China Journal of Chinese Materia Medica

Transcription

1 China Journal of Chinese Materia Medica Vol. 29, Issue Northeast Agicultural University, Harbin , China ; 3. Heilongjiang Academy of Traditional Chinese Medicine, Harbin , China ) [ Abstract] Objective : To study the correlation between morphological variation and gentiopicroside content in cultivated Gentiana manshurica roots and to investigate the feasibility of breeding new varieties of high effective constituent content. Method : Gentiopicroside was determined in 5 morphological types of cultivated G. manshurica roots by HPLC, which are normal (or wild) type, white2flowered type, thick2rooted type, broad2leaved type I and broad2leaved type. Result : Among different types gentiopicroside content is the highest in the roots of thick2rooted type, the contents decrease as following order : normal type, broad2leaved type I white2flowered type and broad2leaved type II, and the gentiopicroside contents in the same type root system are a positive correlation with root ages, as 32years2age roots > 22years2 age roots > 12year2age varied with roots. Conclusion : The contents of effective constituent vary with the morphological variation in cultivated G. manshurica. It is feasible to breed a new variety with high effective constituent with the morphological character as a selecting index. [ Key words] Gentiana manshurica ; gentiopicroside ; HPLC [ ] 1 3, 1, 1, 1, 1, 1, 2, 1 1, (1., ; 2., ) [ ] : : SPSS : ;,, : [ ] ; ; [ ] S 567 [ ] A [ ] (2004) Salvia 1 miltiorrhiza Bge (, ), (1), 20 70,,,,, 1, [ ] [ ] ( ) [ ] 3,Tel : (010) , Fax : (010) E2mail com 844 / ( ) ( ) ( ) ( ) ( )

2 China Journal of Chinese Materia Medica Vol. 29, Issue [1 ] 1. 2 ph :, 1 1,ZD - 2 ) : : CuSO 4 2K 2 SO 4, ( ) ( ), : 0. 5 mol L - 1 NaHCO 3 2,, 721 ( :1 mol L - 1 NH 4 Ac,6400 ) ( ) ( ), : HClO 4 2HF,ICP 1. 3 : 230 %,, : 5 %HNO 3,,ICP 1. 4 SPSS 10. 0,,,, [2 ] ) (,, [3 ],, ; (, [4 2 ] ), 2. 1 ( ), ; 2. 2 ; ; ; ; ;, [4 : ] (2),, S. przewalskii, ( ), ( ), ( ),, 2 ( (2) 2 / % ( ) ( ) ( ) ( )

3 China Journal of Chinese Materia Medica Vol. 29, Issue ,, :,, ;, ;, ;, ;,,, 1) ph N 1) 3 P K Ca Mg Na Fe Mn Zn Cu B : N %, mg kg - 1 ( ) (0. 717) ( ) (46), (0. 580) (0. 523) ; 3 :, ( ) (0. 558) (0. 526) ; 4 4, : ( ), ( ) 12,,, ; ; ; ; ( > 0. 5 ), 11, ( ) 5, 4, %, 6, (, 1 : (0. 764) ), (0. 674) (0. 650) (0. 582) (0. 582) 2. 3,, ; ( ) ; 2 : ; 846

4 China Journal of Chinese Materia Medica Vol. 29, Issue N P K Ca Mg Na Fe Mn Zn Cu B P Sig. (22tailed) N P ) Sig. (22tailed) P P Sig. (22tailed) K P ) ) Sig. (22tailed) Ca P , Sig. (22tailed) ,698 Mg P ) Sig. (22tailed) Na P ) ) ) Sig. (22tailed) Fe P ) Sig. (22tailed) Mn P ) Sig. (22tailed) Zn P ) Sig. (22tailed) Cu P ) ) Sig. (22tailed) B P ) ) Sig. (22tailed) : P Pearson( ), Sig. (22tailed), 1) P < ; 2) P < (8 ) 5 ; ; / % / % N P,,, 6 K Ca Mg Na Fe Mn Zn Cu B ) , ; ; ( P < 0. 01),, ( ; ;, ( P < ) 0. 05), 3, 11, ( ) %(10), ( ), 1 : ( ) ( ), (0. 750) ( ) ( ) ( ),, (0. 598) (0. 597) ; 2 3 ; :(0. 734) ( ) (0. 562) ; 3 : (0. 708) (0. 532) ( 847

5 China Journal of Chinese Materia Medica Vol. 29, Issue N P K Ca Mg Na Fe Mn Zn Cu B : N,P,K % ;Ca, Mg, Na mg kg N P K Ca Mg Na Fe Mn Zn Cu B N P Sig. (22tailed) P P Sig. (22tailed) K P ) Sig. (22tailed) Ca P ) Sig. (22tailed) Mg P ) Sig. (22tailed) Na P ) ) Sig. (22tailed) Fe P ) ) Sig. (22tailed) Mn P ) Sig. (22tailed) Zn P ) ) Sig. (22tailed) Cu P ) Sig. (22tailed) B P ) ) Sig. (22tailed)

6 China Journal of Chinese Materia Medica Vol. 29, Issue / % / % ,, 10 N P K Ca Mg Na Fe Mn Zn Cu B P K Ca Mg Na Fe Mn Zn Cu B ,,, [5 ],,,,,,,, ph ( ),,,,,,,,,,, ( ,, 1,2 ), (, ),, ( ), 1 %, %, ( ), 15, (5 %10 %) ( < 5 %) [ ], [1 ].. :,1978. [2 ]. (SPSS for windows). : 3. 2, ,

7 China Journal of Chinese Materia Medica Vol. 29, Issue [3 ],,,. [5 ],... :,1990..,1998, 29( ) :121. [4 ],,,.. [6 ]..,,12 (6) : (10) :56. Content of inorganic elements of Salvia miltiorrhiza root in different area and physicochemical properties of its growing soil ZHAO Yang2jing 1, CHEN Si2bao 1, GAO Gua2yao 1, FENG Yu2xiu 1, YANG Shi2lin 1, XU Li2zhen 1, DU Li2jun 2, LIN Jia 1, LI Min 1 (1. Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing , China ; 2. Tsinghua University, Beijing , China) [ Abstract] Objective : To provide basis of environmental factors of genuine crude drug, Salvia miltiorrhiza root. Method : On2the2spot investigation and indoor chemical analysis were made to study the physicochemical properties of growing soil and content of inorganic elements of S. miltiorrhiza, and obtained data were analysed with SPSS software. Result : S. miltiorrhiza root of high harvest area accumulated Cu and Zn. In soil principal component analysis, no principal component was obvious. So the drug has good adaptability in ecological environ2 ment of soil. Conclusion : Ecological environment of soil isn t leading factor in forming genuine crude S. [ Key words] Salvia miltiorrhiza ; content of inorganic elements ; soil miltiorrhiza. [ ] 1 1, 3, 1 2, (1., ; 2., ) [ ] : (FTIR) (HPLC), :,,, A(crocin A) 23 :,, [ ] ; ; HPLC ; FTIR [ ] S 567 [ ] A [ ] (2004) Crocus sativus L., [1 ],, [225, ], (crocin A),, [ ] [ ] 3, Tel : (010) , E2mail : inghua. edu. cn 850