SPECTROMETERS USER MANUAL. Ver. 1.2_09/16. Making spectroscopy brighter
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1 SPECTROMETERS USER MANUAL Making spectroscopy brighter
2 1 Table of contents 1. General Safety Rules Items Supplied Introduction Installation and Operation Notes Calibration Maintenance Product Versions SPEC STD SPEC STD Specifications Table SPEC RES SPEC RES+ Specifications Table SPEC SENSE SPEC SENSE+ Specifications Table SPEC SPEED SPEC SPEED+ Specifications Table SPEC NIR SPEC NIR Specifications Table Typical Applications Absorbance Reflectance Fluorescence Warranty... 22
3 2 1. General Safety Rules IMPORTANT SAFETY NOTE - Before operating the device, please read carefully this User Manual and be familiar with its contents prior to using this equipment. Do not open the equipment or contact the printed circuit board. Never touch the components or their connections. The printed circuit board is assembled with electrostatically sensitive devices (ESD). Connect the system to the correct power source and use proper optical fibres with SMA905 connectors. Do not spill liquids in the equipment or use it in damp or harsh environments. Never use the equipment near flammable items. Be aware about the danger of electrical shock if not used properly. 2. Items Supplied Spectrometer USB cable AC power adapter (optional) User Manual Calibration Certificate Software
4 3 3. Introduction Thank you for purchasing a Sarspec s spectrometer. Sarspec s spectrometers are designed and optimized for UV/Vis/NIR applications, offering solutions for general purpose spectroscopy with the SPEC STD, high optical resolution (up to 0.2 nm) measurements with the SPEC RES+, low light level measurements with the SPEC SENSE+ and fast acquisition rate (up to 2857 spectra/sec) with the SPEC SPEED+. The flexibility of the SPEC range spectrometers allows to perform multiple applications with a single instrument and with a universal SMA905 connector using optical fibers to direct and collect light from basically everywhere without complex optical systems or significant attenuation. Also available in all models are trigger In/Out for synchronization with other devices like light sources or signal generators and in some models power supply and data transfer are made by a single USB 2.0 connection. All SPEC series spectrometers come as Standard configurations, with listed specifications, for different wavelength ranges/resolution/application or can be user configured. For the user configured spectrometers almost every component can be chosen to perfect match the user s application. Support from Sarspec s specialists is always available for the best configuration/performance. Every spectrometer is supplied with the powerful LightScan or LightScan+ software, including full modules for parameters control, data acquisition and processing. Combine SPEC s series with Light Sources, Optical Fibers, Probes and Accessories to get the right solution for your application.
5 4 4. Installation and Operation NOTE: Install first the device drivers and the Software before USB connection. Please refer to the LightScan Manual or Quick Start. 1. Unpack the spectrometer and remove all the protective plastic. 2. Place it in a clean and flat surface. 3. Install the device drivers and the software on the PC. Please refer to the Software Manual or the Quick Start. 4. For the SPEC STD and SPEC RES+ models plug the USB cable from the spectrometer into an available USB 2.0 port on the PC (see figure below). Note: For these models the USB cable is used both for data transfer and power the spectrometer..
6 5 5. For the SPEC Speed+ and SPEC Sense+ models plug the USB cable from the spectrometer into an available USB 2.0 port on the PC. Also plug the power adaptor into a wall outlet and into the 5V socket of the spectrometer (see figure below). 6. For the SPEC NIR model plug the USB cable from the spectrometer into an available USB 2.0 port on the PC, and plug the power adaptor into a wall outlet and into the 24V socket of the spectrometer (figure below). The RS- 485 interface and opto-isolated PLC are disabled. This connections were introduced for future developments.
7 6 7. Remove the protective cap from the SMA905 socket and insert a compatible optical fiber (see figure below). Notice: Use only compatible optical fibers with SMA905 connector, otherwise it could destroy the entrance slit. 8. Run the LightScan/LightScanPlus software previously installed and have full control of the spectrometer. Please refer to the Software Manual.
8 Notes The Sarspec s logo on the top of the spectrometer will lit on once in operation with the software. It can be switched ON/OFF in the Tools Menu. There are two SMA connectors, Trigger IN and Trigger OUT, for the synchronization with external devices as signal generators and light sources. Trigger IN signal should be +5V TTL compatible, the sync occurs in the rising edge and it is possible to set a delay by software. Trigger OUT signal is +5V TTL compatible.
9 8 5. Calibration All spectrometers are factory calibrated before shipping and supplied with a Wavelength Calibration Certificate that includes all calibration parameters. We recommend the recalibration of the spectrometers every year for accurate results. Contact Sarspec at info@sarspec.com for spectrometer recalibration. 6. Maintenance Unplug the spectrometer from any connections and protect the entrance slit with the supplied cap before proceed. Clean the external surfaces using a soft cloth. Do not use any abrasive cleaners. Do not allow any liquid to drip into the unit.
10 9 7. Product Versions Sarspec s spectrometers are available in four different series: SPEC STD, SPEC RES+, SPEC SENSE+, SPEC SPEED+ and SPEC NIR SPEC STD For an affordable and flexible spectrometer the SPEC STD range is the best option. With detection from 180 to 1100 nm, it can be used in Absorbance, Transmittance, Reflectance, Irradiance, Colour and Fluorescence measurements. The general features for SPEC STD range are: Detection from nm w/ 2048 pixels linear detector CCD array Triggering functions for easy integration (trigger in/out and delay) High speed mini USB 2.0 for power and control Also available as an OEM instrument Robust: fully built in aluminium Minimum integration time of 2 ms Portable, fast and small size Software LightScan included SMA 905 fiber connector, no optical alignment required
11 SPEC STD Specifications Table
12 SPEC RES+ For an affordable spectrometer with great resolution Sarspec offers the SPEC RES+ spectrometers range. With the best resolution/wavelength range ratio for a miniature CCD spectrometer the SPEC RES+ is the best option for Absorbance and Transmittance measurements as well for high throughput light source characterization. The general features for SPEC RES+ range are: Detection from 180 to 1100 nm 3648 pixels linear array CCD detector Great optical resolution of 0.2 nm in a standard configuration Triggering functions for easy integration (trigger in/out and delay) Portable, fast and small size High speed mini USB 2.0 for power and control Also available as an OEM instrument SMA 905 fiber connector, no optical alignment required Robust: fully built in aluminium LED Backlight indicator
13 SPEC RES+ Specifications Table
14 SPEC SENSE+ The SPEC SENSE+ is a spectrometer specially built for low light level measurements such as Fluorescence and Raman but it is also flexible and can be used in Absorbance, Transmittance, Irradiance and Reflectance measurements. The general features for SPEC SENSE+ range are: Largest sensitive area, using 1000 µm height pixels Detection from nm Great performance in the UV SMA 905 fiber connector, no optical alignment required Software LightScan included Fully built in aluminium Back thinned CCD array detector with 2048 pixels Great sensitivity and quantum efficiency up to 78% Triggering functions for easy integration and synchronization High speed mini USB for full control of the spectrometer Minimum integration time of 1 ms Led backlight indicator
15 SPEC SENSE+ Specifications Table
16 SPEC SPEED+ With minimum integration time of 2 µs the SPEC SPEED+ is faster than any other spectrometer available using a CCD array for detection. It was specially built for microsecond s events including fast kinetics in chemical reactions, ignition processes and fluorescence monitoring among a large number of applications that need fast analysis. The general features for SPEC SPEED+ range are: Fast acquisition rate up to 2857 spectra per second using burst mode Back thinned CCD array detector with 2048 pixels Detection from nm Trigger functions for easy integration and synchronization High speed mini USB for full control of the spectrometer Fully built in Aluminium with LED backlight indicator 2 µs integration time with electronic shutter Largest sensitive area, using 1000 µm height pixels Great sensitivity and quantum efficiency up to 78% SMA 905 fiber connector, no optical alignment required Software LightScan+ Included
17 SPEC SPEED+ Specifications Table
18 SPEC NIR The SPEC NIR is based on the principle of scanning grating spectrometers. Radiation passes an entry slit strikes a diffraction grating and is decomposed into its spectral components. The selection of the wavelengths is done by a micro-mechanical mirror (MEMS) which leads the radiation entered to the grating while varying its angle of incidence. Combining an innovative, ultra-fast micro electrical-mechanical mirror and single element detectors for a high performance and affordable solution. The general features for SPEC NIR range are: Detection from 660 to 2400nm TE cooled sensors Fast Scan Time up to 4ms Compact, portable and affordable Robust: fully built in aluminium Allows the combination of Si and InGaAs detectors in one Best Optical Resolution in the market SMA 905 fiber connector, no optical alignment required Great solution for in-line applications Best Price Software LightScan Included
19 SPEC NIR Specifications Table SPEC NIR 1.7 SPEC NIR 2.1 SPEC NIR 2.4 Wavelength Range (nm) Average Spectral Resolution ~ 3nm (150um slit) ~ 4nm (150um slit) Detectors Si and InGaAs 2 x InGaAs TE Cooling No (Optional) -10 ºC (Optional Uncooled) Signal / Noise 7000:1 4000:1 3600:1 Max Spectra Minute 80 Interface Software USB and RS-485 LightScan (control, acquisition and data handling) Operating System Win XP / Win 7 / Win 8 / Win 8.1 / Win 10 Fiber Connector Dimensions Weight Stray Light Wavelength Accuracy Scan Time Slit Width (options) Power Supply Power Consumption SMA 150 (W) x 100 (D) x 75 (H) mm 1 Kg -30 db <1 nm ±0.1 nm short time jitter 4 ms 300 um, 350 um, 250 um, 200 um, 150 um 24 V 2.9 W (no TE) 5 W (2 TE one for each sensor) Maximum Points per Scan 1481 Order Sorting Filter Detectors are blocked individually by order sorting filters Part Number SP-NIR-1.7 SP-NIR-2.1 SP-NIR-2.4 Options SP-NIR-1.1-C (Cooled) SP-NIR-2.1-U (Uncooled) SP-NIR-2.4-U (Uncooled)
20 19 8. Typical Applications 8.1. Absorbance As full pack for Absorbance measurements all SPEC Packs are configured with wide range UV/Vis/NIR spectrometers, Light Source DW (Deuterium Tungsten) and different accessories depending on the application. The image below refers to the Absorbance SPEC PACK CUV (for cuvettes). Other options available include the Absorbance SPEC PACK PROBE for Absorbance measurements using Transmittance Probes in reaction vessels and the Absorbance SPEC PACK FLOW for Absorbance measurements using flow cells.
21 Reflectance The general configuration consists of a SPEC STD spectrometer configured with 25 µm slit, light source, reflectance probe, probe holder and reflectance standard. LightScan software is also included in this package with a specific module for Reflectance measurements allowing Calibration, Multi-spectra, Quantification, etc. One important feature is that reflectance measurements can be performed with time. This configuration can be purchased for the visible range using a Tungsten Light Source (LS-W) and Vis/NIR SPEC STD Spectrometer or for the Ultraviolet/Visible range using a Deuterium Tungsten High Power and SPEC STD Spectrometer.
22 Fluorescence When performing Fluorescence measurements, in most applications, sensitivity is a key factor and at Sarspec we take it seriously. The configurations offered takes the sensitivity to a new level. By using back thinned detectors with quantum efficiency up to 78% and 1 mm height pixel elements combined with large 200 µm slits and collecting lens make all the difference in the detection of fluorescence signal. Combining the detector with high power LEDs from LS-LED Light Source - with easily interchangeable LEDs - customers can have a sensitive and flexible solution for a fraction of the cost of a traditional spectrofluorimeter. For UV excitation we recommend the Xenon Flashlamp Light Source LS-XeF. The configuration from the image below is a Fluorescence SPEC PACK CUV (for cuvettes), please visit for the available configurations.
23 22 9. Warranty This equipment has one year warranty. Any complaint relating to defects must be submitted in writing without undue delay and shall contain a detailed description of the defect. Warranty may be voided by any of the following: Improper installation or application of instrumentation; use with improper powering; improper system design, disassembling and tampering, exposure to excessive heat, moisture, dirt, dust, corrosion, or any other condition deemed deleterious by Sarspec.
24 23 SPECTROMETERS LIGHT SOURCES OPTICAL FIBERS FIBER OPTIC PROBES SAMPLING ACCESSORIES ABSORBANCE TRANSMITTANCE REFLECTANCE FLUORESCENCE IRRADIANCE RAMAN COLOR MEASUREMENT Sarspec, Lda Rua 28 de Janeiro 350 B Vila Nova de Gaia Portugal Tel Mail.: Web.:
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