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2 MC-2302 N 6 -Benzyladenine, [benzyl ring- 14 C]- Lot A MW A) All chromatograms were run using the HPLC method described on the Product Data Sheet. Concentrations and volumes: Standard solution concentration was 0.5 mg/ml. N 6 -Benzyladenine, [benzyl ring- 14 C]- concentration was 52.0 µci/ml. Volume of standard alone injection was 1.0 µl Volume of N 6 -Benzyladenine, [benzyl ring- 14 C]- alone injection was 1.0 µl. Co-injection solution consisted of 1.0 µl N 6 -Benzyladenine, [benzyl ring- 14 C] µl standard. Volume of co-injection was 2.0 µl. Volume of blank injection was 1.0 µl. B) Mass spectrometry - Positive mode C) NMR
3 2/18/ :06 AM Chromatogram L:\int42300.PRM Page 1 of 2 Chromatogram Info: MC-2302 N6-Benzyladenine, [benzyl ring-14c]- Lot A M W File Name : L:\int42300 File Created : 2/18/ :55:30 AM Origin : Acquired, Acquisition started 3/11/2010 4:14:45 PM Acquired Date : 3/11/2010 4:54:44 PM Project : Test By : Administrator Method : Unit4-40minrun By : Administrator Description : Radiochemical trace of 14C material alone Created : 6/12/ :30 AM Modified : 2/18/ :06 AM Column : Detection : Radiochemical Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 4 Radio Voltage Time [min.]
4 2/18/ :06 AM Chromatogram L:\int42300.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 4 Radio) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
5 2/18/ :05 AM Chromatogram L:\int42300.PRM Page 1 of 2 Chromatogram Info: MC-2302 N6-Benzyladenine, [benzyl ring-14c]- Lot A M W File Name : L:\int42300 File Created : 2/18/ :55:30 AM Origin : Acquired, Acquisition started 3/11/2010 4:14:45 PM Acquired Date : 3/11/2010 4:54:44 PM Project : Test By : Administrator Method : Unit4-40minrun By : Administrator Description : UV trace of 14C material alone Created : 6/12/ :30 AM Modified : 2/18/ :59 AM Column : Detection : UV 254nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 4 UV Voltage Time [min.]
6 2/18/ :05 AM Chromatogram L:\int42300.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 4 UV) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Total Response Factor Resolution
7 2/18/ :07 AM Chromatogram L:\int42301.PRM Page 1 of 2 Chromatogram Info: MC-2302 N6-Benzyladenine, [benzyl ring-14c]- Lot A M W File Name : L:\int42301 File Created : 2/18/ :55:29 AM Origin : Acquired, Acquisition started 3/12/ :52:49 AM Acquired Date : 3/12/ :32:48 AM Project : Test By : Administrator Method : Unit4-40minrun By : Administrator Description : UV trace of standard alone Created : 6/12/ :30 AM Modified : 2/18/ :00 AM Column : Detection : UV 254nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 4 UV 300 Voltage Time [min.]
8 2/18/ :07 AM Chromatogram L:\int42301.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 4 UV) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
9 2/18/ :09 AM Chromatogram L:\int42302.PRM Page 1 of 2 Chromatogram Info: MC-2302 N6-Benzyladenine, [benzyl ring-14c]- Lot A M W File Name : L:\int42302 File Created : 2/18/ :55:29 AM Origin : Acquired, Acquisition started 3/12/ :11:49 PM Acquired Date : 3/12/ :51:48 PM Project : Test By : Administrator Method : Unit4-40minrun By : Administrator Description : Radiochemical trace of 14C material co-injected with standard Created : 6/12/ :30 AM Modified : 2/18/ :08 AM Column : Detection : Radiochemical Mobile Phase : Temperature : Flow Rate : Pressure : Note : 500 L:\ int Unit 4 Radio Voltage Time [min.]
10 2/18/ :09 AM Chromatogram L:\int42302.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 4 Radio) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
11 2/18/ :08 AM Chromatogram L:\int42302.PRM Page 1 of 2 Chromatogram Info: MC-2302 N6-Benzyladenine, [benzyl ring-14c]- Lot A M W File Name : L:\int42302 File Created : 2/18/ :55:29 AM Origin : Acquired, Acquisition started 3/12/ :11:49 PM Acquired Date : 3/12/ :51:48 PM Project : Test By : Administrator Method : Unit4-40minrun By : Administrator Description : UV trace of 14C material co-injected with standard Created : 6/12/ :30 AM Modified : 2/18/ :07 AM Column : Detection : UV 254nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : 600 L:\ int Unit 4 UV Voltage Time [min.]
12 2/18/ :08 AM Chromatogram L:\int42302.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 4 UV) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
13 2/18/ :09 AM Chromatogram L:\int42299.PRM Page 1 of 2 Chromatogram Info: MC-2302 N6-Benzyladenine, [benzyl ring-14c]- Lot A M W File Name : L:\int42299 File Created : 2/18/ :55:29 AM Origin : Acquired, Acquisition started 3/11/2010 3:09:59 PM Acquired Date : 3/11/2010 3:49:58 PM Project : Test By : Administrator Method : Unit4-40minrun By : Administrator Description : UV trace of blank injection Created : 6/12/ :30 AM Modified : 2/18/ :59 AM Column : Detection : UV 254nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : 8 L:\ int Unit 4 UV 6 4 Voltage Time [min.]
14 2/18/ :09 AM Chromatogram L:\int42299.PRM Page 2 of 2 Result Table (Uncal - L:\int Unit 4 UV) Compound Name Reten. Time [min] [mv.s] No peak to report Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution
15 C:\Xcalibur\data\MC MW 3/8/2010 4:31:41 PM MC MW RT: Relative Abundance Time (min) NL: 2.56E8 TIC F: MS MC MW MC MW #1-199 RT: AV: 199 NL: 8.00E7 T: + c Q3MS [ ] MC MW#1-199 RT: AV: T: + c Q3MS [ ] m/z= m/z Intensity Relative Relative Abundance m/z
16 NAME MC MW EXPNO 1 PROCNO 1 Date_ Time INSTRUM spect PROBHD 5 mm PABBO BB PULPROG zg30 TD SOLVENT MeOD NS 1000 DS 2 SWH Hz FIDRES Hz AQ sec RG 4 DW usec DE 6.50 usec TE K D sec TD0 1 ======== CHANNEL f1 ======== NUC1 1H P usec PL db PL1W W SFO MHz SI SF MHz WDW no SSB 0 LB 0.00 Hz GB 0 PC 1.00 MC2302 1H NMR in MeOD Batch MW ppm
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