ab FirePlex mirna Panel Neurology V2

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1 Version 1 Last updated 8 March 2017 ab FirePlex mirna Panel Neurology V2 This product is for research use only and is not intended for diagnostic use. Copyright 2017 Abcam. All rights reserved. FirePlex

2 Table of Contents 1. Overview 2 2. FirePlex mirna Panel Neurology V Notes 6 ab mirna Panel Neurology V2 1

3 1. Overview The FirePlex Neurology panel has been designed to profile expression of 65 mirnas differentially regulated in normal and abnormal neurological or psychiatric health. Our multiplex mirna assays use our FirePlex particle technology to profile up to 65 mirnas per well. With the same sensitivity as PCR, they can be used either directly with 10 µl of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA. For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit These mirnas have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in whole blood, plasma, serum, peripheral blood mononuclear cells (PBMCs) or isolated exosomes. Included in the panel are general markers for multiple neurologyrelated diseases, markers specific to specific nervous system diseases and CNS cancers, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls to validate assay performance. It is important to use the normalization procedure in the FirePlex Analysis Workbench Software to identify the mirnas that can optimally be used for normalization with your samples. Three mirnas that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel. ab mirna Panel Neurology V2 2

4 2. FirePlex mirna Panel Neurology V2 To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab for use with purified RNA or ab for use with biofluids (see these kits for the full assay protocol). FirePlex is a registered trade mark in the United States and is an unregistered trade mark elsewhere. This product is currently only supported for customers in North America, Japan and Europe, if you are outside these areas please contact us. ** Hu = Human, Ms = Mouse, Rt = Rat, Ath = Arabidopsis thaliana, Oan = Ornithorhynchus anatinus, Cel = Caenorhabditis elegans. # mirna 21 name mirna Sequence (mirbase V21) Sequence homology ** MIMAT ID 1 hsa-let-7b-5p ugagguaguagguugugugguu Hu, Ms, Rt MIMAT hsa-let-7d-5p agagguaguagguugcauaguu Hu, Ms, Rt MIMAT hsa-let-7f-5p ugagguaguagauuguauaguu Hu, Ms, Rt MIMAT hsa-let-7g-5p ugagguaguaguuuguacaguu Hu, Ms, Rt MIMAT hsa-let-7i-5p ugagguaguaguuugugcuguu Hu, Ms, Rt MIMAT ath-mir167d ugaagcugccagcaugaucugg Ath MIMAT hsa-mir-103a-3p agcagcauuguacagggcuauga Hu, Ms, Rt MIMAT hsa-mir-107 agcagcauuguacagggcuauca Hu, Ms, Rt MIMAT hsa-mir-124-3p uaaggcacgcggugaaugcc Hu, Ms, Rt MIMAT hsa-mir-125b-5p ucccugagacccuaacuuguga Hu, Ms, Rt MIMAT hsa-mir-128-3p ucacagugaaccggucucuuu Hu, Ms, Rt MIMAT hsa-mir p gaucucacuuuguugcccagg Hu MIMAT hsa-mir-132-3p uaacagucuacagccauggucg Hu, Ms, Rt MIMAT hsa-mir-134-5p ugugacugguugaccagagggg Hu, Ms, Rt MIMAT hsa-mir-142-3p uguaguguuuccuacuuuaugga Hu, Ms, Rt MIMAT hsa-mir-145-5p guccaguuuucccaggaaucccu Hu, Ms, Rt MIMAT ab mirna Panel Neurology V2 3

5 17 hsa-mir-146a-5p ugagaacugaauuccauggguu Hu, Ms, Rt MIMAT hsa-mir-150-5p ucucccaacccuuguaccagug Hu, Ms, Rt MIMAT hsa-mir-151a-3p cuagacugaagcuccuugagg Hu MIMAT hsa-mir-155-5p uuaaugcuaaucgugauaggggu Hu MIMAT hsa-mir-15a-5p uagcagcacauaaugguuugug Hu, Ms MIMAT hsa-mir-15b-3p cgaaucauuauuugcugcucua Hu, Ms, Rt MIMAT hsa-mir-15b-5p uagcagcacaucaugguuuaca Hu, Ms, Rt MIMAT hsa-mir p ccaauauuacugugcugcuuua Hu MIMAT hsa-mir-16-5p uagcagcacguaaauauuggcg Hu, Ms, Rt MIMAT hsa-mir-17-5p caaagugcuuacagugcagguag Hu, Ms, Rt MIMAT hsa-mir-181b-5p aacauucauugcugucggugggu Hu, Ms, Rt MIMAT cel-mir-70-3p uaauacgucguugguguuuccau Cel MIMAT hsa-mir-191-5p caacggaaucccaaaagcagcug Hu, Ms, Rt MIMAT hsa-mir-195-5p uagcagcacagaaauauuggc Hu, Ms, Rt MIMAT hsa-mir-197-3p uucaccaccuucuccacccagc Hu MIMAT hsa-mir-206 uggaauguaaggaagugugugg Hu, Ms, Rt MIMAT hsa-mir-20a-5p uaaagugcuuauagugcagguag Hu, Ms, Rt MIMAT hsa-mir-210-3p cugugcgugugacagcggcuga Hu, Ms, Rt MIMAT hsa-mir-214-3p acagcaggcacagacaggcagu Hu, Ms MIMAT hsa-mir-21-5p uagcuuaucagacugauguuga Hu, Ms, Rt MIMAT hsa-mir-22-3p aagcugccaguugaagaacugu Hu, Ms, Rt MIMAT hsa-mir-23a-3p aucacauugccagggauuucc Hu, Ms, Rt MIMAT hsa-mir-24-3p uggcucaguucagcaggaacag Hu, Ms, Rt MIMAT hsa-mir-26b-5p uucaaguaauucaggauaggu Hu, Ms, Rt MIMAT hsa-mir-29b-3p uagcaccauuugaaaucaguguu Hu, Ms, Rt MIMAT hsa-mir-301a-3p cagugcaauaguauugucaaagc Hu, Ms, Rt MIMAT hsa-mir-30e-5p uguaaacauccuugacuggaag Hu, Ms, Rt MIMAT hsa-mir-323a-3p cacauuacacggucgaccucu Hu, Ms, Rt MIMAT hsa-mir-328-3p cuggcccucucugcccuuccgu Hu, Ms, Rt MIMAT hsa-mir-331-5p cuagguauggucccagggaucc Hu, Ms MIMAT hsa-mir-338-3p uccagcaucagugauuuuguug Hu, Ms MIMAT hsa-mir-342-3p ucucacacagaaaucgcacccgu Hu, Ms, Rt MIMAT hsa-mir-346 ugucugcccgcaugccugccucu Hu MIMAT hsa-mir-34a-5p uggcagugucuuagcugguugu Hu, Ms, Rt MIMAT ab mirna Panel Neurology V2 4

6 51 hsa-mir-34b-3p caaucacuaacuccacugccau Hu MIMAT hsa-mir-34c-5p aggcaguguaguuagcugauugc Hu, Ms, Rt MIMAT hsa-mir-370-3p gccugcugggguggaaccuggu Hu, Ms, Rt MIMAT hsa-mir-382-5p gaaguuguucgugguggauucg Hu, Ms, Rt MIMAT hsa-mir-451a aaaccguuaccauuacugaguu Hu, Ms, Rt MIMAT hsa-mir-483-3p ucacuccucuccucccgucuu Hu MIMAT hsa-mir-486-5p uccuguacugagcugccccgag Hu, Ms, Rt MIMAT hsa-mir-491-5p aguggggaacccuuccaugagg Hu, Ms MIMAT hsa-mir-497-5p cagcagcacacugugguuugu Hu MIMAT hsa-mir p uuuugugucucccauuccccag Hu MIMAT hsa-mir-532-5p caugccuugaguguaggaccgu Hu, Ms MIMAT hsa-mir-545-3p ucagcaaacauuuauugugugc Hu, Ms, Rt MIMAT hsa-mir-7-5p uggaagacuagugauuuuguugu Hu, Ms, Rt MIMAT oan-mir p uuccccacucugagcacacagc Oan MIMAT hsa-mir-885-5p uccauuacacuacccugccucu Hu MIMAT hsa-mir-92a-1-5p agguugggaucgguugcaaugcu Hu MIMAT hsa-mir-93-5p caaagugcuguucgugcagguag Hu, Ms, Rt MIMAT hsa-mir-98-5p ugagguaguaaguuguauuguu Hu, Ms, Rt MIMAT x-control x-control N/A 70 blank blank N/A ab mirna Panel Neurology V2 5

7 3. Notes ab mirna Panel Neurology V2 6

8 Technical Support Copyright 2017 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. Austria wissenschaftlicherdienst@abcam.com France supportscientifique@abcam.com Germany wissenschaftlicherdienst@abcam.com Spain soportecientifico@abcam.com Switzerland technical@abcam.com Deutsch: Français: UK, EU and ROW technical@abcam.com +44(0) Canada ca.technical@abcam.com US and Latin America us.technical@abcam.com Asia Pacific hk.technical@abcam.com (852) China cn.technical@abcam.com Japan technical@abcam.co.jp +81-(0) Singapore sg.technical@abcam.com Australia au.technical@abcam.com +61-(0) New Zealand nz.technical@abcam.com +64-(0) Copyright 2017 Abcam. All rights reserved. FirePlex