Mightex Simply Brighter. Polygon400 Multiwavelength Dynamic Spatial Illuminators (Part Numbers: DSI-x-xxxx-xxxx-xxx-xxx) PRODUCT DESCRIPTION FEATURES

Transcription

1 FEATURES Programmable image patterns Up to 4,000 frames per second 416K pixel DLP panel Wide range of available wavelengths Support LEDs or arc lamps Diffraction-limited projection High-throughput Etenduepreserving design External LED controller available for fast wavelength switching PRODUCT DESCRIPTION Spatially patterned illumination with temporal and spectral control enables numerous new techniques in life-science applications. For example, in optogenetics, selected neurons in a specimen slice can be activated or silenced with a user defined illumination pattern which leaves neighboring neurons unaffected. Polygon400 multi-wavelength dynamic spatial illuminator (DSI) integrates the state-of-theart spatial light modulators and high-power LEDs using a proprietary Etendue-preserving optical design to deliver high-intensity illumination patterns with superb resolution. A Texas Instruments DLP spatial light modulator is used to display a user defined image pattern. At the heart of the Polygon400 is a unique optical system that carefully delivers light from LED sources to the DLP panel and then through a microscope to the specimen plane. Such a systematic approach makes it possible to achieve maximum optical intensity while maintaining diffraction-limited imaging performance. Temporal performance is a key to many intended applications for Polygon400. With a frame rate of more than 4,000 fps and fast-switching LEDs, Polygon400 can deliver illumination patterns with micro-second precision. Using the Polygon400 with external LED controllers also enables wavelength switching in between illumination patterns at the highest frame rate. Fiber or lightguide input available Intuitive software for spatial, temporal and spectral control of illumination patterns APPLICATIONS Optogenetics Structured illumination Photoactivation Photostimulation (a) Polygon400 Dynamic spatial illuminator. (b) Left - Polygon400 beam combiner cube for upright microscopes; and, right - adapter ring for inverted microscopes. (a) Figure 1 - (a) Polygon400 installed on an inverted microscope; and (b) Polygon400 installed on an upright microscope. A dedicated software allows users to generate illumination patterns as well as control illumination intensity and timing. The software also supports calibration between illumination patterns and images acquired through any digital camera on a microscope. Polygon400 is designed to be easily inserted into the infinity path of a microscope. For inverted microscopes, the common point is usually the back port of the microscope where a fluorescence attachment is commonly placed. A filter cube is required to fold the DSI light path into the microscope. The filter cubes used for fluorescence observation serves this purpose well. For upright microscopes we can provide a beam combiner cube to be inserted below the binocular/ trinocular unit. The dichroic or mirror in the beam combiner directs the Polygon400 beam into the microscope light path. A C-Mount version is available as well for both upright and inverted microscopes. Customers may specify up to three LEDs in the range of 400nm to 700nm to be included in the built-in light source engine (E and I Series). A lightguide-coupled model Polygon400 (G Series) is also available where any light sources such as LEDs and arc lamps can be used for illumination. Note that the lightguide-coupled model does not have built-in LEDs. Both the E Series of Polygon400 bypasses the internal LED controller and allows users to directly control the built-in LEDs through external LED drivers. The cathodes and anodes of built-in LEDs are connected to a multi-pin connector on the back panel of Polygon400. External LED controllers enable several important features not supported by the internal LED controller. One can achieve higher dynamic range, higher intensity resolution(>10bits), higher peak current in pulse mode, and faster response(<10ns). The use of external LED controllers also makes it possible to control LED timing independent of the micromirror array. For example, while the micromirrors stay at a constant position, the LEDs can be modulated to generate desired intensity profile. On the other hand, when needed, strict synchronization between the LEDs and the micromirrors can be achieved via the external trigger signals provided by the Polygon400. supplies a variety of LED controllers that can be used with the E and G series of Polygon400. Software already includes synchronization features to support BLS- series LED controllers. For third-party LED controllers, synchronization can be implemented via trigger signals or Polygon400 SDK. (b)

2 PERFORMANCE SPECIFICATIONS Projection Area Projection area and pixel resolution (at 10X magnification) Common Microscopes Leica Nikon Olympus Zeiss Height (mm) Width (mm) Diagonal (mm) Pixel size ( m) To calculate illumination area and pixel resolution at the specimen, simply divide the above numbers by the magnification of the objective. For example, under a 10x objective on an Olympus microscope, the illumination area will be 0.78mm x 1.39mm with a pixel resolution of 1.62 m. Available LED Wavelengths and Output Power on the Specimen Under Microscope (E and I Series) Wavelength (nm) 1. Preliminary estimation and at specimen under a 20 x 0.75NA Olympus objective. Output Power 1 (mw) Control and Timing Parameters Minimum Maximum Unit Allowed bitmap depth 1 8 bit Exposure 1bit ,000 ms Frame 1bit - 4,000 1/sec Exposure 4bit ms Frame 4bit /sec Exposure 8bit ms Frame 8bit /sec Input trigger TTL, BNC connector - Input trigger delay - 50 s Output trigger TTL, BNC connector - Output trigger delay User Programmable ms System and communication USB2.0 interface. 5Vdc 3A input power. Windows XP Service Pack 2 or greater, Windows 7 and 8. Screen resolution of 1,366x768 or higher

3 INSTALLATION DRAWINGS (a) Installation drawing for I & E Series Polygon400 (with built-in LED s) (b) Installation drawing for G Series Polygon400 (with a lightguide adapter)

4 INSTALLATION DRAWINGS (Cont d) (d) Installation drawing for C Mount version of G Series Polygon400,

5 PART NUMBER AND ORDERING INFORMATION Series Part Number Description I E DSI-I-xxxx-yyy DSI-I-xxxx-xxxx-yyy DSI-I-xxxx-xxxx-xxxx-yyy DSI-E-xxxx-yyy DSI-E-xxxx-xxxx-yyy DSI-E-xxxx-xxxx-xxxx-yyy Polygon400 with built-in LED source(s) and internal LED controller. Polygon400 with built-in LED source(s). Works with external LED controller for more sophisticated control. G DSI-G-yyy Polygon400 for external, lightguide-coupled light sources. For lightguide with 5mm ferrule diameter and 15mm or greater ferrule length. Use the following part number configuration guide to build the Polygon400 Patterned Illuminator that best fits your application DSI Series Code I = I Series E = E Series G = G Series Wavelength Code (blank): external light source xxxx: one wavelength xxxx-xxxx: two wavelengths xxxx-xxxx-xxxx: three wavelengths Microscope Interface Format 000: Standard for infinite path 00C: C Mount to connect to camera ports LAPP: Nikon LAPP Compatible Examples 1. Polygon with three built-in LEDs (470nm, 530nm, 590nm) external LED controller, C Mount interface would be DSI-E C 2. A lightguide Coupled Polygon for standard infinity optics would be DSI-G-000 Wavelength Code Look-Up Chart for E & I Series Polygon Wavelength (nm) Wavelength Code For more information or assistance to put together a Polygon for your application requirements Please contact our solutions experts at or sales@mightex.com.