SIGMA OLIGO ORDERING GUIDE

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Oligo orders are to be placed by the end user. PO requisitions for oligos should be sent to stores@vet.cam.ac.uk with a screen shot of the order total as a price confirmation. iproc users, please send oligo order requisitions through iproc for approval Non iproc users please register onto the Sigma site: Go to: www.sigmaaldrich.com/united-kingdom.html To register, move the cursor over Hello. Sign In. ACCOUNT and from the drop down menu select New User? Start Here and follow the prompts. Fill in your contact information and ordering information. Where it states Will you be ordering products or requesting quotes online change to YES Fill in Log in information and language preference When you get to account information, please use the following details: Account number: 78097600 PIN: (leave blank) Previous Order #: 8016786619 Ordering Role: Individual Page 1

Fill in the Payment Information billing address. Please ensure the billing address is as shown in the next screen shot, FAO of ACCOUNTS. Please leave the Payment Method section blank then click on Save and Next Page 2

Fill in the Shipping Information shipping address. Please ensure the shipping address is as shown in the next screen shot, FAO of STORES. Then click on Save and Finish to complete registration Please remember your log in details and password as these will be required for every order you place Page 3

Please note: iproc users can use the Sciencewarehouse/Marketplace Sigma punch-out to order oligos. If you are not set up to use iprocurement please go to page 7. Step A (iproc users only). On the marketplace homepage click on Oligos Page 4

Step B (iproc users only). Click on + Create External Sigma Oligo Page 5

Step C (iproc users only). Marketplace will direct you straight to the Sigma site Order Centre, which will look like this iproc users please follow the process onwards from Step 1 on the next page. When it comes to checking out the cart, instead of requesting a PO number from stores you will be diverted back to Marketplace to complete the checkout process as normal and will forward the requisition for approval. Page 6

Non iproc users please go to: www.sigmaaldrich.com/united-kingdom.html to register. Registration information/details can be found on page 1 of this document. If you are already registered with Sigma, please ensure the correct billing and shipping address and account information are entered. Step 1. Move the cursor over the 200,000+ PRODUCTS tab on the left and select ORDER OLIGOS & PEPTIDES from the drop down menu OR click on Oligo Ordering Page 7

Step 2. Select DNA Oligos modified & non-modified Page 8

Step 3. In the Number of items box please type in how many oligos you would like to order and click Go! Page 9

Step 4. Select the Synthesis Scale from the Apply to all dropdown menu Page 10

Step 5. Select the Purification from the Apply to all dropdown menu. Step 6. Type in any additional specifications you would like to apply to all oligos in the right hand box. Page 11

Step 7. Select any modifications from the 5 Modification dropdown menu Page 12

Step 8. Select any modifications from the 3 Modification dropdown menu Page 13

Step 9. Type each oligo name and sequence into the relevant fields. When you have filled each line/item #, go to the bottom of the page and select the Format (dry or liquid) from the dropdown menu Page 14

Step 10. Select the Concentration (µm) in water from the dropdown menu Page 15

Step 11. Select the Number of Tubes per Oligo from the dropdown menu Step 12(a). Check the box next to Select All Items and select Preview Price. Page 16

The preview price screen should show as below with a price title in yellow. If it does not show please see Step 12(b). Page 17

Step 12(b). If a preview price is not shown, you may get a screen such as the one shown below with a red error message The usual way to rectify this issue is to change/increase the Synthesis Scale of the oligo/s which are showing an error and select Preview Price until you have preview prices shown in yellow for all oligos. Page 18

Step 13. Check the box Select All Items and click Add to Cart Page 19

Step 14. Move the cursor to the shopping cart at the top right of the screen and Click on View Cart Page 20

Non-iProc users please go to step 15 on page 23. At this point iproc users will be shown the following screen. Please click on Check Out. NB: Do not worry about the Tax which is showing in the order total screen, you will change the VAT type to ME0% when checking out on iproc which will remove the VAT Page 21

(iproc users only) You will be diverted back to Marketplace (as shown below) to checkout in the normal way, sending the requisition for approval. Please note: oligo orders are Medically Exempt Page 22

Step 15. Select the delivery address, recipient and ensure the billing address is selected for a Non Credit Card Order. Select the electronic invoicing address which processes through the finance division at Greenwich House. Click on Continue to Shopping Cart Page 23

Step 16. Please note at this point non iproc users will need to take a screen shot of the total price to provide the written price confirmation for the stores requisition as shown in Appendix A. on page 25. Once you have your PO number, it is imperative to type STORES in the Shipping Address Attention to: field. Enter the PO number into Payment Information P.O. No.: field. And click on Submit Cart. Page 24

Appendix A. Screen shot of the order total this is required for Stores to generate a PO number for non-iproc orders. Please send a copy of this to stores@vet.cam.ac.uk and quote your research grant code or cost centre for a PO to be processed Page 25