Turner Spectrophotometer
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1 BARNSTEAD THERMOLYNE CORPORATION Turner Spectrophotometer SP-870 OPERATION MANUAL LT1102X6 Model # SM SM Voltage 115V 230V with European cord set 6/97 1
2 2
3 Table of Contents Introduction... 4 Safety Information... 4 General Usage... 4 Theory... 5 Working Principle... 7 Specifications... 8 Instrument Description... 9 Front Panel Description... 9 Keyboard Rear Panel Description Environmental Conditions Unpacking Standard Equipment General Cleaning Operation Pre-operation Check Procedures Startup Concentration Mode Factor Mode Memory Setup Auxiliary Function for Memory Setup Selection of Memory Mode Printout of Memory Users Functions Stand-by mode I.D. Number Setting Mode Wavelength Check Mode Maintenance Replacing the Lamp Realigning the Lamp RS-232 and Printer External Output Setup External Output Message Code Interpretation Warranty
4 Introduction Congratulations on your purchase of a Turner Spectrophotometer. Warning Warnings alert you to a possibility of personal injury. Caution Cautions alert you to a possibility of damage to the equipment. Note Notes alert you to pertinent facts and conditions. Introduction SP-870 is a digital microprocessor-controlled visible spectrophotometer providing photometric read-out of Transmittance, Absorbance, Concentration and converted Factor with the following unique features. - automatic filter selection - auto zero - internal wavelength calibration - error messages - RS-232C/printer interfaces - 20 x 2 LCD display - (30) user-programmed text modes The unit is designed to do a number of jobs within your laboratory. Please read the instructions carefully to ensure that you receive maximum benefit from it. Also, be sure to fill out and return the enclosed warranty registration card. We would like to receive the information requested, and it will help us assure you of proper warranty coverage. Safety Information Your Turner Spectrophotometer has been designed with function, reliability, and safety in mind. It is the user's responsibility to install it in conformance with local electrical codes. For safe operation, please pay attention to the alert boxes throughout the manual. 4 General usage Do not use this product for anything other than its intended usage.
5 INTRODUCTION Theory A spectrophotometer is primarily used to identify unknown substances and determine their concentrations. The following principles outline how this is accomplished. Matter absorbs energy when it interacts with an energy source such as light. Due to its distinctive atomic structure, each substance only absorbs energy between certain levels. Since energy is inversely proportional to wavelength, E=hc/ λ (where h is Plank s constant, c is the speed of light, and λ is the wavelength of light), every substance has a characteristic absorption spectrum. For example, hydrogen absorbs light at the following wavelengths in the visible region: 410.7nm, 433.8nm, 485.7nm and 657.9nm. We can verify the existence of hydrogen in an unknown sample by comparing the wavelengths actually absorbed by the unknown sample to hydrogen s known absorption spectrum. Fig. 1 Therefore, a device, namely your spectrophotometer, is required to measure absorption spectrums in order to identify an unknown substance. A spectrophotometer measures the amount of light absorbed at distinct wavelengths of incident light. As a result, a graph of the amount of absorbed light at various wavelengths can be plotted (absorbance vs. wavelength, as shown in Fig. 1.) This is known as an absorption spectrum. Such a graph can be used to identify the presence of a particular substance. The concentration of an unknown sample can also be determined. When a light beam is incident to a sample, part is absorbed and part is 5
6 INTRODUCTION transmitted. The transmittance (T) is defined as the ratio of the transmitted intensity of the light beam (I t ) to the initial intensity of the light beam (I o ), or T= I t / I o. The absorbance (A) is defined as A=log (1/T). It is found that absorbance is directly proportional to concentration: A= ε b c where A is the absorbance, c is the concentration (mol/l), b is the pathlength of the sample (cm), and ε is a constant called the molar absorptivity (L/mol-cm). This linear relationship between absorbance and concentration is called Beer s Law. Fig. 2 Standard Curve (Beer s Law) Fig. 2 Standard Curve (Beer s Law) The concentration of an unknown sample can be determined by using Beer s Law and preparing a standard curve. A standard curve is prepared by measuring the absorbance of a series of known standards at different concentrations. The absorbance vs. concentration is then plotted for each standard (represented by x in Fig. 2). A linear relationship between the absorbance and concentration should then be observed (straight line in Fig. 2) and a linear standard curve is thus prepared. It is then possible to determine the concentration of an unknown sample by measuring its absorbance and comparing it to the standard curve. The concentration of the unknown can then be determined as shown by the dotted line in Fig. 2. Therefore, if we have a standard sample with a known absorbance and concentration, it is easy to determine an unknown concentration of the same substance by measuring its absorbance and applying Beer s Law. 6
7 INTRODUCTION Light Source Mono chomator Sample Compartment Detector Display Working Principle A spectrophotometer basically consists of five parts: a light source to supply electromagnetic energy, a monochromator to isolate the wavelength of interest and a stray light filter to eliminate the unwanted second order radiation, a sample compartment to accommodate the sample solution, a detector to receive the transmitted light and convert it to electrical signal, and either a digital or analog display to indicate absorbance and transmittance. The block diagram to the left illustrates the relationship among these parts. In your spectrophotometer, a light beam from the lamp is focused on the entrance slit of the monochromator and passes into the monochromator, where the collimating mirror directs the beam to the grating. The grating disperses the light beam to produce the spectrum, a portion of which is focused onto the exit slit of the monochromator by the collimating mirror. From here the beam is passed through a stray light filter which helps eliminate unwanted second order radiation. The beam then enters the sample compartment where it is passed through the sample. The sample may absorb a portion of the light beam. Upon leaving the sample compartment, the beam then falls on the silicon diode detector. The intensity of the light beam causes the detector to produce an electrical signal. This electrical signal is then converted to transmittance or absorbance and displayed. 7
8 INTRODUCTION Specifications Wavelength Range nm Bandwidth Pass 5nm (nominal) Wavelength Accuracy +/- 2nm Wavelength Repeatability +/- 0.5nm Wavelength Setting Automatic Zero & Blank Setting Automatic Filter Selection Automatic Display 20 x 2 LCD Modes Transmittance 0~100%T Absorbance 0.000~2.000A Concentration 0~19,999 Factor 1~19,999 Photometric Accuracy 1% at 1A Photometric Linearity 1% Stability Signal Processing Stray Light Cuvettes Drifting 0.003A/hr after half hour warmup at 0A Digital, Microprocessor 0.1%T typical at 340nm, <0.5%T nm A. Round cuvettes 10-13mm in diameter; B. Square cuvettes 12.5 x 12.5 x 45mm, 1 cm path length (optional-34f01-04); C. Vacuum empty cuvette (optional- AYX9) Power 115/230V~. 50/ 60Hz (switchable) Analog Output 0.00~1.00V Interface RS-232C, Printer Dimension 400mm(W) x 300mm(D) x 145mm(H) Weight 9.0kgs 8
9 INTRODUCTION Instrument Description Front Panel Description 1. DISPLAY panel - Displays wavelength, %T, ABS, CONC, or FACTOR value. 2. Power indicator - Indicates power ON/OFF and STANDBY mode. the indicator lights in green when the power is on for operation and will turn red when the instrument in under STANDBY status. 3. SAMPLE Compartment - For round cuvettes or square cuvettes. 4. Keyboard - For data entry. See Keyboard information, next page, for details. 9
10 INTRODUCTION Keyboard 1. FUNC A. F0: Standby Mode B. F1: ID number setting C. F2: Wavelength Check %T/OA - Sets the instruments to 100% Transmittance or Zero Absorbance. 3. PRINT - Directs the output to RS-232C and Printer interface. (*See dip switch setting on the rear panel in the enclosed appendix.) 4. λ nm - Sets desired wavelength. 5. A - Selects Absorbance mode. 6. T - Selects Transmittance mode. 7. C - Selects Concentration mode. 8. F - Selects Factor mode. 9. SHIFT + Any Arrow Key - Searches memory mode in memory setting or reading. 10. SHIFT + C - Enters memory reading. 11. SHIFT+ F - Enters memory setting. 12. ESC - Clear input or quit operation mode Numerical value Decimal point 15. ENTER - Enters the keyed in numerical values into the system. 10
11 INTRODUCTION Rear Panel Description 1. RS 232-C 2 Printer 3. Analog Output 4. Fan 5. Power Switch 6. Fuse 7. Power Socket V/230V Voltage Switch Environmental Conditions Operating: 17 C - 27 C; 20% - 80% relative humidity, non-condensing. Installation Category II (over-voltage) in accordance with IEC 664. Pollution Degree 2 in accordance with IEC 664. Altitude limit: 2,000 meters. Storage: -25 C - 65 C; 10% - 85% relative humidity. 11
12 INTRODUCTION Unpacking The package contains the items stated below. Unpack the carton and inspect carefully. If any part is damaged or missing, contact your dealer immediately. Save the packing materials in case the unit may need to be repacked or returned for service. Standard Equipment QTY DESCRIPTION PART # 1 SP870 Spectrophotometer 115V SM V SM Power cord 115V CRX72 230V CRX70 6 Round borosilicate cuvettes 34F Fuse 115V/1.0 Amp Fuse 230/0.5 Amp FZX35 1 Operator s manual LT1102X6 General cleaning instructions Wipe exterior surfaces with a dampened cloth containing a mild soap solution. 12
13 Operation Pre-operation check procedure Your spectrophotometer has been fully checked and aligned before shipping. However, a short pre-check should be performed before the first use of this instrument. 1. Carefully unpack your spectrophotometer and check every item against the Standard Equipment list. 2. Place the spectrophotometer on a solid and level surface in a dry, clean environment. 3. Confirm that the correct line voltage is set and correct fuse is installed. 1.0A for 115V, or 0.5A for 230V. 4. Plug the AC power cord into the rear of the unit and plug into a grounded wall socket. 5. Turn the power ON by switching the rocker switch at the rear of the spectrophotometer. 6. Allow the spectrophotometer to warm up for at least 20 minutes to stabilize the electronic components. 7. Set the wavelength dial to 340nm. Press the 100% T/0A button. The display should show while in the TRANS (transmittance) mode and for the ABS (absorbance) mode. Indicator lights below the main display indicate whether the unit is in the transmittance or in the absorbance mode. To change the mode setting, press the T button for transmittance or the A button for absorbance on the front panel of the unit. 8. If the LED display shows Err3 or Err4, the lamp alignment may have shifted during shipment. Refer to the section entitled Realigning the 13
14 OPERATION Lamp for more information. Startup 1. Turn the power on. A complete initialization calibration of the instrument for electronic checks and Zero Order alignment will be automatically performed. This process will take approximately 2 minutes. The display will run through: MODEL 870 VERSION: 1.6 AB MODEL 870 SYSTEM TEST PASS 0 MODEL 870 ** CALIBRATION** 2. After the calibration is done the system will directly enter the first memory mode which is pre-set at 440nm and F1. The display should show: 440 T WAITING BLANK The instrument is now ready for use. Refer to details in related sections. 14
15 OPERATION Operation procedures Turn the power on, and allow the instrument to warm up for at least 20 minutes. After an internal self-test for initializaiton calibration of the instrument is performed, the system will proceed to first memory mode automatically. The first memory mode is pre-set for wavelength at 440 nm and for Factor at 1 when the instrument is delivered. The display should show: 440 T WAITING BLANK Note It is suggested to run the Wavelength Check Mode to ensure the nm position after a large number of samples have been run or after stand-by mode. Percent Transmittance and Absorbance mode 1. Fill a cuvette with the solvent that will be used to zero the spectrophotometer. This is the blank (or background) solvent that your sample is dissolved in. 2. Place the cuvette in the cuvette holder in the sample compartment. Close the sample compartment lid. 3. Press (λ nm) button, enter the numerical value ( ) of the wavelength and press the ENTER button. If a mistake is made in the key-in process, press the ESC button to clear the display for a new entry. 4. Select Transmittance or Absorbance mode by pressing the (T) or (A) button. 15
16 OPERATION 5. Zero the instrument using the blank solution by pressing the (100%T/OA) button. The display should show: XXX TCALB or XXX ACALB then should read XXX 100.0T or XXX 0.000A (Transmittance mode) (Absorbance mode) 6. Carefully open the sample compartment and remove the cuvette. 7. Place the cuvette with solution to be measured in the sample compartment and close the sample compartment door. The cuvette should be oriented in the same position as the cuvette used to zero the instrument with the blank solution. The display will show the Transmittance or Absorbance immediately. Record the value and remove the cuvette. 8. Repeat step 7 for further samples to be tested at the same wavelength. For a different wavelength, steps 1 through 7 should be repeated. 9. After the test is complete, turn off the power; or, set the instrument at Standby mode if further test will be performed soon. (Refer to Standby mode section.) 16
17 OPERATION Concentration Mode 1. Fill a cuvette with the solvent that will be used to zero the spectrophotometer. This is the blank (or background) solvent that your sample is dissolved in. 2. Place the cuvette in the cuvette holder in the sample compartment. Close the sample compartment lid. 3. Press (λ nm) button, enter the numerical value ( ) of the wavelength and press the ENTER button. If a mistake is made in the key-in process, press the ESC button to clear the display for a new entry. 4. Select Transmittance or Absorbance mode by pressing the (T) or (A) button. 5. Zero the instrument using the blank solution by pressing the (100%T/OA) button. The display should show: XXX TCALB or XXX ACALB then should read XXX 100.0T or XXX 0.000A (Transmittance mode) (Absorbance mode) 6. Carefully open the sample compartment and remove the cuvette. 7. Fill a cuvette with a Standard solution of known concentration and place the cuvette in the sample compartment. 17
18 OPERATION 8. Select Concentration mode by pressing (C) button and key in the concentration of the Standard solution. Press the ENTER button. The display should show: XXX OC XXX XXXXXC 9. Place the cuvette with unknown solution to be measured in sample compartment and close it. The display will show the Concentration of the solution immediately. Record the value and remove the cuvette. 10. Repeat set 9 for further samples to be tested at the same wavelength. For a different wave length, steps 1 through 9 should be repeated. Note 11.After the test is completed, turn off the power; or, set the instrument to STANDBY mode if further tests will be performed soon. (Refer to Standby mode section.) 1. If a mistake is made in the keyin, press ESC button to clear for new entry. 2. The Standard value range is If so desired, press (F) button and record the calculated FACTOR value in memory to enable measurement with same reagent to be made at later time without restandardization. The calculated FACTOR value range should be at Factor mode 1. Fill a cuvette with the solvent that will be used to zero the spectrophotometer. This is the blank (or background) solvent that your sample is dissolved in. 2. Place the cuvette in the cuvette holder in the sample compartment. Close the sample compartment lid. 3. Press (λ nm) button, enter the numerical value ( ) of the wavelength and press the ENTER button. If a mistake is made in the keyin process, press the ESC button to clear the display for a new entry. 18
19 OPERATION 4. Select Transmittance or Absorbance mode by pressing the (T) or (A) button. 5. Zero the instrument using the blank solution by pressing the (100%T/OA) button. The display should show: XXX TCALB or XXX ACALB then should read XXX 100.0T or XXX 0.000A (Transmittance mode) (Absorbance mode) 6. Carefully open the sample compartment and remove the cuvette. 7. Place the cuvette with solution to be measured in sample compartment and close the sample compartment lid. 8. Select Factor mode by Pressing (F). The display should show: XXX 1 FACTOR: 9. Key in factor value ( ) and press the ENTER button. (Refer to Memory Reading.) 10.System will automatically shift to Concentration mode, and the display will show the Concentration of the Solution. This calculated concentration is a result of the measured Absorbance multiplied by the entered (or pre-stored) factor value. Record the 19
20 OPERATION value and remove the cuvette. The multiplied Concentration value range should be If this is exceeded, re-define the factor value. 11. Repeat steps 7 & 10 for further samples to be tested at the same wavelength and factor. For a different wavelength or factor, steps 1 through 10 should be repeated. 12. After the test is complete, turn off the power; or, set the instrument at Standby mode if further testing will be performed soon. (Refer to Standby mode section.) Note To exit Memory Setup mode press ESC. Memory Setup SP-870 provides thirty (30) user-programmable memory test modes. To enter MEMORY SETUP, press the SHIFT and F buttons simultaneously. The display should show: >01 440NM F XXXXXX 02 NONE Each memory comprises four items: 1. > sign that indicates current memory mode 2. memory mode number (01-30) 3. wavelength 4. factor value Memories normally are undefined when the instrument is delivered. An undefined memory is followed with None. 1. Press the ENTER button to set up the current memory mode that the > sign indicates Key in the desired data respectively for wavelength and factor value, each key-in should be followed by pressing the ENTER button.
21 OPERATION >2 NONE WAVELENGTH : XXX e.g. WAVELENGTH = 405nm Note 1. Re-setup on the defined memory mode can be done in the same way as setup, but previous data will then be replaced with the new input. >02 405NM FACTOR : XXXXXX >02 405NM F NONE e.g. FACTOR = Setup of undefined memory mode should be done in serial order (i.e. 1,2,3,4,5,...). Skip over or random setup will not be accepted by the system. Auxiliary function for memory setup When in memory setup mode, press FUNC button to use this function. The display will respond as below: <XX> 1. END 2. COPY 3. DELETE 4. INSERT <xx> Current memory mode number 1. End: Press (1) button to proceed to the first undefined memory mode 2. Copy: Press (2) button to copy the system-installed wavelength (440 nm) and factor (1) to current memory mode. 3. Delete: Press (3) button to delete the current memory mode 21
22 OPERATION 4. Insert: Press (4) button to insert a blank (undefined) mode on to the current memory mode Selection of memory mode To select or search the desired mode in memory setup or reading mode, the options are as follows: 1. Press SHIFT + buttons to proceed to last mode 2. Press SHIFT + buttons to proceed to next mode 3. Press SHIFT + buttons to proceed to last page 4. Press SHIFT + buttons to proceed to next page 5. Key in the memory mode number (e.g. 5), and press the ENTER button. The desired memory mode will be available on display. For example: >01 XXX NM F XXXXXX 02 XXX NM F XXXXXX Key in (5) >01 XXX NM F XXXXXX READ MEMORY : 5_ Press ENTER >05 XXX NM F XXXXXX 06 XXX NM F XXXXXX 22
23 OPERATION Printout of memory To obtain a hard copy of the memories, proceed as follows. Be sure the printer interface is set to ON. For printer setup see External Output Setup. 1.When in memory setup mode, press the PRINT button and the display should show: 1. MEMORY PRINTOUT 2. INTERFACE SETUP 2. Press (1) to print out the memories. The display should show as below when the printout is in process. 1. MEMORY PRINTOUT BUSY After the printout is done the system will return to memory setup mode. User s Function SP-870 provides three (3) user s function modes, which are: Mode - 0: Stand by Mode - 1: I.D. number setting Mode - 2: Check Wavelength Stand-by mode This mode is used to turn off the light source, and still keep the electronic parts ON status when the operation is supposed to be temporarily discontinued. 23
24 OPERATION Note After entering the User s Function mode by pressing FUNC button, the display should respond with: a: User s function 2 (Wavelength Check) before blanking is done; or, b: User s function 1 (I.D. number setting) after blanking is done. 1. When in test mode, press FUNC button. The display should show: USER S FUNCTION: 2 CHECK WAVELENGTH OR USER S FUNCTION: 1 I.D. NUMBER SETTING 2. Press (0) and ENTER buttons, and the display should show: USER S FUNCTION: 0 STANDBY MODE Note Allow the instrument to warm up for at least 20 minutes to stabilize the light source before use. STANDBY MODE ANY KEY TO QUIT Under stand-by mode, the power indicator will turn RED. 3. To quit stand-by mode, press any button. The system will return to test mode. I.D. Number Setting Mode This mode is used to assign an I.D. number on the printout of test results. This mode can only be entered after blanking is done. 1. After blanking is done, press FUNC button and the display should show: USER S FUNCTION: 1 I.D. NUMBER SETTING 24
25 OPERATION 2. Press the ENTER button and the display should show: I.D. NUMBER SETTING NOW - 01 NEW = The I.D. number is pre-set to start from 01; however, user-defined starting number (00-99) is acceptable. Re-assignment of I.D. number during test can also be done. When this mode is selected, printout setup for I.D. number will be automatically set to YES. (See external output setup.) 3. Key in the desired starting I.D. number (if it is not 01), and press ENTER button to continue the test. Wavelength check mode This mode is used to check the wavelength at 000.0nm. 1. When in text mode, press FUNC button, and the display should show: USER S FUNCTION: 2 CHECK WAVELENGTH or USER S FUNCTION: 1 I.D. NUMBER SETTING 25
26 OPERATION 2. Press ENTER button if the display is on User s function 2, or press (2) and ENTER buttons if the display is on User s function: 1. The display should become: WAVELENGTH CHECK NM CHECKING 3. The checking at nm is in process. 4. If the wavelength is checked OK, the display should show. WAVELENGTH CHECK CHECK PASS Then, press ESC button to quit, and the system will return to test mode. 5. If the wavelength is checked not in specification, the display should show: WAVELENGTH CHECK CHECK ERROR 6. Press SHIFT and ESC buttons simultaneously to re-align the wavelength at nm. The display should show as below when the realignment is in process. Then, the system will return to test mode. SP-870 ** CALIBRATION** 26
27 Maintenance Caution Unplug your spectrophotometer before replacing the lamp. Replacing the Lamp 1. Open the door on the right hand side of the spectrophotometer. Push and twist counterclockwise to remove the lamp from the holder. 2. Insert the new lamp into the socket and carefully rotate clockwise until it locks into position. 3. Carefully wipe fingerprints off the lamp with a small tissue. 4. Proceed to Realigning the Lamp. Caution The lamp is hot when operating. Wait a few minutes before touching the lamp. Realigning the Lamp It is necessary to realign the light bulb after each transportation or replacement. 1. Turn on the power and the display should show: MODEL -870 SYSTEM TEST PASS 0 then press FUNC button and the display should show: ADJUST LAMP LOCATION ** CALIBRATION** 2. Wait approximately one minute and the display should show: ADJUST LAMP LOCATION 26 ADC VALUE: XXXX_ 27
28 MAINTENANCE 3. Open the door on the right hand side and move the lamp holder forward or backward until the highest value is obtained. The value range should be Press ENTER button to recheck. If the value is out of range, repeat step Press ESC button to leave the function and enter system calibration; the display should show: MODEL -870 VERSION 1.6 AB The instrument will then continue through the initialization calibration routine. 28
29 RS-232 and Printer External output setup SP-870 provides built-in analog output, RS-232C and printer interfaces for external output of data. Refer to factory settings. 1. When in memory setup mode, press PRINT button and the display should show: 1. MEMORY PRINTOUT 2. INTERFACE SETUP 2. Press (2) to set up the interfaces. The display should show: INTERFACE SETUP RS-232C : 1. ON 2. OFF Refer to memory printout in #4 below. 3. Press (1) and ENTER to enter setup of RS- 232C. If RS-232C output is not required, press (2) and ENTER to quit RS-232C setup and proceed directly to step 7 for setup of printer. 4. The display will show: RS-232C SETUP BAUD RATE : 2400_ 1: : : : : : : 9600 The baud rate is pre-set at Select from the above list and press the number that stands for the required baud rate and press ENTER. The selected baud rate will show on the display. 29
30 RS-232 AND PRINTER 5. The display will then show: RS-232C SETUP DATA BIT LENGTH : 7_ The data bit length is pre-set at 7. Define the data bit length as required and press ENTER. 6. The display will then show: (0) : 0 RS-232C SETUP (1) : 1 PARITY : EVEN (2) : EVEN (3) : ODD The parity is pre-set at EVEN. Select from the above list and press the number that stands for the required parity and press the ENTER button. The selected parity will show on the display. 7. The display will then show: INTERFACE SETUP PRINTER: 1. ON 2. OFF The printer interface is pre-set at ON. Press ENTER to enter the setup. If printer output is not required, press (2) and ENTER buttons to quit. 8. The display will show: PRINTER TYPE SETUP 40/80 COLUMN: 80_ (1): 40 Column (2): 80 Column 30
31 RS-232 AND PRINTER The printer type is pre-set at 80 Column. Select from the above list and press the number that stands for the printer type to be used and press ENTER. The selected type will show on the display. 9. The display will show: PRINTOUT SETUP AUTO - LINE FEED : 1_ (0) auto 0 line feed (1) auto 1 line feed (2) auto 2 line feed (3) auto 3 line feed The line-feed is pre-set at 1 line. Select from the above list, press the required line-feed and press ENTER. The selected line-feed will show on the display. 10. The display will show: PRINTOUT SETUP I.D. NUMBER: YES (1) YES (2) NO Printout with I.D. number is pre-set to be required. Press ENTER button if so. Press (2) and ENTER buttons if not required. 11. The display will show: PRINTOUT SETUP WAVELENGTH: YES (1) YES (2) NO Printout with wavelength is pre-set to be required. Press ENTER button if so. Press (2) and ENTER buttons if not required. After all the above setups are completed, the system will return to memory setup mode. 31
32 External Output Settings Items Factory Setting RS-232C OFF Baud Rate 2400 Data Bit Length 7 Parity EVEN Printer ON Printer Type 80 Column Auto-Line Feed 1 I.D. Number YES I.D. Number 01 Wavelength YES Wavelength 440 nm Factor 1 32
33 Error Codes Note 1. Be sure to check the Blank solution or sample solution and WAVELENGTH first whenever an error message shows on the display. 2. After checking or correction, press 100%T/OA button to blank again. ERROR0 ERROR1 ERROR2 ERROR3 ERROR4 ERROR5 Instrument malfunction. Call for service Blank solution exceeds range. Absorbance of tested sample lower than that of blank solution. Absorbance of tested sample too high. Blank solution exceeds range. Instrument malfunction. Call for service. Note 1. Turn the power off and turn the power on to re-start the system. ERROR6 Filter Motor Error Instrument malfunction. Call for service. Filter Motor is not function ing. Wavel. Motor Error Wavelength Motor is not functioning. 2. If the display continues to show an error message, call for service. 33
34 One Year Limited Warranty Barnstead Thermolyne Corporation warrants that if a product manufactured by Barnstead Thermolyne and sold within the continental United States or Canada proves to be defective in material or construction, Barnstead Thermolyne will provide you, without charge, for a period of ninety (90) days, the labor, and a period of one (1) year, the parts, necessary to remedy any such defect. Outside the continental United States and Canada, the warranty provides, for one (1) year, the parts necessary to remedy any such defect. The warranty period shall commence either six (6) months following the date the product is sold by Barnstead Thermolyne or on the date it is purchased by the original retail consumer, whichever date occurs first. All warranty inspections and repairs must be performed by and parts obtained from an authorized Barnstead Thermolyne dealer or Barnstead Thermolyne. Heating elements, however, because of their susceptibility to overheating and contamination, must be returned to our factory, and if, upon inspection, it is concluded that failure is not due to excessive high temperature or contamination, warranty replacement will be provided by Barnstead Thermolyne. The name of the authorized Barnstead Thermolyne dealer nearest you may be obtained by calling or writing to: Barnstead Thermolyne P.O. Box Kerper Boulevard Dubuque, IA USA FAX: (319) Barnstead Thermolyne s sole obligation with respect to its product shall be to repair or replace the product. Under no circumstances shall it be liable for incidental or consequential damage. THE WARRANTY STATED HEREIN IS THE SOLE WARRANTY APPLICABLE TO Barnstead Thermolyne PRODUCTS. Barnstead Thermolyne EXPRESSLY DISCLAIMS ANY AND ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR USE. 34
35 35
36 Barnstead Thermolyne 2555 Kerper Blvd. P.O. Box 797 Dubuque, IA USA PHONE: FAX: Web Address: a subsidiary of 9001 ISO REGISTERED 36
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