Cliquid and MPX Driver Software. Byron Kieser Director Small Molecule System Solutions

Transcription

1 Cliquid and MPX Driver Software Byron Kieser Director Small Molecule System Solutions

2 Cliquid Software 3.0 Improve your bottom line by reducing costs, increasing productivity and data quality using Cliquid Software Reduce your training burden Vastly simplified workflow for technicians Automated reporting Better control of method deployment Cliquid Software We ve made routine LC/MS/MS really, really easy! 2 ASMS Applied Biosystems Inc. and MDS Inc. Joint Owners ABOVE & BEYOND

3 The Cliquid MPX High Throughput System Brings the Ease-of-Use provided by Cliquid Software to Multiplexed LC/MS The MPX Driver Software works seamlessly with Analyst and Cliquid Software to make method development simple and fast The easiest way to create 2-stream multiplexed methods Completely integrated, single data system With the ease-of-use of Cliquid Software and the compliance features of Analyst Software A more cohesive solution to multiplexing for the regulated LC/MS/MS market! 3 ASMS Applied Biosystems Inc. and MDS Inc. Joint Owners ABOVE & BEYOND

4 Come for a demo at ASMS! Cliquid 3.0 Software Let us show you how easy routine LC/MS/MS can be Cliquid MPX -2 High Throughput System MPX Driver Software To arrange a demo, speak to your Applied Biosystems Sales Rep or visit us in the Hospitality/demo suite (Marriott) or the exhibition booth (#106) Some examples of the MPX system development in use are presented this week: Poster WPJ-270: Increased throughput of Vitamin D analysis using a multiple parallel LC-MS system Poster THPZ1-670: High Throughput Quantitative Sample Analysis Using an Integrated Multiplex LC-MS System Combined with On-Line SPE 4 ASMS Applied Biosystems Inc. and MDS Inc. Joint Owners ABOVE & BEYOND

5 Chad Briscoe Director of Bioanalysis MDS Pharma Services, Lincoln, NB High Throughput Multiplexing Strategies using Cliquid MPX -2 Software and Fast Chromatography ThOB-9:10am: Combining high speed chromatography and multistream HPLC systems to increase productivity and efficiency in the bioanalytical laboratory 5 ASMS Applied Biosystems Inc. and MDS Inc. Joint Owners ABOVE & BEYOND

6 Easy to use Multiplexed LC systems for Bioanalysis Laboratories Chad Briscoe, Director of Bioanalysis MDS Pharma Services, Lincoln, NE Sunday May 31 st,

7 Why Multiplexed LC Systems? 7

8 8 More Samples

9 Less Time sample runs are overlapped autosampler starts injection before previous sample is finished 9 higher pressure chromatogarphy

10 Less Money less equipment (only 1 mass spec) one person can run the equivalent of two systems or twice the samples on the same number of systems 10

11 XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) Max. 8.1e4 cps e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. Multiplex LC Designed to Support Two Parallel LC Streams into a Single MS inject idle acquire from stream 1 acquire from stream 2 wait for sync signal to stream N send sync signal Minimizes MS idle time during LC column equilibration Improves LC/MS throughput

12 Shimadzu Hardware Configuration Column switching Valve Degasser 1 Pump B1 Pump A1 + CBM lite Rack Changer 1 Autosampler 1 Loading Pump + CBM lite Degasser 2 Pump B Pump A2 + CBM lite 2. Rack Changer 2 Autosampler 2 Column Oven

13 Shimadzu CTC Hardware Configuration Column switching Valve Degasser 1 Pump B1 Pump A1 + CBM lite Loading Pump + CBM lite Degasser 2 Pump B Pump A2 + CBM lite 2. Column Oven

14 Software NEW Version Cliquid 3.0 Software Analyst 1.5 Software + MPX Driver 14 MPX Driver Software works with Analyst Software or the whole system can be controlled using Cliquid Software

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18 Using the MPX Driver Software with Analyst Software MPX Driver Analyst 1.5 SW 18

19 MPX Methods Screen Superimposed example chromatogram Increased throughput Meter Build gradient profile 19

20 MPX Software Status Screen Stream 1 Acquiring Stream 2 Running Dynamic Flow Diagram 20

21 MPX Software Status Screen Stream 1 Idle Stream 2 Acquiring Dynamic Flow Diagram 21

22 Help is available 22

23 Combining High Speed Chromatography with Multiplexed LC Systems Oral Session Thursday Morning 23

24 Sample preparation and Analysis Sample Prep o Caliper SciClone 96-well Automated Extraction o MTB Ether Liquid-Liquid Extraction Instrument Conditions non-mpx o Isocratic, Acquity UPLC BEH, C18 50x2.1 o 32% acetic acid (0.2%) in MeOH, 0.7 ml/min Throughput o Samples pre-alliquotted with Tecan o 24 plates (2304 samples) per day for 2 staff (8 hours) o Instrument 120 seconds inject to inject o 30 samples/hour = 720 samples / 24 hours >3 instruments needed to keep up with extraction! 24

25 UPLC Isocratic Chromatography Inject to Inject Time ~120s Peak Width at Baseline: ~11s 25 Assay performance excellent with 100% ISR acceptance for both Atorvastatin parent and Ortho metab and 97% for Para metab

26 New Chromatographic Method Generic Method* o Fused Core Supelco Acentis Express 2.1 x 30 mm, 2.7 µ o Column temperature: 40 C o Flow rate: 1.0 ml / min. o Injection volume: 5 µl o Mobile Phase A 0.1% Acetic acid in water o Mobile Phase B 0.1 % Acetic acid in Methanol o Needle Wash 100% Methanol o Pressure Profile 4300 to 5100 psi o Gradient Profile Time %B *From Badman, E.R., Liang, Z., and Bansal,S., Accelerating High Quality Bioanalytical LC/MS Assays in a Regulated Environment. Proceedings of 56th Amer. Soc. Mass Spectrom. Annual Conf. on Mass Spectrom. & Allied Topics, Denver, CO, June, 2008.

27 Fused-Core Silica Fused core silica delivers fast chromatographic separations with lower pressures than sub 2µm particle Allows UHPLC performance on standard HPLC equipment 27

28 MS/MS Method Mass Spectrometer: AB Sciex QTRAP 5500 Source Settings Instrument Settings MRM Table Analyte Q1 Q3 CE CXP Atorvastatin p-hydroxy-atorvastatin o-hydroxy-atorvastatin D5-Atorvastatin D5-p-Hydroxy-atorvastatin D5-o-Hydroxy-atorvastatin o Curtain Gas: 40 o Polarity: Positive o CAD Gas: Medium (9) o Declustering Potential: 125 o Nebulizer Gas: 60 o Entrance Potential: 10 o Desolvation Gas: 60 o Dwell Time: 20 ms o Desolvation Temp: 650 C o Pause Time: 3 ms o Cycle Time: 138 ms

29 Non-MPX Gradient Chromatography XIC of +MRM (6 pairs): 559.3/440.2 Da ID: Atorvastatin from Sample 17 (ATA Max. 3.8e5 cps. 4.0e e5 3.0e5 2.5e5 2.0e5 1.5e5 1.0e5 Start of MPX Acquisition 5.0e Time, min 29 Inject to Inject Time: 118s Peak Width at Baseline: ~3.4s

30 MPX Gradient Chromatography XIC of +MRM (6 pairs): 559.3/440.2 Da ID: Atorvastatin from Sample 17 ( ZZ... Max. 2.7e5 cps. 4.5e5 4.0e5 3.5e5 3.0e5 2.5e e5 1.5e5 1.0e5 5.0e Time, min 30 Inject to Inject Time: 63.5s Peak Width at Baseline: ~3.5s

31 Precision and accuracy results 2 validation batches o Plasma extracts o Standard P&A Injected three times on two days Background o No method development with extracts o First attempt with extracts using MPX conditions. o Samples prepped in Nebraska shipped dry to Toronto 31 LLOQ QC A QC B QC C Atorvastatin Mean CV% 14.9% 9.2% 4.5% 4.6% % Nominal 106% 105% 99% 98% Ortho-hydroxy Mean CV% 23.9% 12.7% 5.5% 6.9% % Nominal 100% 101% 99% 98% Para-hydroxy Mean CV% 16.1% 7.0% 4.4% 2.8% % Nominal 97% 98% 99% 99%

32 21 CFR part 11 Gap Analysis To identify any issues that would prevent use of MPX software in labs operating in a compliant environment MDS PS evaluation has 33 requirements, 4 were raised as concerns 32 o Security (lock-out) -After a predefined number of minutes of inactivity, the application locks, requiring the username and password of the user currently logged in to unlock it. o Data Tags / Flags -The system shall support data tags (e.g., different color, different font, and flags) to indicate which data have been changed or deleted, as documented in the audit trail. o Electronic signatures -Electronic signatures and hand-written signatures executed to electronic records shall be linked to their respective electronic records to ensure that the signature cannot be excised, copied or transferred to falsify an electronic record by ordinary means. o Electronic signatures -Electronic signature shall never be reused or reassigned to anyone else.

33 Benefits Increased Throughput (~2X) o Non-MPX Fused-Core Gradient Method 118 s / sample o MPX Fused-Core Gradient Method 63.5 s / sample Improved Assay Robustness and Efficiency o Injection, column wash off and re-equilibration complete off-line Gradient Methods o Sharper peaks wrt isocratic methods, potential decrease in LOQ o Increased chromatographic peak capacity, reduces interferences from co-eluding compounds. 33

34 Findings Goal of significantly increasing throughput with little additional expense or effort in validation or training was achieved. Expanding lab capabilities to include mutliplexing would require minimal staff training. Compliance with regulations and SOPs can be maintained for multiplexing methods. System is easy to use with intuitive new software and familiar Analyst platform. Can use most existing assays with existing equipment. 34

35 Acknowledgements MDS Pharma Services o Chris Kafonek o Merry Danley o Lee Winchester o Brandon Lester MDS Analytical Technologies Development o Dave Cox o Min Yang o Christine Lai Applications o John Gibbons o Adrian Taylor The trademarks mentioned herein are the property of Applied Biosystems/MDS Analytical Technologies (a joint venture between Applied Biosystems and MDS Inc.) or their respective owners 35

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