VeritiLink Remote Management Software Installing and Using VeritiLink Software Quick Reference Card For more information on installing VeritiLink Software, see the VeritiLink Remote Management Software Installation Guide (PN 4388492). To access the VeritiLink Software Help, click. System Requirements for and Server Computers Hardware Components (Provided by Customer) Computers, including at least one server. The server can also act as a client. Veriti Thermal Cyclers. RJ 45 Cat 5 Ethernet Cables. Router or hub. Printer (optional).. Table 1 System requirements for the server (requirements are based on estimated system load) Minimum Requirements Component Fewer than 5 clients Average of 10 clients Average of 20 clients Up to 25 thermal cyclers 25 to 50 thermal cyclers 25 or more thermal cyclers Computer Intel Dual Core Processor, 2.0 GHz 1 GB RAM 40 GB Hard Drive Monitor 1024 768 pixels Pentium D processor, 3.0 GHz 2 GB RAM 40 GB Hard Drive Intel Xeon processor, 3.0 GHz 2 GB RAM 160 GB Hard Drive, with Raid 1 support Operating System Microsoft Windows XP Operating System, Service Pack 2 or greater Microsoft Windows Server 2003 Operating System Web Browser Microsoft Internet Explorer, v7.0 or Mozilla Firefox, v2.0 For this configuration, select the Apache Web Server option when you install the VeritiLink Software. Table 2 Minimum requirements for client s Component Minimum Requirements Component Minimum Requirements Computer Pentium 4 processor 512 MB RAM 10/100 NIC with RWU (internal) 1 GB Hard Drive Operating System Microsoft Windows XP Operating System, Service Pack 2 or greater Monitor 1024 768 pixels Web Browser Microsoft Internet Explorer, v7.0 or Mozilla Firefox, v2.0
Setting Up the Veriti Thermal Cyclers After updating the firmware to version 1.2.0 (or greater), prepare the thermal cyclers for use. 1. Connect all instruments to the network. A suggested configuration is shown here: Router VeritiLink Software server 5. Set the IP address. The thermal cycler must have an IP address to be recognized on the network. Choose to have a on the network dynamically assign the IP address or set a static IP address. a. Touch IP Settings. b. Touch Dynamic or Static. For static addresses, also enter the IP address for the thermal cycler, the subnet mask, and the default gateway. c. Touch Done. External (company) network Printer 2. Log in to the thermal cycler as a user with administrative privileges and go to the Admin Menu screen. Touch Settings Menu, then touch Admin Menu. 3. Set the instrument name. By default the instrument name is set to the thermal cycler serial number. You can change the name so it will be easier to identify. a. Touch Set Instrument Name. b. Touch the field to enter the name, then touch Done. 6. Enable VeritiLink Software access to the thermal cycler. a. Touch Security. b. Touch Yes next to Allow VeritiLink Software Control. Change other settings as desired. c. Touch Done. 4. Set the date and time. a. Touch Set Date & Time. b. In the Date and Time screen, touch the fields to enter the date and time, then touch Done. 7. Toggle the switch on the back of the thermal cycler to power off. 8. Toggle the switch again to power on. Page 2
Installing the VeritiLink Software Server Insert the VeritiLink Remote Management Software installation CD into the CD drive and follow the instructions that appear on the screen. In the Select Features screen, check Apache Web Server for laboratories with many thermal cyclers and/or many clients. Starting the VeritiLink Software Server The VeritiLink Software Server is installed as a service and starts automatically after the software is installed and any time the server is restarted. If you need to restart the server manually, follow these instructions: 1. Click Start All Programs Applied Biosystems VeritiLink Remote Management Software Start VRMS Services. 2. To use the software, open your web browser and, in the URL field, enter the VeritiLink Software server IP address or name and then press Enter. Setting Up VeritiLink Remote Management Software Use VeritiLink Remote Management Software Setup Wizard to set up the software. The Setup Wizard appears when the administrator first logs into the VeritiLink Software. Figure 1 Discover Instruments tab in the Setup Wizard, before and after clicking Discover Instruments To identify thermal cyclers available on the network, click Discover Instruments. All thermal cyclers on the network are displayed in the window. Follow the instructions in the Setup Wizard to select instruments for monitoring, to create groups, set up security, verification reminders and email notifications. For any thermal cycler that does not appear in this window, try the following: 1. Verify the thermal cycler is powered on. 2. Verify that the IP address for the thermal cycler is correct. 3. Verify that the Allow VeritiLink Software Control setting (in the Security screen on the thermal cycler) is Yes. 4. Verify the Ethernet cables are connected to the missing thermal cycler and the network. 5. Restart the missing thermal cycler. 6. Click Discover Instruments again. Page 3
Monitoring Thermal Cyclers in the Instruments Tab You can see information about one or more thermal cyclers in the Instruments tab in any of four views. Some views use icons to convey information, as shown in Table 3. Figure 2 Overview view, showing one type of information at a time Figure 3 Single Instrument view, showing details for one instrument at a time Figure 4 Thumbnails view, showing up to 6 types of information at a time Figure 5 Table 3 Detailed List view, showing a tabular view of all thermal cyclers in the system Thermal cycler status icons Status Icon and Meaning Run Status Unavailable: Ready: Starting run: Running: Paused: Stopping run or aborted: Notification Status Enabled: Disabled: Verification Status Reminders disabled: Verifications current: \ Verifications overdue: Page 4 Part Number 4390856 Rev. A
Creating and Editing a Run Method 1. Click the Manage Methods tab. 2. In the menu bar, click Create New Method. The run method editor opens, with the default run method displayed. Using the default run method as a template you can: Add and delete steps and stages Edit the number of cycles in a stage Edit time, temperature, and ramp rate for a step Add a pause before a step Add AutoDelta steps Edit the method name, sample volume, sample block type, and heated cover temperature 3. When you are done, click Save Method to save the run method to your or to a thermal cycler. Performing a Thermal Cycling Run Follow the steps below to perform a run on more than one thermal cycler, and/or when using a method stored on the. 1. In the Instruments tab, select a thermal cycler for the run. Ctrl-click to select additional thermal cyclers with the same or compatible blocks, then click Start to open the Start Run dialog. 2. In the Start Run dialog, choose a run method: For a run method stored on a thermal cycler, click Instrument. Use the Instrument drop-down list to select the thermal cycler, then click a folder to display the run methods. Click the run method in the table to select it. (The run method does not have to be located on the instrument performing the run.) Page 5 Part Number 4390856 Rev. A
For a run method on your, click My Computer, then click Browse. Navigate to the run method, click Open, then Upload. 3. Enter changes to the run parameters. 4. Load your samples in the thermal cyclers (s) and click Start Run. During a Thermal Cycling Run Actions During a Run To... Do This Stop the run Click Abort Pause during the run Click Pause Continue a paused run without waiting for the pause to complete Click Resume Change the number of cycles for any stage Click Modify Run Change Number of Cycles Skip to the next step Click Modify Run Skip to Next Step Viewing the Progress of the Run Click the Instruments tab. Click Single Instrument in the View drop-down list and select the thermal cycler from the Details for Instrument drop-down list. The vertical yellow bar highlights the current step. The time remaining in the run is also shown. Copyright 2007, Applied Biosystems. All rights reserved. For General Laboratory Use. Applera, Applied Biosystems, and AB (Design) are registered trademarks, Veriti and VeritiLink are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries. All other trademarks are the sole property of their respective owners. 12/2007 www.appliedbiosystems.com Part Number 4390856 Rev. A