Supporting Information
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1 Supporting Information Quantitative Profiling of Oxidized Phospholipids in Lipoproteins from Patients with Coronary Artery Disease by Hollow Fiber Flow Field-Flow Fractionation and Nanoflow Liquid Chromatography-Tandem Mass Spectrometry Ju Yong Lee, Seul Kee Byeon, Myeong Hee Moon* Department of Chemistry Yonsei University Seoul, South Korea Table of Contents Operation of MxHF5. S-2 Experimental Conditions of nlc-esi-ms/ms S-3 Figure S1 S-4 Figure S2 S-5 Figure S3 S-6 Table S1 S-7 S-1
2 Operation of MxHF5 Operation of MxHF5 for the semi-preparative scale separation of HDL/LDL/VLDL from plasma samples began by injecting plasma into the injector with the dotted flow paths (injection followed by focusing/relaxation) in Figure 1. Sample components were delivered to the MxHF5 module with 1/10 of the pump flow, which was adjusted by the pressure loop attached to the 4-way valve. During this period, 9/10 of the pump flow was delivered to the outlet end of the MxHF5 so that the two converging flow streams could be focused at a position 1/10 th of each fiber length, while all flow streams exited through the fiber wall as a radial flow so as to provide sample relaxation. After a focusing/relaxation period that is dependent on the radial flow rate, both 3-way and 4-way valves were converted to solid line configuration so that the pump flow was directed to the channel inlet only for the beginning of separation. The second 3-way valve shown in the center of Figure 1 was employed to change the channel outflow rate from & into V & right after the elution of LDL so out, 2 nd Vout, 1 st that long-retaining VLDL could be eluted at an increased outflow rate. Flow rate control for each outlet of the 3-way valve was performed by varying the length of the capillary tube. S-2
3 Experimental Conditions of nlc-esi-ms/ms Figure S1. System configuration of nanoflowlc-esi-ms-ms used for profiling phospholipids, including the lyso- and oxidized forms. For preparation of the capillary column, one end of the empty capillary was sharply pulled out with a flame. The other end of the capillary was immersed to a slurry made by mixing MeOH with C18 beads, and the mixture was used to pack the empty capillary using high pressure N 2 gas. For the analytical column, a Pt wire to support electric power for ESI, pump flow, and the on-off valve was connected to the PEEK microcross as shown in Figure S1. During sample loading from the autosampler to the column, the pump flow with the mobile phase A (10:90 (v/v) CH 3 CN:H 2 O) was delivered at 600 nl/min for 10 min with the on-off valve closed. After loading, the pump flow was raised to 10 µl/min in order to reduce dwell time, and the on-off valve was simultaneously turned on so that the high speed pump flow could be split at the microcross to deliver only 300 nl/min into the analytical column. In order to prevent packing materials in the analytical column from spilling backward during the change of the on-off valve, a high pressure in-line filter unit containing a PEEK frit from IDEX Health & S-3
4 Science Co. was positioned between the microcross and the C18 capillary column as shown in Figure S1. During LC run, a binary gradient separation was applied by increasing the composition of mobile phase B (20:20:60 (v/v/v) CH 3 CN:CH 3 OH:isopropanol). Ionization modifiers were added to both mobile phases: 0.05% ammonium hydroxide in the negative ion mode of the LC run and 0.1% formic acid in the positive ion mode. Gradient elution conditions for the LC run in the negative ion mode of MS were as follows: the mobile phase B was increased to 60% in 1 min, to 80% in 21 min, to 100% in 1 min, and then maintained at 100% for 40 min for the LC run at negative ion mode. In positive ion mode, the mobile phase B was ramped to 80% in 1 min, to 100% in 1 min, and then maintained at 100% over 40 min. Injection amounts of extracted lipids from the lipoprotein fraction were 5 µg in the negative ion mode and 10 µg in the positive ion mode. Run conditions for MS operation during nlc-esi-ms/ms were as follows: ESI voltages were 2.0 kv in the negative ion mode (-) and 3.0 kv in the positive ion mode (+), the mass ranges for the precursor scan were amu (-) and amu (+), the normalized collision energies were 40% (-) and 45% (+), and the isolation widths for CID were 1.5 (-) and 1.0 (+). S-4
5 Figure S2. Base peak chromatograms of the PL standards (2 pmol each) at a) negative mode and b) positive mode by nlc-esi-ms/ms, which are the same run conditions for the plasma lipid extracts from each lipoprotein fraction obtained by MxHF a. negative ion mode MS Intensity 2b 1b 2a 1a :0-LPA 2. 14:0-LPG 3. 12:0/12:0-PA 4. 18:0-LPG 5. 12:0/12:0-PG 6. 16:0/16:0-PA 7. 16:0/18:2-PI 8. 18:0/18:0-PG % of solvent B MS Intensity Time (min.) 3 4 b. positive ion mode :0-LPC 2. 14:0-LPE 3. 12:0-PC 4. 14:0-PE 5. 14:0/16:0-PC 6. 18:0/22:6-PE % of Solvent B Time (min.) S-5
6 Figure S3. BPCs of lipid extracts from each lipoprotein fraction from the pooled CAD patient plasma sample obtained at a) negative ion mode and b) positive ion mode by nlc-esi-ms/ms. a. negative ion mode b. positive ion mode HDL HDL MS Intensity LDL MS Intensity LDL VLDL VLDL Time (min) Time (min.) S-6
7 Table S1. Identified PLs and Ox-PLs from each lipoprotein class besides PI class. Each species detected in pooled control (C) and patients (P) sample is checked with O. PA C/P C/P C/P C/P C/P C/P :0 16:0 o/o o/o o/o lyso 18:0 o/o o/o o/o :0 16:1 o/o :0 lyso o/ /o /o :0 18:1 o/o o/o o/o :1 lyso o/o o/o /o :0 18:2 o/o o/o o/o lyso 18:2 o/ o/o o/o :0 20:0 o/o /o o/o :2 lyso /o :0 20:3 /o o/o o/o lyso 18:3 /o :0 20:4 o/o o/o o/o :3 lyso /o /o :0 20:5 o/o /o /o :0 lyso o/ :0 22:1 o/o o/ :1 lyso /o :0 22:2 o/o o/o :2 lyso o/o :0 22:3 o/ lyso 20:3 /o :0 22:5 o/ o/o o/ :3 lyso /o :0 22:6 o/o o/o o/o :4 lyso o/o o/o o/o :0 24:3 o/ o/ :5 lyso /o :0 24:4 o/o o/ o/o :6 lyso o/ o/ o/o :1 18:1 o/ :0 10:3CHO o/o /o :1 18:2 o/ o/ /o :0 10:3COOH+O /o :0 18:0 o/o o/o o/o :0 11:0CHO /o :0 18:1 o/ o/ o/ :0 11:2CHO /o :0 18:2 o/o o/o o/o :0 18:1+O /o :0 20:0 o/ o/ o/o :0 18:2+O o/o o/o o/o :0 20:3 o/ o/ :0 20:3+2O o/o o/ :0 20:4 o/o /o :0 20:3+OO /o /o :0 22:1 o/ :0 20:4+2O o/o o/ :0 22:2 o/ o/o :0 20:4+O /o o/o o/o :0 22:3 o/ :0 20:5+2O /o :0 22:5 o/o /o o/o :0 20:5+O o/o /o :0 22:6 o/o o/o o/o :0 22:5+2O o/ o/ :1 18:1 o/ /o o/o :0 22:5+OO /o :1 20:2 /o :0 22:5+O o/o :1 22:6 o/o o/o o/o :0 22:6+2O o/ o/ o/ :2 18:1 o/o o/ :0 22:6+O o/ o/o o/o :2 18:2 o/o o/o :0 6:5CHO o/ :2 22:6 o/ :0 10:3CHO o/o /o :1 20:4 o/o o/ :0 18:2+O o/o o/o o/o S-7
8 :1 22:6 /o o/o :0 20:3+2O o/ :4 18:1 /o :0 20:4+2O /o /o :4 20:0 o/o /o o/ :0 20:4+O o/o o/o o/o :5 18:0 o/o o/o /o :0 21:5CHO o/ :5 20:1 o/ :0 22:5+O /o /o :4 18:0 o/ :0 22:6+O o/ /o /o :5 20:0 o/ :0 6:0COOH /o :5 20:1 o/ :1 10:3CHO o/o o/o o/o :6 20:0 o/o o/ :1 18:2+O o/o o/o o/o lyso 14:0 o/ o/o /o :2 10:3CHO /o o/ :0 lyso o/o o/o o/o :2 18:2+O o/o lyso 16:0 /o :2 7:1CHO o/ :0 lyso o/o o/o o/o :2+O 18:1 o/ lyso 16:1 o/ :2+O 18:2 o/o :3 lyso o/ /o /o PG C/P C/P C/P C/P C/P C/P :0 16:0 o/ o/o :2 16:0 /o :0 20:1 o/ :6 16:0 o/o /o :1 16:0 /o :0 lyso o/o o/o :0 16:0 /o o/o o/ :0 lyso /o o/o o/o :0 18:0 /o o/o lyso 16:3 o/ :1 16:0 o/o o/o o/o lyso 18:0 o/o o/o :1 16:1 o/o o/ :0 lyso o/o /o o/o :1 18:0 o/ :1 lyso /o o/o :1 18:1 /o o/o /o :2 lyso /o :1 18:2 o/o o/o o/o :5 lyso /o :2 16:0 o/o o/o o/o :2+O 18:0 o/ /o :2 16:1 o/o o/o :2+OO 18:0 /o :2 18:0 o/o /o :2+O 16:0 o/o o/o o/o :2 18:2 o/ o/ o/ :3+O 16:0 o/o /o :3 16:0 o/o o/o /o :3+OO 16:0 /o /o :1 16:1 o/o o/ o/ :6+O 16:0 /o :1 18:3 o/ :6+2O 16:0 /o PE S-8
9 C/P C/P C/P C/P C/P C/P :0 18:2 /o /o :0 lyso o/o o/o /o :0 20:5 o/o o/ :1 lyso /o o/ /o :1 20:4 o/o o/o :0 lyso /o o/o /o :1 18:1 /o o/o lyso 18:1 /o o/ /o :1 18:2 o/o o/o o/o :1 lyso /o o/o /o :1 20:3 o/o o/o o/ :2 lyso o/o :2 20:0 /o o/ :3 lyso o/o o/o :1 20:4 o/ :4 lyso o/o o/o :6 18:0 o/ o/o /o lyso 20:6 /o /o :6 18:1 /o :6 lyso o/o /o :0 16:0 o/o /o :1 18:2+O o/o o/o :1 16:0 o/o /o :0 20:5+O o/o :0 22:6 o/o o/o :6 18:0+O /o o/o /o :6 18:0 o/o o/o o/o :6 18:0+OO /o /o :0 16:0 o/ PC C/P C/P C/P C/P C/P C/P :0 18:0 /o lyso 16:0 /o /o :0 18:1 o/ o/o :0 lyso /o o/o /o :0 14:0 o/ :1 lyso /o o/o /o :0 16:0 o/o o/o o/o lyso 18:0 o/ o/ :0 16:1 o/ /o :0 lyso o/o o/ o/ :0 18:1 o/o o/o o/o lyso 18:1 /o /o o/o :0 18:2 o/o o/o o/o :1 lyso o/o o/o o/o :0 18:3 o/ o/o o/ lyso 18:2 /o o/o o/o :0 20:2 o/o o/o :2 lyso o/o o/o o/o :0 20:3 o/o o/o /o lyso 18:3 o/ o/o o/o :0 20:4 o/o o/ :3 lyso o/o /o :0 20:5 o/o o/o o/o :1 lyso /o o/o :0 22:3 o/ lyso 20:2 o/o :0 22:5 /o :2 lyso o/o o/o /o :1 16:1 o/ /o o/ lyso 20:3 o/ :1 18:1 o/o o/o o/o :3 lyso /o o/o :1 18:2 o/ o/ /o :4 lyso o/o o/o /o :1 22:5 o/o /o lyso 20:5 o/ /o :0 16:0 o/o o/o o/ :5 lyso /o o/o /o :0 18:0 o/o o/o lyso 20:6 o/ S-9
10 :0 18:1 o/o o/o o/o :6 lyso o/ o/o :0 18:2 o/o o/o /o :3 lyso o/ :0 18:3 o/o o/o /o :4 lyso o/ :0 20:3 o/o o/o o/o :5 lyso o/o /o :0 20:4 o/o o/o o/o :6 lyso o/o o/o :0 20:5 o/o o/o o/o :0 8:2CHO /o :0 22:4 o/o o/o :0CHO 16:0 o/ :0 22:6 o/o o/o o/o :0 9:0CHO o/o /o o/ :1 14:0 o/ :0 10:1CHO o/o /o /o :1 18:1 o/o o/ o/o :0 8:0CHO /o :1 18:2 o/o o/o o/o :0 8:1CHO+OO /o :1 20:1 o/o :0 9:2CHO o/o :1 20:4 o/o o/ /o :0 11:1CHO o/o o/o :2 14:0 o/o :0 9:0CHO /o /o o/ :2 18:2 /o :0COOH 16:0 o/ :2 18:3 o/ o/ :3CHO 16:0 /o /o :2 22:0 o/ :1CHO 16:0 o/ :2 22:6 o/ /o :0 10:1CHO /o :3 14:0 o/ /o o/ :0CHO 16:0 o/o :3 16:0 o/o o/o o/o :0 9:0COOH /o /o :3 16:1 o/ o/ o/ :3CHO 18:0 /o :3 18:1 o/ /o /o :0 11:1CHO /o :3 18:2 o/o :0COOH 16:0 /o :4 16:0 o/ :4CHO 16:0 /o /o :0 18:2 o/ :0 14:3CHO o/ :1 16:0 /o /o /o :0 12:3CHO o/o :2 18:0 o/ o/o :0 12:2CHO /o /o o/o :4 14:0 o/o o/o /o :0CHO 16:0 o/o :4 16:1 o/ o/ :5CHO 16:0 /o /o :5 14:0 o/o o/ :2 16:1CHO o/ /o :5 16:1 /o /o :0 18:3+O o/ o/o o/ :5 18:2 /o o/ :1+O 18:2 o/o /o :6 16:0 o/o o/o o/o :0 18:2+O o/o o/o /o :6 18:0 o/ /o :0 18:1+O /o o/o :0 16:0 o/ :0 17:0COOH /o /o :1 18:2 o/ /o :0 20:5+O o/o /o :2 16:0 o/ :0 18:3+O o/o o/o /o :4 16:0 /o o/o o/ :1+O 16:0 /o /o /o :5 18:0 o/o /o /o :0 18:2+O&OO o/ :5 18:1 o/ /o :0 20:5+OO /o /o o/ :6 14:0 /o /o :1+OO 16:0 /o /o S-10
11 :6 16:0 o/o o/o o/o :0 20:5+O /o :6 16:1 o/ o/o :4+O 16:0 /o o/o :6 18:1 o/o o/ o/o :0 18:3+OO&O /o :5 18:0 o/ o/o o/ :5+O 18:0 o/o /o :0 lyso /o /o S-11
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