.slide digital virtual microscopy
.slide animation
.slide products for virtual microscopy TMA scan conferencing.slide Desktop license SL50 Slide Loader Net Image Server OlyVIA OlyVIAWeb (web viewer)
Main focus of.slide.slide: Where is the main focus: Image Quality Speed Upgradeability Flexibility
Which features are necessary? Seamless image stitching and optimal focus Flexible use of the BX light microscopes Selectable resolution e.g. 2x 4x 10x 20x 40x 100x Different observation methods (brightfield, fluorescence,..) Simultaneous processing of many tasks Memory management for very large images Speech, text and drawing annotation High colour reproducibility using ICC profiles Virtual slides accessible from anywhere at any magnification
Digitization example: typical sample viewing with 40x objective microscope can display a small field of view only Y o u r V i s i o n, O u r F u t u r e.slide what are the requirements
Digilization display would need about 9000 Monitors (17 ) image size about 124000 x 76000 pixel image was generated by 4500 single snapshots ~1GB image size an 15GB uncompressed image size.slide what are the requirements ~ 22.5 m ~35m
Multilayer scan with.slide Single layer versus Multi layer scan Conventional virtual slide scanner Olympus.slide system
Multilayer scan with.slide conventional single layer scan 1 scan = 1 image n scans = n images 10x 20x 2x vsi image 40x 10x vsi image 20x vsi image 40x vsi image
Multilayer scan with.slide Multi layer scan n scans = 1 image clear defined position where a high resolution detail scan was taken 10x 20x 40x overview
Virtual z stacks with.slide under development Z stacks focussing on a virtual slide Z stack Z stack
Net Image Server for.slide Net Image Server security with password and user login direct accessing of image files via LAN/WAN Images accessible by :.slide viewer OlyVIA Webbrowser (flash) Not only vsi files can be used, ALL standard image formats Must be able to integrate into existing image databases
OlyVIA Viewer for Imaging Applications OlyVIA viewer software: Remote access to.slide image server via LAN/www convenient: dongle less for free fast downloadable easy to use high speed image transfer over the LAN/WAN high image quality annotations, text and sound available over the LAN/WAN image synchronization even with multiple image server
OlyVIA
Web Viewer OlyVIAWeb viewer software: Remote access to.slide image server via WWW convenient: for free fast no download necessary easy to use MAC compatible high speed image transfer over the WWW high image quality
Web Viewer Demo Server available http://83.236.170.16 5/webimage/webDB. aspx User Name: guest Password: guest Via Olivia
Web Viewer
.slide applications what's the purpose for Digital virtual Microscopy? Education (universities and medical institutions) E learning Student courses & exams reference images Histo Patho Databases
.slide applications what's the purpose for Digital virtual Microscopy? Research cancer research pharmacological research neuroscience research
.slide applications what's the purpose for Digital virtual Microscopy? Research (cancer, pharmacological, neuroscience) High throughput, high content applications TMA (Tissue Micro Array) analysis Image analysis on large field of views
.slide applications what's the purpose for Digital virtual Microscopy? Histology/Pathology laboratories & hospitals Clinical environment, HIS&PACS integration
.slide applications what's the purpose for Digital virtual Microscopy? Histology/Pathology laboratories & hospitals Archiving of rare and valuable samples Collaborative working second opinion & discussion validation of results done by remote located experts Routine pathology workflow
3 main fields of application 1) education 2) clinical Research 3) hospital environment
Internet Y o u r V i s i o n, O u r F u t u r e.slide for education Student courses and examines made on virtual slides Clients e.g. students PC or laptop Communication from the client via LAN to the central Database Teacher
Internet Y o u r V i s i o n, O u r F u t u r e.slide viewing, conferencing / consulting Communication is more important than ever Access to images: anytime anywhere
.slide conferencing / consulting Conferencing (1:N): For education / discussion of images 1 administrator edits the image Annotations Snapshot areas N users watch the scene Administrator can give the edit right to any conference participants Consulting (1:1) ask for a second opinion Workflow: 1. Use annotation tools to: 1. Define interesting areas 2. Ask/answer questions 2. Save image to server 3. Notify discussion partner
.slide at customers site education high performance of the Net Image Server software needed Performance tests have been made by OT : measuring CPU load, disk I/O load and bandwidth load when accessing 25 slides by 25 clients at the same time student exams have been performed with 96 clients at Japan Universities online reference databases have been created in EU (France, Germany, Spain) integration of virtual slides with the Net Image Server into one of the largest European online histo pathology database is under construction the WHO is evaluating to publish their slides with the Net Image Server
Education & consultation scenario I (1 central DB server & 1 file server) Olympus.slide workstation Central service n hospitals SQL DBServer & Image repository Internet or private Network Olympus.slide workstation Olympus.slide workstation hospital X hospital Z Olympus.slide workstation hospital Y
Education & consultation scenario II (one DB server multiple file server) Olympus.slide workstation Central service SQL DBServer & Image repository n hospitals Olympus.slide workstation Internet or private Network Olympus.slide workstation hospital X NetImage Server file server image repository NetImage Server file server Image repository hospital Z NetImage Server file server Image repository Olympus.slide workstation hospital Y
.slide at customers site research high flexibility due to different applications different focus algorithm necessary compatible file formats Image processing (geometrical functions) EFI and z stacks fluorescence illumination needed Neuropathology research TMA scan for cancer research Pharmacological companies
.slide in routine hospital environment under evaluation Complete integrated solution for pathology institutes Integration of.slide via HL7 / DICOM Open vsi images of a specific patient from the patient linked database Cross talk between.slide image database and patient linked database cooperation with the market leader in each country is necessary
.slide at customers site clinical routine under heavy discussions at the moment due to incompatibility of whole slide image file formats waiting until DICOM modality for pathology is defined only pilot projects have been implemented as research & evaluation projects with some hospitals
Image Database for pathology under evaluation Image acquisition and insertion to Database Scanning samples with.slide Assignment of Case ID (manual or via Barcode) Collect patient data via HL7 (Patient, case, etc) Automatically. insert Images and metadata to DB pathology Information system Image assessment Image Database Retrieval of image of interest (+reference images Virtual microscopy With.slide Diagnosis input Update of data In image DB And in PIS(HL7)
.slide specs&info optical unit: BX2 microscopy technology with selectable magnification. High resolution objectives, UPLSAPO 10x (N.A. 0.16), 20x (N.A. 0,75), 40x (N.A. 0.9) CCD camera: peltier cooled 2/3 CCD camera with 6.45 µm pixel size Workstation: high performance dual Xeon workstation with 500GB data storage capacity 20.1 high resolution TFT monitor typical scan speed for 10mm x 10mm sample with 20x objective: less than 1.5 min with standard resolution (0,5µm/pixel) optional: high resolution scan 0.32µm/pixel, less than 3 min selectable image compression during scanning: Data compression takes place during the scanning process without any time delay. The compression ratio and compression type, like like jpg, jpg2000, can be adjusted or even switched off. Focus map Focus points are taken automatically and only where the tissue is. Different focus map algorithm assure optimal image quality Tissue finding Automatic
.slide specs&info Resolution&image size Objective UIS2 (N. A.) 2 (0.08) 10 (0.16) 20 (0.75) 40 (0.90) TV 0.63x TV 1.0x TV 0.63x TV 1.0x TV 0.63x TV 1.0x TV 0.63x TV 1.0x pixel resolution [µm] 2.03 3.22 1.02 0.64 0.51 0.32 0.26 0.16 Uncompressed image size [10x10mm] ~11MB ~29MB ~0.3 GB ~0.7 GB ~1.2GB ~2.9GB ~4.6GB 11.6GB Image size high image quality low compression Image size standard image quality high compression ~1.2MB ~2.9MB ~ 28MB ~72MB ~110MB ~290MB ~260MB ~1.2GB ~0.7MB ~1.5MB ~14MB ~36MB ~60MB ~150MB ~130MB ~0.6GB
Slide Loader Integration Slide loader system with integrated bar code reader
Slide Loader Fully automatic batch mode for 50 slide Individual setting (scan option, magnification) for each slide possible Automatic bar code recognition
Storage Solution for.slide