Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Thank yu fr purchasing an Agilent G5574A NGS Wrkstatin Optin B. This checklist is used by the installing engineer t ensure that the instrument and assciated systems are crrectly installed, upgraded and functining as designed in yur facility. This checklist will be cmpleted at the end f the service and prvided t yu as a recrd f the installatin. Custmer Infrmatin Custmers shuld prvide all necessary perating supplies upn request f the engineer. Service Engineer s Respnsibilities Only cmplete/printut pages that relate t the system being installed. Cmplete empty fields with the relevant infrmatin. Cmplete the relevant checkbxes in the checklist using a X r tick mark in the checkbx. Cmplete Nt Applicable check bxes t indicate services nt delivered, as needed. Cmplete the Service Review sectin tgether with the custmer. Additinal Instructin Ntes IMPORTANT: Save the packing materials and shipping cntainers in case yu are required t mve r ship the devices. Packing the devices using ther materials might result in damage and vid yur warranty. Ensure that yu have the fllwing guides: G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001) BenchCel Micrplate Handler User Guide (part number G5580-90000), includes unpacking and installatin instructins Brav Platfrm Unpacking Guide (part number G5523-90000) Nte: This guide is attached t the shipping cntainer. G5562A, G5563A Brav Platfrm Safety and Installatin Guide (part number G5562-90001) Brav Platfrm User Guide (part number G5562-90000) Labware MiniHub Safety and Installatin Guide (part number G5484-90004) PlateLc Thermal Micrplate Sealer User Guide (part number G5402-90001), includes unpacking and installatin instructins Nte: The Brav and MiniHub unpacking guides are attached t their shipping cntainers. The fllwing figures shw the installatin cnfiguratin withut the ptinal PlateLc Sealer. Issued: 18 August 2017, Revisin: 1.0 Cpyright 1 2017 Agilent Technlgies Page 1 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Figure. NGS Wrkstatin Optin B cnfiguratin (frnt view) Figure item Cmpnent name 1. Cmputer and mnitr 2. Brav Platfrm with gripper preinstalled 3. Liquid-handling head (96LT Head, standard) 4. Brav risers Nte: Risers are required t accmmdate the deck accessries. 5. Brav Light Curtain and shields 6. BenchCel Micrplate Handler, 4R with fur 660-mm labware racks and BenchCel shield Nte: If the BenchCel device is n risers, the Labware MiniHub must als be n risers. 7. Labware MiniHub 8. Emergency-stp pendants 9. Rbt Disable Hub 10. Ethernet switch 11. Inhec MTC Cntrller Nte: Cntrls the heating and shaking accessries installed n Brav deck. 12. Therm Cube (nt shwn - typically underneath the table) Nte: Supplies chilled fluid t the Thermal Statin (cling pad) installed n Brav deck. Issued: 18 August 2017, Revisin: 1.0 Cpyright 2 2017 Agilent Technlgies Page 2 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Figure. NGS Wrkstatin Optin B cnfiguratin (tp view) System Infrmatin Guidance Check this bx if an instrument cnfiguratin reprt is attached instead f cmpleting the table. Instrument system name and ID Instrument system site and lcatin List system cmpnent prduct numbers 1. List the serial numbers f each cmpnent 2. 3. 4. 5. 6. Issued: 18 August 2017, Revisin: 1.0 Cpyright 3 2017 Agilent Technlgies Page 3 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist 7. 8. 9. 10. 11. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. Issued: 18 August 2017, Revisin: 1.0 Cpyright 4 2017 Agilent Technlgies Page 4 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist General Preparatin Verify that the Site Preparatin Checklist has been fllwed and fully cmpleted. Measure the main utlet line vltage VAC. Unpack/verify the cnditin and cmpleteness f shipment. Fr unpacking instructins, see the crrespnding unpacking r user guide. Fr discrepancies, use the table belw. Prduct r part descriptin Observatin Actin CAUTION Ensure that the y-axis shipping stp remains installed n the Brav head munt until after yu install the Brav risers t prevent ptential equipment damage. Discuss any specific questins r issues with the custmer prir t starting. Discuss any cnfiguratin ptins with the custmer prir t starting. Determine whether the BenchCel and MiniHub devices will be installed n risers. Check fr required service nte applicability and firmware updates if cnnecting t instruments. Check that custmer-supplied PC hardware meets minimum requirements e.g. Site Preparatin Tl http://www.chem.agilent.cm/en-us/technical-supprt/sftware- Infrmatics/Utilities/Pages/SWSitePrepTl.aspx Upgrades nly Ensure with custmer that instrument cntrl settings, data, methds, and s frth have been prperly saved r archived prir t starting any installatin prcedures. Task 1 - Install and set up the cmputer See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). Cnnect the cmputer, mnitr, muse, and keybard. Verify that the cmputer is running Micrsft Windws 10 64-bit perating system and has VWrks sftware versin 13.1 installed. Verify the NGS Optin A flder structure: The FSE Test Optin B flder, typically installed at C:\VWrks Wrkspace\NGS Optin B\FSE Testing Optin B Issued: 18 August 2017, Revisin: 1.0 Cpyright 5 2017 Agilent Technlgies Page 5 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Task 2 - Install the Brav Platfrm See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). Install the Brav 146-mm risers. Remve the y-axis shipping stp frm the head munt. Cnnect the Brav pwer crd. Cnnect the Brav cmmunicatins cable using an Ethernet switch. Install the Rbt Disable Hub. Cnnect the 2 pendants and 4 e-stp jumpers. Cnnect the device prt n the hub t the Brav pendant prt. Task 3 - Install the Brav accessries and pipette head See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). Figure. Brav deck cnfiguratin (tp view) Install the Thermal Statin (cling pad) at deck lcatin 9. Cnnect and fill the Therm Cube, and turn n the pwer. Install the tw Peltier Thermal Statins (CPAC2) at deck lcatins 4 and 6. D nt install the plate inserts at this stage. Install the PCA bards in the Inhec MTC Cntrller fr the Peltier Thermal Statins. Cnnect and turn n the pwer. Install the Orbital Shaking Statin at deck lcatin 5. Cnnect and turn n the pwer. Prepare the Magnetic Bead Accessry fr installatin at deck lcatin 7. D nt install the accessry at this stage. Ensure pipette head is ready t install (tip bx stripper pins are retracted), and install the head. Fr instructins, see the Brav Platfrm User Guide (part number G5562-90000). Issued: 18 August 2017, Revisin: 1.0 Cpyright 6 2017 Agilent Technlgies Page 6 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Task 4 - Install the BenchCel Micrplate Handler See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). If applicable, install the BenchCel risers. Munt the rbt head n the BenchCel x-axis carriage. Integrate the BenchCel Riser r integratin plate with the Brav Riser. Cnnect the BenchCel pwer and the air surce. Cnnect the BenchCel pendant and pendant prt thrugh the Rbt Disable Hub. Cnnect the BenchCel cmmunicatins cable t the Ethernet switch. Task 5 - Install the Labware MiniHub See G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). If applicable, install the MiniHub base n the MiniHub iplate Riser assembly. Integrate the MiniHub Riser assembly with the BenchCel riser, if applicable. Otherwise, integrate MiniHub iplate with BenchCel iplate. Inspect the 5 shelves t ensure that they are flat. Assemble the MiniHub shelves and spacers per the specified cnfiguratin. Cnnect the AC pwer supply. Cnnect the cmmunicatins cable. Ensure that the prper drivers are installed fr the DB9-t-RJ45 adapter and USB-t-serial adapter. Task 6 - Turn n the devices and cmplete the installatin See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). Ensure that pwer is n fr the Brav accessries. At Rbt Disable Hub, ensure the LIGHT CURTAIN switch remains set t BYPASS. Ensure that the cmputer is n. Turn n the BenchCel air supply and pwer. Wait fr the rbt t finish hming. Install the labware racks n the BenchCel device. Turn n the Brav Platfrm. Task 7 - Verify VWrks device files and Brav prfiles See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). In the VWrks sftware, check the device file: BravMiniPHBenchCel_rund_magnet.dev. In Brav Diagnstics, verify the Brav-Mag and Shaker prfile settings and initialize the prfile. Verify the deck cnfiguratin in the prfile and test the accessries. Set and verify the pipette head teachpints fr the prfile: Use a single pipette tip t set the teachpint at Brav deck lcatin 2. Issued: 18 August 2017, Revisin: 1.0 Cpyright 7 2017 Agilent Technlgies Page 7 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Remve the single tip and munt a full clumn f tips. Using a feeler gauge, set the teachpints at Brav deck lcatins 1, 3, 8; at lcatins 4 and 6; at lcatin 5 using a teaching plate; and at lcatin 7. Verify the teachpint z-axis values. Munt a full head f pipette tips. Verify the Brav deck lcatin 7 teachpint. Remve the pipette tips frm head. Save the prfile and remve the pipette tips. Verify the gripper y-axis ffset and test the pick-and-place peratins. Clse Brav Diagnstics, and clse the device file. Create a secnd device file, BravMiniPHBenchCel_rund_magnet_wash.dev, and create a new Brav-2 Inserts Mag and Shaker prfile: Adjust the z-axis teachpint at deck lcatin 4 fr the Red PCR Plate Insert. Adjust the z-axis teachpint at deck lcatin 6 fr the Deepwell Plate Insert. In the device file, relink the Brav-2 Inserts Mag and Shaker prfile and save the changes. Task 8 - Set up the BenchCel and MiniHub See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). In BenchCel Diagnstics, initialize the BenchCel device. Verify that the BenchCel dwnstacks and upstacks tip bxes crrectly and that the sensrs are detecting the tip bxes. Set and verify the BenchCel teachpint at Brav deck lcatin 3 and tighten the screws n the Brav- BenchCel integratin clamp. In MiniHub Diagnstics, initialize the MiniHub device. Set the MiniHub hme psitin at cassette 1 and tighten the BenchCel-MiniHub clamp screw. Set and verify the BenchCel teachpints fr MiniHub cassette 1, shelves 1 t 5. Task 9 - Install and test the safety equipment See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). Install the BenchCel shields. Install the Brav Light Curtain and cnnect the transmitter and receiver cables t the Rbt Disable Hub. At the Rbt Disable Hub, set the LIGHT CURTAIN switch t ENABLE. Install the Brav frnt tp and bttm shields. Install the Brav side shields and rear shield, if applicable. Issued: 18 August 2017, Revisin: 1.0 Cpyright 8 2017 Agilent Technlgies Page 8 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Task 10 - Install the PlateLc Sealer See the PlateLc Thermal Micrplate Sealer User Guide (part number G5402-90001). Sectin NOT Applicable Unpack the PlateLc Sealer. Cnnect the PlateLc Sealer t the pwer surce. Cnnect the PlateLc Sealer t the air surce. Remve the small blck f fam packed inside the PlateLc Sealer. Task 11 - Validate the wrkstatin setup See the G5574A NGS Wrkstatin Optin B Installatin Guide (part number G5574-90001). Test the safety equipment t ensure that interrupting the Light Curtain r pressing either pendant will cause an emergency stp f bth the Brav and BenchCel devices. Perfrm a simulated run f the test prtcl. Perfrm a mck run f the test prtcl. Service Review Attach available reprts/printuts t this dcumentatin. Recrd the time/date f installatin r upgrade cmpletin in the custmer s recrds/lgbk. Cmplete the Service Engineer Cmments sectin belw if there are additinal cmments. Review the installatin/upgrade with the custmer. Explain Agilent warranty fr instruments. Explain hw t use manuals, guides, and nline help. Explain hw t get self-help, FAQs frm the web. Explain hw t lg an instrument service call, supprt services available. Advise custmer f additinal instrument training ptins. If the Instrument firmware was updated, recrd the details f the change in the Service Engineer s Cmments bx belw r if necessary, in the custmer s IQ recrds. Service Engineer Cmments (ptinal) If there are any specific pints yu wish t nte as part f perfrming the installatin r ther items f interest fr the custmer, please write in this bx. Issued: 18 August 2017, Revisin: 1.0 Cpyright 9 2017 Agilent Technlgies Page 9 f 10
Agilent G5574A NGS Wrkstatin Optin B Installatin Checklist Other Imprtant Custmer Web Links Fr additinal infrmatin abut Agilent Autmatin Slutins, please visit http://www.agilent.cm/en-us/prducts/autmatin-slutins Need t get infrmatin n yur prduct? NGS Sample Preparatin Autmatin prduct page - http://www.agilent.cm/en/prducts/autmatin-slutins/ngs-sample-preparatinautmatin/ngs-wrkstatin Need Supprt Services? Service Cmpletin VWrks Knwledge Base - http://www.agilent.cm/chem/askb Agilent Library - http://www.agilent.cm/en-us/library/literature www.agilent.cm/crsslab Field Engineer GSCA Supprt Center - https://fsg-assist.chem.agilent.cm Service request number Date service cmpleted Agilent signature Custmer signature Number f pages in this dcument Dcument part number: G5574-90003 Issued: 18 August 2017, Revisin: 1.0 Cpyright 10 2017 Agilent Technlgies Page 10 f 10