Leica DM4000 B Leica DM5000 B. Simply Microscopy! The New Leica DM DigitalMicroscopes

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1 Leica DM4000 B Leica DM5000 B Simply Microscopy! The New Leica DM DigitalMicroscopes

2 Intelligence Comfort Brilliance Integration Photo courtesy of M. Okabe, Osaka University (J) This is why you entered science. It's what you work for every day. Now let the new Leica DM DigitalMicroscope help you make that brilliant discovery! Leica Microsystems AG Winner of the World s First Innovation Award: German Business Innovation Award

3 Perfection At Work - Simply Microscopy! The Exterior: New Technology in a New Design The first thing you will notice about our new DigitalMicroscopes is their new design: clear, attractive contours. Looking Through the Microscope: Fascinating Insights Once you have seen a sample through one of these new microscopes, you will never want to use any other. No microscope in this class can offer better image brilliance, field depth and contrast. Just Rely On Your Intuition Our new Leica DM DigitalMicroscope series provides the answers to many of our customers problems. One of the most frequently voiced requests was to lighten the workload. So we found ways of doing a lot of the work for you. You can operate the microscope intuitively and easily automate complex routines to suit your specific needs. Experts Call it Ergonomics. We Call it User-Friendly Ergonomics is a word often used. On our new microscopes you can actually feel it. Cooperating closely with the Fraunhofer Institute*, our designers have not only outperformed the latest technological standards but also all the ergonomic specifications. Our Product Range Caters To All Your Needs Whatever you need for your examinations or research, we can supply it: the microscopes, the cameras and even the software for analyzing and archiving your images. New All-Embracing Software Parallel to the new Leica DM DigitalMicroscope series we are offering our own totally new software concept. All our hardware and software components are now controlled from one single interface. *The Fraunhofer Institute IAO (Stuttgart, Germany) investigates ergonomic aspects of various products. In cooperation with their industrial partners they develop industrial designs to suit highest ergonomic demands. Leica DM4000 B with the basic BT25+ tube in a configuration for fluorescence and the new fully motorized phase contrast condenser 3

4 Your Benefits Intelligence Automatic Diaphragm Adjustment Our new DigitalMicroscopes automatically recognize the contrasting technique and objective that is currently in use. There is no need to adjust any more diaphragms either in transmitted or in incident light. Automatic Light Adjustment The light intensity is automatically set to the light-gathering power of the objectives. This means that the brightness of the specimen image remains constant when you switch to a different objective and there is no danger of glare. And because every task has its own specific requirements, you can adjust the light intensity individually. An impression of clarity wherever you look: Customer-specific illumination and diaphragm adjustments can be made on the front left side of the microscope. New: Transmitted Light Axis With Constant Color Intensity Control (CCIC) Too dark for viewing too bright for your digital camera: in the past it was necessary to use a white balance every time the lamp voltage was changed. The new transmitted light axis works with a color-neutral brightness control which automatically maintains a constant color temperature. So you no longer need to use neutral density filters to compensate for changes in light intensity. New Condensers for a New Level of Automation Our fully automated condensers will meet the most demanding requirements. The motorized tops, which automatically swing in and out of the light path, are applicable from 1.25x to 100x. The motorized condenser turret accommodates up to four interference prisms and up to four phase rings as well as the brightfield and darkfield positions. We also offer an integrated, motorized polarizer for fully automatic interference contrast. Clarity Wherever You Look All the settings of the Leica DM4000 B can be seen at a glance in the clearly laid out display. And the Leica DM5000 B offers you never before realized control possibilities with the completely new touch-sensitive screen. You can control all the automated features of the microscope via the touch screen there is no need to know anything about programming. The new LeicaScreen on the DM5000 B with touch-sensitive LCD display. The clearly designed menu structure uses internationally familiar pictograms instead of language for intuitive operation. 4

5 Our DigitalMicroscopes Adapt to You in Every Way The new adaptable tube can be perfectly matched to your body size and posture. You can reach the focus knobs with your hands resting on the table. The new stage allows simultaneous focus and movement control. So whatever you are examining, you are completely relaxed even if you sit at the microscope for hours at a time. Comfort New Stages and Specimen Holders For Your Convenience We have designed the new stages to satisfy the most demanding applications: scratch-resistant thanks to the ceramic coating and featuring telescopic stage drives with individually adjustable torque. To eliminate the risk of injury, we decided not to implement a rack for the y movement. The rotatable stages are suitable for one to two specimens and are available in a version for left-handed operation on request. Variable Function Keys You can assign any function you like to the six new function keys. Due to their convenient position behind the focus wheels, frequently used functions are always within easy reach. We had your comfort in mind. Five New Viewing Tubes for Pin-Sharp Images To match our new DigitalMicroscopes, we have devised a viewing tube series that will meet the highest requirements. Our new documentation tubes (which can be motorized on request*) have three switching positions and are fitted with either one or two camera ports. The new adaptable tube can be optimally adjusted to your needs. And of course, you will also find an ergonomy tube with documentation port in our product range. The viewing angle and height of the new AET22 adaptable tube can be individually adjusted to your sitting posture and body size. Plus you can vary the length of the eyepieces to suit the position of your arms. Brilliance New 1.25x Scanning Objective The new 1.25x panoramic objective is especially intended for biological sciences. In combination with our new transmitted light axis, it gives you excellent field depth and optimum illumination homogeneity for low magnification imaging. * The motorized switchable tube MBDT25+ will be available from October Together with the new condenser, our new transmitted light axis permits Koehler illumination from 1.25x - 100x. And the new 1.25x panorama objective offers excellent depth of field and perfectly homogeneous illumination. 5

6 Your Applications Our Solutions Transmitted Light Brightfield It has never been so easy to use a microscope: just put a specimen on the stage and focus! The Leica DigitalMicroscope recognizes the objective you have chosen, automatically sets the condenser top, accurately opens and closes the aperture and field diaphragms and adapts the light intensity. The automated colorneutral brightness control also filters red and orange components out of the light, giving you a constant impression of your specimen. Phase Contrast There is no further need to search and select phase contrast rings. The DM knows the right ring for the objective you have selected. Each objective has its own ring, saving you the nuisance of re-centering. Cross section through tilia wood. brightfield Interference Contrast The Leica DM5000 B is the first microscope to offer totally automated interference contrast. All the modules are fully motorized: analyzer, polarizer, condenser prisms and objective prisms. The Leica DM5000 B also knows the right condenser and objective prisms for the objective you have chosen and moves them and the polarizer and analyzer automatically into the light path. The microscope stores the fine objective prism settings for each objective so that they can be reproduced whenever needed. Darkfield and Polarization Of course, all the other contrasting techniques implemented in the Leica DM4000 B and Leica DM5000 B, such as darkfield and polarization, are automated, too. Here again, the microscope knows all the necessary components. BPAE cells with mouse anti-α-tubulin, BODIPYL FL goat anti-mouse IgG, Texas Red X phalloidin, DAPI. Molecular Probes. 6

7 Fluorescence New: FIM Technique (Fluorescence Intensity Management) This facility for reducing the fluorescence illumination in five steps minimizes the problem of fading samples. It also lets you store a separate FIM step for each fluorescence filter to obtain a constantly bright image of multiple-stained specimens. More Light With a Simple Hand Movement If you need more light, simply switch on the new booster lens, which immediately directs more light to your sample if required. 5- and 8-position Filter Cube Changer The stands of the new Leica DigitalMicroscopes are fitted with a turret for up to five fluorescence filter cubes. The DM5000 B can also be supplied with an 8-position filter turret on request. Both filters take the same type of filter cube, allowing you to switch conveniently between different versions. You have the choice between continuous switching of all cubes or direct selection of individual filter cubes. Cross section through the axis of a helianthus shoot, openly collateral bundles, phase contrast Integrated BG38 Filter Manual insertion of a BG38 filter for certain fluorescence applications is no longer necessary. The function of this filter has been integrated on the fluorescence filter cubes. Single Keystroke Contrast Switching - Easier Than Ever Before The method of changing contrasting techniques is unique. One press of the innovative function keys and the microscope switches between brightfield, phase contrast, polarization, darkfield or fluorescence automatically. In the case of the Leica DM5000 B we have even assigned all the interference contrast settings to one single key. Leica Design by Christophe Apothéloz Transgenic C. elegans displaying GFP expression. Courtesy of Dr. M. Morcos, Heidelberg, Germany 7

8 Your System Solutions The Choice Is Yours. Now and in the Future. To go with the new DigitalMicroscopes we offer you a totally new software concept which allows you to upgrade your system at any time. All future software and hardware components of Leica will be controlled from the same interface. Individual Microscope Configuration and Control The user interface is extremely easy to use. Function keys, contrasting techniques and other microscope parameters are easily configured on the computer in accordance with your preferences or the needs of your working environment. Operator errors are practically impossible, which is particularly important in a routine environment. You can also use the software to remote-control your microscope. Cameras For Every Requirement The Leica DC range of digital cameras offers the right camera for every requirement. From general to specific, our cameras are ideal: color or gray-level pictures of fluorescence specimens or industrial materials. There are cameras with a live image or real time video mode. The camera chips feature up to 12 megapixel image resolution. And with exposure times between a few µs and several minutes, every customer will find the camera for his or her application. Metaphase-spread FISH stained chromosomes Photos: Dr. Yumiko Suto, Tokyo University Courtesy of Dr Yumiko Suto, Biological Sciences, University of Tokyo, Japan. 8

9 Perfect Image Archiving and Analysis With the Leica IM1000 archiving program you can create picture galleries, annotate images and store microscope parameters. The Leica FW4000 fluorescence system offers a comprehensive solution for recording, processing, publishing and archiving images. In cooperation with other laboratories, a user-friendly interface has been designed which can be precisely tailored to specific customer requirements. The Leica CW4000 cytogenics program offers solutions for karyotyping, FISH (Fluorescence In-Situ Hybridization), MFISH (Multi-Colored FISH) and CGH (Comparative Genome Hybridization), etc. Integration 9

10 0.5x System Overview Leica DM4000 B/DM4000 M/ DM5000 B Tube program L Camera systems Leica SCR camera system Leica MPS30/60 see brochure focusing telescope focusing telescope Control panel MPS30 Film magazine 24 x 36 Motor adapter (24 x 36) without optics Camera objective 0,32x Eyepieces HC Photo 8x, 10x, 12,5x MPS30 Shutter unit with sensor MPS60 Eyepiece adapter HC Photo 8x, 10x, 12,5x graticules (for material numbers see modular system brochure) Focusing magnifier for MPS60 Control panel MPS60 Keyboard for databack C-mount 1/2 1x B-mount 1/2 Sony 1x B-mount 2/3 Sony F-mount 1/ F-mount 2/3 1.25x 1x 1.25x 0,35x 0,5x 0,63x 1x Tube top incl. 1 camera port 0,33-1,6x c-mount adapters HC, (for material numbers see mod. system brochure) c-mount HC 0,5-2,4x ENG(B) mount HC Tube top incl. 2 camera ports TV Systems Digital cameras, see brochure Leica DC150 Leica DC300 (F) Leica DC350 (F) Leica DC480 (F) Leica DC500 Monitor MFK DM MFK TV camera without optics Image analysis/ image storage Lamp housing LH 107/2 12 V 100 W Halogen lamp AET22 advanced ergo tube incl. tilting and extendable eyepieces EDT22 F 50/50 Ergo documentaion tube incl. tilting eyepieces BT25 + Basic tube BDT25 + V 100/50/0 Basic documentation tube with variable beam splitting * MBDT25 + V 100/50/0 Basic documentation tube with variable beam splitting, mot Lamp housing LH V 100 W Halogen lamp 0,55 m cable Lamp housing LH V 100 W Halogen lamp 2 mcable Lamp housing LH 106z 12 V 100 W Halogen lamp 4-lens collector 0,55 m cable Lamp housing LH 106z 12 V 100 W Halogen lamp 4-lens collector 2 mcable Intermediate piece with filter holder for 2-4 filters (D= 50 mm) mot. comparison bridge Ergomodule * Top cover incl. Magnification changer for industry (1x, 1.5x, 2x), coded tracing device Top cover discussion unit for 2 viewer discussion units for 3, 5, 10 viewers (for material numbers see modular system brochure) * incl. Magnification changer for bio/med (1x, 1.25x, 1.6x), coded Micro hardness tester MHT10 Interference attachments Michelson/Mirau 5x-50x Specimen marker Immersion cap Power unit Hg 50 W Power unit Hg 100 W Lamp housing LH 106z Hg 50 W 4-lens collector Illumination telescope Lamp housing LH 106z Hg 100 W 4-lens collector Lamp housing LH106z Hg 100 W 6-lens collector Mirror housing switchable for 2 lamp housings Stand top DM4000 M incl. Reflected light axis, incl. 4-fold Reflector turret, mot., inkl. Objective revolving nosepiece 6-fold M32, coded Cover objective revolving nosepiece Stand top DM4000 B without fluoreescence axis, incl. objective revolving nosepiece 6-folded M25, coded Compensators and IC prisms in sliders Cover objective revolving nosepiece, slot for sliders Reflector BF Reflector P. Smith Stand top DM4000 B incl. fluorescence axis, incl. 5-fold filter turret, mot., incl. 6 objective revolving nosepiece 6-fold M25, coded Reflector DF Reflector ICR Fluorescence filter systems (for material numbers see modular system brochure) Polarizer R/P Polarizer rotatable Polarizer L/ICR Analysor ICT/P Analysor 180 rotatable incident light slider Stand top DM5000 B without fluorescence axis, incl. objective revolving nosepiece 7-fold M25, coded Filter D= 25 mm various excitation manager Booster-Lens for fluorescence axis D= 25 mm Cover objective revolving nosepiece Stand top DM5000 B incl. fluorescence axis, incl. 5-fold filter turret, mot., incl. objective revolving nosepiece 7-fold M25, coded * Stand top DM5000 B incl. fluorescence axis, incl. 8-fold filter turret, mot., incl. objective revolving nosepiece 7-fold M25, coded Cover objective revolving nosepiece incl. DIC prism turret 4 pos., mot. ICT objektive prisms (for material numbers see modular system brochure) Fluorescence filter systems (for material numbers see modular system brochure) Analyser Filter system POL/IGS Immersion oil 20 ml Immersion oil 250 ml Immersion oil, low fluorescence, 10 ml Objective series BF Objective series DF Basicstand DM4000 M without TL axis, incl. LED display Incident light object guide for large specimen Tilting object guide Object holder metall Basis stand DM4000 B/DM4000 M incl. TL axis, incl. LED display x/y stage ERGO incl. stage bracket and condenser holder, fixed, 1 specimen x/y stage BIO I, incl. stage bracket and condenser holder, rotatable, 2 specimen Object holder ERGO, single-hand-operation, 1 specimen Object holder BIO I, single-hand-operation, 1 specimen Supply unit CTR Basic stand DM5000 B incl. TL axis, incl. Leica-Screen x/y stage ERGO incl. stage bracket and condenser holder, fixed, 1 specimen x/y stage BIO I, incl. stage bracket and condenser holder, rotatable, 2 specimen Object holder ERGO, single-hand-operation, 1 specimen Object holder BIO I, single-hand-operation, 1 specimen Ground plate without filter magazine mech. Focus drive x/y stage IND, incl. stage bracket and condenser holder, rotatable, 76 x 50 mm Incident light stage with reverse stage bracket for large specimen x/y stage 4" x 4" Lamp housing 12 V 100 W Halogen lamp Ground plate without filter magazine Ground plate incl. Filter magazine for 2 filters (D= 32 mm) mech. Focus drive x/y stage BIO II, oil immersion, 2 specimen Condenser BF for mot. condenser top Condenser PH (usable for BF, PH, DF, ICT), for mot. Condenser top, incl. 7-fold condenser turret, mot Condenser DIC (usable for BF, PH, DF, ICT) for mot. Condenser top, incl. 7-fold condenser turret, mot., incl. mot polarizer Object holder BIO II, oil immersion, 2 specimen Lamp housing 12V 100W Halogen lamp Ground plate without filter magazine Ground plate incl. Filter magazine for 2 filters (D= 32 mm) mech. Focus drive x/y stage BIO II, oil immersion, 2 specimen Condenser BF for mot. condenser top Condenser PH (usable for BF, PH, DF, ICT), for mot. Condenser top, incl. 7-fold condenser turret, mot Condenser DIC (usable for BF, PH, DF, ICT) for mot. Condenser top, incl. 7-fold condenser turret, mot., incl. mot polarizer Object holder BIO II, oil immersion, 2 specimen Condenser top 0.90 S Condenser top P 1.40 Oil S1 ICT condenser prisms (K1-K15) (for material numbers see modular system brochure) Set light rings DF, PH 1/1/2/ Hand press metall specimen slides Condenser DF bottom part Polarizer ICT/P Condenser DF bottom part Polarizer ICT/P Condenser top 0.50 S15 *available October DF condenser top D=

11 Technical Data DM4000 B DM5000 B Stand Power supply Integrated in stand In CTR5000 electronics box Display Information display (3.7x7.7 cm) Touch Screen (7.3x7.3 cm) with information and operation fields Transmitted light axis Illumination 12V 100W halogen lamp 12V 100W halogen lamp Automation Contrast and Light Manager (adjustment of light intensity, field and aperture diaphragm) Constant Color Intensity Control (CCIC) Contrast techniques BF (brightfield) BF (brightfield) PH (phase contrast) PH (phase contrast) DF (darkfield) DF (darkfield) POL (polarisation) POL (polarisation) ICT (interference contrast for transmitted light) Fluorescence axis Illumination 100W Hg lamp; 50W Hg lamp 100W Hg lamp; 50W Hg lamp Automation Contrast and Light Manager (adjustment of light intensity and field diaphragms) FIM (fluorescence intensity management) technique for reducing the light intensity in 5 steps Booster lens for increasing the light intensity (optional) Circular and rectangular field diaphragms for eyepiece or camera viewing Mot. filter turret 5-position 5-position 8-position Condensers Automation Mot. condenser top Mot. condenser top Mot. condenser turret (7 pos.) optional Mot. condenser turret (7 pos.) optional Mot. polariser optional Objective nosepiece 6-position M25, coded 7-position M25, coded Stages Ceramic-coated Ceramic-coated Without rack on y drive Without rack on y drive Adjustable torque Adjustable torque Telescopic stage drive Telescopic stage drive With and without 110 rotation With and without 110 rotation Left-hand version on request Left-hand version on request

12 Labelling molecules was as hard as writing on grains of dust with a pencil until recently. In my biochemistry course about 25 years ago I used to study molecules by the diagram of biochemical paths. Today, I can watch some of these old acquaintances at work and see them wandering from one living cell to the next and influencing cellular functions. Labelling molecules is now no longer a problem using a research or confocal microscope of the Leica Fluorescence Microscopy range. Leica also offers me top-precision image analysis systems for their subsequent quantification. Prof. Dr. H.J. Tanke, Leiden University Medical Centre

13 Leica Microsystems the brand for outstanding products Leica Microsystems mission is to be the world s first-choice provider of innovative solutions to our customers needs for vision, measurement, lithography and analysis of microstructures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition. Leica Microsystems an international company with a strong network of customer services Australia: Gladesville Tel Fax Austria: Vienna Tel Fax Canada: Richmond Hill/Ontario Tel Fax Denmark: Herlev Tel Fax France: Rueil-Malmaison Tel Fax Germany: Bensheim Tel Fax Italy: Milan Tel Fax Japan: Tokyo Tel Fax Korea: Seoul Tel Fax Netherlands: Rijswijk Tel Fax People s Rep. of China: Hong Kong Tel Fax Portugal: Lisbon Tel Fax Singapore: Tel Fax Spain: Barcelona Tel Fax Sweden: Sollentuna Tel Fax Switzerland: Glattbrugg Tel Fax United Kingdom: Milton Keynes Tel Fax USA: Bannockburn/lllinois Tel Fax and representatives of Leica Microsystems in more than 100 countries. The companies of the Leica Microsystems Group operate internationally in five business segments, where we rank with the market leaders. Microscopy Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. Specimen Preparation We specialize in supplying complete solutions for histology and cytopathology. Imaging Systems With confocal laser technology and image analysis systems, we provide three-dimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences. Medical Equipment Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery. With automated instruments for ophthalmology, we enable new diagnostic methods to be applied. Semiconductor Equipment Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world. Copyright Leica Microsystems Wetzlar GmbH Ernst-Leitz-Strasse Wetzlar Germany 2002 Tel. ( ) 29-0 Fax ( ) LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. Order nos. of the editions in: English German French Spanish Italian Part-No Printed on chlorine-free bleached paper.