THERMAL CYCLER PCRSG-500

Transcription

1 Operation Manual for THERMAL CYCLER PCRSG-500 PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3, Hagavish st. Israel Tel: , Fax: mrc@mrclab.com MRC.VER

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3 34 1. Maintenance Troubleshooting 36 Appendix B Instrument Guarantee Guarantee Content Guarantee Scope Page 2 of

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5 2 Safety notice Please read the manual carefully before operating the instrument, the wrong operation may be hazardous to the operator or the instruments itself. 1 Operating safety The power cord for the instrument should be grounded reliably. The instrument adopts three-core grounding plug, and should be connected with appropriate power socket. Before connecting to the power, you must make sure that the voltage and power load of the socket is in accordance with the capacity on the instrument's nameplate. Please change the power cord with the same type if the skin of the cord is found to be broken. Nothing is allowed to be place on the power cord and the cord shall not be placed in the area that may be easy approaching by people. Be sure to hold the plug tightly while inserting, pulling of the cord directly is forbidden. While the instrument is in operation, the sample plate block and the heat lid will produce high temperature, so no part of human body is allowed to touch the metal block or the heat lid while the instrument is in operation in case of any scalding. For the good ventilation, no articles are allowed to be placed within 30cm around the instrument. 2 Maintenance Safety The block and the heat lid of the instrument need regular cleaning to guarantee the precision of the experiment. Before cleaning, the sample plate block is suggested to be taken out for soft scrubbing by the clean soft cloth stained with the pure alcohol in appropriate amount. No corrosive cleaning agent is allowed for use and dripping the cleaning agent into the taper hole is forbidden in case of any damage to the instrument. And the sample plate block should be put back into the instrument after cleaning. Please cut off the power at once and stop the experiment and then immediately Page 4 of

6 contact the distributor or the professional maintenance personnel in case of any occurrence of the following situations: Any reagent, rain water or other liquid enters into the instrument. The instrument falls from a high place or the instrument shell is damaged. The operating instrument produces the abnormal noise or the pungent odor. The features of the instrument change apparently, such as failures of startup or shutdown or failure to run user program, etc. Notice: For your personal safety and the normal operation of the instrument, please do not disassemble the instrument by yourself. Any such behavior may endanger your safety and deprive your guarantee right of the instrument. Page 5 of

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8 (6)Control panel (7)Screen (8) Key pad (9) Power switch (10) Power socket (11) Fuse socket (12) Network interface (13) USB interface (14) JTEG interface (15) Shell (16) Ventilation window (17) LOGO (18) Lid wrench (19) Block selecting knob (20) Block selecting indicator 2.2 Lid-opening angle This type of instrument takes a leading role in adopting the hinge joint technology, enabling the upper lid of the instrument to stay at any angle according to requirement and solving such problems brought by the traditional method of relying on spring elasticity to open the lid as unreliability, instability, large inertia and short life etc. Pull the wrench to push the lid backward Stop at any angle according to requirements As indicated in the above two figures, when the wrench is raised the buckle will be released, and then the user can set the upper lid at any angle according to their requirement. Humanistic structure endows the product more beautiful and practical characteristics, and guarantees the safety and convenient operation. Notice: According to the instrument characteristics, the open angle scope between upper lid and the shell is 0 ~ 90, please keep it within the scope to prevent any damage to the instrument. Page 7 of

9 2.3 Hot Lid positions Frequently, experiments need different reaction tubes, that is, use different blocks to match the tubes. The design of the heat lid gear positions realize the heat lid to match different sample plate blocks just by turning the knob to the appropriate position. As the above figures indicate that the mark of 0.5, 0.2,384 (clockwise) on the panel respectively corresponds to the gear position of tube 0.5ml, 0.2ml and 384sample plate blocks. If you need the 0.2ml tube for experiment, you only need to change the block with 0.2ml block (See 2.4 Module for details), then switch the gear to the mark of 0.2, and then you can start your experiment. The design abandons the traditional practice of changing heat lid/or even instrument. Notice: According to characteristics of the instrument, the turning range of the switch is between 0.5 to 384, do not try to turn the switch out of this range. 2.4 Module For the need of users to use tubes of different specifications for experiment in the same instrument, and for the convenience of users to change relevant parts directly and effectively, the instrument adopts the portable design with auto-lock function to make the instrument meet the experiments of different tubes on the condition of changing least parts, which fully reflects the humanistic design of the instrument. Page 8 of

10 Block components (1) Handle (2) Magnetic steel (3) Frame (4) Block (5) Heat sink The above figure indicates that the integrated block parts design of the instrument greatly improves operational convenience no matter in installation or replacement, and the overall dimension, assembly process and installation method of all the blocks of all specifications are the same (The block parts of different specifications differ with blocks corresponding to tubes of different specifications). And this guarantees that users can change or install the block in a fast and high-efficient way only by choosing the block with specification needed. Figure 1 show how to change the block unit. When changing the block, the user shall pull the handle upward, when the handle and the shell are in a vertical state, the block will be unlocked, and then the user can easily take out the block part by pulling the handle vertically. Figure 2 show how to insert the block, when the user confirm the block part of certain specifications needed, the user only needs to hold the block s handle. Vertically insert the block in position, then put the handle down to complete the operation. Page 9 of

11 Step 1: Move the handle to the state vertical to the shell to make the block parts unlocked. (Figure 3) Step 2: Move the block parts upward slightly, and lay it in a safe place after the block parts are completely off the shell. (Figure 4) Step 3: Choose the block parts of specifications needed and gently put them into the shell vertically. Step 4: After the block parts completely contact the shell, press back the handle Page 10 of

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14 Style of temperature control: block; tube calculated Maximum number of program units: 1000 Maximum number of cycles: 255 Graphical interfaces:5.7inch,640*480pels LCD Communication interface: USB2.0 RS232 RJ45 Exterior size: 380mm(L)*270mm(W)*250mm(H) Net weight: 7.8kg Page 13 of

15 Chapter 3 Instructions Optical disk contains ActiveSync software, oprating environment: Win9xMeNT2000XP2003;VistaWin7 System is Windows Mobile. 3.1 Program installation Step 1 Install ActiveSync software in PC. (Windows Mobile of Vista Win7 is updated version of ActiveSync) Step 2 Connect PC and PCR by USB cable. Step 3 Right click My Computer Properties Hardware Device Manager Windows CE USB Devices right click USB Device Update Driver,to install USB driver.(figure 3-1, 3-2) 3-1 Page 14 of

16 3-2 Step 4 Click Browse and find out the file containing USB driver.(figure ) Page 15 of

17 Step 5 After selecting No in New Partnership, (Figure 3-5)PC will be connected to PCR.(Figure 3-6) Step 6 Nandflash appears in PC, double click to enter. Step 7 In Nandflash, replace old TempertureCtrl with new TemperatureCtrl and TCtrl, then software upgrades successfully. Step 8 Decompress installation package of upper computer in PC,click install and select language. (Figure 3-7) Page 16 of

18 3-7 Step 9 Click to select PCR program(figure 3-8) 3-8 Step 10 Select installation path.(figure 3-9) 3-9 Step 11 Confirm installation file name create desk top icon click to complete installation.(figure 3-10) Page 17 of

19 Program upgrade Step1. Double click master computer icon interface. (Figure 3-11) to enter program operation 3-11 Step2. While connect to Thermal Cycler, dialog box of update version will appear. (Figure 3-12) Select Yes to start upgrading program.(figure 3-13) 3-12 Page 18 of

20 3-13 Step3. Dialog box appears when upgrade completed, (Figure 3-14), click OK and restart PCR, then upgrade completed Startup interface After connecting related power, turn on the power switch of the instrument,it will produces the sound of tick-tack, which means the instrument is in operation,.the screen will display the startup interface (Figure 3-15), and start automatic systematic inspection,after entering the stand-by interface (Figure 3-16),it means the instrument is free for experimental operations now. If the screen is dark or any other abnormal phenomena occur while turn on the instrument, please cut off the power immediately and refer to Chapter 5 for maintenance or contact the distributor/the supplier for settlement. Page 19 of

21 Files In the" Main Menu" select "File" submenu to get into "File" interface, choose the "Open " or "Create ".(Figure 3-17) 3-17 Page 20 of

22 Notice: "Create -- create new files. "Open -- open saved files. Specific program file setting showed in chapter Setting In the" Main Menu" select "Set" submenu to get into "Operation Parameters Setting" interface. (Figure 3-18) 3-18 Notice: The Heating/Cooling rate range is 1.0 /s~4.0 /s. The temperature range of hypothermic preservation can as low as 0.0.Temperature control mode: sample block control and tube control. 3.6 Hot Lid In the "Main Menu" select "Hot Lid" submenu to get into "Hot Lid Function Setting" interface. (Figure 3-19) 3-19 Notice: the hot lid temperature range is: 20 ~110. Page 21 of

23 3.7 Gradient In the" Main Menu" select Grads" submenu to get into the "Gradient View" interface. (Figure 3-20) 3-20 Notice: Central temperature range: 31 ~99, grads range:1 ~15,the algebra sum of the central temperature and the grads scale range:30 ~ 100.Operator can view the temperature of 12 corresponding block rows. The Gradient graph cannot used if user put in the 384module since the gradient function of the 384module is not available. 3.8 Network In the" Main Menu" select "Network" submenu to get into the "Network Setting" interface. (Figure 3-21) 3-21 Notice: Network setting is mainly setting the IP address which set up network Page 22 of

24 3.9 Log connection to the host computer, and the port number setting. Port number 8008 is a fixed value. IP address must be consistent with the host computer. In the "Main Menu" Select "Log" submenu to get into "Log View" interface. (Figure 3-22) 3-22 Notice: If the numbers of all currently created files is below 200, all the files information will be displayed in this window. If the number of files is above 200, the most recently created 200 files information will be displayed. This information include the file names, running time, and created date, etc System In the" Main Menu" select "System" submenu to get into the "System Setting" interface. (Figure 3-23) 3-23 Notice: Press the Arrow key to choose the system date, alert sound, file list arrangement type, etc. for setting. System date and time setting should be Page 23 of

25 inputted with the Numerical keys on the machine Help 3-24) In the "Main Menu" select "Help" submenu to get into "Help" interface. (Figure 3-24 Notice: The TM key showed on the interface is for checking the TM value. If the user inputs the value forbidden by the system while setting the parameter, the system will choose the default value, and the forbidden value will not be allowed. The version of this instrument is V2.0,showed in the Help interface. Page 24 of

26 Chapter4 Programming on Thermal Cycler Notice: Select the Close key will cause the system be back to the Windows CE system interface. 4.1 Create Files (The following pictures for reference only) The following is a sample(30 cycles, 55 :90s,0 :30s,72 :3mins) of programing. (Figure 4-1) Temp Edit Pull-down Menu Cycle Edit Pull-down Menu Close key Back key Right/Left selection key Running Key 4-1 Step 1: Select the "Create" after entering the "File" menu, (Figure 4-2), then input the file name (Figure 4-3).File name can be inputted with Numerical keys on the machine or keypad in the interface. After that close the keypad and click the "Confirm" key to confirm. After creating a new file you can choose to setting password or not. If setting password, then click the "Confirm" key after input password. (Figure 4-3) Page 25 of

27 Step 2: Get into the "Temp Edit and "Cycle Edit" interface for editing, click the "Temp Edit" pull-down menu and choose the "Edit Temp Point" to edit temperature. If you need to increase temperature, click the "Temp Edit" pull-down menu and select "Add Temp Point" for editing. Select "Del. Temp Point to remove temp point if there is extra point. (Figure 4-4) Page 26 of

28 4-4 Step 3: In the "Temp Edit and" Cycle Edit" interface, use the Right/Left selection key or Arrow keys to move the cursor to the temp point which will be the temp cycle starting point, click the "Cycle Edit" and select the "Cycle Start Point. Then move to the one which will be the cycle ending point, click the "Cycle Edit" and select the "Cycle End Point". (Figure 4-5) 4-5 Step 4: Click the "Run" key to start running files. (Figure4-6) You can choose the Run, Pause, Stop keys to operate the program. Page 27 of

29 4.2 Open Files 4-6 Click the "File" on the main menu interface to get into the "File interface (Figure 4-7), select "Open " to get into the file list interface(figure4-8),then open the file what you want to start running Page 28 of

30 Chapter5 Programming on Master Computer Notice:Conect to Thermal Cycler with network cable. 1 Interface introduction Double click master computer icon to enter operation interface. (Figure 5-1) Interface is composed of menu bar, tool bar, PCR machine area, program area, implementation view etc. 5-1 Key Name Add PCR Edit PCR Function add PCR instrument edit IP address of PCR(Not Port) Page 29 of

31 Delete PCR Connect Disconnect New Edit Delete Run Pause Stop Cancel delete PCR instrument connect to Thermal Cycler disconnect from Thermal Cycler create PCR program edit PCR program delete PCR program run PCR program pause running PCR program stop running PCR program cancel operation Gradient table view gradient table TM TM calculation 2 Create Files Step 1: Click Add button and select PCR instrument. Input PCR instrument name, IP address, IP port(fixed value, 8008), then click OK.(Figure 5-2) Page 30 of

32 5-2 Step 2: click Connect button to connect to Thermal Cycler. Step 3: click New button, dialog box of programming will appear. (Figure 5-3) Users can edit program name, creater name and set password. System can provide default program, however, users can edit via program parameter area at the left of the interface according to the requirements. Then click Save button to save program if confirmed. 5-3 Page 31 of

33 Step 4: Click Run button in the tool bar to run program. Double click Running view to view the status of running program.(figure 5-4) 5-4 Page 32 of

34 Appendix A Maintenance and Troubleshooting 1 Maintenance The instrument shall be stored in the dry and ventilating environment, keep away from direct sunlight or humidity or shadiness. Clean the wells of the block regularly in case the residue left by the experiment affect the accuracy of the experiment, the corrosive solvent or cleaning agent is forbidden, and cleaned by the cotton swab stained with water free alcohol in appropriate amount is suggested, and then an compressed air is used to clean each hole. Cotton cloth or non-woven stained with appropriate pure water shall be used for cleaning if there is dirt or dust on the surface of the instrument, organic solvent, alcohol, strong acid, strong alkaline or other corrosive cleaning agent is not allowed in case of any damage to the instrument surface. After the experiment, check the instrument and turn off the power, remove all the residues left by the experiment such as the water, reaction solution and impurity in and out of the instrument to keep the instrument clean. The protective cover shall be used to cover the instrument if it will not be used for a long time. 2 Troubleshooting 2.1 Bad experiment analysis With the use of this instrument, if the experimental result is poor, then the following problems maybe exist (Only for reference): A. The reaction article is wrong, or the quantity is inadequate, or the purity is not enough or the slight mononuclear chain area is incorrect. B. The denatured temperature is too high or too low, so the user can add or reduce time according to the reaction amount. C. The annealing temperature is too high or too low. Page 34 of

35 D. The reactant concentration is too high or too low. E. The preparation process is not specially treated. F. The time and temperature value in the programming is not suitable. G. The temperature of the sample or sample electrode is a little low, whereas the base temperature is a little high. H. The user shall check if the reaction tube is placed well, if not, little mineral oil can be used to coat the cone hole to increase heat conduction. I. Deviation may occur to temperature control if it is not used for a long time, if so the user may require the manufacturer to readjust the temperature control, any personal testing or readjustment of the temperature control is not allowed. 2.2 Problem reason analysis and Corresponding solving method No. Phenomenon Analysis Solutions 1 The screen is The power is not connected Connect the power dark and there The fuse is burnout Change for a new is no tick fuse(250v 10A sound after Ф5x20) opening the The switch is damaged Change the switch power others Contact the supplier 2 The screen displays wrong box with the sound of Tick 3 The slow temp down speed of the block or failure of down 4 The slow temp up speed of the block or failure of up 5 Failure of heating or cooling of the block 6 Failure of heating the heat lid 7 The screen displays abnormal character 1.The open circuit or short circuit of the cooling block sensor 2 The open circuit or short circuit of the heat lid sensor 3 The open circuit or short circuit of the radiator 4 others Block of vent The cryogenic chip is damaged or the ventilator stop working. Cryogenic chip is damaged Temperature sensor is damaged Cryogenic chip is damaged Heating film is damaged Temperature sensor is damaged The link line is loose Bad contact of the chip The chip is damaged or manufacturer Contact the supplier or manufacturer Remove the clogs Contact the supplier or manufacturer Contact the supplier or manufacturer Contact the supplier or manufacturer Contact the supplier or manufacturer Contact the supplier or manufacturer Page 35 of

36 8 The press key of the panel does not work Button switch is damaged Contact the supplier or manufacturer Appendix B Instrument Guarantee 1 Guarantee Content a) The instrument, a common B-type instrument for indoor uses, is in accordance with the I-type of the standards of GB9706.1, and has passed CE security authentication. b) Within one month as of the date of the delivery, for any damage caused by materials or manufacturing happening to the instrument, we will change it.. c) Within 4 years as of the date of the delivery, for any damage caused by materials or manufacturing happening to the instrument, our company will provide the guarantee service. Within the warranty period, the company will repair or change the instrument proved to be defective. d) The user shall send the product to the maintenance department confirmed by our company. The user shall pay the expense occurred by transferring the instrument to the maintenance department. And the company shall pay the expense occurred by transferring the instrument back to the user. e) As to the repair beyond the warranty period, the company will charge the relevant maintenance fees. 2 Guarantee Scope a) Under normal working conditions, if any damage or default to the instrument is caused by materials, installation or other flaws of the instrument itself, our company shall provide the maintenance services according to the above-mentioned maintenance contents. b) If the user fails to operate according to the standard specified or uses it under the inappropriate conditions, or arbitrarily dismantles or repairs the instrument without authorization, then any damage or default thus occurred shall not be contained in the maintenance scope. Page 36 of