Bio-Pyramid / STYLUS 1s MBP01-S1SB User s Manual

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Bruce Merritt
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Bio-Pyramid / STYLUS 1s MBP01-S1SB User s Manual Standard Accessories 1) Olympus STYLUS 1s 2) Lithium ion battery (BLS-50) 3)

Instruction Manual 9) Warranty Camera Installation Camera Please install with following order. 1.

Attach Φ52mm sleeve to RA5255B 4. Attach all of above to hood body Filter Glass filter install onto φ52mm sleeve.

1 Bio-Pyramid / STYLUS 1s MBP01-S1SB User s Manual Standard Accessories 1) Olympus STYLUS 1s 2) Lithium ion battery (BLS-50) 3) Lithium ion charger (BCS-5) 4) Lithium ion charger cable 5) USB Cable (CB-USB6) 6) Lens Cap 7) PC Software (CD-ROM) 8) Instruction Manual 9) Warranty Camera Installation Camera Please install with following order. 1. Remove lens cap from the camera body 1. Attach CLA 13 to Stylus 1s lens part 2. Attach RA5255B to CLA Attach Φ52mm sleeve to RA5255B 4. Attach all of above to hood body Filter Glass filter install onto φ52mm sleeve. Gelatin filters slide it into the filter tray. ** 75mm square size filter can be used. 3 Filters ALSC56 for SYBER Green SYPRO Orange SC58:SC60 for EtBr Please start with fully charged battery

Instruction mark is important ** Please also refer to STYLUS 1S Camera manual for setting up followings.

Therefore, please set the camera to M (manual) mode. Followings are setting instruction for M (manual) mode. 1.

button and display live control. Select the image stabilizer item using. Select an ISON with and press OK button.

2 Instruction mark is important ** Please also refer to STYLUS 1S Camera manual for setting up followings. Gel documentation takes place in a darkroom environment and so is difficult to capture quality images with full automatic mode. Therefore, please set the camera to M (manual) mode. Followings are setting instruction for M (manual) mode. 1. Camera Setting (1) (This color indicate corresponding page in camera manual) 1 Select M (manual) mode by using Mode Dial ** Manual P16 Mode Dial 2 Image Stabilizer Press OK button and display live control. Select the image stabilizer item using. Select an ISON with and press OK button. ** Manual P33 OK Button 3 Monotone (black and white) Press OK button and display live control. Select the picture mode item using. Select an Monotone with and press OK button. ** Manual P33 4 ISO Sensitivity Press OK button and display live control. Select the ISO sensitivity item using. Select an 1600 with and press OK button. ** Manual P40 5 Flash Off Keep strobe inside the camera and flash won t go off Strobe

2. Camera Setting (2) adjusting aperture and shutter speed 1 Setting aperture Press on sub dial and display mark on screen. Select an F8.0 F value with sub dial.

The shutter speed can be set to minimum 1/2000 sec with maximum 16 minutes [BULB]. ** Manual P20 Fast clockwise Fn2 lever Slow counter CW Control Ring 3.

3 2. Camera Setting (2) adjusting aperture and shutter speed 1 Setting aperture Press on sub dial and display mark on screen. Select an F8.0 F value with sub dial. ** Manual P20 Fn2 lever 1) Press 2) Adjust with 2 Setting shutter speed Press on sub dial and display mark. Select an shutter speed 2 (1/2) sec with sub dial. The shutter speed can be set to minimum 1/2000 sec with maximum 16 minutes [BULB]. ** Manual P20 Fast clockwise Fn2 lever Slow counter CW Control Ring 3. Camera Setting (3) ** Manual P46 1 Long Exposure Noise Reduction Press MENU button and select a Custom Menu with sub dial and press OK button. Select an Exp/Metering/ISO with sub dial and press OK button. Select a Noise Reduct with sub dial, and set it ON and press OK button. ** Manual P69, 73 2 High ISO Noise Reduction Press MENU button and select a Custom Menu with sub dial and press OK button. Select an Exp/Metering/ISO with sub dial and press OK button. Select a Noise Filter with sub dial, and set it Standard and press OK button. ** Manual P69, 73 3 Sleep Setting Press MENU button and select a Custom Menu with sub dial and press OK button. Select an Display with sub dial and press OK button. Select a Sleep with sub dial, and set it OFF and press OK button. ** Manual P69, 72

4 Custom mode Settings (Restore above preselected settings) Press MENU button and select a Shooting Menu 1 with sub dial and press OK button.

Settings are registered to Mode dial C 1 ** Manual P26 Once registered, just set mode dial to C1 from second time Please fully charge the battery before start shooting. 4.

place Hood in a way that it covers entire emission surface. 3 Focusing sheet will become luminous once Transilluminator is turned on. **image is MBP UVCR 5.

4 4 Custom mode Settings (Restore above preselected settings) Press MENU button and select a Shooting Menu 1 with sub dial and press OK button. Select an Reset/C Mode Setup with sub dial and press OK button. From the menu, select a Custom Mode 1 with sub dial, and press OK button. Settings are registered to Mode dial C 1 ** Manual P26 Once registered, just set mode dial to C1 from second time Please fully charge the battery before start shooting. 4. Setting Up Transilluminator 1 Place focusing sheet on the Transilluminator. 2 Place Hood on the 1 Place Hood on the Transilluminator after installing camera. place Hood in a way that it covers entire emission surface. 3 Focusing sheet will become luminous once Transilluminator is turned on. **image is MBP UVCR 5. Camera Focusing and Zooming Setting 1 Focusing Focus Lock setting. Press OK button and set focusing with sub dial. Select S AF with sub dial, and press OK button. ** Manual P.39 Select focus target Press sub dial and display the AF target. With sub dial, select the AF target to which you want to focus on and press OK button. ** Manual P30 OK Button

Keeping the shutter button pressed halfway, and make sure AF target position.

5mm) is Max zooming range which can be in focused.

: Instead of using focusing sheet, mini gel ruler can also be used.

electrophoresis unit and let it fluorescence. Focus Lock setting.

5 Keeping the shutter button pressed halfway, and make sure AF target position. Adjust zoom with Zoom Lever as it requires. Note: 10.7x (shooting range 43.5 x 30.5mm) is Max zooming range which can be in focused. : It is convenient to register the AF target setting to C2 once it is fixed. : Instead of using focusing sheet, mini gel ruler can also be used. Zoom Lever 2 Focusing with mini Gel Ruler Position mini gel ruler to an area which does not affect electrophoresis unit and let it fluorescence. Focus Lock setting. Press OK button and set focusing with sub dial. Select S AF with sub dial, and press OK button. ** Manual P.39 Keeping the shutter button pressed halfway, and make t AF target targeted to mini gel ruler. OK Button Adjust zoom with Zoom Lever as it requires. Zoom Lever

6. Gel Documentation Turn on the Camera Place the gel right on the center and turn on the Transilluminator.

Adjusting Brightness Adjusting brightness with Shutter Speed Shutter speed means exposure time.

(Tip: start with rough estimates and narrow a target down for precise adjustment) ** Manual P20 Longer Brighter Shorter Darker 8.

6 6. Gel Documentation Turn on the Camera Place the gel right on the center and turn on the Transilluminator. Turning the zoom lever to the right and adjust to appropriate shooting range. ** Manual P10 Zoom Lever 7. Adjusting Brightness Adjusting brightness with Shutter Speed Shutter speed means exposure time. Slow shutter speeds let more amounts of light reaches the image sensor and thus are used to brighten images. Please adjust accordingly. (Tip: start with rough estimates and narrow a target down for precise adjustment) ** Manual P20 Longer Brighter Shorter Darker 8. Manual Focus When it is difficult to focus with AF (e.g. gel is too thick, or unable to use focusing sheet), use manual focus instead. 1 Pull up Fn2 lever which is equipped on the front side of camera. 2 Rotate the Control Ring and adjust focusing position. ** Manual P31 Fn2 lever Fast clockwise Slow counter CW Control Ring LCD view will be switched to focusing area when focusing

9. Transfer Picture data to PC. You can transfer images to a computer by connecting the camera to the computer with provided USB cable and software (CD ROM)., ** Manual P86 10.

7 9. Transfer Picture data to PC. You can transfer images to a computer by connecting the camera to the computer with provided USB cable and software (CD ROM)., ** Manual P Zooming Magnification and Shooting Range Up to 10.7x (shooting range 43.5 x 30.5mm) is Max zooming range which can be in focused. For more Zooming power, please use close up lens CU 3 (optional item) 11. Saving Image / Viewing / Erasing / Printing 1 Captured images will be save in SD card ** Manual P100 2 To see captured images, please select picture from[playback mode]** Manual P11 3 To find images from the list (Index display) ** Manual P11 4 To enlarge images (Close up playback) ** Manual P12 5 Erasing Images ** Manual P12 6 Printing Images ** Manual P82 Other settings Please refer Stylus1s Instruction manual for detail. The guide is one of the standard settings for documentation and for reference purpose only. Please adjust details in accordance with your particular observing environment. White Balance When you shoot with color, please adjust white balance in accordance with color tone of light source. You will be able to capture image without any color bias. ** Manual P34 Image Size Setting image (pixel) size. Smaller data (pixel) size is easy to handle. Large data (pixel) size is preferable for printing and enlarging. ** Manual P37 Date Able to verify captured date. ** Manual P8, 92 Large size observing window (with UV shield filter) UV transmittance is less than 0.1%. It eliminates most UV rays. Even with the shield, there is still a small amount of transmittance. Therefore, please avoid observing continuously for long time.

Photo Printer *sold separately Epson Picture Mate PM225 etc. Able to print images in just 3 easy steps. **manual P82 *operation may vary depending on the model.

SC 58 cuts below 580nm and SC 60 cuts below 600nm and these can be used for EtBr. ALSC 56 cuts below 560nm and can be used for SYBR Green/SYPRO Orange.

Bio Pyramid : Specification Maximum shooting area Shooting range Include φ52mm sleeve With super mount Filter Glass filter Gelatin filter Viewer window UV shield filter Transmittance range (220

8 Photo Printer *sold separately Epson Picture Mate PM225 etc. Able to print images in just 3 easy steps. **manual P82 *operation may vary depending on the model. Please refer to manual of your printer model ** Image on the right is BP UVOR. Filters: The 3 sharp cut filters cut excitation light and transmit only emission light. SC 58 cuts below 580nm and SC 60 cuts below 600nm and these can be used for EtBr. ALSC 56 cuts below 560nm and can be used for SYBR Green/SYPRO Orange. Please use appropriate filter for your specific regent. In addition, any other filters with size 75mm square can be set onto filter tray. Bio Pyramid : Specification Maximum shooting area Shooting range Include φ52mm sleeve With super mount Filter Glass filter Gelatin filter Viewer window UV shield filter Transmittance range ( nm) Size Weight Body/Super mount mm(= interior dimension) * May vary, depends on the camera. 310mm mm NOTICE:Please select the camera that can be focused to shooting area within these ranges. Use φ52mm Use 3 inch square filter MAX 0.09% Average 0.02% (measured, not guaranteed number) W287 D227mm(=Hood External Dimensions) H 310mm (include camera mounting part) 1040g / 200g

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