Andreas Reimann Berlin im Juli 2009

Agilent Technologies ASMS 2009 Andreas Reimann Berlin im Juli 2009

New Triple Quad LC/MS Portfolio 6410, 6430 & 6460 Page 2

Comparison of 6400 Series QQQs 6410BA 6430 6460 Sensitivity: 1 pg reserpine S/N > 150:1 S/N > 300:1 S/N > 1,000:1 1xRMS 1xRMS 1xRMS Mass Range 5 2,000 amu 5 2,250 amu 5 3,000 amu Minimum Dwell Time 5 msec 1 msec 1 msec Polarity Switching 250 msec* 30 msec 30 msec Scan Speed 5,200 u/sec 5,200 u/sec 5,200 u/sec No. of MRMs 10,000 20,000 20,000 Dynamic MRMs 4,000 4,000 4,000 Mass Resolution 0.7 Da 0.5 Da 0.5Da *New Polarity Switching Specification for the 6410BA in August Page 3 Page 3

The new 6430 Triple Quad: ideal QQQ for ChipCube <10 attomole sensitivity 6430 Triple Quad LC/MS HPLC Chip System Page 4

2,000 DMRM transitions for tryptic peptides in depleted human plasma with 6400 Series QQQ Page 5

Collaboration with a leader in Biomarker Research SANTA CLARA, Calif., and WASHINGTON, May 26, 2009 Agilent Technologies Inc. (NYSE: A) and Anderson Forschung Group LLC (AFG) today announced a collaboration to develop quantitative peptide assays to speed protein biomarker discovery and validation. Leigh Anderson, CEO of the Plasma Proteome Institute tute The collaboration will combine AFG s stable isotope standards and capture by anti-peptide antibodies (SISCAPA) technology with Agilent s 1200 Series HPLC- Chip and 6400 Series triple quadrupole mass spectrometers (MS) SISCAPA assays for candidate biomarkers can benefit substantially from the reproducibility and sensitivity of Agilent s platform, and we look forward to optimizing this combination, said Leigh Anderson, AFG s chief executive officer. Page 6

Agilent 6540 Ultra High Definition (UHD) Accurate Mass Q-TOF LC/MS Exceptional accurate mass, sensitivity, dynamic range and resolution perfect match for 1290 Infinity UHPLC Designed for high confidence analysis of complex samples

Ultra High Definition Optimizing all Analytical Dimensions Signal Response Sensitivity Dynamic Range Linearity Separation Speed Peak Capacity Mass Spectrum Mass Accuracy Resolving Power Acquisition Rate

Agilent Q-TOF Family 6530AA Q-TOF - 20K resolution - 1 pg sensitivity - 5 decades in-scan 6520AA Q-TOF 2008 2009 6540AA/ 6538 Q-TOF -40K resolution/ - < 1 PPM MS, 3 PPM MS/MS -2pg sensitivity - 5 decades in-scan Shipping Oct 2009 6530AA Q-TOF - 20K resolution - 20K resolution - 5 Pg Sensitivity - 1 pg sensitivity - 5 decades in-scan - 5 decades in-scan 6510AA Q-TOF - 13K resolution - 3.5 decades in-scan - 5 Pg Sensitivity - Obsolete June 2009 6520BA Q-TOF - 20K resolution - 5 Pg Sensitivity - 5 decades in-scan Page 9

Agilent 6540 Ultra High Definition Accurate Mass Q-TOF 500 ppb mass accuracy femtogram sensitivity 5 decades dynamic range 40,000 resolving power 20 Spectra/sec Excellent Linearity and Isotopic Fidelity Supports Agilent Jet Stream and Supports Agilent Jet Stream and HPLC-Chip

Ion Beam Compression (IBC)* Technology Drives Higher Resolution Compressed and cooled ion beam ensures the best sensitivity performance in high resolution mode Exit from collision cell Into slicer and pulser region Narrowed beam slit enables mass resolution of 40K Up to 10-fold ion beam compression * Patent pending

Enhanced Mirror Technology (EMT) Enhanced Mirror Technology minimizes variation and distribution of ion arrival time and energies 2nd order time focusing Deeper electrostatic ion mirror 35% longer flight tube

Next Generation Ultra High Speed Detector New Bipolar TOF Detector New ultra fast and high efficiency scintillator New ultra fast response PMT design continues the tradition of high dynamic range and detector lifetime Developed by Photonis with Agilent TOF Technology Specifically enhances Resolution in 2Ghz Ext. Dynamic Range Mode 2 nsec/div Single Ion Response ~800 psec FWHM Making Research Grade Performance possible in a Benchtop Format

Ultra High Speed Acquisition From Agilent s Leadership in GHz Speed Electronics 4 GHz Acquisition for Maximum Resolving Power and <1ppm Mass Accuracy 5 Decades of in-spectrum Dynamic Range from 2-Channel x 2 GHz Dual Gain Mode Dual Input Agilent pre-amplifiers Picture of 4GHz board Goes here 4 GHz (8 bit) Analog-Digital-Converter Adapted from Agilent s High Speed Oscilloscope Systems Ultra High Speed FPGAs process and store transients in real time FPGAs 4 GHz Agilent ADC Making Research Grade Performance possible in a Benchtop Format

6540 Ultra High Definition Q-TOF Key Design Enhancements Feature Advantage Benefit Ion Beam Compressioncompresses ion beam into dense layer with uniform energy Enhanced Mirror Technologydeeper mirror with 2 nd order focusing Longer Flight Tube 35% increase in length Fast Bipolar Detector 2X faster than previous version Fine tune Internal Reference Based Calibration Minimize sensitivity loss while passing through narrow slit Minimize distribution of ion arrival time and energy Added length provides more time for ions to resolve Greater mass resolution in extended dynamic range mode 2-3 fold improvement in mass accuracy Greater mass resolution without sensitivity loss Greater mass resolution Greater mass resolution ID more compounds in complex mixtures- metabolomics Greater confidence for compound ID

Enhancements in Resolving Power High Resolution and Ext. Dynamic Range Modes r Resolvin ng Powe 45000 40000 35000 30000 25000 20000 15000 10000 5000 0 6540 High Resolution 6540 Ext. Dynamic Range 6530 High Resolution 6530 0 500 1000 1500 2000 2500 3000m/z Ext. Dynamic Range

6540 Ultra High Definition QTOF Maintaining i i Resolving Power Across the Mass Range 622.00822 m/z 622 and isotopes 922 5 55 5.5 622 R=42424 623.01059 x10 R 42424 R=38702 5 4.5 4 3.5 3 322 2.5 R=30218 1221 R=43674 1521 R=42750 2121 1821 R=41825 2 R=40405 1.5 1 0.5 Counts vs. Mass-to-Charge (m/z) 624.01219 625.01471 2421 R=39332 2721 R=37207 0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 Counts vs. Mass-to-Charge (m/z) Scan Rate Independent d 17 Orbitrap evaluation March 2009

6540 Ultra High Definition QTOF Mass Accuracy Repetitive Injections 40pg reserpine on-column, 10 injections +ESI EIC(609.28066) Scan Frag=240.0V Reserpine_40pgms3.d 0.511 0.0 0.25 0.5 0.75 1 Counts vs. Acquisition Time (min) Error Run (ppm) 1 0.96 2 0.17 3 0.25 4 0.02 5 0.39 6 013 0.13 7 0.01 8 0.52 9 0.04 10 0.30 Mean 0.25 Std. Dev 0.32 x10 5 1.2 1 0.8 06 0.6 0.4 0.2 0 Isotope 609.28081 Obs % Calc % Obs m/z Calc m/z Diff (ppm) 1 100 100 609.28081 609.28066 0.25 2 35.87 37.24 610.28425 610.28393 0.52 3 8.58 8.59 611.28650 611.28671-0.34 4 1.41 1.48 612.28987 612.28941 0.75 5 0.14 0.21 613.29210 613.29203 0.11 610.28425 611.28650 612.28987 613.29210 609 610 611 612 613 614 Counts vs. Mass-to-Charge (m/z) 250 ppb mass accuracy calibration and very accurate isotopic ratios

6540 Ultra High Definition QTOF Sensitivity Full Scan MS Mode- 1 picogram 4 x10 S/N = 319 RMS Excellent sensitivity, accuracy and resolution at 1 pg on column 3 x10 2 (M+H)+ 609.280347 Ratio m/z Diff. 2 Resolution ~ 33,000 Theor. Expt. Theor. Expt. (ppm) 100.00 100.00 609.2807 609.2803 0.51 37.24 36.36 610.2839 610.2832 1.25 8.59 8.39 611.2867 611.2878 1.77 1.48 1.45 612.2894 612.2926 5.29 1 1 610.283168 611.287790 612.292649 0 0.0 0.5 1 1.5 Counts vs. Acquisition Time (min) 0 607 608 609 610 611 612 613 614 615 616 Counts vs. Mass-to-Charge (m/z)

6540 Ultra High Definition QTOF Peak Wide Dynamic Range G2421A Tune Mix m/z 322 1 sec spectrum Dynamic Range: 4 5,000,000 100 Peak height 5,000,000 x10 6 4 3.5 3 2.5 2 1.5 322.05432 20535 = 50,000000 x10 4 324.05976 21517 35 3.5 3 2.5 2 3 x10 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 06 0.6 0.4 0.2 325.06230 21835 Height 100 326.20440 17254 325 325.5 326 326.5 Co x10 5 1.5 2.75 323.05854 2.5 20415 1 2.25 0.5 325.06230 324.32499 21835 326.20440 2 11265 17254 0 1.75 324 324.5 325 325.5 326 326.5 1.5 Counts vs. Mass-to-Charge (m/z) 125 1.25 1 0.75 324.05976 0.5 21517 0.25 323.33313 324.32499 325.06230 326.20440 16656 11265 21835 17254 0 323 323.5 324 324.5 325 325.5 326 326.5 Counts vs. Mass-to-Charge (m/z) 1 0.5 0 323.05854 20415 324.05976 325.06230 326.20440 21517 21835 17254 322 322.5 323 323.5 324 324.5 325 325.5 5 326 Counts vs. Mass-to-Charge (m/z) +ESI Scan (#27) Frag=180.0V 2GHz_accF.d

1290/6540 90 sec analysis 250 pesticides (125pg on-column) 4 x10 2.4 145.064819 24909 2.2 2 1 naphthol Mass [M+H] + = 145.064891 Resolution = 24909 0.06 ppm 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 146.068939 17868 x10 4 5.2 74.9 5 Cpd 201: Naphthol, 1-4.8 4.6 4.4 4.2 4 3.8 3.6 3.4 3.2 3 2.8 2.6 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 145 145.5 146 146.5 147 Counts vs. Mass-to-Charge (m/z) x10 4 9 8.5 8 7.5 7 6.5 6 5.5 5 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 732.468714 47094 Spinosad A Mass [M+H] + = 732.468714 Resolution = 47094 733.472265 42144 734.475147 35133 0.77ppm 731 731.5 732 732.5 733 733.5 734 734.5 735 735.5 736 736.5 737 737.5 738 Counts vs. Mass-to-Charge (m/z) 4 x10 5.5 4.5 3.5 422.208059 38636 5 Fenpyroximate E Mass [M+H] + = 422.208059 4 Resolution = 38636 3 0.25ppm 2.5 2 1.5 1 0.5 0 423.211263 33121 424.213956 29032 421.5 422 422.5 423 423.5 424 424.5 425 425.5 Counts vs. Mass-to-Charge (m/z) 58 60 62 64 66 68 70 72 74 76 78 80 82 84 86 88 90 Counts vs. Acquisition Time (sec)

Horse Heart Myoglobin (MW 16951 Da) 6540 QTOF, High Res Mode 4 21+ 20+ 19+ Average resolution of these peaks R = 38045 4 22+ 18+ 23+ 17+ 16+ Heme 24+ 15+ 25+ 14+ 13+ 26+ 12+ 11+ 10+ 22 Intact Proteins on 6540 QTOF April 2009

3 4 Chicken Lysozyme (MW 14305 Da) 6540 QTOF, High Res Mode 4 10+ R ~ 38400 11+ R ~ 36500 2 9+ R ~ 37600 12+ 14+ 13+ 8+ 23 Intact Proteins on 6540 QTOF April 2009

What is the Value of Ultra High Definition Q-TOF Coupled with 1290 Infinity UHPLC? Less ion suppression Find more trace level compounds Reduce number of false positives Higher confidence in molecular formula generation More confidence in compound ID Faster analysis greater productivity Higher peptide sequence coverage Greater sensitivity

Improving Data Quality- Faster is Better! Take advantage of peak capacity to find more trace level compounds in complex mixtures Take advantage of peak capacity to reduce ion suppression from interfering co-eluting matrix ions Take advantage of high speed acquisition to increase data g g p q sampling for better mass accuracy and mass resolution