Research Article Establishing the Safety of Powder Henna Hair Colour Through Identification of Bandrowski's Base by HPLC Method

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2 OPEN ACCESS Asian Journal of Dermatology ISSN DOI:.3923/ajd Research Article Establishing the Safety of Powder Henna Hair Colour Through Identification of Bandrowski's Base by HPLC ethod Jayaganesh Sankar, Sudhir Sawarkar, Zulfiqar Ali uhammad Anwar and Jainendra alakar Dabur Research and Development, International Business Development, Dubai, P.O. Box 6399, ed Arab Emirates

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5 3 2 standard mg LG (%) BB base mg LG BB (%)

6 mg LG mg LG BB (%) CaO 2+2H2O 2Ca(OH) 2+O2

7 2 (a) mg LG akr 5 (b) mg LG akr 25 (c) mg LG akr

8 25 (d) mg LG 25 (e) mg LG akr 3 (f) mg LG akr

9 5 (a) mg LG (b) mg LG mg LG 2 (c) mg LG

10 25 (d) mg LG 2 (e) mg LG (f) 2.34 Derector A channel 254 nm mg LG

11 2 (a) mg LG mg LG (%) (b) mg LG mg LG BB (%) (c) mg LG mg LG BB (%)

12 25 (d) mg LG mg LG (e) (%) mg LG mg LG BB (f) (%) mg LG mg LG BB (%)

13 2 (a) mg LG akr 5 (b) mg LG akr 25 (c) mg LG akr

14 25 (d) Derector A channel 254 nm mg LG akr 25 (e) Derector A channel 254 nm mg LG akr 3 (f) Derector A channel 254 nm mg LG akr

15 H.. N H Step Oxidation Step 2 :N r H H. HO O Resorcional anion s HN 2 N OH O Indophenol Step 4 [I] H N H H N OH O (XXVIII) Step 5 [O] Step 3 [O] Indophenol Indophenol (XXVII) [O] [O] H H N OH O Indophenol [O] Brown polymeric polyindophenols N NH r NH NH p-phenylenediamine HN 2 N Bandrowski's Base

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