Improve Stem Cell Yield

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Improve Stem Cell Yield Stem Cell Investigations - Total In Vitro Environment Sci-tive has been designed to mimic in-vivo conditions providing a continuous cell culture environment which eliminates cellular stress linked to variations in temperature, ph and oxidation yielding better stem cell lines. Complete Lab in a Box Reduces need for laminar air flow cabinets, stereo microscopes, CO 2 incubators Stress-Free Cell Manipulation Now Stem Cell Biologists can work under stable O 2, CO 2, ph, temperature and humidity for derivation, manipulation and culturing steps Direct-Hand Access into Workstation Using sterilised and user friendly cuff & sleeve system (Ezee Sleeve ) Imaging Ready A digital stereo microscope or real-time live cell imaging system is easily accommodated Aids Compliance with EU TCD (2004/23/EU) VOC filtration and HEPA filtration create a biologically safe working environment CE (1293) Approved (MDD 93/42/EEC) Compliant with European EMC and electrical safety tests innovation in incubation

Product Specifications External Workstation Dimensions of Unit (excluding stand) 1024mm of Unit (side to side) 1650mm 760mm (additional 150mm required behind workstation for piping) Internal Workstation Dimensions 590mm 1174mm 570mm Workstation Weight Weight approx. Interlock Dimensions 230kg 210mm 270mm 200mm The Ruskinn brand was founded in 1993 and rapidly became established as one of the world s leading suppliers and manufacturers of anaerobic and modified atmosphere workstations. Ruskinn s purpose built factory is located within the Sony Technology Centre near Pencoed in South Wales, UK. Ruskinn s range of high quality workstations is available worldwide through a global network of carefully selected distributors. Atmosphere Control Temperature Ambient + 5 C - 45 C Humidity Control Ambient - 85% O2 Control 0.0% - 20.9% CO2 Control 0.0% - 30.0% Integrated HEPA Filtration System Activated Carbon Filtration System Alarms Temperature - Visual and Audible Humidity - Visual Gas Low Pressure - Visual and Audible Standard Accessories Workstation Stand 2 Racks with Perforated Shelves to Optimise Homogeneity Direct-hand Access using Ezee Sleeve and Ezee Pleez System Removable Front with UV Filtration Screen Waste Trays Gas Tight Waste Port System PC and Software On-screen Keyboard Independent Touch Pad Mouse Windows Vista Flat Screen Monitor Imaging Camera Cronus3 Imaging Software from RI Gas Control Software USB Communications Port 7 Days Continuous Storage of Event Log Data One Touch Calibration (O2) On-Screen Fault Assistance Microscope Nikon AZ100 MZ Microscope fitted with triple nose piece and a single 2x magnification lens is supplied as standard which offers 46mm of working height. Other lenses can be purchased separately (choice is 1x or 4x magnification) Digital, Stereo Microscope Options Nikon AZ100 MZ (as shown) Leica M 205 C Olympus NEW Optional Accessories Remote Monitoring For more information contact: Ruskinn Technology Ltd Suite 3 Technium Digital, Sony Technology Centre, Pencoed CF35 5HZ, UK Tel: +44 (0) 1656 868540 Fax: +44 (0) 1656 868541 www.ruskinn.com sales@ruskinn.com Distributed by: Ref: Sci-tive 10.09

Improve Stem Cell Yield Stem Cell Investigations - Total In Vitro Environment Sci-tive has been designed to mimic in-vivo conditions providing a continuous cell culture environment which eliminates cellular stress linked to variations in temperature, ph and oxidation yielding better stem cell lines. Lab in a Box Concept Reduces need for laminar air flow cabinets and CO 2 incubators Stress-Free Cell Manipulation Now Stem Cell Biologists can work under stable O 2, CO 2, ph, temperature and humidity for all manipulations, mixing and culturing steps Direct-Hand Access into Workstation Using sterilised and user friendly cuff & sleeve system (Ezee Sleeve ) Aids Compliance with EU TCD (2004/23/EU) VOC filtration and HEPA filtration create a biologically safe working environment CE (1293) Approved (MDD 93/42/EEC) Compliant with European EMC and electrical safety tests innovation in incubation

Product Specifications External Workstation Dimensions of Unit (excluding stand) 1024mm of Unit (side to side) 1650mm 760mm (additional 150mm required behind workstation for piping) Internal Workstation Dimensions 590mm 1174mm 570mm Workstation Weight Weight approx. Interlock Dimensions 230kg 210mm 270mm 200mm Stem Cell Investigations - Total In Vitro Environment The Ruskinn brand was founded in 1993 and rapidly became established as one of the world s leading suppliers and manufacturers of anaerobic and modified atmosphere workstations. Ruskinn s purpose built factory is located within the Sony Technology Centre near Pencoed in South Wales, UK. Ruskinn s range of high quality workstations is available worldwide through a global network of carefully selected distributors. Atmosphere Control Temperature Ambient + 5 C - 45 C Humidity Control Ambient - 85% O2 Control 0.0% - 20.9% CO2 Control 0.0% - 30.0% Integrated HEPA Filtration System Activated Carbon Filtration System Standard Accessories Workstation Stand 2 Racks with Perforated Shelves to Optimise Homogeneity Direct-hand Access using Ezee Sleeve and Ezee Pleez System Removable Front with UV Filtration Screen Waste Trays Gas Tight Waste Port Gas Control Software USB Communications Port 7 Days Continuous Storage of Event Log Data One Touch Calibration (O2) On-Screen Fault Assistance Alarms Temperature - Visual and Audible Humidity - Visual Gas Low Pressure - Visual and Audible NEW Optional Accessories Remote Monitoring For more information contact: Ruskinn Technology Ltd Suite 3 Technium Digital, Sony Technology Centre, Pencoed CF35 5HZ, UK Tel: +44 (0) 1656 868540 Fax: +44 (0) 1656 868541 www.ruskinn.com sales@ruskinn.com Distributed by: Ref: Sci-tive-N 10.09

PRODUCT SPECIFICATION SUMMARY Hypoxia Workstations Model INVIVO 2 200 INVIVO 2 300 INVIVO 2 400 INVIVO 2 500 INVIVO 2 1000 SCI-TIVE Gas Mixer Q Bench Space (mm) Height 650 mm 650 mm 750 mm 750 mm 780 mm 1024 mm 405 mm Width 1100 mm 1100 mm 1420 mm 1720 mm 3000 mm 1650 mm 260 mm Depth 660 mm 660 mm 720 mm 720 mm 720 mm 760 mm 590 mm Internal Dimensions (mm) Height 420 mm 420 mm 480 mm 480 mm 480 mm 590 mm Width 500 mm 500 mm 800 mm 800 mm 800 mm 1174 mm Depth 460 mm 460 mm 500 mm 500 mm 500 mm 570 mm Maximum Capacity (90mm plates) 300 plates 270 plates 620 plates 600 plates 1200 plates Working Capacity (90mm plates) 220 plates 200 plates 470 plates 440 plates 890 plates Weight (kg) 65 kg 75 kg 100 kg 140 kg 200 kg 230 kg 10 kg Interlock Dimensions (mm) Height 200 mm 200 mm 260 mm 300 mm 300 mm 210 mm Width 100 mm 150 mm 120 mm 320 mm 320 mm 270 mm Depth 100 mm 230 mm 280 mm 300 mm 300 mm 200 mm Interlock Capacity (90mm plates) 10 plates 18 plates 39 plates 78 plates 78 plates 66 plates Interlock Cycle Time 15 sec 35 sec 45 sec 3.5 min 3.5 min Interlock Door Operation Manual Manual Manual Automated Automated Manual Activated Carbon Filtration System HEPA Filtration System Gas Mixer Q O2 Control 0.0% - 20.9% CO2 Control 0.0% - 30.0% USB Communications Port 7 Day Continuous Data Storage One Touch Calibration (O 2 ) On-Screen Fault Assistance Cycle Programming UV Filtration Screen Removable Front Internal Mains Socket Single Plate Entry System Ezee Sleeve Bare-hand Access Petri Dish Holders 3 holders O O O O O Racks 1 wire 1 wire 2 wire 2 acrylic Energy Saving Fluorescent Illumination Inspection Spotlamp Waste Trays Gas Tight Waste Port Alarms (Gas / Temperature / Humidity) Cable Gland Port O O O O O O Vacuum Line Port O O O O O O Gas Sample Port O O O O O O Automated Humidity Control Ultrasonic Humidity Control O O O O O Workstation Stand O O O O O O Palladium Catalyst O O O O O O Anaerobic Indicator Strips O O O O O O Gas Tank Regulators O O O O O O O Gas Tank Filter Modules O O O O O O O Remote Monitoring Power Failure Back Up System O O O O O O Data Logging Connection O O O O O O Fabrication: Gas Supply Solvent Bonded Acrylic Temperature Control Ambient + 5ºC to 45ºC Humidity Control Ambient to 85% Up to 4 Separate Cylinders: H 2 /N 2 ; CO 2 ; N 2 ; Air Standard O Optional Not Applicable innovation in incubation Ref: Hypoxia Specs 10.09

The Ruskinn brand was founded in 1993 and rapidly became established as one of the world s leading suppliers and manufacturers of anaerobic and modified atmosphere workstations. Ruskinn s purpose built factory is located within the Sony Technology Centre near Pencoed in South Wales, UK. Ruskinn s range of high quality workstations is available worldwide through a global network of carefully selected distributors. Ruskinn Technology Ltd Suite 3 Technium Digital Sony Technology Centre Bridgend CF35 5HZ Tel: +44 (0) 1656 868 540 Fax: +44 (0) 1656 868 541 Email: sales@ruskinn.com www.ruskinn.com Ref: Hypoxia Packet 10.09