Leica TCS SPE. Spectacular Imaging! Technical Documentation
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1 Leica TCS SPE Spectacular Imaging! Technical Documentation
2 Leica TCS SPE Spectacular Imaging Easy to Achieve Built-in Reliability Affordable Excellence The new high resolution spectral confocal Leica TCS SPE is an integrated system for life science research. A reliable partner, providing spectacular results easily. A
3 B 0 C Research microscope A DM00 CSQ B DMI000 CSQ C DM00 CSQ Confocal scan head Supply unit, including or lasers and power supply 0 9 SuperZ Galvo stage Microscope control unit Camera port Digital camera EL000 fluorescence illumination control 9 Smart move microscope control 0 Computer table Anti-vibration microscope table Computer mouse Keyboard Monitor Workstation Control panel
4 Specifications Leica TCS SPE Scan Head Scanner Method True Confocal Point Scanner Confocal channels Scanner Galvo, [x,y] Sequential scan Channels, sequential multiplexing Resolution Range (min - max) [pixel] 0 Scan formats [pixel],,, 0, 0 Image depth [bit] or, switchable Spectral Detection Spectral detection Type continuously variable Spectral resolution [nm] nm Bandwidth [nm] 0-0 Detector Detector Detector type ultra high dynamic PMT Detector connection direct Illumination laser Pinhole Pinhole type motorized, variable Range (min - max) [µm] 00 Pinhole adjustment [%] 0 00 Control automated via GUI Beam Splitter Type high performance dichroics Beam splitter wavelength [nm] 0// (nm laser systems) 0/ Beam splitter wavelength [nm] 0/// (nm laser systems) / ND-splitter for TLD/λ [%] 0/0 Beam splitter exchange automated Zoom Zoom type continuously variable Zoom range x x Zoom increment 0. Scan Modes D xt D xyz; xyz Time xyt; xzt Lambda xyλ; xzλ Time xyzt; xzyt Time & lambda xyλt; xzλt Lambda xyλz Time & lambda xyzλt FOV Field of view (diagonal) [mm]. Speed Speed mode uni-, bi-directional Line speed range [Hz] 00, 00, 00, bi: 00, 00 bi-directional [f/s]. [f/s]. 0 [f/s] 0.
5 Supply Unit Laser Laser type solid state Laser max Laser excitation wavelength [nm] 0,,,, Excitation attenuation AOTF, direct modulation Excitation attenuation control automated Range [%] 0 00 Computer External PC Processor Intel Core i Memory [GB] HD-Size [GB] 00 Operating system Windows Interfaces USB FireWire (x 9a, x ) Ethernet Serial 0 Mouse optical Keyboard Monitor Graphics resolution [Pixel] 0 x 0, 0 x 00 (optional) Monitor x 9'', x 9'' (optional), 0'' (optional) Power Supply Power supply integration Type autoselect Voltage range [V] 00-0 Z-Drive Z-focus galvanometer stage Z-resolution 0 [nm] Z-focus device, other microscope drive DM00 Digital Cameras Support Leica DFC0 FX Digital Camera Leica DFC0 FX Digital Camera Leica Roper CoolSNAP HQ Camera Leica Hamamatsu Camera Microscope Types upright DM00 CSQ DM00 CSQ inverted DMI000 CSQ Software Export Formats LIF, TIFF, AVI, JPEG, MOV Modules LAS AF SPE Live Data Mode LAS AF SPE Colocalization LAS AF SPE Deconvolution LAS AF SPE D Visualization LAS AF SPE Dye Finder LAS AF SPE Core Review Software LAS AF SPE Camera Advanced LAS AF SPE FRAP LAS AF High Cont. Screening Autom. Environment Ratings Humidity [%] 0 0 Operating temperature [ C] 0 Guaranteed stability C +/- C
6 System Overview Leica TCS SPE Confocal Systems Confocal Scanning Units Solid State Laser: Scan Head Scanning Accessories: Microscope Acc R: SS mw nm Y: SS 0 mw nm G: SS 0 mw nm B: SS 0 mw nm V: SS mw 0 nm R Y B V Solid State Laser Supply Unit AOTF PC Fast Z Movement SuperZ Galvo Focus SPE Keyboard, Mouse SuperZ Galvo Inserts 9a Insert for SuperZ, standard Fluo Equipmen EL 000 Exter Li Ad Oc Confocal Prepared Microscopes 9 DM 00 CSQ VIS Lasersafe x fluo BF incl. Optical Outfit DM 00 CSQ VIS Lasersafe x fluo BF incl. Optical Outfit DMI 000 CSQ VIS Lasersafe x fluo BF incl. Optical Outfit Additional Options: Transmitted Light Detector-Kits 0 00 TLD for DM 0 0 TLD for DMI Optical Outfit DIC for Microscopes 9 Optical outfit DIC for DM 00 CSQ 9 Optical outfit DIC for DM 00 CSQ 9 Optical outfit DIC for DMI 000 CSQ DIC Prism Sets for Basic Objective Sets 9 9 DM00 Prism Set Basic 9 0 DM Prism Set Basic 9 0 DMI Prism Set Basic 9 DM00 Prism Set Basic V-VIS 9 DM Prism Set Basic V-VIS 9 DMI Prism Set Basic V-VIS Motorized xy-stage Kits 0 00 DM Basic -Plate Stage motorized kit 0 0 DM Basic -Plate Stage motorized kit upgr. 0 0 DMI Basic -Plate Stage motorized kit 0 0 DMI Basic -Plate Stage motorized kit upgr. Digital Camera Kit 00 Leica DFC0 FX Digital Camera Kit SPE 0 0 Leica DFC0 FX Digital Camera Kit SPE 0 0 Roper CoolSNAP HQ Camera Kit SPE 0 0 Hamamatsu Camera Kit SPE SuperZ Galvo Stage Inserts 9a Plate Inserts 0 0 Stage Insert for SuperZ, rotatable 9 Stage Insert for SuperZ, universal 0 Stage Insert for SuperZ, basic (system included) 0 0 Plate Insert -Microtiter Plate 0 Plate Insert -Willco Dish mm 0 Plate Insert -Willco Dish 0 mm 0 Plate Insert -MatTek Dish mm 0 Plate Insert -Petri dishes mm 0 Plate Insert -Petri dishes 9 mm
7 Confocal Systems RGB/RYB essories: t nal light source HXP0 burner quid light guide apter, Filter, ulars 0 0 Leica TCS SPE w. DM00 RGB 0 0 Leica TCS SPE w. DM00 RGB 0 0 Leica TCS SPE w. DMI000 RGB 0 0 Leica TCS SPE w. DM00 RYB 0 0 Leica TCS SPE w. DM00 RYB 0 0 Leica TCS SPE w. DMI000 RYB Confocal Systems RGBV/RYBV 0 0 Leica TCS SPE w. DM00 RGBV 0 0 Leica TCS SPE w. DM00 RGBV 0 0 Leica TCS SPE w. DMI000 RGBV HC-Objectives for RGB/RGBV Systems 900 Basic Objective Set HC 0x,0x, 0 HC PL APO 0x/0.0 CS 0 HC PL APO 0x/0.0 Imm/Corr CS 0 Leica TCS SPE w. DM00 RYBV 0 Leica TCS SPE w. DM00 RYBV 0 Leica TCS SPE w. DMI000 RYBV Confocal Prepared Microscope for all types including Microscope Accessories 0 HCX PL APO 0x/. Oil CS 0 HCX PL APO x/.0 Oil CS 09 HCX PL APO x/.0 W Corr CS Leica TCS SPE DM00 Confocal Prep. 0 0 Leica TCS SPE DM00 Confocal Prep. 0 0 Leica TCS SPE DMI000 Confocal Prep. Confocal Scanning Units 09 HCX PL APO x/.0 Glyc Corr CS ACS Objectives for RGBV/RYBV Systems 0 0 Leica TCS SPE Scanning Unit RGB 0 0 Leica TCS SPE Scanning Unit RYB 0 0 Leica TCS SPE Scanning Unit RGBV 0 Leica TCS SPE Scanning Unit RYBV 90 9 Basic ACS Objective Set 0x,0x,x 090 ACS APO 0x/0.0 Dry CS 090 ACS APO 0x/0,0 Imm CS 090 ACS APO 0x/. Oil CS 0900 ACS APO x/.0 Oil CS Climate Control Hardware-Options Software LAS AF - Screening TCS SPE HCS A High Content Screening Automation 0 0 Universal Gas Cover for SuperZ w. In/Outlet 0 Control Panel for TCS SPE LEICA HCS A for TCS SPE-II see LAS AF Matrix Products 9c 0 0 Second 9"-TFT Monitor for TCS SPE Software LAS AF - Imaging TCS SPE 0 00 TCS SPE CO Control Set 0V/0V 0 0 Eizo 0" high brilliance monitor TCS SPE 0 0 LAS AF SPE Live Data Mode 9c Including: SuperZ CO Cover 0 0 LAS AF SPE Co-Localisation CO Controller, Humidifier Tables 0 0 LAS AF SPE Deconvolution Climate Chamber for DMI 000 CSQ Including Heating & Temp Control Unit 9 90 Microscope Table for Upright and Inverted Systems, passive 0 90 Computer Table 0 0 LAS AF SPE D Visualisation 0 0 LAS AF SPE Dye Finder 0 LAS AF SPE Camera Advanced 0 LAS AF SPE FRAP
8 Room Requirements Power Supply Power supply integration Type autoselect Voltage range [V] 00-0 Power consumption [VA] 00 Independent circuits [no.] Frequency [Hz] 0/0 Fuse: standard [A] 0 visible radiation: Environment Humidity (noncondensing) [%] 0-0 Operating temperature [ C] - 0 Guaranteed stability C +/- C Load Capacity and Weight Confocal unit, max [kg] Microscope, max [kg] System [kg] 0 Static floor load [kg/m ] 0 LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
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