Tube Field. 4 Click the arrow button immediately to the right of the Select Tube# field. This moves the sample needle to position number 1 in the X and Y directions but will not lower the needle in the Z-direction. 5 Slowly move the sample needle (Z axis) and confirm that it is centered over the rinse station. FIGURE 4-6 Z Movement Controls. 49

50 Chapter 4: Verifying Installation 6 After ensuring that the needle is centered, raise the needle and return to the home position. 7 If desired, check the alignment at another sample position. NOTE: If the sample needle alignment at the sample position is not correct then the needle alignment will need to be repeated. If you continue to experience difficulty with this alignment please contact Teledyne CETAC Technologies Customer Service and Support or an authorized representative. 50

51 5 Using the Workstation Before using the workstation, ensure that your lab environment provides operating conditions that will prolong the life of the workstation. Once the proper operating conditions are met, you can arrange the sample vial racks and start the workstation sequence run. When you finish using the workstation, you may need to flush the rinse station and flow path before shutting the workstation down. Be sure to review the safety precautions beginning on page 87 before you begin to use the workstation. WARNING PUNCTURE HAZARD Never attempt to load, unload or reposition the sample vial racks or sample vials while the workstation is operating. The sample needle may move unexpectedly and cause an injury. Establishing Optimal Operating Conditions The workstation operates reliably even under less than ideal conditions. It is not, however, indestructible. Malfunction or damage can occur if specific operating conditions are not met. Meeting these conditions requires that you create the proper lab environment, replace workstation components that wear out under normal use, and purchase the appropriate supplies for use with the workstation. The following sections explain how to meet these conditions. 51

52 Chapter 5: Using the Workstation NOTE Damage or malfunction that results from unsatisfactory operating conditions may constitute misuse and abuse and be excluded from warranty coverage. Creating the Lab Environment To create satisfactory operating conditions in your lab environment, follow these guidelines: Operate the workstation in a conventional lab environment where the temperature is F (10 35 C), the humidity is 20 70% non-condensing, and the unit is not exposed to excessive flammable or corrosive materials. Avoid rough handling of the workstation. If possible, do not expose the workstation to vibration or shock. Protect the workstation from long-term exposure to condensation, corrosive materials, solvent vapor, continual standing liquids, or large spills into the workstation cabinet or arm. Exposures of this type can damage the drive mechanisms as well as the electronics. Observe the same general electrostatic discharge precautions as with any other integrated circuit electronic devices. Low humidity environments, especially when combined with static-generating materials, require maximum care. CAUTION Discharge static buildup and ground to the autosampler base or cabinet before performing any maintenance. Do not touch or short-circuit the communication ports. Avoid using the workstation if strong electromagnetic interference, radio frequency interference, or radioactivity is present. Interference fields can cause erratic operation of the workstation. The workstation will not function properly if the level of radioactivity is above background. Purchasing Supplies Because the life-span of the sample and reagent vials varies, you should maintain an adequate supply of spare vials. When you need to purchase additional supplies, it is extremely important that you choose the appropriate sizes and materials. When you purchase sample and/or reagent vials, make sure they meet the following requirements: The diameter of the sample or reagent vial matches the rack size you are using. The height does not interfere with motion of the sample needle. The material is compatible with the samples you are analyzing or the reference standards you are using. This requirement also applies to the peristaltic pump tubing. 52

53 Chapter 5: Using the Workstation CAUTION Use of mismatched sample vials and sample vial racks may result in malfunctions or sample spills. Be sure your vials meet the given requirements. To order additional supplies, refer to the CETAC Accessories and Supplies Catalog for the workstation, available on the CD or on the CETAC Web site, Arranging the Sample Vial Racks Make sure that the arrangement of vials and racks matches the arrangement defined in the software which is controlling the workstation. CAUTION Incorrectly defining the arrangement of vials and racks can result in damage to the needle, sample spills, and invalid analysis results. Using Septum Vials Special care is required when using septum vials due to the use of the glass needle. After the needle pierces a septum, the septum deforms slightly. If the needle pierces the septum at another location on the septum, horizontal forces are applied to the glass needle which may cause it to break. Therefore, pierced septa should be replaced whenever vials are moved in any way. If the needle reenters the vial at precisely the same location, horizontal forces are minimal. Multiple samples may be drawn from a vial without replacing the septum if the vial has not been touched. CAUTION Use new septa to prevent damage to the needle. Starting the Workstation Once you arrange the sample vial racks and ensure that the arrangement is correctly defined in the software, you can start the workstation and let it run until the sampling sequence is finished. WARNING PINCH HAZARD Keep fingers, hair, and loose clothing away from the moving parts of the workstation. 1 Ensure that the rinse station is properly connected. 2 Manually move the Z drive to the left and toward the end of the arm. 53

54 Chapter 5: Using the Workstation The sample needle will briefly dip down when the power is turned on, then move to the home position. Moving the Z drive to a safe position ensures that the sample needle will not collide with anything. 3 Turn the power switch on the power supply ON. 4 Turn the power switches on the autosampler and accessory modules ON. 5 Purge air from the rinse system by placing the rinse solution uptake tubing in the rinse solution source and running the rinse solution through the rinse station. Ensure there are no air bubbles visible in the rinse uptake tubing before you run samples with the workstation. NOTE FOR AQUEOUS APPLICATIONS If you are flushing the rinse system during initial startup, first use a 2% nitric acid solution as the rinse agent. Flush the rinse system a second time using deionized water as the rinse agent. 6 Access the host computer s software and activate the workstation program. The workstation runs until it reaches the end of the sampling sequence. Shutting Down the Workstation To shut down the workstation, complete the following steps: 1 Drain the rinse system by removing the rinse solution uptake tubing from the rinse solution source. Let the peristaltic pump run until all solution drains from the tube attached to the rinse station outlet. You may wish to create a cleanup method which will flush the syringes and tubing. If you use a rinse solution other than deionized water, flush the rinse system with deionized water before shutting down the workstation. For more information, see Flushing the Rinse Station and Flow Path on page Turn the power switches on the autosampler and accessory modules OFF. 3 Turn the power switch on the power supply OFF. 4 If you will be performing maintenance, unplug the power cord either at the power supply or at the wall outlet. Flushing the Rinse Station and Flow Path Generally, you can operate the workstation without flushing the rinse system. Under normal circumstances, you can simply drain the rinse system prior to shutting down the workstation. However, you need to flush the rinse station and flow path under two circumstances: 54

55 Chapter 5: Using the Workstation During initial startup of the workstation after installation. After the use of strong bases, acids, or organic solvents as rinse agents. Flushing the rinse system during initial startup of the workstation removes any contaminants that could cause interference during sample analysis. Flushing the rinse system after using strong rinse agents prevents degradation and failure of the flow path components. To flush the rinse station and flow path, complete the following steps: 1 Insert the rinse uptake tubing into a deionized water source. NOTE If you are flushing the rinse system during initial startup, first use a 2% nitric acid solution as the rinse agent, followed by deionized water. 2 Run the rinse solution through the rinse station and flow path for 5 to 10 minutes. Once you flush the rinse system, you can proceed with the sampling sequence or drain the rinse system as part of the shutdown procedure. Calibrating the Z Depth The Z depth determines how far the sample needle descends. There are two Z depths which can be set independently: one for the standards vials and rinse station, and one for the sample vials or wells. When to calibrate the Z depth CAUTION Set the Z depth before using the autosampler. Operating with an incorrect Z depth can damage the sample needle. The CETAC Workstation software will ask you to set the Z depth for each new script you create. You should also set the Z depth when: A new brand or size of vial is being used. Vial racks have been replaced or rearranged. There is any possibility that the height of the standards rack has changed. The sample needle has been adjusted or replaced. How to calibrate the Z depth Place a reagent bottle in the first standard position, and place a sample vial or well plate in the first sample position (rack 1, position 1). 1 Click the Calibrate Z Depth tab. 2 Click Go To Stnd Location. 3 Set the movement step to 5.0 mm. 55

56 Chapter 5: Using the Workstation 4 Click the down arrow until the sample needle is about 1 cm above the bottom of the vial. 5 Set the movement step to 1.0 mm. 6 Use the up and down arrows to adjust the height of the sample needle. 7 When the desired height has been reached, click the mark button. 8 Click Go To Rack Location and repeat the process for the first sample location. Writing and Running Scripts with the CETAC Workstation Software The CETAC Workstation software is used to control the CETAC MVX-7100 workstation along with its syringe pumps, the 6-port valve, and optional Peltier rack module. Scripts can coordinate timing with the ICP-MS, allowing you to analyze large numbers of samples unattended. If you run the software on a PC which is not connected to an MVX-7100 workstation, you can: Create scripts Edit scripts If you run the software on a PC which is connected to an MVX-7100 workstation, you can: Manually control workstation components including the autosampler, valves, syringe pumps, and Peltier temperature controller Calibrate the Z depth of the sample needle Create scripts Edit scripts Run scripts To use the CETAC Workstation software: 1 Check that the workstation is connected to the PC and that it is turned on. 2 Double-click the CETAC Workstation icon. The software will automatically detect the COM port where the workstation is connected. 3 On the Home tab of the CETAC Workstation software, click Initialize Communication. 56

57 Chapter 5: Using the Workstation CAUTION Always check that the hardware configuration set in the software matches the actual configuration of the system. The type of rack or well plate which is selected is very important; if the wrong type of rack is selected, the sample needle may crash into the rack and break. 4 For instructions on how to control the workstation, how to write and edit scripts, and how to run scripts, see the help system which is built into the software: 57

58 Chapter 5: Using the Workstation This page is intentionally blank. 58

59 6 Maintaining the Workstation Routine maintenance of the workstation consists of daily and weekly cleaning of specific workstation components. Routine maintenance also includes checking for leaks or other damage. Additional periodic maintenance tasks may be required, including replacement of workstation components such as the peristaltic pump tubing, sample needle, or rinse station tubing,. CAUTION Discharge static buildup and ground to the workstation base or cabinet before performing any maintenance. Do not touch or short-circuit the communication ports. Cleaning the Workstation Cleaning the workstation is the primary maintenance task you perform. Failure to do so regularly causes increased wear and reduces the workstation s life. You must clean the workstation both daily and weekly to prevent damage and extend its life. It is especially important to clean up spills and remove contaminants, such as abrasives, from the workstation s moving parts. It may also be necessary to chemically neutralize spills. The following sections explain daily and weekly cleaning procedures. Daily External Cleaning Use of the workstation often results in spills on workstation components such as the sample tray. Good maintenance requires that you clean the workstation daily. To do so, complete the following steps: 1 Shut down and unplug the workstation. For information about shutting down the workstation, see page Wipe the sample tray, workstation cabinet, and autosampler arm using a towel dampened with a lab-grade cleaning agent. 59

60 Chapter 6: Maintaining the Workstation CAUTION Do not allow the cleaning agent to come into contact with the lead screws. Also, never lubricate either of the two lead screws. 3 Repeat step two, using a towel dampened with clear water. This process removes any remaining contaminants. 4 Dry the sample tray, workstation cabinet, and autosampler arm using a dry towel. The workstation must be thoroughly dry before you turn the power on. Weekly Cleaning Although cleaning it daily removes spills and contaminants from most of the workstation components, it is necessary to clean the workstation more thoroughly once a week. To do so, complete the following steps: 1 Shut down and unplug the workstation. 2 Remove the sample tray. For information about removing the sample tray, see Replacing the Sample Tray later in this chapter. 3 Wipe loose particles off the Y-Axis lead screw with a dry, lint-free cloth. The Y-Axis lead screw is a large metal screw located inside the autosampler arm. CAUTION Never lubricate the lead screws. The lead screw nuts are compounded with a dry film lubricant. Oiling the lead screws will cause gumming, galling, and binding of the sample needle assembly. 4 Wipe the workstation exterior and base until they are clean, using a towel dampened with a lab-grade cleaning agent, followed by a towel dampened with clear water. Pay special attention to the slider block and guide rails along the tube of the autosampler arm. 5 Wash the sample tray in a warm detergent solution. Make sure you remove all spills and stains. 6 Rinse the sample tray with water and then dry it. Ensure that the sample tray is thoroughly dry. 7 Replace the sample tray on the autosampler base. For information about replacing the tray, see Replacing the Sample Tray later in this chapter. 60

61 Chapter 6: Maintaining the Workstation Checking for Leaks It is important that you check all of the components for damage before you operate the workstation. To do so, complete the following steps: 1 Shut down and unplug the workstation. 2 Visually inspect the sample needle, syringes, fittings, and tubing for leaks or signs of deterioration. If you detect a leak or other damage to a workstation component, you must replace it. For more information, see the appropriate section in this chapter. Replacing Peristaltic Pump Tubing Routine maintenance of the autosampler includes replacement of the peristaltic pump tubing. This tubing will probably be the most frequently replaced item on the autosampler. If you use strong bases, acids, or solvents as rinse agents, the tubing may break down rapidly. The tubing is installed as a single cartridge. The plates at the ends of the cartridge are color coded according to the tubing size and material. FIGURE 6-1 Peristaltic Pump Tubing Cartridge. To replace the peristaltic pump tubing, complete the following steps: WARNING PINCH HAZARD Turn off power before servicing the peristaltic pump or pump tubing. 1 Shut down and unplug the autosampler. 2 Release the pressure shoe by lifting the handle. The shoe is a flexible band which is held in place by spring-loaded handles. 61

62 Chapter 6: Maintaining the Workstation FIGURE 6-2 Releasing the Peristaltic Pump Shoe. 3 Lift the end of the tubing cartridge straight up. FIGURE 6-3 Removing the Peristaltic Pump Tubing Cartridge. 4 Replace the tubing by sliding the plates on the end of the tubing cartridge into the slots in the pump. 5 Reinstall the pressure shoe. 62

63 Chapter 6: Maintaining the Workstation Replacing the Sample Needle You must replace the sample needle if it breaks or shows other signs of deterioration. NOTICE Don't try to adjust the Z depth to compensate for a broken or shortened sample needle. Use only the manufacturer-recommended sample needle, which has a length of 3 inches. Using a short or broken sample needle may result in the needle holder crashing into the top of the rinse station. Shield PEEK Nut on Sample Tubing Top Bulkhead Nut Bulkhead Bottom Bulkhead Nut Needle Holder PEEK Nut on Needle Needle Guide Plate FIGURE 6-4 Sample Needle Installed in Z Drive. Tools Required You will need the following tools and supplies: Phillips screw driver 1/4 inch wrench 7/16 inch wrench 1/2 inch wrench New sample needle with PEEK nut and compression ferrule Removing the Old Needle 1 Make sure the needle is in the raised position. 63

64 Chapter 6: Maintaining the Workstation 2 Shut down and unplug the workstation. 3 Pull the Z drive left and then out to the end of the arm. 4 Remove the clear shield (3 screws). FIGURE 6-5 Removing the Shield. 5 Remove the guide plate (2 screws). If the sample needle is not already broken, pull the guide straight down, being careful not to break the sample needle. FIGURE 6-6 Removing the Guide Plate. 6 Use the 7/16 wrench to hold the needle holder, while using the 1/4 wrench to loosen the nut on the sample needle. 64

65 Chapter 6: Maintaining the Workstation FIGURE 6-7 Loosening the Needle from the Needle Holder. 7 Pull the probe straight down to remove it. Installing the New Needle 1 Place the PEEK nut on the needle. 2 Place the compression ferrule on the needle. Ferrule PEEK Nut Quartz needle FIGURE 6-8 Assembling the Sample Needle. 3 Push the needle straight up into the needle holder. 4 Tighten the PEEK nut with your fingers. 5 Use the 7/16 wrench to hold the needle holder still, while using the 1/4 wrench to tighten the nut on the needle 1/4 turn. Do not over-tighten. You can always come back and tighten the nut more, if needed. 65

66 Chapter 6: Maintaining the Workstation 6 Carefully replace the guide plate. Align the needle in the guide plate and gently push the guide plate straight up, then install the screws. Adjusting the Sample Needle Height 1 Use the 1/2 and 7/16 wrenches to loosen the sample needle holder from the bulkhead. 2 The top of the nut on the needle should be about flush with the top nut on the bulkhead. FIGURE 6-9 Proper Sample Needle Height (Top View). Adjust the sample needle so that about 1 mm (not to exceed 2 mm) of the needle tip extends below the guide plate. FIGURE 6-10 Proper Sample Needle Height (Bottom View). If you are using a different length needle, you may need to use the thumbscrews to adjust the height of the guide plate and the range of motion of the sample needle. In that case, you should also check the height of the standards, calibrate the Z height using the CETAC Workstation software, and verify that there is adequate clearance for the needle above all components in the sample area. 3 Turn the needle holder so that the flat edge faces toward the front of the autosampler. 4 Tighten the nut on the needle holder. 66

67 Chapter 6: Maintaining the Workstation Testing the New Needle 1 Make sure the Z drive clear of any obstructions. 2 Start the workstation. 3 Manually operate the syringe to push some rinse solution through the sample needle. 4 Visually check for leaks. If necessary, tighten the nut another 1/4 turn. 5 Reinstall the clear shield. Replacing the Rinse Station Tubing If the rinse station tubing is typically exposed to deionized water as a rinsing agent, you do not need to replace it often. However, if you use other rinsing agents, such as acids or solvents, the tubing is likely to deteriorate more rapidly. To replace the rinse station tubing, complete the following steps: 1 Shut down and unplug the workstation. 2 Remove the sample rack. 3 Move the autosampler away from the home position by gently pushing it. Moving the autosampler arm ensures that the sample needle will not be damaged while you replace the rinse station tubing. 4 Disconnect the rinse solution uptake and drain tubing. Apply only a linear force when removing the tubing to prevent the fittings from breaking. 5 Reconnect the rinse solution uptake and drain tubing. Apply only a linear force when replacing the tubing to prevent the fittings from breaking. 6 Move the autosampler arm back to the home position. 67

68 Chapter 6: Maintaining the Workstation Replacing the Battery The battery in the autosampler is used to store diagnostic data in SRAM memory. The battery does not need to be replaced for routine operation of the workstation. WARNING FIRE AND EXPLOSION HAZARD Replace only with a CR2032 (3.0V, non-rechargeable lithium coin cell) or equivalent battery. Using an incorrect battery may cause fire, explosion, or leakage, resulting in personal injury or equipment damage. Do not open battery, dispose in fire, heat above 100C (212F), expose contents to water, recharge, or put in backwards battery may explode or leak and cause personal injury. 1 Turn off the power switch on the back of the autosampler. 2 Unplug the autosampler from the power supply. 3 Disconnect all of the various serial and other cables that normally attach to its back. 4 Remove the screws that hold the cover to the case. There are two screws on the front, and either two or four screws on the top of the autosampler. FIGURE 6-11 Location of Screws for the Top Cover. 5 Carefully pull the cover from the case. 68

69 Chapter 6: Maintaining the Workstation Battery FIGURE 6-12 Location of the Battery. 6 Note the orientation of the battery, then pull it out. 7 Insert the new battery. 8 Replace the cover. 9 Reconnect the power cord and turn on the power switch. 10 Recycle or dispose of the battery in accordance with local regulations. Replacing the Fuse WARNING FIRE AND SHOCK HAZARD Replace only with a 5A 250V slow-blow 5x20 mm fuse ( T5AL250V). Using an incorrect fuse may cause fire or personal injury. Two fuses are located in the power supply, just above the power cord connector. Fuse Holder 69

70 Chapter 6: Maintaining the Workstation 1 Disconnect the power cord. 2 Inspect all of the equipment which is plugged into the power supply for moisture or other conditions which might pose a hazard and cause the new fuse to blow. 3 Using your fingernails or a small, flat-blade screwdriver, squeeze the ends of the fuse holder. 4 Pull the fuse holder out. 5 Replace the blown fuse with a new one of the same size, type, and rating. FIGURE 6-13 Fuse. 6 Press the fuse holder back in until it clicks into place. 7 Plug the power cord back in. 70

71 7 Troubleshooting the Workstation In the event that the product does not function properly, isolate the problem to determine if it originates in the host computer, the analytical instrument, or the workstation. This chapter explains how to troubleshoot workstation problems. If you cannot solve a problem using the steps given in this chapter, you should contact Teledyne CETAC Technologies Customer Service and Support. Power System Problems A possible cause of system malfunction is a problem in the power system. If the system is not functional, it is possible that it is not receiving power. If this is the case, both of the LED status indicator lights will be off. To troubleshoot this problem, complete the following steps in sequence until the problem is solved: 1 Check that the power switch on the workstation is turned on. 2 Check that the power cord is plugged in firmly between the power input on the workstation and the external power supply and also between the power supply and the wall outlet. If the cable is plugged in, ensure that it is not damaged in any way. 3 Check that the power supply is turned on. 4 If the external power supply is plugged into the autosampler and turned on, cycle the autosampler power switch. The sample needle should move up and the autosampler should initialize. After initialization, the status LED on the front of the autosampler should light up. 71

72 Chapter 7: Troubleshooting the Workstation Communications Interface Problems Operation of the workstation is directed by the host computer. A malfunction can indicate a problem with the cable or with the configuration of the software on the host computer. The following sections explain how to troubleshoot these problems. RS-232 Cable Problems The first step in troubleshooting communications interface problems is to check the RS-232 cable. 1 Check the RS-232 cable to ensure it is plugged in to the COM1 port on the XYZ autosampler. If the cable is plugged in, ensure that it is tightened properly and not damaged in any way. Note that the cable must be plugged into the COM1 port, not the COM2 port. 2 Check the host computer to ensure that the RS-232 cable is connected to the appropriate COM port. If the cable is plugged in, ensure that it is tightened properly and not damaged in any way. 3 Check the serial port settings (9600, N, 8, 1). If the wrong port or baud rate is selected, change the configuration. For information about changing the software configuration, see Chapter 4, Verifying Installation. USB Cable Problems An option to using RS-232 communications protocol is to use USB protocol. The USB port located on the back of the autosampler is an alternate connection protocol option to the RS-232 port at Com 1. Either RS-232 or USB may be used, but not both at once. 1 Check that the USB cable to ensure it is plugged into the port on the autosampler. 2 Check the host computer to ensure that the USB cable is connected to the appropriate USB port. 3 Check that the USB cable is not damaged in any way. 4 Check the host computer to ensure that the USB drivers are installed for USB operation of the autosampler. Load the proper USB drivers to the host computer from the CD that was provided with the workstation. 72

73 Chapter 7: Troubleshooting the Workstation Software Configuration Problems If the cords are connected properly and the system is still not functioning, ensure that the software is communicating correctly with the workstation. To do so, verify proper operation of all manual functions by completing the following steps: 1 Connect to the workstation using a terminal emulation program (see Operating a CETAC Workstation Using a Terminal Program on page 79). 2 Enter some commands, such as HOME. If the commands have no effect, and a USB connection is being used, check that the workstation is plugged into the same USB port as it was when the driver software was configured. Unlike many consumer devices, each USB port used as a virtual COM port must be individually configured. 3 If manual commands work, but the workstation does not operate with the host software, carefully check that the host software is configured to use the COM port where the workstation is plugged in. If a USB connection is used, and if the manual commands work, but the HOST software fails to operate the workstation, check that the same COM port number is configured in the HOST software and in the USB device driver. 73

74 Chapter 7: Troubleshooting the Workstation Z-Drive Assembly Problems An autosampler malfunction may be caused by a problem in the Z-Drive assembly. You can easily determine that a malfunction is related to the Z-Drive assembly if you hear a loud chattering noise when the autosampler power switch is on or if the sample needle is not moving. To troubleshoot Z-Drive assembly problems, complete the following steps: 1 Check the Y-Axis home position flag for damage. 2 Ensure the sample needle is moving freely. If the sample needle is binding, free the sample needle assembly. NOTE: If you cannot free the Z-Drive assembly, you will need to replace it. See Chapter 3, Installing the Workstation, for information about mounting a new Z-Drive assembly. You can order a new Z-Drive assembly from Teledyne CETAC Technologies. 74

75 Chapter 7: Troubleshooting the Workstation Returning the Product to CETAC for Service Refer to the following information if you need to return the product to Teledyne CETAC Technologies for service. Shipping the Product Follow these guidelines when shipping the product: Contact Teledyne CETAC Technologies before shipping the product. Use the original packing materials. If the original shipping materials are not available, place a generous amount of shock-absorbing material around the instrument and place it in a box that does not allow movement during shipping. Seal the box securely. Pre-pay all shipping expenses including adequate insurance. Write the following information on a tag and attach it to the product: Name and address of the owner Product model number and serial number Description of service required or failure indications The RMA number supplied by CETAC Mark the shipping container as FRAGILE. In all correspondence, refer to the instrument by model name or number and full serial number. Do not return products which are contaminated by radioactive materials, infectious agents, or other materials constituting health hazards to CETAC employees. Product Warranty Statement NOTE Contact Teledyne CETAC Technologies or refer to the warranty card which came with your product for the exact terms of your warranty. The following copy is provided for your convenience, but warranty terms may be different for your purchase or may have changed after this manual was published. TELEDYNE CETAC TECHNOLOGIES warrants automation instruments for (2) two years from the date of shipment. Any CETAC manufactured unit sold directly to the End-User found in reasonable judgment of CETAC to be defective in material or workmanship will be repaired by CETAC without charge for parts and labor. Only CETAC manufactured automation instruments are covered by the (2) two year warranty. For a complete listing of our Automation products please visit our web site at and follow the Automation link. The unit, including any defective part, must be returned to CETAC within the warranty period. The expense of returning the unit to CETAC for warranty service will be paid for by the buyer. CETAC s responsibility in respect to warranty claims is limited to making the required repairs or replacements, and 75

76 Chapter 7: Troubleshooting the Workstation no claim of breach of warranty shall be cause for cancellation or recession of the contract of sale of any unit. Products may not be returned which are contaminated by radioactive materials, infectious agents or other materials constituting health hazards to CETAC employees. This warranty does not cover any unit that has been subject to misuse, neglect, negligence or accident. The warranty does not apply to any damage to the unit that is the result of improper installation or maintenance, or to any unit that has been operated or maintained in any way contrary to the operating or maintenance instructions as specified in the CETAC Instruction and Operations Manual. The warranty does not cover any unit that has been altered or modified so as to change its intended use. Any attempt to repair or alter any CETAC unit by anyone other than by CETAC authorized personnel or agents will void this warranty. In addition, the warranty does not extend to the repairs made necessary by the use of parts, accessories, or fluids which are either incompatible with the unit or adversely affect its operation, performance or durability. CETAC reserves the right to change or improve the design of any unit without assuming any obligation to modify any unit previously manufactured. THE FOREGOING EXPRESS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED INCLUDING WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. CETAC S OBLIGATION UNDER THIS WARRANTY IS STRICTLY AND EXCLUSIVELY LIMITED TO THE REPAIR OR REPLACEMENT OF DE-FECTIVE PARTS, AND CETAC DOES NOT ASSUME OR AUTHORIZE ANYONE TO ASSUME FOR THEM ANY OTHER OBLIGATION. CETAC ASSUMES NO RESPONSIBILITY FOR INCIDENTAL CONSEQUENTIAL OR OTHER DAMAGES (EVEN IF ADVISED OF SUCH POSSIBILITY), INCLUDING BUT NOT LIMITED TO, LOSS OR DAMAGE OF PROPERTY, LOSS OF REVENUE, LOSS OF USE OF THE UNIT, LOSS OF TIME, OR INCONVENIENCE. This warranty and all matters arising pursuant of it shall be governed by the laws of the State of Nebraska, United States. Returned Product Procedures Claims for shipment damage (evident or concealed) must be filed with the carrier by the buyer. CETAC must be notified within ninety (90) days of shipment of incorrect materials. No product may be returned, whether in warranty or out of warranty, without first obtaining approval from CETAC. No replacements will be provided, nor repairs made, for products returned without such approval. Any returned product must be accompanied by a return authorization number. The expense of returning the unit to CETAC for service will be paid by the buyer. The status of any product returned later than thirty (30) days after issuance of a return authorization number will be subject to review. Shipment of repaired products will generally be made forty-eight (48) hours after the receipt. Do not return products which are contaminated by radioactive materials, infectious agents, or other materials constituting health hazards to CETAC employees. 76

77 Chapter 7: Troubleshooting the Workstation Returned Product Warranty Determination After CETAC s examination, warranty or out of warranty status will be determined. If a warranted defect exists, the product will be repaired at no charge and shipped prepaid back to the buyer. If the buyer desires an air freight return, the product will be shipped collect. Warranty repairs do not extend the original warranty period. If an out of warranty defect exists, the buyer shall be notified of the repair cost. At such time the buyer must issue a valid purchase order to cover the cost of repair and freight, or authorize the products to be shipped back as is, at the buyer s expense. Failure to obtain a purchase order number approval within fifteen (15) days of notification will result in the products being returned as is, at the buyer s expense. 77

78 Chapter 7: Troubleshooting the Workstation This page is intentionally blank. 78

79 8 Operating a CETAC Workstation Using a Terminal Program The workstation can be controlled using a serial communications protocol. You can use any terminal emulation program, including: C-Term. This program is installed with the Xpress Configuration Tool software, and runs on Windows 2000 and later. (recommended) This chapter explains how to operate a CETAC autosampler using either of the two programs. Using C-Term The autosampler can be controlled by sending it simple commands. This can be helpful for troubleshooting. You can use any terminal emulation program. This chapter explains how to operate a CETAC autosampler using the C-Term terminal program, which is provided on the software CD. C-Term is a simple terminal program developed to validate the installation and functionality of various CETAC devices. C-Term communicates through a serial (RS-232) port on the host computer. If the autosampler is connected to a USB port, the device driver will create a virtual serial port. Installing C-Term 1 On the included CD, find the C-Term folder. 2 Double-click CTerm_setup.exe. Starting C-Term 1 Check that the autosampler is connected to the PC. 79

80 Chapter 8: Operating a CETAC Workstation Using a Terminal Program 2 Close any programs which might be communicating with the autosampler. 3 On the Start Menu, click All Programs Teledyne CETAC Technologies Support Tools C-Term. Overview of the C-Term Window Copy Paste Clear Terminal Buffer Disconnect Connect COM Port & Status Baud Rate FIGURE 8-1 C-Term Window. Commands typed in this window are sent to the CETAC device connected to the opened port. The typed commands will appear in light green in the terminal buffer. Responses from the autosampler will appear in red. Non-printing characters such as carriage returns will appear as ASCII hexadecimal numbers surrounded by square brackets; for example, [0D] is the carriage return character. Configuring C-Term By default, C-Term attempts to open COM1 the first time it is executed. If the autosampler is connected to another COM port, configure C-Term to use the desired port. 1 On the Tools menu, click Setup Serial Port. 2 Select the desired COM port and click OK. NOTE The autosampler communicates at 9600 baud (which is the default.) Only installed COM ports, including USB virtual COM ports, will appear in the Comm Port menu. Autosampler Commands The following commands are helpful for verifying autosampler operation. This is not a comprehensive list of commands. Command Description 80

81 Chapter 8: Operating a CETAC Workstation Using a Terminal Program VERSS HOME ABS=x-y-z PMP ON PMP OFF SET AUX=1 RES AUX=1 IN=1 Returns firmware version. Returns sample needle to home position. Moves to position defined in steps of 0.1 mm from the home position. Example: ABS= moves to X=100 mm, Y=100 mm, Z=top. Turn the peristaltic pump on. Turn the peristaltic pump off. Sets and clears the output to the ICP-MS Tests the status of the input from the ICP-MS TABLE 8-1 Autosampler Commands CAUTION Make sure that there is enough space for the sample needle to move. Equipment damage can occur if the needle collides with an obstruction such as a vial or rack. 81

82 Chapter 8: Operating a CETAC Workstation Using a Terminal Program This page is intentionally blank. 82

83 Chapter 9: Safety and Regulatory Information 9 Safety and Regulatory Information Review this product and related documentation to familiarize with safety markings and instructions before you operate the instrument. Characteristics Environmental Characteristics Operating Temperature +5 C to +40 C (+41 F to +104 F) Non-Operating Temperature +0 C to +55 C (+32 to +131 F) Operating Altitude Operating Relative Humidity Non-Operating Relative Humidity Up to 2,000 m (6,562 ft) 0% to 80% non-condensing for temperatures up to 31 C, decreasing linearly to 50% at 40 C 0% to 95% non-condensing Pollution Degree Pollution Degree 2 Normally no pollution or only dry, nonconductive pollution occurs. The pollution has no influence. Occasionally, however, a temporary conductivity caused by condensation may be expected. TABLE 9-1: Environmental Characteristics For indoor use only. 83

84 Chapter 9: Safety and Regulatory Information Avoid sudden, extreme temperature changes which could cause condensation on circuit boards in the product. See page 15 for information on chemical compatibility. Electrical Characteristics Power requirements 4-Port Power Supply XYZ Autosampler Valve Syringe Module Input: AC Voltage, Frequency, and Current V ~ Hz 3.6 A at 100 VAC, 1.8 A at 200 VAC Installation Category: CAT II (Line voltage in appliance and to wall outlet) 24 VDC Output: 24 V DC Maximum output current (sum of all connectors): 13 A Connect only to the autosampler and the supplied accessory modules and optional Peltier rack module. Input: DC Voltage and Current 24 V 3.33 A Installation Category: CAT I (Mains isolated) Use only with the provided power supply. Input: DC Voltage and Current Syringe Pump Module 24 V 4 A Installation Category: CAT I (Mains isolated) Use only with the provided power supply. Connect only with the supplied 6-pin power/data cable. Input: DC Voltage and Current TABLE 9-2: Power Requirements 24 V 1 A Installation Category: CAT I (Mains isolated) Use only with the provided power supply. Connect only with the supplied 6-pin power/data cable. 84

85 Chapter 9: Safety and Regulatory Information Data connectors (on autosampler) All terminals allow a maximum current of 28VDC, unless otherwise noted. Note that the USB and COM connectors may not be used at the same time. COM 1 COM 2 USB ETHERNET Z-DRIVE AUXILIARY DB-9 RS-232 serial connection to a controller PC. DB-9 RS-232 serial connection to accessories or instruments. USB connection to a controller PC. Ethernet connection. (Disabled by default.) 15-pin power and signal connection for Z-drive. 24V max output. Connect only to the supplied Z-drive, using the cable which is part of the Z-drive. Additional connectors may be provided for specific applications. The standard auxiliary connector has the following connections: Note 1: Output voltage is selected with an internal jumper. It can be configured to either +5V or +24V DC. TABLE 9-3: Electrical Input and Output Connectors on the Autosampler Data connectors (on power supply) RS232 RS485 RS-232 serial connection to the autosampler or host PC (max ±12 VDC, 8 ma) TIA/EIA-485 (Not used, max ±8VDC, 200µA) TABLE 9-4: Data Connectors on the Power Supply 85

86 Chapter 9: Safety and Regulatory Information Data connectors (on valve syringe module and syringe pump module) RS-485 USB COM TIA/EIA-485 connection to host PC (max ±8 VDC, 200 µa) USB connection to host PC (5VDC) DB-9 RS-232 serial connection to host PC (max ±12 VDC, 8 ma) TABLE 9-5: Data Connectors on the Syringe Modules Battery The autosampler contains a battery. The battery does not need to be replaced for routine operation of the autosampler. CAUTION FIRE AND EXPLOSION HAZARD Replace only with a battery of the same type. Battery description: CR2032 (3.0V, non-rechargeable lithium coin cell) See page 68 for battery replacement instructions. 86

87 Chapter 9: Safety and Regulatory Information Safety Notices WARNING INJURY HAZARD If the equipment is used in a manner not specified by Teledyne CETAC Technologies, the protection provided the equipment may be impaired. Repair or service that this not covered in this manual should only be performed by qualified personnel. Power Cord Set Requirements The power cord set supplied with your instrument meets the requirements of the country where you purchased the instrument. Power is supplied to the autosampler through the included 24V power supply. Power Cord Safety Maintenance The operator should check the power/signal supply cord condition. The equipment should not be operated if the mains inlet is cracked or broken. Any obvious damage to the case (from a drop or fall) should be checked by service personnel for loose or damaged parts. See individual parts lists for approved replacement parts. Mains Disconnect The power switch on the rear panel is not the mains disconnect. Power mains disconnect is accomplished by unplugging the power cord at the power supply or at the wall outlet. Ensure the power cord is easily accessible and removable, in the event of an emergency which requires immediate disconnection. WARNING SHOCK HAZARD Ensure that power cord is disconnected before removal of any covers. 87

88 Chapter 9: Safety and Regulatory Information Cleaning Instructions For additional cleaning information, see cleaning in the index. To clean the exterior surfaces of the instrument, complete the following steps: 1 Shut down and unplug the instrument. 2 Wipe the instrument exterior surfaces only using a towel dampened with a lab-grade cleaning agent. 3 Repeat step 2, using a towel dampened with clear water. 4 Dry the instrument exterior using a dry towel. WARNING SHOCK HAZARD Do not allow any liquid to enter the instrument cabinet other than as intended through the specified tubing, or come into contact with any electrical components. The instrument must be thoroughly dry before you reconnect power, or turn the instrument on. Mechanical Hazards If you insert any part of your body between the moving parts of the workstation, you could be injured. FIGURE 9-1 shows the location of potential hazards FIGURE 9-1 Overview of the Mechanical Hazards of an Autosampler. WARNING 1 - PINCH HAZARD Keep fingers, hair, and loose clothing away from the moving parts of the workstation. 2 - PUNCTURE/INJECTION HAZARD Keep hands away from the sample needle while the workstation is operating. 88