Microscope ECLIPSE FN1

Transcription

1 M368E NF.1 Microscope ECLIPSE FN1 Instructions

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3 Introduction Thank you for purchasing the Nikon product. This instruction manual is written for the users of the Nikon Microscope ECLIPSE FN1. To ensure correct usage, please read this manual carefully before operating the product. It is prohibited to reproduce or transmit this manual in part or whole without Nikon's expressed permission. The contents of this manual are subject to change without notice. Although every effort has been made to ensure the accuracy of this manual, if you note any points that are unclear or incorrect, contact your nearest Nikon representative. Some of the products described in this manual may not be included in the set you have purchased. Also be sure to read the manuals for any other products that you are using with this system (the super high-pressure mercury lamp power supply, high-intensity light source, and such). WARNING and CAUTION Symbols Used in This Manual Although Nikon products are designed to provide the utmost safety during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. Damage or injury that may occur due to mishandling is unwarranted. To ensure correct usage, read the instruction manual carefully and thoroughly before using the product. Do not discard the manual; keep it handy for easy reference. Safety instructions within this manual are accompanied by the following symbols to highlight their importance. For your safety, always follow the instructions accompanying these symbols. Symbol WARNING Meaning Disregarding instructions accompanying this symbol may lead to serious injury or death. CAUTION Disregarding instructions accompanying this symbol may lead to injury or property damage. 1

4 Introduction Meaning of the symbol used on the product The symbol appearing on the product indicates the need for caution at all times during use. Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the symbol has been affixed. Caution for heat This symbol on the lamphouse of the Eclipse FN1 calls your attention on the following: The lamp and its surroundings (including the lamp house) become very hot during and immediately after lighting. Do not touch the lamp and its surroundings during and immediately after lighting to prevent the risk of burns. Make sure that the lamp and its surroundings are sufficiently cool before the lamp replacement. 2

5 Safety Precautions Take note of the following points to use this product and its system safely and correctly. WARNING 1 Intended product use This microscope system is intended primarily for microscopy and photomicrography of specimens such as sliced living cells using the dia illumination or the epi illumination. 2 Do not disassemble Disassembling the microscope or the microscope system may result in electric shock or malfunctions. Malfunctions and damage due to disassembling are unwarranted. Never attempt to disassemble any part other than the parts described in this manual. If you experience problems with the microscope or the microscope system, contact your nearest Nikon representative. 3 Read the instructions carefully To ensure safety, carefully read this manual and the manuals provided with any other equipment used with this microscope. In particular, observe all warnings and cautions given at the beginning of each manual. To use this microscope system with an epi-fl attachment: The mercury lamp (or xenon lamp) used for the epi-fl microscopy requires special handling. Make sure to see the manual provided for either the super high-pressure mercury lamp power supply or the high-intensity light source. 4 Cautions about the power cord Make sure to use the specified power cord. Using a wrong power cord may result in malfunctions or fire. The power supply for this system is classified as subject to Class I protection against electrical shock. Make sure the power supply unit is connected to an appropriate ground terminal (protective earth terminal). Refer to Chapter 7, "Specifications," for the specified power cord. To prevent electrical shock, always turn off the power switch (press it to the "O" position) on the power supply unit before attaching or detaching the power cord. 5 Cautions about heat from the light source The lamp and its surroundings (including the lamp house) become very hot during and immediately after lighting. Do not touch the lamp and its surroundings during and immediately after lighting. They become very hot and may cause burn injuries. Make sure that the lamp and its surroundings are sufficiently cool before the lamp replacement. Do not allow cloth, paper, or highly flammable volatile materials, such as gasoline, benzine, paint thinner or alcohol, to come near the lamp house while the lamp is lit or within 30 minutes after switching off the power. They become very hot and may cause fire or burn injuries. 3

6 Safety Precautions WARNING 6 Mercury lamp or xenon lamp (for an epi-fl attachment) The mercury lamp (or xenon lamp) used for the epi-fl attachment requires special handling. To use the system safely and correctly, read the warnings below and be aware of the dangers. Also carefully read the manual for the super high-pressure mercury lamp power supply (or high-intensity light source) and the manual (if provided) by the manufacturer of the lamp and follow their instructions. Warnings about the mercury lamp and the xenon lamp 1) The mercury lamp (or the xenon lamp) radiates ultraviolet light that is harmful to the eyes and skin when the lamp is turned on. Don't look at the lamp directly. Direct viewing of light from these lamps may result in blindness. 2) Gas is sealed under very high pressure inside the lamp. The pressure increases when the lamp is on. If the lamp is scratched, dirty, subjected to high external pressure or physical impact, or used beyond its operational life, the sealed gas may escape or the lamp may burst. In such cases, there is a possibility of inhaling the gas, injury by broken piece of the glass, or other accidents. 3) When the lamp is on, the lamp and its surroundings become extremely hot. Do not tough the lamp and its surroundings to prevent a burn injury and do not place flammable materials near the lamp to prevent a fire. 4) Use only the lamps specified in this manual. Using other than the specified type of lamp could result in an accident, such as a burst. Because safety is a top priority in the design of Nikon products, the hazards described above should not pose any danger as long as you heed all of the warnings and cautions in the manuals and use the system only for its intended purpose. However, the hazards described above could lead to an accident if you fail to heed all of the warnings and cautions in the manuals, if you strike the system, or if you attempt to disassemble the system. Therefore, always be sure to heed all of the warnings and cautions. 7 Turn off the lamp before changing the filter cubes (for an epi-fl attachment) Before changing the filter cubes, be sure to turn off the light source of the epi-fl attachment. If the lamp is lit and the filter cubes are changed, dangerous ultraviolet rays may be radiated. 8 Place the D/UV slider into the optical path (for the D-FL epi-fl attachment) To perform the bright-field microscopy under the episcopic illumination, the dark-field microscopy under the episcopic illumination, or the DIC microscopy under the episcopic illumination with the mercury lamp house, place the D/UV slider of the D-ES EPI ND slider into the optical path. If the D/UV slider is removed from the optical path, dangerous ultraviolet rays will be radiated and the eyes and skin may be injured. 9 Installation of the microscope To prevent the microscope from toppling over, fix the microscope FN1 body securely on a baseplate or a vibration isolator with bolts. 4

7 Safety Precautions CAUTION 1 Isolate this microscope and its system equipment from the power source during assembly, connecting/disconnecting cords, lamp replacement, and maintenance To prevent electric shock and/or malfunctions, always turn off the power switches for the system (press them to the "O" positions) and unplug the power cords from the wall outlet before assembly, connecting or disconnecting cords, lamp replacement, and cleaning the microscopy and the objectives. 2 Cautions when replacing lamps To prevent burn injuries, wait at least 30 minutes after the lamp is turned off to give it sufficient time to cool down when replacing lamps. And, to avoid electric shock or malfunctions, never attempt to replace lamps without turning off the power switches for the system (press them to the "O" positions). And then, unplug the power cords from the wall outlet. Make sure the lamp house cover is securely fitted to the lamp house after replacing lamps. Never turn on the lamp while the lamp house cover is open. When you dispose of the replaced lamp, do not break it up. Instead, dispose of the used lamp as special industrial waste or dispose of it according to the local regulations and rules. 3 Use the specified light source Use the specified light source. Using a wrong light source may cause a malfunction or a fire. For the illumination with the optical fiber Specified light source: Sumita Optical Glass Inc. Halogen light source LS-DWL-N For the illumination with the lamp house Specified lamp house: Nikon FN-LH Specified lamp: 12V 100W Long life halogen lamp (PHILIPS 7724i or ODRAM HLX 64623) Specified power supply: Nikon TE2-PS100W * In addition, when the lamp house is used for the illumination, a lamp house adapter must be attached to connect the lamp house with the microscope FN1. 4 Do not spill water or a chemical agent onto the microscope system Never allow water or a chemical agent to come into contact with the microscope system. And do not use the microscope system in circumstances where the microscope system is splashed with water or a chemical agent. Water or a chemical agent splashed onto the power supply or the power supply device for the optical fiber light source may cause a short circuit, resulting in malfunction or abnormal overheat. If the product is subject to contact with water or a chemical agent, turn off the power switches for the product (press them to the "O" positions). Then unplug the power cords from the wall outlet. Then, wipe off the water with a piece of dry cloth. If water or a chemical agent enters the product, do not use the product and contact your nearest Nikon representative. 5 Do not place any object on the top of the microscope or its system Do not place any object on the top of the microscope or its sytem. Do not cover them with a piece of cloth or so on. The system temperature will rise, resulting in malfunctions. 5

8 Safety Precautions CAUTION 6 Cautions in assembling, installing, and carrying the microscope system Take care to avoid pinching your finger or hand during the microscope system assembly and installation. Scratches or fouling such as fingerprints on optical components (lenses, filters, and so on) will degrade microscope images. Be careful to avoid scratches or direct contact with the lenses and filters when assembling the microscope system. To carry the microscope system, first detach all parts. And, tilt the microscope body while supporting it, then securely hold it by the base. Never grasp the focus knobs, eyepiece tube, sliding nosepiece, nosepiece up/down part, stage, substage, or condenser when carrying the microscope system. This may cause the part to come off, resulting in damage to the microscope system. Do not install the product in a locker or a cabinet. 7 Do not turn on the power supply and the power supply for the optical fiber light source when a cover is placed over the product. Do not turn on the power supply and the power supply for the optical fiber light source when a cover is placed over the product. A cover on the product blocks the heat radiation and it may cause abnormal heat or fire hazards. 8 Cautions about long and continuous observations To relieve fatigue resulting from long observation sessions, limit continuous observations to one hour. Take at least 10- to 15-minute breaks between observation sessions. And, take care of the layout of devices to be used and adjust the height of your chair. 6

9 Notes on Handling the Microscope System Notes on Handling the Microscope System 1 Handle with care This microscope system is a precision optical instrument. Handle the microscope system with care to avoid shock on impact. In particular, objectives may loose accuracy when exposed to even a weak physical shock. 2 Weak electromagnetic waves The power supply for this microscope system emits weak electromagnetic waves. There is a possibility that some precision electronic devices are affected by the electromagnetic waves. To prevent bad influences, locate such electronic equipment away from the microscope system. If a TV or radio reception is affected, move the TV or radio set farther from the product. 3 Dirt on the lens Scratches or fouling such as fingerprints on optical components (lenses, filters, and so on) will degrade microscope images. If these parts become dirty, clean them as described in Capter 6, "Care and Maintenance" at the end of this manual. 4 Dirt on the lamp Never touch the lamp with bare hands. Dirt or fingerprints on the lamp will result in uneven illumination and reduce the service life of the lamp. Always wear gloves when handling lamps. 5 Installation location This microscope system is a precision optical instrument. So, the usage or storage in an inappropriate environment may result in malfunctions or poor performance. Consider the following factors when selecting an installation location: Fix the microscope FN1 body securely on a baseplate or a vibration isolator with bolts. When the microscope is mounted on a microscope slider such as an XY mover, fix the microscope body on the microscope slider securely with bolts or so on to prevent the microscope from toppling over. The halogen lamp light source, the lamp house, and the optical fiber light source need to dissipate heat when the lamp is lit. So, locate such heat sources at least 10 cm away from walls. Choose a location less exposed to hazards in the event of collisions, earthquakes, or other potential disasters. Choose a sturdy vibration isolator, desk, or table for the base of the microscope system. Avoid bright locations where the product is exposed to direct sunlight or immediately under room lights. If the ambient light is bright, the microscopy image will be affected. Choose a location that is free from considerable dust or dirt. To avoid splashes, do not use the microscope system near water. Make sure the ambient temperature is 0 to 40 C and humidity is 85% or less. Using or storing the product in hot, humid locations may result in mold formation or condensation on lenses, performance degradation, or malfunctions. Select a layout that allows easy removal of the power cords from the AC inlets on the power supply and the optical fiber light source in the event of an emergency. 7

10 Notes on Handling the Microscope System 6 Focus knobs Never turn the focus knobs on the left and right sides of the product in opposite directions at the same time. Doing so may damage the product. Turning the coarse focus knob past its farthest point will damage the product. Never use excessive force when turning the knob. 7 Protect the ports (when the trinocular eyepiece tube or the variable magnification double port tube is attached). Always attach caps to any port not in use. When caps are provided with equipment, use them. If any port is not covered with a cap, extraneous light and dust may come into the equipment. 8 Handling filters (for an epi-fl attachment) Interference filters (especially excitation light filters, which are exposed to strong light) deteriorate over time. Replace the filters as necessary. Filter characteristics may alter if the filter is exposed to high humidity. To prevent changes or degradation of filter characteristics, avoid using or storing the filters under conditions of high humidity or high temperature and avoid subjecting the filters to rapid temperature changes. When a filter is not in use, store it in a desiccator or hermetically sealed container with a drying agent. The filters attached in the nine types of filter cubes listed below have sharper wavelength characteristics than standard filters. However, due to their sophisticated coatings, they must be handled with special care. In particular, take care to avoid abrasion from cleaning. Follow the procedure described in Section 1, "Lens Cleaning," of Chapter 6, "Care and Maintenance.") Single band filter cubes: DAPI, FITC, TxRed, GFP Multi band filter cubes: F-R, F-T, D-F, D-F-R, D-F-T 9 Handling the D-FLD dark-field illumination cube (for an epi-fl attachment) There are two blades in front of the D-FLD dark-field illumination cube to prevent light from coming through. Do not apply any pressure onto the blades to prevent malfunctions. 10 Contents of the package 1) The contents of the package are listed below. Check them. Microscope ECLIPSE FN1 Main body Substage part Eyepiece tube mount part Nosepiece arm part Four base auxiliary legs x1 x1 x1 x1 x4 Tools Hexagonal screwdriver Two hexagonal wrenches x1 x2 Screws Two condenser centering screws Condenser clamp screw x2 x1 8

11 Notes on Handling the Microscope System Two clamp screws for the eyepiece tube mount part front side (long type) Two clamp screws for the eyepiece tube mount part rear side (short type) x2 x2 Sixteen clamp screws for base auxiliary legs Sixteen washers for base auxiliary legs x16 x16 Sliding nosepiece x1 LWD condenser Main body x1 Two diaphragms for the diagonal illumination x2 Polarizer turret Main body x1 1/4 lambda plate positioning tool x1 Polarizer for visible lights x1 Polarizer for infrared rays x1 Variable magnification double port tube x1 Single objective holder x1 LWD 16x water immersion type objective x1 LWD 16x DIC slider x1 Variable magnification turret x1 Rectangular stage Stage main part Two auxiliary support columns Two auxiliary plates Knurled ring Acrylic chamber Chamber holder x1 x2 x2 x1 x1 x1 Eight flat head screws to clamp auxiliary plates x1 Lamp house adapter Main body Raising parts x1 x2 Precentered lamp house x1 Magnescale Main body Display part x1 x1 AC adapter x1 2) Do not discard the packing case, as it may be needed for future transportation. 9

12 Contents Contents Introduction... 1 WARNING and CAUTION Symbols Used in This Manual... 1 Meaning of the symbol used on the product... 2 Safety Precautions... 3 WARNING... 3 CAUTION... 5 Notes on Handling the Microscope System... 7 Chapter 1 Part Name Chapter 2 Microscopy Chapter 3 Operation of Each Part Coarse Focus Knob and Fine Focus Knob Focus Knob Rotation and the Objective Vertical Movement Adjustment for the Rotation Torque (rotation Resistance) of the Coarse Focus Knob Sliding Nosepiece Objective Vertical Movement Lever and the Objective Position Switching Objectives Parfocal Adjustment for the Objectives Dipping Function for a Water Immersion Type Objective Centering the Objective Attaching/Detaching the DIC Slider Single Objective Holder Eyepiece Tube Selecting the Optical Path in the Eyepiece Tube (T Eyepiece Tube, F Eyepiece Tube) Diopter Adjustment Interpupillary Distance Adjustment LWD Condenser Focusing and Centering the LWD Condenser Adjusting the Condenser Aperture Diaphragm Attaching an Optical Element into the LWD Condenser Turret Operation of the LWD Condenser for Each Microscopy Field Diaphragm Stage Water Immersion Operation (Water Immersion Type Objective Handling) Cleaning the Oil Immersion Type Objective After Use Variable Magnification Double Port Attachment Port Selection Mounting Parts of the Ports Mounting a 2/3 Inch TV Camera Aligning the Optical Paths for the Front Port and the Rear Port Parfocal Adjustment for the Front Port and the Rear Port Selecting the Magnification for the Rear Port For the DIC Microscopy

13 Contents 11 Polarizer Turret Attaching Filters For the DIC microscopy Contrast Adjustment Chapter 4 Assembly Chapter 5 Troubleshooting Viewing Problems and Control Problems Electrical System Problems Chapter 6 Care and Maintenance Chapter 7 Specifications

14 1 Part Name If the microscope is not assembled yet, refer to Chapter 4, "Assembly" and assemble the microscope beforehand. For details about assembling and handling the power supply and the light source, see the instruction manuals for them. 1. Main part of the microscope Diopter adjustment ring Eyepiece Optical path selector lever Eyepiece tube Magnification selector dial Variable magnification double port attachment * Epi-fl attachment Slide click release button Objective vertical movement lever Sliding nosepiece Sliding nosepiece lever Rectangular stage Chamber Condenser Condenser focus knob Condenser centering screw Base auxiliary leg This photo depicts the ECLIPSE FN1 microscope configured with the epi-fl attachment, the variable magnification double port attachment, and the ergonomic eyepiece tube. * The product name for the variable magnification double port attachment is "Mag. Variable Double Port." * The ultraviolet light shield is removed from the microscope in the photo above. The following figure shows the ultraviolet light shield. Ultraviolet light shield 12

15 Chapter 1 Part Name Focus adjustment ring for the rear port Analyzer selector lever 2 Lamp house Parfocal correction knob Objective Coarse focus torque adjustment ring Rear side coarse focus knob Rear side fine focus knob Stage movement knob for the Y-axis Stage movement knob for the X-axis Front side coarse focus knob Field diaphragm control Front side fine focus knob 2. TE2-PS100W power supply WARNING The bottom of the power supply becomes extremely hot while it is in use. Do not obstruct the air vents on the sides of the power supply. Brightness control knob Power indicator This lamp is lit when power is on. OUTPUT connector This connector is used for the lamp. It is connected to the lamp house through the lamp cable. Power switch Push it to the "I" side to turn on. Push it to the "O" side to turn off. AC inlet Connect the specified power cord that meets the voltage provided. Refer to Chapter 7, "Specification" for the specified power cord. Sanwa Electric SNS-1603-RSFb Pin 1: Output + Pin 2: Output - Pin 3: Not used 13

16 2 Microscopy The general procedure for the microscopy with water immersion objective is described below. For details, refer to Chapter 3, "Operation of Each Part." 1 Turn on the power supply or the optical fiber light source. (Push the switch to the "I" postion.) 2 Adjust the brightness with the brightness control knob on the power supply or on the optical fiber light source. 3 Rotate the polarizer turret to locate the desired filter into the optical path. Under the standard configuration, the following filters are attached to the polarizer turret. Upper turret: NCB11 Lower turret: ND filters (ND2, ND4, and ND16) 4 Push in the sliding nosepiece to locate the near side objective, the low magnification objective (4x or 10x), into the optical path. Push in CAUTION: Make sure to move the objective vertical movement lever to the center position when you change objectives between the near side one and the far side one. You cannot change objectives when the objective vertical movement lever is placed at the right or the left. If you attempt to change objectives forcibly, the microscopy may be damaged. Refer to Section 2.2 in Chapter 3, "Selecting an Objective." 5 Place the specimen on the stage. 6 Rotate the condenser focus knob to raise the LWDcondenser. The LWD condenser tip must be raised to the limit position where it does not touch the specimen bottom. 7 Fully open the field diaphragm with the field diaphragm control. And fully open the condenser aperture diaphragm with the condenser aperture diaphragm lever. Condenser aperture diaphragm lever Condenser focus knob 8 Move the stage to place the target part of the specimen into the optical path. 14 Field diaphragm control

17 Chapter 2 9 Microscopy When the trinocular eyepiece tube is attached, push in the optical path selector lever and select the optical path to make 100% light go into the binocular part. 2 Refer to Section 4.1 in Chapter 3, "Selecting the Optical Path in the Eyepiece Tube (T Eyepiece Tube, F Eyepiece Tube)." 10 Focus on the specimen with the coarse focus knob and the fine focus knob. 11 Adjust the diopters and the interpupillary distance. Check for your both eyes. Refer to Section 4.2, "Diopter Adjustment" and 4.3, "Interpupillary Distance Adjustment" in Chapter 3. Push in Adjust the diopters. Adjust the interpupillary distance. 12 Focus and center the LWD condenser. Refer to Section 5.1 in Chapter 3, "Focusing and Centering the Condenser." Condenser centering screw 13 Move the objective vertical movement lever to the center to retract the low magnification objective into the upper position. 14 Pull the sliding nosepiece to the front to locate the water immersion type objective into the optical path. CAUTION: Make sure to move the objective vertical movement lever to the center position when you change objectives between the near side one and the far side one. You cannot change objectives when the objective vertical movement lever is placed at the right or the left. If you attempt to change objectives forcibly, the microscopy may be damaged. 15 Move the objective vertical movement lever to the center. Pull

18 Chapter 2 Microscopy 15 Move the objective vertical movement lever to the left so that the objective tip touches the surface of the water over the specimen. 16 To perform the parfocal correction, use the parfocal correction knob on the sliding nosepiece. With this focusing operation, a confocal position for the two objectives can be attained. Parfocal correction knobs 17 Adjust the brightness with the brightness control knob on the power supply or on the optical fiber light source. 18 Stop down the field diaphragm with the field diaphragm control so that it circumscribes the viewfield. Condenser aperture diaphragm lever Refer to Section 6 in Chapter 3, "Field Diaphragm." Stop down the condenser aperture diaphragm with the condenser aperture diaphragm lever to about 70 to 80% of the numerical aperture of the objective. Field diaphragm control Start the microscopy. 16

19 3 Operation of Each Part 1 Coarse Focus Knob and Fine Focus Knob 1.1 Focus Knob Rotation and the Objective Vertical Movement The rear side fine focus knob is marked in 1 µm increments. The objective rises/falls approximately 0.1 mm per one turn of the fine focus knob. The objective rises/falls approximately 14 mm per one turn of the coarse focus knob. The vertical movable range (coarse/fine focus stroke) of the objective is 15 mm. It can be risen 13 mm above from the focal point and can be lowered 2 mm below from the focal point. Focus knob rotation and the objective vertical movement Never attempt either of the following actions, as they will damage the microscope. Rotating the left and right focus knobs in opposite directions at the same time. Rotating the coarse focus knob past the stopper. 1.2 Adjustment for the Rotation Torque (rotation Resistance) of the Coarse Focus Knob The torque of the coarse focus knob can be adjusted. To increase the torque, rotate the torque adjustment ring at the base of the rear side coarse focus knob in the counterclockwise direction. To decrease the torque, rotate the ring in the clockwise direction. CAUTION: If the torque is adjusted very heavy and the coarse focus knob is rotated, the microscope may be damaged. Decrease the torque Increase the torque Coarse torque adjustment ring 17

20 Chapter 3 Operation of Each Part 2 Sliding Nosepiece 2 Sliding Nosepiece The sliding nosepiece can be attached two objectives into the near side socket and the far side socket. The objectives are changed by sliding the nosepiece to and from. Sliding nosepiece 2.1 Objective Vertical Movement Lever and the Objective Position You can move the objective upward and downward by operating the objective vertical movement lever on the sliding nosepiece from side to side. This operation is independent of the focus knob operation. Objective vertical movement lever This operation is useful for retracting the objectives to switch objectives and for dipping the objective into the water to perform the water immersion. You can operate the lever only when the objective is placed in the optical path. The vertical movable range of the objective is up to 15 mm above the focal point. With this movable range, you can observe a specimen in a high petri dish. The table below shows the relationship between the objective vertical movement lever position and the objective position. Lever position Right Near side objective position (The near side objective is located in the optical path.) Far side objective position (The far side objective is located in the optical path.) Observation position Center Retracted position Retracted position Left Observation position 18

21 Chapter 3 2 Operation of Each Part Sliding Nosepiece Switching Objectives 2.2 In this section, an example case is explained. (Changing objectives from the near side to the far side) CAUTION: You cannot change objectives when the objective vertical movement lever is placed at the right or the left. If you attempt to change objectives forcibly, the microscopy may be damaged. Make sure to move the objective vertical movement lever to the center position when you change objectives between the near side one and the far side one. 1 Move the objective vertical movement lever from the left to the center. The near side objective is retracted. Move the lever to the center. 2 With the near side objective retracted, push the slide click release button and pull the sliding nosepiece lever fully to the near side. Sliding nosepiece lever Pull Slide click release button 3 Release the slide click release button. Move the sliding nosepiece slightly to and from to find a click-stop position and fit in it. 4 Gently move the objective vertical movement lever from the center to the right. The far side objective will be placed in the observation position. Move the lever to the right. 19 3

22 Chapter Operation of Each Part Sliding Nosepiece Parfocal Adjustment for the Objectives Usually, the focal length for a dry type objective is different from that of a liquid immersion type objective. If you change objectives between these types with no correction, the image will go out of focus. In this case, adjust the parfocal correction knobs on the sides of the sliding nosepiece. Parfocal correction knob Near side objective Far side objective To perform the parfocal correction adjustment, move the objective vertical movement lever to the left or the right. The parfocal correction knob on the left side is used for the near side objective, and the parfocal correction knob on the right side is used for the far side objective. Turn the knob in a clockwise direction to raise the objective (to raise the focal position). 2.4 Dipping Function for a Water Immersion Type Objective Push the objective vertical movement lever on the nosepiece farther than the normal position at the left or the right. The objective moves down 1 mm. When the lever is released, the lever and the objective come back to their original positions. Push the lever as far as it will go. CAUTION: To prevent damage to the specimen and the objective, be careful not to perform the dipping function accidentally. 2.5 Centering the Objective When you wish to decrease the difference between the two center positions of the near side objective and the far side objective, center the objectives. Rotate the three M3 hexagonal socket head set-screws. Adjust the center positions of the near side objective and the far side objective so that they come together. To adjust the center positions more accurately, use an eyepiece with crosshairs. When you wish to perform a microscopy of a small specimen such as a patch clamp method, you should center the objectives beforehand. 20 Centering screws

23 Chapter Operation of Each Part Single Objective Holder Attaching/Detaching the DIC Slider To attach/detach the DIC slider for the DIC microscopy, lower the objective. The DIC sliders are inserted into the DIC slider slots on the front right side of the both objectives. 3 DIC slider 3 Single Objective Holder Single objective holder The single objective holder is used only for the LWD 16x water immersion type objective. The DIC slider for the DIC microscopy is inserted into the DIC slider slot on the upper side of the objective clamp screw. DIC slider Eyepiece Tube Selecting the Optical Path in the Eyepiece Tube (T Eyepiece Tube, F Eyepiece Tube) The optical path selector lever can be used to select the way to distribute light to the binocular part and the vertical tube. 1 Distribution of light Binocular part: vertical tube BINO & PHOTO PHOTO Lever position BINO 1 2 Distribution of light Binocular part: vertical tube Lever position BINO 1 3 T eyepiece tube 2 F eyepiece tube 21 PHOTO

24 Chapter 3 Operation of Each Part 4 Eyepiece Tube (1) Vertical tube adapter A photo adapter is attached as a standard accessory for photomicrographic equipment. To use this adapter, insert it into the vertical tube of the eyepiece tube and fix it with three screws on the vertical tube. If you wish to use a TV camera, you must attach an optional TV adapter into the vertical tube. (2) Disabling the Clicking of the Optical Path Selector Lever on the T Eyepiece Tube and the F Eyepiece Tube There is a "NO CLICK" switch on the eyepiece tube mount part. When this switch is slid in the direction of the arrow, the clicking of the optical path selector lever is disabled. So, if you need to eliminate slight vibrations resulting from the clicking action, slide this switch with a narrow piece of metal such as the hexagonal screwdriver provided. NO CLICK 4.2 Diopter Adjustment Diopter adjustment compensates for differences in visual acuity between the right and left eyes, improving binocular observation. It's hard to adjust the diopters with the standard procedure because of the sliding nosepiece. So, adjust the diopters as described below. 1 Turn the diopter adjustment rings on both eyepieces to align their engraved lines with the edges of the outer tubes of the eyepieces. (They are the standard positions for the diopter rings.) Engraved line 2 3 Diopter adjustment standard position Place the low magnification objective into the optical path. Observe the specimen with both eyes and focus on the specimen surface by using the focus knob. Observe the specimen with your right eye only and focus on the specimen surface by using the diopter adjustment ring. Do the same for your left eye. * When an eyepiece with a reticle for photomicrography is used, adjust the diopters as described below. 1 Adjust the diopter adjustment ring to the standard position. And place the low magnification objective into the optical path. 22

25 Chapter 3 Operation of Each Part 5 LWD Condenser 2 3 Observe the specimen with both eyes and focus on the specimen surface by using the focus knob. Rotate the right diopter adjustment ring while observing the specimen with your right eye so that the reticle and the specimen come into focus. Do the same for your left eye. 4.3 Interpupillary Distance Adjustment Before adjusting the interpupillary distance, focus on the specimen with a low magnification objective by following the steps of 1 to 10 in Section 2 of Chapter 1, "Microscopy." 3 Adjust the interpupillary distance so that the viewfields for both eyes come together. Doing so will make observation through the binocular eyepieces with both eyes easier. Converge until the right and left view fields coincide. 5 LWD Condenser 5.1 Focusing and Centering the LWD Condenser The LWD condenser must be focused and centered so that the light passing through the LWD condenser forms an image at the correct location (the center of the optical path) on the specimen surface. Before focusing and centering the LWD condenser, focus on the specimen with a low magnification objective by following the steps of 1 to 11 in Section 2 of Chapter 1, "Microscopy." When you wish to use the 4x objective with the LWD condenser, attach the additional lens for 4x magnification into the turret of the LWD condenser and place it into the optical path. When you wish to use an objective of a magnification higher than the 4x magnification, you need not attach the additional lens. 1 Rotate the field diaphragm control to fully stop down the diaphragm. 2 3 Turn the condenser focus knob to form the field diaphragm image on the specimen image. Turn the condenser centering screws so that the field diaphragm image is roughly positioned in the center of the view field. Condenser centering screws Condenser focus knob Field diaphragm control 23

26 Chapter 3 Operation of Each Part 5 LWD Condenser 4 Place an objective of 40x or 80x into the optical path. Rotate the parfocal correction knob on the sliding nosepiece to focus on the specimen. Parfocal correction knobs Near side objective Far side objective 5 6 Turn the condenser focus knob to form the field diaphragm image on the specimen surface. Turn the condenser centering screws so that the field diaphragm image is positioned in the center of the view field. This is easiest if you set the field diaphragm aperture to slightly smaller than the eyepiece view field. Field diaphragm image Field diaphragm image Eyepiece viewfield Eyepiece viewfield 5.2 Adjusting the Condenser Aperture Diaphragm The aperture diaphragm is important because it is related to the resolution, contrast, depth of focus, and brightness of the optical image. Turning the condenser aperture diaphragm lever on the LWD condenser changes the size of the aperture diaphragm. As the aperture diaphragm is stopped down, resolution and brightness are reduced while contrast and depth of focus are increased. Conversely, as the aperture diaphragm is opened, resolution and brightness are increased while contrast and depth of focus are reduced. It is not possible to adjust one pair of characteristics without affecting the other. Generally, a satisfactory image with appropriate contrast can be obtained with an aperture setting that is 70% to 80% of the numerical aperture of the objective. The numerical aperture is indicated on the barrel of each objective. Example: when the numerical aperture of an objective is 0.80, 0.8 x 0.7 to 0.8 = 0.56 to 0.64 If the aperture diaphragm is stopped down too far, the resolution is reduced; therefore, except when viewing a nearly transparent specimen, we do not recommend stopping down the aperture to less than 60% of the numerical aperture of the objective. Condenser aperture diaphragm lever Fluor 40x / 0.80 W DIC M /0 WD 2.0 "40x/ 0.80" means that the magnification is x40 and the numerical aperture is Fluor 40X/0.80W DIC M /0 WD

27 Chapter 3 Operation of Each Part 5 LWD Condenser (1) Aperture diaphragm adjustment with the pupil of the objective Remove one of the eyepieces and look into the sleeve. You can see the pupil of the objective (that is a bright area when the aperture diaphragm is fully opened.) Stop down the condenser aperture diaphragm so that the diaphragm aperture is adjusted to about 70% to 80% of the pupil of the objective. Pupil of the objective 70~ Aperture diaphragm 3 (2) Aperture diaphragm adjustment with an optional centering telescope Observe an enlarged view of the pupil of the objective with an optional centering telescope and adjust the aperture diaphragm of the LWD condenser Remove one of the eyepieces. And then, attach an optional centering telescope with an optional adapter in place. Fully stop down the aperture diaphragm with the condenser aperture diaphragm lever. Turn the eyepiece section of the centering telescope with holding the flange of the centering telescope to focus on the aperture diaphragm image. Turn the diaphragm control lever to adjust the aperture diaphragm. (This is normally adjusted to 70-80% of the pupil of the objective.) Remove the centering telescope with the adapter and attach the eyepiece to its original position. 5.3 Attaching an Optical Element into the LWD Condenser Turret You can perform the bright-field microscopy under the diascopic illumination (with 4x objective to 100x objective), the differential interference contrast microscopy under the diascopic illumination (with visible lights or with infra-red lights), and the microscopy under the diagonal illumination when you use the LWD condenser. And you can attach an optical element into the condenser turret of the LWD condenser for each microscopy Remove the two button bolts on the condenser turret of the LWD condenser and remove the cover. Attach a desired optical element in place and fix it. Put the cover back to its original position. Fix the cover with the button bolts. Fix the element. Attach an optical element. 25

28 Chapter 3 Operation of Each Part 5 LWD Condenser 5.4 Operation of the LWD Condenser for Each Microscopy (1) For the bright-field microscopy Place the vacant hole of the turret into the optical path. (2) For the bright-field microscopy with the 4x objective Attach the additional lens for 4x magnification into the DIC prism N2 position of the condenser turret of the LWD condenser and fix it with a set-screw. And then, place it into the optical path. (* There is no indication about the 4x magnification on the condenser turret.) When you wish to use an objective with a larger magnification than the 4x objective, you need not attach the additional lens. (3) For the DIC microscopy Attach an objective for the DIC microscopy. Attach a DIC prism into the condenser turret of the LWD condenser that meets the specification of the objective. You can see the DIC prism type on the side of the objective. (4) For the microscopy under the diagonal illumination You can observe a nearly transparent specimen by improving the contrast under the diagonal illumination. And, you can rotate the slit for the diagonal illumination around the optical center to change the orientation of the shade of the specimen Attach a slit for the diagonal illumination into the condenser turret of the LWD condenser. Rotate the turret and place the slit into the optical path. Rotate the slit orientation ring on the turret to adjust the illumination light orientation. Slit orientation ring 26

29 Chapter 3 Operation of Each Part 6 Field Diaphragm 6 Field Diaphragm The field diaphragm restricts the illumination light to the area on the specimen to be observed. Operating the field diaphragm ring changes the size of the field diaphragm. For normal observation, the size of the diaphragm should be such that it is just outside the edge of the viewfield. If a broader area than necessary is illuminated, stray light will enter the optical system, creating flaring, reducing the contrast of the optical image. The operation of the field diaphragm is especially important in photomicrography; generally, the best results are obtained by stopping down the field diaphragm to just slightly larger than the image area or the area that will be reproduced on the film, that is, the size of the picture composition frame (for a digital camera, the size of the CCD). Field diaphragm control 3 7 Stage You can adjust the rotation torques (rotation resistances) of the stage movement knobs for the X-axis and for the Y-axis. To adjust them, move the stage to the edge of the movement. It makes the adjustment easy. Torque adjustment for the Y-axis To increase the torque, rotate the knob B in the arrow a direction while holding the knob A. To decrease the torque, rotate the knob B in the opposite direction while holding the knob A. Torque adjustment for the X-axis To increase the torque, rotate the knob C in the arrow b direction while holding the knob D. To decrease the torque, rotate the knob C in the opposite direction while holding the knob D. When you hold the knob D, put your finger on the lower hole of the knob D. Increase the torque a Decrease the torque b Decrease the torque Knob A Knob B (Y-axis) Knob C (X-axis) Increase the torque Knob D 27

30 Chapter 3 Operation of Each Part 8 Water Immersion Operation (Water Immersion Type Objective Handling) 8 Water Immersion Operation (Water Immersion Type Objective Handling) An objective with a "W" mark is a water immersion type objective. It is designed to focus on a specimen at a working distance in water. To use this type of objective, apply water (normal saline solution or such) into a chamber. And lower the objective to make its tip touch the water. When you wish to observe a specimen to a depth of 0.2 mm or so on from the surface, for example a sliced brain, you are recommended to perform an observation under infrared rays or to use a water immersion type objective with a correction ring, Plan 100xW. CAUTION: Leaking water from the chamber can give rise to corrosion of the stage or the condenser lens. If water in the chamber spills on the stage or on the condenser lens, it should be wiped out soon. After use, wipe off water from the tip of the objective, then follow up by wiping with absolute alcohol. If you observe water stains, apply a small amount of neutral detergent and wipe gently, then follow up with absolute alcohol. 9 Cleaning the Oil Immersion Type Objective After Use To wipe off immersion oil, use petroleum benzine. For optimum results, we recommend following up petroleum benzine with absolute alcohol (ethyl or methyl alcohol). If petroleum benzine is unavailable, use methyl alcohol. However, methyl alcohol does not clean as well as petroleum benzine, it will be necessary to wipe the surface repeatedly. (Usually, three- or four-times wipe is sufficient to clean the lenses.) WARNING: When using petroleum benzine or absolute alcohol, always follow the instructions provided by the manufacturer. These liquids are highly flammable and must be kept away from flames and sparks. 28

31 Chapter 3 Operation of Each Part 10 Variable Magnification Double Port Attachment 10 Variable Magnification Double Port Attachment You can attach an eyepiece tube onto the front port of the variable magnification double port attachment and a 2/3 inch TV camera onto the rear port of the variable magnification double port attachment. Front port Rear port You can select the port condition between the simultaneous usage of both ports and the front port usage only. 3 When the simultaneous usage of both ports is selected, 100% of visible lights goes through the front port and 100% of infrared rays goes through the rear port. The magnification for the rear port can be selected from among x0.35, x2, and x4. With the rear port, the water immersion type objective of x16 and the numerical aperture 0.80 enables various observations from a low magnification with wide view field to a high magnification with high resolution without changing objectives. Take note of that vignettings may occur when the magnification for the rear port is x0.35. This is not a malfunction. The front port can be used for the following observations: Bright-field microscopy Microscopy under the diagonal illumination DIC microscopy (combined with the FN-IR/ISA DIC IR analyzer or the D-FL U-EPI FL epi-fl attachment) Fluorescent microscopy (combined with the J-FL epi-fl attachment) The rear port can be used for the following observations: Infrared bright-field microscopy Infrared microscopy under the diagonal illumination Infrared DIC microscopy (for DIC observations under infrared rays) CAUTION: When the J-FL epi-fl attachment and the variable magnification double port attachment are used together, the indications for the filter cassette type and the turret number on the J-FL epi-fl attachment are hidden. 29

32 Chapter 3 Operation of Each Part 10 Variable Magnification Double Port Attachment 10.1 Port Selection To use the front port and the rear port simultaneously, Rotate the port selector knob 180 degree in a counterclockwise direction. To use the front port only, Rotate the port selector knob 180 degree in a clockwise direction. Port selector knob 10.2 Mounting Parts of the Ports The front port is a dovetail joint of 46 mm in diameter. This port is used for an eyepiece tube or a variable magnification turret. * The rear port is a C mount unit. This port is used for a TV camera. When the two M4 set screws at the C mount unit screw part are loosened, the C mount unit can be removed. When the C mount unit is removed, the rear port can be used as an ISO mount of 38 mm in its inner diameter and of 29 mm distance from the hardware edge to the imaging position.the product name for the variable magnification turret is "Mag. Variable Turret." M4 set screws at the C mount unit screw part 10.3 Mounting a 2/3 Inch TV Camera When the M4 set screw on the knurled ring of the rear port is tighten and the two M4 set screws at the C mount unit screw part are loosen, the C mount unit can be rotated freely. A 2/3 inch TV camera can be mounted onto the rear port in this condition. M4 set screw on the knurled ring After mounting the TV camera, loosen the M4 set screw on the knurled ring and tighten the M4 set screw at the C mount unit screw part. M4 set screws at the C mount unit screw part 30

33 Chapter Operation of Each Part Variable Magnification Double Port Attachment Aligning the Optical Paths for the Front Port and the Rear Port To align the optical paths for the front port and the rear port, adjust the rear port position. Set screws Rotate the two M4 set screws to adjust the rear port position (for the 2/3 inch TV camera). After the adjustment, tighten the M4 set screw on the back of the tube Parfocal Adjustment for the Front Port and the Rear Port You can adjust the focus position for the rear port so that the focus positions for the front port and the rear port come together. M4 set screw on the knurled ring B A Check that the M4 set screw on the knurled ring on the rear port is loosened and that the M4 set screws at the C mount unit screw part is tightened. To raise the camera position on the rear port, rotate the knurled ring in the "A" direction. To lower the camera position, rotate the knurled ring in the "B" direction. During the adjustment, watch the monitor image M4 set screws at the C mount unit screw part Selecting the Magnification for the Rear Port You can change the magnification of the rear port by rotating the magnification selector dial on the right side of the tube in the sequence of 0.35x, 2x, and 4x. You cannot change the magnifications between 0.35x and 4x directly. 31 Magnification selector dial

34 Chapter 3 Operation of Each Part 11 Polarizer Turret 10.7 For the DIC Microscopy (1) To perform the infrared DIC microscopy through the rear port Push in the lever on the left side of the variable magnification double port attachment to locate the IR analyzer into the optical path. When you wish to use the epi-fl attachment together, locate the empty hole into the optical path for the epi-fl attachment turret and check that the analyzer in the epi-fl attachment turret is not used.. Push in (2) To perform the infrared DIC microscopy through the rear port You must use the simple analyzer, FN-IR/ISA DIC IR analyzer, or the epi-fl attachment, D-FL U-EPI FL, to perform the DIC microscopy through the front port. When you use the epi-fl attachment, insert the DIC analyzer. Push in 11 Polarizer Turret You can perform the DIC microscopy under visible lights (the wavelength range is from 430 nm to 770 nm) and under infrared rays (the wavelength range is 850 nm to 950 nm) with the microscope FN1. To perform the DIC microscopy, attach a polarizer for visible lights or infrared rays (the FN-P polarizer or the FN-IRP polarizer) into the upper turret of the polarizer turret. 32

35 Chapter Operation of Each Part Polarizer Turret Attaching Filters The polarizer turret consists of the upper turret and the lower turret. NCB filter In the standard configuration, three types of ND filters are attached (ND2, ND4, and ND16) in the lower turret. One position of the turret is an empty hole position. 3 An optional filter of 33 mm diameter can be attached into the empty hole position of the upper turret. In the standard configuration, an NCB filter is attached. Empty hole position 11.2 For the DIC microscopy In this section, an example case is explained. (The DIC microscopy under the infrared rays is described.) (1) Preparations When the variable magnification double port attachment is used, Locate the analyzer into the optical path of the rear port. Remove the DIC prism of the single objective holder from the optical path. And remove the DIC prism of the condenser lens from the optical path. When the variable magnification double port attachment and the epi-fl attachment (D-FL UEPI FL) are used, Locate the empty hole into the optical path for the epi-fl attachment turret and check that the analyzer in the epi-fl attachment turret is not used. Remove the DIC prism of the single objective holder from the optical path. And remove the DIC prism of the condenser lens from the optical path. When the trinocular eyepiece tube and the analyzer (FN-IR/ISA DIC IR analyzer) are used or when the epi-fl attachment (D-FL U-EPI FL) is used, Locate the IR analyzer into the optical path. Remove the DIC prism of the sliding nosepiece from the optical path. And remove the DIC prism of the condenser lens from the optical path. 33

36 Chapter 3 Operation of Each Part 11 Polarizer Turret (2) Attaching the IR Polarizer 1 Separate the upper part (band pass filter and 1/4 lambda plate) and the lower part of the IR polarizer. Upper part (Band pass filter and 1/4 lambda plate) Lower part 2 Insert the adjustment tool for the 1/4 lambda plate orientation, which is provided with the polarizer turret, into the lower part of the IR polarizer. In doing so, adjust the notch position on the adjustment tool for the 1/4 lambda plate and the protrusion position on the lower part of the IR polarizer. Adjustment tool for the 1/4 lambda plate orientation Notch Protrusion Lower part of the IR polarizer 3 Rotate the knurled ring on the right side of the polarizer turret to adjust the index (a white point) to the center line of the scale. Insert the IR polarizer assembled in Step 2 into the upper turret of the polarizer turret. The notch of the IR polarizer must be oriented in the way as shown in the figure. Notch Clamp screw Knurled ring Align the white point and the center line. 4 5 Attach the polarizer turret into the microscope body. Rotate the knurled ring on the right side of the polarizer turret and search the position where the view becomes darkest. After the adjustment, tighten the clamp screw. 34

37 Chapter 3 Operation of Each Part 11 Polarizer Turret 6 Remove the 1/4 lambda plate and insert the upper part of the IR polarizer (the band pass filter and the 1/4 lambda plate) Contrast Adjustment 1 2 Insert the DIC slider into the single objective holder or into the sliding nosepiece to locate the DIC prism of the condenser into the optical path. Rotate the knurled ring on the right side of the polarizer turret upper side to change the polarizer orientation and adjust the contrast. 3 35

38 4 Assembly WARNING To prevent electrical shock or fire, turn off the power switch of the power supply (flip the switch to the "O" side) during assembly. The electrical power should be supplied after assembling the microscope system. CAUTION When assembling the microscope, assemble the eyepiece tube first. To carry the microscope, first detach the lamp house. Tilt the microscope body while supporting it and then securely hold it by the base. Never grasp the focus knobs, the eyepiece tube, the sliding nosepiece, the stage, or so on when carrying the microscope system. This may cause the part to come off, resulting in damage to the microscope system. Be careful not to pinch your hands or fingers when setting up the microscope. Scratches or fingerprints on the lenses will adversely affect the image. Be careful not to scratch or touch the lens surfaces. This microscope system is a precision optical instrument. Handle it carefully and do not subject it to a strong physical shock. (In particular, objectives may loose accuracy when exposed to even a weak physical shock.) Required tools Hexagonal screwdriver (provided): For M4 set screws Two hexagonal wrenches (provided): For M4 hexagonal socket head bolts and M5 hexagonal socket head bolts Phillips screwdriver For M3 Phillips head screw When not using these tools, store them together in a safe place for a future use. Installation location This microscope system is a precision optical instrument. So, the usage or storage in an inappropriate environment may result in malfunctions or poor performance. Consider the following factors when selecting an installation location: Avoid bright locations where the product is exposed to direct sunlight or immediately under room lights. If there is excessive ambient light, the image quality deteriorates. Choose a location that is free from considerable dust or dirt. Choose a flat surface with little vibration. Choose a sturdy desk or table for the base of the microscopy system. Do not install the microscope system in a hot or humid location. Using or storing the product in a hot or humid location may result in mold formation or condensation on lenses, performance degradation, or malfunctions. 36

39 Chapter 4 Assembly Configuration diagram C-mount TV camera C-mount TV adapter TV tube Trinocular Tube Trinocular Tube Ergonomic Eyepiece Tube C-TEP DSC Port Mag. Variable Double Port Mag. Variable Turret Simple Polarizing Attachment DIC/IR Analyzer High-intensity Light Source FL/DIC Analyzer 1 Epi-fl Attachment Motorized Shutter Mercury Lamp Fiber Light Source DIC Slider Sliding Nosepiece 1 Magnet Scale Objectives Stage Slider CFI75 Holder YM-EPI 3-3PIN Extention Cable Power Supply Objective (CFI75 LWD) FN-LPA Lamp House Adapter FN-LH Precentered Lamp House 12V100W Halogen Lamp Microscope FN1 Light Guide Optical Fiber Light Source DIC Module LWD Condenser XY Mover Support Lens Polarizer Polarizer Turret 37

40 Chapter 4 Assembly 1 Installing/removing the lamp and the lamp house (replacing lamps) CAUTION To prevent electrical shock and malfunctions, always turn off the power switched for the power supply and optical fiber light source (press them to the "O" position), and unplug the power cords from the wall outlet when installing/removing the lamp house. To prevent burn injury, allow the lamp and the lamp house to cool down sufficiently (for at least 30 minutes after the lamp is turned off), before replacing lamps. Use the Nikon FN-LH model for the lamp house. To attach the FN-LH lamp house onto the FN1 microscope body, the FN-LPA lamp house adapter (optional) is required. Use the specified cable (YM-EPI, 3-3PIN extension cable) to connect the lamp house and the power supply. Use a 12V 100W halogen lamp (the PHILIPS 7724 lamp or the OSRAM HLX64623 lamp). Be careful not to touch the glass surface of the lamp with bare hands. Doing so will cause fingerprints, grease, etc. to burn onto the lamp surface, reducing the illumination. If you do get any fingerprints or dirt on the lamp, wipe them clean. Make sure the lamp house cover is securely fitted to the lamp house after replacing lamps. Never use the lamp house while its cover is off. Attaching the lamp house In this section, an example case is explained. (The optical fiber light source has been connected already.) (1) Check that the power switches on the power supply and the optical fiber light source are turned off (the switches are pushed to the "O" side). (2) Loosen the three M3 hexagonal socket head bolts at the standard optical fiber adapter on the rear side of the microscope body and remove the standard optical fiber adapter. M3 hexagonal socket head bolts 38

41 Chapter 4 Assembly (3) Separate the vertical tube part, which includes a lens, and the lamp house connector part of the lamp house adapter. Insert the vertical tube part only in place of the optical fiber adapter. And then, fix the vertical tube part with three M3 hexagonal socket head bolts. M3 hexagonal socket head bolts (4) Attach the lamp house connector part onto the vertical tube part and fix them with four M3 hexagonal socket head bolts. M3 hexagonal socket head bolts (5) Insert the lamp house into the lamp house connector part and fix it with the clamp screw on the upper side of the lamp house. Clamp screw Replacing lamps * For information about replacing lamps for the LS-DWL-N halogen light source for the optical fiber illumination, refer to the instruction manual provided with the light source. (1) Check that the power switches on the power supply and the optical fiber light source are turned off (the switches are pushed to the "O" side). (2) Use the hexagonal wrench to loosen the lamp house clamp screw on the upper side of the lamp house, then remove the lamp house cover. Lamp house cover clamp screw 39

42 Chapter 4 Assembly (3) While pressing the lamp clamp lever, push the lamp into the socket pin holes as far as it will go. (Don't touch the glass surface of the lamp with bare hands.) Lamp socket (4) Return the lamp clamp lever to its original position. Make sure that the lamp is not tilted. (5) Attach the lamp house cover securely and tighten the clamp screw. Make sure that the cover is fixed in place and will not come off even when shaken by hand. 2 Assembling the nosepiece arm (1) Loosen the two nosepiece arm clamp screws on the right side of the microscope mainstay and remove the cover. (2) Attach the nosepiece arm onto the dovetail on the microscope mainstay. (3) Tighten the nosepiece arm clamp screw on the right side of the microscope mainstay. (4) Reattach the cover on the right side of the microscope mainstay. Attach the arm from above Nosepiece arm Nosepiece arm clamp screws 3 Assembling the eyepiece tube mount part Attach the eyepiece tube mount part onto the nosepiece arm and fix it with four bolts on the upper part of the microscope mainstay. Use two M5-35 bolts for the front side and two M5-30 bolts for the rear side. Clamp bolts 40

43 Chapter 4 Assembly 4 Installing the Epi-fl attachment/variable magnification double port attachment/simple DIC analyzer 1 Epi-fl attachment (1) Remove the ultraviolet light shield and the button bolts at the mounting part. (2) Fix the epi-fl attachment on the eyepiece tube mount part of the microscope main body with an M4 hexagonal socket head set screw. (3) Attach the single objective holder or the sliding nosepiece. And then, attach the ultraviolet light shield to the front of the eyepiece tube mounting part of the microscope. 2 Variable magnification double port attachment M4 hexagonal socket head set screw Attach the single objective holder or the sliding nosepiece. And then, attach the ultraviolet light shield. M4 hexagonal socket head bolts Fix the variable magnification double port attachment on the eyepiece tube mount part of the microscope main body with an M4 hexagonal socket head set screw. To use the epi-fl attachment and the variable magnification double port attachment together, attach the variable magnification double port attachment onto the eyepiece tube mount part of the epi-fl attachment and fix the double port attachment with an M4 hexagonal socket head set screw. Then, fix the epi-fl attachment with two M4 hexagonal socket head bolts onto the microscope main body. The epi-fl attachment is put between the microscope main body and the variable magnification double port attachment. M4 hexagonal socket head set screw 3 Simple DIC analyzer Fix the simple DIC analyzer on the eyepiece tube mount part of the microscope main body with an M4 hexagonal socket head set screw. To use the simple DIC analyzer with an epi-fl attachment, attach the simple DIC analyzer onto the epi-fl attachment and fix the analyzer with an M4 hexagonal socket head set screw. CAUTION: When an epi-fl attachment with an analyzer slot is used for this purpose, do not use the analyzer of the epi-fl attachment. 41

44 Chapter 4 Assembly 5 Attaching an eyepiece tube Attach an eyepiece tube on the eyepiece tube mount part of the microscope main body and fix it with an M4 hexagonal socket head set screw. When using an epi-fl attachment, the variable magnification double port attachment, or the simple DIC analyzer, attach the eyepiece tube onto the eyepiece tube mount part of the upper part of the epi-fl attachment, the variable magnification double port attachment, or the simple DIC analyzer, and fix the eyepiece tube with an M4 hexagonal socket head set screw. M4 hexagonal socket head set screw 6 Attaching eyepieces Attach eyepieces of the same magnification. There is a positioning protrusion on the right sleeve. Align the notch of the eyepiece with the protrusion on the sleeve and slide in the eyepiece. Some types of eyepiece tube do not have such protrusions. If necessary, attach optional rubber eye-guards into the grooves around the eyepieces. 7 Assembling the stage unit and attaching the LWD condenser 1 Attaching the stage onto the substage M5 hexagonal socket head bolts (1) Remove the four M5 hexagonal socket head bolts on the upper part of the substage. (2) Attach the stage onto the substage. Secure the stage with the four M5 hexagonal socket head bolts removed in Step (1). 2 Attaching the stage unit onto the microscope Take the stage unit assembled in the previous steps, Attaching the stage onto the substage. Insert the stage unit into the dovetail of the microscope from the front side to the limit. And then, fix it with two M4 hexagonal socket head set screws. M4 hexagonal socket head set screws 42

45 Chapter 4 3 Assembly Attaching the auxiliary stage support columns (1) Move the stage left and right and around so that you can access the mounting hole for the auxiliary stage support column. (2) Hold an auxiliary stage support column and bring it to the stage from below. And then, align its screw hole and the mounting hole. (3) Insert a bolt into the mounting hole from above. Tighten the bolt with a hexagonal screwdriver to secure the auxiliary support column to the stage. Do the same for the opposite side. (4) Insert a bolt into the threaded hole in the bottom of the auxiliary support column, tighten the bolt and secure the auxiliary support column to the microscope body. Do the same for the opposite side. * When the LWD condenser is attached, the stage support columns cannot be attached. You must attach the stage support columns before attaching the LWD condenser. 4 Attaching the stage support plates Attach the stage support plate onto the stage. And then, fix it with four bolts. Do the same for the opposite side. 43 Mounting hole for the auxiliary stage support column Fix the auxiliary support column to the stage. Stage support plate Fix the auxiliary support column to the microscope body. * Do the same for the opposite side.

46 Chapter 4 5 Assembly Attaching the LWD condenser (1) Rotate the condenser focus knob on the substage to lower the condenser holder to the lowermost position. And then, insert the LWD condenser into the condenser holder. Insert Condenser focus knob (2) Adjust the LWD condenser so that the aperture diaphragm lever comes to the front. Then, tighten the clamp screw on the left side. Clamp screw Aperture diaphragm lever 6 8 Attaching the chamber holder and the acrylic chamber (1) Place the chamber holder on the stage with the flat surface facing up, then attach the acrylic chamber on the chamber holder. (2) Adhere a cover glass to the bottom of the acrylic chamber with a silicone adhesive or so on to prevent water leaks. Chamber holder Acrylic chamber Attaching the sliding nosepiece or the single objective holder (1) Rotate the coarse focus knob to move the nosepiece arm to the center of the vertical stroke. (2) Insert the provided hexagonal screwdriver into the hole on the right side of the eyepiece mount part. And then, tighten the M4 hexagonal socket head set screw on the right side of the arm. (3) Insert the sliding nosepiece or the single objective holder into the dovetail on the nosepiece arm. (4) Push in the sliding nosepiece of the single objective holder as far as it will go and fix it with the M4 hexagonal socket head set screw. 44 M4 hexagonal socket head set screw

47 Chapter 4 9 Assembly Attaching the polarizer turret (1) Insert the polarizer turret into the receptacle step on the microscope base. (2) Tighten the clamp screw when the polarizer turret reaches the limit. Clamp screw 10 Attaching objectives For the sliding nosepiece Rotate the coarse focus knob to move the nosepiece arm to the upper limit. Move the objective vertical movement lever on the sliding nosepiece to the center. And then, screw in the nosepieces. For the sindle objective holder Rotate the coarse focus knob to move the nosepiece arm to the upper limit. And then, screw in the LWD 16x water immersion type objective. 11 Attaching the DIC slider For the sliding nosepiece Set the objective into the optical path. Lower the objective. You can see the DIC slider slot. Insert the DIC slider into the slot. Use the DIC slider that meets the specifications of the objective. DIC slider For the sindle objective holder Insert the DIC slider into the DIC slider slot at the upper part of the clamp screw for the objective. DIC slider 45

48 Chapter 4 Assembly 12 Attaching the DIC module Attach the DIC module into the turret of the LWD condenser. Loosen the hexagonal socket head screw at the upper part of the LWD condenser with the hexagonal screwdriver. And then, remove the cover. You can see an opening for the DIC module and the diagonal illumination diaphragm inside the cover. Attach the DIC module into the opening. Cover To attach the DIC module N1 Rotate the turret so that you can see the OBL label at the side of the turret. This is the position to insert the DIC module N1. Label To attach the DIC module N2 Rotate the turret so that you can see the O label at the side of the turret. This is the position to insert the DIC module N2. 13 Connecting the optical fiber light source (1) Insert the optical fiber into the optical fiber adapter on the back of the microscope. (2) Push in the optical fiber as far as it goes, then fix it with the M4 hexagonal socket head set screw. Optical fiber adapter M4 hexagonal socket head set screw 14 Fixing the microscope main body Insert four hexagonal socket head bolts into the screw holes on the vibration isolator through the holes of the base auxiliary legs at the base of the microscope. And tighten the bolts to fix the microscope. To use XY mover manufactured by Narishige Co., LTD Loosen M5 hexagonal socket head bolts on the both sides of the microscope base to remove the base auxiliary legs. Mount the microscope on the XY mover and align the screw holes for the base auxiliary legs and the screw holes on the XY mover. Then, tighten M5 hexagonal socket head bolts to fix the microscope. XY mover fixing leg 46

49 Chapter 4 Assembly 15 Attaching the FN-MS Magnet Scale (1) Rotate the coarse focus knob to raise the nosepiece arm to the upper limit. (2) Loosen four screws using a Phillips screwdriver to remove the back cover of the microscope body at the rear side of the arm. (3) Fix the Magnet Scale into the place with two M3 screws. Do not place the detector part of the Magnet Scale in a slanting direction. (4) Draw the cable for the Magnet Scale through the opening of the back cover. And then, attach the back cover in place. Connect the cable to the [SIG. IN] socket on the back of the display part of the Magnescale. Screw holes for the Magnet Scale 16 Connecting the power code WARNING Make sure to use the specified power cord. When the supply voltage is 100 V to 120 V, the power cord specification is as follows. UL Listed detachable cord set, 3 conductor grounding Type SVT, No.18 AWG, 3 m long maximum, rated at 125 VAC minimum. When the supply voltage is 220 V to 240 V, the power cord specification is as follows. Approved according to EU/EN standards, 3 conductor grounding Type HO5VV-F, 3 m long maximum, rated at 250 VAC minimum. Connect the lamp house and the power supply with the extension cable. Check that the power switch on the power supply is turned off (it is flipped to the "O" side). Insert the socket of the power cord into the AC inlet on the back of the power supply. Then, securely plug the power cord to the wall outlet. 47