12 x 1.8 ml. Polyethylene 250 μl x ,500 11, μl x ,500 11,500

Transcription

1 Benchtop and Microcentrifuges Genomics Cell Analysis Particle Characterization Capillary Electrophoresis Lab Automation Centrifugation Bioseparation Lab Tools BR-9742D-E

2 F x 1.8 ml F1202 r min (31 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular or ganelles, DNA, viruses, bacteria, mitochondria, chlo ro plasts or algae. 1 r av (47.5 mm) r max (64 mm) 45 Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 30,000 64, x 2.0 ml 24 ml 11 x 45 mm 0.44 x 1.8 in For use in the Allegra 64R centrifuge. No F1202 Fixed-Angle Rotor, for 30,000 rpm operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml)** Open Top Tubes, Plain Polyethylene 250 μl x ,500 11, μl x ,500 11,500 Open Top Tubes, Coated Polyethylene Heparin/Lithium/Fluoride coated 250 μl x ,500 11,500 Heparin/Lithium coated 250 μl x ,500 11,500 Heparin/Lithium/Fluoride coated 400 μl x ,500 11,500 Heparin/Lithium coated 400 μl x ,500 11,500 Tubes with Attached Caps Polyallomer x , ,000 Polyethylene x ,200 10,000 Tubes with Separate Caps Polypropylene 400 μl x ,500 11, x , ,000 Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ , μl ** Unless otherwise noted. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

3 F x 38.5 ml Order Online F0630 r min (23 mm) r av (50.5 mm) r max (78 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting sub cellu lar organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 26,200* 59, x 38.5 ml 231 ml 25.3 x 92 mm 1 x 4 in 1 30 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 21 Series centrifuges. No F0630 Fixed-Angle Rotor, for 26,200 rpm * operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polycarbonate x 89 59, ,200 Bottles with Screw Caps Polyallomer x 92 59, ,200 Polycarbonate x 92 59, ,200 Open Top Tubes Polyallomer, Thin-wall x 76 28,300 18,000 Polyallomer, Thick-wall x 89 59, ,200 Ultra-Clear x 89 59, ,200 ** In the Allegra 64R centrifuge the maximum rpm is 15,300 and the maximum g-force is 20,450. In the Allegra X22, the maximum rpm is 14,500 and the maximum g-force is 18,300. In the Allegra X-22R, This rotor is rated at a lower speed in the Allegra 21 and 21R centrifuges, so g-forces and k-factors are different. See the rotor manual for specifi c information. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Beckman Coulter Benchtop and Microcentrifugation 1-11

4 F2402H 24 x 1.8 ml F2402H 1 45 rmin (50 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 26,000 a 62, x 2.0 ml 48 ml 11 x 45 mm 0.44 x 1.8 in rav (66 mm) r max (82 mm) For use in Allegra 64R, Allegra X-22 Series and discontinued Allegra 21 Series centrifuges. No F2402H Hermetically Sealed BioSafe Rotor, for rpm * operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml)** Open Top Tubes, Plain Polyethylene 250 μl x ,900 11, μl x ,900 11,500 Open Top Tubes, Coated Polyethylene Heparin/Lithium/Fluoride coated 250 μl x ,900 11,500 Heparin/Lithium coated 250 μl x ,900 11,500 Heparin/Lithium/Fluoride coated 400 μl x ,900 11,500 Heparin/Lithium coated 400 μl x ,900 11,500 Tubes with Attached Caps Polyallomer x ,084 20,000 Polyethylene x ,960 10,000 Tubes with Separate Caps Polypropylene 400 μl x ,900 11, x , ,000 Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ , μl a* In the Allegra 64R centrifuge. In the Allegra X22, the maximum rpm is and the maximum g-force is In the Allegra X-22R, the maximum rpm is and the maximum g-force is This rotor is rated at a lower speed in the Allegra 21 and 21R centrifuges, so g-forces and k factors are different. See the rotor manual for specifi c information. ** Unless otherwise noted. Rotor Re placement Parts Rotor Lid O-ring (small) Tie-down Screw T-handle Rotor Wrench Rotor O-ring (large) Rotor Lid Adapters Beckman Coulter Benchtop and Microcentrifugation

5 F x 10 ml Order Online F1010 r min (20 mm) r av (48 mm) r max (76 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 26,000* 57, x 10 ml 100 ml 16.1 x 81.1 mm x 3 in 1 35 For use in Allegra 64R, Allegra X-22 Series and discontinued Allegra 21 Series centrifuges. No F1010 Fixed-Angle Rotor, for 26,000 rpm * operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Bottles with Screw Caps Polyallomer x , ,000 Polycarbonate x 80 57, ,000 Teflon x , ,000 Tubes Polyallomer, Thick-wall x 64 26, x 76 57, ,000 Polycarbonate x 64 26, x 76 57, ,000 Ultra-Clear x 76 57, ,000 ** In the Allegra 64R centrifuge. This rotor is rated at 15,300 rpm/19,926 x g in the Allegra X-22R and at 14,500 rpm/17,896 x g in the Allegra X-22. Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. To facilitate tube removal, place rubber pad (part number ) at the bottom of the tube cavity before inserting the tube. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Beckman Coulter Benchtop and Microcentrifugation 1-13

6 F x 1.8 ml F r min (47 mm) r av (62 mm) r max (77 mm) r min (57 mm) 45 Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Outer/Inner Outer/Inner Volume/Size 22,000 47,618/ 224/ x 2 ml 72 ml 41, x 45 mm 0.48 x 1.8 in r av (72 mm) r max (87 mm) For use in Allegra 64R and discontinued Allegra 21 Series centrifuges. 45 No F3602 Fixed-Angle Rotor, for 22,000 rpm * operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Outer/Inner Outer/Inner Speed Tube (ml)** Tubes with Attached Caps Polyallomer x ,618/41, /260 22,000 Polyethylene x ,740/8, / ,000 Tubes with Separate Caps Polypropylene μl x ,900/11, /944 11, μl x ,900/11, /944 11, x ,618/41, /260 22,000 Tubes, Plain Polyethylene μl x ,900/11, /944 11, μl x ,900/11, /944 11,500 Tubes, Coated Polyethylene μl a x ,900/11, /944 11, μl x ,900/11, /944 11, μl a x ,900/11, /944 11, μl x ,900/11, /944 11,500 Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ , μl a* In the Allegra 64R centrifuge. This rotor is rated at a lower speed in the Allegra 21 and 21R centrifuges, so g-forces and k factors are different. See the rotor manual for specifi c information. ** Unless otherwise noted Heparin-Lithium coated. * a Heparin-Lithium Fluoride coated. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Lid Screw Lid Handle Adapters Beckman Coulter Benchtop and Microcentrifugation

7 F x 50 ml Order Online F0650 r min (21 mm) r av (52.5 mm) r max (84 mm) 25 Fixed-Angle Rotor, Aluminum Major applications: Density gradient separations of erythrocytes, cell lysate fractions and granules, as well as different separations of DNA, proteins, and viruses. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 21,000 41, x 50 ml 300 ml 29 x 107 mm x 4.25 in 1 For use in the Allegra 64R centrifuge. No F0650 Fixed-Angle Rotor, for 21,000 rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polyallomer x , , x , ,000 Polycarbonate x , ,000 Bottles with Screw Caps Polyallomer x , ,000 Polycarbonate x , ,000 Teflon* with High-speed x , ,000 Screw Cap Tubes Polycarbonate x , ,000 Polycarbonate, Graduated x ,300 18,500 Polypropylene x , ,000 Tubes with Snap-On Caps Polycarbonate x , ,500 Polypropylene x , ,500 ** Teflon is a registered trademark of E. I. Du Pont de Nemours & Company. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Beckman Coulter Benchtop and Microcentrifugation 1-15

8 F x 85 ml F r min (21 mm) r av (55.5 mm) r max (90 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 20,000 40, x 85 ml 340 ml 38 x 104 mm 1.5 x 4 in 30 For use in the Allegra 64R centrifuge. No F0485 Fixed-Angle Rotor, for 20,000 rpm operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polycarbonate x , , x , ,000 Bottles with Screw Caps Polyallomer 50* x ,248 20,000 Polycarbonate x ,248 20,000 Polypropylene with Special Cap x , ,000 Tubes Polyallomer, Thick-wall x , ,000 Polyallomer, Thin-wall x , ,000 Polycarbonate x ,248 20, x , ,000 Polypropylene x ,248 20,000 ** Run with reduced fill volume to prevent spilling/leaking. To facilitate tube removal, place rubber pad (part number ) at the bottom of the tube cavity before inserting the tube. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

9 F x 50 ml Order Online F0850 r min (33 mm) r av (63.5 mm) r max (94 mm) Fixed-Angle Rotor, Aluminum Major applications: Density gradient separations of erythrocytes, cell lysate fractions and granules, as well as different separations of DNA, proteins, and viruses. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 16,500* x 50 ml 400 ml 29 x 107 mm x 4.25 in 1 25 For use in Allegra 64R, Allegra X-22 Series and discontinued Allegra 21 Series centrifuges. No F0850 Fixed-Angle Rotor, for 16,500 rpm* operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Bottles with Cap Assemblies Polyallomer x , , x , ,500 Polycarbonate x , ,500 Bottles with Screw Caps Polyallomer x , ,500 Polycarbonate x , ,500 Teflon with High-speed x , ,500 Screw Cap Tubes Polycarbonate x , ,500 Polycarbonate, Graduated x ,800 1,570 13,000 Polypropylene x , ,500 Tubes with Snap-On Caps Polycarbonate x , ,500 Polypropylene x , ,500 ** In the Allegra 64R centrifuge. This rotor is rated at 10,000 rpm/10,528 x g in the Allegra X-22R and at 9,000 rpm/8,528 x g in the Allegra X-22. Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation 1-17

10 F x 85 ml F r min (25 mm) r av (61 mm) r max (97 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 15,500* 26,320 1,428 6 x 85 ml 510 ml 38 x 104 mm 1.5 x 4 in 25 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 21 Series centrifuges. No F0685 Fixed-Angle Rotor, for 15,500 rpm* operation. Tubes and bottles not included. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Bottles with Cap Assemblies Polycarbonate x ,320 1,428 15, x ,320 1,428 15,500 Bottles with Screw Caps Polyallomer x ,320 1,428 15,500 Polycarbonate x ,320 1,428 15,500 Polypropylene x ,320 1,428 15, ** x ,320 1,428 15,500 Tubes Polyallomer, Thick-wall x ,320 1,428 15,500 Polyallomer, Thin-wall x ,320 1,428 15,500 Polycarbonate x ,320 1,428 15, x ,320 1,428 15,500 Polypropylene x ,320 1,428 15,500 ** In the Allegra 64R centrifuge. This rotor is rated at rpm/ x g in the Allegra X-22R and at rpm/6 953 x g in the Allegra X-22. Requires adapter pad ** Run with reduced fill volume to prevent spilling/leaking. To facilitate tube removal, place rubber pad (part number ) at the bottom of the tube cavity before inserting the tube. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

11 TA x 1.5 ml Order Online TA mm 83.5 mm mm 45 rmin rav rmax Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) 15,000 25, x 1.5 ml 0:34/0:50 11 x 45 mm 0.5 x 1.8 in. For use in the Allegra 25R centrifuge. 1 No TA Fixed-Angle BioSafe Rotor. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Tubes with Snap-on Caps Polyallomer Natural x ,000 Polyethylene μl x , μl x , μl x , μl x , μl x , μl x , x ,000 Polypropylene Natural x ,500 Tubes, Other Manufacturers 500- to 750-μL Tubes 500 μl/ , μl Heparin-Lithium-Fluoride coated. Heparin-Lithium coated. Rotor Replacement Parts Lid Assembly O-ring Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation 1-19

12 FX x 2.2 ml FX r min (67 mm) r av (84 mm) r max (100 mm) Fixed-Angle Rotor, Material Major applications: Pelleting, subcellular organelles, nucleic acids, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 14,000* 21, x 2.2 ml 66 ml 11 x 45 mm 0.48 x 1.8 in For use in Allegra X-22 Series centrifuges. No FX301.5 Fixed-Angle Rotor Assembly. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapter Adapter Speed Tube (ml) Tubes with Snap-on Caps Polyallomer Natural, Attached cap x ,000* Polyethylene Heparin-Lithium-Fluoride Coated μl x ,500 Heparin-Lithium Coated μl x ,500 Plain μl x ,500 Heparin-Lithium-Fluoride Coated μl x ,500 Heparin-Lithium Coated μl x ,500 Plain μl x ,500 Attached cap x ,000 Polypropylene Natural, Separate Cap x ,000* 500- to 750 μl Tubes** 500 μl/ , μl ** In refrigerated centrifuges; maximum speed in unrefrigerated centrifuges is 13,000 rpm. In refrigerated centrifuges; g-force at r max in unrefrigerated centrifuges is 19,515 g. No adapter is required with this tube; however, adapter (pkg. of 12) can be used for proper conical support at higher speeds. ** Commercially available. Rotor Re placement Parts Rotor Lid Assembly Lid O-ring Rotor O-ring Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

13 FX x 100 ml Order Online FX r min r av r max (35.0 mm) (66.5 mm) (98.0 mm) Fixed-Angle Rotor, Aluminum Major applications: Density gradient separations of erythrocytes, cell lysate fractions and granules; differential separation of DNA, proteins, and viruses. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 10,200 11,400 6 x 100 ml 1:30/1:20 38 x 102 mm 1.5 x 4 in For use in Allegra X-12 and X15R Series centrifuges. 1 No FX6100 Fixed-Angle BioSafe Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Screw Caps Polyallomer x ,400 10,200 Polycarbonate x ,400 10,200 Polypropylene x ,400 10, x ,400 10,200 Bottles with Cap Assemblies Polycarbonate x ,400 10, x ,400 10,200 Tubes with Snap-on Caps Polyallomer x ** 3 10,200 Polyethylene x ** 3 10,000 Polypropylene x ** 3 10,200 Tubes Polyallomer, Thick-wall * x ,400 10,200 Polyallomer x ,400 10,200 Polycarbonate x ,400 10, x ,400 10, x ,400 10, x ,400 10, * x ,400 10,200 Polyethylene x ,400 10,200 Polypropylene x ,400 10, x ,400 10,200 ** To facilitate tube removal, place rubber pad (part number ) at the bottom of the tube cavity before inserting the tube. ** Can be double stacked to be used inside Rotor Replacement Parts Lid Assembly Large O-ring Small O-ring Beckman Coulter Benchtop and Microcentrifugation 1-21

14 TA x 50 ml TA Fixed-Angle Rotor, Aluminum Major applications: Pelleting cells from large volumes or cell particles from tissue homogenates. Short-column methods (such as partially filled tubes) may also be used to purify large quantities of virus in a cushion gradient. Max. rpm Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) 33.0 mm 64.5 mm 96.0 mm rmin rav rmax 14,000 21,100 8 x 50 ml 0:37/0:59 29 x 104 mm 1.25 x 4 in. For use in the Allegra 25R centrifuge. No TA Fixed-Angle Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polyallomer x ,100 1,380 14, x ,100 1,380 14,000 Polypropylene x ,100 1,380 14,000 Bottles with Screw Caps Polyallomer x ,100 1,380 14,000 Polycarbonate x ,100 1,380 14,000 Teflon with High-speed x , ,500 Screw Cap Tubes Polycarbonate x ,100 1,380 14,000 Polycarbonate, Graduated x ,800 1,570 13,000 Polypropylene x ,100 1,380 14,000 Tubes with Snap-On Caps Polycarbonate x ,100 1,380 14,000 Polypropylene x ,100 1,380 14,000 Tefl on is a registered trademark of E. I. Du Pont de Nemours & Company. Rotor Replacement Parts Lid Assembly O-ring Large O-ring Tie-down Screw T-handle Rotor Wrench 1-22 Beckman Coulter Benchtop and Microcentrifugation

15 TA x 250 ml Order Online TA Fixed-Angle Rotor, Aluminum Major applications: Pelleting cells from large volumes or cell particles from tissue homogenates. Short-column methods (such as partially filled tubes) may also be used to purify large quantities of virus in a cushion gradient. Max. rpm Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) 1 35 mm rmin 10,000 15,300 6 x 250 ml 2:00/2:00 62 x 141 mm 2.5 x 5.75 in. 86 mm 137 mm rav rmax For use in the Allegra 25R centrifuge. No TA Fixed-Angle Rotor. Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assemblies Polyallomer x , x ,000 Polypropylene x ,000 Polypropylene, Wide-mouth x ,300 3,450 10,000 Polycarbonate, Wide-mouth x ,300 3,450 10,000 Bottles with Screw Caps Polyallomer x ,000 Polycarbonate x ,000 Tubes with Snap-On Caps Polycarbonate x ,000 Polypropylene x ,000 Conical Tubes Polycarbonate with Cap x ,000 Polypropylene with Cap x ,000 Open-Top Tubes Polycarbonate x ,000 Polypropylene x ,000 Rotor Replacement Parts Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation 1-23

16 C x 15 ml C r min (38 mm) r av (65.5 mm) r max (93 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting of subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 10,000* 10,400 2, x 15 ml 150 ml 17 x 120 mm 0.65 x 4.75 in 25 For use in Allegra 64R, Allegra X-22 Series and discontinued Allegra 21 Series centrifuges. No C1015 Fixed-Angle Rotor, for 10,000 rpm* operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Tubes Conical x ,400 2,270 10,000 ** In the Allegra 64R centrifuge. This rotor is rated at 9,500 rpm/9,299 x g in the Allegra X-22R and at 9,000 rpm/8,346 x g in the Allegra X-22. Tubes available from scientifi c supply ven dors. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench 1-24 Beckman Coulter Benchtop and Microcentrifugation

17 C x 50 ml Order Online C0650 r min (32 mm) r av (62 mm) r max (92 mm) Fixed-Angle Rotor, Aluminum Major applications: General pelleting of cells, bacteria and food products; separation of proteins, viruses and subcellular fractions; phase separation; and binding studies. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 10,000* 10,400 2,680 6 x 50 ml 300 ml 28.5 x 107 mm x 4.25 in 1 25 For use in Allegra 64R, Allegra X-22 Series, and discontinued Allegra 21 Series centrifuges. No C0650 Fixed-Angle Rotor, for 10,000 rpm* operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force* k Factor Maximum Volume per (mm) Adapters Adapter Speed* Tube (ml) Tubes Conical x ,400 2,680 10,000 ** In the Allegra 64R centrifuge. This rotor is rated at 9,500 rpm/9,420 x g in the Allegra X-22R and at 9,000 rpm/8,420 x g in the Allegra X-22. Tubes available from scientifi c supply vendors. Rotor Re placement Parts Rotor Lid Rotor Lid O-ring Tie-down Screw T-handle Rotor Wrench Beckman Coulter Benchtop and Microcentrifugation 1-25

18 SX x 2.2 ml SX Swinging-Bucket Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 14,000* 16, x 2.2 ml 52.8 ml 11 x 45 mm 0.44 x 1.8 in. For use in Allegra X-22 Series centrifuges. No SX241.5 Swinging-Bucket BioSafe Rotor Assembly. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Polyallomer Natural, Attached cap x ,000* Polyethylene Heparin-Lithium-Fluoride Coated μl x ,500 Heparin-Lithium Coated μl x ,500 Plain μl x ,500 Heparin-Lithium-Fluoride Coated μl x ,500 Heparin-Lithium Coated μl x ,500 Plain μl x ,500 Attached cap x ,000 Polypropylene Natural, Separate Cap x ,000* 500- to 750-μL Tubes μl/ , μl ** In refrigerated centrifuges; maximum speed in unrefrigerated centrifuges is rpm. In refrigerated centrifuges; g-force at r max in unrefrigerated centrifuges is g. No adapter is required with this tube; however, adapter (pkg. of 12) can be used. ** Commercially available. Unless otherwise noted Rotor Re placement Parts Rotor Lid Assembly O-ring Bucket (set of 2) Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

19 H x 1.8 ml Order Online H6002 r min (35 mm) r av (64.5 mm) r max (94 mm) Horizontal (Bowl) Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 12,200* 12,400 1, x 1.8 ml 108 ml 11 x 39 mm 0.44 x 1.5 in 1 90 For use in Allegra 64R and discontinued Allegra 21 Series centrifuges. No H6002 Horizontal BioSafe Rotor, for 12,200 rpm* operation. Tubes and bottles not included. Tubes Tube Style/Material Nominal Part No. Quantity Size Tube Holder Tubes per g-force k Factor Maximum Volume per (mm) Color & Part No. Holder Speed Tube (ml) Set of 2/Set of 6 Tubes with Attached Caps Polyallomer x 38 blue / ,400 12,200 Polyethylene x 39 blue / ,320 10,000 Tubes Microtainer** 600 μl red ,300 12,200 red Polypropylene 400 μl x 40 dark green ,600 12,200 dark green μl x 28 red ,950 12,200 red x 38 blue / ,400 12,200 Plain Tubes Polyethylene 250 μl x 45 dark green ,895 11,500 dark green μl x 40 dark green ,820 10,000 dark green Coated Tubes Polyethylene Yellow 250 μl x 45 dark green ,900 11,500 dark green Polyethylene Blue 250 μl x 45 dark green ,900 11,500 dark green Polyethylene Yellow 400 μl x 40 dark green ,300 11,500 dark green Polyethylene Blue 400 μl x 40 dark green ,300 11,500 dark green ** In the Allegra 64R centrifuge. This rotor is rated at a lower speed in the Allegra 21 and 21R centrifuges, so g-forces and k factors are different. See the rotor manual for specifi c information. Bold numbers feature 30 resting angle to minimize remixing of loose pellets upon completion of run. Based on empirical tests of tube strength with the lid in place on the rotor. ** Microtainer is a registered trademark of Becton, Dickinson and Company; available commercially. Unless otherwise noted. Rotor Re placement Parts Rotor Lid Rotor Lid Knob Rotor Lid O-ring Rotor O-ring Tie-down Screw T-handle Rotor Wrench Beckman Coulter Benchtop and Microcentrifugation 1-27

20 S x Microplates S r min mm r av mm r max mm Swinging-Bucket Rotor, Aluminum Major applications: DNA sample preparation in microplates, deep-well plates, 96-well filtration kits and PCR plates. Max. rpm Max. g Number of Plates Approximate Volume/Size Accel/Decel Time (min:sec) 5,700 6, standard plates 0:47/0:20 per rotor For use in the Allegra 25R centrifuge. No S5700 Swinging-Bucket Rotor. Microplates No. of Wells Description Part No. Quantity g-force Maximum Speed 96 Multiwell Polystyrene plate pkg ,130 5, Deep-well (1-mL/well) Polystyrene Titer Plates, nonsterile /ctn. 6,130 5, Deep-well (1-mL/well) Polystyrene Titer Plates, sterile /ctn. 6,130 5, Deep-well (1-mL/well) Polypropylene Titer Plates, nonsterile /ctn. 6,130 5, Deep-well (1-mL/well) Polypropylene Titer Plates, sterile /ctn. 6,130 5, Square-well Polypropylene Titer Plates /ctn. 6,130 5,700 Rotor Re placement Parts S5700 Buckets (set of 2) S5700 Microplate Carrier (set of 2) Tie-down Screw T-handle Rotor Wrench Support Pad for Microplates (set of 4) Rubber Roller, 4 in., for Sealing Foil Microplate Lids 1-28 Beckman Coulter Benchtop and Microcentrifugation

21 SX x Microplates Order Online SX5700 Swinging-Bucket Rotor, Aluminum Major applications: DNA sample preparation in microplates, deep-well plates, 96-well filtration kits and PCR plates. Max. rpm Max. g Number of Plates Approximate Volume/Size Accel/Decel Time (min:sec) 5,700 5, standard plates or 0:33/0:25 2 deep-well plate per rotor 1 For use in the Allegra 25R centrifuge. No. A SX5700 Swinging-Bucket Rotor. Microplates No. of Wells Description Part No. Quantity g-force Maximum Speed 96 Multiwell Polystyrene plate pkg ,130 5, Deep-well (1-mL/well) Polystyrene Titer Plates, nonsterile /ctn. 6,130 5, Deep-well (1-mL/well) Polystyrene Titer Plates, sterile /ctn. 6,130 5, Deep-well (1-mL/well) Polypropylene Titer Plates, nonsterile /ctn. 6,130 5, Deep-well (1-mL/well) Polypropylene Titer Plates, sterile /ctn. 6,130 5, Square-well Polypropylene Titer Plates /ctn. 6,130 5,700 Rotor Re placement Parts A49597 SX5700 Buckets (includes Microplate Holder) (set of 2) A49599 SX5700 Microplate Holder (set of 2) A49598 Cover for Bucket Tie-down Screw T-handle Rotor Wrench Bucket, including Microplate Holder Rubber Roller, 4 in., for Sealing Foil Microplate Lids Rotor Cleaning Kit Beckman Solution 555 (1 qt) Rotor Cleaning Brush Anti-Seize Paint-on Graphite Lubricant (1/2 oz) Spinkote Lubricant (2 oz) Beckman Coulter Benchtop and Microcentrifugation 1-29

22 TS x 500 ml TS mm mm rm Swinging Bucket Rotor, Aluminum Major applications: Sedimenting protein precipitates, large particles, cells and cell debris. Max. rpm Max. g Rotor Approximate Capacity Accel/Decel Time (min:sec) 5,100 5,540 4 x 500 ml 0:59/0:47 85 x 135 mm 3.5 x 5.5 in mm For use in the Allegra 25R centrifuge. No TS Swinging-Bucket Biosafety Rotor.* Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml) Bottles with Cap Assembly Polycarbonate x ,100 Polypropylene x ,100 Bottles with Screw Caps Polyallomer x ,100 Polycarbonate x , x 135 5,540 5,100 Polypropylene x 135 5,540 5,100 Tubes with Caps Polyallomer x ,100 Open-Top Tubes Polyallomer, Thick-wall x ,100 Polycarbonate x , x ,100 ** With sealed bucket covers. Rotor Re placement Parts Buckets (set of 2) Bucket Cover (set of 2) Bucket O-ring (set of 8) Microplate Carrier (set of 2) Tie-down Screw T-handle Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

23 SX x 250 ml Order Online 71 mm 101 mm 172 mm rmin rav rmax Swinging-Bucket Rotor, Aluminum Major applications: Rapidly sediments protein precipitates, large particles, cells and cell debris. Can also be used for binding studies and for separating serum from whole blood. Max. rpm Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 4,500* 3,901 11,070 4 x 250 ml 1000 ml 61.8 x 125 mm 2.5 x 5 in 1 For use in Allegra X-22 Series centrifuges. No SX4250 Swinging-Bucket Rotor. Unshielded, four-place rotor with aluminum rotor yoke and removable aluminum swinging buckets, all anodized black for corrosion protection. Includes four buckets without covers. Bottles and Tubes Tube Style Nominal Size Required Adapter Tubes per Maximum Number Volume per (mm) (set of 2) Adapter of Tubes per Tube (ml) Rotor Microfuge/PCR Tubes x Hemolyse or RIA Tubes 1.5/ x x x Vacutainer 5 13 x Glass 7 12 x Vacutainer x Monovettes Round-Bottom x /15 17 x Conical x Glass with Open-Top or Screw-on Cap x Round-Bottom x Conical x Glass x / x ml Oakridge x Flat-bottom with Screw-on Cap x x x ** In refrigerated centrifuges; maximum speed in unrefrigerated centrifuges is 4,200 rpm. In refrigerated centrifuges; g-force at r max in unrefrigerated centrifuges is 3,398 x g. Replacement Parts Bucket Covers (set of 4) Bucket with Cover (set of 2) Bucket O-ring (set of 4) Tie-down Screw T-handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation 1-31

24 SX4750/4750A 4 x 750 ml SX4750/4750A 1 r min r av r max (207.8 mm) Swinging-Bucket Rotor, Stainless Steel/Aluminum Major applications: Rapid sedimentation of protein precipitates, large particles, cells and cell debris. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 4,750 5,250 4 x 750 ml 0:80/0:80 96 x 130 mm 3.85 x 5.25 in r min r av r max (183.2 mm) For use in Allegra X-15R and Allegra X-12 Series centrifuges. No SX4750 Swinging-Bucket BioSafe* Rotor. Four-place rotor with stainless-steel rotor yoke and removable aluminum swinging buckets. Buckets are interchangeable with SX4750 BioSafe Microplate Carriers for spinning microtiter plates or microfuge tubes using rack inserts. BioSafe Bucket Covers No Set of two. Transparent bucket covers for SX4750 round rotor buckets to contain broken tubes. Aerosolve Cannisters No Set of four. No Set of two. Aerosolve Cannisters fit in SX4750 round rotor buckets. These Biosafety cannisters feature an O-ring seal and are completely transparent so a broken tube can be seen and proper precautions taken before you break the seal. Cannisters can also be used as 500 ml wide-mouth bottles. Specially designed adapters accommodate most popular tubes within the cannister. In the Tubes and Bottles chart following, adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Cell Culture Flask Adapters No Accommodate Corning 75 cm 2 Canted-Neck Cell Culture Flasks (PN ). One flask per adapter. 3,024 rpm; 2,080 x g. No Accommodate Corning 25 cm 2 Canted-Neck Cell Culture Flasks (PN ). Two flasks per adapter. 3,200 rpm; 2,000 x g. No SX4750A Swinging-Bucket BioSafe Rotor. Four-place rotor with stainless-steel rotor yoke and removable aluminum swinging buckets. ARIES technology provides imbalance correction for rotors with buckets that are unbalanced up to 50 grams in opposing loads. SX4750μ Biosafety Microplate Carriers No SX4750μ Biosafety Microplate Carrier Assembly. Includes carriage and rubber pad to cushion plates. Each carrier can hold up to 4 microplates, 1 deep-well/square well plate, 1 rack of MiniTubes, or other labware in 96-well format. Set of 2. Maximum allowable speed in Allegra X-12 is 3,750 rpm (2,890 x g), in Allegra X-15R, maximum allowable speed is 4,450 rpm (4,060 x g). Replacement Parts for SX4750/SX4750A Bucket (set of 2) Bucket covers (set of 2) Replacement bucket O-ring (pkg/8) Bucket cover latch assembly Replacement Parts for SX4750μ Microplate Carriers Biocertified cover for SX4750μ microplate carriers Replacement cover gasket (set of 4) Replacement carriage, includes rubber pad (set of 2) Replacement rubber pad for carriage (set of 4) ** With sealed bucket covers. Rated at 3,750 rpm/3,270 x g in the Allegra X-12 Series centrifuges. Corning is a trademark of Corning Incorporated Beckman Coulter Benchtop and Microcentrifugation

25 SX4750/4750A (cont d) 4 x 750 ml Order Online SX4750/4750A 1 Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Modular Tubes per g-force* k Factor Maximum Volume per (mm) Disk Adapters Adapter Speed* Tube (ml) Set of 2/Set of 4 Bottles, Wide-mouth Polycarbonate x ,250 4,750 Polypropylene x ,250 4,750 Polypropylene x ,250 4,750 Bottles with Cap Assemblies Polyallomer x / ,250 4, / x / ,250 4, / Polycarbonate x / ,250 4, / Polycarbonate, Wide-mouth x Polypropylene, Wide-mouth x ,250 4, x ,250 4,750 Bottles with Screw Caps Polyallomer x / ,250 4, / Polycarbonate x / ,250 4, / x ,250 4, x ,250 4,750 Polypropylene x ,250 4,750 Polypropylene, Wide-mouth x ,250 4,750 Teflon with High-Speed Cap x / ,250 4, / ** Rated at 3,750 rpm/3 270 x g Allegra X-12 Series centrifuges. Adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Adapters Beckman Coulter Benchtop and Microcentrifugation 1-33

26 SX4750/4750A (cont d) 4 x 750 ml SX4750/4750A 1 Bottles and Tubes Tube Style/Material Nominal Part No. Quantity Size Required Modular Tubes per g-force* k Factor Maximum Volume per (mm) Disk Adapters Adapter Speed* Tube (ml) Set of 2/Set of 4 Open-Top Tubes Polyallomer, Thick-wall x / ,250 4, / Polyethylene x / ,250 4,750 Polycarbonate x / ,250 4, / x / ,250 4, x / ,250 4, / Polycarbonate, Graduated x / ,250 4, / Polypropylene x / ,250 4, x / ,250 4, / Tubes with Snap-On Caps Polyallomer x / ,250 4, / 24 Polycarbonate x / ,250 4, / Polycarbonate, Conical x / ** 1 5,250 4, / Polyethylene x / ,250 4, / 24 Polypropylene, Conical x / ** 1 5,250 4, / ** Rated at rpm/3 270 x g in the Allegra X-12 Series centrifuges. Adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Requires 1.5-mL adapter plate PN ** Requires adapter PN , available 1 each pack. Adapters Beckman Coulter Benchtop and Microcentrifugation

27 SX4750/4750A (cont d) 4 x 750 ml Order Online SX4750/4750A 1 Tubes with Snap-On Caps (cont d) Tube Style/Material Nominal Part No. Quantity Size Required Modular Tubes per g-force* k Factor Maximum Volume per (mm) Disk Adapters Adapter Speed* Tube (ml) Set of 2/Set of 4 Polypropylene x / ,250 4, / Orange x / ,250 4, / Yellow x / ,250 4, / Green x / ,250 4, / Blue x / ,250 4, / Natural x / ,250 4, / Natural** x / ,250 4, / BioVials Polypropylene , x / ,250 4, / Blood-Bag Cups No Yellow cup with inner diameter of 90 mm for singleor double-packs. No Orange cup with inner diameter of 97 mm for tripleor quad-packs. SX4750μ Biosafety Microplate Carriers No SX4750 BioSafe Microplate Carrier Assembly. Includes carriage and rubber pad to cushion plates. Each carrier can hold up to 4 microplates, 1 deep-well/square well plate, 1 rack of MiniTubes, or other labware in 96-well format. Set of 2. Maximum allowable speed is rpm (2 890 x g). ** Rated at 3,750 rpm/3,270 x g in the Allegra X-12 Series centrifuges, rpm/4 060 x g in Allegra X-15R. Adapters required for the use of tubes within Aerosolve Cannisters are listed in bold type. Requires 1.5-mL adapter plate PN , sold individually. Rotor Supplies Round Bucket (set of 2) Round Bucket Cover (set of 2) Round Bucket O-ring (pkg of 8) Round Bucket Cover Latch Assembly Bucket Cover Air-Vent Filter (pkg of 60) Adapters Beckman Coulter Benchtop and Microcentrifugation 1-35

28 S x Microtiter Plates S rmin (70 mm) rav rmax (110 mm) Swinging-Bucket Rotor (Unshielded), Anodized Aluminum Major applications: Serial dilution of small liquid volumes. Max. rpm Max. g Number of Plates Approximate Volume/Size Accel/Decel Time (min:sec) 3,000 1,107 2 x 96 wells 0:21/0:20 For use in Allegra X-22 Series and discontinued Allegra 21 Series centrifuges. No S2096 Microtiter Rotor Assembly, for 3,000 rpm operation. Unshielded, two-place rotor, designed to accommo date 96-well and deep-well microplates. Racks are available that accommodate 1 ml MiniTubes. Microplates No. of Wells Description Part No. Quantity g-force Maximum Speed 96 Microplates pkg ,110 3, Deep-well (1-mL/well) Polystyrene Titer Plates, nonsterile /ctn. 1,110 3, Deep-well (1-mL/well) Polystyrene Titer Plates, sterile /ctn. 1,110 3, Deep-well (1-mL/well) Polypropylene Titer Plates, nonsterile /ctn. 1,110 3, Deep-well (1-mL/well) Polypropylene Titer Plates, sterile /ctn. 1,110 3, Square-well Polypropylene Titer Plates /ctn. 1,110 3,000 Accessories for Microplates No Caps for 96-well Deep-well Titer Plates, Nonsterile (10/ctn) No Caps for 96-well Titer Plates, Sterile (10/ctn) MiniTube Racks, Tubes, and Accessories No MiniTube Rack No MiniTube Test Tubes (1-mL), Polystyrene, Nonsterile (400 strips of 12) No MiniTube Test Tubes (1-mL), Polypropylene, Nonsterile (400 strips of 12) No Caps for MiniTubes, Polyethylene, Nonsterile (80 strips of 12) No Caps for MiniTubes, Polyethylene, Sterile (80 strips of 12) Rotor Re placement Parts Tie-down Screw T-handle Rotor Wrench Tray (set of 2) 1-36 Beckman Coulter Benchtop and Microcentrifugation

29 Microcentrifugation 2 Microfuge 16 Microcentrifuge Microfuge 18 Microcentrifuge Microfuge 22R Microcentrifuge Microfuge Microcentrifuges Rotor Summary Fixed-Angle Rotors F F241.5P F F F12x FX121.5P FX241.5P Swinging-Bucket Rotor S Beckman Coulter Benchtop and Microcentrifugation 2-1

30 Microcentrifugation Microfuge 16 Microcentrifuge 2 The Microfuge 16 is fast and powerful for optimal pelleting on the bench or in the cold room. Perfect for DNA, RNA, proteins, and virus cell isolation. Its maintenance-free motor spins up to 14,800 rpm (16,163 x g). Easy-to-read interface displays both speed (rpm) and force (rcf). Comes complete with a 24-place, aerosol-tight, autoclavable, fixed-angle rotor. A convenient snap-on rotor lid (not aerosol tight) is included as an accessory to the rotor. Select timed, hold, or pulse (short) run times. Microfuge 16 Microcentrifuge Part Numbers A /60 Hz, 100/120 V, with FX241.5P rotor A /60 Hz, 220/240 V, with FX241.5P rotor A /60 Hz, 100/120 V A /60 Hz, 220/240 V Specifications Maximum Speed Maximum g-force Maximum capacity Drive Type Time Setting Accel/Decel Rates* Power Consumption Maximum Noise Output Dimensions Weight 14,800 rpm 16,163 x g 24 x 2.2 ml Brushless 0 99 minutes, 59 seconds, continuous, pulse (short run) 15 sec accel/13 sec decel to/from max speed 95 W 60 dba 17.6 cm (6.9 in) H x 26.6 cm (10.5 in) D x 22.6 cm (8.9 in) W 6.4 kg (14 lb) 2-2 Beckman Coulter Benchtop and Microcentrifugation

31 Microcentrifugation Order Online Microfuge 18 Microcentrifuge The Microfuge 18 is fast and powerful for optimal pelleting on the bench or in the cold room. Perfect for DNA, RNA, proteins and virus cell isolation. Its maintenance-free motor spins up to 14,000 rpm (18,000 x g). Easy-to-read interface displays both speed (rpm) and force (rcf). Comes complete with a 24-place, aerosol-tight, autoclavable, fixed-angle rotor. A convenient snap-on rotor lid is available as an accessory to the rotor. Select timed, hold, or pulse (short) run times. 2 Specifications Microfuge 18 Microcentrifuge Part Numbers /60 Hz, 100/120 V, with F241.5P rotor Hz, 230 V, with F241.5P rotor Maximum Speed 14,000 rpm Maximum g-force 18,000 x g Drive Type Brushless Time Setting 0 30 minutes, continuous, pulse (short run) Accel/Decel Rates 18 sec accel/19 sec decel to/from max speed Maximum Heat Output 170 Btu/h (50 W) Maximum Noise Output 58 dba Dimensions 24.0 cm (9.5 in) H x 32.0 cm (12.6 in) D x 24.5 cm (9.7 in) W Weight 13.0 kg (28.7 lb) Beckman Coulter Benchtop and Microcentrifugation 2-3

32 Microcentrifugation Microfuge 22R Microcentrifuge 2 The Microfuge 22R offers high g -force ( x g) for optimal pelleting. Its maintenance-free motor spins up to rpm and maintains 4 C at maximum speed to protect precious samples. Perfect for DNA, RNA, proteins and virus cell isolation. Available with six interchangeable rotors, all of which feature aerosol-tight lids to minimize contamination from broken or leaking tubes. Convenient snap-on rotor lids are available as accessories to the F241.5, F241.5P and F40.25 rotors. All rotors are also autoclavable at 121 C. Easy-to-use control knobs and easy-to-read display. Specifications Microfuge 22R Microcentrifuge Part Numbers Hz, 120 V Hz, 230 V /60 Hz, 100 V Hz, 120 V with F241.5P rotor Hz, 230 V with F241.5P rotor /60 Hz, 100 V with F241.5P rotor A Hz, 120 V with F301.5 rotor A Hz, 230 V with F301.5 rotor A /60 Hz, 100 V with F301.5 rotor Maximum Speed rpm Maximum g-force x g Drive Type Brushless induction Time Setting 0 30 minutes, continuous, pulse (short run) Accel/Decel Rates 18 sec accel/19 sec decel to/from max speed Maximum Heat Output Btu/h (630 W) Maximum Noise Output 58 dba Dimensions 36.5 cm (14.4 in) H x 58.5 cm (23.0 in) D x 30.0 cm (11.8 in) W Weight 41.0 kg (90.4 lb) 2-4 Beckman Coulter Benchtop and Microcentrifugation

33 Microcentrifugation Order Online Microfuge Centrifuges Rotor Summary Rotor Part Maximum Maximum Number Tubes/Bottles Rotor Approx. Rotor BioSafe Type Number Speed Force (g) and Size Capacity Accel. Angle (rpm) at r max (diameter x length) (ml) Time* mm / in. (min:sec) Fixed-Angle Rotors F ,300 20, x :19 45 Yes 11 x x 1.5 Pellet subcellular organelles, DNA, viruses, bacteria, or chloroplasts. Microfuge 16 Microfuge 18 Microfuge 22R 2 F241.5P ,000 17, x :18 45 No 11 x x 1.5 Pellet subcellular organelles, nucleic acids, viruses, bacteria, or chloroplasts. F ,000 21, x :40 45 Yes 11 x x 1.8 Pellet subcellular organelles, viruses, bacteria, chloroplasts, mitochondria, or algae. F ,000 17, x 0.40 ml :19 30 Yes 7 x x 1.5 Pellet subcellular organelles, DNA, viruses, bacteria, or chloroplasts. F12x ,000 20, x 8 (200 μl) :38 45 No PCR strip Minimize post-pcr condensation, post-reaction cleanup. FX121.5P A ,800 15, x 1.5/ :13 45 No 11 x x 1.6 Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts, or algae. FX241.5P A ,800 16, x 1.5/ :15 32 /37 No 11 x x 1.6 Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts, or algae. Swinging-Bucket Rotor S ,000 16, x :30 90 No 11 x x 1.8 Pellet subcellular organelles, viruses, bacteria, chloroplasts, mitochondria, or algae. ** To maximum speed, rotor fully loaded. Beckman Coulter Benchtop and Microcentrifugation 2-5

34 F241.5 F x 2.2 ml 2 No F241.5 BioSafe Rotor Assembly. Transparent high-impact plastic lid. Tubes 45 r min (50.3 mm) r av (67.6 mm) r max (82.8 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, DNA, viruses, bacteria or chloroplasts. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 15,300 20, x 2.0 ml 0:19/0:23 11 x 39 mm 0.44 x 1.5 in For use in Microfuge 22R Microcentrifuge. Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapter Adapter Speed Tube (ml)* Polyallomer Natural, Attached cap x , ,300 Polyethylene Heparin-Lithium-Fluoride Coated 250 μl x ,500 Heparin-Lithium Coated 250 μl x ,500 Plain 250 μl x ,500 Heparin-Lithium-Fluoride Coated 400 μl x ,500 Heparin-Lithium Coated 400 μl x ,500 Plain 400 μl x ,500 Attached cap x ,960 1,190 10,000 Polypropylene Natural, Separate Cap μl x , x , ,300 ** Unless otherwise noted Rotor Re placement Parts Convenient Snap-on Lid Rotor Lid Assembly Rotor Lid Knob O-ring T ie-down Screw T -handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

35 F241.5P F241.5P 24 x 1.8 ml Order Online 45 r min (50.2 mm) Fixed-Angle Rotor, High-Impact Thermoplastic Major applications: Pelleting subcellular organelles, nucleic acids, viruses, bacteria or chloroplasts. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,000 17, x 2.0 ml 0:18/0:19 11 x 39 mm 0.44 x 1.5 in r av (67.4 mm) r max (82.5 mm) For use in Microfuge 18 and 22R Microcentrifuge. No F241.5P Rotor Assembly. Rotor is made of high-impact thermoplastic and snap-on lid is polycarbonate. 2 Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml)* Polyallomer Natural x 38 17, ,000 Polyethylene 250 μl x , μl x , μl x , μl x , μl x , μl x , x 39 8, ,000 Polypropylene Natural 400 μl x , x 38 17, ,000 ** Unless otherwise noted Rotor Re placement Parts Convenient Snap-on Lid Aeroseal-tight Lid T ie-down Screw T -handle Rotor Wrench Tie-down Screw for Snap-on Cap Rubber Seal, Lid Adapters Beckman Coulter Benchtop and Microcentrifugation 2-7

36 F301.5 F x 2.2 ml 2 No F301.5 BioSafe Rotor Assembly. Tubes 45 r min (67 mm) r av (84 mm) r max (100 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,000 21, x 2.2 ml 0:40/0:38 11 x 45 mm 0.44 x 1.8 in. For use in Microfuge 22R Microcentrifuge. Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapter Adapter Speed Tube (ml)** Polyallomer Natural, Attached cap x * 1 14,000 Polyethylene Heparin-Lithium-Fluoride Coated 250 μl x ,500 Heparin-Lithium Coated 250 μl x ,500 Plain 250 μl x ,500 Heparin-Lithium-Fluoride Coated 400 μl x ,500 Heparin-Lithium Coated 400 μl x ,500 Plain 400 μl x ,500 Attached cap x * 1 10,000 Polypropylene Natural, Separate Cap x * 1 14, to 750-μL Tubes 500 μl/ , μl ** No adapter is required with this tube; however, adapter (pkg. of 12) can be used. Commercially available. ** Unless otherwise noted Rotor Re placement Parts Rotor Lid Assembly Lid O-ring Rotor O-ring T ie-down Screw T -handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation

37 F40.25 F x 0.25/0.40 ml No F40.25 BioSafe Rotor Assembly. Tubes 30 r min (55.7 mm) rav (68.4 mm) rmax (80.0 mm) Fixed-Angle Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,000 17, x 0.40 ml 0:40/0:38 7 x 40 mm 0.25 x 1.5 in. For use in Microfuge 22R microcentrifuge. Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (μl) Polyethylene x , x , x , x , x , x ,500 2 Rotor Re placement Parts Convenient Snap-on Lid Rotor Lid Assembly Lid O-ring Rotor O-ring T ie-down Screw T -handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation 2-9

38 F12x8.2 F12x x 8 Strips of 200 μl tubes 45 rmax (inner row) Fixed-Angle Rotor, Aluminum Major applications: Minimizing post-pcr * condensation, post-reaction clean-up. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,000 20,800 (outer row) 12 x 8 Strips 0:38/0:35 19,590 (inner row) 200 μl 2 No F12x8.2 Rotor Assembly. Tubes rmax (outer row) For use in Microfuge 22R microcentrifuge. Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (μl) PCR Tubes ,000 ** PCR is covered by patents owned by F. Hoffman-La Roche, Inc. Commercially available. Rotor Re placement Parts Rotor Lid Assembly Rotor O-ring Lid O-ring T ie-down Screw T -handle Rotor Wrench 2-10 Beckman Coulter Benchtop and Microcentrifugation

39 FX121.5P FX121.5P 12 x 1.5/2.2 ml Order Online r max (62 mm) r av (45.5 mm) No. A FX121.5P Rotor Assembly. Rotor is made of high-impact thermoplastic and snap-on lid is polysulfone. 45 r min (29 mm) Fixed-Angle Rotor, High-Impact Thermoplastic Major applications: Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts or algae. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,800 15, x 1.5/2.2 ml 0:13/0:11 11 x 40 mm 0.44 x 1.6 in For use in Microfuge 16 Microcentrifuge. 2 Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml)** Polyallomer Natural x 40 15,183 14,800 Polyethylene 250 μl x , μl x , μl x , μl x , μl x , μl x , x 39 8,960 1,190 10,000 Polypropylene x 40 15,183 14,800 PCR* Tubes 200 μl , /600.0/750 μl ,800 ** PCR is covered by patents owned by F. Hoffman-La Roche, Inc. ** Unless otherwise noted. Commercially available. Rotor Re placement Parts A46477 Convenient Polysulfone Snap-on Lid Adapters Beckman Coulter Benchtop and Microcentrifugation 2-11

40 FX241.5P FX241.5P 24 x 1.5/2.2 ml 2 Inner Row Dimensions r max (62 mm) r av (57 mm) r max (66 mm) Outer Row Dimensions r av (45.5 mm) 37 (outer row) r min (38 mm) 32 (inner row) r min (38 mm) No. A FX241.5P Rotor Assembly. Rotor is made of high-impact thermoplastic and snap-on lid is polysulfone. Fixed-Angle Rotor, High-Impact Thermoplastic Major applications: Pelleting subcellular organelles, mitochondria, viruses, bacteria, chloroplasts or algae. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,800 16, x 1.5/2.2 ml 0:15/0:13 11 x 40 mm 0.44 x 1.6 in For use in Microfuge 16 Microcentrifuge. Tubes Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml)** Polyallomer Natural x ,800 Polyethylene μl x , μl x , μl x , μl x , μl x , μl x , x ,190 10,000 Polypropylene x ,800 PCR* Tubes μl , /600.0/750.0 μl ,800 ** PCR is covered by patents owned by F. Hoffman-La Roche, Inc. ** Unless otherwise noted. Commercially available. Rotor Re placement Parts A46477 Convenient Polysulfone Snap-on Lid Adapters Beckman Coulter Benchtop and Microcentrifugation

41 S241.5 S x 2.2 ml No S241.5 Rotor Assembly. Tubes and Bottles rmin (35 mm) rav (54 mm) rmax (74 mm) Swinging-Bucket Rotor, Aluminum Major applications: Pelleting subcellular organelles, viruses, bacteria, mitochondria, chloroplasts or algae. Max. rpm Max. g Number of Tubes Approximate Volume/Size Accel/Decel Time (min:sec) 14,000 16, x 2.2 ml 0:30/0:33 11 x 45 mm 0.44 x 1.8 in. For use in the Microfuge 22R Microcentrifuge. Tube Style/Material Nominal Part No. Quantity Size Required Tubes per g-force k Factor Maximum Volume per (mm) Adapters Adapter Speed Tube (ml)* Polyallomer Natural x ,000 Polyethylene μl x , μl x , μl x , μl x , μl x , μl x , x ,000 Polypropylene Natural x , to 750-μL Tubes μl/ , μl 2 ** Unless otherwise noted. Rotor Re placement Parts Rotor Lid Assembly O-ring Bucket (set of 2) T ie-down Screw T -handle Rotor Wrench Adapters Beckman Coulter Benchtop and Microcentrifugation 2-13

42 Air-Driven Micro-Ultracentrifugation Airfuge Air-Driven Micro-Ultracentrifuge Airfuge Micro-Ultracentrifuge Rotor Summary Fixed-Angle Rotors A A-100/ A-100/ A ACR-90 Chylomicron Rotor Batch Rotor EM-90 Electron Microscopy Particle-Counting Rotor Beckman Coulter Benchtop and Microcentrifugation 3-1

43 Ultracentrifugation Airfuge Air-Driven Micro-Ultracentrifuge The unique Airfuge from Beckman Coulter is a miniature, air-driven ultracentrifuge recognized worldwide as a convenient, easy-to-use pelleting tool in research, clinical, and industrial labo ra to ries. Efficient and simple to operate, the Airfuge has a line of general-purpose and special purpose rotors for a wide variety of small-volume applications. Specifications 3 Airfuge Air-Driven Micro-Ultracentrifuge with digital tachometer Part Numbers Airfuge Hz, 120 VAC Hz, 220 VAC Hz, 120 VAC with Digital Thermometer Hz, 220 VAC with Digital Thermometer Maximum Speed Maximum g-force (RCF x g) Speed Control Imbalance Tolerance Drive Cooling Time Setting Accel/Decel Rates Heat Output Dimensions Weight Voltage Requirements 110,000 rpm 199,000 x g (A 110 Rotor) ± rpm of set speed Up to 10% of volume in opposite tubes Air-cooled minutes in five minute increments, HOLD for longer runs seconds/ minutes 0.6 kw/h (1 800 Btu/h) 28.0 cm (11 in) H x 38.0 cm (15 in) D x 20.0 cm (8 in) W 10.4 kg (23 lb) 60/50 Hz, 100 VAC 50 Hz, VAC 60 Hz, 110 VAC 3-2 Beckman Coulter Benchtop and Microcentrifugation

44 Air-Driven Micro-Ultracentrifugation Order Online Airfuge Air-Driven Micro-Ultracentrifuge Rotor Summary Rotor Part Maximum Maximum Number Tubes Rotor Approx. Rotor Biosafe Type Number Speed Force (g) Volume/Size Capacity Accel. Angle (rpm) at r max (ml)** Time* (min:sec) Fixed-Angle Rotors A , ,000 6 x 180 μl 1,080 μl 0:30-1:00 18 No Fast pelleting of very small samples. A-100/ , ,000 6 x 240 μl 1,440 μl 0:30-1:00 30 No Fast pelleting of very small samples. A-100/ , ,000 6 x 175 μl 1,050 μl 0:30-1:00 18 No Fast pelleting of very small samples. A , ,000 4 x 450 μl 1,800 μl 0:30-1:00 30 No Fast pelleting of very small samples. Chylomicron Rotor, Silver ACR , ,000 N/A 3.5 ml 0:30-1:00 No Rapid clarification of lipemic blood samples. Batch Rotor 3 Batch Rotor , ,000 N/A 7 ml 1:00 No Rapid processing of fruit paste. Electron Microscopy Particle-Counting Rotor EM , ,000 6 x 100 μl 600 μl 1:00 No Sedimenting particles onto filter membranes, glass cover slides and microscope grids. ** To maximum speed, rotor fully loaded. ** Unless otherwise noted. Beckman Coulter Benchtop and Microcentrifugation 3-3

45 A x 180 μl A-110 r min (9.5 mm) r av (12.1 mm) r max (14.7 mm) Fixed-Angle Rotor, Aluminum, Red For use in the Airfuge Air-Driven Micro-Ultracentrifuge. Major application: Fast pelleting of very small samples. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 110, , x 180 μl 1,080 μl 5 x 20 mm 18 No A-110 Rotor. Fixed-Angle Rotor with 18 angle, aluminum, anodized red. Includes 1 box of polyallomer tubes. Tubes and Bottles Tube Style/Material Usable Volume Part No. Quantity Size g-force k Factor Max. Speed (μl) (mm) Open-Top Tubes Polyallomer x , ,000 Polyethylene x , ,000 Ultra-Clear x , ,000 3 Rotor Supplies Rotor Caps, polyethylene, package of Tube Rack for 5-mm diameter Tubes Tube Cap Strip, 6-place, package of Rotor Base Bushing, white Rotor Stand/Vise A-100/30 6 x 240 μl A-100/30 r min (8.8 mm) r av (13.2 mm) r max (17.6 mm) Fixed-Angle Rotor, Aluminum, Blue For use in Airfuge Air-Driven Micro-Ultracentrifuge. Major application: Fast pelleting of very small samples. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 92, , x 240 μl 1,440 μl 5 x 20 mm 30 No A-100/30 Rotor. Fixed-Angle Rotor with 30 angle, anodized blue. Includes 1 box of Polyallomer Tubes. Tubes and Bottles Tube Style/Material Usable Volume Part No. Quantity Size g-force k Factor Max. Speed (μl) (mm) Open-Top Tubes Polyallomer x , ,000 Polyethylene x , ,000 Ultra-Clear x , ,000 Rotor Supplies Rotor Caps, polyethylene, package of Tube Rack for 5-mm diameter Tubes Tube Cap Strip, 6-place, package of Rotor Stand/Vise 3-4 Beckman Coulter Benchtop and Microcentrifugation

46 A-100/18 6 x 175 μl Order Online A-100/18 r min (9.5 mm) r av (12.1 mm) r max (14.7 mm) Fixed-Angle Rotor, Aluminum, Silver For use in the Airfuge Air-Driven Micro-Ultracentrifuge. Major application: Fast pelleting of very small samples. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 95, , x 175 μl 1,050 μl 5 x 20 mm 18 No A-100/18 Rotor. Fixed-Angle Rotor with 18 angle, aluminum, anodized silver. Includes 1 box of Polyallomer Tubes. Tubes and Bottles Tube Style/Material Usable Volume Part No. Quantity Size g-force k Factor Max. Speed (μl) (mm) Open-Top Tubes Polyallomer x , ,000 Polyethylene x , ,000 Ultra-Clear x , ,000 Rotor Supplies Rotor Caps, polyethylene, package of Tube Rack for 5-mm diameter tubes Tube Cap Strip, 6-place, package of Rotor Base Bushing, white Rotor Stand/Vise 3 A-95 4 x 450 μl A-95 r min (8.9 mm) r av (13.3 mm) r max (17.6 mm) Fixed-Angle Rotor, Aluminum, Black For use in Airfuge Air-Driven Micro-Ultracentrifuge. Major application: Fast pelleting of very small samples. Max. RPM Max. g k Factor Number of Tubes Rotor Capacity Volume/Size 30 95, , x 450 μl 1,800 μl 8 x 20 mm No A-95 Rotor. Fixed-Angle Rotor with 30 angle, aluminum, anodized black. Includes 1 box of Ultra-Clear Tubes. Tubes and Bottles Tube Style/Material Usable Volume Part No. Quantity Size g-force k Factor Max. Speed (μl) (mm) Open-Top Tubes Ultra-Clear x ,000 Rotor Supplies Rotor Caps, polyethylene, package of Tube Rack for 8-mm diameter Tubes Tube Cap Strip, 6-place, package of Rotor Base Bushing, white Rotor Stand/Vise Beckman Coulter Benchtop and Microcentrifugation 3-5

47 ACR ml/2.4 ml ACR-90 ACR-90 Chylomicron Rotor, Silver For use in the Airfuge Air-Driven Micro-Ultracentrifuge. Major application: Rapid clarification of lipemic blood samples. Max. RPM Max. g k Factor Rotor Capacity Yield Volume/Size (ml) 90, , ml , , ml 1.4 No ACR-90 Chylomicron Rotor, anodized aluminum. Consists of a Supporting Base, a Disposable Polyethylene Liner, and Stainless Steel Lid. Includes Rotor Stand/Vise, one package Liners, Adapter for Liner. Rotor Supplies Liner, polyethylene, 3.5 ml capacity, package of Liner, polyethylene, 2.4 ml capacity, package of 100 (Adapter required) Adapter for Liners, reusable Disposable Loading and Unloading Pipette, package of Rotor Bushing, white Rotor Stand/Vise Spinkote Lubricant Batch Rotor 7 ml Batch Rotor Batch Rotor, Aluminum, Silver For use in the Airfuge Air-Driven Micro-Ultracentrifuge. Major application: Rapid processing of fruit paste. Max. RPM Max. g Minimum Maximum k Factor Fill Volume Fill Volume 90, ,000 2 ml 7 ml 55 at 7 ml 8 at 2 ml No Batch Rotor, aluminum, for 2- to 7-mL volumes. Sample is placed in the rotor body itself or in one of two types of polyethylene liners. A plastic window disk is placed between the rotor and the lid to contain samples during centrifugation. Includes Rotor Stand/Vise and one each O-ring, Bushing. Rotor Supplies Shell Liner, polyethylene, large opening, 7-mL capacity, package of Dome Liner, polyethylene, small opening, 7-mL capacity, package of Rotor Window Disk Rotor O-ring Bushing, red 3-6 Beckman Coulter Benchtop and Microcentrifugation

48 EM-90 6 x 100 μl Order Online EM-90 EM-90 Particle Counting Rotor, Silver For use in the Airfuge Air-Driven Micro-Ultracentrifuge. Major applications: Sedimenting particles onto filter membranes, glass cover slides, and microscope grids. Max. rpm Max. g Maximum Rotor Capacity Volume 90, ,000 6 x 100 μl 600 μl No EM-90 Electron Microscopy Particle Counting Rotor. Includes 5-mm Core with 600 μl capacity (100 μl per cell); 3-mm Core with 360 μl capacity (60 μl per cell); Rotor Stand/Vise, Gaskets, Sector Core Removal Bolt, Template, one O-ring, one Bushing. Rotor Supplies Sector Core, 3 mm Sector Core, 5 mm Rotor Gaskets, package of Bushing, red Lid Core Cover 3 Flat Gasket Sector Core (3 mm or 5 mm) Rotor Base Stand Beckman Coulter Benchtop and Microcentrifugation 3-7

49 Tubes and Bottles Tubes and Bottles for Every Application No single tube design or material will meet all application requirements. A number of factors should be considered when a supply of tubes is ordered: the par ticu lar technique to be used, the nature of the sample and any solvent or gradient media, the desirability of reusing the tubes, and certain convenience factors. The properties listed below provide a guide for anyone involved in the tube se lection process. Strength and Flexibility, to resist permanent deformation even when run in fixed angle rotors without tube caps Chemical Resistance to a wide range of bases, acids, and solvents T ransparency, to permit a clear view of fractions and bands after centrifugation Thin enough to be sliced or punctured after centrifugation for fraction collection Impermeable to Water, to prevent aqueous solutions from permeating the tube wall and reaching the rotor cavity Surface Properties that prevent the adherence of nucleic acids and proteins T emperature Tolerance throughout a wide range of operating temperatures, without deforming at high temperatures or cracking when used close to 0 C Autoclavable, for convenient sterilization and reuse Contaminant-fr ee, to avoid leaching extraneous materials into the sample, especially materials visible in the sensitive nm range Odor -free, for pleasant handling 4 The full line of Beckman Coulter tubes includes a number of tube materials, each with its own distinct combination of prop erties to meet a variety of application requirements. Available are transparent, translucent, and opaque tubes; tubes that can be sliced or punctured; tubes that can be sterilized and reused; and tubes that are resistant to a variety of chemical com pounds. Beckman Coulter Benchtop and Microcentrifugation 4-1

50 Tubes and Bottles 4 Tube Selection Considerations Compatibility of Tube Material with Solvents and Sample The chemical compatibility of the tube materials with the gradient-forming medium or other solvent is a prime consideration. Neutral sucrose and salt solutions cause no problem. But alkaline solutions, such as those frequently used for the separation of single-stranded forms of DNA, cannot be used in Ultra-Clear tubes or polycarbonate tubes and bottles. Some times DMSO is used in preparation of sucrose gradients for sedimentation of denatured RNA. Poly carbonate and Ultra-Clear tubes are incompatible with DMSO, so polyallomer tubes should be used. The last column of the Quick Reference Chart to Tube Materials and Their Properties on page 3-4 gives some guidelines to the chemical resistances of the various tube materials. It must be em pha sized, however, that other conditions of centrifugation (g-force, duration of run, etc.) have considerable effect on how well a tube material will withstand a particular solvent. Beckman Coulter publication IN-175, Chemical Resistances for Beckman Coulter Centrifugation Products (found on the Beckman Coulter web site at labresources/centrifuges/chemres.asp) provides more detailed information about the chemical re sis tances of the various tube ma te rials. The wisest course is to test any ques tionable com bi nation under operating con ditions before making the actual run. The type of sample, in some cases, will affect selection of a specific tube material. DNA, in its denatured or singlestranded form, will adhere to the surface of some tube materials. Polyallomer would be the best choice. (Most of this work is done in highly alkaline media which are in com patible with polycarbonate.) Lipoprotein separations are most often done in Ultra-Clear tubes because they are clear and sliceable; these properties simplify fraction location and recovery by tube slicing. When small lipoprotein samples are to be recovered by a fractionating device and cl ear tubes are desirable, there are alternatives: cellulose propionate, polycarbonate, and Ultra- Clear tubes. Hazardous materials, either pathogenic or radioactive, should be centrifuged with extreme care. All possible precautions must be taken to avoid leakage of the sample into the rotor cavity during centrifugation. To determine the optimum tube material for your specific sample and gradient medium, refer to the quick reference chart on page 3-4. Gradient Formation and Fractionation When choosing a tube for a density gradient run, some thought should be given to gradient formation and fractionation. If the bands or zones formed during centrifugation are indistinct, they may not be visible through a translucent material such as polyallomer. If optimum band visualization is important, Ultra-Clear tubes or tubes of polycarbonate or cellulose propionate should be used. Whenever collection of bands or zones must be done by puncturing the tube or slicing, a thin, flexible tube wall is required. Ultra-Clear or polyallomer tubes should be used, depending on the need for transparency. As there are currently no wettable plastic centrifuge tubes available, gradients should be loaded into plastic tubes from the bottom up to avoid mixing. High Temperature Centrifugation Although modern centrifuges and rotors can operate at tempera tures as high as 45 C, one cannot assume that every tube can be safely run over 25 C. Stainless steel and glass are the only materials which will not experience some defor mation when subjected to high temperatures and long cen trifu gation times. Plastic tubes undergo some degree of softening at temperatures higher than 25 C. Whether or not this will cause permanent deformation is not a question of temperature alone. The centrifugal force field used, the duration of the cen trifu gation, the type of rotor, and even the tube angle all have an effect. It s obviously impossible to give exact temperature limits for plastic tubes when so many other variables are involved. The safest policy is to pretest the tubes under the actual experimental conditions, but with water, rather than a valuable sample. Tube Sizes Tube sizes as indicated in the following charts are nominal sizes, and may vary somewhat from actual filling capacities. If a thickwall tube is run uncapped, the maximum filling volume will depend on the tube angle of the rotor to be used. See appropriate rotor instruction manuals for maximum filling levels of tubes. Tube Cleaning, Sterilization, and Reuse 4-2 Beckman Coulter Benchtop and Microcentrifugation

51 Tubes and Bottles Order Online If tubes are to be reused, special care must be taken during cleaning and sterilization. All tubes can be washed by hand with a mild detergent such as Solution 555 diluted 5-to-1 or 10-to-1 with water. This is particularly important for poly car bonate tubes and bottles which should not be exposed to a detergent with a ph higher than 8. Tubes and bottles should not be washed in commercial dishwashers as the detergents and high tem pera tures are too harsh. Solvents such as alcohol or acetone react unfavorably with many tube materials. If an organic solvent must be used in the cleaning procedure, consult bulletin IN-175 for a table of tube material/solvent compatibilities (or review the same document on the Beckman Coulter web site at beckman.com/resourcecenter/labresources/centrifuges/ chemres.asp). The method chosen for sterilization has direct bearing on the number of reuses one can expect from a tube. Tubes and bottles of polyallomer, polyethylene, and glass can all be autoclaved, although in general, cold sterilization methods are not as harsh as autoclaving. Cold sterilization is recommended for both polycarbonate and Ultra-Clear. If maximum reuse is a major consideration, either polyallomer (preferably thickwall) or polycarbonate tubes and bottles should be selected, and cold sterilization methods used. If these tubes are run completely filled in swinging bucket rotors, most of them can be reused a number of times. Chances of permanent de for mation will be greater whenever the tubes are run in fixed angle rotors, without caps, and/or partially filled. All of these con ditions tend to stress the centripetal edge of the tube unduly. All tubes that have been used or autoclaved previously must be individually examined for signs of deformation or cracking before using them again. Tube Closures When other considerations have been resolved, convenience may be a deciding factor. Without a doubt, the most convenient tube closure is none at all; none are required for tubes run in swinging bucket rotors. For tubes run in fixed angle rotors, alternatives to the standard tube cap assemblies are available. Bottles have three-piece cap assemblies which are easier to use than the more complex tube cap assemblies. Polycarbonate bottles are available for general-purpose fixed angle rotors, and are used frequently for differential cen trifugation where band re cov ery is not a problem. Thickwall tubes can be run in all fixed angle rotors without caps, provided they are partially filled. (Refer to rotor manuals for more information on fill volumes.) When closed tubes are required, Beckman Coulter offers some innovative and con ve nient options. 4 Beckman Coulter Benchtop and Microcentrifugation 4-3

52 Tubes and Bottles A Quick-Reference Chart to Tube Materials and Their Properties 4 Property Thinwall Thickwall Ultra-Clear Polycarbonate Polypropylene Polyethylene Cellulose Polyallomer Polyallomer Propionate Optical transparent translucent transparent transparent translucent transparent/translucent transparent Autoclaveable yes yes no no yes no no Puncturable yes no yes no no yes no Sliceable yes no* yes no* no no no* Reusable no yes no yes yes yes no Acids (dilute S S S S S S S or weak) Acids (strong) U S U U S S U Alcohols (aliphatic) U S U U S S U Aldehydes M M S M M S U Bases S S U U S S U Esters U M U U M S M Hydrocarbons U M U U S U S (aliphatic) Hydrocarbons U U U U M M S (aromatic and halogenated) Ketones U M U U M M U Oxidizing U U U M M M M Agents (strong) Salts S S M M S S S S = satisfactory resistance M = marginal resistance U = unsatisfactory resistance ** Polyallomer, polycarbonate, and cellulose propionate tubes with diameters of 5 to 13 mm may be sliced using the CentriTube Slicer (part number ) and appropriate adapter plate. Note: This information has been consolidated from a number of sources and is provided only as a guide to the selection of tube materials. Soak tests at 1 g (at 20 C) established the data for most of the materials; reactions may vary under the stress of centrifugation, or with extended contact or temperature variations. To prevent failure and loss of valuable sample, ALWAYS TEST SOLUTIONS UNDER OPERATING CONDITIONS BEFORE USE. Warning: Do not use flammable substances in or near an operating centrifuge. 4-4 Beckman Coulter Benchtop and Microcentrifugation

53 Tubes and Bottles Order Online General Filling and Sealing Requirements for Tubes and Bottles Tube or Bottle Swinging-Bucket Rotors Fixed-Angle Rotors Polyallomer Thinwall tubes Within 2 to 3 mm of top Full with cap Thickwall tubes At least 1/2 full 1/2 full to max. capless level or full with cap Quick-Seal tubes Full and heat-sealed Full and heat-sealed Bottles Min. to max. (see rotor manual) 1/2 full to max. (see rotor manual) with screw-on cap with screw-on cap or cap assembly or cap assembly Ultra-Clear Open-top tubes Within 2 to 3 mm of top Full with cap Quick-Seal tubes Not used Full and heat-sealed Polycarbonate Thickwall tubes At least 1/2 full 1/2 full capless level or full with cap or cap assembly Bottles At least 1/2 full Min. to max. (see rotor manual) with screw-on cap or cap assembly Stainless Steel Tubes Any level Any level with cap or cap assembly Polypropylene Tubes and bottles At least 1/2 full 1/2 to max. capless level or full with cap or cap assembly Polyethylene Tubes At least 1/2 full 1/2 to max. capless level or full with cap or cap assembly Teflon Tubes and bottles At least 1/2 full 1/2 full to max. capless level or full with cap 4 Beckman Coulter Benchtop and Microcentrifugation 4-5

54 Tubes and Bottles Bottles Used in Benchtop Centrifuges 4 Nominal Size Material Bottle & Bottle with Bottle Insert O-ring Screw Cap Capacity mm Cap Assy Screw Cap Only Only Only 10 ml 16 x 80 PC N.A N.A. N.A. N.A. N.A. 10 ml 16.1 x 81.1 PA N.A N.A. N.A. N.A. N.A. 10 ml 16.1 x 81.1 T N.A N.A. N.A. N.A. N.A ml 25 x 89 PC N.A ml 25.3 x 92 PA N.A. N.A. N.A. N.A. N.A. 30 ml 25.3 x 92 PC N.A N.A. N.A. N.A. N.A. 40 ml 29 x 104 PC N.A N.A. N.A. N.A. N.A. 50 ml 28.5 x 107 T* N.A N.A. N.A. N.A. N.A. 50 ml 29 x 104 PA N.A ml 29 x 104 PC N.A N.A ml 38 x 102 PC N.A ml 38 x 104 PC N.A. N.A. N.A. N.A. 85 ml 38 x 104 PP N.A N.A. N.A. N.A. N.A N.A. N.A. N.A. N.A. 100 ml 38 x 102 PP N.A N.A ml 62 x 120 W PC N.A N.A ml 62 x 122 W PP N.A N.A ml round-bottom 62 x 136 PC N.A N.A. N.A. N.A ml 69 x 160 PC N.A N.A. N.A. N.A. 500 ml 69 x 160 PP N.A ml 69 x 160 W PP N.A N.A. N.A. N.A. N.A. 500 ml 85 x 135 PC N.A N.A. N.A. N.A. N.A. 500 ml 85 x 135 PP N.A N.A. N.A. N.A. N.A. 750 ml 96 x 130 PC N.A N.A. N.A ml 96 x 130 PP N.A N.A. N.A PA = Polyallomer PC = Polycarbonate PE = Polyethylene PP = Polypropylene T = Teflon W = Wide-Mouth N.A. = Not Available ** With high-speed screw cap. 4-6 Beckman Coulter Benchtop and Microcentrifugation

55 Tubes and Bottles Order Online Adapters for Non-Beckman Coulter Tubes/Bottles* Rotor Tube Vol. Size (mm) # of Places Part Number SX ml 7 x 19 mm ml 12 x 75 mm ml Hemolyse/RIA ml 13 x 75 mm ml 12 x 100 mm ml 16 x 100 mm ml 17 mm ml 16.1 x 81.1 mm /15 ml 17 x 110 mm ml 16 x 125 mm ml 17 x 120 mm ml 24 x 100 mm ml 28.5 x 100 mm ml 28.5 x 107 mm ml 28.5 x 100 mm ml 34 x 100 mm /94 ml 38 x 112 mm /100 ml 44 x 100 mm ml 50.5 x 99 mm ml 56.5 x 99 mm ml 61.8 x 125 mm FX6100 Conical 15 ml 17 x 100 mm ml ml Conical 50 ml 28.5 x 120 mm FX μl H μl SX μl S μl F μl Adapters for Glass Tubes in Beckman Coulter Rotors* Rotor Tube Volume Tube Material Adapter Part Number SX /100 ml Glass ml Glass ml Glass with Open-top or Screw-on Cap ml Glass ** Check with tube manufacturer for maximum allowable g-force. Beckman Coulter Benchtop and Microcentrifugation 4-7

56 Tubes and Bottles Tubes and Bottles Used in Benchtop Centrifuges and Microcentrifuges 4 Nominal Nominal Inches Part Rotors Filling Size No. Capacity (ml) (mm) Open-Top Tubes, Polyallomer, Thickwall* x x F x x F1010, SX4750, SX4750A, TS , FX x x F x x F0485, F0685, FX6100 Open-Top Tubes, Polycarbonate, Thickwall* x x F1010, TS x x F1010, SX4750, SX4750A, TS , FX x x SX4750, SX4750A, FX x x F0485, F0650, F0850, F0685, SX4750, SX4750A, TA-14-50, TA , FX x x (conical, grad.) F0650, F0850, SX4750, SX4750A, FX6100, TA x x F0485, F0685, FX6100 Open-Top Tubes, Polyallomer x x F x x F0485, F0685 Open-Top Tubes, Polyethylene, Plain x x F1202, F2402H, F241.5, F241.5P, F301.5, F3602, F40.25, FX301.5, H6002, S241.5, SX241.5, TA x x F1202, F2402H, F241.5, F241.5P, F301.5, F3602, F40.25, FX301.5, H6002, S241.5, SX241.5, TA x x SX4750, SX4750A, FX6100 Open-Top Tubes, Polyethylene, Heparin/Lithium Coated x x F1202, F2402H, F241.5, F241.5P, F301.5, F3602, F40.25, FX301.5, H6002, S241.5, SX241.5, TA x x F1202, F2402H, F241.5, F241.5P, F301.5, F3602, F40.25, FX301.5, H6002, S241.5, SX241.5, TA Open-Top Tubes, Polyethylene, Heparin/Lithium/Fluoride Coated x x F1202, F2402H, F241.5, F241.5P, F301.5, F3602, F40.25, FX301.5, H6002, S241.5, SX241.5, TA x x F1202, F2402H, F241.5, F241.5P, F301.5, F3602, F40.25, FX301.5, H6002, S241.5, SX241.5, TA Open-Top Tubes, Polypropylene x x SX4750, SX4750A, FX x x F0485, F0650, F0850, F0685, SX4750, SX4750A, TA-14-50, TA , FX6100 Open Top Tubes, UltraClear x x F x x F0630 Tubes with Snap-On Attached Caps, Polyallomer x x Natural F1202, F2402H, F241.5, F241.5P, F301.5, F3602, FX301.5, SX4750, H6002, S241.5, SX241.5, TA , TS , FX6100 ** Nominal fi ll volume for these tubes can vary from 6.0 to 10.0 ml, depending on the rotor in which it is used due to different tube angles. 4-8 Beckman Coulter Benchtop and Microcentrifugation

57 Tubes and Bottles Order Online Nominal Nominal Inches Part Rotors Filling Size No. Capacity (ml) (mm) Tubes with Snap-On Caps, Polycarbonate* x x F0850, SX4750, SX4750A, TA-14-50, TA Tubes with Snap-On Caps, Polyethylene x x Natural F1202, F2402H, F241.5, F241.5P, F3602, SX4750, SX4750A, H6002, S241.5, SX241.5, TA , FX6100 Tubes with Snap-On Caps, Polypropylene x x Natural SX4750, SX4750A, S x x Blue SX4750, SX4750A, S x x Green SX4750, SX4750A, S x x Yellow SX4750, SX4750A, S x x Orange SX4750, SX4750A, S4180 Tubes with Snap-On Caps, Polypropylene x x F1202, F2402H, F241.5, F241.5P, F3602, H x x F1202, F2402H, H x x Natural F1202, F2402H, F241.5, F241.5P, F301.5, F3602, FX301.5, SX4750, SX4750A, H6002, S241.5, SX241.5, S4180, TA , FX x x F0850, SX4750, SX4750A, TA , TA Bottles with Cap Assemblies, Polycarbonate x x (polycarbonate, F0650, F0850, SX4750, SX4750A, FX6100, TA graduated) Bottles with Screw-on Caps, Polycarbonate x x (polycarbonate SX4750, SX4750A, TA bottle with screw cap) Bottles with Screw-on Caps, Polypropylene x x (polypropylene SX4750, SX4750A, TA bottle with screw cap) Bio-Vial Tubes 4 14 x x Polypropylene SX4750, SX4750A Bottles with Cap Assemblies, Polyallomer x x F0650, F0850, SX4750A, TA , TA-14-50, SX x x F0650, F0850, SX4750A, TA , TA-14-50, SX ** Nominal fi ll volume for these tubes can vary from 6.0 to 10.0 ml, depending on the rotor in which it is used due to different tube angles. Beckman Coulter Benchtop and Microcentrifugation 4-9

58 Tubes and Bottles 4 Nominal Nominal Inches Part Rotors Filling Size No. Capacity (ml) (mm) Bottles with Cap Assemblies, Polycarbonate x x F x x SX4750, SX4750A x x F0650, F0850, SX4750A, TA , TA x x F0485, F0685, FX x x (high-speed cap) F0485, F0685, S4180, FX x x (wide mouth) SX4750 Bottles with Cap Assemblies, Polypropylene x x (wide mouth) SX x x (wide mouth) SX4750, TA , TS x x SX4750 Bottles with Screw-on Caps, Polyallomer x x F1010, S4180, TS x x F x x F0485, F0650, F0685, F0850, SX4750, SX4750A, TA , TA-14-50, FX6100 Bottles with Screw-on Caps, Polycarbonate x x F1010, S4180, TS x x F x x F0485, F0650, F0685, F0850, SX4750, SX4750A, TA , TA-14-50, FX x x F0485, S x x (conical) SX4750, SX4750A, TA x x (wide mouth) SX4750, SX4750A, TA , TS x x SX x x TS x x SX4750 Bottles with Screw-on Caps, Polypropylene x x F0685, FX x x F0485, S x x F0485, F0685, FX x x S x x (conical) SX4750, SX4750A, TA x x SX x x TS x x SX4750 Bottles with Screw-on Caps, Teflon x x F x x F0650, F0850, SX4750, SX4750A 4-10 Beckman Coulter Benchtop and Microcentrifugation

59 Tools and Supplies 5 Beckman Coulter Benchtop and Microcentrifugation 5-1

60 Tools and Supplies Rotor Cleaning Kit Rotor Cleaning Kit. Contains two 946-mL bottles of Solution 555 Rotor Cleaning Concentrate, Rotor Cleaning Brush, and Rotor Cleaning Brush Replacement Parts/Supplies Solution 555 Rotor Cleaning Concentrate (min. order two Bottles) Rotor Cleaning Brush, 5 8-in. (16 mm) and 1-in. (25.4 mm), for Rotor Cavity diameters from 7 16-in. (11 mm) to 1-in. (25.4 mm) (min. order three Brushes) Rotor Cleaning Brush, in. (32 mm) and in. (38 mm), for Rotor Cavity diameters from 1-in. (25.4 mm) to in. (38 mm) (min. order three Brushes) Beckman Coulter Benchtop and Microcentrifugation