Customers should provide all necessary operating supplies upon request of the engineer.

Transcription

1 Agilent Installation Infinity Checklist II Preparative HPLC System Thank you for purchasing an Agilent instrument solution. This checklist is used by the installing engineer to ensure that the instrument and associated systems are correctly installed, upgraded and functioning as designed in your facility. This checklist will be completed at the end of the service and provided to you as a record of the installation. Customer Information Customers should provide all necessary operating supplies upon request of the engineer. Service Engineer s Responsibilities Only complete/printout pages that relate to the system being installed. Complete empty fields with the relevant information. Complete the relevant checkboxes in the checklist using a X or tick mark in the checkbox. Complete Not Applicable check boxes to indicate services not delivered, as needed. Complete the Service Review section together with the customer. Additional Instruction Notes A checkout test is available. The delay calibrant ( ) can also be used as checkout solution when injected onto a column and run with a gradient. The result should show three colored compounds which are collected in three different fractions. At the end of the installation, run the System Test (described below). System Test 1 Ensure that a ZORBAX SBC18 PrepHT 21.2 mm (or 9.4 mm) 50 mm, 5 µm column is connected, and that the LC system is properly primed. 2 Check that the mobile phase and needle wash solvents previously used by the customer are compatible with the solvents used for the System Test. If not, use an appropriate intermediate solvent to prepare the system.

2 3 Create a method Checkout.m with the following details: Sample: Delay Calibrant Eluent A: Eluent B: Flow: 0.1 % TFA in LC grade water 0.1 % TFA in ACN 25 ml/min (5 ml/min on 9.4 mm column) Gradient: 0.0 min 98% A 2% B Runtime: Injection volume: Peak Detector: Peak Det. Mode: 0.3 min 98% A 2% B 3.5 min 2% A 98% B 4.2 min 2% A 98% B 4.3 min 98% A 2% B 5.5 min 98% A 2% B 5.5 min Optional (if Mass Based system): Modulator Mode: 50 µl (10 µl on 9.4 mm column) UV or DAD signal A, UV 310 nm (+/ 4 nm, 5 Hz, no reference) Threshold (5 mau) and Slope (50 mau) M1 1000:1 (M6 1000:1 with 9.4 mm column) Molecular weights: Peak 1, Sunset Yellow (M=452.37) MNa 429 neg, Makeup flow: Peak 2, Patent Blue (M=566.66) MS Det. Mode: Threshold Peak 3, Sudan Orange (M=214.22) MNa 203 neg (double charge) MNa 543 neg, MNa+H 545 pos, M+H 567 pos M+H 215 pos 1.5 ml/min with only 33 % of this entering the MSD (Teeline split) 4 Ensure that 3 peaks are observed and three fractions are collected (yellow, blue and orange) and enter the results in the results table at the end of this checklist.

3 System Information Guidance: Check box if instrument configuration report is attached instead of completing the table below. Instrument System Name/I.D: Record the list of system component product numbers below. Instrument Location: List the serial numbers of the components present in the system below

4 General Preparation Unpack/verify the condition and completeness of shipment. For discrepancies, use the table below. Product/Part Description Observation Action Discuss any specific questions or issues with the customer prior to starting. Discuss any configuration options with the customer prior to starting Check for required service note applicability and firmware updates if connecting to instruments. Check that customersupplied PC hardware meets minimum requirements e.g. Site Preparation Tool Upgrades only Ensure with customer that instrument control settings, data, methods etc have been properly saved or archived prior to starting any installation procedures. Measure the LC/MSD outlet line voltage: VAC

5 G9328A 1260 Infinity II Preparative Column Organizer Leak Plane on the bench Place Mounting Plate on Leak Plane Attach Leak Sensor Mount Clamps on to Stack Module Attach the Manual Injector (if installed) Manual injector inlet/outlet tubing Sample loop Contact closure wiring Attach the Column Holders (if installed) Add Column to Column Holder Column inlet/outlet tubing Install the column Attach Pressure Restrictor holder Attach Leak Tube

6 G7163B 1290 Infinity II Preparative Column Compartment Attach Leak Sensor Waste Drain Optional: Mount all available valve based modules Attach the Manual Injector (if installed) Manual injector inlet/outlet tubing Sample loop Contact closure wiring Attach the Column Holders (if installed) Add Column(s) to Column Holder(s) Column inlet/outlet tubing Attach Extraction Hose (if used) Attach Pressure Restrictor holder Attach Leak Tube

7 G7161A 1260 Infinity II Preparative Binary Pump Set up the Pump Solvent Inlet/Outlet Waste Drain Remote Control (ERI) (if used) Seal Wash tubing CAN cable Power cable Configure Communications Optional: Install the Solvent Cabinet Optional: Install the External Leak Sensor Prime the Pump Prepare Solvents Turn on Purge Monitor the pressure, ripple should be <2 % Run a Leak Rate test

8 G1328D Preparative Manual Injector Set up the Preparative Manual Injector Mount injector to bracket holder Mount to Preparative Column Organizer Solvent Inlet Solvent Outlet Injection loop Waste Drain/Beaker Contact closure cable Prepare the sampler and fill the available channel with appropriate solvent from the solvent bottles. Prime inlet and outlet lines, with injection valve opened and closed

9 G7157A 1260 Infinity II Preparative Auto Sampler Set up the Sampler Solvent Inlet Waste Drain. (must be in a straight line down, no bends, no kinks) check manual for further info Remote Control (ERI) (if used) CAN cable Power cable Configure Communications Prepare the sampler and fill the available channel with appropriate wash solvent from the solvent bottles. Turn on the module Configure Prime Activate Prime

10 G7114A/B 1260/1290 Infinity II Variable Wavelength Detector Set up the Detector Deuterium lamp (if not installed) Remote Control (if used) Analog Output (if used) Connect CAN cable Connect LAN cable (the detector should be always the host of the LAN communication with the PC) Power Cable Turn on the Detector and Lamp as soon as possible (minimum one hour warmup time) Flow Cell Solvent Inlet/Outlet Waste Drain Configure Communications Verify the operation of the Detector Perform the Lamp Intensity Test Perform the Wavelength Calibration Test Lamp Intensity WL calibration Pass/Fail Wavelength Deviation of 0Order Wavelength Deviation of Alpha Line (656 nm)

11 G7115/G7165A 1260 Infinity II Multi Wavelength/Diode Array Detector Set up the Detector Deuterium lamp (if not installed) Remote Control (if used) Analog Output (if used) Connect CAN cable Connect LAN cable (the detector should be always the host of the LAN communication with the PC) Power Cable Turn on the Detector and Lamp as soon as possible (minimum one hour warmup time) Flow Cell Solvent Inlet/Outlet Waste Drain Configure Communications Verify the operation of the Detector Perform the Lamp Intensity Test Perform the Wavelength Calibration Test Lamp Intensity WL calibration Pass/Fail D2 Alpha Line Deviation (486 nm) D2 Beta Line Deviation (656 nm)

12 G6100 Series Single Quadrupole Mass Spectral Detector Using two people, place the MSD on a stable bench or table. Make sure adequate space is available to operate and maintain the instrument. Install the spray chamber drain bottle. Install the rough pump and fill with oil (if applicable). Install the Agilent TS800 Dry pump (if applicable). Install the TS800 Scroll pump ship kit (if applicable). Connect the MSD vacuum hose to the rough pump. Install the rough pump exhaust KF fitting adapter. Connect the other end of the exhaust tubing hose to the rough pump exhaust vent. Connect the spray chamber exhaust tube between the desolvation assembly and 1" drain bottle fitting. Connect a length of 3/4" exhaust tubing to the drain bottle vent fitting. Connect the other end of the exhaust tubing hose to a separate exhaust vent from the rough pump. Attach the waste line from the MSD to the drain bottle 1/4" fitting. Connect the LC leak drain system by moving the MSD right front cover. Install RMSN4 nitrogen gas filter(s) using 1/4" PTFE tubing. Install RMSN4 filter between nitrogen supply for the MSD. Secure gas filter(s) vertically to suitable support(s). Turn on the nitrogen supply, set the regulator between psi ( kpa), and check for leaks. Connect the rough pump power cord to the MSD. Connect the MSD power cord to the power outlet. Connect the LAN cable to the MSD LAN port. Install the Remote cable between one LC module and the remote port of the MSD. Install the APCI enablement kit (only applicable for APCI or Multimode source). Install the Multimode enablement kit (only applicable for Multimode source). Uninstall the spray shield and remove the red GC septum from the capillary entrance. Reinstall the spray shield. Install the ES source (if applicable). Attach the source to desolvation assembly. Install and align the standard Spray Shield (G ) on the MSD. Connect the 1/8 nebulizer gas line from the MSD to the nebulizer gas fitting. Connect LC tubing from Flow Modulator to nebulizer ZeroDeadVolume fitting. G6125B/C and G6135B/C: Add G ESI tuning calibrant.

13 Install the APCI source (if applicable). Attach the source to desolvation assembly. Install and align the standard Spray Shield (G ) on the MSD. Connect the 1/8 nebulizer gas line from the MSD to the nebulizer gas fitting. Connect LC tubing from Flow Modulator to nebulizer ZeroDeadVolume fitting. Connect the APPI heater cable to the MSD Vaporizer Heater connection. Connect the male APPI power and communication cable to the DB9 connector on the source. Connect the female APPI power and communication cable to Serial B port on the MSD SmartCard. Add G APCI/APPI tuning calibrant. Install the Multimode source (if applicable). Attach the source to desolvation assembly. Install and align the Multimode Spray Shield (G ) on the MSD. Connect the 1/8 nebulizer gas line from the MSD to the nebulizer gas fitting. Connect LC tubing from Flow Modulator to nebulizer ZeroDeadVolume fitting. Connect the APCI high voltage cable to the MSD at the Auxiliary Module Corona connection. Connect the Multimode high voltage cable to the MSD at the Auxiliary Module Multimode connection. Connect the Multimode heater cable to the MSD Vaporizer Heater connection. G6125B/C and G6135B/C: Add G APCI/APPI tuning calibrant. Install the Jet Stream source (AJS) (if applicable). Attach the source to desolvation assembly. Install and align the AJS Spray Shield (G ) on the MSD. Connect the 1/8 nebulizer gas line from the MSD to the nebulizer gas fitting. Connect LC tubing from Flow Modulator to nebulizer ZeroDeadVolume fitting. Connect the 1/8 AJS sheath gas line from the Agilent Jet Stream to the MSD sheath gas fitting. Connect the AJS nozzle voltage cable to the MSD at the Auxiliary Module Multimode connection. Connect the AJS Sheath Gas heater cable to the MSD Vaporizer Heater connection. G6135B/C: Add G ESIL tuning calibrant. Turn on the MSD and allow the system to pump down. Verify the operation of the MSD Perform a Dual Polarity Autotune on the G6125B or G6135B (if applicable). Perform a Dual Polarity Checktune on the G6125C or G6135C (if applicable). Perform a background scan check from m/z 100 to 1000 at 0.4 ml/min: G6125B/C: TIC baseline should be less than counts. G6135B/C: TIC baseline should be less than counts.

14 G6125B/C: Perform a ES positive ion SIM checkout and confirm RMS signaltonoise of 30:1 G6125B/C: Perform an APCI positive ion SIM checkout and confirm RMS signaltonoise of 15:1 G6135B/C: Perform a ES positive ion SIM checkout and confirm RMS signaltonoise of 100:1 G6135B/C: Perform a ES positive ion Scan checkout and confirm RMS signaltonoise of 100:1 G6135B/C: Perform an APCI positive ion SIM checkout and confirm RMS signaltonoise of 50:1 G6135B/C: Perform an APCI positive ion Scan checkout and confirm RMS signaltonoise of 50:1 G6135B/C: Perform an AJS positive ion SIM checkout and confirm RMS signaltonoise of 500:1 G6135B/C: Perform an AJS positive ion Scan checkout and confirm RMS signaltonoise of 500:1 Fill out instrument logbook. Flush the LC overnight with 0.5 ml/min of 70:30 ACN:H 2 O with 0.1 % Formic Acid using the following spray chamber parameters: ESI source: Nebulizer Pressure 40 psi, Drying gas flow 10 L/min, Drying gas temperature 350 C. APCI or APPI source: Nebulizer Pressure 40 psi, Drying gas flow 5 L/min, Drying gas temperature 350 C, Vaporizer temperature 450 C. Multimode Source: Nebulizer Pressure 40 psi, Drying gas flow 5 L/min, Drying gas temperature 350 C, Vaporizer temperature 200 C. AJS Source: Nebulizer Pressure 40 psi, Drying gas flow 5 L/min, Drying gas temperature 350 C, Sheath gas temperature 350 C, Sheath gas flow 10 L/min, Nozzle voltage 0 V. Make sure the Switch Stream option is set to MSD. Bake out the system for at least 2 hours; preferably, bake out overnight. After the overnight flushing, flush with 100 % isopropanol for 30 minutes at 1 ml/min. Flush with 50:50 Methanol:Water for 30 minutes at 1 ml/min. Install the 2.1 x 50 mm x 1.8 µm SB18 Rapid Resolution column. Flush with 100 % methanol for 1 hour at 1 ml/min. Prepare LC mobile phase for positive ion verification; 70:30 ACN:H 2 O with 0.1 % Formic Acid Connect 1 liter of checkout solvent to Channel A and flush for 1 hour at 0.5 ml/min. Record system serial numbers and fill out installation documents while flushing.

15 Preparing Performance Evaluation Samples Use 5 ng/µl positive ion (reserpine) standard G2423A (P/N G ) and dilute to appropriate concentration. G6125B/C: SIM: Prepare a 1:5000 dilution (1 pg/µl) G6135B/C: SIM: Prepare a 1:5000 dilution (1 pg/µl), Scan: Prepare a 1:500 dilution (10 pg/µl) Perform a standard instrument verification for the installed source. If more than one source is present, perform verification with each source (see source specific section(s) for details).

16 G7170A 1290 Infinity II Flow Modulator Assemble Leak Tray, Tray Holder and Sensor Attach Leak Tray Assembly to Valve and connect Sensor Attach Mounting Clamps to Flow Modulator Optional: Attach Valve Rail Attach Valve to Valve Rail Plumb up the Valve and route all lines Set up the Valve Power cable CAN cable Configure Communications

17 G7166A 1260 Infinity II Preparative Valvebased Fraction Collector Assemble Leak Tray, Tray Holder and Sensor. Attach Leak Tray Assembly to Valve and connect Sensor. Attach Mounting Clamps to Fraction Valve. Optional: Attach Valve Rail to moduled. Attach Valve to Valve Rail. Remove all blocking nuts, plumb each port and route all lines to a suitable location. Set up the Valve: Power cable CAN cable Configure Communications Configure Valve Linked Pump Configure Valve Peak Detector Configure Max. Fill Volumes Configure Air Flushing Times Perform a Delay Calibration. Delay Calibration Linked Pump Model/SN Peak Detector Model(s)/SN Delay

18 G1364E 1260 Infinity II Preparative Fraction Collector Section NOT Applicable Set up the fraction collector(s) Solvent Inlet/Outlet Waste Drain Optional: connect hose to ventilation system Optional: install and connect G9322A Clustering Valve (for multiple G1364E only) Remote Control (if used) Relay Contacts (if installed) CAN cable Power cable Configure Communications Configure Linked Pump Configure Peak Detector Configure Max. Fill Volumes Perform a Delay Calibration Delay Calibration Linked Pump Model Peak Detector Model(s) Delay

19 G7159B 1290 Infinity II HiP Preparative OpenBed Fraction Collector Place the fraction collector on the bench. Remove transport foam and lock screw. Install the Tubing Kit. Install the Top Fume Hood: Screw the metal holder onto the frame. Install the Top Fume Hood. Install the Drawer(s) and place the container(s) on the drawer(s). Plumb the fraction collector and route all lines. Optional: connect hose to ventilation system. Optional: install and connect G9322A Clustering Valve (for multiple G7159B only). Set up the fraction collector: Power cable CAN cable Configure communications Configure a linked pump Configure a peak detector Perform the persistent calibration. Perform a Delay Calibration. Delay Calibration Linked Pump Model Peak Detector Model(s) Delay

20 G9324A 1260 Infinity II Delay Coil Organizer Attach Mounting Clamps to Delay Coil Organizer Optional: Attach Valve Rail to module Attach Delay Coil Organizer to Valve Rail Plumb up the required Delay Coils to the inside of the Delay Coil Organizer Plumb the inlet and outlet to the front of the Delay Coil Organizer (note that the outlet needs a PRV as instructed in the Tubing Kit documentation)

21 M8550A Lab Advisor Advanced Install the Lab Advisor software on the controlling PC Open the Lab Advisor software. Navigate to the License tab under Configuration and enter the Lab Advisor Advanced license M8555A Lab Advisor Basic Verify with customers if they would like it installed If applicable install the Lab Advisor software on the controlling PC Checkout Test Checkout Test performed Results entered in System Test Results table

22 Service Review Attach available reports or printouts to this documentation. Record the time and date of installation or upgrade completion in the customer s records or logbook. Complete the Service Engineer Comments section below if there are additional comments. Enter the results of the System Test in the section below. Review the installation or upgrade with the customer. Explain Agilent warranty for instruments. Explain how to use manuals, guides, online help. Explain how to get selfhelp, FAQs from the web. Explain how to log an instrument service call, support services available. Advise customer of additional instrument training options. If the instrument firmware was updated, record the details of the change in the Service Engineer Comments box below or if necessary, in the customer s IQ records. System Test Results System Test Observation Pass/Fail or Not Applicable Checkout Three peaks observed UV/DAD MSD Yellow fraction collected UV/DAD MSD Blue fraction collected UV/DAD MSD Orange fraction collected UV/DAD MSD

23 Service Engineer Comments (optional) If there are any specific points you wish to note as part of performing the installation or other items of interest for the customer, please write in this box. Service Completion Service Request number Date service completed Agilent Signature Customer Signature Other Important Customer Web Links How to get information on your product: Literature Library Need to know more? Need technical support, FAQs? Need supplies?