Leica M205 A, M205 C, M165 C, and M125. FusionOptics : High Resolution and High Depth of Field Simultaneously

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1 Leica M205 A, M205 C, M165 C, and M125 FusionOptics : High Resolution and High Depth of Field Simultaneously

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3 Redefining High Performance Defying the inherent limitations of the stereomicroscope Ever since their invention, stereomicroscopes have operated according to the principles discovered by Ernst Abbe* and Horatio S. Greenough**. For over a century, optics designers and engineers have pushed magnification, resolution, and image fidelity to the limits of modern optical principles. Stereomicroscope optical technology advancement, over the years, has always been constrained by interrelated factors: the higher a microscope s resolution, the lower the available working distance. If one increases working distance and resolution using higher magnification objectives and lenses, the three-dimensional image seen through the eyepieces becomes distorted a sphere becomes an ellipse and a flat surface curves toward the observer. Breaking the limits of optical resolution Leica FusionOptics Leica Microsystems introduces the groundbreaking Leica M205A, M205 C, M165 C and M125 stereomicroscopes. The Leica M205 A and M205 C are the world s first stereomicroscopes with a magnification zoom range of 20.5x. This, however, is not the only technological advancement. With Leica Microsystem s new FusionOptics, the Leica M205 A and M205 C overcomes the previous limits of optical technology. In addition to increased magnification, the resolution has increased to 1050 lp/mm, which corresponds to a structure size of 476 nm without sacrificing depth of field. This new level of high performance benefits everyday work at the stereomicroscope. View samples with a new freedom of movement and discover detail that has never been seen before with a stereomicroscope. *Ernst Abbe was a German mathematician and physicist who made several of the most important contributions to the design of lenses for optical microscopy during the late 1800 s. **Horatio S. Greenough, an American instrument designer, introduced a novel design in the early 1890 s, and became the forefather of modern stereomicroscopes.

4 The Best Information from Every Image Fusion of high resolution and high depth of field The design principle behind the new Leica M205 A and M205 C is FusionOptics : The microscope assigns a different task to each of the microscope s two beam paths. The right channel contains an image with very high resolution, while the left channel provides very high depth of field. The best information from both sources composes one image with rich detail. High magnification with great ergonomic benefits Conventional stereomicroscopy gives users a choice: either high resolution and richness of detail, or a larger working distance for easy sample manipulation. Traditionally, the higher a microscope s resolution, the less working space there is between a sample and the microscope s objective. With a 1 objective, the Leica M205 A and M205 C reaches magnification ranges that previously required high-magnification objectives. At the same magnification, the Leica M205 A and M205 C resolve structures of less than one micrometer in size, offers a depth of field of 2.68 mm and at the same time provides 61.5 mm of free working distance for sample manipulation. Sorting and processing even the smallest details are performed easily without changing objectives. APO-corrected accessories for enhanced performance To take full advantage of the performance capability of the new Leica M205 A, M205 C, M165 C, and M125 stereomicroscopes, all new Leica M-series objectives, ergotubes, and accessories are apochromatically corrected. Unwanted color seams and curvature of field are things of the past.

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6 Ready for Your Tasks Put Leica Microsystems to the Test! Whenever human lives may be affected, technology must be 100% reliable. For example, airplane autopilots and airbag mechanisms are subject to stringent materials testing. A wide variety of methods are used to measure the behavior and characteristics of standard material samples or finished parts (component testing) under thermal, mechanical, and chemical stress. Materials are checked for purity, faults, and load capacity. The new Leica M205 A and M205 C allows experts in the materials testing lab to see smaller structures than ever before. Leica s one-of-a-kind FusionOptics is the first stereomicroscope technology to enable both higher depth of field and resolution, simultaneously. The electronically encoded zoom allows a tester to eliminate sources of error in measurement applications and to reproduce test settings at any time. Using the Leica Application Suite (LAS) software, the tester can access all relevant data, store images in an image database, and measure and analyze the data using a computer. Leica Microsystems two new illumination sources, the Leica LED5000 MCI (Multi Contrast Illumination) and LED5000 RL (Ring Light), provide a wide range of adjustment and automation options. The Leica M205 A, M205 C, M165 C, and M125 illumination source, and automated mechanical stage form a complete system. The user can program various test cycles to run in a fully automated process. Time-savings, in addition to error prevention, are important advantages of the new Leica M205 A, M205 C, M165 C, and M125 stereomicroscopes. 6

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8 Leica Application Suite: User friendly Imaging Solution Integrated, complete solution Leica Application Suite (LAS) software integrates an automated stereomicroscope, the complete line of Leica DFC digital cameras, and Leica Microsystems software into a single workstation to provide a user-friendly imaging solution with unsurpassed, consistent performance. LAS is ideal for a wide variety of tasks and features a comprehensive range of image processing functions to make it faster and easier to visualize, process, measure, and document digital images. By providing all the tools needed for the installed applications to communicate with each other and with peripheral devices connected to the computer, LAS is an intuitive solution that makes production QC, research, and industrial analysis easier. Features at a glance Increases productivity by integrating stereomicroscopes, digital cameras, and application software and coordinates imaging tasks via an intelligent control system. Automates digital photomicrography when combined with the computer-aided functions of Leica Microsystems new stereomicroscopes. One software application is all that is needed for viewing, capturing, storing, and annotating high-quality images in a thumbnail gallery. Highly modular can be configured for routine QC applications as well as for new research methods.

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10 Technical Highlights Leica M205 A, M205 C, M165 C & M125 The digital display of the Leica M205 A shows all essential settings at a glance The design of the new focusing column provides extreme stablility and takes the high magnification into account. Leica M205 A and M205 C stereomicroscope with the highest zoom ratio 20.5 : 1 zoom allows overview and detail observation using one instrument. Numerical aperture of 0.35 ( with 2 planapochromatic objective ) One-of-a-kind resolution of 1050 lp / mm enables resolution of structures down to 476 nm. Rigid, sturdy mechanical structure Stable mechanical structure supports high optical performance. Integrated cable duct and completely integrated electronics keep the workspace neat and clean. Encoding and Motorization M165 C / M205 C: Continuous electronic readout of the magnification and iris diaphragm M205 A: Motorization of magnification and iris diaphragm Internal instrument encoding provides continuous electronic readout of the magnification for fast calibration. The objective nosepiece meets even the most stringent requirements for the magnification range without laborious refocusing. Modularity The new Leica M205 C, M165 C and M125 stereomicroscopes can combine with many existing Leica Microsystems components and accessories. Choose from a wide selection of objectives, stands, cameras, illuminators, and other accessories. A solution is available for virtually every application, from routine QC to research. Completely integrated illuminator New illumination components seamlessly integrate with the complete system. Complete control and reproducibility of settings. All settings are easy to use. Leica LED5000 RL: fully integrated ring light, completely controlled using Leica Application Suite (LAS).

11 Relaxed work Trinocular tube with 30 viewing angle. Modular components provide maximum viewing comfort for different microscope users. Leica Microsystems revolutionary FusionOptics (M205 A and M205 C only) Right channel with high resolution. Left channel with large depth of field. The information from both channels creates previously unattainable brilliance and depth of field. Diopter features notched increments Prevents accidental readjustment of dioptric correction. Replaceable eyecups for the highest standards of hygiene. Apochromatically corrected optics Optics are corrected for chromatic aberrations and flatness. No unwanted color seams or distortion during observation, image capturing or image evaluation. Convenient operation under the microscope Allows greater working distance for new and existing Leica Microsystems optics. Provides even more room for working with samples. Field number 23 enables a large-surface overview of the sample. Leica FusionOptics at work: Left and right channels are separately responsible for depth of field and resolution. A 30 viewing angle ensures a relaxed head posture. The 20 notched increments of the eyepieces prevent accidental readjustment of the dioptric correction. Leica Microsystem s new, apochromatic objectives prevent color seams, and field number 23 enables a large overview of the sample mm working distance gives plenty of working space under the objective. Leica Design by Christophe Apothéloz

12 With the user, for the user Leica Microsystems Leica Microsystems operates internationally in four divisions, where we rank with the market leaders. Life Science Division The Leica Microsystems Life Science Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems customers at the leading edge of science. Industry Division The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the microstructures in routine and research industrial applications, materials science, quality control, forensic science investigation, and educational applications. Biosystems Division The Leica Microsystems Biosystems Division brings histopathology labs and researchers the highest-quality, most comprehensive product range. From patient to pathologist, the range includes the ideal product for each histology step and high-productivity workflow solutions for the entire lab. With complete histology systems featuring innovative automation and Novocastra reagents, Leica Microsystems creates better patient care through rapid turnaround, diagnostic confidence, and close customer collaboration. Surgical Division The Leica Microsystems Surgical Division s focus is to partner with and support surgeons and their care of patients with the highest-quality, most innovative surgi cal microscope technology today and into the future. The statement by Ernst Leitz in 1907, with the user, for the user, describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life. Active worldwide Australia: North Ryde Tel Fax Austria: Vienna Tel Fax Belgium: Groot Bijgaarden Tel Fax Canada: Richmond Hill/Ontario Tel Fax Denmark: Herlev Tel Fax France: Rueil-Malmaison Tel Fax Germany: Wetzlar Tel Fax Italy: Milan Tel Fax Japan: Tokyo Tel Fax Korea: Seoul Tel Fax Netherlands: Rijswijk Tel Fax People s Rep. of China: Hong Kong Tel Fax Portugal: Lisbon Tel Fax Singapore Tel Fax Spain: Barcelona Tel Fax Sweden: Kista Tel Fax Switzerland: Heerbrugg Tel Fax United Kingdom: Milton Keynes Tel Fax USA: Bannockburn/lllinois Tel Fax and representatives in more than 100 countries Illustrations, descriptions and technical data are not binding and may be changed without notice. Printed on chlorine-free paper with a high content of recycled fiber. 10IDM10010US Leica Microsystems (Schweiz) AG CH-9435 Heerbrugg, 2008 Printed in Switzerland XI.2008 RDV

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