xponent Version 4.2 RELEASE NOTES

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1 xponent Version 4.2 RELEASE NOTES Copyright Luminex Corporation, All rights reserved. Part Number: Disclaimer Subject to change without notice. Although prepared to ensure accuracy, Luminex assumes no liability for errors or omissions, or for any damages resulting from the application or use of this information. Support and Contact Information To report other issues, contact Luminex Technical Support between the hours of 7:00 a.m. and 7:00 p.m. Central Time, Monday through Friday: - Inside the U.S. and Canada, call Outside the U.S. and Canada, call Or send to support@luminexcorp.com LUMINEX CORPORATION Technology Boulevard Austin, TX U.S.A. Voice: (512) Fax: (512) Installation Notes Please see the installation instructions. Ensure all external storage devices are disconnected. The screen resolution, colors, font size, and theme may affect the xponent display. If the graphics or text is not displayed properly, please adjust your display settings to the recommended system requirements. If upgrading from a previous xponent installation, ensure the license file is backed up from the previous version. Always perform a probe height adjustment after installation. Warning: Modifying or deleting any files installed by xponent or the computer operating system may cause corruption or failure of the xponent software. Modified or deleted xponent system files may be repaired by uninstalling and reinstalling the software. Warning: Use of unauthorized third party software with xponent software may result in corruption or failure of the xponent software and is done at the user s own risk. System Requirements: Recommended: Windows 7 Professional SP1 US English 64-bit (32-bit minimum) 2.8 GHz Intel Core 2 (or higher) 4 GB RAM 160 GB Hard drive space (or higher) Display Settings: SXGA 1280X1024 with 32 bit color 96 DPI Font Default Windows 7 Theme Page 1 of 6

2 SQL Server Express: This setup installs SQL Server Express 2008 R2. Any previous version of SQL Server will not be upgraded to this release. Any currently installed services that utilize SQL Server may become inoperable. Licensing The xponent application is licensed in trial mode for 30 days. The software should be fully licensed or uninstalled before the trial period expires. If the trial period expires, the application will become inoperable. Please contact Luminex technical support to obtain a permanent license. See the xponent license agreement for more information. Documentation Notes To access the most recent information regarding xponent, refer to our website at Application Notes 1. If a batch spans more than one plate, the plate tray will eject automatically at the completion of a plate in anticipation of the next plate in the batch. A dialog box will display prompting you to insert the next plate. 2. Do not select a CD, DVD, or external drive for the archive location. Archive the data to a local folder first and then copy it to the CD, DVD, or external drive. 3. Use an external USB hub, such as the ports on the PC monitor, for connecting and disconnecting USB devices. Directly accessing the USB ports on the PC may cause the system to stop responding and the device and software may need to be restarted. 4. If backgrounds and/or standards are not placed at the beginning of plate, data analysis may take longer than expected. Please allow an adequate amount of time for analysis to complete. 5. If you experience performance issues, 'out-of-memory' issues, 'C++' errors, exceptions, or general system errors or issues, restart the PC and device before performing another operation. 6. If you terminate the xponent application using the Task Manager because a process is not responding, you must restart the PC before launching xponent again. Please give operations on large batches adequate time to complete before abnormally terminating xponent. 7. All folder locations set up in the Admin menu must have read and write privileges for all Windows login users on the system. 8. If a failure to detach error occurs when importing or exporting files, the error may be ignored. 9. If you are importing, exporting, saving, generating reports or performing analysis on large batches, please allow adequate time for the operation to complete. 10. When running a multi-batch, ensure each batch utilizes the same heater settings. 11. While performing an operation, such as running a batch, avoid performing other operations, such as archive, export, import, opening saved batches, and creating protocols or batches. 12. Reports should be saved and viewed in PDF format; other types may not be formatted correctly. 13. Microsoft Internet Explorer should be used for Remote Web Monitoring. 14. Avoid scheduling automatic archive operations or manually archiving when xponent will be in use, such as when running batches. 15. When creating multi-batches, the first batch added will retain the starting position of the individual batch. For subsequently added batches, the first acquisition will be moved to the selected well on the plate layout graphic in the multi-batch page. 16. Use the Next and Back buttons rather than directly selecting sub-tabs when performing stepwise operations. 17. When changing PC regional settings, it may be necessary to adjust the registry. Always restart the PC after changing regional settings and adjusting the registry. Page 2 of 6

3 18. If an exported file cannot be moved or copied, it may be necessary to restart xponent. 19. Invalid test results may be indicated as "NaN" (Not a Number), NA, or be blank; all which indicate that a valid test result could not be calculated. 20. If importing protocols from previous versions of xponent, examine the protocol for correctness after importing. 21. Use a local directory, such as My Documents, when importing or exporting files rather than directly accessing remote locations from the application. Remaining Issues The following issues were noted prior to the release of Luminex xponent 4.2. Please review these issues carefully before running the Luminex xponent application. 1. An error may occur when running the setup from the setup.html file if not using Internet Explorer. I3581 Workaround: Use Internet Explorer to open setup.html. 2. The Cut function in the right-click menu of the Std/Ctrl Details page may not work as expected. I3163 Workaround: Use the copy function and delete the existing data. 3. xponent remains in an aborting state indefinitely if the green laser fails to stabilize. I3286 Workaround: Restart the instrument and the PC 4. When viewing a saved multi-plate batch, the dot plot may not always be updated when switching between plate views. I3309 Workaround: After switching plates, select a well in order to update the dot plot. 5. An error may occur during replay if the instrument is disconnected. I3385 Workaround: Ensure the instrument is connected throughout the replay or disconnect the instrument before starting a replay and keep it disconnected. Alternately, use the recalculate option. 6. When setting the plate tray temperature using the automation interface, the xponent GUI is not updated to reflect the setting until navigating away from the page. I3568 Workaround: Navigate away from the XY heater page and then back in order to see the update. 7. System may run additional wells after an abort is requested due to a low bead count detection batch option threshold. I3960 Workaround: Enter one less well in threshold setting if this occurs. 8. Test results may be listed twice for background samples in a Patient Report. I3606 Workaround: The duplicate test results for background samples can be ignored. 9. Importing files from external sources may not work properly in all cases. I3625 Workaround: Copy the files to the local PC and retry the import. 10. When replaying a batch, the Batch Complete dialog may appear before the replay is actually completed. I3633 Workaround: Wait until all the data in the data grid is finished populating to ensure the batch is completed. 11. If the instrument is not warmed up, the second plate of a multi-plate batch may be aborted. I3647 Workaround: Warmup the instrument prior to running. 12. When replaying a multi-plate batch, it may be necessary to click the Resume button after each plate. I3672 Page 3 of 6

4 Workaround: Click the Resume button after each plate. 13. If an invalid value is entered in the plate layout page and a right-click is performed in the replicate count of commands menu, an invalid copy dialog may be shown. I3911 Workaround: Correct the invalid field before continuing with other operations. 14. Unable to deselect a normalization bead when replaying an allele batch. I3916 Workaround: Ungroup an allele group, deselect the normalization bead, and then regroup the allele group. 15. An exception may occur if a step loss error or collision occurs. I3927 Workaround: Restart the PC and instrument when a step loss error or collision occurs. 16. When switching to 3D view for a large plex batch, the operation may take several minutes. I3936 Workaround: Allow an adequate amount of time for the 3D page to load. 17. If an error occurs during a maintenance routine, a subsequent well may be listed as succeeded in the command list. I3950 Workaround: Only the on-screen display is affected. Use the system log or command report to determine the proper status of the command. 18. Clicking on a well location for a multi-batch plate with horizontal run direction will not select the batch. I3953 Workaround: Select the batch in the multi-batch list. 19. If a sample is deleted from the plate layout, associated maintenance commands may continue to be displayed on the off plate area graphic. I3743 Workaround: The command has been deleted. Selecting another well will update the graphic. 20. If an error occurs during the last well of a batch, the system may remain in a processing state. I3996 Workaround: Restart the PC and then continue. 21. If the probe is obstructed during a probe down command, you must restart the PC. I3392 Workaround: Restart the PC and instrument whenever the probe has been obstructed. 22. Users without permission to "Create, delete, activate Cal and Ver Lots and Kits" may be able to activate individual Cal and Ver lots. I4110 Workaround: Delete the individual Cal and Ver lots that should not be activated. 23. Calibration and verification dot plot appears on the current run screen, but the plate progress and data grid may continue to display information from the previous batch. I5533 Workaround: View the calibration or verification report to view calibration and verification data. 24. An error may occur if a user logs out of Windows prior to exiting the application. LX100/200 Only. I6223 Workaround: Always completely exit the application prior to logging out of Windows 25. When changing the license or group permissions, the settings may not take effect until the PC is restarted. I7511 Workaround: Always restart the PC after changing the license or group permissions. 26. Calibration and Verification lot number may not appear in calibration and verification reports. I7212 Workaround: Always use the Performance Verification report to track kit lot numbers. 27. Files in the process of being archived may still appear on the xponent screen. I7383 Workaround: Do not perform operations on files that are being archived. Allow sufficient time for Page 4 of 6

5 the Archive process to complete. 28. If fluidics commands are part of a maintenance routine that is imported, the fluidics commands will be removed. I7995 Workaround: Use the routines on the Auto-Maint page for performing verification and fluidics tests. 29. Calibration and Verification Kit information may be deleted if upgrading xponent from a previous release. FM3D Only. I8181 Workaround: Reimport the Calibration and Verification Kit files. 30. System restore operation may fail. I8530 Workaround: Restart the PC and attempt the operation again to complete the operation. 31. Running a post well prime command on the last well of a batch may prevent the batch from completing successfully. I8535 Workaround: Do not add a post well prime command to the last well of a batch. The prime command may be included in the post batch routine if required. 32. If a protocol from a previous version of xponent contains a prime command as a post command of the last well of a plate, it may not import the post commands properly. I8536 Workaround: Examine the protocol after importing and edit if necessary. 33. When a user is locked out due to exceeding the number of incorrect password entries, the subsequent message in the system log is incorrect. The error code is "0" and the log type is "Batch" in the system log accessed via Maintenance : System Status. The error code should be non-zero and the log type should be "Security". TFS ID Workaround: Be aware that the System Log error code and log type are incorrect with regard to user lockouts due to incorrect password entries. 34. While editing a Qualitative protocol it is possible to bypass the protection against editing built in formulas. This can occur when changing the name of a user defined formula to the same as a Luminex defined formula. When an attempt is made to save the formula the proper error message appears. However, if the user subsequently edits the formula the Save is enabled and the default formula is overwritten. TFS ID Workaround: Cancel updates or save with a unique name if an error is received for attempting to save a user defined formula with the same name as a Luminex defined formula. 35. While creating a new Qualitative protocol, if a formula is edited for an analyte on the Analysis Settings page valid values are not always accepted after correcting an error. As an example: 1) Click New Formula to make a copy of Lum Qual 2) For Low Positive, change the High Value to be 51. As expected, you now get an error icon (in High Value of High Positive), Save is disabled, and the Preview indicates the error 3) For Low Positive, change the High Value back to a valid value (e.g. 1.1, 40) Even though the values are now valid, the dialog is still in the error state. The error icon is there, Save is disabled, and the Preview indicates an error. TFS ID Workaround: Cancel and then re-copy/re-edit formula. 36. If a new multi-plate protocol is created using a 384-well plate, then the row/column indicator may be incorrect when swapping between plates. FM3D Only. TFS ID Workaround: Be aware that row/column indicator may not match the position indicated on the Plate Navigator page. Subsequent use of the Plate Navigator will correct the issue. 37. During protocol creation, if an invalid DD gating min or max value is entered, two error dialogs appear when clicking the Next button. The first dialog correctly indicates that the gating value is invalid but the second dialog is blank. TFS ID Workaround: Close and disregard the blank second dialog. Page 5 of 6

6 38. Error occurs trying to export protocol that contains two samples with the same ID. TFS ID Workaround: Edit the protocol and assign unique IDs to the samples. 39. System log message for patient import error when entries overlap has import success message instead of error message. TFS ID Workaround: Be aware that system log may not show import error seen on dialog for patient information. Correct file with patient info and re-import. Page 6 of 6

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