Radnoti. Radnoti. Radnoti. Precise digitally controlled temperature. Illuminated chamber improves sample viewing
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1 Radnoti Myography Systems Precise digitally controlled temperature Illuminated chamber improves sample viewing Featuring an ergonomically designed sliding wrist rest Modular designed allowing for easy reconfiguration Radnoti Four Channel Myography System 4 Radnoti Single Channel Myography System 1 Designed for researchers performing contractile force studies on small ring samples with sizes ranging from 60 um to over 1 mm diameter that include mouse aortic rings and small intestinal ring samples down to micro-vessel preparation, such as mesenteric arteries. Radnoti 2007
2 no Radnoti 4 Four Channel Myography System Radnoti M series Myograph systems and components are true to the commitment of excellence and quality found in all Radnoti products. Available as the M 4 four channel and M 1 single channel Myograph system configurations. Radnoti 1 Single Channel Myography System
3 Precision Fabrication Using precision fabricated micro vessel support brackets, the system accommodates lumen diameters down to 60μm and segment lengths up to 3mm. For samples with lumen diameters of 1mm or more, the standard Radnoti Tissue Organ Bath systems are recommended. To mount the vessel segment, two individual parallel wires are passed through the sample lumen and attached to the micro vessel support brackets. Each vessel support bracket incorporates a micro groove on the Radno face to aid in maintaining the wire position when mounting. One bracket is connected to a precision 0 0.5gm capacitance type isometric force transducer (switchable to 0-5 gm and also available in 0-2 gm/0-20 gm configurations) that provides a true isometric measurement, i.e. less than 12.5μm deflection at 0.5gm force, which is critical when working with small vessel samples. The opposing Transducer Safety Lock An added feature is a safety lock for the ultra sensitive force transducer. The researcher is able to disable the movement of the micro vessel support bracket in a manner that protects the transducer. support bracket is connected to a precision micrometer controlling the X axis movement, with Y and minor Z axis positioning controls conveniently positioned on the Myograph chamber, allowing for precise alignment of the micro vessel support brackets. The wires are then anchored via a locking screw on each of the brackets. The locking screws feature a small bushing that is incorporated under the screw head greatly improving capture of the support wire. A pre-load tension is then applied via adjustment of the X axis micrometer. The researcher is able to disable the movement of the micro vessel support bracket in a manner that protects the transducer. The researcher is able to focus on mounting the sample with less worry about damaging the mechanics of the system.
4 Radno Lighted Chamber The sample chamber is illuminated with an imbedded light source in the wrist rest. The light is passed into the chamber via a glass light pipe integral to each of the individual chambers. When the chamber is docked into the wrist rest the light source aligns with the light pipe and light is passed to the chamber. The light source is activated via an on/off switch placed in the side of the wrist rest. When not in use for mounting, the user simply turns off the light source. The light source is powered by four AA batteries (included) that can be easily replaced when required. 5ml Working Volume Chamber Once mounted the sample is immersed into a 5ml working volume sample chamber that allows for filling, emptying and gassing of buffer solutions. A water-jacketed reservoir with a separate support stand is provided to permit convenient perfusion buffer exchanges. The elevation of the water jacketed reservoir provides a nominal pressure feed to the chambers simplifying fill cycles. Included is a water jacketed bath that can be used during the initial gross dissection that prepares the tissue segment for mounting. A vacuum flask is also provided with an appropriate manifold system to aid in empty cycles.
5 Precise Temperature Control The sample chamber is controlled to maintain accurate temperature of the sample and the buffer via the included M series thermostatic control unit. The control unit is available in both a four channel and single channel model. The M series thermostatic controllers precise electronics employ a adno communication loop from the microprocessor and the thermistor/ sensor. A variable electrical current is supplied to each individual chambers heating element in response to the thermistor probe signal. The probe is located directly in the sample chamber to avoid temperature over shoot greatly reducing the chance of overheating and tissue damage. Both M Series model thermostatic controllers incorporate a user friendly front panel alpha numeric LCD screen with a three button interface. The user steps through settings moving a blinking cursor using the up arrow or down arrow buttons. Ergonomically Designed for Ease of Use Both the single chamber base and the 4-chamber base assembly are ergonomically designed to allow the users wrist to be supported fully while in the process of manipulating the sample. This feature provides exceptional stability during the critical tissue mounting phase of the procedure. The units also allow for a low temperature and high temperature alarm setting. Once set, the controller continually monitors the individual vessel chambers temperature and displays the chambers value on the corresponding LCD screen. Temperature readings can be output via the rear panel RS-232 port for digital recording. Each vessel chamber uses easy to clean and replace buffer inlet, gas inlet and buffer outlet tubes. The tubes are fabricated of borosilicate glass to give the researcher a clear view of the fluid path during setup and experimentation.
6 o Sliding Wrist Rest The M4 four channel system has the added feature of a moving ergonomic wrist rest. This allows the user to move from chamber to chamber. Once the vessel is properly set the user simply moves the chamber unit back and moves on to the next chamber. The chambers with the loaded samples are then undisturbed during mounting of additional samples. This feature allows for easy manipulation of the chambers and helps steady the hands during use.
7 Radnoti M Series Myograph Chamber Buffer inlet tube Buffer outlet tube Gas inlet tube (sintered glass tipped) Transducer housing Micro vessel support bracket (fixed) Micro vessel support bracket (adjustable) Thermistor 5ml vessel chamber Y axis adjustment Z axis adjustment (minor) X axis adjustment micrometer
8 Radnoti M Series Myograph Chamber Micro vessel chamber light pipe Transducer safety lock Transducer amplifier connector Temperature controller connector
9 Myograph Material Specifications: Myograph chamber body: Myograph Base: Myograph Support Brackets: Support Bracket Linkage: Gas Inlet Tube: Buffer Inlet Tube: Buffer Outlet Tube: Polyester Material PET (Polyester) Opaque White Lowest Temperature -99 to -1 F Highest Temperature +201 to +300 F Operating Temperature Range -30 to +230 F Specifications met: (FDA) Compliant UL Rating: UL 94HB Thermostatic Temperature Controller Specifications: *PET Polyester Radno Chamber bath Temperature Control range: Ambient to 45C Regulation: +/- 1.0C Accuracy: +/- 1C Readout resolution: 0.1C Probe Sensor: Special LSI thermistor (YSI type 400 series) Display: 4-Line LCD. Temperature updated every second Power: 117v/60Hz or 220v/50Hz (as specified) Size: 22.5 x 7.5 x 17.5 cm (LxHxW) Weight: 1.25 kg Light Source: *PET Polyester *PET Polyester Stainless Steel Borosilicate Glass, Sintered Borosilicate Frit Borosilicate Glass Borosilicate Glass 4 AA Batteries Force Transducer Specifications: Isometric 0.0gm - 0.5gm / 0.0gm - 5.0gm force range (switch selectable) standard 0.0gm - 2.0gm / 0.0gm gm force range (switch selectable) optional Force Transducer Amplifier Specifications: Analog Electrical Output - 0 to 5.0 VDC depending upon the position of the range selector switch. *Output impedance is 2 kiloohms for direct connection to all recorders. Linearity is 0 to 100% overload (1 or 10 grams, depending on setting). Internal frequency response is selectable from 1000Hz to 10Hz
10 M 4 Myograph System Includes: 4 channel myograph base with sliding wrist rest and internal light source 1 Myograph chamber 4 Isometric force transducer and amplifier (transducer factory installed) 4 Myograph chamber calibration mount 1 M4 Series 4 channel Thermostatic Temperature Controller 1 Reservoir stand assembly 1 Radno Preparatory tissue bath 1 1 liter water jacketed reservoir 1 M4 Series tubbing kit 1 M4 Series cable set 1 M 1 Myograph System Includes: 1 channel myograph base with sliding wrist rest and internal light source 1 Myograph chamber 1 Isometric force transducer and amplifier (transducer factory installed) 1 Myograph chamber calibration mount 1 M1 Series 1 channel Thermostatic Temperature Controller 1 Reservoir stand assembly 1 1 liter water jacketed reservoir 1 M1 Series tubbing kit 1 M1 Series cable set 1 Preparatory tissue bath 1 Available Options: Olympus SZX10 Microscope Olympus SZ2-STU2 universal stand type 2 Myograph chamber covers Automated fluid control M Series Inverted microscope myograph chamber Accessories: Platinum field stimulating electrode set Wire myograph tissue strip mounting adapter set Optional Disecting Scope Data Acquisition: Speak to your local representative about optional hardware and software bundles, including data acquisition.
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