Tutorial for virtual machine creation and installation of Linux C4Sys iso file in Windows.

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1 Tutorial for virtual machine creation and installation of Linux C4Sys iso file in Windows. To start your virtual machine creation it is necessary to download the software: Oracle Virtual Box (follow the website instructions for installation) And please, download the C4sys.iso file from: After the installation, running the Virtual Box program should take you to this screen. Click on New and the following pop-up should appear. 1

2 There are three filds to be completed. (1) The name of your new virtual machine (VM). Here we will choose C4sys. (2) The system type, choose Linux and (3) the version, choose Ubuntu (64-bit). You should get a screen like the next one. After clicking next, the system will ask you for the desired RAM memory size. As shown in the next figure. 2

3 In our example we have 16 GB in the host machine and will dedicate 4 GB to the virtual machine. In this step, we are defining how much memory will last to the host machine when the virtual machine is running. So, it is normal to experience some reduced performance while running the VM. Select at least 2 GB or RAM for the VM (ideally 4) and click next. Therefore, choose the option create a virtual hard disk. 3

4 Choose the VDI disk file type and click Next. Choose fixed size and click Next. 4

5 The software will ask you the amount of hard disk (HD) memory to allocate for the VM. We need at least 30 GB to run all the software required for genome assembly. Change the size to 30 GB and click on Create. If everything went well you will see a progress bar like this. 5

6 Now, your Virtual Machine for Linux is ready, but you still do not have an Operating System (OS). To install the C4sys Ubuntu Distro, you will need the C4sys.iso file that can be downloaded from here: Make sure you download the file, click on the Settings and start configuring your VM booting system. Clicking on Settings should open a window like this. Now, click on System in the lateral menu and select the tab Processor. 6

7 In our example, we have 8 processors in the host machine and we are defining 4 processors for the VM. One processor is enough to handle the genome assembly, but if you assign more than one you will have a nicer experience. Keep in mind that you are sharing the processors of your host machine, so all the ones you assign to the VM will be unavailable for your host machine during the use of the VM. Considering this, set as many as you like to use the C4sys Distro and click on Storage in the side menu. After clicking on Storage you will see a screen like this. Here you will need to define the booting device. To do that, click on Empty under the Controller: IDE in the Storage Tree and on the small disk icon on the right side of the image, as in the following figure. 7

8 Click on the option Choose Virtual Optical Disk File and a window will pop-up allowing you to search for the C4sys.iso file. Navigate to the directory where you downloaded your file, choose the C4sys.iso file and click Open. 8

9 Click on OK and you are ready to start your VM. Double click on your newly created machine to turn it on. 9

10 Wait a few seconds until the login window to show up, select the C4sys user, type the password c4sys and hit enter. This is the basic distro interface but remember that it is running from the.iso file, which is a plug-and-play device, this means that changes made in this device will not be stored. To be able to store modifications, we need to install this OS in our VM. Double click on the install icon in your desktop. 10

11 This action will open a terminal and will ask for a password. Type the login password c4sys and wait for the installation screen to appear. #From now on, it is a normal guided installation of the Linux system. Now it is going to start a basic Linux system installation. Choose the language and click continue. In order to keep the standardization of directory names and commands, we recommend to choose English language. 11

12 This procedure will ask if the user wants to download updates while running the installation. We recomment to keep the boxes unchecked and click Continue. This screen might look scary when it asks if you want to erase the whole disk. Don t worry, the VM cannot see outside of the 30 GB of HD memory we defined before, remember that this machine is virtual, so there is no risk of erasing the host machine files. Keep the first option marked and click on Install Now. 12

13 It will ask for your region to adjust the clock and location settings. Select the country of your preference and click Continue. Choose your keyboard language configuration, click Continue and wait the installation to finish. 13

14 You will see a loading bar and a message of finished installation at the end. When you see the message, click on Restart Now and your virtual machine will be ready to use. 14

15 Hit Enter on this screen and your system will restart. Now your Linux C4sys Distro is installed and you are ready for the class. Congratulations! See you in Februrary. Daniel K. Morais - daniel.morais@biomed.cas.cz Laboratory of Environmental Microbiology Microbiology Institute Czech Academy of Sciences 15

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