Since the introduction of the first commercial

Transcription

1 BR-9742C

2 Since the introduction of the first commercial ultracentrifuge in 947 the classic Beckman Model L Beckman Coulter has been at the forefront of centrifuge innovation. Although the physics of this basic separation technique never change, Beckman Coulter continually designs new and innovative rotors and accessories, and develops advanced methods that allow the forces of centrifugation to be applied in new ways. This centrifuge product selection guide is designed to help you determine the most efficient centrifuge tools for your laboratory. Each section begins with a brief description of instruments that Beckman Coulter offers within that cen tri fuge category. Because biocontainment is a major concern in today s laboratories, Beckman Coulter provides a number of options that address this issue. Special biocontainment accessories are available across our centrifuge product line and are identified with this icon: Biosafety Rotors with this symbol have been tested* to demonstrate containment of aerosols under normal operating conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed. Following the cen tri fuge descriptions, listings of their rotors are included with in for mation on speed and g-force capability. Also included is in for mation on tubes and bottles that can be used and the adapters they require. Tubes and bottles are cross-referenced in a separate section that provides details on tube materials, chemical com patibil ity, tube designs, and tube closure options. A reference section at the back of the guide includes quick-reference charts on instrument and tube selection and frequently-used formulas, as well as a listing of centrifuge lit era ture and training tools available from Beckman Coulter. * The rotors were tested for microbiological containment at an independent, third-party testing facility 2 and found to be suitable for use with materials up to ACDP category. 3 Improper use or maintenance may affect seal integrity and thus containment. Testing by these facilities does not imply their endorsement of these products.. Harper, G. J. (984) Evaluation of sealed containers for use in centrifuges by a dynamic mi cro biological method. J. Clin Pathol. 37, CAMR, Porton Down, U.K., or USAMRIID, Fort Detrick, Maryland, U.S.A. 3. Advisory Committee on Dangerous Pathogens (984). Categorization of pathogens according to hazard and categories of containment. HMSO, ISBNO Contact your local Beckman Coulter office Australia, Gladesville (6) Canada, Mississauga () China, Beijing (86) Czech Republic, Prague (420) Eastern Europe, Middle East, North Africa, South West Asia: Switzerland, Nyon (4) France, Villepinte (33) Germany, Krefeld (49) Hong Kong (852) India, Mumbai (9) Italy, Cassinadé Pecchi (Milan) (39) Japan, Tokyo (8) Seoul, Korea (82) Latin America () (305) Mexico, Mexico City (00) Netherlands, Mijdrecht (3) Puerto Rico (787) Singapore (65) South Africa/Sub-Saharan Africa, Johannesburg (27) /5 Spain, Madrid (34) Sweden, Bromma (46) Switzerland, Nyon (4) Taiwan, Taipei (886) Turkey, Istanbul (90) UK, High Wycombe (44) USA, Fullerton, CA () Additional centrifugation resources: i

3 Table of Contents Benchtop Centrifugation Allegra X-5R Series Centrifuges...-2 Allegra X-2 Series Centrifuges...-3 Allegra X-22 Series Multipurpose Centrifuges...-4 Allegra 25R High-Performing Centrifuge...-5 Allegra 64R Centrifuge...-6 Benchtop Centrifuges Rotor Summary...-7 Fixed-Angle Rotors...-0 Horizontal Rotor Swinging-Bucket Rotors Microcentrifugation Microfuge 6 Centrifuge Microfuge 8 Centrifuge Microfuge 22R Centrifuge Microfuge Centrifuges Rotor Summary Fixed-Angle Rotors Swinging-Bucket Rotor Tubes and Bottles Tubes and Bottles for Every Application...3- Tube Selection Considerations Compatibility of Tube Material with Solvents and Sample Gradient Formation and Fractionation High-Temperature Centrifugation Tube Sizes Tube Cleaning, Sterilization, and Reuse Tube Closures Quick Reference Chart to Tube Materials and Their Properties General Filling and Sealing Requirements Bottles Used in Benchtop Centrifuges Adapters for Non-Beckman Coulter Tubes and Bottles Adapters for Glass Tubes in Beckman Coulter Rotors Tubes and Bottles (Listed by Volume) Tools and Supplies Tools and Supplies Reference Guide to Centrifuge Selection Useful Formulas Support Services On-Call Support On-Line Support On-Site Support On-File Support ii

4 Benchtop Centrifugation Allegra X-5R Series Centrifuges...-2 Allegra X-2 Series Centrifuges...-3 Allegra X-22 Series Multipurpose Centrifuges...-4 Allegra 25R High-Performing Centrifuge...-5 Allegra 64R Centrifuge...-6 Benchtop Centrifuges Rotor Summary...-7 Fixed-Angle Rotors...-0 F F F2402H...-2 F F F F F F TA FX TA FX TA C C Horizontal Rotor H Swinging Bucket Rotors SX S TS SX SX4750 / SX4750A...-3 S S

5 Benchtop Centrifugation Allegra X-5R Benchtop Centrifuge Offering new power and flexibility on the bench, the Allegra X-5R provides you with faster separations, optimized recoveries, and time savings. Allegra X-5R Centrifuge with Cell Culture Flask Adapters * Achieve Up to 5,250 x g in your 4 x 750-mL Swinging-Bucket Rotor Optimize recoveries Faster separations Save time Perfect for: Filtration and concentration Fast and slow blood component spins Microbial cell harvesting Directly centrifuge your cell culture flasks * Save time and labor Eliminate 5-mL or 50-mL conical tubes Reduce a potential con tami nation step Complete your run even with im balance ARIES Smart Balance Rotor automatically corrects for imbalance up to 50 grams opposing loads Enjoy the convenience of a truly inno vative interface Numerical keypad Programmable: Stores up to ten user-defined programs Part Numbers Allegra X-5R (Refrigerated) 60 Hz, 208 V Hz, 230 V /60 Hz, 200 V Specifications Maximum Speed rpm/4 750 rpm Maximum g-force 400 x g/5 250 x g Speed Control ± 0 rpm of set speed Drive Type Brushless induction Time Setting Set (up to 99 hours 59 minutes), Hold, or Pulse (short run) User-Defined Programs 0 Accel/Decel Rates 0 accel, decel Temperature Range -0 to +40 C Dimensions 34.3 cm (3.5 in) H x 62.2 cm (24.5 in) D x 76.2 cm (30 in) W Weight 28 kg (283 lb) * Adapters (patent pending) currently available for Corning 75cm2 and 25cm2 canted neck cell culture fl asks. Corning is a trademark of Corning Incorporated. Using fi xed angle rotors / Using swinging bucket rotors. All rotors and carriers with covers are certifi ed by CAMR, Porton Down, UK, for Biocontainment. -2

6 Benchtop Centrifugation Allegra X-2 Series Benchtop Centrifuges Simplify your processes and save time with the Allegra X-2 Series Benchtop Centrifuges. Directly centrifuge your cell culture flasks * Save time and labor Eliminate 5-mL or 50-mL conical tubes Reduce a potential con tami nation step Complete your run even with im balance ARIES Smart Balance Rotor automatically corrects for imbalance up to 50 grams opposing loads Enjoy the convenience of a truly inno vative interface Numerical keypad Programmable: Stores up to ten user-defined programs Allegra X-2 Centrifuge Part Numbers Allegra X-2R Allegra X-2 (Refrigerated) (Constant Controlled Temperature) 60 Hz, 208 V Hz, 230 V /60 Hz, 200 V Specifications Maximum Speed rpm/3 750 rpm Maximum g-force 400 x g/3 270 x g Speed Control ± 0 rpm of set speed Drive Type Brushless induction Time Setting Set (up to 99 hours 59 minutes), Hold, or Pulse (short run) User-Defined Programs 0 Accel/Decel Rates 0 accel, decel Temperature Range -0 to +40 C (selectable) for Allegra X-2R; 8 to 22 C (preset) for Allegra X-2 Dimensions Allegra X-2 and X-2R Weight Allegra X-2 and X-2R 34.3 cm (3.5 in) H x 62.2 cm (24.5 in) D x 76.2 cm (30 in) W 2 kg (267 lb) * Adapters (patent pending) currently available for Corning 75cm2 and 25cm2 canted neck cell culture fl asks. Corning is a trademark of Corning Incorporated. Using fi xed angle rotors / Using swinging bucket rotors. -3

7 Benchtop Centrifugation Allegra X-22 Series Compact Multipurpose Benchtop Centrifuges The compact Allegra X-22 Series is up to 25 cm (0 inches) thinner than other comparable benchtop centrifuges. With a library of eleven rotors, the Allegra X-22 Series is the spacesaving multipurpose solution for your requirements. Specifications Allegra X-22R Centrifuge Maximum Speed rpm/4 500 rpm* Maximum g-force x g/9 309 x g* Drive Type Brushless induction Time Setting Timed up to 9 hr 59 min, pulse (short run), hold Accel/Decel Rates 0 accel, 0 decel Settable Temperature Range -20 to +40 C/NA Operating Temperature Range 2 to +40 C/Ambient Dimensions Allegra X cm (4 in) H x 55 cm (2.7 in) D x 46 cm (8. in) W Allegra X-22R 37 cm (4.6 in) H x 70.7 cm (27.8 in) D x 46 cm (8. in) W Weight (without Rotor) Allegra X kg (06 lb) Allegra X-22R 78 kg (72 lb) Part Numbers Allegra X-22 Allegra X-22R Benchtop Refrigerated Benchtop 60 Hz, 20 V Hz, 230 V /60 Hz, 00 V * Using fi xed angle rotor in Allegra X-22R/Using fi xed angle rotor in Allegra X

8 * Benchtop Centrifugation Allegra 25R High-Performing Centrifuge The Allegra 25R High-Performing Benchtop Centrifuge is perfect for DNA from bacteria and yeast pelleting through isolation and purification to post-reaction cleanup. With a wide range of interchangeable swinging bucket and fixed angle rotors, it accommodates from 0.25 ml to 500 ml, including popular.5 ml tubes for mini-preps and spin columns; 50 ml, 250 ml, and 500 ml tubes; 96-well plates; DNA kits; and PCR plates. With a maximum rpm (25 60 x g), you can complete DNA sample prep quickly and efficiently before moving on to the rest of your DNA process. Specifications Part Numbers Allegra 25R Centrifuge Allegra 25R (Refrigerated) 60 Hz, 208 V /60 Hz, 200 V Hz, 230 V Maximum Speed Maximum g-force Speed Control Drive Type Time Setting Accel/Decel Rates Settable Temperature Range Operating Temperature Range Maximum Heat Output Maximum Noise Output Dimensions Weight rpm x g ± 50 rpm of set speed Brushless induction 9 hr 59 min timer, hold; pulse (short run) 0 independent profi les -20 to +40 C 2 to +40 C Btu/h (2.0 kw) < 68 db 40.5 cm (6 in) H x 68.5 cm (27 in) D x 65.0 cm (25.6 in) W 24.0 kg (273.4 lb) -5

9 Benchtop Centrifugation Allegra 64R Centrifuge The Allegra 64R takes high g-forces (up to x g) to the lab bench. Optimal for subcellular fractionation, proteins, and viruses you can spin from 0.25 ml to 85 ml; operate at temperatures from 2 to 40 C; and select rotors that have been certified by Porton Down, UK, for biocontainment. Specifications Part Numbers Allegra 64R Centrifuge Allegra 64R (Refrigerated) 60 Hz, 208 V Hz, 200 V Hz, 200 V Hz, 230 V (European Version) 50 Hz, 230 V (UK Version) Maximum Speed Maximum g-force Speed Control Drive Type Time Setting Accel/Decel Rates Settable Temperature Range Operating Temperature Range Maximum Heat Output Maximum Noise Output Dimensions Weight rpm x g ± 50 rpm of set speed Brushless induction 9 hr 59 min timer, hold 0 independent profi les -20 to +40 C 2 to +40 C Btu/h (.58 kw) 65 dba 38 cm (5.0 in) H x 66 cm (26.0 in) D x 6 cm (24.0 in) W 86 kg (90 lb) -6

10 Benchtop Centrifugation Benchtop Centrifuges Rotor Summary Rotor Part Maximum Maximum Maximum k Factor Number Tubes/Bottles Rotor Approx. Rotor Biosafe Type Number Speed Force (g) Force (g) and Size Capacity Accel. Angle (rpm) at r min at r max (diameter x length) (ml) Time* mm / in. (min:sec) Allegra X-5R Allegra X-2 Series Allegra X-22 Series Allegra 25R Allegra 2 Series Allegra 64R Fixed-Angle Rotors F x :30 45 No 3 mm 64 mm x x.8 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. F x :00 30 No 23 mm 78 mm 25.3 x 92 x 4 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. F2402H x :0 45 Yes 50 mm 82 mm x x.8 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. F x :40 35 No 20 mm 76 mm 6. x x 3 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. F x :00 45 No 57 mm 87 mm x x.8 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. F x :45 25 No 2 mm 84 mm 29 x x 4.75 Density gradient separations of erythrocytes, cell lysate fractions, granules; differential separation of DNA, proteins, and viruses. F x :5 30 No 2 mm 90 mm 38 x 04.5 x 4 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. F x :00 25 No 33 mm 94 mm 29 x x 4 Density gradient separations of erythrocytes, cell lysate fractions, granules; differential separation of DNA, proteins, and viruses. F x :45 25 No 25 mm 97 mm 38 x 04.5 x 4 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. TA x :34 45 Yes 67 mm 00 mm x x.8 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. FX ** x :00 45 No 67 mm 00 mm x x.8 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Biosafety Biosafety * Acceleration times are approximate and are subject to change. The Allegra 2 Series has been made obsolete. Typical acceleration time in Allegra 64R using accel curve 9. ** In refrigerated centrifuges; maximum speed/rcf values in nonrefrigerated centrifuges are lower. Refer to Rotor Manual. Typical acceleration time in Allegra 25R centrifuge. -7

11 Benchtop Centrifugation Benchtop Centrifuges Rotor Summary (cont d) Rotor Part Maximum Maximum Maximum k Factor Number Tubes/Bottles Rotor Approx. Rotor Biosafe Type Number Speed Force (g) Force (g) and Size Capacity Accel. Angle (rpm) at r min at r max (diameter x length) (ml) Time* mm / in. (min:sec) Allegra X-5R Allegra X-2 Series Allegra X-22 Series Allegra 25R Allegra 2 Series Allegra 64R Fixed-Angle Rotors (cont d) FX x :30 25 Yes 35 mm 98 mm 38 x 02 Biosafety.5 x 4 Density gradient separations of erythrocytes, cell lysate fractions, granules; differential separation of DNA, proteins, and viruses. TA x :37** 45 No 33 mm 96 mm 29 x x 4 Pellet cells from large volumes or cell particles from tissue homogenates. Short-column methods used to purify large quantities of virus in gradients. TA , ,450 6 x L 2:00** 45 No 35 mm 37 mm 62 x x 5.75 Pellet cells from large volumes or cell particles from tissue homogenates. Short-column methods used to purify large quantities of virus in gradients. C x :55* 25 No 38 mm 93 mm 7 x x 4.75 Pellet subcellular organelles,viruses, bacteria, mitochondria, chloroplasts, or algae. C x :00* 25 No 32 mm 92 mm 28.5 x x 4.25 General pelleting of cells, bacteria, and food products; separation of proteins, viruses, and subcellular fractions; phase separations; binding studies. Horizontal and Swinging Bucket Rotors SX x :37 90 No 35 mm 74 mm x x.8 Pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. H x :25* 90 Yes 35 mm 94 mm x 38 Biosafety 0.5 x.5 Pellet subcellular organelles, viruses, bacteria, mi to chon dria, chloroplasts, or algae. S x :23* 90 No 26 mm 96 mm 6 x x 3.25 Sediment protein precipitates, large particles, cells, cell debris, and for separationsusing gradients. TS x L :00 90 Yes 87 mm 90 mm 85 x x 5.5 Sediment protein precipitates, large particles, cells, and cell debris. Biosafety * Acceleration times are approximate and are subject to change. Allegra 2 Series has been made obselete. In refrigerated centrifuges; maximum speed/rcf values in nonrefrigerated centrifuges are lower. Refer to Rotor Manual. ** Typical accel time in Allegra 2 Series, accel curve 9, rotor fully loaded. -8

12 Benchtop Centrifuges Rotor Summary (cont d) Rotor Part Maximum Maximum Maximum k Factor Number Tubes/Bottles Rotor Approx. Rotor Biosafe Type Number Speed Force (g) Force (g) and Size Capacity Accel. Angle (rpm) at r min at r max (diameter x length) (ml) Time* mm / in. (min:sec) Allegra X-5R Allegra X-2 Series Allegra X-22 Series Allegra 25R Allegra 2 Series Allegra 64R Horizontal and Swinging Bucket Rotors (cont d) SX x 250 L 0:36 90 No 7 mm 72 mm 6.6 x x 4.9 Sediment protein precipitates, large particles, cells, and cell debris. SX NA x L 0:80 90 Yes mm 96 x 30 Biosafety 3.85 x 5.25 Rapid sedimentation of protein precipitates, large particles, cells, and cell debris. SX4750A NA x L 0:80 90 Yes mm 96 x x 5.25 Rapid sedimentation of protein precipitates, large particles, cells, and cell debris. Biosafety S plate carriers, 576 0:2** 90 No 70 mm 0 mm 6 plates, 2 deepwell Serial dilution of liquid volumes. S microplates, 960 0:47 90 No 38.6 mm 68.5 mm 4 deepwell 2 96-well kits 2 PCR plates High-throughput DNA sample preparation; serial dilution of small liquid volumes. * Acceleration times are approximate and are subject to change. Allegra 2 Series centrifuges discontinued. In refrigerated centrifuges; maximum speed/rcf values in nonrefrigerated centrifuges are lower. Refer to Rotor Manual. ** Typical accel time in Allegra 2 Series, accel curve 9, rotor fully loaded. In Allegra X-2 Series centrifuges, maximum speed and g-force are RPM and x g, respectively. -9

13 F202 2 x.8 ml F202 Axis of Rotation r min (3 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular or ganelles, DNA, viruses, bacteria, mitochondria, chlo ro plasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size r av (47.5 mm) r max (64 mm) x.8 ml 2.6 ml x 45 mm 0.44 x.8 in For use in the Allegra 64R centrifuge. No F202 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Open Top Tubes, Plain Polyethylene 250 μl x μl x Open Top Tubes, Coated Polyethylene Heparin/Lithium/Fluoride coated 250 μl x Heparin/Lithium coated 250 μl x Heparin/Lithium/Fluoride coated 400 μl x Heparin/Lithium coated 400 μl x Tubes with Attached Caps Polyallomer x Polyethylene x Tubes with Separate Caps Polypropylene 400 μl x x Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ μl Rotor Re placement Parts Rotor Lid 9693 Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench Adapters

14 F x 38.5 ml F0630 Axis of Rotation r min (23 mm) r av (50.5 mm) r max (78 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting sub cellu lar organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size * x 38.5 ml 23 ml 25 x 00 mm x 4 in 30 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No F0630 Fixed-Angle Rotor, for rpm * operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polycarbonate x Bottles with Screw Caps Polyallomer x Polycarbonate x Open Top Tubes Polyallomer, Thin-wall x Polyallomer, Thick-wall x Ultra-Clear x * In the Allegra 64R centrifuge. In the Allegra X22, the maximum rpm is and the maximum g-force is In the Allegra X-22R, the maximum rpm is and the maximum g-force is This rotor is rated at a lower speed in the Allegra 2 and 2R centrifuges, so g-forces and k factors are different. See the rotor manual for specifi c information. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench -

15 F2402H Biosafety 24 x.8 ml F2402H Axis of Rotation 45 Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size rmin (50 mm) rav (65 mm) r max (80 mm) a x.8 ml 43.2 ml x 45 mm 0.44 x.8 in For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No F2402H Hermetically Sealed Biosafety Rotor, for rpm a operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Open Top Tubes, Plain Polyethylene 250 μl x μl x Open Top Tubes, Coated Polyethylene Heparin/Lithium/Fluoride coated 250 μl x Heparin/Lithium coated 250 μl x Heparin/Lithium/Fluoride coated 400 μl x Heparin/Lithium coated 400 μl x Tubes with Attached Caps Polyallomer x Polyethylene x Tubes with Separate Caps Polypropylene 400 μl x x Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ μl a In the Allegra 64R centrifuge. In the Allegra X22, the maximum rpm is and the maximum g-force is In the Allegra X-22R, the maximum rpm is and the maximum g-force is This rotor is rated at a lower speed in the Allegra 2 and 2R centrifuges, so g-forces and k factors are different. See the rotor manual for specific information. Rotor Re placement Parts Rotor Lid O-ring (small) Tie-down Screw 3637 T-handle Rotor Wrench Rotor O-ring (large) Rotor Lid Adapters

16 F00 0 x 0 ml F00 Axis of Rotation r min (20 mm) r av (48 mm) r max (76 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size * x 0 ml 00 ml 6. x 8. mm x 3 in 35 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No F00 Fixed-Angle Rotor, for rpm * operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Bottles with Screw Caps Polyallomer x Polycarbonate x Teflon x Tubes Polyallomer, Thick-wall x x Polycarbonate x x Ultra-Clear x * In the Allegra 64R centrifuge. This rotor is rated at rpm/9 926 x g in the Allegra X-22R and at rpm/7 896 x g in the Allegra X-22. Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. To facilitate tube removal, place rubber pad (part number ) at the bottom of the tube cavity before inserting the tube. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench -3

17 F x.8 ml F3602 Axis of Rotation r min (47 mm) r av (62 mm) r max (77 mm) Axis of Rotation r min (57 mm) 45 Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Outer/Inner Outer/Inner Volume/Size / 224/ x.8 ml 64.8 ml x 45 mm 0.48 x.8 in r av (72 mm) r max (87 mm) For use in Allegra 64R and discontinued Allegra 2 Series centrifuges. 45 No F3602 Fixed-Angle Rotor, for rpm a operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Outer/Inner Outer/Inner Speed Tube (ml) Tubes with Attached Caps Polyallomer x / / Polyethylene x / / Tubes with Separate Caps Polypropylene 250 μl x / / μl x / / x / / Tubes, Plain Polyethylene 250 μl x / / μl x / / Tubes, Coated Polyethylene 250 μl 65282* x / / μl x / / μl * x / / μl x / / Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ μl a In the Allegra 64R centrifuge. This rotor is rated at a lower speed in the Allegra 2 and 2R centrifuges, so g-forces and k factors are different. See the rotor manual for specifi c information. * Heparin-Lithium Fluoride coated. Heparin-Lithium coated. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench 3667 Lid Screw 3668 Lid Handle Adapters

18 F x 50 ml F0650 Axis of Rotation r min (2 mm) r av (52.5 mm) r max (84 mm) 25 Fixed-Angle Rotor, Aluminum Major applications: Density gradient separations of erythrocytes, cell lysate fractions, granules, as well as different separations of DNA, proteins, and viruses. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size x 50 ml 300 ml 29 x 07 mm.25 x 4.25 in For use in the Allegra 64R centrifuge. No F0650 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polyallomer x x Polycarbonate x Bottles with Screw Caps Polyallomer x Polycarbonate x Teflon * with High-speed x Screw Cap Tubes Polycarbonate x Polycarbonate, Graduated x Polypropylene x Tubes with Snap-On Caps Polycarbonate x Polypropylene x * Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench -5

19 F x 85 ml F0485 Axis of Rotation r min (2 mm) r av (55.5 mm) r max (90 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size x 85 ml 340 ml 38 x 04 mm.5 x 4 in 30 For use in the Allegra 64R centrifuge. No F0485 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polycarbonate ** 6 38 x x Bottles with Screw Caps Polyallomer 50.0* x Polycarbonate x Polypropylene with Special Cap x Tubes Polyallomer, Thick-wall x Polyallomer, Thin-wall x Polycarbonate x x Polypropylene x * Run with reduced fi ll volume to prevent spilling/leaking. To facilitate tube removal, place rubber pad (part number 34420) at the bottom of the tube cavity before inserting the tube. ** Requires adapter pad Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench Adapters

20 F x 50 ml F0850 Axis of Rotation r min (33 mm) r av (63.5 mm) r max (94 mm) Fixed-Angle Rotor, Aluminum Major applications: Density gradient separations of erythrocytes, cell lysate fractions, granules, as well as different separations of DNA, proteins, and viruses. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 6 500* x 50 ml 400 ml 29 x 04 mm.25 x 4 in 25 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No F0850 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Bottles with Cap Assemblies Polyallomer x x Polycarbonate x Bottles with Screw Caps Polyallomer x Polycarbonate x Teflon with High-speed x Screw Cap Tubes Polycarbonate x Polycarbonate, Graduated x Polypropylene x Tubes with Snap-On Caps Polycarbonate x Polypropylene x * In the Allegra 64R centrifuge. This rotor is rated at rpm/0 528 x g in the Allegra X-22R and at rpm/8 528 x g in the Allegra X-22. Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench Adapters

21 F x 85 ml F0685 Axis of Rotation r min (25 mm) r av (6 mm) r max (97 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 5 500* x 85 ml 50 ml 38 x 04 mm.5 x 4 in 25 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No F0685 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Bottles with Cap Assemblies Polycarbonate x x Bottles with Screw Caps Polyallomer x Polycarbonate x Polypropylene x ** x Tubes Polyallomer, Thick-wall x Polyallomer, Thin-wall x Polycarbonate x x Polypropylene x * In the Allegra 64R centrifuge. This rotor is rated at rpm/0 864 x g in the Allegra X-22R and at rpm/6 953 x g in the Allegra X-22. Requires adapter pad ** Run with reduced fi ll volume to prevent spilling/leaking. To facilitate tube removal, place rubber pad (part number 34420) at the bottom of the tube cavity before inserting the tube. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench Adapters

22 TA-5-.5 Biosafety * 30 x.5 ml TA-5-.5 Axis of Rotation 45 Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts, or algae mm 83.5 mm 00.0 mm rmin rav rmax Max. RPM Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) x.5 ml 0:34/0:50 x 45 mm 0.5 x.8 in. For use in the Allegra 25R centrifuge. No TA-5-.5 Fixed Angle Biosafety Rotor. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Tubes with Snap-on Caps Polyallomer Natural x Polyethylene 250 μl x μl x μl x μl x μl x μl x x Polypropylene Natural x Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ μl * With sealed rotor lid. Heparin-Lithium-Fluoride coated. Heparin-Lithium coated. Rotor Replacement Parts Lid Assembly O-ring Tie-down Screw T-handle Rotor Wrench Adapters

23 FX x 2.2 ml FX r min (67 mm) r av (84 mm) r max (00 mm) Axis of Rotation Fixed-Angle Rotor, Material Major applications: Pelleting, subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 4 000* x 2.2 ml 66 ml x 45 mm 0.48 x.8 in For use in Allegra X-22 Series centrifuges. No FX30.5 Fixed-Angle Rotor Assembly. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapter Adapter Speed Tube (ml) Tubes with Snap-on Caps Polyallomer Natural, Attached cap x * Polyethylene Heparin-Lithium-Fluoride Coated 250 μl x Heparin-Lithium Coated 250 μl x Plain 250 μl x Heparin-Lithium-Fluoride Coated 400 μl x Heparin-Lithium Coated 400 μl x Plain 400 μl x Attached cap x Polypropylene Natural, Separate Cap x * 500- to 750-μL Tubes** 500 μl/ μl * In refrigerated centrifuges; maximum speed in unrefrigerated centrifuges is rpm. In refrigerated centrifuges; g-force at r max in unrefrigerated centrifuges is 9 55 g. No adapter is required with this tube; however, adapter (pkg. of 2) can be used for proper conical support at higher speeds. ** Commercially available. Rotor Re placement Parts Rotor Lid Assembly Lid O-ring Rotor O-ring Tie-down Screw T-handle Rotor Wrench Adapters

24 TA x 50 ml TA-4-50 Axis of Rotation 25 Fixed-Angle Rotor, Aluminum Major applications: Pelleting cells from large volumes, or cell particles from tissue homogenates. Short-column methods (such as partially filled tubes) may also be used to purify large quantities of virus in a cushion gradient. Max. RPM Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) 33.0 mm 64.5 mm 96.0 mm rmin rav rmax x 50 0:37/0:59 29 x 04 mm.25 x 4 in. For use in the Allegra 25R centrifuge. No TA-4-50 Fixed Angle Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polyallomer x x Polypropylene x Bottles with Screw Caps Polyallomer x Polycarbonate x Teflon with High-speed x Screw Cap Tubes Polycarbonate x Polycarbonate, Graduated x Polypropylene x Tubes with Snap-On Caps Polycarbonate x Polypropylene x Rotor Replacement Parts Lid Assembly O-ring Tie-down Screw T-handle Rotor Wrench -2

25 FX600 Biosafety 6 x 00 ml FX r min (35.0 mm) (66.5 mm) (98.0 mm) Fixed-Angle Rotor, Aluminum Major applications: Density gradient separations of erythrocytes, cell lysate fractions, granules; differential separation of DNA, proteins, and viruses. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x 00 ml :30/:20 38 x 02 mm.5 x 4 in r av r max Axis of Rotation For use in Allegra X-2 Series centrifuges. No FX600 Fixed Angle Biosafety Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Screw Caps Polyallomer x Polycarbonate x Polypropylene x x Bottles with Cap Assemblies Polycarbonate x x Tubes with Snap-on Caps Polyallomer x ** Polyethylene x ** Polypropylene x ** Tubes Polyallomer, Thick-wall * x Polyallomer x Polycarbonate x x x x * x Polyethylene x Polypropylene x x Stainless Steel x * To facilitate tube removal, place rubber pad (part number 34420) at the bottom of the tube cavity before inserting the tube. **Can be double stacked to be used inside Rotor Replacement Parts Lid Assembly Large O-ring 0079 Small O-ring -22

26 TA x 250 ml TA Axis of Rotation 25 Fixed-Angle Rotor, Aluminum Major applications: Pelleting cells from large volumes, or cell particles from tissue homogenates. Short-column methods (such as partially filled tubes) may also be used to purify large quantities of virus in a cushion gradient. Max. RPM Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) 35 mm rmin x 250 2:00/2:00 62 x 4 mm 2.5 x 5.75 in. 86 mm 37 mm rav rmax For use in the Allegra 25R centrifuge. No TA Fixed Angle Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polyallomer x x Polypropylene x Polypropylene, Wide-mouth x Polycarbonate, Wide-mouth x Bottles with Screw Caps Polyallomer x Polycarbonate x Tubes with Snap-On Caps Polycarbonate x Polypropylene x Conical Tubes Polycarbonate with Cap x Polypropylene with Cap x Open-Top Tubes Polycarbonate x Polypropylene x Rotor Replacement Parts Tie-down Screw T-handle Rotor Wrench Adapters

27 C05 0 x 5 ml C05 Axis of Rotation r min (38 mm) r av (65.5 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting of subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size * x 5 ml 50 ml 7 x 20 mm 0.65 x 4.75 in r max (93 mm) 25 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No C05 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Tubes Conical x * In the Allegra 64R centrifuge. This rotor is rated at rpm/9 299 x g in the Allegra X-22R and at rpm/8 346 x g in the Allegra X-22. Tubes available from scientifi c supply ven dors. See chart on page 2 6 for adapters used with non-beckman Coulter tubes and bottles. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench -24

28 C x 50 ml C0650 Axis of Rotation r min (32 mm) r av (62 mm) r max (92 mm) Fixed-Angle Rotor, Aluminum Major applications: General pelleting of cells, bacteria, and food products; separation of proteins, viruses, and subcellular fractions; phase separation; and binding studies. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size * x 50 ml 300 ml 28.5 x 07 mm.25 x 4.25 in 25 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 2 Series centrifuges. No C0650 Fixed-Angle Rotor, for rpm operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Tubes Conical x * In the Allegra 64R centrifuge. This rotor is rated at rpm/9 420 x g in the Allegra X-22R and at rpm/8 420 x g in the Allegra X-22. Tubes available from scientifi c supply vendors. See chart on page 2 6 for adapters used with non-beckman Coulter tubes and bottles. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw 3637 T-handle Rotor Wrench -25

29 H6002 Biosafety 60 x.8 ml H6002 Axis of Rotation r min (35 mm) r av (64.5 mm) r max (94 mm) Horizontal (Bowl) Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size * N.A. 60 x.8 ml 08 ml x 39 mm 0.44 x.5 in 90 For use in Allegra 64R and discontinued Allegra 2 Series centrifuges. No H6002 Horizontal Biosafety Rotor, for rpm operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Tube Holder Tubes per g-force k Factor Maximum Volume per (mm) Color & Part No. Holder Speed Tube (ml) Set of 2/Set of 6 Tubes with Attached Caps Polyallomer x 38 blue / Polyethylene x 39 blue / Tubes Microtainer ** 600 μl red red Polypropylene 400 μl x 40 dark green dark green μl x 28 red red x 38 blue / Plain Tubes Polyethylene 250 μl x 45 dark green dark green μl x 40 dark green dark green Coated Tubes Polyethylene Yellow 250 μl x 45 dark green dark green Polyethylene Blue 250 μl x 45 dark green dark green Polyethylene Yellow 400 μl x 40 dark green dark green Polyethylene Blue 400 μl x 40 dark green dark green * In the Allegra 64R centrifuge. This rotor is rated at a lower speed in the Allegra 2 and 2R centrifuges, so g-forces and k factors are different. See the rotor manual for specifi c information. Bold numbers feature 30 resting angle to minimize remixing of loose pellets upon completion of run. Based on empirical tests of tube strength with the lid in place on the rotor. ** Microtainer is a registered trademark of Becton, Dickinson and Company; available commercially. Rotor Re placement Parts Rotor Lid Rotor Lid Knob Rotor Lid O-ring Rotor O-ring Tie-down Screw 3637 T-handle Rotor Wrench -26

30 SX24.5 Biosafety 24 x 2.2 ml SX24.5 Axis of Rotation rmin (35 mm) rav (54 mm) rmax (74 mm) Swinging Bucket Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 4 000* N.A. 24 x 2.2 ml 52.8 ml x 45 mm 0.44 x.8 in. For use in Allegra X-22 Series centrifuges. No SX24.5 Biosafety Rotor Assembly. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyallomer Natural, Attached cap x * Polyethylene Heparin-Lithium-Fluoride Coated 250 μl x Heparin-Lithium Coated 250 μl x Plain 250 μl x Heparin-Lithium-Fluoride Coated 400 μl x Heparin-Lithium Coated 400 μl x Plain 400 μl x Attached cap x Polypropylene Natural, Separate Cap x * 500- to 750-μL Tubes 500 μl/ μl * In refrigerated centrifuges; maximum speed in unrefrigerated centrifuges is rpm. In refrigerated centrifuges; g-force at r max in unrefrigerated centrifuges is 9 55 g. No adapter is required with this tube; however, adapter (pkg. of 2) can be used. ** Commercially available. Rotor Re placement Parts Rotor Lid Assembly O-ring Bucket (set of 2) Tie-down Screw T-handle Rotor Wrench Adapters

31 S040 4 x 0 ml S040 Axis of Rotation r min (26 mm) r av (6 mm) r max (96 mm) 90 Swinging-Bucket Rotor, Anodized Aluminum Major applications: Rapidly sediments protein precipitates, large particles, cells, cell debris, and for separations using gradients. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size x 0 ml 40 ml 6 x 8. mm 0.65 x 3.25 in For use in the Allegra 64R centrifuge. No S040 Swinging-Bucket Rotor Assembly, for rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottle with Cap Assembly Polycarbonate x Bottles with Screw Caps Polyallomer x Teflon * x Tubes Polyallomer, Thick-wall x x Polycarbonate x x Ultra-Clear x * Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. To facilitate tube removal, place rubber pad (part number 34420) at the bottom of the tube cavity before inserting the tube. Rotor Re placement Parts Buckets (set of 4) Tie-down Screw 3637 T-handle Rotor Wrench -28

32 TS Biosafety * 4 x 500 ml TS Axis of Rotation r min (87.0 mm) r av (38.5 mm) r max (90.0 mm) Swinging Bucket Rotor, Aluminum Major applications: Sedimenting protein precipitates, large particles, cells, and cell debris. Max. RPM Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) x 500 0:59/0:47 85 x 35 mm 3.5 x 5.5 in. For use in the Allegra 25R centrifuge. No TS Swinging Bucket Biosafety Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assembly Polycarbonate x Polypropylene x Bottles with Screw Caps Polyallomer x Polycarbonate x x Polypropylene x Tubes with Caps Polyallomer x Open-Top Tubes Polyallomer, Thick-wall x Polycarbonate x x * With sealed bucket covers. Rotor Re placement Parts Buckets (set of 2) Bucket Cover (set of 2) Bucket O-ring (set of 8) Microplate Carrier (set of 2) Tie-down Screw T-handle Wrench Adapters

33 SX x 250 ml SX mm 0 mm 72 mm rmin rav rmax Swinging-Bucket Rotor, Aluminum Major applications: Rapidly sediments protein precipitates, large particles, cells, and cell debris. Can also be used for binding studies and for separating serum from whole blood. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 4 500* x 250 ml 000 ml 6.8 x 25 mm 2.5 x 5 in Axis of Rotation For use in Allegra X-22 Series centrifuges. No SX4250 Swinging-Bucket Rotor. Unshielded, four-place rotor with aluminum rotor yoke and removable aluminum swinging buckets, all anodized black for corrosion protection. Includes four buckets without covers. Bottles and Tubes Tube Style Nominal Size Required Adapter Tubes per Maximum Number Volume per (mm) (set of 2) Adapter of Tubes per Tube (ml) Rotor Microfuge/PCR Tubes 0.5 ml 7 x 9 mm Hemolyse or RIA Tubes.5/2.2 ml x 38 mm ml 2 x 75 mm ml 2 x 75 mm Vacutainer 5 ml 3 x 75 mm Glass 7 ml 2 x 00 mm Vacutainer 0 ml 6 x 00 mm Monovettes 0 ml 7 mm Round-Bottom 0 ml 6. x 8. mm /5 ml 7 x 0 mm Conical 5 ml 7 x 20 mm Glass with Open-Top or Screw-on Cap 25 ml 24 x 00 mm Glass 25 ml 24 x 00 mm Round-Bottom 50 ml 28.5 x 07 mm Conical 50 ml 28.5 x 00 mm Glass 50 ml 34 x 00 mm /00 ml 44 x 00 mm ml Oakridge 94 ml 38 x 2 mm Flat-bottom with Screw-on Cap 25 ml 50.5 x 99 mm ml 56.5 x 3 mm ml 6.8 x 25 mm * In refrigerated centrifuges; maximum speed in unrefrigerated centrifuges is rpm. In refrigerated centrifuges; g-force at r max in unrefrigerated centrifuges is x g. Replacement Parts Bucket Covers (set of 4) Bucket with Cover (set of 2) Bucket O-ring (set of 4) Tie-down Screw T-handle Rotor Wrench Adapters

34 SX4750/4750A Biosafety * 4 x 750 ml SX4750/4750A r min r av r max (207.8 mm) Axis of Rotation Swinging-Bucket Rotor, Stainless Steel/Aluminum Major applications: Rapid sedimentation of protein precipitates, large particles, cells, and cell debris. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x 750 ml 0:80/0:80 96 x 30 mm 3.85 x 5.25 in r min r av r max (83.2 mm) Axis of Rotation For use in Allegra X-5R and Allegra X-2 Series centrifuges. No SX4750 Swinging-Bucket Biosafety Rotor. Four-place rotor with stainless-steel rotor yoke and removable aluminum swinging buckets. Buckets are interchangeable with SX4750μ Biosafety Microplate Carriers for spinning microtiter plates or microfuge tubes using rack inserts. Bucket Covers No Set of two. Transparent bucket covers for SX4750 round rotor buckets to contain broken tubes. Independently certified by CAMR, Porton Down, UK, for biocontainment. Aerosolve Cannisters No Set of four. No Set of two. Aerosolve Cannisters fit in SX4750 round rotor buckets. These Biosafety cannisters feature an O-ring seal and are completely transparent so a broken tube can be seen and proper precautions taken before you break the seal. Cannisters can also be used as 500-mL wide-mouth bottles. Specially designed adapters accommodate most popular tubes within the cannister. Independently certified by CAMR, Porton Down, UK, for biocontainment. In the Tubes and Bottles chart following, adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Cell Culture Flask Adapters No Accommodate Corning 75 cm 2 Canted-Neck Cell Culture Flasks (PN 43064). One flask per adapter rpm; x g. No Accommodate Corning 25 cm 2 Canted-Neck Cell Culture Flasks (PN ). Two flasks per adapter rpm; x g. No SX4750A Swinging-Bucket Biosafety Rotor. Four-place rotor with stainless-steel rotor yoke and removable aluminum swinging buckets. ARIES technology provides imbalance correction for rotors with buckets that are unbalanced up to 50 grams in opposing loads. SX4750μ Biosafety Microplate Carriers No SX4750μ Biosafety Microplate Carrier Assembly. Includes carriage and rubber pad to cushion plates. Each carrier can hold up to 3 microplates, deep-well/square well plate, rack of MiniTubes, or other labware in 96-well format. Set of 2. Maximum allowable speed in Allegra X-2 is rpm (2 890 x g), in Allegra X-5R, maximum allowable speed is rpm (4 060 x g). Replacement Parts for SX4750/SX4750A Bucket (set of 2) Bucket covers (set of 2) Replacement bucket O-ring (pkg/8) Bucket cover latch assembly Replacement Parts for SX4750μ Microplate Carriers Bio-certified covers for SX4750μ microplate carriers Replacement cover gasket (set of 4) Replacement carriage, includes rubber pad (set of 2) Replacement rubber pad for carriage (set of 4) * With sealed bucket covers. Rated at rpm/3 270 x g in the Allegra X-2 Series centrifuges. Patent pending. Corning is a trademark of Corning Incorporated. -3

35 SX4750/4750A (cont d.) Biosafety * 4 x 750 ml SX4750/4750A Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Modular Tubes per g-force* k Factor Maximum Volume per (mm) Disk Adapters Adapter Speed* Tube (ml) Set of 2/Set of 4 Bottles, Wide-mouth Polycarbonate x Polypropylene x Polypropylene x Bottles with Cap Assemblies Polyallomer x / / x / / Polycarbonate x / / Polycarbonate, Wide-mouth x Polypropylene, Wide-mouth x x Bottles with Screw Caps Polyallomer x / /35964 Polycarbonate x / / x x Polypropylene x Polypropylene, Wide-mouth x Teflon with High-Speed Cap x / / * Rated at RPM/3 270 x g Allegra X-2 Series centrifuges Adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type.. Adapters

36 SX4750/4750A (cont d.) Biosafety * 4 x 750 ml SX4750/4750A Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Modular Tubes per g-force* k Factor Maximum Volume per (mm) Disk Adapters Adapter Speed* Tube (ml) Set of 2/Set of 4 Open-Top Tubes Polyallomer, Thick-wall x / / Polyethylene x / Polycarbonate x / / x / x / /35964 Polycarbonate, Graduated x / /35964 Polypropylene x / x / /35964 Stainless Steel x / / Tubes with Snap-On Caps Polyallomer x / / 24 Polycarbonate x / /35964 Polycarbonate, Conical x / ** / Polyethylene x / / 24 Polypropylene, Conical x / ** / * Rated at rpm/3 270 x g in the Allegra X-2 Series centrifuges. Adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Requires.5-mL adapter plate PN ** Requires adapter PN , available each pack. Adapters

37 SX4750/4750A (cont d.) Biosafety * 4 x 750 ml SX4750/4750A Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Modular Tubes per g-force* k Factor Maximum Volume per (mm) Disk Adapters Adapter Speed* Tube (ml) Set of 2/Set of 4 Tubes with Snap-On Caps (continued) Polypropylene x / /35964 Orange x / / Yellow x / / Green x / / Blue x / / Natural x / / Natural** x / / BioVials Polypropylene x / / Blood-Bag Cups No Yellow cup with inner diameter of 90 mm for singleor double-packs. No Orange cup with inner diameter of 97 mm for tripleor quad-packs. SX4750μ Biosafety Microplate Carriers No SX4750μ Biosafety Microplate Carrier Assembly. Includes carriage and rubber pad to cushion plates. Each carrier can hold up to 3 microplates, deep-well/square well plate, rack of MiniTubes, or other labware in 96-well format. Set of 2. Maximum allowable speed is rpm (2 890 x g). * Rated at rpm/3 270 x g in the Allegra X-2 Series centrifuges, rpm/4 060 x g in Allegra X-5R. Adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Requires.5-mL adapter plate PN 3545, sold individually. ** Separate cap. Rotor Supplies Round Bucket (set of 2) Round Bucket Cover (set of 2) Round Bucket O-ring (pkg of 8) Round Bucket Cover Latch Assembly Bucket Cover Air-Vent Filter (pkg of 60) Adapters

38 S x Microplates S5700 Axis of Rotation r min 38.6 mm r av 53.4 mm r max 68.5 mm Swinging-Bucket Rotor, Aluminum Major applications: DNA sample preparation in microplates, deep-well plates, 96-well filtration kits, and PCR plates. Max. RPM Max. g Number of Plates Approximate Volume/Size Accel/Decel Time (min:sec) x 96-well plates 0:47/0:20 per side For use in the Allegra 25R centrifuge. No S5700 Swinging Bucket Rotor. Microplates No. of Wells Description Part No. Quantity g-force Maximum Speed 96 Multiwell Polystyrene plate pkg Deep-well (-ml/well) Polystyrene Titer Plates, nonsterile /ctn Deep-well (-ml/well) Polystyrene Titer Plates, sterile /ctn Deep-well (-ml/well) Polypropylene Titer Plates, nonsterile /ctn Deep-well (-ml/well) Polypropylene Titer Plates, sterile /ctn Square-well Polypropylene Titer Plates /ctn Rotor Re placement Parts S5700 Buckets (set of 2) S5700 Microplate Carrier (set of 2) Tie-down Screw T-handle Rotor Wrench Support Pad for Microplates (set of 4) Rubber Roller, 4 in., for Sealing Foil Microplate Lids -35

39 S x Microtiter Plates S2096 Axis of Rotation rmin (70 mm) rav rmax (0 mm) Swinging-Bucket Rotor (Unshielded), Anodized Aluminum Major applications: Serial dilution of small liquid volumes. Max. RPM Max. g Number of Plates Approximate Volume/Size Accel/Decel Time (min:sec) x 96 wells 0:2/0:20 For use in Allegra X-22 Series and discontinued Allegra 2 Series centrifuges. No. 36. S2096 Microtiter Rotor Assembly, for rpm operation. Unshielded, two-place rotor, designed to accommo date 96-well and deepwell microplates. Racks are available that accommodate -ml MiniTubes. Microplates No. of Wells Description Part No. Quantity g-force Maximum Speed 96 Microplates pkg Deep-well (-ml/well) Polystyrene Titer Plates, nonsterile /ctn Deep-well (-ml/well) Polystyrene Titer Plates, sterile /ctn Deep-well (-ml/well) Polypropylene Titer Plates, nonsterile /ctn Deep-well (-ml/well) Polypropylene Titer Plates, sterile /ctn Square-well Polypropylene Titer Plates /ctn Accessories for Microplates No Caps for 96-well Deep-well Titer Plates, Nonsterile (0/ctn). No Caps for 96-well Titer Plates, Sterile (0/ctn). MiniTube Racks, Tubes, and Accessories No MiniTube Rack No MiniTube Test Tubes (-ml), Polystyrene, Nonsterile (400 strips of 2) No MiniTube Test Tubes (-ml), Polypropylene, Nonsterile (400 strips of 2) No Caps for MiniTubes, Polyethylene, Nonsterile (80 strips of 2) No Caps for MiniTubes, Polyethylene, Sterile (80 strips of 2) Rotor Re placement Parts Tie-down Screw 3637 T-handle Rotor Wrench 362 Tray (set of 2) -36

40 Microcentrifugation Microfuge 6 Centrifuge Microfuge 8 Centrifuge Microfuge 22R Centrifuge Microfuge Centrifuges Rotor Summary Fixed-Angle Rotors F F24.5P F F F2x FX2.5P...2- FX24.5P Swinging Bucket Rotor S

41 Microcentrifugation Microfuge 6 Microcentrifuge The Microfuge 6 is fast and powerful for optimal pelleting on the bench or in the cold room. Perfect for DNA, RNA, proteins, and virus cell isolation. Its maintenance-free motor spins up to rpm (6 63 x g). Easy-to-read interface displays both speed (rpm) and force (rcf). Comes complete with a 24-place, aerosol-tight, autoclavable, fixed angle rotor. A convenient snap-on rotor lid (not aerosol tight) is included as an accessory to the rotor. Select timed, hold, or pulse (short) run times. 2 Specifications Maximum Speed Maximum g-force Maximum capacity Drive Type Time Setting Accel/Decel Rates* Power Consumption Maximum Noise Output Dimensions Weight rpm 6 63 x g 24 x 2.2 ml Brushless 0 99 minutes, 59 seconds, continuous, pulse (short run) 5 sec accel/3 sec decel to/from max speed 95 W 60 dba 7.6 cm (6.9 in) H x 26.6 cm (0.5 in) D x 22.6 cm (8.9 in) W 6.4 kg (4 lb) Part Numbers A46472 A4647 A46474 A46473 Microfuge 6 Microcentrifuge 50/60 Hz, 00/20 V, with FX24.5P rotor 50/60 Hz, 220/240 V, with FX24.5P rotor 50/60 Hz, 00/20 V 50/60 Hz, 220/240 V * while using the FX24.5P rotor. 2-2

42 Microcentrifugation Microfuge 8 Microcentrifuge The Microfuge 8 is fast and powerful for optimal pelleting on the bench or in the cold room. Perfect for DNA, RNA, proteins, and virus cell isolation. Its maintenance-free motor spins up to rpm (8 000 x g). Easy-to-read interface displays both speed (rpm) and force (rcf). Comes complete with a 24-place, aerosol-tight, autoclavable, fixed angle rotor. A convenient snap-on rotor lid is available as an accessory to the rotor. Select timed, hold, or pulse (short) run times. 2 Specifications Maximum Speed rpm Maximum g-force x g Drive Type Brushless Time Setting 0 30 minutes, continuous, pulse (short run) Accel/Decel Rates 8 sec accel/9 sec decel to/from max speed Maximum Heat Output 70 Btu/h (50 W) Maximum Noise Output 58 dba Dimensions 24.0 cm (9.5 in) H x 32.0 cm (2.6 in) D x 24.5 cm (9.7 in) W Weight 3.0 kg (28.7 lb) Microfuge 8 Microcentrifuge Part Numbers /60 Hz, 00/20 V, with F24.5P rotor Hz, 230 V, with F24.5P rotor 2-3

43 Microcentrifugation Microfuge 22R Microcentrifuge The Microfuge 22R refrigerated microcentrifuge offers high g -force (2 920 x g) for optimal pelleting. its maintenance-free motor spins up to rpm and maintains 4 C at maximum speed to protect precious samples. Perfect for DNA, RNA, proteins, and virus cell isolation. Available with six interchangeable rotors, all of which feature aerosol-tight lids to minimize contamination from broken or leaking tubes. Convenient snap-on rotor lids are available as accessories to the F24.5, F24.5P, and F40.25 rotors. All rotors are also autoclavable at 2 C. Easy-to-use control knobs and easy-toread display. 2 Specifications Maximum Speed rpm Maximum g-force x g Drive Type Brushless induction Time Setting 0 30 minutes, continuous, pulse (short run) Accel/Decel Rates 8 sec accel/9 sec decel to/from max speed Maximum Heat Output 2 50 Btu/h (630 W) Maximum Noise Output 58 dba Dimensions 36.5 cm (4.4 in) H x 58.5 cm (23.0 in) D x 30.0 cm (.8 in) W Weight 4.0 kg (90.4 lb) Microfuge 22R Part Numbers Hz, 20 V Hz, 230 V /60 Hz, 00 V Hz, 20 V with F24.5P rotor Hz, 230 V with F24.5P rotor /60 Hz, 00 V with F24.5P rotor A Hz, 20 V with F30.5 rotor A Hz, 230 V with F30.5 rotor A /60 Hz, 00 V with F30.5 rotor 2-4

44 Microcentrifugation Microfuge Centrifuges Rotor Summary Rotor Part Maximum Maximum Number Tubes/Bottles Rotor Approx. Rotor Biosafe Type Number Speed Force (g) and Size Capacity Accel. Angle (rpm) at r max (diameter x length) (ml) Time* mm / in. (min:sec) Fixed-Angle Rotors Microfuge 6 Microfuge 8 Microfuge 22R F x :9 45 Yes x x.5 Pellet subcellular organelles, DNA, viruses, bacteria, or chloroplasts. Biosafety 2 F24.5P x :8 45 No x x.5 Pellet subcellular organelles, nucleic acids, viruses, bacteria, or chloroplasts. F x :40 45 Yes x 39 Biosafety 0.44 x.8 Pellet subcellular organelles, viruses, bacteria, chloroplasts, mitochondria, or algae. F x 0.25/ :9 30 Yes 7 x 40 Biosafety 0.25 x.5 Pellet subcellular organelles, DNA, viruses, bacteria, or chloroplasts. F2x / x 8 (200 μl) 9.2 0:38 45 No /8 820 PCR strip Minimize post-pcr condensation, post-reaction cleanup. FX2.5P A x.5/ :3 45 No x x.6 Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts, or algae. FX24.5P A x.5/ :5 32 /37 No x x.6 Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts, or algae. Swinging Bucket Rotor S x :30 90 No x x.8 Pellet subcellular organelles, viruses, bacteria, chloroplasts, mitochondria, or algae. * To maximum speed, rotor fully loaded. Tubes and 8: outer/inner. Tubes 4 and 5: outer/inner. 2-5

45 F x 2.2 ml F24.5 Biosafety * Axis of Rotation 45 r min (50 mm) rav (65 mm) r max (80 mm) Fixed Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, DNA, viruses, bacteria, or chloroplasts. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x.8 ml 0:9/0:23 x 39 mm 0.44 x.5 in For use in the Microfuge 22R microcentrifuge. No F24.5 Biosafety Rotor Assembly. Transparent high-impact plastic lid. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapter Adapter Speed Tube (ml) Polyallomer Natural, Attached cap x Polyethylene Heparin-Lithium-Fluoride Coated 250 μl x Heparin-Lithium Coated 250 μl x Plain 250 μl x Heparin-Lithium-Fluoride Coated 400 μl x Heparin-Lithium Coated 400 μl x Plain 400 μl x Attached cap x Polypropylene Natural, Separate Cap 400 μl x x * With high-impact lid. Rotor Re placement Parts Convenient Snap-on Lid Rotor Lid Assembly Rotor Lid Knob O-ring Tie-down Screw T-handle Rotor Wrench Adapters

46 F24.5P 24 x.8 ml F24.5P 45 r min (50.2 mm) r av (67.4 mm) r max (82.5 mm) Axis of Rotation Fixed Angle Rotor, High-Impact Thermoplastic Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, or chloroplasts. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x.8 ml 0:8/0:9 x 39 mm 0.44 x.5 in For use in Microfuge 6, 8, and 22R microcentrifuges. No F24.5P Rotor Assembly. Rotor is made of high-impact thermoplastic and snap-on lid is polycarbonate. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyallomer Natural x Polyethylene 250 μl x μl x μl x μl x μl x μl x x Polypropylene Natural 400 μl x x Rotor Re placement Parts Convenient Snap-on Lid Aeroseal-tight Lid Tie-down Screw T-handle Rotor Wrench Tie-down Screw for Snap-on Cap Rubber Seal, Lid Adapters

47 F30.5 Biosafety 30 x 2.2 ml F r min (67 mm) r av (84 mm) r max (00 mm) Axis of Rotation Fixed Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x 2.2 ml 0:40/0:38 x 45 mm 0.44 x.8 in. For use in the Microfuge 22R microcentrifuge. No F30.5 Biosafety Rotor Assembly. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapter Adapter Speed Tube (ml) Polyallomer Natural, Attached cap x * Polyethylene Heparin-Lithium-Fluoride Coated 250 μl x Heparin-Lithium Coated 250 μl x Plain 250 μl x Heparin-Lithium-Fluoride Coated 400 μl x Heparin-Lithium Coated 400 μl x Plain 400 μl x Attached cap x * Polypropylene Natural, Separate Cap x * to 750-μL Tubes 500 μl/ μl 2 * No adapter is required with this tube; however, adapter (pkg. of 2) can be used. Commercially available. Rotor Re placement Parts Rotor Lid Assembly Lid O-ring Rotor O-ring Tie-down Screw T-handle Rotor Wrench Adapters

48 F40.25 Biosafety 40 x 0.25/0.40 ml F r min (55.7 mm) rav (68.4 mm) rmax (80.0 mm) Axis of Rotation Fixed Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x 0.40 ml 0:40/0:38 7 x 40 mm 0.25 x.5 in. For use in the Microfuge 22R microcentrifuge. No F40.25 Biosafety Rotor Assembly. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyethylene 250 μl x μl x μl x μl x μl x μl x Rotor Re placement Parts Convenient Snap-on Lid Rotor Lid Assembly Lid O-ring Rotor O-ring Tie-down Screw T-handle Rotor Wrench Adapters

49 F2x8.2 2 x 8 Strips of 200 μl tubes F2x rmax (inner row) rmax (outer row) Axis of Rotation Fixed Angle Rotor, Aluminum Major applications: Minimizing post-pcr * condensation, post-reaction clean-up. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) (outer row) 2 x 8 Strips 0:38/0: (inner row) 200 μl For use in the Microfuge 22R microcentrifuge. No F2x8.2 Rotor Assembly. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) PCR Tubes 200 μl * PCR is covered by patents owned by F. Hoffman-La Roche, Inc. Commercially available. Rotor Re placement Parts Rotor Lid Assembly Rotor O-ring Lid O-ring Tie-down Screw T-handle Rotor Wrench 2-0

50 FX2.5P 2 x.5/2.2 ml FX2.5P r max (62 mm) r av (45.5 mm) 45 r min (29 mm) Fixed Angle Rotor, High-Impact Thermoplastic Major applications: Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts, or algae. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x.5/2.2 ml 0:3/0: x 40 mm 0.44 x.6 in For use in Microfuge 6 No. A FX2.5P Rotor Assembly. Rotor is made of high-impact thermoplastic and snap-on lid is polysulfone. Tubes Axis of Rotation Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyallomer Natural x Polyethylene 250 μl x μl x μl x μl x μl x μl x x Polypropylene x PCR Tubes 200 μl /600/750 μl * PCR is covered by patents owned by F. Hoffman-La Roche, Inc. Commercially available. Rotor Re placement Parts A46477 Convenient Polysulfone Snap-on Lid Adapters

51 FX24.5P 24 x.5/2.2 ml FX24.5P Inner Row Dimensions r max (62 mm) r av (45.5 mm) 37 (outer row) r min (38 mm) 32 (inner row) r min (38 mm) r av (57 mm) r max (66 mm) Axis of Rotation Fixed Angle Rotor, High-Impact Thermoplastic Major applications: Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts, or algae. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x.5/2.2 ml 0:5/0:3 x 40 mm 0.44 x.6 in For use in Microfuge 6 No. A FX24.5P Rotor Assembly. Rotor is made of high-impact thermoplastic and snap-on lid is polysulfone. Tubes Outer Row Dimensions Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyallomer Natural x Polyethylene 250 μl x μl x μl x μl x μl x μl x x Polypropylene x PCR Tubes 200 μl /600/750 μl * PCR is covered by patents owned by F. Hoffman-La Roche, Inc. Commercially available. Rotor Re placement Parts A46477 Convenient Polysulfone Snap-on Lid Adapters

52 S x 2.2 ml S24.5 Axis of Rotation rmin (35 mm) rav (54 mm) rmax (74 mm) Swinging Bucket Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. RPM Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) x 2.2 ml 0:30/0:33 x 45 mm 0.44 x.8 in. For use in the Microfuge 22R microcentrifuge. No S24.5 Rotor Assembly. Tubes and Bottles Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyallomer Natural x Polyethylene 250 μl x μl x μl x μl x μl x μl x x Polypropylene Natural x to 750-μL Tubes 500 μl/ μl 2 Rotor Re placement Parts Rotor Lid Assembly O-ring Bucket (set of 2) Tie-down Screw T-handle Rotor Wrench Adapters

53 Tubes and Bottles Tubes and Bottles for Every Application No single tube design or material will meet all application requirements. A number of factors should be considered when a supply of tubes is ordered: the par ticu lar technique to be used, the nature of the sample and any solvent or gradient media, the desirability of reusing the tubes, and certain convenience factors. The properties listed below provide a guide for anyone involved in the tube se lection process. 3 Strength and Flexibility, to resist permanent deformation even when run in fixed angle rotors without tube caps Chemical Resistance to a wide range of bases, acids, and solvents Transparency, to permit a clear view of fractions and bands after centrifugation Thin enough to be sliced or punctured after centrifugation for fraction collection Impermeable to Water, to prevent aqueous solutions from permeating the tube wall and reaching the rotor cavity Surface Properties that prevent the adherence of nucleic acids and proteins Temperature Tolerance throughout a wide range of operating temperatures, without deforming at high temperatures or cracking when used close to 0 C Autoclavable, for convenient sterilization and reuse Contaminant-free, to avoid leaching extraneous materials into the sample, especially materials visible in the sensitive nm range Odor-free, for pleasant handling The full line of Beckman Coulter tubes includes a number of tube materials, each with its own distinct combination of prop erties to meet a variety of application requirements. Available are transparent, translucent, and opaque tubes; tubes that can be sliced or punctured; tubes that can be sterilized and reused; and tubes that are resistant to a variety of chemical com pounds. 3-

54 Tubes and Bottles Tube Selection Considerations Compatibility of Tube Material with Solvents and Sample The chemical compatibility of the tube materials with the gradient-forming medium or other solvent is a prime con sider ation. Neutral sucrose and salt solutions cause no problem. But alkaline solutions, such as those frequently used for the separation of single-stranded forms of DNA, cannot be used in Ultra-Clear tubes or polycarbonate tubes and bottles. Sometimes DMSO is used in preparation of sucrose gradients for sedimentation of denatured RNA. Poly car bonate and Ultra- Clear tubes are incompatible with DMSO, so polyallomer tubes should be used. The last column of the Quick Reference Chart to Tube Materials and Their Properties on page 3-4 gives some guidelines to the chemical resistances of the various tube materials. It must be em pha sized, however, that other conditions of cen trifu gation (g-force, duration of run, etc.) have con sid erable effect on how well a tube material will withstand a particular solvent. Beckman Coulter pub lication IN-75, Chemical Resistances for Beckman Coulter Cen trifugation Products (found on the Beckman Coulter web site at chemres.asp) provides more detailed information about the chemical re sis tances of the various tube ma te rials. The wisest course is to test any ques tionable com bi nation under operating con ditions before making the actual run. The type of sample, in some cases, will affect selection of a specific tube material. DNA, in its denatured or singlestranded form, will adhere to the surface of some tube ma terials. Polyallomer would be the best choice. (Most of this work is done in highly alkaline media which are in com patible with polycarbonate.) Lipoprotein separations are most often done in Ultra-Clear tubes because they are clear and sliceable; these properties simplify fraction location and recovery by tube slicing. When small lipoprotein samples are to be recovered by a frac tionating device and clear tubes are desirable, there are alternatives: cellulose propionate, polycarbonate, and Ultra-Clear tubes. Hazardous materials, either pathogenic or radioactive, should be centrifuged with extreme care. All possible precautions must be taken to avoid leakage of the sample into the rotor cavity during centrifugation. To determine the optimum tube material for your specific sample and gradient medium, refer to the quick reference chart on page 3-4. Gradient Formation and Fractionation When choosing a tube for a density gradient run, some thought should be given to gradient formation and frac tion ation. If the bands or zones formed during centrifugation are indistinct, they may not be visible through a translucent material such as polyallomer. If optimum band visualization is important, Ultra- Clear tubes or tubes of polycarbonate or cellulose propionate should be used. Whenever collection of bands or zones must be done by puncturing the tube or slicing, a thin, flexible tube wall is required. Ultra-Clear or polyallomer tubes should be used, depending on the need for transparency. As there are currently no wettable plastic centrifuge tubes available, gradients should be loaded into plastic tubes from the bottom up to avoid mixing. High Temperature Centrifugation Although modern centrifuges and rotors can operate at tem peratures as high as 45 C, one cannot assume that every tube can be safely run over 25 C. Stainless steel and glass are the only materials which will not experience some de for mation when subjected to high temperatures and long cen trifugation times. Plastic tubes undergo some degree of softening at temperatures higher than 25 C. Whether or not this will cause permanent deformation is not a question of temperature alone. The centrifugal force field used, the duration of the cen trifu gation, the type of rotor, and even the tube angle all have an effect. It s obviously impossible to give exact temperature limits for plastic tubes when so many other variables are involved. The safest policy is to pretest the tubes under the actual ex peri men tal conditions, but with water, rather than a valuable sample. Tube Sizes Tube sizes as indicated in the following charts are nominal sizes, and may vary somewhat from actual filling capacities. If a thickwall tube is run uncapped, the maximum filling volume will depend on the tube angle of the rotor to be used. See appropriate rotor instruction manuals for maximum filling levels of tubes. Tube Cleaning, Sterilization, and Reuse If tubes are to be reused, special care must be taken during cleaning and sterilization. All tubes can be washed by hand with a mild detergent such as Solution 555 diluted 5-to- or 0-to- with water. This is particularly important for poly carbonate tubes and bottles which should not be exposed to a detergent with a ph higher than 8. Tubes and bottles should not be washed in commercial dishwashers as the detergents and high tem pera tures are too harsh. Solvents such as alcohol or acetone react unfavorably with many tube materials. If an organic solvent must be used in the cleaning procedure, consult bulletin IN-75 for a table of tube material/solvent compatibilities (or review the same document on the Beckman Coulter web site at labresources/centrifuges/chemres.asp). The method chosen for sterilization has direct bearing on the number of reuses one can expect from a tube. Tubes and bottles of polyallomer, polyethylene, and glass can all be autoclaved, although in general, cold sterilization methods are not as harsh as autoclaving. Cold sterilization is rec ommended for both polycarbonate and Ultra-Clear

55 Tubes and Bottles If maximum reuse is a major consideration, either polyallomer (preferably thickwall) or polycarbonate tubes and bottles should be selected, and cold sterilization methods used. If these tubes are run completely filled in swinging bucket rotors, most of them can be reused a number of times. Chances of permanent de for mation will be greater whenever the tubes are run in fixed angle rotors, without caps, and/or partially filled. All of these con ditions tend to stress the centripetal edge of the tube unduly. All tubes that have been used or autoclaved previously must be individually examined for signs of deformation or cracking before using them again. Tube Closures When other considerations have been resolved, convenience may be a deciding factor. Without a doubt, the most convenient tube closure is none at all; none are required for tubes run in swinging bucket rotors. For tubes run in fixed angle rotors, alternatives to the standard tube cap assemblies are available. Bottles have three-piece cap assemblies which are easier to use than the more complex tube cap assemblies. Polycarbonate bottles are available for general-purpose fixed angle rotors, and are used frequently for differential cen trifu gation where band re cov ery is not a problem. Thickwall tubes can be run in all fixed angle rotors without caps, provided they are partially filled. (Refer to rotor manuals for more information on fill volumes.) When closed tubes are required, Beckman Coulter offers some innovative and con ve nient options

56 Tubes and Bottles A Quick-Reference Chart to Tube Materials and Their Properties Property Thinwall Thickwall Ultra-Clear Polycarbonate Polypropylene Polyethylene Cellulose Polyallomer Polyallomer Propionate Optical transparent translucent transparent transparent transparent transparent/translucent transparent Autoclaveable yes yes no no yes no no Puncturable yes no yes no no yes no Sliceable yes no* yes no* no no no* Reusable no yes no yes yes yes no Acids (dilute S S S S S S S or weak) Acids (strong) U S U U S S U Alcohols (aliphatic) U S U U S S U Aldehydes M M S M M S U Bases S S U U S S U Esters U M U U M S M Hydrocarbons U M U U S U S (alphatic) Hydrocarbons U U U U M M S (aromatic and halogenated) Ketones U M U U M M U Oxidizing U U U M M M M Agents (strong) Salts S S M M S S S S = satisfactory resistance M = marginal resistance U = unsatisfactory resistance * Polyallomer, polycarbonate, and cellulose propionate tubes with diameters of 5 to 3 mm may be sliced using the CentriTube Slicer (part number ) and appropriate adapter plate. Note: This information has been consolidated from a number of sources and is provided only as a guide to the selection of tube materials. Soak tests at g (at 20 C) established the data for most of the materials; reactions may vary under the stress of centrifugation, or with extended contact or temperature variations. To prevent failure and loss of valuable sample, ALWAYS TEST SOLUTIONS UNDER OPERATING CONDITIONS BEFORE USE. 3 Warning: Do not use fl ammable substances in or near an operating centrifuge. 3-4

57 Tubes and Bottles General Filling and Sealing Requirements for Tubes and Bottles Tube or Bottle Swinging-Bucket Rotors Fixed-Angle Rotors Polyallomer Thinwall tubes Within 2 to 3 mm of top Full with cap Thickwall tubes At least /2 full /2 full to max. capless level or full with cap Quick-Seal tubes Full and heat-sealed Full and heat-sealed Bottles Min. to max. (see rotor manual) /2 full to max. (see rotor manual) with screw-on cap with screw-on cap or cap assembly or cap assembly Ultra-Clear Open-top tubes Within 2 to 3 mm of top Full with cap Quick-Seal tubes Not used Full and heat-sealed Polycarbonate Thickwall tubes At least /2 full /2 full capless level or full with cap or cap assembly Bottles At least /2 full Min. to max. (see rotor manual) with screw-on cap or cap assembly Stainless Steel Tubes Any level Any level with cap or cap assembly Polypropylene Tubes and bottles At least /2 full /2 to max. capless level or full with cap or cap assembly Polyethylene Tubes At least /2 full /2 to max. capless level or full with cap or cap assembly Teflon Tubes and bottles At least /2 full /2 full to max. capless level or full with cap 3 3-5

58 Tubes and Bottles Bottles Used in Benchtop Centrifuges Nominal Size Material Bottle & Bottle with Bottle Insert O-ring Screw Cap Capacity mm Cap Assy Screw Cap Only Only Only 0 ml 6 x 80 PC N.A N.A. N.A. N.A. N.A. 0 ml 6. x 8. PA N.A N.A. N.A. N.A. N.A. 0 ml 6. x 8. T N.A N.A. N.A. N.A. N.A ml 25 x 89 PC N.A ml 25.3 x 92 PA N.A. N.A. N.A. N.A. N.A. 30 ml 25.3 x 92 PC N.A N.A. N.A. N.A. N.A. 40 ml 29 x 04 PC N.A N.A. N.A. N.A. N.A. 50 ml 28.5 x 07 T* N.A N.A. N.A. N.A. N.A. 50 ml 29 x 04 PA N.A ml 29 x 04 PC N.A N.A ml 38 x 02 PC N.A ml 38 x 04 PC N.A. N.A. N.A. N.A. 85 ml 38 x 04 PP N.A N.A. N.A. N.A. N.A N.A. N.A. N.A. N.A. 00 ml 38 x 02 PP N.A N.A ml 62 x 20 W PC N.A N.A ml 62 x 22 W PP 3560 N.A N.A ml round-bottom 62 x 36 PC N.A N.A. N.A. N.A ml 69 x 60 PC N.A N.A. N.A. N.A. 500 ml 69 x 60 PP N.A ml 69 x 60 W PP N.A N.A. N.A. N.A. N.A. 500 ml 85 x 35 PC N.A N.A. N.A. N.A. N.A. 500 ml 85 x 35 PP N.A N.A. N.A. N.A. N.A. 750 ml 96 x 30 PC N.A N.A. N.A ml 96 x 30 PP N.A N.A. N.A PA = Polyallomer PC = Polycarbonate PE = Polyethylene PP = Polypropylene T = Tefl on C = Conical W = Wide-Mouth N.A. = Not Available * With high-speed screw cap. With special cap. 3-6

59 Tubes and Bottles Adapters for Non-Beckman Coulter Tubes/Bottles* Rotor Tube Vol. Size (mm) # of Places Part Number SX ml 7 x 9 mm ml 2 x 75 mm ml Hemolyse/RIA ml 3 x 75 mm ml 2 x 00 mm ml 6 x 00 mm ml 7 mm ml 6. x 8. mm /5 ml 7 x 0 mm ml 6 x 25 mm ml 7 x 20 mm ml 24 x 00 mm ml 28.5 x 00 mm ml 28.5 x 07 mm ml 28.5 x 00 mm ml 34 x 00 mm /94 ml 38 x 2 mm /00 ml 44 x 00 mm ml 50.5 x 99 mm ml 56.5 x 99 mm ml 6.8 x 25 mm FX600 Conical 5 ml 7 x 00 mm ml ml Conical 50 ml 28.5 x 20 mm FX μl H μl SX μl S μl F μl Adapters for Glass Tubes in Beckman Coulter Rotors* Rotor Tube Volume Tube Material Adapter Part Number SX /00 ml Glass ml Glass ml Glass with Open-top or Screw-on Cap ml Glass * Check with tube manufacturer for maximum allowable g-force. Corex and Pyrex are registered trademarks of Corning Glass Works, Inc. 3-7

60 Tubes and Bottles Tubes and Bottles Used in Benchtop Centrifuges and Microcentrifuges Nominal Nominal Inches Part Rotors Filling Size No. Capacity (ml) (mm) Open-Top Tubes, Polyallomer, Thickwall* x x F00, S x x F00, SX4750, SX4750A, S040, TS , FX x 89.0 x F x 02.5 x F0485, F0685, FX600 Open-Top Tubes, Polycarbonate, Thickwall* x x F00, S040, TS x x F00, SX4750, SX4750A, S040, TS , FX x x SX4750, SX4750A, FX x x F0485, F0650, F0850, F0685, SX4750, SX4750A, TA-4-50, TA-0-250, FX x x (conical, grad.) F0650, F0850, SX4750, SX4750A, FX600, TA x 02.5 x F0485, F0685, FX600 Open-Top Tubes, Polyallomer x 76.0 x F x 02.5 x F0485, F0685 Open-Top Tubes, Polyethylene, Plain x x F202, F2402H, F24.5, F24.5P, F30.5, F3602, F40.25, FX30.5, H6002, S24.5, SX24.5, TA x x F202, F2402H, F24.5, F24.5P, F30.5, F3602, F40.25, FX30.5, H6002, S24.5, SX24.5, TA x x SX4750, SX4750A, FX600 Open-Top Tubes, Polyethylene, Heparin/Lithium Coated x x F202, F2402H, F24.5, F24.5P, F30.5, F3602, F40.25, FX30.5, H6002, S24.5, SX24.5, TA x x F202, F2402H, F24.5, F24.5P, F30.5, F3602, F40.25, FX30.5, H6002, S24.5, SX24.5, TA-5-.5 Open-Top Tubes, Polyethylene, Heparin/Lithium/Fluoride Coated x x F202, F2402H, F24.5, F24.5P, F30.5, F3602, F40.25, FX30.5, H6002, S24.5, SX24.5, TA x x F202, F2402H, F24.5, F24.5P, F30.5, F3602, F40.25, FX30.5, H6002, S24.5, SX24.5, TA-5-.5 Open-Top Tubes, Polypropylene x x SX4750, SX4750A, FX x x F0485, F0650, F0850, F0685, SX4750, SX4750A, TA-4-50, TA-0-250, FX x x SX4750, SX4750A Open Top Tubes, UltraClear x x F00, S x 89.0 x F0630 Tubes with Snap-On Attached Caps, Polyallomer.5 x x Natural F202, F2402H, F24.5, F24.5P, F30.5, F3602, FX30.5, SX4750, H6002, PTS-2000, S24.5, SX24.5, TA-5-.5, TS , FX600 3 * Nominal fi ll volume for these tubes can vary from 6.0 to 0.0 ml, depending on the rotor in which it is used due to different tube angles. 3-8

61 Tubes and Bottles Nominal Nominal Inches Part Rotors Filling Size No. Capacity (ml) (mm) Tubes with Snap-On Attached Caps, Polycarbonate* x x F0850, SX4750, SX4750A, TA-4-50, TA Tubes with Snap-On Attached Caps, Polyethylene.8 x x Natural F202, F2402H, F24.5, F24.5P, F3602, SX4750, SX4750A, H6002, S24.5, SX24.5, TA-5-.5, FX600 Tubes with Snap-On Attached Caps, Polypropylene.5 x x Natural SX4750, SX4750A, PTS-2000, S480.5 x x Blue SX4750, SX4750A, PTS-2000, S480.5 x x Green SX4750, SX4750A, PTS-2000, S480.5 x x Yellow SX4750, SX4750A, PTS-2000, S480.5 x x Orange SX4750, SX4750A, PTS-2000, S480 Tubes with Snap-On Separate Caps, Polypropylene x x F202, F2402H, F24.5, F24.5P, F3602, H x x F202, F2402H, H x x Natural F202, F2402H, F24.5, F24.5P, F30.5, F3602, FX30.5, SX4750, SX4750A, H6002, S24.5, SX24.5, S480, TA-5-.5, FX x x F0850, SX4750, SX4750A, TA-0-250, TA-4-50 Conical Tubes x x (polycarbonate, F0650, F0850, SX4750, SX4750A, FX600, TA-4-50 graduated) x x (polycarbonate SX4750, SX4750A, TA bottle with screw cap) x x (polypropylene SX4750, SX4750A, TA bottle with screw cap) Stainless Steel Tubes x x SX4750, SX4750A, FX600 Bio-Vial Tubes x x Polypropylene SX4750, SX4750A Bottles with Caps, Polycarbonate x x SX4750 Bottles with Cap Assemblies, Polyallomer x x F0650, F0850, SX4750A, TA-0-250, TA-4-50, SX x x F0650, F0850, SX4750A, TA-0-250, TA-4-50, SX * Nominal fi ll volume for these tubes can vary from 6.0 to 0.0 ml, depending on the rotor in which it is used due to different tube angles. 3-9

62 Tubes and Bottles Nominal Nominal Inches Part Rotors Filling Size No. Capacity (ml) (mm) Bottles with Cap Assemblies, Polycarbonate x 89.0 x F x x SX4750, SX4750A x x F0650, F0850, SX4750A, TA-0-250, TA x 02.5 x F0485, F0685, FX x 04.5 x (high-speed cap) F0485, F0685, S480, FX x x (wide mouth) SX4750 Bottles with Cap Assemblies, Polypropylene x x (wide mouth) SX x x (wide mouth) SX4750, TA-0-250, TS x x SX4750 Bottles with Screw Caps, Polyallomer x x F00, S040, S480, TS x 92.0 x F x x F0485, F0650, F0685, F0850, SX4750, SX4750A, TA-0-250, TA-4-50, FX600 Bottles with Screw Caps, Polycarbonate x x F00, S040, S480, TS x 92.0 x F x x F0485, F0650, F0685, F0850, SX4750, SX4750A, TA-0-250, TA-4-50, FX x 04.5 x F0485, S x x (conical) SX4750, SX4750A, TA x x (wide mouth) SX4750, SX4750A, TA-0-250, TS x x SX x x TS x x SX4750 Bottles with Screw Caps, Polypropylene x 04.5 x F0685, FX x 04.5 x F0485, S x 02.5 x F0485, F0685, FX x x S x x (conical) SX4750, SX4750A, TA x x SX x x TS x x SX4750 Bottles with Screw Caps, Teflon x 8.25 x F00, S x x F0650, F0850, SX4750, SX4750A 3 3-0

63 Tools and Supplies 4 4-

64 Tools and Supplies Rotor Cleaning Kit Rotor Cleaning Kit. Contains two 946-mL bottles of Solution 555 Rotor Cleaning Concentrate, Rotor Cleaning Brush, and Rotor Cleaning Brush Replacement Parts/Supplies Solution 555 Rotor Cleaning Concentrate (min. order two Bottles) Rotor Cleaning Brush, 5 8-in. (6 mm) and -in. (25.4 mm), for Rotor Cavity diameters from 7 6-in. ( mm) to -in. (25.4 mm) (min. order three Brushes) Rotor Cleaning Brush, 4-in. (32 mm) and 2-in. (38 mm), for Rotor Cavity diameters from -in. (25.4 mm) to 2-in. (38 mm) (min. order three Brushes) 4 4-2