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1 3 4 5 6 7 8 9 10 11 1 13 14 15 16 17 18 19 0 1 3 4 5 6 7 8 9 30 31 3 33 34 35 36 37 38 Abstract sodales vulputate auctor. Nam lacus felis, fermentum sit amet nulla ac, tristique ultrices tellus. Integer rutrum aliquet sapien, eu fermentum magna pellentesque vitae. Integer semper viverra mauris vel pulvinar. Suspendisse sagittis malesuada urna. Praesent mauris diam, fringilla id fringilla ac, posuere non lorem. Vestibulum mauris ante, fringilla quis tortor sit amet, accumsan fermentum quam. Nulla dictum consectetur leo. Ut vulputate ipsum purus, a interdum nibh viverra et. Praesent aliquam sapien vel massa sodales bibendum. Nulla interdum accumsan lectus, sed auctor elit accumsan a. Suspendisse quis rhoncus nibh. The verum est de illic. Author summary pharetra quam, vitae convallis nunc. Introduction Lorem ipsum dolor sit amet, consectetur adipiscing [1]. interdum. Donec tincidunt porta sem nec hendrerit. Vestibul [-6] sodales vulputate auctor. Nam lacus felis, fermentum sit amet nulla ac, tristique ultrices tellus. Integer rutrum aliquet sapien, eu fermentum magna pellentesque vitae. Integer semper viverra mauris vel pulvinar. Suspendisse sagittis malesuada urna. Praesent mauris diam, fringilla id fringilla ac, posuere non lorem. Vestibulum mauris ante, fringilla quis tortor sit amet, accumsan fermentum quam. Nulla dictum consectetur leo. Ut vulputate ipsum purus, a interdum nibh viverra et. Praesent aliquam sapien vel massa sodales bibendum. Nulla interdum accumsan lectus, sed auctor elit accumsan a. Suspendisse quis rhoncus nibh. The verum est de illic. 1 Level 1 Heading Use Level 1 heading for all major sections (Abstract, Introduction, Methods, Models, Results, Discussion, Acknowledgments, ). Bold type, 14pt font. formatting where needed (e.g. genus and species names, genes, etc.). Headings should be written in sentence case (capitalize only the first word of the heading, the first word of the subheading, and any proper nouns and genus names). NOTE: Do not cite figures, tables, supporting information, or references in the Abstract. Reference Citations Cite references in brackets (for example, [1] or [-5] or [3,7,9] ). References must be cited in order at first mention. NOTE: [Brackets] should not be used for figure, table, or supporting information citations.

39 40 41 4 43 44 45 46 Results Vestibulum adipiscing urna ut lectus gravida, vitae (Fig 1) sodales vulputate auctor. Nam sit amet nulla lacus a, Figs 1 and ultrices tellus. Integer rutrum aliquet sapien, eu fermentum magna pellentesque vitae. Figure Citations Cite figures as Fig 1, Fig, etc. Cite figures and tables in order. Do not cite Fig before Fig 1. Cite multiple figures as Figs 1 and, Figs 1-3, etc. 47 48 49 50 51 5 53 54 55 56 57 58 59 60 61 6 63 64 65 66 67 Fig 1. This is the Fig 1 Title. This is the Fig 1 legend. Fig. This is the Fig Title. This is the Fig legend. File Naming for Figures Figure files should be saved as Fig1.tif, Fig.eps, etc. Acceptable file formats for figures are.tif,.tiff, and.eps Figures should be uploaded separately, as individual files. interdum. Donec p et q tincidunt porta sem nec hendrerit. p pqq 1 (1) Vestibulum nec pharetra quam, vitae convallis nunc. Mauris in mattis sapien. Fusce sodales vulputate auctor. Nam lacus felis, fermentum sit amet nulla ac, tristique ultrices tellus. Integer rutrum aliquet sapien, eu fermentum magna pellentesque vitae. Integer semper viverra mauris vel pulvinar dolor sit amet en ( p q) 1. NOTE: This document is presented in single-space paragraph format for ease of use. Please submit your manuscript in doublespace paragraph format. Figure Captions Display/Numbered Equation Format display equations in Mathtype or Equation Tools. Do not use Graphic Objects. Number equations as (1), (), etc. Inline Equation Each figure caption should appear directly after the paragraph in which they are first cited. Do not include tables within captions. Use bold type for the figure titles. Format in regular text or as an inline equation in Mathtype or Equation Tools. Do not use Symbol Font. Do not use Graphic Objects.

68 69 70 71 7 73 Genotyping interdum. Donec tincidunt porta sem nec hendrerit. Omnes tuum basi sunt pertinent ad nos. Nam lacus felis, fermentum sit amet nulla ac, tristique ultrices tellus. Level Heading Use Level headings for sub-sections of major sections. Bold type, 1pt font. formatting where needed. Use sentence case. 74 75 76 77 78 79 80 81 8 83 84 85 86 87 Whole genome RFLP analysis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum adipiscing urna ut lectus gravida, vitae blandit tortor interdum. Donec tincidunt porta sem nec hendrerit. Vestibulum nec pharetra quam, vitae. Numquam iens dare tibi up. Methods Vestibulum adipiscing urna ut lectus gravida, et bland sit amet donec tincidunt porta sem nec hendrerit. Fido nemo. Vesti Table 1 Table 1. This is the Table 1 Title. 1 3 4 5 W 1W W 3W a 4W 5W X 1X X 3X 4X 5X Y 1Y Y 3Y b 4Y 5Y Z 1Z Z 3Z 4Z 5Z This is the Table 1 legend. a Table footnotes belong here. b Footnotes should each have a corresponding symbol in the table. Level 3 heading Use Level 3 headings for sub-sections within Level headings. Inline, Bold type, 1pt font. formatting where needed. Use sentence case. Tables and Table Citations Tables should be cited as Table 1, Table, etc. Cite multiple tables as Tables 1 and, Tables 1-3, etc. Tables should be included directly after the paragraph in which they are first cited. Tables must be cellbased in Microsoft Word or embedded with Microsoft Excel. Do not use empty rows to create spacing. Do not include graphic objects, images, or colored text. See PLOS NTDs Table Guidelines for more complete instructions 3

88 89 90 91 9 93 94 95 96 97 98 99 100 101 10 103 104 105 106 107 108 109 110 111 11 113 114 115 116 117 118 Lorem ipsum dolor sit amet, consectetur adipiscing dets. Vestibulum adipiscing urna ut lectus gravida, vitae blandit S1 Fig tortor interdum. Donec tincidunt porta sem nec S1 and S Tables hendrerit. Vestibulum nec pharetra quam, vitae convallis nunc. Mauris in mattis sapien. Fusce sodales vulputate auctor. Vestibulum mauris ante, fringilla quis tortor sit amet, accumsan fermentum quam. Nulla dictum consectetur leo. Ut vulputate ipsum purus, a interdum nibh viverra et. Praesent aliquam sapien vel massa sodales bibendum. Nulla interdum accumsan lectus, sed auctor elit accumsan a. Suspendisse quis rhoncus nibh. Acknowledgments interdum. References 1. Doe J, Data A, van Stats J, Testperson M, Ribosome D Jr, McBio GHT, et al. This is the article title. PLoS Negl Trop Dis. 017;11(1):e0000. doi: 10.1371/journal.pntd.0000000. Doe J, Data A, van Stats J, Testperson M, Ribosome D Jr, McBio GHT. Intraspecific competition for food resources in desert-dwelling pikas. PLoS Negl Trop Dis. 017;11(1):e0000. doi:10.1371/journal.pntd.0000000 3. Doe J, Data A, van Stats J, Testperson M, Ribosome D Jr, McBio GHT, et al. Bunny dynamics in cartoon landscapes. PLoS Negl Trop Dis. Forthcoming 017. Citations Format Supporting Information Citations as S1 Fig, S1 Table, etc. Cite multiple files as S1 and S Figs, S1-S3 Figs, etc. It is not required to cite each Supporting Information file. Acknowledgments Do not include funding or competing interests information in Acknowledgments. References References should be listed after the main text, before the supporting information. References with more than six authors should list the first six author names, followed by et al. Please see the PLOS NTDs guide for References here: http://journals.plos.org/ plosntds/s/submissionguidelines#locsupporting-information 119 4

10 11 1 13 14 15 Supporting information S1 Fig. This is the S1 Fig Title. This is the S1 Fig legend. S Fig. This is the S Fig Title. This is the S Fig legend. S1 Table. This is the S1 Table Title. This is the S1 Table legend. S Table. This is the S Table Title. This is the S Table legend. S1 File. This is the S1 File Title. This is the S1 File legend. 16 File Naming for 17 18 files should be saved as S1_Fig.tif, S1_File.pdf, etc. All file types are supported. should be uploaded separately, as individual files. Please see the PLOS NTDs guidelines for Supporting Information here: http://journals.plos.org/plosntds/s/supporting-information Captions List Supporting Information captions at the end of the manuscript in a section titled Supporting information. Use a Level 1 heading. Use bold type for the titles. files do not require full captions, only labels ( S1 Fig ) are required. Please also see the PLOS NTDs Submission Guidelines, which can be found here: http://journals.plos.org/plosntds/s/submission-guidelines For assistance preparing figures, please contact figures@plos.org For assistance with other formatting requirements, contact plosntds@plos.org 5