Leica DMI 3000 M Leica DMI 5000 M. Modular system Stands, modules, accessories

Transcription

1 Leica DMI 3000 M Leica DMI 5000 M Modular system Stands, modules, accessories

2 Leica DMI 3000 M/DMI 5000 M Modular system Issued: August 2008 Contents Leica DMI Series Basic stands page 3 System overview page 4 Camera ports page 11 Observation and documentation tubes page 12 Eyepieces page 12 Mot. reflector revolver / Mot. magnification changer page 15 Stages and sample holders page 17 Incident light illumination page 19 Condensers and accessories page 29 Light sources, lamp housing, supply units page 31 TV adapter page 34 Objectives, objective turret, optics page 35 Accessories page 36 System overview page 38 Published August 2008 Leica Microsystems CMS GmbH Ernst-Leitz-Straße D Wetzlar (Germany) Responsible for contents: Stefan Motyka (Marketing Industry Division, Product Management) Holger Grasse (Safety Officer according to MPG 30) In case of technical questions, please contact the hotline: Tel. +49(0) Fax +49(0) MQM-Hotline@leica-microsystems.com Or for questions related to the software: DI-support@leica-microsystems.com 2

3 Leica DMI Series Basic stands The Leica DMI Series is the rigorous further development of the proven inverted research microscopes for use in industrial applications. Applications range from routine tasks to research tasks, quality inspections, material analyses for the development of new materials or in quality inspection. All contrasting methods such as bright field, dark field, DIC, POL or fluorescence are an integrated component of the microscope and are quickly and easily adapted or changed. Variable illumination and imaging beam paths as well as HCS optics, modular accessories and an extensive peripheral program complement the inverted research stand Leica DMI 5000 M. Fig. 1: Right side of stand DMI 3000 M Basic stand The basic stand is the solid core of the microscope. It contains focusing, objective turret, stage mount, mounts for incident light or transmitted light illumination units. In addition the DMI 5000 M features a LCD monitor for status display of the microscope as well as various function keys for motorized functions, such as changing magnification, switching camera ports or reflector splitter for the different contrasting methods. Interfaces for PCs as well as internal and external peripheral devices are located on the back of the basic stand. The trendsetting modular concept is characterized by the individual selection of modules, such as light sources, objectives, eyepieces, tubes, stages, filters as well as incident light and transmitted light components. This allows every user to combine his or her personal application-orientated microscope system. Fig. 2: Front view of stand DMI 5000 M The exploded view (see page 38) provides an overview of this modular system. With their PC-based "e-leica" configuration system, our representatives can provide you with effective support in putting together the optimal system for your requirements. In addition, our applications specialists in Wetzlar can help you with specific questions. Thanks to its modular design principle, you can modify and/or extend your system to suit your requirements at any time. Fig. 3: Right side of stand DMI 5000 M Ergonomic considerations were given a high priority in all stand variants, which particularly applies to important controls that are always designed for easy reach and operation. The massive, functional and rugged design of the stands ensures ease of use and image stability over the course of the application and right up to the highest magnification. Optics of the highest standards allows for brilliant images with high contrast and resolution for any conceivable application. Fig. 4: Left side of stand DMI 5000 M 3

4 Leica DMI 5000 M 1. Basic stands / tubes p. 6, 7 2. Focusing drive p Objective turret p Stages p Reflectors p Lamp housings p Ports p. 9 4

5 Filter p Illumination manager p Contrast manager p DIC p Magnification changer (in the stand) p. 6, Status display p Magnification changer (operation) p Camera port switch-over p Method changeover p. 7 5

6 A Leica DMI Microscope stand is defined as a combination of 11888xxx article numbers such as: microscope body optic carrier camera ports incident light module and magnification changers transmitted light arms front modules etc. Those xxx article numbers always come as a integral part of the aligned complete system. These articles are not possible to order as an individual component. 3 basic stands are available for the Leica DMI M Series Leica DMI 3000 M Manual inverted stand Leica DMI 5000 M Inverted stand with manual focus drive Leica DMI 5000 M Inverted stand with motorized focus drive 265mm Dimensions and Weights 126mm 268mm 151mm Weights: (without CTR Box Leica DMI series incident light configuration, complete with lamp housing and tube approx. 19 kg Technical Data mm 665mm Operating voltage: V Frequency: 50/60 Hz Power consumption: P max: 180 W Operating temperature: 10 C 36 C Relative humidity: 0 80% at 30 C 633mm 6

7 All basic stands are ready for: Incident-light examination in: Bright field Dark field Polarization contrast Differential interference contrast (ICR) Fluorescence Transmitted-light examination in: Bright field Dark field Polarization contrast Differential interference contrast (ICT) Phase contrast Fig. 5: Tube with top port Objective turret Manual for Leica DMI 3000 M Motorized and coded for Leica DMI 5000 M 5-fold for objectives with M32 thread and 45 mm parfocal distance For DIC, motorized (manual for DMI 3000 M) / coded Wollaston prism carousel Z focus (focus hand wheels on both sides) Manual for Leica DMI 3000 M Manual for Leica DMI 5000 M Motorized and coded for Leica DMI 5000 M 9 mm travel (1 mm below, 8 mm above the stage) Leica DMI 5000 M only 5 focus steps: 0.05 µm; 0.1 µm; 0.7 µm; 1.5 µm; 5.0 µm Electronic focus repositioning Automatic lowering prior to objective change Electronic parfocality Note: Control elements see page 8 The Camera Ports All Leica DMI 3000 M are ready for: manual left camera port (80%) optional manual top port (Fig. 5 p. 7) with 100/0% and 0/100% eyepiece/port split or with 100/0% and 50/50% eyepiece/port split All DMI 5000 M stands are ready for: motorized right camera port with (100%) and/or (80%) and/or (50%) motorized left camera port with (100%) and/or (80%) and/or (50%) motorized right and left camera port with (100%) and/or (80%) and/or (50%) manual top port with 100/0% and 50/50% eyepiece/port split 7

8 Magnification changer All DMI 5000 M stands are ready for: A motorized magnification changer with 1 3 different, freelyconfigurable magnification levels affecting all camera ports: 1.0x (always available) 1.5x 1.6x 2.0x Fig. 6: Controls (left) DMI5000 M Fig. 7: Controls (right) DMI5000 M Alternatively, manual for DMI 3000 M All basic stands are ready for a manual magnification changer with two positions. This magnification changer affects only the top port and eyepieces: 1.0x (always available) 1.5x 2.0x Electronical control elements (not for DMI 3000 M) (Fig. 6, 7, 8 p. 8) 7 fixed control buttons for illumination and apertures 7 variable function buttons behind the focus controls 3 fixed control buttons for focus thresholds (for DMI 5000 M stand with motorized focus) 2 focus hand wheels 5 buttons for reflector cube and shutter 4 buttons for motorized magnification changers and ports SmartMove: ergonomic controller for x, y, z and 4 additional variable function buttons (Fig. 15 p. 10) Leica STP6000: external controller for x, y, z and 11 additional variable function buttons (Fig. 16 p. 10) Interfaces (not for DMI 3000 M) (Fig. 9 p. 8) 2 x RS232C 2 x USB 4 x external/internal peripherals Fig. 8: Controls (front)dmi5000 M Tubes (Fig. 19, 20, 21 p. 12) Fixed Bino Tube Ergonomic tube with or without camera port at left. Height and angle adjustment (30 45 ) 2 switch settings: 100% VIS and 50% VIS/50% camera or 100% VIS and 0%VIS/100% camera Eye spacing adjustment Field number: 25 mm Viewing channel Optics Leica HCS infinity optics Tube factor 1x 25 mm field of view Fig. 9: Back panel 8

9 Motorized incident-light axis for DMI 5000M (Fig. 18 p. 11) Automatic Illumination Manager (aperture, field diaphragm, intensity, contrast method switching) Automatic constant color intensity control (CCIC) Lamp housing mount for up to 3 interchangeable light sources Motorized 4x reflector revolver Manual transmitted-light axis (Fig. 61, 62 p. 27) With Condenser Quick Changer for all condensers Manual field diaphragm Manual shutter Manual filter switching Lamp housing mount for interchangeable lamp housings Automatic, electronic condenser identification Fig. 10: Viewing channel Stages (p. 17) Fixed regular stages Ceramic-coated stage plate (248 mm x 204 mm) Manual 3-plate stage (page 18) Positioning range 100 mm x 40 mm Optionally different inserts for various applications, dimensions of inserts: outside 160 mm x 110 mm (compatible with motorized stages); rated inside diameter 5 mm, 10 mm, 20 mm and 40 mm Motorized 3-plate stage (page 18) Positioning range 100 mm x 40 mm Optionally different inserts for various applications, dimensions of inserts: outside 160 mm x 110 mm (compatible with scanning stages and manual stages); rated inside diameter 5 mm, 10 mm, 20 mm and 40 mm Scanning stage IM 100 x 40 (p. 18) Both motors below stage 1 mm, 2 mm, 4 mm spindle rise (higher resolution vs. higher speed) Optionally different inserts for various applications, dimensions of inserts: outside 160 mm x 110 mm (compatible with 3- plate stages); rated inside diameter 5 mm, 10 mm, 20 mm and 40 mm Fig. 11: Transmitted-light illumination arm Fig. 12: Incident-light axis illumination 9

10 Stand variants 3 basic stands are available for the DMI 3000 M/DMI 5000 M. These basic stands differ essentially by a motorized or a manual focusing drive. DMI 3000 M basic stand with manual focus (Fig. 1 p. 3) (with manual DIC turret) DMI 5000 M basic stand with manual focus (Fig. 3 p. 3) (with manual or motorized DIC turret) DMI 5000 M basic stand with motorized focus (Fig. 4 p. 3) (with manual or motorized DIC turret) Fig. 13: Leica CTR4000 Electronics boxes (Fig. 13,14 p. 10) With built-in power supply 12 V 100 W and for control of the automatic microscope functions. With interface RS 232 for PC connection including RS 232 cable. AC power supply V, Hz Z-drive man. Z-drive mot. Stage manual Stage Tack Stage Scanning Fig. 14: Leica CTR6500 CTR CTR CTR x x x x x x x SmartMove (Fig. 15 p. 10) x/y/z-ergo control for the control of the electronic focus (z) and motorized stage (x/y). With 4 additional freely programmable function keys Leica STP6000 (Fig. 16 p. 10) External Touch Panel for electronic focus (z) and motor stage (x/y). With 11 freely programmable function keys Fig. 15: Leica SmartMove Fig. 16: Leica STP

11 Observation and Documentation Ports Camera ports for Leica DMI 3000 M The Leica DMI 3000 M can be equipped with a manual left camera port which features a mechanical slider with two positions. 1st position for 100 % light to the eyepieces. 2nd position percentage of light to the side port rest to eyepieces Two choices are available: Manual left camera side port 100/ Manual left camera side port 80/ Camera ports for Leica DMI 5000 M The DMI 5000 M can be fitted with different camera ports. The manual top port is selected together with the tube (see p. 12). Select the motorized side ports with the following modules: Motorized side port, left only In addition, up to three different prisms are selected for this variant: Side port prism, 100% left Side port prism, 80% left Side port prism, 50% left Fig. 17: Light Path Motorized side port, right only In addition, up to three different prisms are selected for this variant: Side port prism, 100% right Side port prism, 80% right Side port prism, 50% right Motorized side port, right and left In addition, up to three different prisms are selected for this variant: Side port prism, 100% right Side port prism, 100% left Side port prism, 80% right Side port prism, 80% left Side port prism, 50% right Side port prism, 50% left Fig. 18: 4x reflector revolver and motorized magnification changer No side port This variant requires the port compensation module 11

12 Observation and documentation tubes Fig. 19: Bino-ergotube The Leica DMI microscopes feature a fixed bino tube and several ergonomic tubes integrated in the stand. The interpupillary distance can be adjusted according to the Siedentopf principle. The complex tube lens system is based on an infinite beam path. It converges the parallel beam path coming from the lens and forms the object in the intermediate image plane. Also, the tube lens system, together with the eyepieces, corrects the remaining image errors not corrected by the objective. The following applies to all tubes: Siedentopf design Field of view 25 mm Eyepiece diameter 30 mm Interpupillary distance range mm Viewing angle 45 (Fix tube) Variable viewing angle (Ergo tubes) View chute to observe samples without eyepieces Binocular fix tube Binocular fix observation tube Fig. 20: Siedentopf tube with inter - pupillary distance adjustment Binocular ergonomical tube (Fig. 19 p. 11) Binocular observation tube Trinocular ergonomical tube (Fig. 21 p. 11) Binocular observation tube with manually switchable side camera port and variable light path, 100% visual/0% camera, and 0% visual and 100% camera Trinocular ergonomic tube Binocular observation tube with side camera port and variable light path, 100% visual/0% camera, and 50% visual/50% camera Fig. 21: Trino-ergotube Eyepieces for tube viewing (Fig. 22 p. 11) A wide range of eyepieces with 10x, 12.5x, 16x or 25x magnification (for different field numbers of up to 25 mm) are available for the tubes. Special eyepieces for eyeglass wearers are available, as are eyepieces with adjustable eyelenses (M eyepieces) designed to accommodate a variety of graticules. The standard eyepiece is the 10x eyepiece, microphotography recording frequently prefers the 12.5x eyepiece magnification due to the higher focusing accuracy. However, the observed object field is smaller. The 16x and 25x eyepiece magnifications are only meaningful for special cases; exceeding the "beneficial magnification" (V < 1000x objective aperture) can often be expected, i.e. possible fuzziness may occur. All eyepieces have removable or fold-down eyecups and can be used with or without eyeglasses. Eyepieces identified with M are equipped with a focusing eyelens for dioptric equalization (from 6.8 to +4.2 or 6 to +5) and graticule holder. 12

13 The outside eyepiece diameter is D = 30 mm. Graticule diameter D = 26 mm. The eyepiece data are engraved, e.g. HC PLAN 10x/20 oo M. HC PLAN = correction type, 10x = magnification/20 = field number FOV, oo = for eyeglass wearers (high exit pupil), M = dioptric adjustment/graticule holder Eyepieces with FOV 20 Eyepiece HC PLAN 10x/20 BR Eyepiece HC PLAN 10x/20 BR.M Eyepiece with FOV 22 Eyepiece HC PLAN S 10x/22 Br.M Fig. 22: Eyepieces Eyepiece with FOV 25 Eyepiece HC PLAN S 10x/25 Br.M Special eyepieces with high magnification Eyepiece HC PLAN 12.5x/16 Br.M Eyepiece 16x/14B, adjustable Eyepiece 25x/9.5B, adjustable Distance ring for eyepieces 16x/14 B and eyepiece 25x/9.5B Photo eyepieces, eyepiece tubes, focusing and framing graticules The adaptation of microphotography equipment requires an exactly matched combination of photo eyepiece, eyepiece tube and focusing and framing graticule. The focusing and framing graticule is inserted in an HC PLAN M observation eyepiece and shows the respective imaging format. In addition, the graticule features focusing marks for exact focusing. Fig. 23: Photo eyepieces For 10x photo eyepiece Eyepiece tube HC DR 27/10x for MPS Eyepiece HC 10x/16 Photo Focusing and framing graticule F6, 26 mm For 8x photo eyepiece Eyepiece tube HC DR 27/8x for MPS Eyepiece HC 8x/20 Photo Focusing and framing graticule F5, 26 mm For 12,5x photo eyepiece Eyepiece tube HC DR 27/12,5x for MPS Eyepiece HC 12.5x/13 Photo Focusing and framing graticule F8, 26 mm

14 Graticule Graticules for length measurements, comparison and counting method For HC PLAN eyepieces Graticule 10 mm = 100 parts, D = 26 mm Graticule 10 mm = 200 parts, D = 26 mm Crosshair graticule, D = 26 mm Crosshair graticule with graduation, 10 mm = 100 parts, D = 26 mm Graticule with grid 10 x 10 mm, 0.1 mm graduation, D = 26 mm Graticule with grid 10 x 10 mm, 1 mm graduation, D = 26 mm Snyder-Graff-Meth. graticule, D = 26 mm (for 10x eyepiece only) ASTME 112 graticule, D = 26 mm (for 10x eyepiece only) Graticule for steel inclusion rating (ISO 4967) Graticule for steel inclusion rating (EN10247) Stage micrometer Transmitted light 2 mm = 200T, glass carrier with scale 1 scale interval = 10 µm Incident light 10 mm = 100T for overview objectives (e.g. 1.25) Graticule Graticule Graticule Graticule Graticule Graticule Graticule Graticule Graticule

15 Motorized reflector revolver/ motorized magnification changer Up to 4 reflector filter blocks on the reflector turret contain a perfectly matched combination for all standard contrast methods in material analysis. The disk is integrated in the stand and is within the DMI5000 M stands fully motorized and coded. If the contrast method is changed, the filter blocks are switched in less than 0.2 sec. A locking button releases the disk in a drawer for easy, convenient filter replacement within seconds, without tools. 2 reflector filter blocks feature spring clamps for engaging in the incident-light revolver, filter blocks are firmly screwed into the other two positions. The optional motorized magnification changer is located on the reflector revolver. In addition to the 1x tube lens, two further magnification levels can be introduced here on an additional disk with 3 positions. This motorized magnification changer acts on the right as well as the left camera port as well as the tube and, if necessary, the top port. Fig. 24: 4x reflector revolver and motorized magnification changer Motorized 4x reflector revolver (as in Fig. 24 p. 15) with fixed 1x tube lens (no additional lenses can be inserted) with mount of up to 4 reflectors Motorized 4x reflector revolver with mot. magnification changer (Fig. 24, 25 p. 15) with mount of up to 4 reflectors with motorized magnification changer including 1x tube lens at position Magnification levels for the manual magnification changer of DMI 3000 M This magnification chnager affects the top port and the eyepieces. 1.5x tube lens for manual magnification changer x tube lens for manual magnification changer Fig. 25: 4x reflector revolver and motorized magnification changer Magnification levels for the motorized magnification changer of DMI 5000 M In addition to the 1x tube lens, one or two magnification lenses can be installed on the disk. 1.5x tube lens for motorized magnification changer x tube lens for motorized magnification changer x tube lens for motorized magnification changer

16 Leica CTR Boxes Leica CTR4000 Electronics Box (Fig. 26 p. 16) Power supply for 12 V 100 W. AC power supply V, Hz. For all stands without motor focus and without motorized stages Fig. 26: Leica CTR4000 Leica CTR5500 Electronics Box With built-in power supply 12 V 100 W and for control of the automatic microscope functions. With interface RS232 for PC connection including RS232 cable. AC power supply V, Hz. For all stands with motor focus and all stands without motorized stage (for fixed stage and manual stages only) Leica CTR6000 Electronics Box With built-in power supply 12 V 100 W and for control of the automatic microscope functions. With interface RS232 for PC connection including RS232 cable. AC power supply V, Hz. For all stands with motor focus and all stands with motorized 3-plate stages (rack) Fig. 27: Leica CTR6500 Leica CTR6500 Electronics Box (Fig. 27 p. 16) With built-in power supply 12 V 100 W and for control of the automatic microscope functions. With interface RS 232 and USB connection including cables. AC power supply V, Hz. For all stands with motor focus and all stands with a motorized scanning stage (spindle) SmartMove (Fig. 28 p. 16) x/y/z-ergo control panel for electronic focus (z) and motor stage (x/y). With 4 freely programmable function keys Fig. 28: Leica SmartMove Leica STP6000 (Fig. 29 p. 16) External Touch Panel for electronic focus (z) and motor stage (x/y). With 11 freely programmable function keys Fig. 29: Leica STP

17 Stages and sample holders A variety of stages is available for the Leica DMI 3000 M and DMI 5000 M. Depending upon the application, the following stages can be selected: Fixed stage (248 x 204 mm) Manual 3-plate stage, positioning range 100 x 40 mm Motorized 3-plate stage, positioning range 100 x 40 mm Scanning stage 100 x 40 (motors on bottom) Fixed stage (Fig. 30 p. 17) The 20-mm high stages are solidly attached to the microscope by 3 screws, an attachable object guide can be mounted either on the right or the left. Size 248 x 204 mm Aluminum, black anodized, extremely scratch resistant, precision plane-parallel. Mount options for 88-mm round inserts with openings of 5 mm, 10 mm, 20 mm and 40 mm (see inserts for manual and motorized stages, a round 88 mm insert with 10 mm opening is included). Bores on the left and right side for the adaptation of attachable object guides. 88 mm Inserts with different openings for fixed stages and 160 x 110 mm inserts for 3-plate stages and scanning stages Insert with 5 mm opening Insert with 10 mm opening* Insert with 20 mm opening Insert with 40 mm opening Fig. 30: Fixed stage Fig. 31: Attachable mechanical stage *) Insert with 10 mm opening is already included in stages Attachable object guide for fixed stages (Fig. 31 p. 17) (for ) Attachable mechanical object guide for fixed working stage to accept different application inserts. Attachable to the fixed stage either on the right or the left. Positioning range: 100 mm x 40 mm Ergonomic operating arm: low position, does not interfere with microscope controls or camera ports, with coaxial drive for x and y. Adjustable torque, extremely precise and sensitive. Wear-resistant ceramic bearing surfaces in x and y. Z positioning precision adjustable over the positioning range. Precise snap-in mechanism for a variety of inserts. Integrated scaling in x and y (optional for a number of inserts) Inserts for object guide The mounting frames are inserted into the attachable mechanical stage via a precise snap-in mechanism. Material: aluminum, black anodized. Universal mounting frame M glass slide Universal mounting frame M duo for 2 glass slides Mounting frame for glass slide 76 mm x 26 mm Fig. 32: Universal mounting frame M 17

18 Manual 3-plate-stage (without insert) (Fig. 33 p. 18) Traveling range: 100 mm x 40 mm Aluminum, extremely scratch-resistant, precisely plane-parallel for 160 x 110 mm inserts. Ergonomic operating arm: low position, does not interfere with microscope controls or camera ports, with coaxial drive for x and y. Adjustable torque, extremely precise and sensitive. Three-point mounting Fig. 33: Manual 3-plate-stage Motorized 3-plate-stage (without insert) (Fig. 34 p. 18) Traveling range: 100 mm x 40 mm Aluminum, extremely scratch-resistant, precisely plane-parallel for 160 x 110 mm inserts. Control via the Leica Application Suite software in conjunction with the SmartMove remote control (see Fig. 28 p. 16) or STP6000 (see Fig. 35 p. 18) Extremely precise and sensitive. Three-point mounting Cable adapter Using a 3-plate stage in combination with a Leica CTR6500 electronic box an adapter is necessary Fig. 34: Scanning stage 100 x 40 Fig. 35: Leica STP6000 Scanning stage 100 x 40 (without insert) (Fig. 34 p. 18) 3-plate scanning stage without insert Traveling range: 100 mm x 40 mm Three-point mounting. Aluminum, extremely scratch-resistant, precisely plane-parallel for 160 x 110 mm inserts. Max. positioning speed: 10 mm/sec. to 100 mm/sec.* Max. resolution: µm* Reproducibility: <1 µm* Precision: ± 3 µm* *) Depending upon built-in spindle: 1 mm, 2 mm or 4 mm With motors on bottom Prerequisites for scanning stages Leica SmartMove for DM/DMI series or Leica STP Leica CTR6500 electronics box incl. cable for scanning stage Inserts for manual and motorized stages (Fig. 36 p. 18) 3-plate stages and scanning stages are ready to accept inserts with outside dimensions of 160 x 110 mm. Depending upon the specimen size, the following inserts can be selected: Mounting frame for round inserts (160 x 110 mm) Fig. 36: Mounting frame (160 x 110 mm) with insert 18 Insert with 5 mm inside diameter Insert with 10 mm inside diameter Insert with 20 mm inside diameter Insert with 40 mm inside diameter Universal retaining frame LK for 1 glass slide Universal retaining frame LK duo for 2 glass slides

19 Incident light illumination The Leica DMI M series is a system microscope for the following incident-light methods in materials testing and materials research: Bright field, dark field, polarization contrast, interference contrast (ICR) and fluorescence. In addition oblique illumination is integrated in the DMI 3000 M. With the Leica DMI 5000 M routine functions such as the adjustment of intensity and incident-light diaphragms to the objective magnification and specimen conditions are automated and offer optimal working comfort. The incident-light axis of the Leica DMI M series is equipped with a completely new incident-light system. Optimal light flux with the greatest intensity and homogeneity is generated. The field and aperture diaphragms can be centered and allow for an optimal Köhler illumination. The result is the best possible resolution, contrast and depth of field. Different lamp mounts and mirror housings (up to 3 ports) even allow for illumination from several light sources. A lamp housing can be used for the incident-light contrast methods bright field, dark field, polarization contrast and interference contrast while another lamp housing can be adapted for fluorescence. The regular incident fluorescence illumination is based on highintensity high-pressure 100 W mercury burners (Hg 100 W) with emission in the short-wave spectral range. Furthermore xenon lamps (XE 75 W) are used when a broadband spectrum range is to be covered. Up to 4 reflector filter blocks on the reflector turret contain a perfectly matched combination for all standard contrast methods. The disk is integrated in the stand and is fully motorized and coded. The Leica DMI 3000 M is equipped with a manual reflector turret. If the contrast method is changed, the filter blocks are switched in less than 0.2 sec. A locking button releases the disk in a drawer for easy, convenient filter replacement within seconds, without tools. 2 reflector filter blocks feature spring clamps for engaging in the incident-light revolver, filter blocks are firmly screwed into the other two positions. The filter blocks used for fluorescence are designed to guarantee zero pixel shift and completely suppress stray light to ensure a completely black background for the fluorescent image. A laterally attached adjustment window together with the supplied adjustment tool allows for a perfect setting of the lamp. Fig. 37: Filter block mount Fig. 38: Opening the drawer Abb. 39: Exchange filter cubes DMI 3000 M 19

20 Reflectors, filters, incident-light polarizers and analyzers Fig. 40: Bright-field reflector (BF) Fig. 41: P reflector to Smith The incident light illuminator is based on a 12 V, 100 W halogen bulb whose luminous intensity is automatically adjusted after presetting to the reflection of the specimen and the light flow in the respective objective. Caution: Adaptation with incident light of the DMI 5000 M is colorneutral: The constant color intensity control (CCIC) integrated in the incident-light axis of the DMI 5000 M allows for light adaptation without the interfering reddish image backgrounds of conventional systems. Up to 4 reflectors of size k can be used in the reflector turret of the Leica DMI M Series. The following filter blocks can be selected for the respective contrast methods: Incident-light bright field With the push of a button on a DMI 5000 M, the BF reflector or the Smith reflector are rotated into the beam path. The motorized coded objective turret recognizes the objective and automatically adjusts luminous intensity, incident light aperture and field diaphragm to the preset values. The user can adjust and overwrite the preset values at any time. Objective magnifications 1.25x 150x (1.25x and 1.6x with glint-protection device). All incident-light functions of a DMI 3000 M are carried out fully manual. Two different bright field reflectors are available that differ according to the adaptation in the reflector disk: BF reflector, fixed (without illustration) Bright-field reflector that is screwed into the reflector disk, already adapted at the factory. 45 neutral glass insert divider for incident-light bright field, ICR and pol Optional: BF reflector, variable Bright-field reflector that can be inserted in one of the two variable positions of the reflector disk. 45 neutral glass insert divider for incident-light bright field, ICR and pol Optional: Gray filter N16 Insertable on BF reflector for light reduction when working with external light sources Optional: P reflector to Smith (Fig. 41 p. 20) Firmly adapted at the factory, mirror and glass insert divider 2 x 22.5 with lens for incident-light bright field, ICR and POL contrast. High degree of polarization, especially well suited for ICR and polarizing incident light

21 Incident-light dark field With a push of the button of the Leica DMI 5000 M, the DF reflector is rotated into the beam path. The motorized coded objective turret recognizes the objective and automatically adjusts intensity, incident light aperture and field diaphragm to the light values required for dark field. The user can adjust and overwrite the preset values at any time. Using the DMI 3000 M the DF reflector is rotated manually into the beam path. Objective magnifications 5x 150x. Required: DF reflector, fixed (Fig. 42 p. 21) Adapted at the factory, 45 ring mirror for incident-light dark field Fig. 42: Dark-field reflector (DF) Incident-light light filter D = 25 mm Two of the incident-light filters listed below are placed in the IL filter slide (Fig. 43 a p. 21). The complete filter slide is located in the filter slot in the rear section of the stand. IL filter slide (Fig. 44 p. 21) to mount the above light filters D = 25 mm The following incident-light filters can be selected: Panchromatic green filter For B&W photography and sensitivity increase of the eye a Fig. 43 a: Position of incident-light filter slide Blue glass filter BG20 Color contrast filter for contrast increase Daylight filter DLF Blue, conversion filter for daylight film and visual observation Interference green filter VSS 546 nm For monochromatic light in interferometry Fig. 44: IL filter slide 21

22 Incident-light interference contrast (ICR) The manual incident-light interference contrast requires a (rotating) polarizer, an analyzer and a set of Wollaston prisms. In addition, the Leica DMI 5000 M offers a fully automated ICR function. Fig. 45: DIC disk and POL slides under objective turret Fig. 46: Motorized DIC objective prism disk The Leica ICR demonstrates its strengths during the analysis of defects, for example during the examination of surface structures. Objectives with magnifications from 20x to 100x can be used for ICR. Fast (automatic) switching between ICR, bright field and dark field is possible at all times without the need for DIC prisms to remain in the beam path. The IC prisms at the objective side are located in the objective prism disk (Fig. 46). For valid combinations of prisms and objectives, please refer to the objective list The coded objective turret of the DMI 5000 M knows the objective. The motorized objective prism disk selects the correct objective prism and sets the bias. The reflector revolver fully automatically moves the ICR reflector into the beam path. In addition, light intensity, aperture diaphragm and field diaphragm are automatically adjusted to the required values. The user can adjust and overwrite the preset values at any time. If a BF or Smith reflector is used instead of the IRC reflector, the corresponding IC prism must be manually rotated into the beam path and adjusted. When the manual objective system prism disk is used, the Leica display of the DMI 5000 M shows the information for the required prisms. Manual coded DIC objective system prism disk Motorized coded DIC objective system prism disk (not for DMI 3000 M) a Fig. 47 a: Position of analyzer slider The required incident-light polarizer and analyzer elements can be supplied on a slider and as an enclosed ICR filter system. ICT/ICR objective prisms (for assembly in DIC disk or ) IC objective prism A IC objective prism B IC objective prism B IC objective prism C IC objective prism C IC objective prism C IC objective prism D IC objective prism D IC objective prism E Fig. 48: Objective prism D 22

23 Condenser prisms (Ø 32mm) for DIC dark field IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K IC condenser prism K12, oil IC condenser prism K ICR equipment with polarization slider: The polarization mount is located on the left side of the stand below the objective turret as shown in Fig. 45. The cover for the objective DIC slider opening is a component of the stand. Fig. 49: R/ICR polarizer Fig. 50: L/ICR polarizer R/ICR polarizer (Fig. 49 p. 23) In slider 29 x 11.5 mm. Fixed orientation 90 (north-south) with MgF2 plate for homogenizing the brightness across the field. Interference contrast is possible only via DIC prism (vignetting is possible) L/ICR polarizer (Fig. 50 p. 23) In slider 29 x 11.5 mm. Fixed orientation 0 (east-west) with Lambda plate. 180 rotation for activation or deactivation of the Lambda plate for color contrast Fig. 51: Analyzer, 180 The analyzer slider is located on the right side of the stand below the objective turret as shown in Fig. 52. The cover for the objective slider opening is a component of the stand. Analyzer, 180 (Fig. 51 p. 23) In slider 30 x 5 mm, rotating 180 with drum head graduation of 5, 1 scale line = Fig. 52: Analyzer ICT/P Analyzer, ICT/P (Fig. 52 p. 23) In slide bar 30 x 5 mm, fixed orientation Alternative: Filter system for fully automated ICR (Fig. 53 p. 23) For quick and convenient operation. Neutral splitter with permanently installed and fixedly crossed polarizer and analyzer and MgF2 plate for homogenizing the brightness over the field. For use in the reflector disk. Interference color contrast is possible only via DIC prism (vignetting is possible) Fig. 53: ICR filter block with firmly crossed polarizer and analyzer 23

24 Incident-light polarization contrast A fixed or revolving polarizer and an analyzer are required for incident-light polarization contrast. Low-stress objectives and condenser lenses are a requirement for optimal quality of the polarization contrast. Instead of polarizer and analyzer as single slider, it is also possible to use complete polarization filter systems if the examination does not require a revolving polarizer. They consist of a neutral divider with firmly crossed polarizers. Fig. 54: P reflector to Smith P reflector to Smith (Fig. 54 p. 24) Firmly adapted at the factory, mirror and glass insert divider 2 x 22.5 with lens for incident-light bright field, ICR and polarizer contrast High degree of polarization, especially well suited for ICR and polarizing incident light Pol. filter system, IGS (Fig. 55 p. 24) Reflector (size k) consisting of polarizer and analyzer in firmly crossed position R/P polarizer (Fig. 56 p. 24) In slider 29 x 11.5 mm. Changeable in 3 click stops 0 (east-west), 45 (diagonal), 90 (north-south) Fig. 55: Pol. filter system, IGS Alternative: Polarizer, revolving (Fig. 57 p. 24) In slider 29 x 11.5 mm. Polarizer revolution 90, Lambda plate revolution approx. 14. For color contrasting of anisotropic material surfaces, e.g. aluminum (sensitive tint method) Fig. 56: R/P polarizer Fig. 57: Polarizer, revolving 24

25 Michelson/Mirau incident-light interference device Surface analyses with accuracies in the range of a few nanometers are performed at the DMI 5000 M with the Michelson/Mirau mount. The exact and quick measurement of filters in the optical industry or the analysis of polished surfaces are application areas of this non-destructive analysis system. This requires the following: 5x, Michelson consisting of: - PL FLUOTAR 5x/0.15 Michelson objective - 5x Michelson dual-beam mount - Base structure Adapter ring M32/ Fig. 58: Interference base component 10x, Mirau consisting of: - PL FLUOTAR 10x/0.30 Mirau objective - 10x Mirau dual-beam mount - Base structure Adapter ring M32/ x, Mirau consisting of: - N Plan H 20x/0.40 Mirau objective - 20x Mirau dual-beam mount - Base structure Adapter ring M32/ x, Mirau consisting of: - N Plan H 50x/0.50 Mirau objective - 50x Mirau dual-beam mount - Base structure Adapter ring M32/ Interference green filter VSS 546 (25 mm Ø) for monochromatic light, without mount IL filter holder for Ø 25 mm filter

26 Fig. 59: Fluorescence filter system A4 Incident-light fluorescence The incident-light fluorescence of the DMI 3000 M/DMI 5000 M is equipped with a completely new incident-light system. Different lamp housings and mirror housings (up to 3 ports) that can be adapted to DM 3000 M/DM I 5000 M also allow for simultaneous illumination with several light sources (lamp housings see p. 31). Optimal light flux with the greatest intensity and homogeneity is guaranteed. Light traps and the suppression of natural fluorescence result in an optimal black background. The regular incident fluorescence illumination is based on high-intensity highpressure 100 W mercury burners (Hg 100 W) with emission in the short-wave spectral range. Furthermore, xenon lamps (XE 75 W) are used when a broadband spectrum range is to be covered. The incident-light axis of the Leica DMI 5000 M is equipped with motorized light stop which is activated with the push of a button or via software to prevent specimens from bleaching. 2 additional fluorescence filter blocks on the reflector turret can be used in addition to the reflector cubes described on page 16. A locking button releases the disk in a drawer for easy, convenient filter replacement within seconds, without tools. Switching filter blocks is done in less than 0.2 sec. The filter systems (blocks) feature spring clamps for engaging in the incident-light revolver. All blocks are designed so that they guarantee a zero-pixel shift and completely suppress possible stray light with a special design so that the background of the fluorescence image appears completely dark. A selection of common fluorescence filters for materials analysis follows below. Additional fluorescence filters can be found on: Filter system A Filter system A Filter system D Filter system E Filter system I Filter system N Filter system N Filter system B/G/R Filter system CFP Filter system YFP

27 Transmitted-light illumination The mount for the transmitted-light illumination arm is a fixed component of the Leica DMI 3000 M/DMI 5000 M basic stand. Pressing the IL/TL key switches from incident-light to transmitted-light illumination in less than 1 second. The transmitted-light arm as well as the condenser and filter are ordered separately. The transmitted-light illumination unit essentially consists of an illumination and condenser carrier. Its excellent light utilization is ensured by replaceable Leica lamp housings and optimized aspherical collectors. Optimal and homogenous illuminated areas are a requirement for all transmitted-light contrasting methods, such as bright field, dark field, phase or interference contrast (ICT). The transmitted-light illumination for the DMI 3000 M/DMI 5000 M consists of the following components: Manual, coded transmitted-light illuminator arm (incl. light filter mount) Transmitted-light lamp housing Manual condenser S70/0.30 or Motorized condenser S28/0.55 Manual transmitted light axis (for DMI 3000 M) (Fig. 61 p. 27) for 100 W lamphouses with integrated tilting mechanism with CQC "Condenser Quick-Changer" for all manual condensers Manual field diaphragm Manual shutter Manual transmitted light filter magazine Lamp housing mount for interchangeable lamp housings 100W With integrated duct for the Lamp house cable Fig. 60: Lamp mount Fig. 61: Transmitted-light arm Manual transmitted light axis with integrated 30W lamp house (for DMI 3000 M) (Fig. 62 p. 27) with integrated tilting mechanism with CQC "Condenser Quick-Changer" for all manual condensers Manual shutter Manual transmitted light filter magazine Fig. 62: Transmitted light arm 30 W 27

28 Fig. 63: Stand with transmitted-light illumination arm Fig. 64: Transmitted-light filter Manual, coded transmitted-light illumination arm (for DMI 5000 M) (Fig. 61 p. 27) Compact transmitted-light illumination arm with integrated tilting mechanism with integrated manual field diaphragm with integrated manual filter magazine for 2 replaceable filter positions one position factory-equipped with a shutter with condenser quick-change with automatic condenser identification with standard lamp housing changer for all Leica lamp housings with integrated duct for lamp housing cable Light filter dia. 40 mm, unframed for transmitted-light illumination arm 2 transmitted-light filters can be swung in on the manual transmitted-light illumination arms. A broad selection of different filters is available to optimize illumination for observation and documentation. DLF, daylight filter (blue, conversion filter e.g. for daylight film and/or visual observation) ALF, artificial light filter (red, correction filter for artificial light film) Panchromatic green filter Neutral filter N 16 (6,3%) Neutral filter N 4 (25%) Neutral filter N 2 (50%) VG 9, narrow band filter

29 Condensers and accessories The Leica DMI 5000 M offers a selection of different condensers. All condensers feature a 7-position condenser disk to accommodate IC prisms or light rings. They can be individually equipped and are easy to use. All condensers can be used for bright field, dark field, phase, polarization and interference contrast and feature an aperture diaphragm a mount for interchangeable condenser heads a mount for a polarizer a filter mount with a diameter of 32 mm The S70/0.30 condenser features a free working distance of 70 mm and a numerical aperture of 0.30 and is particularly suitable for high-volume specimens at magnifications of up to 40x. An auxiliary lens is swung in for magnifications of 1.25x to 5x to provide homogeneous illumination all the way to the edge of the entire 25 mm field of vision. The S28/0.55 condenser features a free working distance of 28 mm and a numerical aperture of 0.55 and offers the best resolution and magnifications of up to 100x. Specimens with a height of up to 28 mm can be examined in transmitted light. In addition to the standard contrast methods, this condenser is also suitable for dark field contrast at objective apertures of up to Fig. 65: S70 condenser Fig. 66: S20 condenser S70 condensers Manual Coded manual condenser including head S70/ Light ring set for phase contrast S70/ Manual polarizer holder including polarizer on 32/43 mm adapter for Wollaston prisms Wollaston prisms for DIC see page 23 S28 condensers Manual with fixed condenser head Coaded manual condenser baser with fixed mount for all condenser heads, S1 S Condenser head S28/ Light ring set for PH and DF contrast S23 S Motorized polarizer holder including polarizer Wollaston prisms for DIC see page 23 Fig. 67: S1 condenser Fig. 68: S28 condenser head Focusing telescope (Fig. 69 p. 29) A focusing telescope is required when adjusting phase contrast (ICT) or differential interference contrast in order to view the rear focal plane of the objective. Focusing telescope dia. 30 mm Fig. 69: Focusing telescope 29

30 Lamp mount for lamp housings as well as fiber optic or laser couplings Depending upon the respective application, it is possible to adapt different lamp housing mounts for up to 3 ports at the rear side of the stand. The following distinctions apply: Fig. 70: Motprized mirror housing (open) Lamp mount for one lamp housing, or a fiber optic or laser coupling Ergonomically angled 90 to the right for optimal adjustment of lamp housings Lamp mount for one lamp housing, or a fiber optic or laser coupling Under 0 for straight-through coupling Manual mirror housing to accommodate two light sources, or fiber optic or laser couplings. Ergonomically angled 90 to the right for optimal adjustment of lamp housings and a straight position Fig. 71: Booster lens Manual mirror housing to accommodate two light sources, or fiber optic or laser couplings. Ergonomically angled 90 to the left for optimal adjustment of regular lamp housings and a straight position Motorized mirror housing (for DMI 5000 M) (Fig. 70 p. 30) to accommodate 3 light sources, or fiber optic or laser couplings. Ergonomically angled 90 to the left and right for optimal adjustment of all Leica lamp housings and a straight position Fig. 72: Reflector cube for lamp adjustment Booster lens (for DMI 5000 M) (Fig. 71 p. 30) Depending on the application, a 180 turn of the switchable auxiliary lens (booster) provides either maximum fluorescence intensity (factor: 1.4x in 75% center of field) or optimal homogeneity and brightness distribution (factor: 0.7x for the entire field). Suitable for insertion into the stand on the right or left, depending on order Reflector cube for lamp adjustment (Fig. 72 p. 30)

31 Light sources, lamp housing, supply units The light sources are housed in lamp mounts, which are housed in lamp housings. The lamp housings are fastened to the stand or transmitted-light illumination arm using a flange ring. This allows optimum heat decoupling and comfortable handling. The following equipment can be used: For incident light and transmitted light: Standard lamp housing Lamp housing 107/2 (single-lens) (Fig. 73 p. 31) Plastic lamp housing with lamp access from above. With fixed, pre-centered lamp mount with 0.70 m power cable including 1x halogen bulb 12 V 100 W, single-lens aspherical, permanently set collector, heat-absorbing filter, microprism grid disk with middle diffuser for enlargement of the lamp filament and optimization of the illuminated area, without reflector, without replacement lamp Fig. 73: Lamp housing 107/2 (single-lens) Lamp housing 107/2 (single-lens) (as in Fig. 73 p. 31) as above, but with 2.5 m power cable Lamp housing 107, left-hand operation (double-lens) (Fig. 74 p. 31) Plastic lamp housing with lamp access from above. With centerable lamp mount with 0.70 m power cable including 1x halogen bulb 12 V 100 W, double-lens aspherical, focusable collector, heat-absorbing filter, microprism with middle diffuser for enlargement of the lamp filament and optimization of the illuminated area, without reflector, without replacement lamp Fig. 74: Lamp housing 107 (double-lens) Lamp housing 107, left-hand operation (double-lens) (as in Fig. 74 p. 31) as above, but with 2.5 m power cable Lamp housing 106 (double-lens) (Fig. 75 p. 31) Metal lamp housing with side lamp access. With centerable lamp mount with 0.70 m power cable including 1x halogen bulb 12 V 100 W, double-lens aspherical, focusable collector, heat-absorbing filter, microprism with middle diffuser for enlargement of the lamp filament and optimization of the illuminated area, without reflector, without replacement lamp Lamp housing 106 (double-lens) (as in Fig. 75 p. 31) as above, but with 2.5 m power cable Fig. 75: Lamp housing 106 (double-lens) 31

32 Lamp housing 106z, left-hand operation 12 V 100 W (4-lens) (Fig. 76 p. 32) Metal lamp housing with side lamp access. With centerable lamp mount with 0.70 m power cable including 1x halogen bulb 12 V 100 W, four-lens, achromatic, focusable collector, centerable reflector for doubling of the lamp filament and optimization of the illuminated area, with heat-absorbing filter, without auxiliary lamp Fig. 76: Lamp housing 106z 12 V 100 W (4 lens) Lamp housing 106z 12 V 100 W (4 lens) (as in Fig. 76 p. 32) As , but right-hand operation For fluorescence Lamp housing 106z, left-hand operation Hg 100 W (6-lens) Metal lamp housing with side lamp access. Left-hand operation for DMI 5000 M. With centerable lamp mount for Hg 100 W lamp with 1.5 m power cable. 6-lens, achromatic 1-inch collector, UV-optimized transmission > 50% at 340 nm, centerable reflector for doubling the focal point and optimization of the illuminated area, with heatabsorbing filter, without burner Fig. 77: Halogen bulb 12 V, 100 W for lamp housing 106z Lamp housing 106z Hg 100 W (6-lens) As , but right-hand operation Lamp housing 106z Xe 75 W (6-lens) Metal lamp housing with side lamp access. With centerable lamp mount for Xe 75 W lamp with 1.5 m power cable. 6-lens, achromatic 1-inch collector, UV-optimized transmission > 50% at 340 nm, centerable reflector for doubling the focal point and optimization of the illuminated area, with heatabsorbing filter, face protection, protective gloves, without burner Fig. 78: Lamp mount for Hg 50 W Fig. 79: Lamp mount for Hg 100 W Fiber-optics coupling A fiber-optics coupling can be connected between lamp mount and lamp housing. This "cold" light source prevents the stand from heating up, which makes it particularly useful for long-term experiments. Fiber-optics coupling complete with 1 m fiber Fiber-optics coupling complete with 2 m fiber Lamps and burners Halogen bulb 12 V 100 W (Fig. 77 p. 32) High-pressure mercury burner Hg 100 W (Fig. 79 p. 32) High-pressure mercury burner Hg 100 W/2 (longer life) Lamp HXP R120/45C-Vis for Leica EL High-pressure xenon burner Xe 75 W

33 Spacer for filter D = 50 mm (Fig. 80 S. 33) 2 filter positions at LH 106/107, 4 filter positions with LH 106 z. Adapter between LH Additionally, for Xe 75 W lamp: Gray filter 0.2% D = 50 mm, 0.2% transparency, in holder Diffusion filter N D = 50 mm, embossed, in holder Additional filters D = 50 mm in holder: DLF, daylight filter ALF, artificial light filter Panchromatic green filter VG 9, green filter Neutral gray filter N20 (5%) Heat-absorbing filter Fig. 80: Filter and spacer for filter d = 50 mm Supply units For power supply voltages other than V, the following is required: Pre-transformer V With power supply cord, primary V, secondary V Fig. 81: Polarizer protective filter Supply unit Hg 100 W (Fig. 82 S. 33) With power supply cord, automatic switching to power supply voltage 90 V 250 V 50/60 Hz with operating hours display Supply unit Xe 75 W With power supply cord, automatic switching to power supply voltage 90 V 250 V 50/60 Hz with operating hours display Leica EL6000 (Fig. 83 S. 33) External light source Liquid light guide, 2 m inch fiber optics adapter for lampmounts inch fiber optics adapter direct at the DMI Cable for shutter control EL6000 I 2 C Fig. 82: Supply unit Hg 100 W Abb. 83: Leica EL

34 TV adapter Fig. 84: TV adapter You can adapt analog and digital cameras to all tubes with documentation output. The C-B and F-mount adapters are aligned to the dimensions of the holder thread. The various fixed and variable magnification factors allow adjustment of the rendering of the microscopic image on the camera chip. In order to display the largest possible portion of the field of view on the monitor, the magnification factor of the adapter must fit the chip size of the camera. If the magnification is too low, there will be a lack of uniformity to the illuminated area (shading) and/or vignetting. Fig. 85: TV adapter Diagonal measurements of images taken (in mm) at 1 inch- 2/3 inch- 1/2 inch- 1/3 inch- Order No. camera camera camera camera Without variable magnification, only for single chip cameras: C-mount adapter 1x HC C-mount adapter 0.7x HC C-mount adapter 0.55x HC 14, C-mount adapter 0.35x HC With variable magnification (vario TV adapter) for 1 3 chip cameras: C-mount, x HC 19* B-mount (ENG), x HC (1/2 inch) Without variable magnification level, only for 1-3 chip cameras: C-mount adapter 1x B-mount adapter 1x B-mount adapter 1.25x F-mount adapter 1x F-mount adapter 1.25x required for each: TV optics 0.5x HC * available beginning with vario factor 0.42x! Fig. 86: C-mount 0.63x HC Fig. 87: C-mount 0.5x HC 34

35 Objectives, objective turret, optics Objectives The optics are the heart of every microscope and are decisive for the quality of the image information. Leica has set standards in this respect with the introduction of HC optics. Eyepieces, tube lenses and objectives have been carefully harmonized with one another. Based on the Leica principle of infinity distance correction of optics, the microscope objectives are infinity corrected for tube lens systems with 200 mm reference focal lengths. The calibration length is 45 mm for bright field. The objectives are divided into 4 correction classes: Achromatic objectives: HI PLAN Field of view performance up to FOV 20 Planachromatic objectives: N PLAN Field of view performance up to FOV 22 Semi-apochromatic objectives: HC/X PL FLUOTAR Field of view performance up to FOV 25 Apochromatic objectives HC/X PL APO Field of view performance up to FOV 25 When selecting the objectives, consider the intended use with regard to specimen covering, etc. For applications in the industrial field, this cover glass correction is "0". For more detailed explanations, please refer to Fig. 88: Objective turret Fig. 89: Adapter for RMS-threaded objectives Objective turret In principle, all infinity corrected high-performance Leica objectives with an M32 or M25 thread may be used. Even older objectives can be adapted for further use. Adapter rings for objectives with RMS or M25 thread are also available. Objectives of earlier lines with RMS threads cannot be adapted unconditionally, however, as problems with parfocality and field flattening may arise. Adapter M32/M Adapter M32/RMS Numerous application objectives with long working distances (L objectives) are available specifically for inverted microscopy. The following table lists all objectives of the Leica program. The focusing of the specimen is carried out via the objective turret that can be fitted with up to 5 objectives (M32). The reliable, stable and precise focusing is not affected by the stage and its specimens or accessories. 35

36 Accessories Immersion oil, 10 ml Free of natural fluorescence as per ISO 8036/1, refraction index n e 23 = ± 0.005, dispersion v e 23 = 44 ± Fig. 90: Screw cap Immersion oil, 20 ml as per ISO 8036/1, refraction index n e 23 = ± 0.005, dispersion v e 23 = 44 ± Immersion oil, 250 ml as per ISO 8036/1, refraction index n e 23 = ± 0.005, dispersion v e 23 = 44 ± Stage micrometer Transmitted light 2 mm = 200 parts Stage micrometer Incident light 1 mm = 100 parts Focusing telescope dia mm Halogen lamp 12 V 100 W Fig. 91: DIC cover Hg 50 W burner Hg 100 W/2 burner Xe high-pressure burner Screw cap for unused objective mounts (Fig. 90 p. 36) Component of stand Cover for unused objective DIC-disk opening (Fig. 91 p. 36) Component of stand Fig. 92: Analyzer opening cover Fig. 93: Camera port cover Dust and light excluder for analyzer opening (Fig. 92 p. 36) Component of stand Dust and light excluder for camera port opening (Fig. 93 p. 36) Component of stand Ergonomic height compensation plate To increase the viewing height by 20 mm, or to increase the lateral camera ports for oversized cameras, a height compensation plate was developed. Height compensation plate: 12 mm Height compensation plate: 25 mm

37 Anti-vibration Anti-vibration table Anti-vibration table for DMI series with massive marble insert. The insert comes with a hole to use the Leica DMI stands with bottom-port Vibration Isolation Platform (Fig. 94 p. 37) VIP Series 3000 from Vistek TM for DMI stands to isolate the microscope from external vibrations. The platform is designed for routine tabletop use Digital image documentation Leica digital camera system DC Monochrome and color digital cameras for all requirements between highest resolution (12 megapixels) and quick live image (25 frames per second) (see special brochures) Fig. 94: Vibration Isolation Platform Software The LAS (Leica Application Suite) is the PC interface for the configuration, operation, evaluation and storage of microscope data and images. LAS, Basis Package Weitere Module: LAS Steel Expert Auto - for fully automatic steel inclusion rating LAS Steel Expert Manual - manual steel inclusion rating LAS Reticule Leica Materials Workstation Environment - basic module of Materials Workstation Leica QHardness interactive Leica QParticles - for particle analysis Leica Metallurgy Suite Includes the following 5 modules :Part numbers indicated for these modules refer to the individual module Leica QGrain - grain size analysis Leica Graphite - for the determination of nodular graphite Leica Phase Percent - measuring of phase percent Leica Coating Thickness - determination of coating thicknesses Leica Depth - for the determination of decarburisation Fig. 95: Leica DFC Camera 37