Guard Columns ---- HPLC Column Catalog

Transcription

1 Guard ---- Guard s selection and Use 092 Cartridge Guard E 093 Cartridge Guard Ei (Non-metal type) 094 Guard for UHPLC 096 GL Cart 098 Packed Guard, Mini Guard 099 SILFILTER TM STD C18 4 Filters, Impurity Remove 5 HPLC Catalog 091

2 Guard s Selection and Use Guard columns are installed between the injector and the analytical column of a HPLC system, mainly to protect analytical columns. Guard columns are widely used as a cost effective for prolong HPLC column life. Exchangeable cartridge design and unchangeable packing design are offered. Exchangeable cartridge design contains holder and cartridge column, and the cartridge columns are disposable, easy -to-use, and you can change the guard column in seconds. Packed design is packed as same as analytical column, using as a guard column. Guard column design Description Compatible analytical columns ID Guard columns length Cartridge Guard E Page 93 First choice for Guard 1.0~1.5 mm mm Cartridge Guard Ei Page 94 Guard column with PEEK inlet and outlet 2.1~4.6 mm, 20 mm Exchangeable Cartridge design UHPLC Guard Page 96 For high speed, high efficiency separations 1.0~3.0 mm mm SILFILTER STD C18 Page 4 Almightily for various ODS columns 3.0~4.6 mm mm GL-Cart Guard Page 98 Direct-Connection and Indirect- Connection designs with economic cost 4.0, 4.6 mm 5 mm Packed Guard Page 99 Packed as same as analytical column 1.0~4.6 mm 33, 50 mm Unchangeable packed design Packed Mini Guard Page 3 Short length type of packed guard column 4.0, 4.6 mm mm Preparative Guard Page 114 Guard columns for preparative columns 6.0~0 mm 50 mm 75 mm(for 50 mm ) 0 mm (for 0 mm ) Selecting packing material When selecting the packing material for the guard columns, we recommend to choose the same with analytical columns. However, SILFILTER STD C18 is an monolithic guard column, it can fi t different kinds of ODS columns. Selecting dimensions Particle size : To not alter the chromatography of the analytical column, the packing of the guard column should be the same as the analytical column. Inner diameter : Choose a guard column with an internal diameter similar to the analytical column internal diameter. Pre-column Coupler W (PCTFE) Connectors Universal self-adjusting connector Coupler, using for when connect guard columns and analytical column. Pre-column Coupler SUS 092 HPLC Catalog

3 Cartridge Guard E Cartridge guard column E designed with an special holder and cartridge, universal selfadjusting connections, reusable by changing cartridges. Cartridges contain the same high performance packing material that are used in our GL Sciences analytical columns. Easy-to-use, convenient, and disposable guard columns can protect your more expensive analytical columns. Reversed Phase Line-up list InertSustain C18 of the Length E Guard Cartridge E Holder/ (2 Cartridge E Guard 3 μm 5 μm 3 μm 5 μm , , , , , Inertsil ODS-4 of the Length E Guard Cartridge E Holder/ (2 Cartridge E Guard 3 μm 5 μm 3 μm 5 μm , , , , , Inertsil ODS-3 of the Length E Guard Cartridge E Holder/ (2 Cartridge E Guard 3 μm 4 μm 5 μm 3 μm 4 μm 5 μm , , , , , Inertsil ODS-2 Cartridge Guard E of the Length E Guard Cartridge E Holder/ (2 Cartridge E Guard , , , , , * Other packing material of cartridge guard columns E are available, please confi rm page 5~87 of each analytical columns. HILIC Normal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. No. Index HPLC Catalog 093

4 Cartridge Guard Ei (Non-metal type) Cartridge Guard column Ei is a special design for the separation which need to avoid the metal accessible surface. It is signed with a special PEEK holder and cartridge. * Length of Cartridge Guard column Ei is mm Cartridge Guard Ei (Non-metal type) InertSustain C18 of the 1.0, , Inertsil ODS-3 of the 1.0, , Inertsil ODS-SP of the 1.0, , Inertsil ODS-EP of the 1.0, , Inertsil C8-4 of the 1.0, , Inertsil ODS-4 of the 1.0, , Inertsil ODS-2 of the 1.0, , Inertsil ODS-P of the 1.0, , InertSustain C8 of the 1.0, , Inertsil C8-3 of the 1.0, , * All Cartridge Guard column Ei designed with mm length. 094 HPLC Catalog

5 Cartridge Guard Ei (Non-metal type) InertSustain Phenyl of the 1.0, , Inertsil CN-3 of the 1.0, , Inertsil Amide of the 1.0, , InertSustain NH2 of the 1.0, , Inertsil Diol of the 1.0, , Inertsil WP300 Diol of the 1.0, , Inertsil Ph-3 of the 1.0, , Inertsil WP300 C18 of the 1.0, , Inertsil HILIC of the 1.0, , Inertsil NH2 of the 1.0, , Inertsil SIL-0A of the 1.0, , Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. No. Index * All Cartridge Guard column Ei designed with mm length. * Other packing material of cartridge guard columns Ei are available, please contact your local distributor for more information. HPLC Catalog 095

6 Guard for UHPLC Holder for UHPLC Guard Guard InertSustain C18 Length Guide Packing Guard column for UHPLC signifi cantly extends column lifetime of your analytical column. The replacement cartridge guard column can be exchanged easily. A 1/16 inch tube is connected at the outlet of the holder for easy installation with minimized dead volume. Specifications Silica : High Purity Silica Gel : 2 μm, 3 μm Maximum Pressure : 80 MPa column : 1.0 ~ 3.0 mm Guard (2 EA) 2 μm 3 μm 2 μm 3 μm InertSustainSwift TM C18 Guard (2 EA) Length 1.9 μm 3 μm 1.9 μm 3 μm Inertsil ODS-4 Length Guard (2 EA) 2 μm 3 μm 2 μm 3 μm Inertsil ODS-3 Length Guard (2 EA) 2 μm 3 μm 2 μm 3 μm Inertsil ODS-SP Length Guard (2 EA) 3 μm 3 μm HPLC Catalog

7 Guard for UHPLC InertSustain C8 Guard (2 EA) Length 2 μm 3 μm 2 μm 3 μm Inertsil C8-4 Guard (2 EA) Length 2 μm 3 μm 2 μm 3 μm Reversed Phase HILIC Normal Phase Inertsil C8-3 Guard (2 EA) Length 2 μm 3 μm 2 μm 3 μm InertSustain Phenyl Guard (2 EA) Length 2 μm 3 μm 2 μm 3 μm SEC Ion Exchange Application Specifi c Inertsil Ph-3 Length Holder Guard (2 EA) 2 μm 3 μm 2 μm 3 μm Description Cat.No. Holder for UHPLC Guard * The tube size at the holder is 1/16 inch O.D mm 30 mm length. Installation Outlet side of the injector 1/16 inch Female (No.-32UNF) Tubing tip length is 2.4 mm. side Purchase a proper joint. Joint Very High Pressure Re-useable Fitting Description Max. Pressure QTY Cat.No. VHP MPa 1 set VHP MPa 1 set * The tube size at the holder is 1/16 inch O.D mm 30 mm length. VHP-300 VHP-320 Guard Preparative Capillary Applications Cat. No. Index HPLC Catalog 097

8 GL Cart GL Cart guard column are designed as economical type to protect your expensive analytical column. GL Cart is one dimension 5mmL 4.6mm (Compatible analytical columns ID is 4.0 mm, 4.6 mm.) GL Cart holder is reusable. InertSustain C18 Inertsil ODS-4 Packing material Particle size (μm) Cartridge guard column GL Cart EA.(5 4.6 mm ) Cat. No. GL Cart Set (5 GL Cart cartridge + 1 holder ) Cat. No Inertsil ODS Inertsil ODS Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP InertSustain C8 Inertsil C8-4 Inertsil C8-3 InertSustain Phenyl Inertsil Ph Inertsil CN Inertsil WP300 C Inertsil WP300 C Inertsil WP300 C Inertsil Amide Inertsil HILIC InertSustain NH2 Inertsil NH2 Inertsil Diol GL Cart Inertsil SIL-0A Inertsil SIL-150A Inertsil WP300 SIL Inertsil WP300 Diol * Other packing material of GL Cart guard columns are available, please contact your local distributor for more information. 098 HPLC Catalog

9 Packed Guard, Packed Mini Guard Packed guard column and Mini guard column are designed different with cartridges type, using a high pressure to pack it as a guard column, and also can sustain analytical column s performance. Packed guard column has length 33 mm and 50 mm two types, and Mini guard column has length mm. Reversed Phase Packed Guard Packed Mini Guard Packed Guard InertSustain C18 Inertsil ODS-4 Inertsil ODS-3 Inertsil ODS-2 Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP Packing material Length 33 mm Length 50 mm 3 μm 5 μm 3 μm 5 μm HILIC Normal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. No. Index HPLC Catalog 099

10 Packed Guard, Packed Mini Guard Packed Guard columns InertSustain C8 Inertsil C8-4 Inertsil C8-3 Inertsil C8 Inertsil C4 InertSustain Phenyl Inertsil Ph-3 Inertsil Ph Packing material Length 33 mm Length 50 mm 3 μm 5 μm 3 μm 5 μm HPLC Catalog

11 Packed Guard, Packed Mini Guard Packed Guard columns Inertsil CN-3 Inertsil WP300 C18 Inertsil WP300 C8 Inertsil WP300 C4 Inertsil Amide Inertsil HILIC InertSustain NH2 Inertsil NH2 Packing material Length 33 mm Length 50 mm 3 μm 5 μm 3 μm 5 μm Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. No. Index HPLC Catalog 1

12 Packed Guard, Packed Mini Guard Packed Guard columns Inertsil Diol Inertsil SIL-0A Inertsil SIL-150A Inertsil WP300 SIL Inertsil WP300 Diol Packing material Length 33 mm Length 50 mm 3 μm 5 μm 3 μm 5 μm HPLC Catalog

13 Packed Guard, Packed Mini Guard Packed Mini Guard columns InertSustain C18 Inertsil ODS-4 Packing material (μm) Length Cat. No Inertsil ODS Inertsil ODS Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP Inertsil ODS-80A InertSustain C8 Inertsil C Inertsil C Inertsil C Inertsil C InertSustain Phenyl Inertsil Ph Inertsil Ph Inertsil CN Inertsil WP300 C Inertsil WP300 C Inertsil WP300 C Inertsil Amide Inertsil HILIC InertSustain NH2 Inertsil NH2 Inertsil Diol Inertsil SIL-0A Inertsil SIL-150A Inertsil WP300 SIL Inertsil WP300 Diol Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. No. Index HPLC Catalog 3

14 SILFILTER TM STD C18 SILFILTER STD C18 is a guard column with cartridge size 3.0 mm mm. The cartridge is packed with a monolithic silica, chemically bonded with a C18 and uniquely end-capped based on our 45 years of experience. SILFILTER STD C18 can be installed and used for all C18 columns available in the market without interfering and infl uencing the elution or separation patterns. Also, deterioration of the packing in the cartridge can be confi rmed visually. Feature Can be installed for all C18 columns available in the market. Deterioration of the packing in the cartridge can be confi rmed visually. Easy to replace the cartridges. Effectively removes particulate and strongly retained sample compounds Delivers the lowest dead-volume. Specification Base Material : High Purity Monolithic Silica End-capping : Yes Max.Operating Pressure : 35 MPa (350 Bar) ph Range : 1~7.5 Bonded Phase : Octadecyl Groups Max.Temperature : 50 C Size : 3.0 mm Compatible analytical ID : 3.0~4.6 mm No Sacrifice in Separation Efficiency The following test was conducted to confi rm the effi ciency of SILFILTER STD C18. As shown below, the effi ciency didn t decrease even for those fast eluting samples when installing SILFILTER STD C18. C18 column only RT : 3.2 min N : 18, RT : 7.7 min N : 18, With SILFILTER STD C18 RT : 3.4 min N : 18, RT : 8.0 min N : 18, Conditions : C18 (3 μm, mm ) Eluent : A) CH3CN B) H2O A/B = 65/35, v/v Flow Rate : 0.4 ml/min Col. Temp. : 40 C Detection : UV 254 nm Sample Acetaminophen Benzene Toluene Naphthalene Time(min) Time(min) Set A (with tube) Set B (without tube) Item Cat.No. SILFILTER STD C18, Set A* 1 2 Cartridges with 1 Holder and Tubes SILFILTER STD C18, Set B * 2 2 Cartridges with 1 Holder, without Tubes SILFILTER(with Tubes) Holder for A type* SILFILTER(without Tubes) Holder for B type* Replaceable Cartridge, 2 ea PEEK Tough Connector, 5 pcs * 1: Tube size is, 1/16 inch O.D mm 30 mml * 2: Tubing tip length is 2.4 mm PEEK Tough Connector 4 HPLC Catalog

15 Filters, Impurity Remove Pre- Coupler Pre-column Coupler W (PCTFE) Pre-column Coupler SUS Precolumn Coupler is an easy-to-use tool to connect guard columns and analytical columns. Pre-column Coupler has two designs, one is using PCTFE material, except common organic solvents, acidic solvent and basic solvent also can be used. And another one is stainless design, it can be used as high pressure. Specification Max. operation pressure: 14.7 MPa (PCTFE), 80 MPa (stainless) Tube O.D.: 1/16 Item Material Connection Cat.No. Pre-column Coupler W PCTFE Waters type Pre-column Coupler SUS Stainless * * Product Pre-column Coupler SUS doe not fi xed with Ferrules, therefore columns with -32UNF specifi cation all can be used. Reversed Phase HILIC Normal Phase Pre- Filter Pre-column fi lter Connect with 1/16 fitting Filter φ 0.50 Specification Fit in tube O.D. : 1/16 Screw specifi cation : -32UNF Filter pore size : 2 μm Max. operation pressure : 41.4 MPa(SUS) 34.5 MPa(PEEK) Item Jacket material P/N Qty. (pc) Cat.No. Pre-column fi lter 2 μm Stainless A Replacement pre-column fi lter 2 μm A PEEK pre-column fi lter 2 μm PEEK A Replacement PEEK pre-column fi lter 2 μm A SEC Ion Exchange Application Specifi c Pre-Clean ORG A guard column to install between the pump and injector, to remove the impurity in aqueous eluent. Protect analytical LC columns, and prolong analytical columns lifetime. Easy-to-replace. Also can be used as an in-line fi lter, semimicro columns are most appropriate for column clog. 2 types for two different fl ow rates. Pre-clean ORG Item Length Recommend fl ow rate (ml/min) Cat.No. Pre-clean ORG replacement cartridge 2ea ~20 ml/min Pre-clean ORG holder and cartridgex2ea ~20 ml/min Pre-clean ORG holder Pre-clean ORG semi replacement cartridge 2ea ~2.0 ml/min Pre-clean ORG semi holder and cartridgex2ea ~2.0 ml/min Pre-clean ORG semi holder Guard Preparative Capillary Applications Cat. No. Index HPLC Catalog 5

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