Oils 7400 and Oils 7600 Homogenizing Autosampler

Transcription

1 Oils 7400 and Oils 7600 Homogenizing Autosampler Operator s Manual Manual Part Number Rev 1, October 2018

2 COPYRIGHT 2018 Teledyne Technologies Incorporated. All rights reserved Rev 1, October 2018, Teledyne CETAC Technologies authorizes its customers to reproduce, transmit, or store this document in its entirety, including this page, for the express purpose of installing, operating, or maintaining the product described herein. Teledyne CETAC Technologies Customer Service & Support Industrial Road Omaha, Nebraska 68144, USA Phone (800) (USA only) Phone (402) Fax (402) REVISIONS Teledyne CETAC Technologies strives to provide the scientific community with an unparalleled combination of effective technology and continuing value. Modular upgrades for existing instruments will continue to be a prime consideration as designs progress. Teledyne CETAC Technologies reserves the right to revise this document and improve products described herein at any time without notice or obligation. TRADEMARK ACKNOWLEDGEMENTS Windows is a registered trademark of Microsoft Corporation in the United States and other countries. PharMed and Tygon are registered trademarks of Saint-Gobain Performance Plastics. DuPont and Viton are trademarks or registered trademarks of E.I. du Pont de Nemours and Company. Superthane is a registered trademark of NewAge Industries, Inc. KIMWIPES is a registered trademark and KIMTECH SCIENCE is a trademark of Kimberly-Clark Worldwide, Inc All other marks are the property of their respective owners.

3 Contents 1 Introduction... 7 Overview... 7 About This Manual... 7 System Components... 8 Additional Equipment Required Sample Probe Optional Accessories Chemical Compatibility Non-Wetted Components Wetted Components Operator Qualifications Where to Go for More Information Installing the Autosampler Choosing a Location Space Requirements Work Surface Requirements Power Requirements Rinse Solution Requirements Liquid Waste Routing Requirements Unpacking the Autosampler Installing the ASX-7x00 Dashboard Software Inspecting the Z-Drive Connections Mounting the Tray Installing the Stirrer Installing and Adjusting the Sample Probe Connecting the Rinse Station Waste Rinse Solution Tubing Considerations Pumped Drain Arrangement Assembling and Placing the Sample Vial Racks Connecting to the Analytical Instrument s Sample Port Enclosure Connections Connecting the Autosampler to the Power Supply Connecting the Autosampler to the Host Computer

4 Contents Connecting a USB Cable Connecting a Serial Cable Verifying Installation Using the Autosampler Shutting Down the System Switching Between Coolant and Oil Samples Emptying the Drip Cup Flushing the Tubing Flushing the Autosampler Rinse Station and Tubing Changing Vial Spacing For Different Racks Choosing the Correct Probe/Stirrer Block Changing the Probe/Stirrer Block Using the Autosampler Utility Installing the ASX-7x00 Dashboard Software Starting the ASX-7x00 Dashboard Determining Which COM Port To Use Choosing the Autosampler Settings Maintaining the Autosampler Periodic Maintenance Tasks Checking for Leaks Emptying and Cleaning the Drip Cup Cleaning the Autosampler Daily External Cleaning Weekly Cleaning Cleaning the Lead Screws and Guide Rail Cleaning the Sample Probe Carbon Fiber (Coolants) Probes Stainless Steel (Oils) Probes Removing Tubing from Fittings Replacing Peristaltic Pump Tubing Replacing the Rinse Station Tubing Removing the Front Cover Opening the Chain Replacing the Battery Aligning the Autosampler Checking the Home Position Alignment Preparing for Long-Term Storage If the System Has Never Been Used If the System Has Been Used Troubleshooting Status LED Alignment Problems Power System Problems Communications Interface Problems RS-232 Serial Cable Problems USB Cable Problems Software Configuration Problems Contamination and Carryover Problems Rinse Solution / Reagent Problems

5 Contents System Configuration Problems Worn / Contaminated Component Problems Returning the Product to CETAC for Service Shipping the Product Product Warranty Statement Safety and Regulatory Information Characteristics Environmental Characteristics Electrical Characteristics Safety Notices Power Cord Set Requirements Power Cord Safety Maintenance Mains Disconnect Cleaning Instructions Mechanical Hazards Operating Environment Explanation of Caution and Warning Notices Electromagnetic Interference Explanation of Regulatory Marks

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7 1 Introduction Overview The Teledyne CETAC Oils 7400 and Oils 7600 homogenizing autosampler adds stirring to the capabilities of the ASX-7400 autosampler. The autosampler is designed to meet the demands of the oils testing industry. Two peristaltic pumps with discrete flow paths to a two-position rinse station allow easy switching between oils and coolants samples. About This Manual This manual covers the following Teledyne CETAC products: Oils rack homogenizing autosampler Oils rack homogenizing autosampler The Oils 7400 version is shown as an example throughout this manual. FIGURE 1-1 Oils 7400 Autosampler (Left) and Oils 7600 Autosampler (Right) 7

8 Chapter 1: Introduction System Components Cable Chain Power Indicator Lamp Z Drive Assembly Arm Autosampler Head Rinse Station Standards Vials Y Z X Sample Vial Racks Sample Tray Autosampler Base FIGURE 1-2 Oils 7400 Autosampler Front View Stirrer Motor Probe/Stirrer Block Sample Probe Stirring Paddle Drip Cup FIGURE 1-3 Z-Drive Assembly Right Side View 8

9 Chapter 1: Introduction Power Switch USB Port Serial Ports Ethernet Port Auxiliary Port Power Connector Stirrer Connector Peristaltic Pump (Oils) Peristaltic Pump (Coolants) Z-Drive Connector Rinse Station Fittings (Coolants) Rinse Station Fittings (Oils) FIGURE 1-4 Oils 7400 Autosampler Back View Power/Status Indicator Lamp. The LED is lit when the Oils 7400 homogenizing autosampler is connected to a power source and turned on. The color (blue or green) indicates the position of the rinse station (coolants or oils). Sample Tray. The sample tray holds the rinse station, sample racks, and standards vials in place. The tray also contains small spills. Sample Vial Racks. The Oils 7400 typically accommodates four sample vial racks; the Oils 7600 typically accommodates six racks. You can choose from different rack sizes. You can combine racks of different sizes as long as the host computer s software accepts the combination. Standards Vials. The autosampler has positions for ten standards vials. The standards vials are 50-milliliter conical centrifuge vials with caps. Rinse Station. The rinse station washes the sample probe and stirring paddle. The dual rinse station can be easily switched between two positions for different solvents. Arm. The arm moves the sample probe horizontally. Z-Drive. The Z-drive moves the sample probe and stirring paddle vertically. The Z-drive assembly includes a Y-axis slider block and guide plate. The Z-drive attaches onto the autosampler arm. Drip Cup. The drip cup is automatically positioned below the sample probe and stirring paddle when the Z-drive is raised. This prevents liquid from dripping into sample and standard vials. Peristaltic pump(s). One or two two-channel peristaltic pumps move the rinse solution from the rinse source through the rinse station. Power connector. Power from the desktop transformer comes into one connector (either connector may be used as the input). USB Port and Serial Ports. The USB or serial port connects the autosampler with the analytical instrument s host computer. If you use the serial ports, COM1 connects the autosampler with the analytical 9

10 Chapter 1: Introduction instrument s host computer and COM2 connects the autosampler to other external devices. Ethernet Port. The Ethernet port can be used to interface the autosampler with the host computer. This port and the associated LEDs are disabled by default. Z-Drive Connector. The Z-drive contains an integral motor and lead screw, and is powered and controlled through this connector on the back of the autosampler. Stirrer Connector. Provides power to the stirrer on the Z-drive assembly. Auxiliary Port. An input/output connector allows single direction or bidirectional communication with other instrumentation. Power Supply. A desktop "brick" transformer powers the autosampler. Sample Probe Kit. The kit includes the sample probe and sample transfer tubing. Stirring Paddle. The paddle stirs one sample while the sample probe aspirates the previously stirred sample. Probe/Stirrer Block. This block positions the sample probe and stirring paddle. The block determines the spacing between the probe and stirrer; this spacing must match the vial-to-vial spacing of the rack which is in use. The following standard components are also shipped with the Oils 7400 homogenizing autosampler: Tubing Kit. USB Cable and serial cable. CD. The CD contains, if applicable: ASX-7x00 Dashboard software USB device drivers Autosampler alignment files Manuals Other application-specific information Supplied components depend on the application and the exact version of the product. See the packing list in the shipping container to see exactly which components are supplied. Additional Equipment Required In addition to the provided equipment, you will need the following items: One or two rinse solvent bottles (separate bottles for oils and for coolants, as appropriate) One or two liquid waste containers for the rinse solvent, 10 liters or larger (separate containers for the waste oils solvent and for the waste coolants solvent) Host computer with a free USB or serial connector 10

11 Chapter 1: Introduction Sample Probe It is important to choose a sample probe with the appropriate inside diameter for your application. The probe diameter is indicated by the color of the band on the sample tubing just above the probe. Steel probes are available for oils applications; coated carbon fiber probes are available for coolant applications. See the Accessories and Parts Catalog for more information on the probes which are available. Band Color Indicates Probe Diameter FIGURE mm Carbon Fiber Sample Probe Optional Accessories You may also wish to use: Enclosure. The autosampler and all open vessels stay within the enclosure to protect samples from contamination and to contain fumes. Teledyne CETAC offers several enclosures to fit different sizes of autosamplers and different ventilation requirements. ASXPRESS PLUS Rapid Sample Introduction System. 6-port valve system which enables rapid sample loading and wash out. Sample Racks. You can order a variety of sample racks to meet different needs. See the Accessories and Parts Catalog for more information on the available racks. Note that you will need a probe/stirrer block with a spacing which matches the vial-to-vial spacing of the racks. 11

12 Chapter 1: Introduction Chemical Compatibility Non-Wetted Components Autosampler components are made of corrosion-resistant stainless steel alloys or anodized aluminum. The autosampler head and base are made from a high-strength aluminum alloy that is treated with a RoHS-compliant chemfilm and finished with an epoxy powder coating. Do not expose the autosampler to acids (except for cleaning purposes, as specified in this manual) or acid vapors. The autosampler head is not sealed against liquid or vapor intrusion. Longterm exposure of the internal components to corrosive materials may cause damage. Wetted Components The Oils 7400 system is intended for use with sample matrices with the following characteristics: low-acid aqueous solutions (up to 1% HNO3 or HCl) low-viscosity oils low to high dissolved solids The system is not suitable for high viscosity samples. NOTICE Avoid use with chemicals which are likely to precipitate or form deposits on the internal surfaces, such as silver chloride. Samples which contain AgCl may contaminate the internal surfaces and require the replacement of the tubing. Wetted components are made of Polyetherimide (PEI) and Polytetrafluoroethylene (PTFE). When these inert, non-metallic materials are used at temperatures less than 135 C, they can withstand repeated exposure to the following substances: Predominantly aqueous nonacidic solutions Predominantly aqueous solutions of strong bases (less than 10%) Common organic solvents such as acetone, alcohols, ethyl acetate, Methylethylketone (MEK), petroleum oils and derived fuels, tetrachloroethylene, toluene, kerosene and xylene. WARNING CHEMICAL HAZARD Do not use the autosampler with substances which could pose a hazard of serious injury to the operator if spilled or injected, such as formic acid. 12

13 Chapter 1: Introduction NOTICE DAMAGE FROM CHEMICAL EXPOSURE Prolonged or repeated exposure to temperatures greater than 135 C and to the following substances can cause failure of the wetted components: Acids (except for cleaning purposes, as specified in this manual). Solutions of concentrated bases (greater than 10% potassium, ammonium, or sodium hydroxides). Partially halogenated hydrocarbons or extremely aggressive organic solvents (chloroform, methylene dichloride, 1,1,2-trichloroethane). Operator Qualifications The primary audience for this manual consists of analytical chemists and lab technicians. To use this product, you should have a basic knowledge of chemistry, a basic knowledge of electronic sampling equipment, at least a beginning level of computer experience, and working knowledge of the analytical instrument used with the autosampler. WARNING CHEMICAL INJURY HAZARD The Oils 7400 system is for use only by qualified operators who have been trained in safe laboratory practices. Make sure you know the hazards associated with all of the chemicals you are using, and take the appropriate precautions. Exposure to laboratory chemicals may result in serious injury. 13

14 Chapter 1: Introduction Where to Go for More Information In addition to this manual, you can refer to the following resources: New versions of this manual, along with application and technical notes, are available on CETAC s Web site: CETAC Autosampler Safety Guide provides basic safety-related information in a variety of languages. Oils 7400 Spare Parts and Accessories Catalog, available on the CETAC Web site. Oils 7400 Quick Installation Guide provides abbreviated instructions for installing the autosampler for a typical application. Online help within the ASX-7x00 Dashboard software. Teledyne CETAC Technologies Customer Service and Support: Phone: 1 (800) (USA only) 1 (402) Fax: 1 (402) cetacservice@teledyne.com 14

15 2 Installing the Autosampler No tools are required to install the autosampler. You can remove thumbscrews with tools if necessary, but do not tighten them with anything other than your fingers. WARNING Ensure the power switch is off and the power cord is unplugged before proceeding with installation. If the power is left on, motors may move unexpectedly and cause injury. Choosing a Location Position the Oils 7400 homogenizing autosampler near the ICP. Rinse bottles may be placed beside, behind, or under the autosampler. Space Requirements The recommended footprint for the autosampler is shown in the following tables. Space requirements for specific configurations may be different, depending on the configuration and accessories. Autosampler Dimensions Recommended Space Including Cables/Tubing Height 49 cm (19.3") 54 cm (21.3") Width 57 cm (22.4") 57 cm (22.4") Depth 54 cm (21.3") 59 cm (23.3") Weight 23 kg (50 lbs) TABLE 2-1: Physical Characteristics Oils

16 Chapter 2: Installing the Autosampler Autosampler Dimensions Recommended Space Including Cables/Tubing Height 49 cm (19.2") 54 cm (21.2") Width 81.0 cm (31.9") 81.0 cm (31.9") Depth 54 cm (21.3") 59 cm (23.2") Weight 35 kg (77 lbs) TABLE 2-2: Physical Characteristics Oils 7600 The weight shown is that of the autosampler itself, without the desktop power supply, tray, vials, sample probe, or tubing. You will need easy access to the back of the autosampler to reach the power switch. Allow at least 5 cm behind the autosampler for cable egress, ventilation, and access to the power switch. Always position the equipment so that it is easy to disconnect the power cord. Work Surface Requirements The autosampler must be placed on a sturdy countertop, cart, or table. Due to its weight, do not place the autosampler on a folding table. If the work surface is allowed to shake or wobble, the autosampler may walk across the surface or vibrate excessively. NOTICE PLACE THE AUTOSAMPLER ON A STURDY SURFACE If the work surface is allowed to shake or wobble, liquids may spill or data quality may be affected. Power Requirements The autosampler is powered by an external desktop "brick" power supply. Place the autosampler within 1.2 meters of a power outlet. WARNING SHOCK AND FIRE HAZARD Use only the power supply which is provided with the autosampler. The power supply must be plugged into an outlet which has a protective ground connection. The power supply must be connected to an AC power source that will not apply more than 240 VAC between the supply conductors and ground. A protective ground connection is required for safe operation. Ensure that you position the autosampler so that the plug is easily accessible (is not blocked) and can be quickly disconnected if needed. In case of hazard, the autosampler should be disconnected from the power source. The power supply socket is on the back of the autosampler below the power switch. Connect the power supply to the autosampler first and then connect a line cord to the power supply. Do not plug in the power supply until ready to operate the autosampler. 16

17 Chapter 2: Installing the Autosampler Rinse Solution Requirements The sample matrix and analytical method will determine which rinse agent should be used. For most coolant applications, deionized water is used as the rinse agent. For most oils applications, kerosene is used as the rinse agent. Place the rinse agent source within two meters of the autosampler. Liquid Waste Routing Requirements Ensure that there is a liquid waste receptacle within two meters of the autosampler. The waste receptacle inlet should be 30 to 60 centimeters lower than the autosampler rinse station outlet and set up so that the rinse drain tubing drops directly into the waste receptacle with no coiling and without being submerged below the liquid level of the waste receptacle. NOTICE SUBMERGED DRAIN TUBING MAY CAUSE EQUIPMENT DAMAGE Ensure that the rinse drain tubing is not submerged in the waste liquid. If the tubing is submerged, waste liquid can back up in the rinse station, flooding the sample tray. Unpacking the Autosampler CAUTION LIFTING HAZARD Two people are required to lift the autosampler. Lifting should be done with a person situated on either side of the instrument. Lifting without assistance may cause injury. Inspect external packaging upon receipt for signs of shipping damage. Inspect all items during unpacking and notify the carrier immediately of any concealed damage. Check for kinked or bent tubing. If the system is shipped or removed from storage during cold weather, allow the packaged equipment to equilibrate to room temperature before opening and exposing to warm, humid air. It is usually sufficient to provide four to eight hours for this purpose. NOTICE EQUIPMENT DAMAGE FROM CONDENSATION If condensation forms on or inside the autosampler, allow it to dry thoroughly before connecting it to a power source and operating it. Using the device before it is dry may cause equipment damage. Remove the packing checklist from the shipping container, and check off items against it. Leave accessories in the packing until you are ready to install them. 17

18 Chapter 2: Installing the Autosampler NOTE Keep the original packaging for use in case the product ever needs to be returned or shipped to another location. Installing the ASX-7x00 Dashboard Software 1 On the included CD, double-click the ASX Dashboard installation file and follow the prompts to complete the installation. You do not need to run the software yet. The installation includes a USB driver which Windows should automatically find when you connect the autosampler to the PC. Inspecting the Z-Drive Connections 1 Check the Z-drive connector and stirrer connector to make sure that the cables have not come loose during shipment. Stirrer Connector Z-Drive Connector FIGURE 2-1 Z-Drive Connections Mounting the Tray 1 Place the tray on the base of the autosampler. Make sure the locating pins on the tray fully seat into the locating holes in the base. 18

19 Chapter 2: Installing the Autosampler Installing the Stirrer When the autosampler is used for oils, install the stirrer paddle. When the autosampler is used for coolants, the stirrer paddle should be removed (or replaced with the optional coolants stirrer paddle). 1 Locate the oils stirrer paddle. Oils and coolants require the use of different paddles. FIGURE 2-2 Stirrer Paddle for Oils FIGURE 2-3 Coated Stirrer Paddle for Coolants (Note the Band) 2 Press the stirrer paddle into the hole on the bottom of the stirrer motor. FIGURE 2-4 Inserting the Stirrer Paddle 3 Place the stirrer motor into the larger hole in the probe/stirrer block on the Z- drive. FIGURE 2-5 Placing the Stirrer Motor 19

20 Chapter 2: Installing the Autosampler 4 Tighten the two thumbscrews. FIGURE 2-6 Securing the Stirrer Installing and Adjusting the Sample Probe 1 Choose the appropriate probe for your application. 2 Locate the two thumbscrews which are supplied with the probe. 3 Guide the probe straight down through the smaller hole in the Z-drive. 4 Adjust the height of the probe so that the tip of the probe is level with the tip of the stirrer paddle. FIGURE 2-7 Probe Level With Stirrer Paddle 20

21 Chapter 2: Installing the Autosampler 5 Tighten the thumbscrews. FIGURE 2-8 Installing the Probe 6 Check the position of the sample tubing. Always position the sample transfer tubing so that it does not pull on the probe. The tubing should naturally curve away from the probe so that it won't rub or get caught. Be sure to check that the tubing will not be stretched and will not snag on an obstacle when the probe is moved to the far corner sample positions. 7 Optional: If you are installing a coolants probe, don appropriate personal protective equipment, then moisten a Kimwipes wiper with 10% HNO3 and wipe the probe to remove any aqueous or inorganic contamination from the surface. Then moisten a new Kimwipes wiper with a chromatography grade solvent such as n-propanol, isopropanol, or hexane and wipe the probe again to remove any oil. Let the probe air dry. Be careful not to touch the probe without gloves residue from handling the probe can cause the surface tension properties of the probe surface to change. Periodic cleaning may be necessary depending on the application. If you are installing an oils probe, no initial cleaning is necessary. Connecting the Rinse Station The rinse station is mounted at the left end of the autosampler. For coolants samples, the rinse solution is typically deionized water or a dilute acid rinse such as 1% HNO3. For oils samples, the rinse solution is typically a solution from the kerosene family. The rinse solution flows from the sample probe reservoir to the drain (rinse out) reservoir within the rinse station. The waste rinse solution is then drained from the rinse station. The inlet of the rinse station is at the bottom of the rinse station. The rinse solution flows from the bottom to the top of the sample probe reservoir. Upflow rinsing is the most effective method for decontaminating the sample probe tube between samples. Reversing the connections will reverse the flow direction, resulting in cross-contamination and unsatisfactory performance. 21

22 Chapter 2: Installing the Autosampler Waste Rinse Solution In most cases, rinse solution will be recycled by returning it to the rinse solution bottle. If necessary, rinse solution can be pulled from a fresh bottle and used solution drained to a waste container. Remember to label the waste container according to your laboratory policy and local regulations. CAUTION Ensure that the tubing outlet is placed so that it will remain above the surface of the liquid in the waste container. If the end of the tube is immersed, the waste solution might back up and overflow. Tubing Considerations Verify that the supplied tubing material is compatible with the rinse solution you are using. Clear Superthane tubing is supplied (except for the peristaltic pump tubing cartridge). There are many different types of tubing that can be used for the rinse station. Clear Tygon, Viton, Tygon Fuel and Lube (yellow), and PharMed (opaque) may be used depending on the application and user preference. Refer to the Oils 7400 Spare Parts and Accessories Catalog or contact CETAC if you need different tubing material. The fittings on the rinse station and on the peristaltic pump use ⅛ inch (3.2mm) ID tubing. Carefully press the tubing straight on to the fittings to avoid breaking the fittings. If you find it difficult to get a good connection to the rinse station, remove the rinse station and press the tubing firmly so that it completely covers the barb of the fitting. It helps to use your other hand to apply counterpressure. Pumped Drain Arrangement In a pumped drain arrangement, one channel of the peristaltic pump is used to supply rinse solution to the rinse station, while the other channel of the pump drains the waste rinse solution. Normally, the peristaltic pump channel which is closest to the autosampler chassis is used to pull liquid out of the rinse station and the channel which is farther from the autosampler is used to feed in the fresh rinse solution. 22

23 Chapter 2: Installing the Autosampler Figure 2-9 Pumped Drain Tubing Connections To connect the rinse station using the pumped drain, complete the following steps. Repeat this procedure for both the Oils and Coolants peristaltic pump. 1 Connect up to 1.8 meters of tubing from the outlet fitting of the pump (the lower fitting of the channel closest to the autosampler) to the waste container. Figure 2-10 Connecting the Waste Line Ensure that the tubing outlet is placed so that it will remain above the surface of the liquid in the waste container. If the end of the tube is immersed, the waste solution might back up and overflow. 23

24 Chapter 2: Installing the Autosampler 2 Cut two pieces of tubing to go between the peristaltic pump and the rinse station; ensure that the tubing is long enough to reach without any sharp bends or kinks. For the Oils side, the tubing should be about 25 cm (10 inches) long; for the Coolants side, it should be about 28 cm (12 inches) long. Figure 2-11 Cutting Tubing 3 Connect one of the pieces of tubing from step 2 to the input (top) of the pump channel. 4 Connect the end of the tubing to the outlet (upper) fitting of the rinse station. The Coolants peristaltic pump should be connected to the blue side of the rinse station and the Oils pump should be connected to the green side of the rinse station. FIGURE 2-12 Connecting the Rinse Station Drain (Oils Side Is Shown) 5 Connect the second piece of tubing from step 2 to the fitting at the bottom of the pump, farthest from the back of the autosampler. 6 Connect the other end of this second piece of tubing to the bottom fitting of the rinse station. 24

25 Chapter 2: Installing the Autosampler FIGURE 2-13 Connecting the Rinse Station Inlet (Oils Side Is Shown) 7 Use an appropriate length of the tubing to connect the rinse solution source to the fitting at the top of this channel. From Rinse Solution Source FIGURE 2-14 To Waste Container Completed Rinse Station Tubing for the Oils Side 25

26 Chapter 2: Installing the Autosampler Gravity Drain Arrangement For a gravity drain arrangement, connect the rinse input tubing as shown for the pumped drain. Connect a length of tubing from the drain fitting of the rinse station (the upper fitting) directly to the waste container. FIGURE 2-15 Gravity Drain Tubing Connections CAUTION Ensure that the tubing outlet is placed so that it will remain above the surface of the liquid in the waste container. If the end of the tube is immersed, the waste solution might back up and overflow. 26

27 Chapter 2: Installing the Autosampler Assembling and Placing the Sample Vial Racks Some styles of sample vial racks are shipped unassembled. You can easily assemble them without using tools. WARNING PUNCTURE HAZARD Never attempt to load, unload or reposition the sample vial rack or sample vial while the autosampler is operating. The sample probe may move unexpectedly and cause an injury. 1 Before loading or unloading any sample vial racks on the sample tray, park the sampling arm and probe in the home position. The home position is the initial position at power up. 2 If necessary, assemble the racks as shown in the instructions included with each rack. 3 Place the first sample vial rack at the extreme left-hand side of the sample tray. Place the next sample vial rack to the right of the first rack, and so forth. Connecting to an ASXPRESS PLUS System For dual oils and coolants operation, use two valve/pump modules. Use a white valve/pump module for coolants, and a gray one for oils. See the ASXPRESS PLUS Rapid Sample Introduction System Installation Guide for more information. If you are using an existing ASXPRESS PLUS system (not purchased with the autosampler), then you may also need to: 1 Install the ASX Dashboard from the Oils CD. Do not use the standalone Xpress Configuration Tool which came with the ASXPRESS PLUS. For information on using the ASXPRESS PLUS with the ASX Dashboard, download the latest ASXPRESS PLUS Rapid Sample Introduction System Operator s Manual from 2 Upgrade the ASXPRESS PLUS firmware to version 2.63 or greater. 3 Set the ASXPRESS PLUS personality to the appropriate value. 27

28 Chapter 2: Installing the Autosampler Connecting to the Analytical Instrument s Sample Port You can connect the autosampler directly to a sample introduction peristaltic pump and then to any sample introduction device, such as the CETAC U5000AT + or U6000AT + Ultrasonic Nebulizer. One end of the sample transfer tubing is preconnected to the sample probe. The transfer tubing should be long enough so that it there will be no strain on the tubing connectors even when the sample probe is at the position farthest from the analytical instrument. On the other hand, keep the sample transfer tubing as short as possible. This will: Minimize washout time Minimize sample carryover Reduce the chance of tangled tubing 1 Determine the length of the sample transfer tubing you need, and cut it to size. 2 Connect the free end of the sample transfer tubing to the inlet of the analytical instrument s peristaltic pump. Enclosure Connections If an enclosure is present, connect the trimmed tubing from the sample probe to the inside bulkhead using the nut and ferrule provided with the enclosure. Then attach tubing from the outside bulkhead to the input of the analytical instrument. Connecting the Autosampler to the Power Supply The autosampler is powered by the supplied external desktop "brick" power supply. Ensure that you position the autosampler so that the location where the power supply cord plugs into it is easily accessible (is not blocked) and it can be quickly disconnected if needed. In case of hazard, the system should be disconnected from the power source. 1 Turn the power switch on the autosampler OFF. FIGURE 2-16 Power Switch 2 Check the plug on the power cord to verify that it is of the correct type for your country. 28

29 Chapter 2: Installing the Autosampler 3 Plug the power cord into a power outlet. 4 Plug the power cord into the power supply. To POWER Connector on Autosampler Power Cord to Grounded AC Power Outlet FIGURE 2-17 Power Supply 5 Plug the power supply into the 24 V connector on the autosampler. 6 Turn the power switch on the autosampler ON. It is important to use the appropriate power cord for your country. See Power requirements on page 16 and page 62. Power Cord Set Requirements on page 64. WARNING SHOCK AND FIRE HAZARD Use only the power supply which is provided with the autosampler. The power supply must be plugged into an outlet which has a protective ground connection. Connecting the Autosampler to the Host Computer Software on the host computer controls both the analytical instrument and the autosampler. You cannot operate the autosampler until you establish a communication interface between the autosampler and the host computer. It is through this interface that the host computer directs the operation of the autosampler. The autosampler supports the following communication interfaces: The USB interface is the standard configuration. A virtual COM port is created so that the connection looks like a standard RS-232 serial port to the host PC software. The serial (RS-232) interface is simple and reliable, but the host computer may lack a free serial port. NOTE: Use either a USB cable or a serial cable, but not both. The serial port will not function when a USB cable is connected. 29

30 Chapter 2: Installing the Autosampler Connecting a USB Cable You will need an A-B USB cable. FIGURE 2-18 USB Cable 1 Power on both the computer and the autosampler. 2 Plug one end of the cable into the host computer's USB port and the other end into the autosampler s USB port. Windows will automatically find the driver which was installed with the ASX- 7x00 Dashboard software. 3 When driver installation is complete, make a note of which COM port number was assigned. The COM port number may be displayed in a bubble in the lower right corner of the screen. Connecting a Serial Cable You will need a serial cable equipped with two male DB-9 connectors. 1 Plug one end of the cable into the host computer s serial (COM) port. If the computer has more than one serial port, make sure to use the same port which is selected in the instrument control software. 2 Finger-tighten both screws of the connector. 3 Connect the other end of the cable to the autosampler s COM1 port. 4 Finger-tighten both screws of the connector. NOTES Do not use a USB-to-serial adapter. A USB connection is strongly recommended for the ASX-7x00 Dashboard software. The Firmware Updater utility requires a USB connection. If a host computer serial port with a DB-9F, a DB-25M, or a DB-25F connector (9 pin D-subminiature receptacle or 25 pin D-subminiature plug or receptacle) must be used, use an adapter to convert the serial port to a DB-9M. Do not use a null modem adapter. Ensure you are connecting the serial cable to the COM1 port on the autosampler. Connecting the adapter to the COM2 port will cause a malfunction. 30

31 Chapter 2: Installing the Autosampler When interconnecting any computing devices, keep the communications cables away from sources of electromagnetic or radio frequency (RF) interference, such as electric motors, transformers, fluorescent light ballasts, or RF energy sources. Limit cable runs for RS-232C to less than 16 meters. If these conditions cannot be satisfied, use low-impedance, fully shielded cables to provide satisfactory operation. The cables are available from many sources, but you will need to specify the correct mating connectors and straightthrough (DTE-DCE) wiring. Verifying Installation Once installation of the autosampler is complete, it is important to verify that you have installed it correctly. Attempting to use it before ensuring that it is installed correctly may result in damage to the autosampler. Ensure that the communications interface between the autosampler and the host computer is working. Ensure that the sample probe functions properly. Ensure that the rinse solution drains properly. Ensure that there are no obstacles which could interfere with the free movement of the autosampler arm, probe and stirrer, or sample tubing. This can be done using your ICP-MS or other instrument control software, or with the IQ/OQ utility which is part of the provided ASX-7x00 Dashboard software (see page 39). Qualification testing is required for laboratories in some industries with Good Laboratory Practices (GLP) or Good Manufacturing Practices (GMP) requirements. If your laboratory requires installation qualification (IQ) and operational qualification (OQ), refer to the IQ/OQ guide available on the CD or at 31

32 Chapter 2: Installing the Autosampler This page is intentionally blank. 32

33 3 Using the Autosampler Starting the System WARNING PINCH HAZARD Keep fingers, hair, and loose clothing away from the moving parts of the autosampler. To start the autosampler: 1 Ensure that all tubing is properly connected. 2 Visually inspect the sample area for misplaced vials and obstructions. Also make sure that any caps have been removed from the vials. 3 Turn the autosampler power switch on. The LED power indicator lights up when the power is on. Shutting Down the System To shut down the autosampler, complete the following steps: 1 Turn off the autosampler power switch. 2 If you will be performing maintenance, unplug the power cord either at the power supply or at the wall outlet. 33

34 Chapter 3: Using the Autosampler Switching Between Coolant and Oil Samples 1 Move the rinse station to the appropriate position. The status LED on the front of the autosampler will show green for the oils position or blue for the coolants position. FIGURE 3-1 Rinse station in coolants (left) and oils (right) position. 2 Moving the rinse station will automatically switch to the appropriate peristaltic pump on the back of the autosampler. Visually verify that the tubing is directed to the correct rinse supply and waste containers. 3 Replace the sample probe with the appropriate version. 4 Replace the stirring paddle with the appropriate version. For oils, use the oils paddle. For coolants, remove the paddle or use the optional coolants paddle (identified by a black band). 5 If using a Teledyne CETAC ASXPRESS PLUS Rapid Sample Introduction System, use a second valve/pump module which has been set up for oils or coolants. See the ASXPRESS PLUS. Operator s Manual for more information. 6 Replace or clean any other components as appropriate. For example, you might need to replace a standard addition tee or the ICP-MS nebulizer. 34

35 Chapter 3: Using the Autosampler Emptying the Drip Cup CAUTION Chemical Burn Hazard Wear gloves and other personal protective equipment, as appropriate. If you do not wear gloves, skin contact with the chemicals in the drip cup is likely, and chemical burns could occur. 1 Turn the autosampler off. 2 Loosen the thumbscrews and move the stirrer and probe up a few inches, then tighten the thumbscrews. 3 Push the Z-drive to its top position so that the drip cup swings out. 4 Press up on the bottom of the drip cup to lift it out. 5 Empty the drip cup, clean it and then return it to the cup holder. Flushing the Tubing Generally, you can operate the autosampler without flushing the tubing. However, you need to flush the tubing and rinse station under two circumstances: During initial startup of the autosampler after installation. After the use of strong bases, acids, or organic solvents as rinse agents. Flushing during initial startup of the autosampler removes any contaminants that could cause interference during sample analysis. Flushing after using strong rinse agents prevents degradation and failure of the tubing. Flushing the Autosampler Rinse Station and Tubing To flush the autosampler rinse station and flow path, complete the following steps: 1 Place the sample probe into a container of deionized water. NOTE If you are flushing the rinse system during initial startup, first use a 2% nitric acid solution as the rinse agent, followed by deionized water. 2 Run the rinse solution through the rinse station and flow path for 5 to 10 minutes. 35

36 Chapter 3: Using the Autosampler Changing Vial Spacing For Different Racks When the autosampler is shipped, it is set up for a particular kind of rack. If you change to another kind of rack, you will probably need to make a few changes: 1 Obtain and install a probe/stirrer block with the appropriate spacing. See the information in the next section. See Changing the Probe/Stirrer Block on page Configure the autosampler firmware for the sample coordinates with the new racks. To do this, use the ASX-7x00 Dashboard software. 3 Configure the host instrument software for the sample coordinates with the new racks. Depending on the instrument and software you are using, you may need to choose the kind of rack you are using from a menu or load a rack file. 4 Check the Z-axis travel. Use the manual controls in your instrument control software to make sure that the probe and stirring paddle descend to the correct depth in the sample vials. Choosing the Correct Probe/Stirrer Block NOTICE Always use the correct probe/stirrer block for your sample racks. Using an incorrect probe/stirrer block will cause the probe and stirring paddle to collide with the vial or rack, which can cause equipment damage. Refer to the Teledyne CETAC Oils 7400 Accessories and Parts Catalog for more information. CETAC Part Number Spacing (inches) Compatible Racks /80 position and Janus /96 position Gilson TABLE 3: Probe/Stirrer Blocks Changing the Probe/Stirrer Block 1 Remove the sample racks and standards vials from the autosampler. 2 Move the Z-drive assembly toward the center of the autosampler. This will help ensure that any dropped screws will be easy to retrieve. 36

37 Chapter 3: Using the Autosampler 3 Remove the four screws which attach the probe/stirrer block bracket to the Z- drive. FIGURE 3-2 Detaching the bracket from the Z-drive. 4 Flip the probe/stirrer block to expose four more screws; remove those screws. The bracket will stay attached to the Z-drive assembly. FIGURE 3-3 Removing the probe/stirrer block from the bracket. 37

38 Chapter 3: Using the Autosampler 5 Attach the new probe/stirrer block to the bracket then attach the bracket to the Z-drive. Note that the probe/stirrer block has four holes for the screws and also four holes for the thumbscrews which hold the stirrer paddle and probe in place. FIGURE 3-4 Orientation of probe/stirrer block on the bracket. 38

39 4 Using the Autosampler Utility Once the autosampler is installed, you need to select the appropriate settings in several places: Movement and pump speeds in the ASX-7x00 Dashboard software. Method-specific settings in the software which controls your analytical instrument. The ASX-7x00 Dashboard software is supplied on the included CD. Other software on the CD includes the C-Term program for communicating directly with the autosampler and a USB driver. The ASX-7x00 Dashboard software includes the following utilities: Autosampler IQ-OQ assists with qualification (IQ and OQ) by performing a set of predefined movements. See the IQ/OQ Guide on the CD for more information. Log Extractor saves error codes and operating history information so that it can be sent to Teledyne CETAC support. Firmware Updater provides a way to program the autosampler with new firmware. Changing the firmware can give the autosampler a new personality to make it compatible with certain analytical instruments. Note that you should connect the autosampler with a USB cable, not an RS-232 serial cable, to update firmware. Autosampler Config lets you set the X-Y-Z motion speed, peristaltic pump speed, and stirrer settings such as the stirring speed and Smart Stir. Xpress Config lets you configure the operation of a Teledyne CETAC ASXPRESS PLUS Rapid Sample Introduction System. This chapter provides instructions for installing and starting the ASX-7x00 Dashboard. The Help button within each utility provides additional information. 39

40 Chapter 4: Using the Autosampler Utility Installing the ASX-7x00 Dashboard Software 1 On the included CD, find the DashboardInstall folder. 2 Double-click setup.exe. 3 Follow the prompts to complete the installation. Starting the ASX-7x00 Dashboard 1 Check that the autosampler is connected to the PC using a USB cable. NOTE A USB connection is strongly recommended for the ASX-7x00 Dashboard software. The Firmware Updater utility requires a USB connection. The other utilities can use a direct serial connection, if necessary. Do not use a USB-toserial converter. Remember that the USB connection will appear as a virtual COM port on the PC. 2 Check that the autosampler is turned on. 3 Open the tool. FIGURE 4-1 ASX-7x00 Dashboard Icon 4 Click on the utility you wish to run. 40

41 Chapter 4: Using the Autosampler Utility FIGURE 4-2 ASX-7x00 Dashboard Main Screen 5 For information about how to use any of the utilities, click. Determining Which COM Port To Use Any software you use to communicate with the autosampler will need to know which COM port is connected to the autosampler. If you are using a USB connection, the COM port numbers were assigned by the USB driver when you connected the USB cables for the first time. Ideally, the port numbers were written down at that time. If you do not know which port number was assigned to the autosampler, you can use the Windows Device Manager to distinguish the port numbers: 1 Connect the USB cable between the computer and the autosampler, and turn it on. 2 Open the Windows Device Manager. The quickest way to do this is to click the Windows Start button and enter device manager in the search field. 3 In Device Manager, click Ports (COM & LPT). You will see a list of all of the COM port numbers which have been assigned. 41

42 Chapter 4: Using the Autosampler Utility FIGURE 4-3 COM Port Assigned to a USB Port in Device Manager 4 Unplug the USB cable from the autosampler. 5 Note which COM port disappears from the list. This is the port number of the autosampler. 6 Plug the USB cable back in to restore communication. Choosing the Autosampler Settings The settings you choose depend on your method and on the characteristics of the sample (such as viscosity). Some settings change the balance between speed and accuracy. Some experimentation will be required during your method development so that you can be confident that the settings are best for the needs of your laboratory. Contact Teledyne CETAC Service and Support (cetacservice@teledyne.com) if you have questions or need assistance. 42

43 5 Maintaining the Autosampler Routine maintenance of the autosampler consists of daily and weekly cleaning of specific autosampler components. Routine maintenance also includes checking for leaks or other damage. Additional periodic maintenance tasks may be required, such as replacement of tubing. NOTICE Discharge static buildup and ground to the autosampler base or cabinet before performing any maintenance. Do not touch or short-circuit the contacts on the communication ports. Periodic Maintenance Tasks The recommended maintenance intervals in TABLE 4 are based on typical use. You should adjust the intervals depending on the samples and solvents you are using, and depending on the lower detection limit of your methods. Interval Task Daily or Weekly Empty the drip cup Check for leaks External cleaning 6 Months Replace peristaltic pump tubing Replace sample probe Yearly Replace rinse and drain tubing TABLE 4: Recommended Maintenance Intervals 43

44 Chapter 5: Maintaining the Autosampler Checking for Leaks You should regularly check all tubing for leaks. Visually inspect the sample probe, peristaltic pump tubing, and rinse station tubing for leaks or signs of deterioration. If you detect a leak or other damage to a component, you must replace it. For more information, see the appropriate section in this chapter. Emptying and Cleaning the Drip Cup If liquid has accumulated in the drip cup: 1 Remove any vials from below the area of the Z-drive assembly. 2 Raise the Z-drive assembly to its highest position. 3 Remove the stirrer (you can leave it attached to the stirrer motor) and the probe from the Z-drive. FIGURE 5-1 Exposing the drip cup. 44

45 Chapter 5: Maintaining the Autosampler 4 Press the drip cup up from the bottom. FIGURE 5-2 Removing the drip cup. 5 Empty the cup and wipe it off. FIGURE 5-3 Wiping the drip cup. 6 Replace the drip cup, probe, and stirrer. TIP Sometimes it can help to leave a piece of absorbent mat or folded paper towel in the bottom of the drip cup to absorb liquid. NOTICE Do not clean the drip cup with sodium hydroxide, which can damage the cup (it is made of PET). It is not necessary to completely clean the drip cup, so solvents are not normally needed to clean it. Cleaning the Autosampler Cleaning the autosampler is the primary maintenance task you perform. Failure to do so regularly causes increased wear and reduces the autosampler s life. You must clean the autosampler both daily and weekly to prevent damage and extend its life. It is especially important to clean up spills and remove contaminants, such as abrasives, from the autosampler s moving parts. It may also be necessary to chemically neutralize spills. The following sections explain daily and weekly cleaning procedures. 45

46 Chapter 5: Maintaining the Autosampler NOTICE Do not clean with carbon tetrachloride. NOTICE Do not allow the cleaning agent to come into contact with the lead screws. Never lubricate the lead screws. Daily External Cleaning Use of the autosampler often results in spills on autosampler components such as the sample tray. Good maintenance requires that you clean the autosampler daily. To do so, complete the following steps: 1 Shut down and unplug the autosampler. For information about shutting down the autosampler, see page Wipe the sample tray, autosampler cabinet, and autosampler arm using a towel dampened with a lab-grade cleaning agent. NOTICE Do not allow the cleaning agent to come into contact with the lead screws. Also, never lubricate either of the two lead screws. 3 Repeat step two, using a towel dampened with clear water. This process removes any remaining contaminants. 4 Dry the sample tray, autosampler cabinet, and autosampler arm using a dry towel. The autosampler must be thoroughly dry before you turn the power on. Weekly Cleaning Although cleaning it daily removes spills and contaminants from most of the autosampler components, it is necessary to clean the autosampler more thoroughly once a week. To do so, complete the following steps: 1 Shut down and unplug the autosampler. 2 Remove the sample tray. For information about removing the sample tray, see Replacing the Sample Tray later in this chapter. 3 Wipe the autosampler exterior and base until they are clean, using a towel dampened with a lab-grade cleaning agent, followed by a towel dampened with clear water. Pay special attention to the slider block which attaches the Z-drive assembly to the autosampler arm. 4 Wash the sample tray in a warm detergent solution. Make sure you remove all spills and stains. 46

47 Chapter 5: Maintaining the Autosampler 5 Rinse the sample tray with water and then dry it. Ensure that the sample tray is thoroughly dry. 6 Replace the sample tray on the autosampler base. For information about replacing the tray, see Replacing the Sample Tray later in this chapter. Cleaning the Lead Screws and Guide Rail NOTICE Never lubricate the lead screws or guide rail. The lead screw nuts are compounded with a dry film lubricant. Oiling the lead screws will cause gumming, galling, and binding of the moving parts of the autosampler. NOTICE Do not clean the lead screws or guide rail with any kind of solvent. If you can see loose particles on one of the lead screws or if particles are impairing movement: 1 Wipe away the loose particles with a dry, lint-free cloth. FIGURE 5-4 Y-Axis Lead Screw 47

48 Chapter 5: Maintaining the Autosampler Lead Screw Guide Rail FIGURE 5-5 X-Axis Guide Rail and Lead Screw (Viewed With Autosampler Front Cover Removed) Cleaning the Sample Probe Carbon Fiber (Coolants) Probes 1 Moisten a Kimwipes wiper with 10% HNO3 and wipe the probe to remove any aqueous or inorganic contamination from the surface. 2 Then moisten a new Kimwipes wiper with a chromatography grade solvent such as n-propanol, isopropanol, or hexane and wipe the probe again to remove any oil. 3 Let the probe air dry. Be careful not to touch the probe without gloves residue from handling the probe can cause the surface tension properties of the probe surface to change. Periodic cleaning may be necessary depending on the application. Stainless Steel (Oils) Probes 1 Wipe the probe with a lint-free laboratory wiper such as a Kimwipes wiper. Removing Tubing from Fittings NOTICE Do not pull hard on tubing to remove it from barbed fittings. Fittings can be damaged if too much force is applied. Instead, carefully cut the tubing. 1 First, try pulling the tubing off of the fitting. Apply a steady, linear force when removing the tubing to prevent the fittings from breaking. 48

49 Chapter 5: Maintaining the Autosampler Tubing can harden over time, which can make it extremely difficult to remove without damage. If the tubing does not come off of the fitting, you will have to cut it off: 2 Use a sharp blade to cut off the tubing just beyond the end of the fitting. FIGURE 5-6 Cutting Off the End of the Tubing 3 Carefully cut along the length of the tubing and peel it away from the fitting. FIGURE 5-7 Removing Tubing from the Fitting Replacing Peristaltic Pump Tubing Routine maintenance of the autosampler includes replacement of the peristaltic pump tubing. This tubing will probably be the most frequently replaced item on the autosampler. If you use strong bases, acids, or solvents as rinse agents, the tubing may break down rapidly. The tubing is installed as a single cartridge. The plates at the ends of the cartridge are color coded according to the tubing size and material. FIGURE 5-8 Peristaltic Pump Tubing Cartridge 49

50 Chapter 5: Maintaining the Autosampler ID (mm) Endplate Color 2, 2 Black 2, 3 Red 3, 3 White TABLE 5: Sample Probe Sizes and Color Coding To replace the peristaltic pump tubing, complete the following steps: WARNING PINCH HAZARD Turn off power before servicing the peristaltic pump or pump tubing. 1 Shut down and unplug the autosampler. 2 Release the pressure shoe by lifting the handle. The shoe is a flexible band which is held in place by spring-loaded handles. FIGURE 5-9 Releasing the Peristaltic Pump Shoe 3 Lift the end of the tubing cartridge straight up. FIGURE 5-10 Removing the Peristaltic Pump Tubing Cartridge 50

51 Chapter 5: Maintaining the Autosampler 4 Replace the tubing by sliding the plates on the end of the tubing cartridge into the slots in the pump. 5 Reinstall the pressure shoe. Replacing the Rinse Station Tubing If the rinse station tubing is typically exposed to deionized water as a rinsing agent, you do not need to replace it often. However, if you use other rinsing agents, such as acids or solvents, the tubing is likely to deteriorate more rapidly. To replace the rinse station tubing, complete the following steps: 1 Shut down and unplug the autosampler. 2 Move the autosampler arm 20 to 30 centimeters away from the home position by gently pushing it. Moving the autosampler arm ensures that the sample probe will not be damaged while you replace the rinse station tubing. 3 Disconnect the tubing between the rinse station and the peristaltic pump(s). Apply only a linear force when removing the tubing to prevent the fittings from breaking. 4 Connect the new rinse solution uptake and drain tubing. Apply only a linear force when replacing the tubing to prevent the fittings from breaking. 5 Move the autosampler arm back to the home position. Removing the Front Cover You will need a Phillips screwdriver. 1 Turn off the power switch on the back of the autosampler. 2 Unplug the autosampler from the power supply. 3 Disconnect all of the various serial and other cables that normally attach to its back. 4 Remove the screws that hold the front cover. There are two screws on the front, and either two (Oils 7400) or four (Oils 7600) screws on the top of the autosampler. 51

52 Chapter 5: Maintaining the Autosampler FIGURE 5-11 Location of Screws for the Top Cover 5 Lift the white block on the top of the autosampler just a little while you carefully pull the cover forward. Opening the Chain The cables for the stirrer motor and Z-drive motor pass through a plastic chain. If necessary, you can open the chain by pulling with your fingers or carefully prying with a small screwdriver. FIGURE 5-12 Opening the Plastic Chain 52

53 Chapter 5: Maintaining the Autosampler Replacing the Battery The battery in the autosampler is used to store diagnostic data in SRAM memory. The battery does not need to be replaced for routine operation of the autosampler. CAUTION FIRE AND EXPLOSION HAZARD Replace only with a CR2032 (3.0V, non-rechargeable lithium coin cell) or equivalent battery. Using an incorrect battery may cause fire, explosion, or leakage, resulting in personal injury or equipment damage. Do not open battery, dispose in fire, heat above 100C (212F), expose contents to water, recharge, or put in backwards battery may explode or leak and cause personal injury. 1 Turn off the power switch on the back of the autosampler. 2 Unplug the autosampler from the power supply. 3 Disconnect all of the various serial and other cables that normally attach to its back. 4 Remove the front cover (see page 51). 5 Note the orientation of the battery, then pull it out. Battery FIGURE 5-13 Location of the Battery 6 Insert the new battery. 7 Replace the cover. 8 Reconnect the power cord and turn on the power switch. 9 Recycle or dispose of the battery in accordance with local regulations. Aligning the Autosampler An autosampler may need alignment if: A motor or leadscrew has been replaced. 53

54 Chapter 5: Maintaining the Autosampler The autosampler has been subjected to extreme shock or vibration. The probe is not centered over the rinse station or over any of the sample vials. (See Alignment Problems on page 56 for some things to try before aligning the autosampler.) An alignment utility is provided on the CD. For instructions, see the Alignment Guide on the CD or the online help in the alignment utility. Note that there are several kinds of alignment, some of which can be performed with the software alone, and some which require the use of special tools or fixtures. Before running the alignment utility, you should turn off Smart Stir in the Autosampler Config utility. Checking the Home Position Alignment 1 Make sure the autosampler is turned off. 2 Turn the power on. This will move the sample probe to the home position. 3 Turn the power back off. 4 Visually check that the sample probe is centered in the rinse station. 5 If the sample probe is not centered, use the alignment utility to adjust the home position. Preparing for Long-Term Storage If you will not be using the system for a long time (a few weeks or more), you can increase the life of the components by making sure everything stays dry. If the System Has Never Been Used If the system has never been used, store it in a cool, dry place. If the System Has Been Used 1 Flush the lines to remove water and contaminants. Spectroscopy grade isopropyl alcohol works well for this purpose. 2 Store in a cool, dry place. 54

55 6 Troubleshooting In the event that the product does not function properly, isolate the problem to determine if it originates in the host computer, the analytical instrument, or the autosampler. This chapter explains how to troubleshoot autosampler problems. If you cannot solve a problem using the steps given in this chapter, you should contact Teledyne CETAC Technologies Customer Service and Support. Status LED The LED on the front of the autosampler has the following modes: Off: The autosampler is powered off. Steady Blue: The autosampler is powered on, the rinse station is in the coolants position, and the coolants peristaltic pump is selected. Steady Green: The autosampler is powered on, the rinse station is in the oils position, and the oils peristaltic pump is selected. Red: The autosampler is powered on but is uninitialized or an error has occurred. Flashing Blue and Green: The rinse station is not installed or there is a sensor error. 55

56 Chapter 6: Troubleshooting Alignment Problems If the probe is not centered over the rinse station or over any of the sample vials: 1 Check that the tray is seated on the autosampler base. The pins on the tray should be inserted in the holes in the base, the bottom of the tray should be flush against the base, and the tray should not move if you try to wiggle it. FIGURE 6-1 Autosampler Tray Not Seated (Left) and Seated (Right) 2 If the misalignment is only at the rinse station, check that the rinse station thumbscrew is snug. 3 If the misalignment is only for samples, check the ASX-7x00 Dashboard software (see page 39) and your instrument control software to make sure the correct rack type is selected. 4 Check that the probe/stirrer block and rinse station have the right spacing for the racks you are using. 5 Align the autosampler as described on page 53. Power System Problems A possible cause of system malfunction is a problem in the power system. If the system is not functional, it is possible that it is not receiving power. If this is the case, the LED power indicator light on the front of the autosampler will be off. To troubleshoot this problem, complete the following steps in sequence until the problem is solved: 1 Check that the power switch on the autosampler is turned on. 2 Check that the power cord is plugged in firmly between the power input on the autosampler and the external power supply and also between the power supply and the wall outlet. If the cable is plugged in, ensure that it is not damaged in any way. 3 Check that the LED on the power supply is illuminated. If the LED is not illuminated, check the wall outlet using a device approved for that purpose. 56

57 Chapter 6: Troubleshooting If the wall outlet is working and the voltage is acceptable, unplug the external power supply from the autosampler, but leave it plugged into the wall. If the LED is not lit, the power supply is faulty and requires replacement. If the LED is lit when unplugged from the autosampler but turns off when plugged into the autosampler, the autosampler may have an internal short and require repair. 4 If the external power supply LED is illuminated while plugged into the autosampler, cycle the power switch. The status LED on the front of the autosampler should light up. If the cords are properly connected, power is available, the external power supply is good, and the unit still does not initiate, continue by checking for communication problems and software setup problems. Communications Interface Problems Operation of the autosampler is directed by the host computer. A malfunction can indicate a problem with the RS-232 or USB cable (connected between the host computer and the autosampler) or with the configuration of the software on the host computer. The following sections explain how to troubleshoot these problems. RS-232 Serial Cable Problems 1 Check the host computer to ensure that the RS-232 cable is connected to the appropriate COM port. If the cable is plugged in, ensure that it is tightened properly and not damaged in any way. 2 Check that the cable is plugged into the COM1 (not COM2) port on the autosampler. 3 Check the serial port settings (9600, N, 8, 1). If the wrong port or baud rate is selected, change the configuration. USB Cable Problems Either RS-232 or USB may be used, but not both at once. 1 Check the USB cable to ensure that it is plugged into the port on the back of the autosampler. 2 Check the host computer to ensure that the USB cable is connected to the appropriate USB port. 57

58 Chapter 6: Troubleshooting NOTE If you move the USB cable to a different USB port, the driver may assign a new COM port number. 3 Check that the USB cable is not damaged in any way. Software Configuration Problems If the communication cable is connected properly and the system is still not functioning with the instrument control software, ensure that the software is communicating correctly with the autosampler. To do so, use the manual controls in the software to turn the peristaltic pump on and off. If the instrument control software fails to operate the autosampler, check that the same COM port number is configured in the software and in the USB device driver. Contamination and Carryover Problems Contamination and carryover can come from many sources. There are three basic questions to ask yourself: Is my rinse solution clean? Are surfaces being adequately rinsed? Are there hidden reservoirs (such as rough spots on wetted surfaces) where contaminants could be hiding? Here are some specific things to check: Rinse Solution / Reagent Problems 1 Ensure that the rinse reservoir level is high enough for the end of the intake tube to be submerged. 2 Ensure that the waste line completely empties prior to dilution. If it is not emptying completely the waste container may be full or the tubing may have been damaged or is restricted. Check the end of the rinse drain tube it should be above the surface of the liquid in the waste container. Empty the waste container. Remove restrictions in tubing and replace if necessary. 3 Replace the rinse solution if necessary, especially if the rinse solution is being recycled. 4 If using an internal standard, test the solution and replace it if necessary. System Configuration Problems 1 Check the autosampler rinse station tubing configuration. Rinse solution should flow upward from the bottom of the rinse station. 58

59 Chapter 6: Troubleshooting 2 Examine the sample transfer tubing which goes to the ICP. Make sure that it is as short as possible while still free to move. Make sure that there are no unnecessary fittings on the sample transfer tubing fittings can create small reservoirs where contaminants can hide. 3 Check that fittings and ferrules are properly tightened. Loose connections can result in syphoning. Worn / Contaminated Component Problems 1 If the tubing is more than six months old, or if it shows signs of contamination, replace it. 2 Replace the autosampler peristaltic pump tubing. 3 Replace the ICP peristaltic pump tubing. 4 Watch for liquid spilling from the drip cup as it moves. Empty the drip cup if necessary. It may be necessary to slow the autosampler down to reduce spilling. This can be adjusted using the ASX-7x00 Dashboard utility. Returning the Product to CETAC for Service Refer to the following information if you need to return the product to Teledyne CETAC Technologies for service. Shipping the Product Follow these guidelines when shipping the product: Use the original packing materials. If the original shipping materials are not available, you may be able to purchase packaging from Teledyne CETAC. Alternatively, place a generous amount of shock-absorbing material around the instrument and place it in a box that does not allow movement during shipping. Seal the box securely. Contact Teledyne CETAC Technologies before shipping the product. See Where to Go for More Information on page 14 for contact information. Have the model, serial number, and a description of the issue ready. You will be given an RMA (return merchandise authorization) number to track the instrument. Write the following information on a tag and attach it to the product: Name and address of the owner Product model number and serial number RMA number Description of service required or failure indications Pre-pay all shipping expenses including adequate insurance. Clearly label the box with the RMA number. If the RMA number is not visible on the outside of the box, Teledyne CETAC reserves the right to decline delivery and return the unopened box to you at your expense. 59

60 Chapter 6: Troubleshooting Mark the shipping container as FRAGILE. In all correspondence, refer to the instrument by model name or number and full serial number. Do not return products which are contaminated by radioactive materials, infectious agents, or other materials constituting health hazards to CETAC employees. Product Warranty Statement NOTE Contact Teledyne CETAC Technologies or refer to the warranty card which came with your product for the exact terms of your warranty. 60

61 7 Safety and Regulatory Information Review this product and related documentation to familiarize with safety markings and instructions before you operate the instrument. Characteristics Environmental Characteristics These environmental characteristics indicate the conditions for safe operation. The recommended environment for the best performance may be different. Operating Temperature +5 C to +40 C (+41 F to +104 F) Non-Operating Temperature +0 C to +55 C (+32 to +131 F) Operating Altitude Operating Relative Humidity Non-Operating Relative Humidity Up to 2,000 m (6,562 ft) 0% to 80% non-condensing for temperatures up to 31 C, decreasing linearly to 50% at 40 C 0% to 95% non-condensing Pollution Degree Pollution Degree 2 Normally no pollution or only dry, nonconductive pollution occurs. The pollution has no influence. Occasionally, however, a temporary conductivity caused by condensation may be expected. TABLE 6: Environmental Characteristics For indoor use only. Avoid sudden, extreme temperature changes which could cause condensation on circuit boards in the product. 61

62 Chapter 7: Safety and Regulatory Information See page 12 for information on chemical compatibility. Electrical Characteristics Power requirements Power Supply (Provided with Autosampler) Autosampler Input: AC Voltage, Frequency, and Current V ~ Hz 1.07 A Installation Category: CAT II (Line voltage in appliance and to wall outlet) Output: 24 V DC, 3.33 A Input: DC Voltage and Current 24 V 3.33 A Installation Category: CAT I (Mains isolated) Use only with the provided power supply. TABLE 7: Power Requirements Input and output connectors COM1 COM2 USB ETHERNET Z-DRIVE STIRRER DB-9 RS-232 serial connection to a controller PC. (max ±12 VDC, 8 ma) DB-9 RS-232 serial connection to accessories or instruments. (max ±12 VDC, 8 ma) USB connection to a controller PC. (max 5 VDC) Ethernet connection. (Disabled by default.) 15-pin power and signal connection for Z-drive. 24V max output. Connect only to the supplied Z-drive, using the cable which is part of the Z-drive assembly. Power and signal connection for stirrer. 24V max output. Connect only to the supplied Z-drive assembly. 62

63 Chapter 7: Safety and Regulatory Information AUXILIARY Additional connectors may be provided for specific applications. The standard auxiliary connector has the following connections: Unavailable, DO NOT USE Inputs: Optically isolated inputs to insure instrument protection from ancillary devices. Can be interfaced to a relay (dry contact) Can be interfaced to an open collector transistor (wet contact) Outputs: Normally open isolated solid-state relay N/O = Normally Open Max voltage = 24 V (DC only) Max current = 350 ma (750 ma on some models manufactured before 2016) Solenoid Output: Outputs 24 V DC for 0.5 sec, then applies 150 Ohm current-limiting resistor with FC_SOL command. When connected to a solenoid found in some CETAC accessories, the output voltage is 24 V DC for 0.5 sec, then drops to about 15 V DC. Exact performance may vary, depending on the solenoid model connected. TABLE 8: Electrical Input and Output Connectors on the Autosampler 63

64 Chapter 7: Safety and Regulatory Information Safety Notices WARNING INJURY HAZARD If the equipment is used in a manner not specified by Teledyne CETAC Technologies, the protection provided the equipment may be impaired. Repair or service that this not covered in this manual should only be performed by qualified personnel. Power Cord Set Requirements The power cord set supplied with your instrument meets the requirements of the country where you purchased the instrument. Power Cord Safety Maintenance The operator should check the power/signal supply cord condition. The equipment should not be operated if the mains inlet is cracked or broken. Any obvious damage to the case (from a drop or fall) should be checked by service personnel for loose or damaged parts. See individual parts lists for approved replacement parts. Mains Disconnect The power switch on the rear panel is not the mains disconnect. Power mains disconnect is accomplished by unplugging the power cord at the power supply or at the wall outlet. Ensure the power cord is easily accessible and removable, in the event of an emergency which requires immediate disconnection. WARNING SHOCK HAZARD Ensure that power cord is disconnected before removal of any covers. 64

65 Chapter 7: Safety and Regulatory Information Cleaning Instructions For additional cleaning information, see cleaning in the index. To clean the exterior surfaces of the instrument, complete the following steps: 1 Shut down and unplug the instrument. 2 Wipe the instrument exterior surfaces only using a towel dampened with a lab-grade cleaning agent. 3 Repeat step 2, using a towel dampened with clear water. 4 Dry the instrument exterior using a dry towel. WARNING SHOCK HAZARD Do not allow any liquid to enter the instrument cabinet other than as intended through the specified tubing, or come into contact with any electrical components. The instrument must be thoroughly dry before you reconnect power, or turn the instrument on. Mechanical Hazards If you insert any part of your body between the moving parts of the equipment, you could be injured. FIGURE 7-1 shows the location of potential hazards FIGURE 7-1 Overview of the Mechanical Hazards of the Autosampler CAUTION 1 - PINCH HAZARD Keep fingers, hair, and loose clothing away from the moving parts of the autosampler. 2 - PUNCTURE/INJECTION HAZARD Keep hands away from the probe while the autosampler is operating. 65

66 Chapter 7: Safety and Regulatory Information Operating Environment WARNING SHOCK HAZARD To reduce the risk of fire hazard and electrical shock, do not expose the unit to rain or humidity. To reduce the risk of electrical shock, do not open the cabinet. All maintenance is to be performed by an Authorized CETAC Service Provider. Protection provided by the equipment may be impaired if the equipment is used in a manner not specified by the manufacturer. WARNING SHOCK HAZARD Equipment is not intended for wet locations. Miscellaneous liquids in the equipment could cause hazardous conditions. WARNING EXPLOSION HAZARD Do not operate in an explosive atmosphere. WARNING CHEMICAL HAZARDS Learn about the chemicals which will be used in and near the autosampler, and observe the necessary precautions. Always use appropriate personal protective equipment, including protective eyewear, lab coat, and gloves. Explanation of Caution and Warning Notices Warning symbol marked on equipment. This symbol means Attention! Refer to the manual. Crush Hazard / Pinch Point Keep hands clear of moving parts. X, Y, Z axis movement may crush hand. Lifting Hazard Single person lift could cause injury. Use assistance when moving or lifting. 66

67 Chapter 7: Safety and Regulatory Information WARNING A WARNING indicates a hazardous situation, which, if not avoided, could result in death or serious injury. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood. CAUTION A CAUTION indicates a hazardous situation, which if not avoided, could result in minor or moderate injury. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met. NOTICE A NOTICE calls attention to a procedure or practice that if not correctly performed or adhered to, could result in equipment damage, loss of data, or inaccurate data. A NOTE emphasizes a point, provides a useful tip, or provides additional information. Electromagnetic Interference FEDERAL COMMUNICATIONS COMMISSION (FCC) NOTICE This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a commercial installation. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. Operation of this equipment in a residential environment is likely to cause harmful interference, in which case the user will be required to correct the interference at his expense. MODIFICATIONS The FCC requires the user to be notified that any changes or modifications made to this device that are not expressly approved by Teledyne CETAC Technologies may void the user's authority to operate the equipment. CABLES Connections to this device must be made with shielded cables with metallic RFI/EMI connector hoods to maintain compliance with FCC Rules and Regulations. 67

68 Chapter 7: Safety and Regulatory Information CANADIAN NOTICE This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the interference-causing equipment standard entitled "Digital Apparatus" ICES-001 of the Department of Communications. AVIS CANADIEN Cet appareil numerique respecte les limites de bruits radioelectriques applicables aux appareils numeriques de Classe A prescrites dans la norme sur le materiel brouilleur: "Appareils Numeriques," NMB-001 edictee par le ministre des Communications. KOREAN NOTICE 사용자안내문 이기기는업무용환경에서사용할목적으로적합성평가를받은기기로서가정용환경에서사용하는경우전파간섭의우려가있습니다. Warning Statement EMC Registration is done on this equipment for business use only. The product may cause interference when used in a home. This warning statement applies to a product for business use. Explanation of Regulatory Marks Do not dispose in domestic household waste. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste, in compliance with the European Waste Electrical and Electronic Equipment Directive (WEEE, 2002/96/EC). For instructions on how to return end-of-life equipment, producer-supplied electrical accessories, or auxiliary items for proper disposal please contact the supplier or importer. In the event a supplier cannot be reached, contact Teledyne CETAC Technologies customer service department at 1 (800) The CE mark is a registered trademark of the European Community. This CE mark shows that the product complies with all the relevant European Legal Directives. 68