Ion Chef Instrument Network Connection

Transcription

1 Ion Chef Instrument Network Connection USER GUIDE Publication Number MAN Revision C.0 For Research Use Only. Not for use in diagnostic procedures.

2 Manufacturer: Life Technologies Holdings Pte Ltd Block 33 Marsiling Industrial Estate Road 3 #07-06, Singapore The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. Revision history: Pub. No. MAN Revision Date Description C.0 October 2, 2018 This revision includes information to: B.0 December 18, 2015 Various revisions. A.0 January 27, 2015 New document. Connect an Ion Chef Instrument to an Ion GeneStudio S5 Sequencer Share Planned Runs using Ion Mesh Monitor consumables using Ion Mesh Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified Thermo Fisher Scientific Inc. All rights reserved.

3 Contents CHAPTER 1 Introduction... 5 Overview... 5 About the Torrent Server... 5 Networking guidelines and best practices... 6 Network setup workflow... 6 Collect the required network information... 6 CHAPTER 2 Connections between Torrent Server and the Ion Chef Instrument... 7 General requirements... 7 Direct connection... 7 DHCP IP... 8 Static IP... 8 Add the Torrent Server IP address... 9 Edit the Torrent Server firewall tables to allow the LAN port IP Create a firewall exception for the Torrent Server Connect directly to the Torrent Server Connect by network to the Torrent Server CHAPTER 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument General requirements Direct connection DHCP IP Static IP Add the sequencer IP address Edit the Torrent Server firewall tables to allow the LAN port IP Create a firewall exception for the Torrent Server Connect directly to the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer Connect by network to the Ion S5 or Ion GeneStudio S5 Sequencer Ion Chef Instrument Network Connection User Guide 3

4 Contents CHAPTER 4 Monitor runs, share Planned Runs, and manage consumables with Ion Mesh Set up Ion Mesh View sequencing runs on multiple sequencers with Ion Mesh Transfer a Planned Run to a Torrent Server with Ion Mesh Remove a server from an Ion Mesh configuration Change server nickname in an Ion Mesh configuration Enable or disable an Ion Mesh connection Track Ion Chef flexible workflows for Ion 550 chips with Ion Mesh Related documentation Customer and technical support Limited product warranty Ion Chef Instrument Network Connection User Guide

5 1 Introduction If your Torrent Server, Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus sequencer was not connected to the Ion Chef Instrument during installation, or if you move the equipment in your laboratory, the procedures in this guide might apply to you. Overview This document describes how to configure the Ion Chef Instrument to connect with a Torrent Server. The guide also explains how to connect an Ion Chef Instrument to the internal Torrent Server in Ion S5, Ion GeneStudio S5, and Ion GeneStudio S5 Plus sequencers. When you connect to a Torrent Server, you can use Torrent Suite Software to plan and monitor instrument runs, review run reports, and download run results from the connected instruments. You can use the following types of network connections to connect a Ion Chef Instrument to a Torrent Server or a Torrent Server that is embedded in an Ion GeneStudio S5 Sequencer: A local area network (LAN) by DHCP IP connection A direct connection A local area network (LAN) by Static IP connection If you add multiple Torrent Servers to a network, you can configure connections between servers, if you use Torrent Suite Software 5.6 and later. Connections that use an Ion Mesh configuration, enable you to do the following: Share Planned Runs with Torrent Servers that are included in the Ion Mesh configuration. Track reagent and cartridge usage across Ion Chef Instrument flexible workflows from different Ion Chef Instruments connected to different Torrent Servers. View and monitor all runs of interest across multiple Torrent Servers on the screen in Torrent Suite Software. About the Torrent Server Torrent Server in this guide refers to the server component of the PGM, Ion Proton, Ion S5 XL, or Ion GeneStudio S5 Prime System. Torrent Suite Software is the software that resides on the Torrent Server. You can transfer Planned Runs between servers that use the same versions of Torrent Suite Software and are connected to the same subnet. The Ion S5, Ion GeneStudio S5, and Ion GeneStudio S5 Plus sequencers have embedded Torrent Servers and this guide describes how to target the sequencer IP addresses to form network connections. Ion Chef Instrument Network Connection User Guide 5

6 1 Chapter 1 Introduction Overview Networking guidelines and best practices Network setup workflow We recommend that you consult a network administrator before connecting the Ion Chef Instrument to your laboratory network. See the Ion Chef System Site Preparation Guide (Pub. No. MAN ) for more information about the Ion Chef Instrument networking requirements. Collect the required network information. q Connect the Ion Chef Instrument to the configured LAN port. q Configure the Ion Chef Instrument with the required information. q Monitor the Ion Chef Instrument to test the network connection. q Share Planned Runs and monitor consumables with Ion Mesh. Collect the required network information Before you begin the procedures in this guide, obtain the following information from your network administrator: Network policy for obtaining IP addresses (DHCP IP or static IP). If the network requires static IP addresses, obtain the static IP address, subnet mask, gateway address, and DNS server name for the LAN port. Note: For the Planned Run sharing and file transfer protocol (FTP) functionality to be successful, the subnet must be the same on all Torrent Servers and Ion Chef Instruments on the LAN. 6 Ion Chef Instrument Network Connection User Guide

7 2 Connections between Torrent Server and the Ion Chef Instrument This chapter describes how to connect the standalone Torrent Server component of the PGM, Ion Proton, Ion S5 XL, or Ion GeneStudio S5 Prime System to an Ion Chef Instrument. General requirements The following diagrams illustrate common network configurations to which an Ion Chef Instrument and Torrent Server might be added. See the Ion Chef System Site Preparation Guide (Pub. No. MAN ) for more information. Direct connection For a direct connection, the Ion Chef Instrument is connected directly to the Torrent Server. 1 1 Network Ion Chef Instrument 3 Torrent Server Ion Chef Instrument Network Connection User Guide 7

8 2 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument General requirements DHCP IP In a DHCP IP network, the Ion Chef Instrument and the Torrent Server connect to the network DHCP Server 2 Network Ion Chef Instrument 4 Torrent Server Static IP In a static IP network, the Ion Chef Instrument and the Torrent Server are connected to the network. 1 1 Network Ion Chef Instrument 3 Torrent Server To share Planned Runs and successful FTP functionality, the subnet must be the same for all Torrent Servers and Ion Chef Instruments on the LAN. 8 Ion Chef Instrument Network Connection User Guide

9 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument Add the Torrent Server IP address 2 Add the Torrent Server IP address The first time that an instrument is connected to a Torrent Server, or any time the connection configuration is changed, for example from a direct connection to a connection over a network, you must add the IP address of the server to the instrument. To add the Torrent Server IP address from the Ion Chef Instrument. After you complete this procedure the IP address is available to select on the Ion Chef Instrument. 1. Sign in to Torrent Suite Software. 2. Click (Settings)4Services. 3. Record the server IP address that is displayed on the System Services screen. Later you will enter the IP address on the instrument screen. 4. Return to the Ion Chef Instrument user interface, then tap Manage. The Manage Connections dialog opens. Ion Chef Instrument Network Connection User Guide 9

10 2 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument Edit the Torrent Server firewall tables to allow the LAN port IP 5. Enter the server IP address or Torrent Server host name in the Server field and repeat for Update site. Click Save. The Torrent Server host name or IP address is linked to the Ion Chef Instrument. Edit the Torrent Server firewall tables to allow the LAN port IP When connecting through a LAN, the Torrent Server firewall settings need to be modified for both DHCP and Static LAN connections. Note: When directly connected, instruments are on the x.x subnet from which all traffic is enabled, so modification of firewall settings is not required. Any exception would mean that editing the local firewall tables to include the LAN IP is required. Also, if the Ion Chef Instrument is getting a DHCP address from your network, you need to create an exception for the entire DHCP subnet. Create a firewall exception for the Torrent Server Consult your system administrator or Thermo Fisher Field Service Engineer (FSE) or Field Application Specialist (FAS) for this procedure. On the Torrent Server, edit the /etc/iptables.custom file and add a firewall rule to allow all traffic from the IP address of the Ion Chef Instrument. (This is a blank file initially and is intended for custom firewall configuration.) IMPORTANT! Only edit the /etc/iptables.custom file and not /etc/iptables.rules, because /etc/iptables.rules is overwritten when software is updated. 1. Enter sudo^nano^/etc/iptables.custom, then add the three lines below: *filter -A INPUT -s xxx.xxx.xxx.xxx -j ACCEPT COMMIT Note: -A INPUT -s xxx.xxx.xxx.xxx -j ACCEPT adds the rule to the format. Enter the IP address of the Ion Chef instrument in place of "xxx.xxx.xxx.xxx". When finished editing, save changes by pressing CTRL + O and CTRL + X. 10 Ion Chef Instrument Network Connection User Guide

11 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument Connect directly to the Torrent Server 2 2. After this command, manually apply the change: sudo^iptables-restore^--noflush^<^/etc/iptables.custom Note: You can also list the firewall rules with: sudo^iptables-l. If this command returns with no output, it means it succeeded. 3. If the Ion Chef Instrument is getting a DHCP address from your network, create the exception for the entire DHCP subnet. The example below assumes that the DHCP address for the Ion Chef Instrument is and the DHCP subnet range is to Add these three lines: *filter -A INPUT -s /24 -j ACCEPT COMMIT 4. Restart the server. 5. (Optional) If you need to make corrections, repeat this process and completely drop all rules and start fresh. Enter the following: sudo^iptables-f sudo^iptables-restore^<^/etc/iptables.rules sudo^iptables-restore--noflush^</^etc/iptables.custom Removing a rule from either of these files would remove it from the active firewall rules, because the iptables-restore command does not overwrite rules, it adds them to all active rules. Connect directly to the Torrent Server You can directly connect the Torrent Server to the Ion Chef Instrument. 1. Verify Torrent Server connection to Ion Chef Instrument. a. Plug the connector from the Ion Chef Instrument directly into one of the available ports on the back of the server. The Ion Chef Instrument is assigned a default IP address, for example , based on the Torrent Server port you connect to. Ion Chef Instrument Network Connection User Guide 11

12 2 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument Connect directly to the Torrent Server b. On the Home screen, tap Settings4Instrument Settings4About4About Instrument, then verify the IP address for the server connection on the Ion Chef Instrument. 2. Connect the Ion Chef Instrument. a. On the Home screen, tap Settings4 Torrent Server. b. Verify that the server settings are correct. Ensure that the Torrent Suite Software password is correct. The Torrent Suite Software URL is the IP address of the port you connected to on the back of the server ( x.1). The server and instrument are now connected. 12 Ion Chef Instrument Network Connection User Guide

13 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument Connect by network to the Torrent Server 2 3. To set the password, tap Manage, then enter the password to the ionadmin account password on the selected Torrent Server. 4. Tap Save. The Torrent Server is directly connected to the Ion Chef Instrument, an icon ( ) on the lower left side of the home screen appears. You can now use Ion Mesh to share Planned Runs and monitor consumables. Connect by network to the Torrent Server To connect to the Torrent Server through the company LAN, you need to target ServerPortIP (Eth1), see Add the Torrent Server IP address on page 9 for more information. When the LAN is set to DHCP, the IP, Gateway, Netmask, and DNS Name Servers populate automatically. You will also need to modify the Torrent Server firewall settings. See Edit the Torrent Server firewall tables to allow the LAN port IP on page 10 for more information. 1. On the Home screen, tap Settings4Instrument Settings4Network Configuration. 2. Select the appropriate IP configuration: Option DHCP Description Dynamic Host Configuration Protocol is a client/server protocol that automatically provides an Internet Protocol (IP) host with its IP address and other related configuration information such as the subnet mask and the gateway. Static IP Static Internet Protocol (IP) address is a permanent number assigned to a computer by an Internet Service Provider (ISP). Ion Chef Instrument Network Connection User Guide 13

14 2 Chapter 2 Connections between Torrent Server and the Ion Chef Instrument Connect by network to the Torrent Server 3. Ensure that the information is correct, then tap Done. The Torrent Server is connected to the company LAN, an icon ( ) on the lower left side of the home screen appears. You can now use Ion Mesh to share Planned Runs and monitor consumables. 14 Ion Chef Instrument Network Connection User Guide

15 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument This chapter describes how to connect an Ion Chef Instrument to the internal Torrent Server component in an Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer. General requirements The following diagrams illustrate common network configurations to which an Ion Chef Instrument and a Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an internal Torrent Server might be added. See the Ion Chef System Site Preparation Guide (Pub. No. MAN ) for more information. Direct connection For a direct connection, the Ion Chef Instrument is connected directly to an Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded server. 1 1 Network Ion Chef Instrument 3 Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded server Ion Chef Instrument Network Connection User Guide 15

16 3 Chapter 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument General requirements DHCP IP In a DHCP IP network, the Ion Chef Instrument and the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded server connect to the DHCP IP network DHCP Server 2 Network Ion Chef Instrument 4 Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded server Static IP In a static IP network, the Ion Chef Instrument and the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with embedded server are connected to the network. 1 1 Network Ion Chef Instrument 3 Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded server To share Planned Runs and successful FTP functionality, the subnet must be the same for all sequencers and Ion Chef Instruments on the LAN. 16 Ion Chef Instrument Network Connection User Guide

17 Chapter 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument Add the sequencer IP address 3 Add the sequencer IP address The first time that an instrument is connected to an Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded Torrent Server or any time the connection configuration is changed, for example from a direct connection to a connection over a network, you must add the IP address of the sequencer to the instrument. After you complete this procedure the IP address is available to select on the Ion Chef Instrument. 1. If you are connecting the Ion Chef Instrument to a Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded Torrent Server over a network, click Settings4Instrument Settings4About on the sequencer and locate the target IP address displayed on the sequencer user interface screen. 2. Return to the Ion Chef Instrument user interface, then tap Manage. The Manage Connections dialog opens. 3. Enter the sequencer IP address or host name in the Server field and repeat for Update site. Click Save. The sequencer IP address is linked to the Ion Chef Instrument. Ion Chef Instrument Network Connection User Guide 17

18 3 Chapter 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument Edit the Torrent Server firewall tables to allow the LAN port IP Edit the Torrent Server firewall tables to allow the LAN port IP When connecting through a LAN, the firewall settings of an Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded Torrent Server need to be modified for both DHCP and Static LAN connections. Note: When directly connected, instruments are on the x.x subnet from which all traffic is enabled, so modification of firewall settings is not required. Any exception would mean that editing the local firewall tables to include the LAN IP is required. Also, if the Ion Chef Instrument is getting a DHCP address from your network, you need to create an exception for the entire DHCP subnet. Create a firewall exception for the Torrent Server Consult your system administrator or Thermo Fisher Field Service Engineer (FSE) or Field Application Specialist (FAS) for this procedure. On the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded Torrent Server, edit the /etc/iptables.custom file and add a firewall rule to allow all traffic from the IP address of the Ion Chef Instrument. (This is a blank file initially and is intended for custom firewall configuration.) IMPORTANT! Only edit the /etc/iptables.custom file and not /etc/iptables.rules, because /etc/iptables.rules is overwritten when software is updated. 1. Enter sudo^nano^/etc/iptables.custom, then add the three lines below: *filter -A INPUT -s xxx.xxx.xxx.xxx -j ACCEPT COMMIT Note: -A INPUT -s xxx.xxx.xxx.xxx -j ACCEPT adds the rule to the format. Enter the IP address of the Ion Chef instrument in place of "xxx.xxx.xxx.xxx". When finished editing, save changes by pressing CTRL + O and CTRL + X. 2. After this command, manually apply the change: sudo^iptables-restore^--noflush^<^/etc/iptables.custom Note: You can also list the firewall rules with: sudo^iptables-l. If this command returns with no output, it means it succeeded. 3. If the Ion Chef Instrument is getting a DHCP address from your network, create the exception for the entire DHCP subnet. The example below assumes that the DHCP address for the Ion Chef Instrument is and the DHCP subnet range is to Add these three lines: *filter -A INPUT -s /24 -j ACCEPT COMMIT 18 Ion Chef Instrument Network Connection User Guide

19 Chapter 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument Connect directly to the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer 3 4. Restart the server. 5. (Optional) If you need to make corrections, repeat this process and completely drop all rules and start fresh. Enter the following: sudo^iptables-f sudo^iptables-restore^<^/etc/iptables.rules sudo^iptables-restore--noflush^</^etc/iptables.custom Removing a rule from either of these files would remove it from the active firewall rules, because the iptables-restore command does not overwrite rules, it adds them to all active rules. Connect directly to the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer The Ion S5, Ion GeneStudio S5, and Ion GeneStudio S5 Plus sequencers include a built-in server. You can connect an Ion Chef Instrument and the sequencer directly and plan runs for both instruments through the Torrent Suite Software. Before you can complete this procedure the Ion Chef Instrument and the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer must be connected directly through a cable connection. See the your site administrator or the Ion Chef System Site Preparation Guide (Pub. No. MAN ) for more information about this connection and Ion Chef Instrument networking requirements. 1. Connect the Ion Chef Instrument directly to the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer, then click Settings4Instrument settings4torrent Server. 2. Record the following information from the screen: Setting Example Value Update site Server Username (browser) Password (browser) Username (ftp) Password (ftp) ionadmin ionadmin ionguest ionguest 3. To connect the instruments for the first time, tap Manage on the Ion Chef Instrument, then enter the recorded information in the Manage Connections dialog, and click Save. The Ion Chef Instrument is now connected to the Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer, an icon ( ) on the lower left side of the home screen appears. You can now use Ion Mesh to share Planned Runs and monitor consumables. Ion Chef Instrument Network Connection User Guide 19

20 3 Chapter 3 Connections between the Ion S5 or Ion GeneStudio S5 Systems and the Ion Chef Instrument Connect by network to the Ion S5 or Ion GeneStudio S5 Sequencer Connect by network to the Ion S5 or Ion GeneStudio S5 Sequencer To connect to an Ion Chef Instrument to an Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer with an embedded Torrent Server through the company LAN, you need to target ServerPortIP (Eth1), see Add the sequencer IP address on page 17 for more information. When the LAN is set to DHCP, the IP, Gateway, Netmask, and DNS Name Servers populate automatically. You will also need to modify the Ion S5 or Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer firewall settings. See Edit the Torrent Server firewall tables to allow the LAN port IP on page 18 for more information. 1. On the Home screen, tap Settings4Instrument Settings4Network Configuration. 2. Select the appropriate IP configuration: Option DHCP Description Dynamic Host Configuration Protocol is a client/server protocol that automatically provides an Internet Protocol (IP) host with its IP address and other related configuration information such as the subnet mask and the gateway. Static IP Static Internet Protocol (IP) address is a permanent number assigned to a computer by an Internet Service Provider (ISP). 3. Ensure that the information is correct, then tap Done. The Ion S5, Ion GeneStudio S5, or Ion GeneStudio S5 Plus Sequencer is connected to the company LAN, an icon ( ) on the lower left side of the home screen appears. You can now use Ion Mesh to share Planned Runs and monitor consumables. 20 Ion Chef Instrument Network Connection User Guide

21 4 Monitor runs, share Planned Runs, and manage consumables with Ion Mesh The Ion Mesh network capability was introduced in Torrent Suite Software 5.6 to make Planned Run sharing and run monitoring easier. It also enables consumables tracking. Set up Ion Mesh Ion Mesh is a feature in Torrent Suite Software that allows you to form a connection between Torrent Servers. Note: Torrent Servers must be running the same software version. When using Ion Mesh, you can: View all runs of interest across multiple Torrent Servers on the Completed Runs and Reports screen. Transfer Planned Runs between sequencers that are connected to different Torrent Servers. Track reagent and cartridge usage across Ion Chef Instrument flexible workflows from different Ion Chef Instruments connected to different Torrent Servers. Follow these steps to connect (link) your Torrent Server to another Torrent Server. 1. In any tab, click (Settings), then click Ion Mesh. Ion Chef Instrument Network Connection User Guide 21

22 4 Chapter 4 Monitor runs, share Planned Runs, and manage consumables with Ion Mesh View sequencing runs on multiple sequencers with Ion Mesh 2. Select one of the following options. Option Description Link a selected Torrent Server. Select a Torrent Server from the dropdown list menu, then click Link Selected TS. Manually link a Torrent Server. Click Link TS Manually. 3. Enter the following information in the Setup Mesh Computer dialog. Parameter Definition Hostname/Address Host name or address of the server. Note: If linking a selected Torrent Server, this field is automatically populated and cannot be edited. Nickname Username Password A common name that is assigned to the Torrent Server. Your username. Your password. 4. Click Setup. The linked and enabled Torrent Servers are available to be used in the Planned Runs and the data from the linked and enabled Torrent Servers can be viewed on the same Data page. View sequencing runs on multiple sequencers with Ion Mesh Before viewing runs on multiple servers, you must first connect the servers using Ion Mesh. See Set up Ion Mesh on page 21 for more information. 1. In the Data tab, click Completed Runs and Reports. 2. Select one or more Torrent Servers that you want to view data from in the Server dropdown list. Notice that the server name is now reflected in the Run Name column. Your results across multiple sequencers are ready for your review. 22 Ion Chef Instrument Network Connection User Guide

23 Chapter 4 Monitor runs, share Planned Runs, and manage consumables with Ion Mesh Transfer a Planned Run to a Torrent Server with Ion Mesh 4 Transfer a Planned Run to a Torrent Server with Ion Mesh You can transfer Planned Runs that are created on one Torrent Server and transfer them to another Torrent Server with Ion Mesh. This is useful if the sequencer connected directly to the origin Torrent Server is offline or busy. Before Planned Run transfer, first connect the Torrent Servers with Ion Mesh. See Set up Ion Mesh on page 21 for more information. Note: The Torrent Servers must have the same version of Torrent Suite Software installed in order for this Planned Run transfer to be successful. 1. Sign in to Torrent Suite Software on the origin Torrent Server. 2. In the Plan tab, click Planned Runs. 3. Find the row of the Planned Run you want to transfer, then click (Actions)4Transfer. 4. Select the Torrent Server that you want to receive the Planned Run. 5. A confirmation dialog appears. Check the information, then click Transfer. Note: You can no longer access this Planned Run on the origin server after it has transferred. A status appears in the dialog with the results of the transfer. A green box lists the Planned Runs that are successfully transferred. A red box lists any failed Planned Run transfers. Ion Chef Instrument Network Connection User Guide 23

24 4 Chapter 4 Monitor runs, share Planned Runs, and manage consumables with Ion Mesh Remove a server from an Ion Mesh configuration Remove a server from an Ion Mesh configuration You can permanently remove a Torrent Server from an Ion Mesh configuration. 1. In any tab, click (Settings), then click Ion Mesh. 2. In the Configure Mesh dialog box, click (Actions) in the row of the server you want to unlink, then click Unlink. 3. On the confirmation window, click OK. Note: Unlinking a Torrent Server permanently removes that server from Ion Mesh. The Torrent Server is removed from the Ion Mesh configuration. Change server nickname in an Ion Mesh configuration You can edit the nickname of a Torrent Server linked by Ion Mesh. 1. In any tab, click (Settings), then click Ion Mesh. 2. In the Configure Mesh dialog box, click (Actions) in the row of the server you want to edit, then click Edit. 3. Change the nickname of the linked Torrent Server, then click Setup. The Torrent Server nickname is changed. Enable or disable an Ion Mesh connection You can enable or disable any Torrent Server that is connected through an Ion Mesh configuration. The Torrent Server must be included in the configuration before you do this procedure. In any tab, click (Settings), then click Ion Mesh. Option Enable a Torrent Server Disable a Torrent Server Action To enable a Torrent Server: select the checkbox in the Enable column of the Configure Mesh table in the row of the server. To disable a Torrent Server: deselect the checkbox in the Enable column of the Configure Mesh table in the row of the server. Note: Disabling a Torrent Server hides that server from Planned Runs actions and Data menus so the unlinked server cannot be used by the Torrent Suite Software. 24 Ion Chef Instrument Network Connection User Guide

25 Chapter 4 Monitor runs, share Planned Runs, and manage consumables with Ion Mesh Track Ion Chef flexible workflows for Ion 550 chips with Ion Mesh 4 Track Ion Chef flexible workflows for Ion 550 chips with Ion Mesh To use the flexible workflow feature and enable cartridge use tracking between Torrent Servers across multiple Ion Chef instruments, Torrent Servers must be linked in an Ion Mesh configuration. For further information on how to link Torrent Servers in an Ion Mesh setup, see Set up Ion Mesh on page 21. For more information on configuring a flexible workflow on the Ion Chef instrument, see the Ion 550 Kit Chef User Guide (Pub No. MAN ). IMPORTANT! If you have Torrent Servers that are connected in an Ion Mesh configuration, and the connection to one Torrent Server is disrupted or lost, cartridge tracking between servers is disabled. In this situation, an error message appears if you attempt to start a run. The run is not allowed to start because cartridge use status is not trackable. Ion Mesh communication must be restored to verify that cartridge use status before the run can start. 1. In the Data tab, click Completed Runs & Reports. 2. Click a completed report. 3. In the run report, scroll down to Details section, then select Chef Summary and S5 Consumable Summary tabs to track the cartridge use. Ion Chef Instrument Network Connection User Guide 25

26 Related documentation Document Publication number Description Ion PGM System Site Preparation Guide MAN Describes the Ion PGM System hardware and software and provides information on preparing, maintaining, and troubleshooting the system. Ion Proton System Site Preparation Guide Describes the Ion Proton System hardware and software and provides information on preparing, maintaining, and troubleshooting the system. Ion S5 and Ion S5 XL System Site Preparation Guide Ion GeneStudio S5 System Site Preparation Guide MAN MAN Describes the Ion S5 Systems software and provides procedures for common tasks. Describes the Ion GeneStudio S5 Systems hardware and software and provides information on preparing, maintaining, and troubleshooting the system. Customer and technical support Visit thermofisher.com/support for the latest service and support information. Worldwide contact telephone numbers Product support information Product FAQs Software, patches, and updates Training for many applications and instruments Order and web support 26 Ion Chef Instrument Network Connection User Guide

27 Related documentation Limited product warranty Product documentation User guides, manuals, and protocols Certificates of Analysis Safety Data Sheets (SDSs; also known as MSDSs) Note: For SDSs for reagents and chemicals from other manufacturers, contact the manufacturer. Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale at If you have any questions, please contact Life Technologies at Ion Chef Instrument Network Connection User Guide 27

28 thermofisher.com/support thermofisher.com/askaquestion thermofisher.com 2 October 2018