MEA - Microelectrode Systems
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1 - Microelectrode Systems
2 Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH. While every precaution has been taken in the preparation of this document, the publisher and the author assume no responsibility for errors or omissions, or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it. In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document Multi Channel Systems GmbH. All rights reserved. Printed: Multi Channel Systems MCS GmbH Aspenhaustraße Reutlingen Germany Fon Fax Products that are referred to in this document may be either trademarks and/or registered trademarks of their respective holders and should be noted as such. The publisher and the author make no claim to these trademarks.
3 About Systems MCS provides complete solutions for stimulation, recording, data acquisition and analysis. The product line is perfect for in vitro applications, that is, acute slices, cultured tissues, and cells on microelectrode arrays (s). This guide gives you an overview over the various products and possible combinations. The illustrations show how the devices are set up and connected. These setups are to be understood as suggestions; you can define your custom setup as well. Please refer to the separate documentation to obtain more detailed information about single components, installation, or operating issues. Do not hesitate to contact MCS or your local retailer if you are interested in a particular setup or if you have other questions. Data Acquisition and Analysis 60System is a complete system for in vitro recording from microelectrode arrays (s), perfusion, and data acquisition from up to 64 channels. Raw data from up to 60 electrodes is amplified by a 1060 amplifier, and then acquired by the of the connected computer. Recorded data is graphed, analyzed, reviewed, and exported with powerful and easy-to-use. The temperature controller TC0X regulates the temperature. 60SystemE is an alternative version of the system with an advanced perfusion, featuring a perfusion cannula PH01 with programmable fluid temperature and a two-channel temperature controller TC02. The computer supplies power via the for all devices of the setup, except the TC0X and the STG100X. MCS standard cables (3 meters), C68x1M (1 meter), or C68x0.5M (0.5 meter) are used for connecting the channels. 120System has a extension that allows recording from two s with 120 electrodes in total. bined with a S4to2, you can even record from four s simultaneously. Choose any of the up to 120 recording channels conveniently by mouse-click with the _Switch software. Or use a S2to1 to select up to 60 channels from two s with a standard 60System or 60SystemE. All systems are available either for inverted microscopes or for upright microscopes, 60(E)- 1System and 60(E)-2System, respectively. The breakout box BB permits to select any channel and view it with an external oscilloscope. Stimulation The flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). Waveforms designed in the program or imported from an external file are converted by a stimulus generator STG100X into pulses, which are send to stimulating electrodes. No external stimulus isolations units are required. Stimulation and recording can be synchronized with a digital trigger signal (TTL) sent from the SyncOut output of the STG100X to the via the digital in/out box (D i/o). If you prefer the new 1060BC amplifier, software controls allow you to select any electrode on a for stimulation and recording. A 500 µs long blanking signal switches off stimulating electrodes during stimulation and thus completely removes stimulus artifacts, making it possible to use stimulating electrodes for recording as well.
4 Setups in this Guide Overview STG100X * TC0X* 1060* 1060BC BB S4to2 60System 60SystemE 120System Stimulation Microelectrode array Temperature controller Amplifier Amplifier No stimulus artifacts Breakout box switch Data acquisition / computer Data acquisition / computer Data acquisition / computer Setup 1 x x x x Setup 2 x x x x x Setup 3 x x x x x Setup 4 x x x x x Setup 5 x x x x x x Setup 6 x x x x Setup 7 x x x x x Setup 8 x x x x x Setup 9 x x x x x Setup 10 x x x x x x Setup 11 4 x x 4 x x x * = Part of corresponding System
5 60System 64 Channel data acquisition system with 1060 amplifier, temperature controller TC01, and -PPORT2 perfusion port puter TC PPORT2 The power for all devices is supplied by the of the connected computer. puter power supply
6 60System with BB Single channels of interest can be picked with BB and viewed with an external oscilloscope. puter TC C68x1M BB -PPORT2 The power for all devices except the TC0X is supplied by the of the connected computer. puter power supply
7 60System with STG For stimulating and recording. Flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). No external stimulus isolations units are required. puter TC01 MC_Stimulus PPORT2 Stimulus STG100X STG1002 STG1001 SyncOut trigger Stimulus generator control via RS232 The power for all devices except the STG100X is supplied by the of the connected computer. D i/o puter power supply
8 60System with STG and Blanking Circuit For stimulating and recording. Flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). No external stimulus isolations units are required. Software controls allow you to select any electrode on a for stimulation and recording. A blanking signal continuing for 500 µs after a stimulus completely removes stimulus artifacts, making it possible to use stimulating electrodes for recording as well. puter Electrode selection via RS232 TC01 MC_Stimulus _Select 1060BC -PPORT2 Stimulus Blanking signal STG1002 STG1001 STG100X SyncOut trigger Stimulus generator control via RS232 The power for all devices except the TC0X and the STG100X is supplied by the of the connected computer. D i/o puter power supply
9 60System with STG with BB For stimulating and recording. Flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). No external stimulus isolations units are required. Single channels of interest can be picked with BB and viewed with an external oscilloscope. puter TC01 MC_Stimulus 1060 C68x1M BB -PPORT2 Stimulus STG100X STG1002 STG1001 SyncOut trigger Stimulus generator control via RS232 The power for all devices except the STG100X is supplied by the of the connected computer. D i/o puter power supply
10 60SystemE 64 Channel data acquisition system with 1060 amplifier, temperature controller TC02, and PH01 perfusion cannula puter TC02 PH PPORT2 The power for all devices is supplied by the of the connected computer. puter power supply
11 60SystemE with BB Single channels of interest can be picked with BB and viewed with an external oscilloscope. puter TC02 PH C68x1M BB -PPORT2 The power for all devices except the TC02 is supplied by the of the connected computer. puter power supply
12 60SystemE with STG For stimulating and recording. Flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). No external stimulus isolations units are required. puter TC02 PH01 MC_Stimulus PPORT2 Stimulus STG100X STG1002 STG1001 SyncOut trigger Stimulus generator control via RS232 The power for all devices except the STG100X is supplied by the of the connected computer. D i/o puter power supply
13 60SystemE with STG and Blanking Circuit For stimulating and recording. Flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). No external stimulus isolations units are required. Software controls allow you to select any electrode on a for stimulation and recording. A blanking signal continuing for 500 µs after a stimulus completely removes stimulus artifacts, making it possible to use stimulating electrodes for recording as well. puter Electrode selection via RS232 TC02 PH01 MC_Stimulus _Select 1060BC Stimulus Blanking signal D i/o SyncOut trigger STG1002 STG1001 STG100X Stimulus generator control via RS232 The power for all devices except the TC0X and the STG100X is supplied by the of the connected computer. puter power supply
14 60SystemE with STG with BB For stimulating and recording. Flexible MC_Stimulus software enables complex stimulus waveforms (both current and voltage). No external stimulus isolations units are required. Single channels of interest can be picked with BB and viewed with an external oscilloscope. puter TC02 PH01 MC_Stimulus 1060 C68x1M BB -PPORT2 Stimulus STG100X STG1002 STG1001 SyncOut trigger Stimulus generator control via RS232 The power for all devices except the STG100X is supplied by the of the connected computer. D i/o puter power supply
15 120System with four 1060 amplifiers and S4to2 Record from up to 120 channels from amplifiers, i. e. from 4 s, simultaneously. Choose any recording channel conveniently by mouse-click in the _Switch software. puter _Switch 1060 SB8x C68x1M 240 Ch S4to2 120 Ch S4to2 control via RS232 puter power supply The power for all devices is supplied by the of the connected computer. Important: You need an extension card to acquire data from up to 128 channels with. The standard has 64 input channels.
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