N.1 ION CHROMATOGRAPHY/HPLC MODULAR SYSTEM FOR ANIONS, CATIONS, TRANSITION METALS, PESTICIDES, PAH
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1 N.1 ION CHROMATOGRAPHY/HPLC MODULAR SYSTEM FOR ANIONS, CATIONS, TRANSITION METALS, PESTICIDES, PAH TECHNICAL DESCRIPTION INTEGRATED TECHNICAL SPECIFICATIONS Sulmona, 20/01/2009
2 1. Description of the Purposed Instrument Contents of this document 1.1. Hplc/Ic S Description of the Chromatograph S Content of the Supply 1.2. Assembly Scheme 2. Technical Characteristics Solvent delivery System Portlab SERIE II Conductivity Detector Portlab CD Ultrafluor 1200 Fluorescence detector UV-104M Dual Wavelenght Spectrophotometric Detector Rheodyne Injection Module Software Chrom&Spech per Windows Control and Data Acquisition ACS Suppressor PCI driver Board 8 channels PC Integrated ted
3 1. Description of Purposed Instrument 1.1. HPLC/IC S003.2 Portlab is the only Italian Company capable to manufacture and Purpose Ion Chromatograph and HPLC Systems available for every kind of application. All IC and HPLC manufactured by Portlab are produced in Modular Technology. It offers to all our customer the great advantage to own a completely expandible device. Such advantage is usually reserved only to advanced users of high cost devices. Portlab extends the modularity to all segments of products, including the economic segment! The lucky owner of Portlab IC and HPLC systems will have the possibility to add, step by step with his own budget plan, all components he desires to expand his device (such as extra pump, extra detector, column thermostate, electrodialysis suppressor, online degasser etc ) ADDITIONAL FEATURES: High Level Device without limitations Software Full version for 16 asinchronous independent channels Possibility to implement second and third independent hydraulic line The device is Customizable 100% Modular device, expandible till double quaternary gradient and unlimited number of installed detectors. Unlimited integrable accessories (degasser, column oven, automatic SPE block, autosampler etc ) Works in suppressed and non-suppressed mode Possibility to interface any other kind of detector from other producers (via ADC) Fully programmable. All peripherals are controlled via software Italian Production Free software upgrade for 10 years No compressed Gases needed
4 Description of the Chromatograph S003.2 The Chromatograph purposed for this tender is modular and vertically mounted. The solvent delivery module is composed by a free metal pumping system binary gradient, with 2 independent mechanisms pumping solvents inside a Dynamic Mixing Chamber in High Pressure. This configuration enable the possibility to use the solvent delivery system for binary gradients operations or for derivatization protocols, using one of the two mechanism as derivatizator pump. The mechanism is an High Performance HPLC unit and it is much more performant then low pressure gradient systems. The 4 check valves ensures fluidic stability and the back flush head dramatically extends the piston seals life. The injection system is 6 ways/ 2 positions Rheodyne type in peek synchronized for the start of the chromatogram. This module can be expanded in future adding the special AUTOBLOCK, completely software controlled, is capable to execute the most difficult operations. AUTOBLOCK is a Portlab exclusive, and enable possibility of including conditioning programs, washing and column/eluent change directly inside the queue list. It means that customer needs just to load the vials inside the sample rack and execute the list. The instrument automatically will: Clean the needle using water Choose the right columns and eluents and start conditioning Load the sample inside the sample loop and inject it once the system is stable Execute all sample is simultaneous, sequential or single mode Calibrate from standards Integrate peeks according to preloaded criterias and receiving results Printout reports Autoswith of the low pressure valve to the water for cleaning of the hydraulic lines prior to switch off Autoswitch off The CD-510 Microprocessor s Conductivity Detectors, shows the most advanced performaces in the world, with unique dinamic range ( us/cm). The thermostated flow cell, the fully programmable microprocessors function (gain, polarity, programs) makes the CD-510 the most sensitive, versatile and powerfull CD Detector ever created. Thanks to its fantastic performances, Portlab Devices works for Cation analysis in non suppressed mode. For anions, Portlab uses ACS chemical suppressor membrane technology, which ensures the best performances against and solid phase suppressor. The Fluorescence Detector, the Ultrafluor 1200, manufacturer for us by Agilent Technologies, is one of the best unit in the world. This unit includes internal driver protocol for driving without software. The UV-VIS VIS detector ector, 104M, is a detector that can show a fantastic stability, a very low noise level and a verry quick stabilization time. The software, Chrom&Spech per Windows, Allows the full control of the device, the data acquisition, the storage of the datas and the manipulations, the creation of personalized reports and many other things the software is installed on a Latest Generation workstation, inclusive of monitor and laserprinter. Al the instrument is stabilized through a UPS unit by APC. All purposed columns are suitable for the required applications. All Instrument s fluid path is manufactured in PEEK, ph compatible PORTLAB devices ensures analytical results according to the most important world analytical parameters (EPA, FDA, CNR)
5 This image does not correspond to the purposed unit
6 Content of the Supply 1 Pumping System composed by 2 mechanics SERIE II, + 1 MS16 Dinamic mixing Chamber till quaternary gradient 1 CD510 Microprocessor s Conductivity Detector, complete with Thermostated Cell, RS232c, Analog output, Syncronization and accessories. Includes ACS suppression Unit 1 Ultrafluor 1200 Fluorescence detector, complete with cell and pulsed xenon lamp 1 UV-VIS dual lamp detector, multiwavelenght, nm, with analytical cell 1 Column Kit Complete for PAH, Pesticides, Metals, Anions and Cation 1 Kit Standard complete for all applications 1 Injection rheodyne valve in peek with mounting brackets 1 Kit including 5 plastic bottles, tubes, cables, capillarars, sample loops (8 1x25 ul, 1x 10 ul, 1x 20 ul, 1x 50 ul, 1x 100 ul, 1x 250 ul, 1x 300 ul, 1x 400 ul), 1 Column Oven TS110 with holder barrels 1 PC. Monitor, Laserprinter, PCI driver board for control the instrument and APC UPS power surge for the whole instrument 1 Software Chrom&Spech per Windows with USB key for unlock,capable of simultaneous operations. Version FULL. 1 Spare parts kit for 3 years including all columns and standard purposed for the start up, a piston seal exchange parts, check valve exchange parts, 1 ACS suppressor complete, 1 deuterium and 1 tungsten lamp for replace inside the UV-VIS detector and a kit of capillars, ferulas, nuts and tools for the easy maintainance of the unit Assembly Scheme
7 SOFTWARE DATA PROCESSING INJECTION VALVE UV-VIS DETECTOR CD DETECTOR FLUORESCENCE DETECTOR DINAMIC MIXER PUMP 1 PUMP 2
8 2. Technical Characteristics Solvent delivery system PortLab Serie II Portlab Serie III Modular Analytical Pumps The size of the analytical HPLC pump is mere 30 cm wide. Two or more pumps and other components can be stacked to comprise a gradient HPLC system requiring minimal bench space in the crowded modern laboratory. Excellent pump performance The pumping method integrated with membrane pulse dumper ensures the most reliable, accurate and stable solvent delivery possible. A guaranteed flow rate precision within 0.1% assures good reproducibility of retention time. Pulse-free flow assures maximum detector sensitivity, thanks to high technology pistons and the membrane pulse Dumper integrated. Moreover, the high performance pump mechanic guarantee very high working pressures (around 450 bars for SS316 pump heads!) Extreme durability The duel check Valve system and the back flush pump head assures a long maintanance free pump life. The shappire piston is protected against salt precipitations by extra low pressure pump head. Front Panel and Extra features Front Panel allows users to set all needed informations, such as flow rate, type of pressure unit (bar or PSI), Low and High pressure limits, Pressure Autozero. Integrated leds and acoustic signals warning user in case of fault, leakage or other inconveniences. Special and unique feature allows user to set up Intelligent High pressure limit, so that one s it has been reached by pump it will stop, but then restart itself when the pressure comes down to 75% of the maximum limit. The pump can be controlled via Software using RS-232 protocol and can be paired for gradient operations. Special button disconnects pressure transducer and allows user to measure pressure only inside the mixing chamber (when gradient is performed). FEATURES: Digital HPLC pumps compatible with gradient operation Pulse free flow is generated using a low dead Volume pulse damper «900ul) with dual serial piston (50 pl per stroke).this is especially important in operation with flow sensitive RI and EC detectors. It is suitable far both analytical and preparative scale applications using interchangeable heads with flow from 0.01 to 10,00 ml/min or 0.04 to 40 ml/min respectively. Interchangeable pump heads with 316 SS or biocompatible PEEK liquid contact surfaces are available. Compatible with most commercial HPLC systems Superior support and warranty programs. Dual Self Flushing Pump Heads for Reduced Pulsation Real-time pressure monitoring with upper/lower pressure limits.
9 Remote 0 10 volt & remote 0 10 khz Flow Rate Control Input Simple Front Panel Key Pad Controls with LED Display Autoprime one button toggles flowrate to maximum for rapid solvent change Motor Stall Detector Dual ball and seat Check Valves for Consistent Flow Performance Prime Purge Valve RS-232 Serial Com Port for Complete Control & Status Monitoring Stainless Steel or PEEK Fluid Path PUMPING SYSTEM SERIES II Structure Material Pulsations Flow Precision TECHNICAL SPECIFICATIONS Rapid Refill Piston with Integrate Membrane Pulse Dumper 2 units Peek ± 1mL/min and 1,000 psi 0.2% RDS Flow Accuracy ± 1% Flow Gradient Accuracy Maximum Pressure Pressure Accuracy Pressure Settable Limits Integrated Fluid Cleaning Purge Valve Outlet Filter Data Transfer Protocol Weight Dimensions (LxHxD): DINAMIC MIXING CHAMBER MS-16 Structure Material ml/min.(every mechanism) between 1% 1 ml/min. and 1000 psi psi 1% over all range 2 (low and high) Yes (Piston Self Flush) Yes, Integratet on each mechanism 0,2 mm RS232 Cell Volume 1,2 µl Inlets 4 Integrated Pressure Trasducer Pressure Accuracy approx 15 Kg 320x165x260 mm Magnetic Rotative Sinamic Mixing Chamber 1 unit SS316 coated PTFE Yes, Linear till 350 bars 1% over all range Purge Valve Data Transfer Protocol Weight: Dimensions (LxHxD): Yes RS232 approx 10 Kg 320x165x260 mm
10 Pump Mechanism Serie II Conductivity Detector Portlab CD-510 Portlab CD-510 Features - The model 510 is a microprocessor based Conductivity Detector for routine or sophisticated trace-level applications. - It can be used with almost any HPLC system to give you the capability to analize anions, cations, transition metals and organic acids. - The built-in Autozero function permits you to work with high conductivity mobile phases. - It is possible to use the 510 simply by pressing a key. It can be used in both suppressed and non-suppressed modes of ion chromatography.
11 - The conductivity cell is located in a temperature controlled housing that provides enhanced baseline stability. - Some Additional features of the 510 CD are: - Gain, polarity, time constant, cell s temperature, event marker and Autozero are controlled from the front panel - Waterpfoof cell housing is separated from the main block of the detector. It provides easy access to the celll and protection of electronic elements from leakage of the mobile phase - Microprocessor-based electric ciruitry provides high sensitivity - Light and sound alarm for signal overload and temperature instability - Optional anion capillary suppressor - Active heat exchanger provides high temperature stability of eluent in the cell, which results in enhanced baseline stability. - Enables possibility to work using suppressed and non suppressed mode in a single run
12 TECHNICAL SPECIFICATIONS CONDUCTIVITY DETECTOR PORTLAB CD510 Detection Alternated Current, Frequency 10 KHz Range µs/cm in 999 steps Cell Material PEEK, operates ph Noise µs/cm over all range Drift Better then 0,05 us/hour Resolution 0,005 ns/cm Linearity 0,5 % over all range Electronic Noise and Baseline Noise < 0,1 ns/cm (without column) and 0,15 ns (with column) Display LCD 2 x 16 characters Time Costants 1,2, 3 & 4 Cell Thermostate C Temperature Increments ± 0,1 C Temperature Precision ± 0,05 C Temperature Accuracy ± 0,001 C Cell Type 2 SS electrodes, operating pressure 72 psi with ACS, 115 without Internal Cell Volume 0,8 µl Analog Output 1V /FS Digital Interface RS232c Autoranging - Autoscale Yes, User diseblable Gain From 1 to X 999 Weight approx 15 Kg Dimensioni (LxHxD): 320x165x260 mm
13 Ultrafluor 1200 Fluorescence Detector Whether you choose the handheld instant pilot, with extended data analysis capabilities, the 1200 Series fluorescence detector is easy to set up, use and maintain: Automatic optimization of PMT gain setting for high sensitivity and maximum linear range. Timetable setup of signal parameters, as well as excitation and emission scan ranges for maximum selectivity and sensitivity. Offline fluorescence scan provides complete sample characterization, for both excitation and emission, within a few minutes. Built-in diagnostic tools minimize downtime and increase operator safety. Superior sensitivity for lower detection limits at any combination of excitation and emission wavelengths. A long-life flash lamp, simplified optics and a reference system improve baseline stability. Improved selectivity and sensitivity by simultaneous multiple wavelength detection. Monochromators work in signal or spectral mode and are synchronized with the flash light to allow for detection at up to 4 excitation or emission wavelengths. Method optimization or peak confirmation by exploring the spectral landscape. View online spectra without interrupting your chromatography. Verify wavelength accuracy for GLP compliance - Automatically check the built-in wavelength calibration (Raman band H20) with external standards that your wavelength is indeed what the setpoint says it is. Built-in diagnostics software and long-life lamp.4000hrs. Set up analyses using simple dialogs with the instant pilot The instant pilot features all method parameters, timetable and diagnostics plus display of chromatograms and spectra. USB memory sticks are used to transfer methods between instruments.
14 Specifications 1200 Series Fluorescence Detector Performance Light source Pulse frequency Excitation monochromator Emission monochromator Photomultiplier Reference system Time programing Spectral acquisition Step size Wavelength repeatability Wavelength accuracy Flow cells Control and data evaluation Single wavelength operation: 10 fg anthracene LOD, Ex 250 nm, Em 400 nm H20 >500 Raman, Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell Dual wavelenght operation: H20 > 300 Raman, Ex 350 nm, Em 397 nm and Ex 350, em 450 nm, standard flow cell 20 W Xenon flash lamp, normal mode: 20 W economy mode: 5 W 365 Hz for signal mode, 73 Hz for economy mode Concave holographic grating, F/1.6, blaze 300 nm, range nm and zero-order, bandwidth 15 nm Concave holographic grating, F/1.6, blaze 400 nm, range nm and zero-order, bandwidth 15 nm nm, exchangeable Inline excitation measurement Up to four signals, response time, PMT gain, baseline behavior (append, free, zero), spectral parameters Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum nm, 10 nm step 1-20 nm ±0.2 nm ±2 nm Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz. EZChrom or Instant pilot control module without spectral data analysis UV-104M Dual Lamp Spectrophotometric Detector Is a detector with continuously variable wavelength in the range of nm and a noise level ± 0.5 x 10-5 AU The unit may be used for routine analytical chromatography as well as for preparative chromatography, according to the selected cell The unit is easy to operate, reference and sample signals are available for detector diagnostics as well as information on lamp operating hours. Output signal is available in both digital and analogue form on the output connector. It is possible to control the unit using the keypad or an RS232 interface Connecting capillaries are led from the detector s right side panel. 14
15 The detector is equipped with a high standard deuterium lamp in a special socket that enables easy changing. For visible part of spectrum is used as secondary light source a tungsten lamp. Wavelength can be programmatically changed during the performance of an analysis. The detector performs automatic wavelength calibration after the lamp has been switched on. The instrument include a Self Diagnostic Procedure The instrument enables possibility to generate a scan during the run to get spectral informations A cell is included as a component of the detector. The detector has unique stabilization time (less than 3 minutes). Thecnical Characteristic Injectors Wavelength range Spectral half-width Accuracy of adjustment Reproducibility Measurement Range Light source Noise level at empty cell (240 nm, TC 1 s) Drift at empty cell (240 nm after 1 h) Materials in contact with mobile phase Time constant Programming Adjustable offset Output for integrator nm 5 nm ± 1 nm ± 0.5 nm 0,002-3,0 AUFS Deuterium discharge lamp and tungsten lamp ± 0,5 x 10-5 AU 1x 10-4 AU/hr PTFE; fused silica, stainless steel, Vespel, PEEK Fast, middle and slow 3 change of wavelength during analysis ± 50 mv 1 V/AU Interface RS 232 Power supply Power input Dimensions (W x H x D) Weight 100, 115 and 230, 50/ 60 Hz) 80 VA 220 x 170 x 450 mm 11 kg 15
16 Rheodyne Injection Module This is the regular 6 ways, 2 positions injection valve in PEEK or SS Maximum working temperature: C - Nominal Pressure MPa(psi) : 34.5 (5000) - Maximum Pressure, MPa (psi): 48.3 (7000) - Material : Version in Inert Polimer (Peek) - Volume door-door: 660 nl Software Chrom&Spech per Windows with USB Key Chrom&Spec system provides a capability to make a complete chromatography workstation for HPLC, GC, IC, and preparative chromatography, as well as capillary electrophoresis systems. Connected to any chromatographic instrument with an analog output signal, it is able to acquire, process, display, and analyze data. "Chrom&Spec" system is suitable for use in most chromatographic instruments with any type of the detector. The software is designed with Good Laboratory Practice (GLP) and Good Automated Laboratory Practice (GALP) standards in mind, so the system is well fitted to the research laboratory or industrial environment. This software is already CFR21 Part 11 Protocol compliance, thanks to advanced audit trails features and security options. The Chrom&Spec data acquisition system consists of data acquisition hardware and data processing software that operate on an IBM-compatible computer in a Microsoft Windows environment. Chrom&Spec provides a complete set of data processing tasks such as analog-to-digital conversion, data manipulation, noise filtration, integration, peak identification and quantification, as well as report output. 16
17 DATA PROCESSING: Filtration of noise by several user-defined filters Batch reprocessing of several chromatograms after data acquisition Peak deconvolution module for multi-channel chromatograms by factor analysis (optional) INTEGRATION: Automatic detection of up to 2000 chromatographic peaks with opportunity of tuning by integration events or manual integration Identification of up to 500 components of analyzed mixture Integration Events Manual Integration Options CONTROL: Support of run queues Version with built-in control and monitoring of several types of HPLC instruments is available CHROMATOGRAM DISPLAY: Continuous shifting and scaling of the displayed part while measuring Parallel display and working with up to 100 chromatograms User-controlled axes description and scales Various options for the labelling of peaks DATA STORAGE: Storage of compressed raw data to disk for further reprocessing Complete set of method parameters is attached to data METHOD FILES: Include data acquisition, calibration and processing parameters GLP logs for method and data QUANTIFICATION & REPORTING: 17
18 Up to 23 parameters can be calculated for every peak: Retention Time; Name; Area. Area% or Height, Height%; Normalized Concentration; Absolute Concentration; Relative Concentration; Retention Index ; Capacity Factor; Efficiency (TP, TP/m HETP); Asymmetry; etc. Support of both US and European Pharmacopaea formulas User-customized printout of results IMPORT/EXPORT: ASCII output with user-definable delimiter character Capability to start user program immediately after report file has been made Import and export of Raw Data Analytical Instruments Association's Andi (*.CDF) file import/export SECURITY: Operation with a system of passwords providing various limitations on access of a user to data and method files 18
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23 ACS Suppressor The working principle of the suppressor is to eliminate the Sodium cations from the eluent flow thanks to a selective ion-exchange process that occurs inside the suppression membrane between sodium and H+ presents in the regenerant solution, generated by chemical process applied to the inlet to the detector s flow cell. Mobile Phase Flow Acid Solution Cation Exchance Membrane Ion Exchange Capillar Na + H+ Suppressor Bottle 23
24 TECHNICAL SPECHS Regenerant Solution Range of Concentration EMCES 21 - PORTLAB M H 2 SO 4 Total Dead Volume 100 µl Working Flow Range ml/min Specific Conductivity of Standard Eluent (1.7 mmoli NaHCO 3 /1.8 mmoli Na 2 CO 3 ), no more than Applicable Power Maximum Reverse Pressure Dimensions, mm 0,5 2,2 ml/min 40 µs/cm ma 5 bars 320х265х260 Mass, Kg Approx PCI driver Board 8 channels integrated inside PC This board, installed inside PC, allows connection and control of all component of the system thorugh the Sofwtare Chrom&Spech. CP-168U is a Smart Universal PCI Multiport Serial Board designed for POS and ATM applications, and for use by industrial automation system manufacturers and system integrators. CP-168U is compatible with all popular operating systems, and each of its 8 RS-232 serial ports supports data rates of up to Kbps, and provides full modem control signals, ensuring compatibility with a wide range of serial peripherals. In addition, CP-168U works with both 3.3V and 5V PCI buses, allowing the board to be installed in virtually any available PC server. TECHNICAL SPECIFICATIONS Hardware Connector Communication Controller DB62 Female MU860 (16C550C compatible) Interface Bus 32-bit, Universal PCI 24
25 No. of Ports Signals 8 (only one IRQ required) TxD, RxD, RTS, CTS, DTR, DSR, DCD, GND Performance Baud Rate 50 bps to Kbps Configuration Data Bits 5, 6, 7, 8 Stop Bits 1, 1.5, 2 IRQ, I/O Address Parity Flow Control BIOS assigned None, Even, Odd, Space, Mark RTS/CTS, XON/XOFF Operating Systems OS Supported Windows Vista (X86 & X64), Windows NT/2000/XP/2003, Windows 95/98/ME, Linux 2.6/2.4(Red Hat, SuSe, Etc.), Embedded Windows XP, QNX,DOS, FreeBSD, SCO Unixware 2.1,SCO UnixWare 7, SCO Openserver Power and Environment Power Requirements Operating Temperature Operating Humidity Storage Temperature 180 ma (+5V) 0 to 55 C (32 to 131 F) 5 to 95% RH -20 to 85 C (-4 to 185 F) Dimensions 120 x 82 mm (4.72 x 3.23in)(W x D) Surge Protection Regulatory Approvals Warranty Embedded 15 KV ESD Surge Protection EN55022 Class B, EN55024, EN , EN , FCC Part 15 Class B 5 years 25
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