AxioCam ERc 5s. Completely Independent. New Standard for Education and Routine Applications: The Microscope Camera with Stand-alone Functionality

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1 Microscopy from Carl Zeiss AxioCam ERc 5s Completely Independent New Standard for Education and Routine Applications: The Microscope Camera with Stand-alone Functionality

2 AxioCam ERc 5s from Carl Zeiss Documentation Made Easy Performance in the most compact form: with AxioCam ERc 5s, Carl Zeiss expands the proven AxioCam family with a real power pack that provides the perfect solution for documentation, observation, and stand-alone use. Documentation, observation, discussion: the choice is yours The 5 megapixel CMOS camera AxioCam ERc 5s is an affordable documentation system that offers speed, ease of operation, and straightforward technology. This camera can be used as a full-fledged microscope camera for education and routine requirements or as a video device for observation with a fast and high-quality live image. During stand-alone operation, images are stored immediately on SD card and can be transferred easily to a computer at any time. All operating buttons are located directly on the camera housing: enormously efficient when documenting many samples quickly. AxioCam ERc 5s can also be operated remotely, so users can keep an eye on observation or experiment during documentation. What makes this system particularly comfortable for the user is Stand-alone functionality: automatic white balance and snap button to save the images directly on SD cards. that AxioCam ERc 5s can be used entirely independent of the PC. AxioCam ERc 5s is the ideal solution for routine applications. AxioCam ERc 5s is the ideal camera for the different microscopes in laboratory practice. 2

3 Examples of various routine applications: wool fibre (EDF image), micrasterias radiata, bone marrow, aluminium-silicum-magnesium alloy Performance and high resolution for greater certainty With AxioCam ERc 5s, Carl Zeiss offers expert technology at an excellent price-performance ratio. This 5 megapixel color camera sets a new standard in its class. High resolution, very good software integration and high image quality maintains the standard. This means, for example, that even very delicate structures are clearly visible. A fast live image with a higher resolution than video cameras: AxioCam ERc 5s and its image quality mark a new performance level in this camera class. This is made possible by various video outputs (USB, AV or DVI), so that devices such as a PC, a projector or a monitor can be connected directly to the camera. Rapid acquisition with outstanding image quality is possible added certainty for reliable results in medical reporting or quality assurance. Impressively versatile AxioCam ERc 5s can be used with all microscope stands from Carl Zeiss without exception. The option of standalone operation ensures even more efficient laboratory processes. Various interfaces allow the direct connection to a PC, a projector or a computer monitor. Fully integrated with the system software, AxioCam ERc 5s can be used as a conventional microscope camera. In combination with the additional software modules HDR Imaging or Interactive Measurement, all these systems offer easy operation, greatly simplified processes and maximum reliability for your day to day work in materials analysis and biological applications. The software configuration tool offers the opportunity to customize the individual settings for the stand-alone operation to perfectly match the user s application. The advantage is an optimal result in every workflow. Stand-alone documentation that can be used anywhere AxioCam ERc 5s has the flexibility you need, whether you use it in the software control mode or even independently of the PC by capturing images directly on a SD card. White balance can be carried out on the camera itself at the push of a button. The camera can be connected directly to a PC, a monitor or a projector for joint observation, even during stand-alone operation. An optional stand-alone package with all the components necessary for independent operation is available, including a power supply for worldwide use, a remote control, an HDMI-DVI adapter, and a SD card. With the software configuration tool, the user can change individual settings for stand-alone use such as labeling, color, brightness, and contrast. AxioCam ERc 5s Configuration Tool 3

4 AxioCam ERc 5s: Data and Facts Sensor Micron MT9P031, 5 megapixels, CMOS Stand-alone package (optional for use without PC) CCD basic resolution 2560 x megapixels Power supply Via mini USB 2.0 Output: 5 V / 1 A Input: V, 50/60 Hz with country-specific adapters Pixel size 2.2 μm x 2.2 μm Remote control IR remote control Sensor size 5.7 mm x 4.28 mm equivalent 1/2.5 (diagonal 7.1 mm) Memory card 4 GB SDHC card with case Spectral sensitivity Approx. 400 nm-700 nm, IR filter Card reader SD, MMC Range of integration time 10 μs up to 2 s Adapter HDMI to DVI Live image Max. 13 fps at 800 x 600 pixels, subframe Order number Read-out mode Progressive Digitization 3 x 8 bits/pixel Interfaces 1x SD card slot; 1x mini USB 2.0, 1x AV (S-Video); 1x DVI (HDMI) Relative spectral sensitivity AxioCam ERc 5s Optical interface C-Mount Housing (WxDxH) 65 mm x 65 mm x 63 mm Power supply Via USB 2.0 Dual Color LED Power on and ready for capture (green); Recording (blinking green); Not ready (red); Error (blinking red) Integrated slot SD card slot for SD and SDHC cards Recording Button for image capture Set new white balance Button for new setup Ambient conditions (operation) +5 to +45 Celsius, max. 80 % relative humidity, no condensation, free air circulation required Order number Relative spectral sensitivity Wavelength in nm Carl Zeiss MicroImaging GmbH Jena, Germany BioSciences Göttingen Location Phone : Telefax : micro@zeiss.de 4 Information subject to change. Printed on environmentally friendly paper bleached without cholorine /e printed 08.10

5 Microscopy from Carl Zeiss Axio Lab.A1 Ergonomic. Easy-to-Use. Durable. Developed for bio-medical laboratories, for universities and training courses: Axio Lab.A1 is unmatched when it comes to performance and optics. Highly ergonomic, easy to operate and brilliant image quality for versatile medical applications. Particularly in laboratory use, reliable microscopic images are crucial. With Axio Lab.A1 you are guaranteed to work with a microscope that combines maximum performance and cost-effectiveness. The special plus for long-term microscope users: TUV-approved ergonomics. Axio Lab.A1 has been designed for daily laboratory applications. All applications in brightfi eld, darkfi eld, phase contrast or fl uorescence can be performed readily with the full range of ZEISS IC 2 S objectives. Key benefits at a glance TUV-approved ergonomic confi guration for maximized comfort and a stress-free working position Special features for laboratory use, such as convenient position of all main operating elements and the use of soft-touch materials Excellent optical quality for reliable results Long term durability through the use of high quality materials Numerous tubes, including multi-observation and accessories for a wide range of applications Transmitted light illumination alternatively HAL 35 W, LED daylight or LED warmlight High reliability for continuous microscopy work.

6 The First Laboratory Microscope With Approved Ergonomics A number of attractive details are designed to guarantee the ergonomic and reliable handling of Axio Lab.A1. TUV-approved ergonomics. Axio Lab.A1 places strong emphasis on ergonomic features, because continuous microscope use is often associated with discomfort, partic ularly in the neck. With Axio Lab.A1 users are able to examine slides with a favorable viewing position, keeping the neck and shoulder muscles relaxed. The height of the microscope is continuously adjustable by 50mm. In addition the tube can be swiveled from 8-33 continuously. IC²S optics, the Infi nity colour-corrected system, is available only from Carl Zeiss. The system guarantees brilliant, colour-corrected and high-contrast results, meeting all the demands placed on image quality. TUV*-approved ergonomics: The ergo-version of Axio Lab.A1. Guaranteed durability. The more a microscope is used, the more worthwhile it is to invest in quality. Axio Lab.A1 guarantees long lasting value and a long operating life. The new multi-discussion system from Carl Zeiss is also compatible for Axio Lab.A1. It is possible to achieve identical image orientations for all co-observers. All co-observers see the same image as the main observer. And a luminous pointer facilitates the identifi cation of specimen details for discussion. Skin-friendly materials enhance comfort. Many parts of the stand are coated with soft-touch material: These materials offer superior properties in comparison to metal surfaces: less cold and higher friction. Comfortable and convenient: Working with supported arm and all main elements within reach of one hand. Modular illumination. Convenient and easy-to-change bulbs. The use of the novel warmlight LED in particular offers the advantages of a long lifetime, energy saving and halogen-like colour impressions in stained slides. * TUV Rheinland is a world-renowned independent organization which certifies product safety and ergonomy. Integrated tool storage and cable rest in the stand back fl ap. Tools, e. g. for centring of phase rings are always at hand and cannot get lost. In addition, the tilted fl ap can be used as cable rest. Everything at hand: Tool bar on the back of the scope. 2

7 Explore the Possibilities Axio Lab.A1 Wide Range of Applications With the help of Axio Lab.A1 diverse diagnostic applications can be carried out easily and effi ciently. Axio Lab.A1 is designed for many uses. Microbiology, cytology, hematology and pathology labs as well as those in school and colleges will benefi t particularly. Fluorescence microscopy in the lab Axio Lab.A1 ensures easy-to-use LED-fl uorescence with 2 LED positions and the well known standard push-andclick-modules from Carl Zeiss. In comparison to standard HBO illumination the LED fl uorescence is much safer, more energy effi cient, quicker and easier to use. Additional advantages: No warm-up and cool-down times and no need to change or adjust lamps. The fl uorescent marker FITC is primarily used for antigenantibody reactions in the fi eld of immunology. FITC binds to the antibody molecules and emits an intense green fl uorescence upon excitation with the 470 nm LED. Brightfield and darkfield microscopy In the fi eld of hematology, the usual diagnostic approach to blood disorders is blood counting and blood fi lm examination. During blood fi lm examination in brightfi eld microscopy, the individual types of white blood cells are counted. Axio Lab.A1 makes this task easier, because the main operating elements of the microscope, such as the stage drive, fi ne focus drive and light intensity are all reach of one hand. This frees up the other hand. Darkfi eld blood analysis is very useful for the early detection of serious health conditions, because fi ne and unstained structures can often not be seen in front of a bright background. This situation changes if the structures are illuminated from the side and viewed in front of the darkest possible background. The structures then really seem to light up. Darkfi eld is a useful detection method for minute details, not easily detectable brightfi eld. It is produced by indirect sample illumination resulting in bright structures on a dark image background. Main fi elds of applications are hematology and dermatology. Axio Lab.A1 presents a fi ve position Abbe turret condenser with darkfi eld and Phase contrast 1,2,3. Polarization contrast is used to detect birefringent structures, such as crystals or fi bres. In medical microscopy it helps to detect gout or asbestos. Hematology: Plasmodium malariae, daisy-head stage in brightfield. Specimen: Andrea Michelsen, Ortenau Klinikum Lahr-Ettenheim, Germany. Mouse kidney after staining with FITC. Hematology: Blood smear in darkfield. Gout inspection: Uric acid crystals under polarization contrast. 3

8 Axio Lab.A1 Technical Data Contrasting techniques Illumination Objectives Eyepieces Stand Ergomomics Co-observation Brightfi eld, darkfi eld, phase contrast, simple polarization contrast, fl uorescence contrast Integrated 35 W halogen lamp, 3 W LED (daylight, warmlight) A-Plan, N-Achroplan, EC-Plan-Neofl uar and all other IC 2 S objectives Eyepieces 10 x for fi eld of view of 20 mm or 22 mm 5-position nosepiece, stage drive right TUV-approved ergonomics with height-adjustable and tilting tube, with ergonomic stage and stage drive, with a number of soft-touch materials to increase working comfort One main-observer with tubes for up to 2 co-observers seated on the left side of the main observer All optics in Axio Lab.A1 are anti-fungus treated Norms and standards met: CE, CSA(UL), IvD, DIN EN (IEC ), ISO 9001, ISO Available optional accessories: mechanical stages with drives right or left or right in fi xed position (ergodrive), several types of specimen holders, several condensers, several binocular tubes, phototubes and ergotubes with different adjustment possibilities in respect to height and tilt, multi-observation equipement, several interference and neutral density fi lters, several illumination bulbs as HAL or LED, several polarizer and analyzer moduls, several reflector modules, several FL-LED modules, several camera adapters, several microscope cameras and AxioVison microscope software. Carl Zeiss MicroImaging GmbH Jena, Germany BioSciences Göttingen Location Phone : Telefax : micro@zeiss.de Information subject to change. Printed on environmentally friendly paper bleached without chlorine /e printed 04.10

9 Microscopy from Carl Zeiss Axio Scope More functional. More economical. The adaptable all-round stand for your routine and research applications

10 Your Axio Scope More functional in every component. More economical in every configuration. Axio Scope is your microscope down to the last detail. Modularity has always been a prerequisite for customized microscope configurations. However, modularity has never before been implemented so consistently. The new all-round stand from Carl Zeiss is your microscope in every respect. With its unusual flexibility, it can be used universally and is yet individually tailored to your applications. More economical than ever because you only buy what you need at the moment. And because you have the option of extending your microscope to meet your growing demands. You will be equally impressed by the cost benefits and the performance. Axio Scope the latest generation upright routine microscope. 2

11 Contents Flexibility 6-7 Transmitted-light Techniques 8-9 Fluorescence Fluorescence LED Objectives 14 Imaging System 15 Specimen Space Operating Comfort Axio Scope: System Overviews The New Functionality: All the Advantages 25 3

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14 Flexibility Twenty-three Stand Versions. Innumerable Interfaces. An Outstanding Solution for Your Applications and Budget. An unusual number of stand versions and interfaces results in unusual flexibility. This means that you can configure your Axio Scope whatever way is useful for you. Functionally and economically. New modularity Axio Scope is as individual as the applications that you use it for. You can customize your stand from five upper parts, three lower parts and two vario columns. For simple or more complex tasks. For transmitted-light, reflectedlight or fluorescence. What is important is that you no longer invest in functions that you don t even use a tangible advantage for your budget. Economical upgrading Simpler and less costly the modular interface concept of Axio Scope makes future upgrading quite simple. This is economically attractive as you can install many of the components yourself without waiting for expensive service support. Versatile range of uses Axio Scope provides a range of uses which are as versatile as the tasks in institutes and laboratories. They range from simple routine applications to more complex research projects. From anatomy to cytology. From transmitted-light applications to multi-fluorescence labelling. From the thinnest histological section to a specimen 380 mm thick. The upper parts I. For pure transmitted-light applications Equipment: objective turret with 6x BF II. For standard fluorescence applications Equipment: objective turret with 3x DIC/3x BF, standard interface for HBO 50, HBO 100, HXP 120, Colibri etc. III. For LED fluorescence applications Equipment: objective turret with 3x DIC/3x BF, integrated illumination that can accept 4 different LED modules, synchronized switching with the reflector turret, long operating life, highly economical IV. For reflected-light and fluorescence applications Equipment: objective turret 6x BF/DF, standard interface for HAL 100 or HBO, DF insert in the objective turret, switchable diffuser V. For reflected-light and fluorescence applications Equipment: objective turret 6x DIC/DF, standard interface for HAL 100 or HBO, DF insert in the objective turret, switchable diffuser, accessible aperture and field diaphragm levels, slot for polarizer slider BF = Brightfield, DF = Darkfield, DIC = Differential Interference Contrast I II III IV V 6

15 The new variety of interfaces shown here, using the example of upper part III and lower part B. They vary according to the upper and lower parts. 1 Reflector space 2 Tube 3 Eyepiece 4 Camera adapter 5 Camera 6 HBO adjustment aid 7 Reflected-light filter slider 8 Achromatic illumination adapter 9 Reflected-light source 10 Upper part/lower part 11 Spacer 12 Lower part/upper part 13 Transmitted-light filter slider 14 Transmitted-light filter wheel 15 Condenser carrier 16 Condenser 17 Modulator disc 18 Stage 19 Stage carrier 20 Objective The lower parts A. For many routine applications Illumination: LED (Fixed-Koehler), screwed in directly beneath the condenser carrier B. All standard applications Illumination: 50 W reflector light, koehlerable beam path with field diaphragm and aperture diaphragm, filter slider and 6-position filter wheel C. For demanding transmitted-light applications with high-intensity illumination Illumination: 100 W halogen, koehlerable beam path with field diaphragm and aperture diaphragm, filter slider and 6-position filter wheel The stand columns For examination of thick specimens D. Vario column 380 mm for reflected-light and fluorescence applications E. Vario column 560 mm for reflected-light and fluorescence applications A B C D E 7

16 Transmitted-light Techniques LED or Halogen, Phase Contrast or DIC. Never Before has a Microscope of This Class Offered so Many Possibilities. 1 Whether unstained cells, histologically dyed sections or bone samples: transmittedlight techniques continue to be the standard for many examinations in the clinical area. The interfaces developed for the Axio Scope are unique. This brings completely new flexibility into routine microscopy. All contrasting techniques Surprising in this microscope class: the sheer variety of contrasting techniques that you can use for your applications. Axio Scope offers you simply everything. The classical methods of brightfield, darkfield, phase contrast but also Differential Interference Contrast, DIC for short. You can also use the economical PlasDIC alternative from Carl Zeiss. Which means that this inexpensive interference contrasting technique is available for the first time with an upright microscope. Versatile options: the LED components (1), the Abbe condenser with/without modulator disc (2), and the modulator disc with exchangeable modules for darkfield, phase contrast or PlasDIC techniques (3). Individual configuration: the transmitted-light beam path The transmitted-light beam path of Axio Scope provides impressive image contrast and homogeneous illumination. The great flexibility in configuring offered by Axio Scope is new and unique. At the front you have a 6-position filter wheel, which comes equipped with the integrated shutter. Individual grey and colour filters can easily be added. At the back you can also use an additional filter slider, which can also be configurated flexibly so that nearly any combination of filters is possible. Moreover, if you decide on transmitted-light LED illumination, attenuating filters are unnecessary. The economical LED illumination can be attenuated as desired without any change in colour temperature a genuine advantage compared to halogen light. It s easy to change to darkfield, phase contrast and PlasDIC: the interfaces in the Abbe condenser and modulator disc provide a unique range of contrasting techniques. Simply by inserting the modules

17 The 6x filter wheel comes equipped with an integrated shutter. Grey and colour filters can be mounted individually easily and conveniently since the filters are clicked readily into the corresponding holders when the cover is open. LED or halogen? Standard illumination You can choose among three subunits optimized for the different geometries of the light sources. 1. Transmittedlight LED: durable, economical and adjustment-free, this illumination provides sufficient intensity for stained histological preparations and other typical routine clinical tasks W reflector illumination: this offers more than enough light for all standard procedures such as brightfield and phase contrast W halogen light, ideal for thick specimens and procedures that require a lot of light, such as DIC at high magnifications. Flexible for interference contrast: the Abbe condenser The possibility of equipping Axio Scope with anything from a very inexpensive bright field condenser to a highperformance interference contrasting technique is new and unique in this class. This is made possible by two unusual interfaces: you simply equip your condenser with the modulator disc for darkfield and phase contrast when you need these methods. The modulator disc itself has exchangeable modules. This allows you to use even PlasDIC, the inexpensive interference contrast from Carl Zeiss, at any time, simply by exchanging the modules. ISCP: the Infinity Space Camera Port For all documentation tasks in transmitted-light applications and only with Axio Scope: the Infinity Space Camera Port, ISCP for short. This innovative feature offers you an additional option for using the interface in the infinity space (see also infinity space interface on pages 10/11). Instead of using this for reflected-light reflectors, you can use it for your applications in transmitted-light. That way, you gain a fully functioning camera port, independent of the choice of tube that can be added at any time. ISCP the Infinity Space Camera Port - is simply inserted in the interface in the infinity space. For transmitted-light, it is a fully functioning camera port, that can be added any time. 9

18 Fluorescence Configured Today. Adapted Tomorrow. The New Fluorescence Grows With Your Demands. FluoresScience is the name of the initiative reflecting Carl Zeiss focus on the development of excellent fluorescence techniques. The latest developments of this initiative reflect the growing importance of fluorescence as a routine method. With outstanding quality, innovative solutions and its ability to be configured to meet requirements down to the smallest detail, Axio Scope sets quite new signals in its class. Can be retrofitted to meet your needs: the reflected-light beam path Axio Scope is excellently equipped for fluorescence because the reflected-light beam path was completely recalculated to provide outstanding contrast and excellent minimizing of scattered light. On the other hand, you can benefit from the great flexibility of the modular system here. The adjustment aid for the HBO can be added you can simply insert it yourself. The same applies to the achromatic system, which guarantees you even better results in Multichannel Fluorescence applications. Interface for your reflector inserts: infinity space Unique in this class: the interface in the infinity space. With Axio Scope you can select the reflector turret or slider required for your applications. Flexible, simple and quickly added. You have a choice of a 2x slider or a reflector turret with 4 or 6 positions. In addition, Axio Scope offers another feature here as the interface can also be used as a camera port for transmitted-light applications (see Infinity Space Camera Port, pages 8/9). Exchangeable: the standard lens system and the achromatic system for Multichannel Fluorescence applications. The interface in the infinity space allows diverse uses: the 6x and 4x reflector turret, the Infinity Space Camera Port (ISCP) and the 2x reflector slider. An option that can be added simply: the adjusting aid for setting the HBO burner. 10

19 Versatile and innovative: the light sources The illumination options of Axio Scope are versatile and simple for the customer to operate. A completely new lamp housing was developed for the HBO 50, whose burner can be removed with the drawer for a particularly simple and convenient change of burner. The HBO 100 comes as a standard or in a self-adjusting version. In addition to HBO illuminators the HXP 120 provides you with a durable, adjustment-free metal halide light source with integrated fast shutter, which can be controlled simply by a hand or foot switch or from AxioVision. Simpler than ever: changing the burner in the newly developed HBO 50 lamp housing. Inserting the reflector slider, reflector turret or ISCP in the infinity space on the vario stand: just one simple movement of your hand. 11

20 Fluorescence LED Completely New. Completely Integrated. With This Innovative Light Source Axio Scope Sheds New Light on Routine Work. A technological development, available for the first time with Axio Scope: the integrated LED illumination for routine fluorescence applications. Now you can have these extremely durable and economical light sources in both transmitted-light and reflected-light. The new LED The advantages of LED illumination speak for themselves: extremely long operating life, inexpensive, easy to handle, and completely adjustment-free. And without expensive changes of burner for a very long time instead of operating constantly, the LEDs turn on only when they are used in the beam path. Now Carl Zeiss offers a new integrated LED solution specially designed for routine applications in fluorescence. The freely mountable LED turret allows you to you use up to 4 LED modules. Synchronized with the reflector turret*, the correct LED is switched on automatically when a new reflector module is swung in. The intensity can be regulated individually for any colour. Your settings are preserved even when you switch back and forth an important plus because that way each of your different fluorescence channels is optimally illuminated at any time. Without a tool, easy, and safe: mounting the LED modules on the LED turret. When the cover is opened, a safety switch ensures that the LED is switched off immediately. * Because of the coupling of reflector turret and LED module turret, the reflector turret is not exchangeable with this upper part. 12

21 Full Flexibility The flexible modular concept of Axio Scope leaves you much room for configuring your stand to meet your needs. The upper part with the integrated LED fluorescence (see page 6/7, upper part III) can be combined with all lower parts. If you wish, you simply assemble your complete LED stand with integrated transmitted-light and reflected-light LED. Prepared for Colibri Axio Scope is prepared for Colibri, the high-end LED fluorescence light source from Carl Zeiss. Thus you have a choice to use a light source, which is fully integrated in the AxioVision software. And so you are able to take automatic Multichannel Fluorescence images on a purely manual stand! Easily accessible: on/off switching and intensity control of the LED modules. Colibri for setting up to four wavelengths simultaneously. 13

22 Objectives New for Routine Work. Tried and Tested for the Highest Demands. Objectives from Carl Zeiss: the Economical Solution for Every Requirement. Carl Zeiss stands for more than 150 years for microscope lenses that are practically unrivalled. And this in all performance classes. Naturally highest demands on optical performance were also placed on Axio Scope. Each class is a class of its own the objectives N-ACHROPLAN objective series: The new inexpensive all-round talent: the N-ACHROPLAN objective series was developed specially for diverse routine applications. Perfect for routine work: the new N-ACHROPLAN objectives Newly developed and perfect for many of your routine applications: the N-ACHROPLAN series. A very inexpensive all-round objective with good chromatic correction and planarity. Moreover, the entire range of objectives from Carl Zeiss are at your disposal. The decision is up to you; from a low magnification overview to high-resolution imaging of details. A-Plan the A class: Good and inexpensive entry-level objectives for diverse applications; good optical quality for transmitted-light applications N-ACHROPLAN the all-rounders: Inexpensive, versatile and new for many routine applications in transmitted-light or reflected-light EC Plan-NEOFLUAR the universal talents: Perfect when flexibility and a variety of techniques are required. Based on the IC 2 S optics, they are characterized by high-contrast imaging, excellent homogeneity, high resolution, and high transmission. FLUAR the photon collectors: High numerical apertures, good contrast, and outstanding transmission provide you with enormous optical performance. Ideal for rendering the weakest fluorescence visible Plan-APOCHROMAT the high-performance series: Best colour correction and highest numerical apertures for brilliant images in all techniques. Convincing: the outstanding point spread function and spherical correction Unusual in this performance class: the interfaces for DIC. Depending on the upper part, an objective turret with 0, 3 or 6 DIC slide holders is available with Axio Scope. Six objective positions offer you plenty of scope for mounting them to suit your application. 14

23 Imaging system Flexible for the Leading Software and Every Camera Type. The Simple Step from Stand-alone Stand to Documentation System. Your Axio Scope Imaging System is more than the sum of microscope, software and camera: a complete solution from a single source. Integrated fully into the Carl Zeiss system approach. With security of one hundred percent compatibility also for future developments. Very convenient: AxioVision microscope software Easy to operate and functional with AxioVision you extend your Axio Scope to include digital documentation. The modular microscope software from Carl Zeiss offers you all the important functions in the basic version, from recording and processing to analyzing and archiving your images. AxioVision is one of the leading systems on the market, not least because this software is surprisingly simple to operate despite its wealth of functions. For instance, it is possible to adapt the operating interfaces and functions yourself ideal for simplifying your routine applications through clear processes, thus making them more efficient. Moreover, you get the AxioVision LE software version free of charge. A good solution that opens up many options in digital photography. Great in all performance classes: the cameras From AxioCam ICc1 or AxioCam ICc3, the smallest and most inexpensive digital colour cameras, to the highspeed AxioCam HS: Axio Scope provides a connection for every type of camera. And Carl Zeiss provides the complete range of modern microscope cameras. Each of them carries the Carl Zeiss seal of quality. Axio Scope is also compatible with digital consumer cameras, e.g. from Canon. The free AxioVision LE software provides a variety of high-quality functions for capturing, processing and analyzing images. 15

24 Specimen space Space for Every Specimen. Space for Every Experiment. The Flexibility of the Specimen Space: a Special Feature That You Will Welcome. The specimen space can be extended practically as much as you want. A strong plus point, because there are practically no limits with regard to size of specimen. Depending on the configuration the specimen space can be varied continuously: for specimen thicknesses from 0 to 110 mm. Flexible option for further extension of the specimen space in z direction: spacer in 30 mm and 60 mm versions. Where there are no limits to height The great scalability of the specimen space is an important basis for the range of uses of Axio Scope. In addition to the z travel range, it can be influenced in several places. 1. You can simply lower the stage carrier at the dovetail. 2. The condenser carrier can also be removed easily, which is practical when you want to lower the stage over the travel range. 3. You can gain space at the interface between the upper and lower parts by using a spacer. This comes in 30 or 60 mm versions. Depending on the configuration, the specimen space can be extended smoothly by up to 110 mm. The modular interface concept allows easy handling, e.g. when using the spacer (30 mm or 60 mm) to enlarge the specimen space. 16

25 When even more space is needed If you require even more space for unusually large specimens, e.g. in intravital microscopy, Axio Scope offers two vario stands for your reflected-light and fluorescence applications. Depending on your requirements, you can couple any upper parts to them. A perfect fit for your applications. There is virtually no limit to the size of the specimen space: specimens up to 380 mm in size, such as this tree trunk, can be examined with the vario stand columns. The specimen space is continuously adjustable for specimen thicknesses from 0 to 110 mm, simply by adjusting the height of the stage carrier. 17

26 Operating Comfort Convenient to Handle. Efficient in the Workflow. Operating Comfort and Ergonomics Fulfill Almost all Your Needs. Developed for 24-hour use, Axio Scope will convince you by its quality and stability. The many intelligent details making your routine work in the laboratory a great deal simpler and more pleasant to work with. Well-conceived operating elements Simply intelligent the design of Axio Scope makes operating the stand in routine work as comfortable and safe as possible for you. The intensity setting for the transmittedlight illumination is arranged ergonomically around the focus drive. Positioned on both sides close to the focus drive, it is extremely comfortable to operate. You can now adjust the illumination settings without taking your hand off the drive, with your right or left hand, just as you prefer. Well-conceived operating comfort can be found in many elements in Axio Scope: the stage drive of the new ergo-stage, which can be moved in the y direction and fixed in place; the focus drive; the 6x filter wheel with integrated shutter; and the right/left hand intensity adjustment of the transmitted-light illumination. New stages Axio Scope offers a wide range of different stage versions. Two stage innovations are outstanding. 1. The ergostage: it enables you to move the stage drive 90 mm along the y axis into the position that is most comfortable for you. It can be fixed in that position and easily operated, without any need for you to change the position of your hand. 2. The new stage with a light special coating: this innovation is ideal for routine applications with a high throughput of specimens. In other words, when many slides are moved on the stage which used to be the cause of early wear and tear on the stage surface. The extremely hard coating of the new stage shows no scratches, even in a hardness test. 18

27 Smart slide guide An intelligent innovation that experienced clinicians in particular will welcome: the slide guide of Axio Scope alternatively allows you to guide the specimen by hand. Designed as a flat plate, it is ideal for pushing your specimen slides back and forth so that you can quickly obtain an overview. If you want to view certain areas at higher magnification, simple let go and use the sensitive stage drive. Ergonomic tubes Designed for long laboratory work days, Axio Scope places a special emphasis on ergonomics. The viewing angles of the ergotubes are based on modern studies and are designed for fatigue-free and healthy posture. Two different models are available. The height-adjustable ergotubes with a travel range of 44 mm or the ergo-phototube with upright correct-side image, which can be adjusted through 50 mm in height and depth at a fixed viewing angle of 15 so as to be as comfortable as possible for your height. The eyepiece setting can also be varied with both tubes, enabling you to gain an additional 50 mm of height. Conversion without tools More comfortable, faster, and simply practical the modular interface concept of Axio Scope is designed so that you can simply remove many components, such as the stage carrier or condenser, without any tools. This simplifies the cleaning process, for example. Robust construction Whether pathology, cytology or histology: in clinical routine work, where specimens are often examined in a 24-hour throughput, the material and design of the microscope are important. And here Axio Scope is a true Carl Zeiss microscope: robust stand design and superior mechanical quality. The extremely durable special coating for toughest continuous use. With the smart slide guide the specimen can be moved around efficiently by hand. Alternatively, the more precise x/y stage control can be used at high magnifivation. 19

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32 System Overview 24

33 The New Functionality All the Advantages 23 stand versions a perfect solution You configure your Axio Scope from five upper parts, three lower parts and two vario columns. Made to measure for simple routine applications, for demanding research tasks, for every budget. Countless interfaces An unusual number of interfaces allows exact adaptation to requirements and budget. Economical upgrading Many components can be added on by the customer as needed easily and without additional service costs. PlasDIC and DIC now in an upright microscope In response to many customers a choice of two sophisticated contrasting techniques is available in an upright microscope for the first time: Differential Interference Contrast, DIC, and PlasDIC, the inexpensive relief contrast. LED in transmitted-light LED in fluorescence Axio Scope offers integrated solutions with these extremely long-lasting and economical light sources, not only in transmitted-light but also in reflected-light thus taking into account the growing importance of fluorescence as a routine method. HBO 50 innovation Newly developed for Axio Scope, the burner can now be removed with the drawer for a particularly easy and comfortable change of burner. A variety of reflector inserts Through its customer interface in the infinity space, Axio Scope allows users to select the reflector insert most suitable for the respective application. There is a choice of a 2x slider, a 4x reflector turret and a 6x reflector turret. ISCP innovative option for transmitted-light applications The interface in the infinity space can be used not only in reflected-light (see above, use of reflectors) but also in transmitted-light: as a fully functioning camera port. Independent of the tube, it can be added on any time. N-ACHROPLAN objectives perfect for routine work The inexpensive, completely new developed objective series has been calculated for a field of view of 23 mm and is versatile for various applications. Flexibility of the specimen space Axio Scope offers unusually large scalability of the specimen space for the most varied applications. Depending on the configuration, continuously adjustable for specimen thicknesses from 0 to 110 mm. Convenient intensity adjustment The intensity adjustment of the transmitted-light illumination is arranged on both sides close to the focus drive and extremely comfortable to operate with the right or left hand. New development: the ergo-stage Newly developed in order to make hand positions individually adaptable and operation even more comfortable. The stage drive of the new ergo-stage can be pushed 90 mm along the y axis and fixed in the position that is most comfortable. Stage for high specimen throughput With an extremely hard special coating, this new stage remains completely free from scratches even in a hardness test ideal when a lot of specimen slides have to be moved on the stage. Smart specimen guide Axio Scope specimen guide allows the slide to be moved alternatively by hand an innovation that experienced pathologists in particular will welcome. Conversion without tools Many of the components such as the stage carrier can simply be unscrewed without tools, e.g. for cleaning purposes. 25

34 Carl Zeiss MicroImaging GmbH Jena, Germany BioSciences Göttingen Location Phone: Telefax: Information subject to change. Printed on environmentally friendly paper bleached without cholorine /e printed 04.08

35 Microscopy from Carl Zeiss Axiovert 40 Number One In Its Class The routine inverted microscope for cell biology We make it visible.

36 Quality and Performance. Demands become a reality. Confronted with the increasing demands of your laboratory s routine applications, you can't afford to compromise on quality. Nor can you afford to settle for less than the best in the optical tools you use. This is where an innovative inverted microscope, the Axiovert 40, steps in, setting new standards of quality. Its superior optical performance and exceptionally wide range of functionalities place it at the top of its class. In terms of flexibility, operating comfort, stand stability and component quality, it is obvious that the Axiovert 40 is another outstanding Carl Zeiss microscope. A microscope that meets the demands of professionals who: work primarily with living cells place exceptional demands on performance and functionality want a more cost-effective and efficient microscope in the lab appreciate flexible and easy upgrading options expect an investment in a microscope to be a safe investment. What does this mean for you? Axiovert 40 is the perfect microscope to deliver the added performance and quality that you need to make your demands become reality and, above all, at a price that makes high quality an affordable standard. Axiovert 40 the best inverted microscope in its category. 2 More efficient ++ More flexible ++ More time for observation

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38 The Optical System. Simply the Best. Axiovert 40 inverted microscope technology that offers you much more than you have ever expected from a microscope in this category. You will be impressed by the outstanding quality, and functionality, the wide range of excellent objectives, and the sophisticated fluorescence. And you will also appreciate the brilliance of the well-known transmitted light contrasting techniques right up to the innovative and unique PlasDIC, which is cost efficient and provides the best possible contrast. The Optics ICS Infinity Color-Corrected System provides the basis for the wide range of Carl Zeiss objectives, ensuring outstanding performance in all contrasting techniques. With the Axiovert 40, you can choose from a broad spectrum of objectives: CP-Achromat and LD A-Plan from the attractive Long Distance series right up to the high-performance objectives from the Plan-Neofluar series. Another benefit of Carl Zeiss optics: more light for more contrast. A powerful 35 watt lamp improves the brightness in transmitted light in all contrasting techniques. With a lifetime of over 800 hours, this halogen lamp is long lasting, too. Fluorescence For all applications based on GFP (Green Fluorescent Proteins) and other live cell fluorophors, you can attain exceptionally high fluorescence standards using the Axiovert 40 CFL. A significant benefit: even more stray light can be eliminated thanks to the newly developed 3-position reflector mount. As a result, you enjoy even better contrast, and for a quick exchange of filter sets: just Push&Click. Contrasting Techniques for Thin Specimens Axiovert 40 offers two effective techniques for applications with thin specimens: brightfield for the brilliant contrasting of naturally colored specimens such as plant protoplasts, and phase contrast for colorless thin cells or cell branches. The phase diaphragm is centerable, and can be used for a wide variety of purposes. Simple post-centering enables adaptation to the meniscus with small volumes of culture media, e.g. with multi-wells. 1. HeLa cells with different fluorophors. R. Brack-Werner, Institut für Molekulare Virologie, GSF Neuherberg T3 cells, phase contrast. B. Busse, In Vitro Systems & Services GmbH, Göttingen.

39 Axiovert PlasDIC the patented relief contrast technique for routine lab applications. Its outstanding benefit: the specimen area is located outside the polarization-sensitive zone. PlasDIC is the first differential interference technique based on polarization optics that permits the use of plastic vessels. The PlasDIC principle Coherence diaphragm Area not sensitive to polarization The Innovation: PlasDIC In addition to VAREL (variable relief contrast), Carl Zeiss is now offering an innovative and unique technique: PlasDIC, the first differential contrasting technique tailored to routine laboratory applications. The result: impressive relief effect and needle-sharp contrast across the whole specimen area, even on thick areas, that were a problem up to now. A significant gain in information! The contrast setting is variable and can be optimally adapted to your specimen. In addition, as with Nomarski DIC, different levels can be focused. PlasDIC is the technique of choice for thick structures such as cell aggregates and cell clusters (e.g. apoptosis stages). With its unique design, PlasDIC can be used with cells grown in standard plastic vessels, where cells often thrive. Conclusion: PlasDIC is an ingenious, innovative and costeffective technique setting new and exciting standards in routine inverted microscopy. Plastic dish + specimen Objective Polarizer + prism Analyzer The contrasting techniques in transmitted light Thin specimens and specimen areas: Brightfield Phase contrast Area sensitive to polarization Thick specimens and specimen areas: VAREL (variable relief contrast) PlasDIC (innovative differential interference contrast) 3. With the help of PlasDIC, you can identify different levels and details of the specimen. HEK cells after transfection. C. Lücking, Institut für Neurogenetik, Klinikum Großhadern HEK cells. C. Lücking, Institut für Neurogenetik, Klinikum Großhadern. Know-how from Carl Zeiss: the right PlasDIC slider for every objective. 5

40 Form and Function. The Perfect Relationship. High throughput of cell and tissue specimens laboratory applications require precision and reliability in workflows that are not often similar or identical but must also be carried out under great time and budget pressures. With its numerous features, Axiovert 40 is ideal for these situations. The size and weight of its stand is tailored to meet the realities of day-to-day lab work, the stability is outstanding and the quality, first-class. In other words: Axiovert 40 provides a wealth of impressive features that make working in the lab easier, more efficient and more cost-effective. Operating Ease The Axiovert 40 is easy to operate. A large glass stage ensures free view of the nosepiece and makes it possible to identify the specimen quickly. The large surface of all our stages makes it safe to handle a variety of culture vessels. Of particular importance, when different culture vessels are used, is the sliding condenser. It enables you to adapt the microscope to large vessels such as roller bottles quickly and easily. Three contrasting techniques in one objective e.g. brightfield, phase contrast and PlasDIC guarantee fast and precise specimen analysis. It's also fast and easy to change magnifications. With Axiovert 40 you can can easily switch your viewing angle from the cultivation vessel (macro positioning) to the tube and back again. Operating Comfort Concentrated work at the microscope necessitates a relaxed and upright posture a requirement that the Axiovert 40 easily fulfills. The Siedentopf tube offers two viewing heights, so that observation is always comfortable. Yet another ergonomical benefit is provided by the intelligent design. You can easily switch between viewing the specimen 1. Practical: When using large cultivation vessels, simply push the condenser back Exchanging the reflector modules for fluorescence: just Push&Click.

41 Axiovert No unnecessary manipulation: With PlasDIC it is not necessary to adjust the condenser when changing the objective. 1 diaphragm 4 magnifications 10x 20x on the stage for macro positioning, and viewing of the specimen through the microscope an essential prerequisite for successful routine applications. In addition, the camera does not block your view since it is mounted to Axiovert 40 via a front port. 32x 40x PlasDIC: All magnifications without changing the diaphragm. Stability Durable, sturdy, expertly made and simple to operate: it's easy to see that Axiovert 40 has been designed for your benefit. The successful pyramid shape with its low center of gravity provides rocksolid stability and vibration-free work that is unique in this category. This, of course, is critical for documentation and a must for micromanipulation. 1 objective 3 contrasting techniques Brightfield Ph Phase contrast PlasDIC A-Plan 10x LD A-Plan 20x LD A-Plan 32x LD A-Plan 40x A-Plan 10x Easier and faster operation, more flexibility: With one objective you can use three contrasting techniques in transmitted light. Brightfield Ph Var Phase contrast VAREL LD A-Plan 20x LD A-Plan 32x LD A-Plan 40x HEK cells, PlasDIC. C. Lücking, Institut für Neurogenetik, Klinikum Großhadern. 4. Tobacco protoplasts in brightfield. H.-U. Koop, Institut für Botanik and Zellbiologie, LMU München. 7

42 Flexible for Upgrades. Prepared for the Future. In routine laboratory applications, a lot is expected of your microscope. Demands are constantly changing. Consequently, innovative microscope technology must be versatile, flexible and upgradable. Just like Axiovert 40. Completely integrated within the Carl Zeiss microscope systems, this inverted microscope allows for easy upgrade options. This means that you only invest in the equipment that you need today while having the freedom to upgrade at any time for future applications right up to a digital imaging system. Technical Upgrades Highly flexible and exceptionally versatile: the Axiovert 40 combines outstanding performance with high-end options such as: variable components, objective options, sophisticated fluorescence and the innovative PlasDiC. Three different condensers, specimen stage, specimen stage glass, object guide, a wide range of mounting frames for various culture vessels, and temperaturecontrolled components an impressive proof that Axiovert 40 is based on flexibility and versatility. Mounting frames Axiovert 40 C Axiovert 40CFL Camera mount Brightfield Phase contrast VAREL PlasDIC Fluorescence basic equipment optional Numerous options for a wide range of applications with a choice of mounting frames, ensures that many kinds of cultivation vessels can be held securely. Upgrades 8 Axiovert 40 CFL, brightfield, phase contrast, PlasDIC, fluorescence Axiovert 40 CFL, brightfield, phase contrast, fluorescence Axiovert 40 CFL, brightfield, phase contrast, PlasDIC Axiovert 40 CFL, brightfield, phase contrast Axiovert 40 C, brightfield, phase contrast, VAREL Axiovert 40 C, brightfield, phase, Axiovert 40 C, brightfield

43 Axiovert Cameras Axiovert 40 can accept all types of cameras: SLR, video, digital compact consumer cameras and digital microscope cameras. If your requirements are demanding, you have the powerful range of digital cameras from the AxioCam family at your disposal. Cameras made by Carl Zeiss: superior image and color quality for a wide array of applications. Documentation yes or no? With Axiovert 40 it s easy to document your results since both versions of the stand come equipped with a front port making Axiovert 40 unique in its category. And you are free to decide on your camera of choice: simply select the suitable adapter. Image Processing Cost-effectiveness is part of the package: cameras from the AxioCam MR series come equipped with basic software. It provides you with everything you need to document your images fast and efficiently via PC from camera control and optimal depiction of dynamic range to data storage in selectable standard formats. If you want more than the basic software: AxioVision provides you with high-end software for sophisticated image processing, image analysis and image archiving. With its wealth of customized modules, this power package is your gateway to a world of countless possibilities. Glass stage Specimen stage Object guide Transparent for optimum view of the objective, sturdy or sophisticated: stage solutions for Axiovert 40. 9

44 10 The System Behind Axiovert 40.

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46 Axiovert 40 12

47 A Convincing Performance. All the Data, All the Facts. Stands Axiovert 40 C for transmitted light, fixed camera port, 5-position nosepiece, stage height 185 mm Axiovert 40 CFL for transmitted light, fixed camera port, 5-position nosepiece with 3 slots for PlasDIC sliders, stage height 210 mm, upgradable with PlasDIC and fluorescence Contrasting techniques Axiovert 40 C: brightfield, phase contrast, VAREL Axiovert 40 CFL: brightfield, phase contrast, VAREL, PlasDIC and reflected light fluorescence Objectives Eyepieces CP-Achromat, A-Plan, LD A-Plan, Achroplan, Plan-Neofluar, LD Plan-Neofluar PL 10x/18 Br., 10x/18 Br. foc. E-PL 10x/20 Br., 10x/20 Br. foc. Stages Documentation specimen stage, specimen stage glass, heatable and temperable microscope stages Cameras: SLR, digital compact, digital cameras from the AxioCam family, video Adapters: for many models of cameras Software: camera software, AxioVision 13

48 The Plus Points. Your Benefits at a Glance. ++ Designed to satisfy your requirements: the Axiovert 40 inverted microscope for routine applications in cell and molecular biology ++ Leading optics: uncompromising Carl Zeiss quality ++ Easy operation: ergonomic design, variable viewing height thanks to the Siedentopf principle ++ Innovative technique: PlasDIC, the differential interference contrasting technique for routine applications ++ Brilliant fluorescence: new 3-position slider Push&Click for greater elimination of stray light ++ Efficient illumination: 35 W halogen lamp with more than a 800-hour lifetime ++ Optimal view: the optional specimen stage glass with free view of nosepiece ++ Exceptional stability ++ Sturdy: long-lasting, robust mechanics ++ Flexible upgrading: ready for future demands e Carl Zeiss Light Microscopy P.O.B Göttingen GERMANY Phone: Telefax: micro@zeiss.de Subject to change Printed on environment-friendly paper, bleached without the use of chlorine.

49 Microscopy from Carl Zeiss Primo Star iled Fast. Versatile. Reliable. The Flexible, Complete Solution for Testing Tuberculosis and Other Infectious Diseases Using LED-Fluorescence and Brightfield

50 The Fight Against Tuberculosis Has a Long and Successful Tradition. Using a Carl Zeiss microscope, in 1882 Robert Koch discovered the bacterium that causes tuberculosis: Mycobacterium tuberculosis. Today, technology from Carl Zeiss is still making a valuable contribution in the fight against infectious diseases: Primo Star iled, an LED-based fluorescence microscope, is fast, sensitive, robust, and easy to operate. Robert Koch s discovery was made possible by using the 100x objective with homogeneous oil immersion, calculated for the first time by Ernst Abbe The LED-based fluorescence microscope Primo Star iled simplifies the microscopic analysis of pathogens A successful collaboration: FIND and Carl Zeiss Primo Star iled is the result of a joint project between the Foundation for Innovative Diagnostics (FIND) and Carl Zeiss. Through the targeted promotion of diagnostic innovations, the Swiss foundation FIND has set itself the objective of combating the spread of dangerous infectious diseases. At Carl Zeiss, the battle against these diseases has a tradition dating back to Robert Koch s groundbreaking discovery. To this day, the company continues to set the standard with commitment and expertise Primo Star iled: the result of a successful collaboration between FIND and Carl Zeiss in the struggle against deadly infectious diseases. Its efforts in this area have enabled Carl Zeiss to come up with the LED-based fluorescence microscope Primo Star iled, developed specifically for tuberculosis testing and based on the powerful routine microscope Primo Star. The success of this collaboration between FIND and Carl Zeiss is plain to see: Primo Star iled will be offered at a particularly favorable price to public health services customers in the 22 high burden countries as defined by the WHO. Tuberculosis still a deadly threat Despite the fact that tuberculosis can be treated with antibiotics, cases of the disease are on the rise. In the statistical tables of deadly infectious diseases, it is ranked at the top alongside AIDS and malaria. Today, one in three people are already infected. The development of multiresistant strains and co-infection with the HIV virus are giving rise to alarming prognoses: according to estimates by the World Health Organization (WHO), this situation will claim in the region of 30 million human lives around the world over the next ten years. An important component for fast and reliable testing The further spread of tuberculosis can only be prevented by means of efficient testing, followed by the appropriate treatment of those affected. For this reason, the WHO recommends that Mycobacteria should be examined using fluorescence microscopy. The LED-based Primo Star iled fluorescence microscope combines the advantages of fluorescence microscopy and, thanks to a number of innovative elements, sets new standards in robustness, energy efficiency, and simple operability. Primo Star iled a further development of Primo Star is impressive with its versatile performance features: Reflected-light fluorescence Rapid switching from fluorescence excitation to brightfield illumination Economical LED concept Battery pack for operation without a main power supply Special eyecups eliminate the need for a dark room Simple to operate Durable and robust Tried-and-tested Carl Zeiss optics made from high-quality glass High-quality materials Worldwide support from Carl Zeiss In addition, Primo Star iled offers all the advantages of Primo Star: Ergonomics: swiveling Siedentopf tube with comfortable viewing angle of 30º, adjustment for individual interpupillary distances from 48 mm to 75 mm Flexibility: modular and easy changeable illumination concept: halogen light (30 W), highly efficient, color-temperature stable LED, or tilting mirror (Fixed- Koehler) Convenience: external power supply unit with integrated storage position Form and function: attractive design combined with maximum functionality, intensity indicators on both sides of the microscope Greater performance wider range of applications Primo Star iled is also suited to test for other infectious diseases such as malaria and sleeping sickness. The ability to easily switch from fluorescence to brightfield also makes it possible to use the microscope for all simple laboratory and routine applications. All at a price-performance ratio that is unequaled in this segment. 2 3

51 Simply Better Performance with LED Fluorescence in Reflected-light: 10 % Increase in Sensitivity of Tuberculosis Testing. Speed and sample throughput are essential success factors in the treatment of tuberculosis. After all, fast and reliable testing means early treatment. Tried-and-tested standard: pathogen analysis in brightfield The analysis of Mycobacteria using the transmitted-light brightfield technique is still common practice in the area of tuberculosis testing and it is possible to use this illumination technique with Primo Star iled too. Due to the small size of the Ziehl-Neelsen-stained disease pathogens, in this case it is often necessary to work with 100x objectives. At such an objective magnification, however, the object field that the user is able to see is correspondingly small. The consequence of this is that it is comparatively time-consuming to examine the sample. Better performance on every point: the LED-based fluorescence contrast of Primo Star iled Under the fluorescence excitation of Primo Star iled, Mycobacteria appear yellowy green against a dark background. As a result, and by using a 40x objective with a larger object field, the pathogen can be detected up to four times faster. In addition, the sensitivity of pathogen detection is also increased by 10 %. The fluorescence excitation takes place using Auramine O, which is excited by blue LED light (not UV light!) with a wavelength of 455 nm and emits from 500 nm to 650 nm. The excitation is perfect and produces excellent contrast with an outstanding signal-to-noise ratio. Brightfield and fluorescence with Primo Star iled anything is possible Simple switching from fluorescence to brightfield means that Primo Star iled can be used with considerable versatility. Depending on your requirements, it is possible to employ brightfield transmitted-light techniques or fluorescence techniques. This makes Primo Star iled the complete solution for LED-based tuberculosis testing using conventional methods or fluorescence. Flexible and powerful true Carl Zeiss quality. Dr. med. Harald Hoffmann Head of the Institute for Microbiology and Laboratory Diagnostics, Asklepios Fachkliniken, Munich-Gauting Primo Star iled is the much longed-for answer to the three most pressing problems faced by tuberculosis microscopy in our partner countries. 1. The microscope offers fluorescence and transmitted-light brightfield microscopy in one, but does not require bulbs to be changed or adjusted nor Köhler illumination to be set up. The change between fluorescence and brightfield illumination is achieved with a simple flick of a switch. When a microscope can be operated as easily as this, training microscopists is made a great deal easier. 2. In addition to the favorable purchase price, Primo Star iled also impresses by reducing the running costs of fluorescence microscopy from more than a dollar Carefully conceived details for use under the most difficult circumstances A variety of innovative details have been developed to ensure that the microscope is easy to operate and can be used flexibly. With just a small number of manipulations the instrument can be used either as a brightfield microscope or fluorescence microscope. The use of LEDs is not only cost-effective: compared to mercury-vapor lamps as a light source for fluorescence, LEDs offer many application-oriented advantages. an hour to less than half a cent. This has been made possible by swapping the expensive mercury-vapor lamp for low-cost, long-life light-emitting diodes. Consequently, fluorescence microscopy, which is better suited for diagnostic purposes, is brought within everybody s reach in terms of affordability. 3. Primo Star iled needs hardly any electricity and can be run on batteries for many hours. This means that tuberculosis microscopy can also be offered in regions where a reliable power supply is not guaranteed. Primo Star iled is a handy and robust allrounder with an attractive design. It is sure to become a permanent feature of testing in our partner countries. For use in the field and in regions with a fluctuating power supply a battery pack has been developed which allows the fluorescence microscope to be operated for several hours without main power. The special eyecups almost completely block out ambient light, rendering a dark room unnecessary. Impressive in every detail all in all, this is a carefully conceived solution equipped for the most difficult working conditions. Mycobacterium tuberculosis. Analysis following Ziehl-Neelsen staining: the violet-stained mycobacteria can only be seen with difficulty in the microscopic image Using the fluorescence contrast technique, the Mycobacteria can be identified easily, after being stained with Auramine O, as luminous yellowy green particles against a dark background From fluorescence to brightfield contrast with the flick of a switch The battery pack makes it possible to work without main power 4 5

52 A Wide Range of Applications Means Investment Security: Primo Star iled Offers More Possibilities for Testing and Makes Testing Faster. Quick intervention is of the utmost importance and what goes for tuberculosis testing goes for other infectious diseases too. According to the WHO, million people are taken ill with malaria and sleeping sickness each year. For these diseases too, Primo Star iled allows fast and reliable testing and, consequently, gives patients a good chance of recovery. Primo Star iled can be used universally anywhere in the world and is also suitable for routine analyses in medical practices The worldwide spread of tuberculosis Malaria a threat not only in tropical Africa It is estimated that malaria, caused by plasmodia (singlecell parasites), claims the lives of 1 million people worldwide every year. If patients are tested in good time, all forms of malaria can in principle be cured. Microscope technology that is as fast as it is reliable is an important factor in making this possible. Meaningful and reliable: microscopic analysis with Primo Star iled Malaria pathogens can be detected in brightfield with the help of the Primo Star iled, as this makes the various stages of maturity of the plasmodia visible. What is more, it is also possible in principle to perform fluorescencebased analysis using e. g. Acridine orange. Malaria tropica, the most serious form of the infection, is caused by Plasmodium falciparum Malaria tertiana is caused by Plasmodium vivax and Plasmodium ovale Malaria quartana results from Plasmodium malariae The Primo Star iled image, which is so rich in contrast, allows the characteristic structures of the individual malaria pathogens to be identified clearly. Any changes to the infected erythrocytes are made visible using objectives with magnifications of 40x to 100x. The various stages of maturity can also be detected. From the early stage of ring forms, via the daisy-head stage, through to rosette formation: the outstanding optics from Carl Zeiss deliver a brilliant image for hematology. Testing for sleeping sickness Primo Star iled is also for carrying out tests for sleeping sickness (Human African Trypanosomiasis), an infectious disease caused by trypanosomes that mainly occurs in tropical Africa. According to estimates by the WHO, a total of more than 500,000 people are affected by it. The disease is transmitted by the tsetse fly, the bite of which allows flagellates to enter the human bloodstream. The pathogens are: Trypanosoma brucei gambiense in Western and Central Africa Trypanosoma brucei rhodesiense, primarily in Eastern Africa Trypanosoma cruzi (Chagas disease) in South America In this case too, the chances of recovery are increased by quick testing. Primo Star iled allows the microscopic detection of pathogens, e.g. in a blood smear or in spinal fluid sediment: the spindle-like form of the trypanosomes, the long flagella and the undulating membrane can be identified clearly and with high contrast. Other routine medical applications Primo Star iled is also extremely well-suited to other laboratory-based and routine medical applications involving fluorescence-based testing and brightfield illumination. You will also find it ideal for tests in the field of veterinary medicine. Somsak Rienthong Senior Medical Scientist and chief of NTRL, National TB Reference Laboratory, Bureau of Tuberculosis, Bangkok, Thailand The new iled excitation allows fluorescent tuberculosis pathogens to be made visible using an ordinary light microscope compared with conventional fluorescence microscopes it is a very economical solution and offers high sensitivity. This is a huge advantage for tuberculosis control. The WHO and other organizations fighting TB are extremely hopeful that a robust and powerful fluorescence microscope will make it possible to improve TB testing, especially in the case of HIVinfected patients ) Plasmodium malariae, daisy-head stage, 2) Plasmodium vivax showing characteristic Schüffner s dots, 3) Trypanosoma brucei gambiense with undulating membrane Andrea Michelsen, Managing Director of the Deutscher Verband Technischer Assistentinnen/Assistenten in der Medizin e.v., Head of the Central Laboratory of Ortenau Klinikum Lahr-Ettenheim, Germany Worldwide presence The Carl Zeiss network your guarantee of outstanding service. Besides a fast spare-parts and repair service, this also means that you will receive support from an experienced, international team of experts on all questions relating to microscopy. Whether you get in touch over the Internet, via the hotline or through your personal contact, you will obtain expert advice and help in finding the right solution for your specific application whether in education or in the lab. Prof. Dr. Dr. Knut Feldmann President of the Tuberculosis in the World Foundation, Advisor to the WHO and Secretary of the Bacteriology/Immunology Section of the International Union Against Tuberculosis and Lung Disease 6 7

53 Primo Star iled: High-performance Technology in its Most Compact Form, Right Down to the Last Detail. Technical Data: Primo Star iled * The Primo Star iled complete solution and basic Primo Star stand consist of a whole range of sophisticated elements, ensuring reliable results for users world-wide. An economical solution: the LED concept The innovative LED concept for fluorescence excitation and illumination is particularly economical and energy efficient. With a lifespan of at least 10 years, the LEDs offer unrivaled durability: over the average life of a microscope, there will be most likely no need to change the LEDs. Operation is extremely simple for the user: No need for lamp changing and complex adjustment Primo Star iled is ready to use immediately, without the warm-up and cool-down times required with mercuryvapor lamps No danger of being light-dazzled, because of reflected-light fluorescence LEDs are an energy-saving option: they consume onetenth the power of a 50 W mercury-vapor lamp Hardly any heat is generated, samples are protected Modular architecture: iled as a retrofit package If you already own a Primo Star, you can easily retrofit Primo Star iled. The retrofit package is comprised of a fluorescence module, carrying handle, magnet insert, eyecups, and diaphragm slider. It can be assembled easily without any need for time-consuming alterations to the microscope. High-quality, well-protected Carl Zeiss optics An antifungal treatment has been applied to all optical components. This coating ensures that they can be used optimally even under extreme climatic conditions and offers long-lasting protection. All the optics have been manufactured from high-quality glass that complies with the very highest quality requirements. Also, security has not been overlooked either: objectives and eyepieces are protected against theft both during use and transportation. The impressive compact Primo Star iled microscope with its various details A retrofit package allows the basic Primo Star microscope to be converted into a Primo Star iled Technical data: Primo Star iled complete package Optical system Parfocal distance Tube length Magnifications Eyepiece tubes Eyepieces Nosepiece Objectives Condenser Illumination Stage Area of stage Travel range Z-drives Weight Accessories Color-corrected infinity optics 45 mm 180 mm 100x to 1000x for visual observation Swiveling Siedentopf tube with upper and lower position: upper position offers approx. 40 mm extra viewing height; adjustable interpupillary distance: 48 mm to 75 mm; tube can be rotated 360 ; 30 viewing angle (ergonomic angle); binocular tube WF 10x/18 Br. foc. with special eyecups 4x, inclined backwards Plan-ACHROMAT 10x/0.25 D=0 WD: 4.51 mm; Plan-ACHROMAT 20x/0.4 D=0 WD: 1.00 mm; Plan-ACHROMAT 40x/0.65 D=0 WD: 0.45 mm; Plan-ACHROMAT 100x/1.25 D=0 WD: 0.30 mm Abbe condenser 0.9/1.25 (Fixed-Koehler) Modular illumination concept with drawer for transmitted-light illumination; white-light LED (3 W/6 V); blue-light LED (455 nm; 3 W/6 V); LED class 3B; reflected-light fluorescence Stages with right-hand operation w x d: 140 mm x 135 mm w x d: 75 mm x 30 mm Fine drive: 0.5 mm/rot.; Coarse drive: 45 mm/rot.; Total travel range: 15 mm Approx. 9.6 kg Eyepiece pointer, battery pack, binocular phototube 50 % : 50 % (vis:doc) Tilting mirror (only for Fixed-Koehler stand variant) Phase contrast equipment: 10x/Ph1; 20x/Ph2; 40x/Ph2; 100x/Ph3; HAL (30 W/6 V), with phase sliders Darkfield equipment (DF slider 0.65) Camera adapters: C-mount adapter 1/2 ; C-mount adapter 2/3 ; digital camera adapter AxioVision LE with Canon camera driver European standards: CE for safety and for electromagetic radiation, IvD 98/79/EG. International standard: CSA. Quality Management: ISO 9001, ISO Environmental management: ISO * only mm in the rotated position Antifungal guarantee To provide an antifungal coating, Carl Zeiss treats all optical components with a special chemical from Bayer. Retrofit guarantee The iled module can be easily retrofitted onto all existing Primo Star instruments with just a few manipulations. 8 9

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55 Carl Zeiss MicroImaging GmbH Jena, Germany BioSciences Göttingen Location Phone: Telefax: Information subject to change. Printed on environmentally friendly paper bleached without cholorine /e printed

56 Microscopy from Carl Zeiss Primo Star Greatness is a Question of Opportunities. Power. Performance. Value. Invest in the Best in Education, the Laboratory, Practice and the Field.

57 Primo Star. High Performance for Education and the Laboratory. They have to be robust, easy to operate, equipped with good optics and, above all, reasonably priced: the demands placed on educational microscopes are very specific. The constant assembly, disassembly, and handling by inexperienced microscope users as an everyday course element requires the microscope to be extremely durable and its operation to be straightforward. Carl Zeiss has responded to these demands and, with Primo Star, is presenting a new generation of instruments in educational microscopy a microscope that has been developed with long-term use and great durability in mind. Robust, with a fresh design and ergonomic viewing. Primo Star incorporates all the experience gathered by Carl Zeiss in the field of light microscopy, adapted specifically for educational purposes. Easy to operate Robust and durable Quality optics from Carl Zeiss Numerous innovative solutions Flexible through its modularity Excellent price/performance ratio Primo Star is available in ten different variants. The right version for every set of requirements. This microscope together with its many practical accessories can be used for education, in the laboratory, doctors practice and in the field. Genuine Zeiss. 2

58 Primo Star 3

59 Incredibly Practical. Innovative Solutions in Their Most Attractive Form. Primo Star is more than just an educational microscope: behind its stunning design lies an intelligent functionality. In close cooperation with teaching institutions, Carl Zeiss has developed unique components specifically for Primo Star. From the modular illumination concept and ergonomic Siedentopf tube through to the illumination intensity display, Primo Star impresses with its numerous well thought-out details. For your students this means maximum convenience as they learn to use a microscope. And, for you, that wonderful feeling of having a genuine Zeiss right from the start. Carrying handle: safe and secure Maximum safety for the microscope: integrated carrying handle Plastic-coated on the inside Once dismantled, convenient and safe transport in microscopy course rooms and laboratories Modular illumination: cost-efficient or standard? Three illuminations for educational and lab work 30 Watt halogen light LED with stable color temperature and higher efficiency as the most economical solution, and for long-term usage Tilting mirror for operation without electrical power Comfort for all users: the Siedentopf tube Unique and comfortable in the laboratory and for educational use: swiveling Siedentopf tube Ergonomic viewing angle of 30º Ability to adjust viewing height to users height Wide adjustment range for individual interocular distance (48 mm to 75 mm) No untidy cables: the external power supply unit Special rear panel with external power supply unit and cable Adapter for conversion to a table-top power supply unit In the event of repair, just the power supply unit is replaced Modular illumination 1 4

60 4 Performance Swiveling Siedentopf tube Total control: the light display Blue light intensity display on both sides of the stand Important control function allowing lecturers to check all microscopes in the course room, even from a distance Quick control of light intensity by the user Practical: stages with right-hand or left-hand drive operation For laboratory and educational practice: righthand and left-hand stage drive operation Operation of stage drive using the right hand and focus drive using the left hand, or vice versa Individual set-up of the laboratory or course room Form follows function: the design Innovative design: combination of aesthetics with maximum functionality Quality and high standards with regard to the choice of materials: mostly metal, minimum use of plastic Functional and stable Carrying handle 3 Power supply unit with plug adapters 2 5

61 Quality Prevails. Worldwide. Wherever you are you are never far from Carl Zeiss. When it comes to service and sales, the Carl Zeiss network is unequaled worldwide. Our comprehensive technical support network guarantees rapid help and maximum availability in numerous countries across the world. The advantage for you: your personal partner can reach you quickly, when it matters. Whether you are in Asia, Europe or the USA, and whether it s for education, laboratory or field work Carl Zeiss microscopes are held in high esteem the world over. Like Primo Star. With fresh ideas and the latest technology. Global presence The Carl Zeiss network your guarantee for outstanding service. Besides a speedy spare part and repair service, this also means support with regard to all microscopy-related issues from an experienced international team of experts. Via the internet, hotline or your own personal contact, you will encounter specialist advice and always find the right solution for your special application in education, the laboratory or doctors practice. Primo Star used internationally Primo Star is a versatile microscope that can be used for anything that is relevant to education and laboratory operation. All the functions and operation can be quickly learned. The ergonomic adjustment of the viewing height, country-specific plugs and option of use in the field have been designed for international usage. For this reason, it is suitable for education and routine work in all areas of microscopy, in all corners of the globe. 6

62 International Learning microscopy made easy Designed specifically for education, Primo Star covers the various educational stages of microscope use. Starting from useful magnification through to a perfectly adjusted microscope according to Koehler Primo Star makes learning how to use a microscope as simple as can be. Thanks to its easy operation and robustness, Primo Star is particularly suitable for use in schools. Even sophisticated work flows like those at schools for cytology assistants can be quickly explained and learned with Primo Star. Special packages are also available for training facilities at higher education institutions for medical occupations and at medical and biological university faculties. This way, even the Koehler method becomes an easy exercise. For the required contrast methods and preparations Using Primo Star, it is possible to use all the applications and contrasting methods that are relevant to education. Stained tissue sections in medicine Unstained cells in phase contrast in medicine and biology Plant stem cross sections in botany Extremely fine structures such as diatoms in darkfield in biology Examination and analysis of germs and bacteria in field work 7

63 More Possibilities. The Right Primo Star for Every Requirement. Carl Zeiss has developed ten packages for Primo Star which are geared entirely toward the everyday routine work of education and laboratory practice. The high Carl Zeiss standard of quality is common to all variants. The difference lies in the detail: depending on the level of education and requirement profile, long-term in this area is variant 3 with LED illumination. If a constant color temperature is important to you and you intend to use Primo Star for a long time, then this is the instrument to choose primarily because of its extremely long service life and low operating costs. the individual packages differ slightly in what they offer. This means that you get exactly what your daily practice calls for. For standard microscope work at the highest level: the Full-Koehler versions Packages 4-8, which are used to teach the classic Fixed-Koehler versions for standard education Primo Star packages 1-3 these microscopes mark the new standard in education. They have all the important functions and are very simple to operate. In addition, we offer the stage with left-hand operation Koehler method, are suitable for higher level education. These Primo Star packages also have a somewhat larger field of view, the option of left-hand operation and a phototube for connection to a digital or video camera. They are also available with the cost-efficient LED illumination as an option. for all those who wish to use the microscope with their left hand and write or draw with their right hand. The most economical solution in the a. Sliders and filters Fixed-Koehler package Variant with phototube b. AxioCam HR c. Digital camera a. b. 8 c.

64 1. Fossil foraminifera, annular-diaphragm darkfield Objective: Plan-Achromat 40x 2. Waterweed (Elodea), phase contrast Objective: Plan-Achromat 40x 3. Flower umbel of the daisy (Bellis perennis) Brightfield view Objective: Plan-Achromat 10x With the help of AxioVision microscope software, the whole world of camera technology is open to you: TV cameras, digital cameras and the special AxioCam microscope cameras from Carl Zeiss. Using the AxioVision LE software, an extremely good value solution, immediately presents itself with regard to digital, interactive image analysis and documentation for select digital cameras from Canon. This solution is available specifically for education and simple laboratory applications. Primo Star with phase contrast Primo Star packages 9-10 In biology in particular, phase contrast is frequently used in education in order to make the finest structures of unstained cells visible. This is a particular challenge for educational microscopes. For Primo Star, however, it is a matter of course. As an option even with darkfield contrast, to make the smallest structures visible. AxioVision Primo Star packages Package Stage drive Stage drive Field of view Field of view Fixed- Full- HAL LED Ph2 Photo- Requirements/ no. right left 18 mm 20 mm Koehler Koehler (40x) tube target segments Standard education, e.g. 1 X X X X simple course room applications as no. 1 but with 2 X X X X left-hand operation as no. 1 but with 3 X X X X LED illumination Higher level of education, e.g. 4 X X X X universities, laboratory as no. 4 but with 5 X X X X X phototube as no. 5 but with 6 X X X X X left-hand operation as no. 5 but with 7 X X X X X LED illumination as no. 7 but with 8 X X X X X left-hand operation 9 X X X X X Higher level of education 10 X X X X X X as no. 9 but with phototube 9

65 10 Please refer to the current national price lists for the order numbers and prices for accessories.

66 System Overview 11

67 Performance in Detail. * Technical Data *only mm in the rotated position Optical system: Parfocal distance: Tube length: Two stand variants: Magnifications: Eyepiece tubes: Eyepieces: Nosepiece: Color-corrected infinity optics 45 mm 180 mm Full-Koehler and Fixed-Koehler 40x to 1000x for visual observation 4x to 100x for camera observation Swiveling Siedentopf tube with upper and lower position: upper position offers approx. 40 mm extra viewing height Adjustable interpupillary distance: 48 mm-75 mm Tube can be rotated 360º 30º viewing angle (ergonomic angle) Binocular tube and binocular phototube 50%:50% (vis:doc) Several photo adapters available for phototube WF 10x/18 Br. foc. WF 10x/20 Br. foc. Eyepiece pointer can be retrofitted, all eyepieces anti-fungus treated 4x, inclined backwards 4x, reversed Objectives: Plan-Achromat 4x/0.1 WD: 6.50 mm Plan-Achromat 10x/0.25 WD: 4.39 mm Plan-Achromat 40x/0.65 WD: 0.48 mm Plan-Achromat 100x/1.25 WD: 0.13 mm Plan-Achromat 40x/0.65 Ph2 WD: 0.48 mm Condenser: Abbe condenser 0.9/1.25 Holder for phase contrast and darkfield sliders Two variants for fields of view 18 mm (Fixed-Koehler) and 20 mm (Full-Koehler) Illumination: Modular illumination concept with drawer HAL (30 W/6 V), LED (3 W/6 V, roughly corresponding to brightness of 20 W HAL) Stage: Stages available with either right-hand or left-hand operation Area of stage: w x d: 140 mm x 135 mm Travel range: w x d: 75 mm x 30 mm Z-drives: Fine drive: 0.3 mm/rot. Coarse drive: 4 mm/rot. Total travel range: 15 mm Accessories Carrying case, microscope mat, eyepiece pointer Tilting mirror (only for Fixed-Koehler stand variant) Phase contrast equipment (Plan-Achromat 40x/0.65 Ph2 + Ph2 slider) Darkfield equipment (DF slider 0.65) Camera adapters: C-mount adapter 1/2 ; C-mount adapter 2/3 ; digital camera adapter AxioVision LE with Canon camera driver Norms and standards met: CE, CSA, UL, ICC, ISO 9001, IvD All optical components are anti-fungus treated Carl Zeiss MicroImaging GmbH P.O.B. 4041, Göttingen, Germany Phone: Fax: micro@zeiss.de Dealer s stamp Subject to change. Printed on environmentally-friendly paper, bleached without the use of chlorine e

68 M i c r o s c o p y f r o m C a r l Z e i s s Primo Vert Affordable. Easy-to-Use. Ergonomic. The inverted microscope Primo Vert is focused on the essentials: Excellent optical quality, ease of use, choice of high quality materials and an appealing design are all available at an affordable price. Primo Vert addresses both routine and cutting-edge research laboratories which require compact and reliable microscopes for checking cells quickly and efficiently. The microscope can be also used by students in advanced course room settings. Key customer benefits at a glance: Excellent price-performance ratio Easy-to-Use concept: auto on/off function, universal phase contrast Long-term mechanical and optical stability Attractive industrial design In addition to the binocular tube and trinocular tube (photo) versions, Primo Vert offers an innovation that generates a new class of its own: The Primo Vert Monitor. Ultimate convenient: Primo Vert Monitor Monitor allows multi-observation in training situations Adjustable monitor tilt (45-80 ) for ergonomic and comfortable work Head is no more in a fixed position to the eyepieces; glasses are no longer a problem Integrated digital camera, SD memory card slot, USB port Remote control for the acquisition of images, remotely or through glass plates of flow boxes The new fast and convenient solution for assessing and checking living cells.

69 Focused on Essentials Ease of Use and Reliable Results Primo Vert presents a number of attractive details to guarantee quick, easy and reliable handling. This makes Primo Vert the perfect tissue culture phase contrast microscope. The walk away mode turns off the light automatically (in addition to manual on/off-function) after 15 minutes to save energy and prolong lifetime of the bulb. Modular illumination with halogen light or with longer lasting LEDs. The advantages of LEDs are the stable colour temperature, only one tenth in power consumption, significantly longer lifetime therefore a lower cost solution. Special Skin-friendly material coated on all operating elements. Universal phase slider for all objectives: For ease of use there is one phase ring (Ph 1) available for 10x, 20x and 40x this means no need to change phase positions when switching magnifications. Ease of use: Walk away mode and light push-button on the stage makes a quick check of living cells more than easy. Increased working distance through easy removal of the condenser, e. g. for roller-bottles. Objective-indicators for fast identification of magnification. Carrying handle on the back of the scope. High-quality optics through high-quality glass. Objectives with infinity optics for magnification up to 40x long working distance provide high-contrast and brilliant images. Swivelling Siedentopf-tubes. The viewing height can be adjusted to an upper or a lower viewing height according to the preferences of the user. The inter-pupillare distance can be adjusted down to a minimum of 48mm. Increased working distance through easy removal of the condenser, e. g. for use of roller-bottles. One example of the numerous applications is molecular oncology Cancer cells are cultured in incubators for the purposes of research. Their stage of development can easily be assessed with Primo Vert a routine step that requires a consistent workflow and fast results. The microscopic image must provide absolutely reliable information on whether the cells are adhering to the bottom of the vessel, which means they are intact and can be used in further experiments. On the other hand, if they are floating in the nutrient medium, they are stressed or already dead and thus unfit for further use. U2OS cells: Magnification 4x Ph0 U2OS cells: Magnification 40x Ph2 Hela-cella: Magnification 20x Ph2 2

70 The Primo Vert Monitor A New Viewing Perspective With the new Primo Vert Monitor*, Carl Zeiss offers an ideal microscope for multi-observation purposes. Several users can look at and discuss the microscope image at the same time without having to adjust any eyepieces individually. The new pass by mode in microscopy. Just walk and check your cell lines in the pass by mode: Easy inspection of living cells is possible, i.e. without having to look through eyepieces. Ergonomic quick check of living cells. The monitor can be tilted from 45 up to 80. So users can easily adjust the LCD monitor to their individual posture. Convenient image storage by the push-to-save camera to SD memory card without PC connection. Additionally a computer can be connected to the USB port on the stand. No requirement to remove eyeglasses in order to check the microscopic images. Primo Vert enables discussion of the microscope image by several users. The main applications for Primo Vert are: Sterility checks Cell checks before protein, DNA or RNA preparation Cell checks in the framework of screenings after donation of substances (e. g. pharmacology) Differentiation of cell types Characterisation of cell lines (oncology) Growing of cells to produce artificial tissues or organs Documentation of the above test results *= For research only - not for medical diagnostic. The microscope image of the embedded camera can easily be stored on the SD card. All Primo Vert versions fit well into hoods and flow boxes. The image acquisition of Primo Vert Monitor can optionally be operated by remote control.

71 Primo Vert Optical and Mechanical Data Objective change Objectives Manual via quadruple objective nosepiece Infinity-corrected objective range with W 0.8 mounting thread Plan-Achromat: 4 x / 0.1, 4 x / 0.1 Ph0, 10 x / 0.25 Ph1 LD Plan-Achromat: 20 x / 0.3 Ph1, 40 x / 0.5 Ph1, 20 x / 0.3 Ph2, 40 x / 0.5 Ph 2 Phase-Slider Universal phase slider for the objectives Ph1: Convenient and economical Phase slider for Ph2: Higher resolution Eyepieces Specimen stage Dimensions (width x depth) Specimen guide Verniers with numerical and alphabetic scale Coaxial drive LD condenser 0.3 LD condenser 0.4 Binocular tube 45 /20 Interpupillary distance Viewing height Trinocular (photo)tube 45 /20 Photo/video port Fixed beam splitting WF-PL 10x/20 Br. foc. Fixed 200 x 239 mm Right side X direction: Numerical scale, readable from right to left Y direction: Alphabetic scale, readable in the mirror right side For magnifications 4 x to 40 x, WD = 72 mm For magnifications 4 x to 40 x, WD = 55 mm Viewing angle 45, FOV 20 Adjustable from 48 to 75 mm 360 to 397 mm Viewing angle 45, FOV 20 Tube factor 1x, 60 mm 50 % vis / 50 % doc Primo Vert Monitor Camera: 5 Megapixel CMOS Monitor size: 8.4 Display: 800 x 600 pixel Storage Medium: Secure Digital (SD) card Outputs/ports: USB 2.0 Camera driver for AxioVision LE with special Microscopy software: Configuration tool Light source HAL: 6 V, 30 W LED: White light, 3 W All optics in Primo Vert are anti-fungus treated Norms and standards met: CE, UL, CSA, IvD, DIN EN (IEC ), ISO 9001 Available optional accessories: Object guide and several mounting frames for Petri dishes etc., stage inserts (metal or glass), stage enlargements, several camera adapters, eyepiece micrometer and eyepiece pointer, neutral density and green interference filters, cameras, AxioVision LE microscope software Carl Zeiss MicroImaging GmbH Jena, Germany BioSciences Göttingen Location Phone : Telefax : micro@zeiss.de Information subject to change. Printed on environmentally friendly paper bleached without chlorine /e printed 04.10

72 Microscopy from Carl Zeiss Stemi 2000 Stereomicroscopes Best Optics High Resolution Super Zoom

73 Stemi Superior through Zeiss Perfection Stemi 2000 stereomicroscopes meet your expectations in two respects: They offer the cost advantage of the Greenough design, while boasting surprising performance features. The ultimate in 3D imaging Thanks to their excellent optics, Stemi 2000 stereomicroscopes provide brilliant, sharp, distortion-free and highly resolved images even at the important low magnifications. Spectacular zoom factor of 7.7 If you are keen on minute structures, you will enjoy the 7.7:1 zoom range. Zoom in on details, either continuously or by click stops. The commanding overview The wide field of view already offered by the basic configuration lets you overview an object area greater than 35 mm in diameter. The 0.3x supplementary lens expands this to 118 mm. 2 Quartz crystals Burdock burr

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75 A Stemi 2000 triplet Stemi 2000 comes in three body versions to choose from, depending on your requirements: Stemi 2000 for stereoscopic observation only Stemi 2000C with photo- or videocamera port and 100/100% beam switch Stemi 2000CS with photo- or videocamera port and fixed 50/50% beamsplitter Modular and flexible The modular construction of the Stemi 2000 stereomicroscopes with their sensibly designed interfaces permits a broad spectrum of uses. Select from stands, mounting brackets and stages to configure exactly the stereomicroscope you require. Light and light are not the same Allowing epi-, transmitted and mixed illumination techniques in bright- or darkfield, the Stemi 2000 system includes various fiber-optic illuminators and cold-light sources of different wattages to suit different applications. Stemi 2000 with darkfield epi-illumination and high-output KL 2500 LCD cold light source. Added option: Remote control of KL. An advantage of the 0.3x...0.5x supplementary zoom lens: It varies the viewing height within ± 70 mm. Specially suited to go with cantilever and hinged-arm stands. Inspecting and archiving The Stemi 2000C and Stemi 2000CS models can be connected with the AxioCam MRc5 digital microscope camera or commercial digital photoor videocameras. The Stemi 2000CS with its builtin beamsplitter allows you to do observation and documentation simultaneously. Stemi 2000C on the DA stand for inspecting large specimens. Professional documentation with the AxioCam MRc5 pin-sharp and absolutely true to color. 4

76 Stemi 2000 Stereomicroscope with KL 200 Cold-Light Source Stemi 2000 Stereomicroscopes With built-in Zeiss excellence. Carl Zeiss Stemi 2000 Stereomicroscopes are indisputably among the best in their class. Their excellence in contrast, depth of field and resolving power makes them the number one choice for inspecting three-dimensional objects in nature and technology, as well as in specimen preparation for research and teaching. Movement of a watch There is hardly a field of application in which the superior brilliance of Stemi 2000 images would not prove useful. 5 Grass seed Printed circuit board Tick Textile fibers

77 Specification Supplementary lens Eyepiece Factor Free WPL 10x/23 Br. foc WPL 16x/16 Br. foc W 25x/10 foc working distance (mm) Magnification Object field Magnification Object field Magnification Object field (mm) (mm) (mm) 0.3x x 15.0x x 24.0x x 37.5x x 0.5x x 25.0x x 40.0x x 68.8x x x 20.0x x 32.0x x 50.0x x x 31.5x x 50.4x x 78.8x none x 50.0x x 80.0x x 125.0x x x 62.5x x 100.0x x 156.3x x x 80.0x x 128.0x x 200.0x x x 100.0x x 160.0x x 250.0x Stemi 2000 on stand S Weight: 4.2 kg Carl Zeiss Light Microscopy P.O.B Göttingen GERMANY Phone: Telefax: micro@zeiss.de Subject to change. Printed on environment-friendly paper, bleached without the use of chlorine e/08.03

78 Microscopy from Carl Zeiss Stemi DV4 Stereomicroscopes The Essentials in Focus

79 For Education and Routine in Science and Industry Brilliant images and easy to use microscope functions make microscopy lessons a genuine pleasure for students and teachers alike. The fruit of the cocklebur uses hooks to attach itself to animals furs, which then disperse it s seeds. Stemi DV4 in the classroom High-quality optics and a variable LED illumination in a compact unit are important criteria when it comes to tasks in industrial quality control. On a circuit board, residues of fluxing agents can be clearly identified between the contacts. Stemi DV4 with tilting mechanism being used in quality control Thanks to true-color images, combined with the possibility of switching between and mixing reflected and transmitted light, rapid and precise diagnoses can be achieved in biological laboratories. Chicken embryos in transmitted-light darkfield Stemi DV4 in a developmental biology laboratory A fiberoptic reflected light spot illuminator integrated into the body of the Stemi DV4 SPOT ensures that specimens are always well illuminated whatever the position and direction of observation. Applying protective lacquer to a plaster model in a dental laboratory Stemi DV4 SPOT on stand U in a dental laboratory

80 The Essentials in Focus The innovative nature of this stereomicroscope can be seen not only in the color and unique, compact design of the instrument, but also in its attractive price a result of state-of-the-art production and assembly technologies. Sturdy, hard-wearing technology, very easy operation of all microscope funktions and no compromises whatsoever in the definition and brilliance of the microscopic images that is the best way to describe the Stemi DV4 stereomicroscopes from Carl Zeiss. The pancratic system, as the heart of the stereomicroscope, has a decisive influence on the optical performance of the overall system. The Stemi DV4 is the first instrument in its class with a mechanically corrected zoom system, a feature that was previously only incorporated into stereomicroscopes in higher price categories. The result: needle-sharp images across the entire zoom range.

81 Stemi DV4 stereomicroscope with sample carousel 16 A range of well-chosen accessories make the Stemi DV4 an even more attractive option for a whole range of applications. Thanks to the sample carousel 16, up to sixteen different specimens can be positioned under the microscope safely, quickly and extremely easily.

82 Stemi DV4 stereomicroscope The patented zoom system of the Stemi DV4 (Double Lens Vario, zoom factor 4) delivers images that are needle-sharp and, thanks to rigorous stray-light minimization, brilliant and rich in contrast. Across the entire magnification range, from 8x to 32x and across the whole field of view. Stemi DV4 SPOT stereomicroscope The fiberoptic cold-light illuminator additionally integrated into the microscope body of the Stemi DV4 ensures that the object field is fully illuminated at all times. The SPOT reflected light irradiating almost vertically on the specimen, provides a shadow-free, high contrast illumination.

83 Stand C LED: Combine Illumination Modes for Outstanding Results As good results in microscopy depend on efficient illumination, we have equipped the stand with a modern LED illuminator. At the touch of a button: rapid switching between illumination modes. You have a choice between the following standard features: - reflected-light brightfield - transmitted-light brightfield - mixed light In addition, there is an option for: - transmitted-light darkfield - polarization contrast in reflected and transmitted light This means that you have the common illumination modes in stereomicroscopy at your fingertips. You can decide which ones to use and how to combine them practically during observation it can all be done rapidly at the touch of a button. Stand C LED features an integrated LED illuminator for applications in reflected and transmitted light. This powerful daylight-quality illuminator shows specimens with high contrast in their natural colors. In transmitted light, six LEDs ensure bright, homogeneous illumination at any magnification, from overview to detail. 9

84 Using the example of a lacewing (Chrysopa carnea, dead specimen), the different illumination options that stand C LED offers can be clearly illustrated. The ready-for-use design of the Stemi DV4 stereomicroscope with full range of illumination options as standard Reflected-light brightfield A standard illumination mode on a stereomicroscope, used to illuminate the surface of opaque specimens. If a certain amount of transmitted light is added, the disruptive shadows beneath the specimen fade. Transmitted-light brightfield Also a standard mode. For observing transparent specimens and contours. If a certain amount of reflected light is added, structures on the insect s body also become visible. Transmitted-light dark field (optional) Extremely fine structures are illuminated in their natural colors against a dark background. If a certain amount of reflected light is added, structures on the insect s body also become visible. 7

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87 The Basis of Your Modular System Exact positioning Quick, simple and with no risk. Sample carousel 16, which can be retrofitted to stand C LED, allows you to position up to 16 different specimens with precision under the stereomicroscope. A click-stop mechanism ensures precise positioning under the microscope, whilst a low-reflection glass plate protects the specimens. Measurement at a glance Using the eyepiece micrometer you can read measurement values directly from the scale, either at the 8x overview or 32x detail magnification. The 8x/32x/18 eyepiece micrometer is retrofitted in the 10x standard eyepiece. Digital documentation For the documentation of microscopic images you can attach the camera to one of the two eyepiece tubes in place of the eyepiece. Digital compact cameras, SLR- or video cameras can be connected to the microscope quickly and easily using the appropriate eyepiece adapters. 10