Read the whole of this document before unpacking your Izon equipment or proceeding to the steps below.

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Getting Started Read the whole of this document before unpacking your Izon equipment or proceeding to the steps below. 1. Take the USB drive out of your training kit and put the rest of the contents in a refrigerator. 2. Read the qnano User Manual which can be found as PDF files on the USB drive. The User Manual has detailed instrument descriptions and installation information. 3. Install the Izon Control Suite Software (CSS): a. Insert the Izon USB drive into your computer. b. An auto run prompt will ask you to run Install.exe. 4. Once Izon Control Suite software and instrument are both successfully installed, complete reading the User Manual and proceed to undertake the training course described in the Izon s TRPS Training Module PDF document on the USB drive. Important: Do not attempt to operate the equipment until successfully completing the Izon Training Programme. Additional support material is available on the support portal (KBase), sign up here: Website URL: https://izon.freshdesk.com/support/login If you have any questions that are not answered on the KBase, please contact our Support staff via KBase or directly by email or skype: E-mail: support@izon.com Skype: IzonSupport QN1-IQ-013(F)

Important Read Before Operating Izon Instrumentation Follow all directions in this document and complete the Izon Training Module before attempting to operate the instrument, they contain important safety and operational information. Use of this instrument in a manner not specified by the instructions in this guide and the training module may impair the safety protection provided. Do not operate the instrument outside its rated supply voltages or environmental range Failure to read these instructions and the training module and use the instrument in the manner specified may result in impaired performance and damage to the equipment. The following symbols are used on the instrument and in this manual:- Earth (ground) terminal. Direct Current (DC) Voltage polarity of Jack. USB Connection Caution risk of danger or impaired operation, if instructions not followed Important please read below for installation instructions and an explanation on connecting and operation of the instrument.

Izon Instrument Components and Installation qnano qmicro qviro-x See the qnano User Manual.pdf located in the Training Module folder on the USB drive from the training kit. See the qmicro User Manual.pdf in the Guides folder on the USB drive from the training kit. See the qviro-x Getting Started Guide in the Guides folder on the USB drive from the training kit. Powering Your Instrument IMPORTANT Plug into Earth Grounded Protected Outlets ONLY. Make sure power supply box is positioned off benches and away from fluids. To prevent heat build-up, do not cover the power-supply box. Position unit so it can easily and quickly be disconnected from mains power. Mains Operating Voltage This instrument has a universal input range and will operate from a nominal 115 V or 230 V mains supply without adjustment. Check that the local supply meets the AC Input requirement given in the Specification. Izon Instruments are only to be operated with Izon supplied leads and power supply. Failure to use the correct power supply may result in invalid operation. Do not place the power supply in a position where it can come into contact with spills or moisture. Make sure the power supply is placed away and to the rear of the instrument to avoid spills during filling the fluid cell. Computer Equipment Set-up Devices that can be connected to the equipment should be compliant with a relevant safety standard such as IEC 60950-1 for IT equipment or IEC 61010-1 for laboratory equipment and should provide double or reinforced insulation from hazardous voltage sources.

Do not place any computer for connecting to the Izon instrument in a position where it can come into contact with spills or moisture. Make sure Izon supplied USB cable is used. General Operating Precautions For indoor use only, to be used within the rated condition in system specifications Take care not to spill fluids on electrical parts during operation Repair and Servicing of your Izon Instrument There are no user serviceable parts. Return equipment to Izon for service. All replacement parts, cables, mains-leads, power-supply, nanopores, fluid cells must be obtained via Izon Science Ltd. Cleaning of your Izon Instrument Care and cleaning of your instrument is important for correct operation. Failure to follow the proper care instructions may result in impaired operation. Please refer to the following product documents for cleaning and care instructions; qviro-x & qnano 21 CFR part 11 installs qnano qmicro Refer to the SOP for Performing Installation Qualification Protocol for a qnano manual, section 10. Follow the instructions in Section 5 of the qnano User Manual Refer to the qmicro User Manual Take care with cleaning not to spill excess fluid on other equipment Consumables Ordering For ordering of nanopores, calibration particles, and other consumables, please see our online store at store.izon.com.

System Specification Item Specification Comment Environment Indoor use at altitudes up to 2000 m, Pollution Degree 2 20% to 80% RH. Operating Temperature 5 C to 25 C RMS noise performance i Typical < 10 pa For +/- 200 na range NP200 Data sampling rate 50,000 samples per second USB standard connector Max input voltage USB 2.0 Mini-B 5.00 ± 0.25 V Cable must be to relevant safety standard such as IEC 60950-1 for IT equipment or IEC 61010-1 for laboratory equipment and should provide double or reinforced insulation from hazardous voltage sources Electrical Power ii Voltage, current Power consumption 12 V (DC ), 130 ma 1.5 W At input jack on instrument PSU Output DC Input AC Output current max Output power max 90 to 264 Vac 50/60 Hz ac 12 V nom 3.75 A 45 W iii Use only Izon supplied TRG45A120-21E11 Nanopore holding stage extension range Min 41mm Max 61mm Measured across the outside of opposing holding arms. Electrical safety IEC/EN 61010-1 EMC IEC/EN 61326-1 i Under electrical interference noise may increase but will return to normal operation on end of interference. Position equipment away from electrical switching gear and interfering equipment or noise may increase under sample running conditions. ii For no equipment plugged into the Izon auxiliary interface. iii PSU module rating. Izon Instruments running normally are approx. less than 1.6W