Supporting Information
|
|
|
- Marlene Gray
- 5 years ago
- Views:
Transcription
1 Supporting Information Quantitative Profiling of Oxidized Phospholipids in Lipoproteins from Patients with Coronary Artery Disease by Hollow Fiber Flow Field-Flow Fractionation and Nanoflow Liquid Chromatography-Tandem Mass Spectrometry Ju Yong Lee, Seul Kee Byeon, Myeong Hee Moon* Department of Chemistry Yonsei University Seoul, South Korea Table of Contents Operation of MxHF5. S-2 Experimental Conditions of nlc-esi-ms/ms S-3 Figure S1 S-4 Figure S2 S-5 Figure S3 S-6 Table S1 S-7 S-1
2 Operation of MxHF5 Operation of MxHF5 for the semi-preparative scale separation of HDL/LDL/VLDL from plasma samples began by injecting plasma into the injector with the dotted flow paths (injection followed by focusing/relaxation) in Figure 1. Sample components were delivered to the MxHF5 module with 1/10 of the pump flow, which was adjusted by the pressure loop attached to the 4-way valve. During this period, 9/10 of the pump flow was delivered to the outlet end of the MxHF5 so that the two converging flow streams could be focused at a position 1/10 th of each fiber length, while all flow streams exited through the fiber wall as a radial flow so as to provide sample relaxation. After a focusing/relaxation period that is dependent on the radial flow rate, both 3-way and 4-way valves were converted to solid line configuration so that the pump flow was directed to the channel inlet only for the beginning of separation. The second 3-way valve shown in the center of Figure 1 was employed to change the channel outflow rate from & into V & right after the elution of LDL so out, 2 nd Vout, 1 st that long-retaining VLDL could be eluted at an increased outflow rate. Flow rate control for each outlet of the 3-way valve was performed by varying the length of the capillary tube. S-2
3 Experimental Conditions of nlc-esi-ms/ms Figure S1. System configuration of nanoflowlc-esi-ms-ms used for profiling phospholipids, including the lyso- and oxidized forms. For preparation of the capillary column, one end of the empty capillary was sharply pulled out with a flame. The other end of the capillary was immersed to a slurry made by mixing MeOH with C18 beads, and the mixture was used to pack the empty capillary using high pressure N 2 gas. For the analytical column, a Pt wire to support electric power for ESI, pump flow, and the on-off valve was connected to the PEEK microcross as shown in Figure S1. During sample loading from the autosampler to the column, the pump flow with the mobile phase A (10:90 (v/v) CH 3 CN:H 2 O) was delivered at 600 nl/min for 10 min with the on-off valve closed. After loading, the pump flow was raised to 10 µl/min in order to reduce dwell time, and the on-off valve was simultaneously turned on so that the high speed pump flow could be split at the microcross to deliver only 300 nl/min into the analytical column. In order to prevent packing materials in the analytical column from spilling backward during the change of the on-off valve, a high pressure in-line filter unit containing a PEEK frit from IDEX Health & S-3
4 Science Co. was positioned between the microcross and the C18 capillary column as shown in Figure S1. During LC run, a binary gradient separation was applied by increasing the composition of mobile phase B (20:20:60 (v/v/v) CH 3 CN:CH 3 OH:isopropanol). Ionization modifiers were added to both mobile phases: 0.05% ammonium hydroxide in the negative ion mode of the LC run and 0.1% formic acid in the positive ion mode. Gradient elution conditions for the LC run in the negative ion mode of MS were as follows: the mobile phase B was increased to 60% in 1 min, to 80% in 21 min, to 100% in 1 min, and then maintained at 100% for 40 min for the LC run at negative ion mode. In positive ion mode, the mobile phase B was ramped to 80% in 1 min, to 100% in 1 min, and then maintained at 100% over 40 min. Injection amounts of extracted lipids from the lipoprotein fraction were 5 µg in the negative ion mode and 10 µg in the positive ion mode. Run conditions for MS operation during nlc-esi-ms/ms were as follows: ESI voltages were 2.0 kv in the negative ion mode (-) and 3.0 kv in the positive ion mode (+), the mass ranges for the precursor scan were amu (-) and amu (+), the normalized collision energies were 40% (-) and 45% (+), and the isolation widths for CID were 1.5 (-) and 1.0 (+). S-4
5 Figure S2. Base peak chromatograms of the PL standards (2 pmol each) at a) negative mode and b) positive mode by nlc-esi-ms/ms, which are the same run conditions for the plasma lipid extracts from each lipoprotein fraction obtained by MxHF a. negative ion mode MS Intensity 2b 1b 2a 1a :0-LPA 2. 14:0-LPG 3. 12:0/12:0-PA 4. 18:0-LPG 5. 12:0/12:0-PG 6. 16:0/16:0-PA 7. 16:0/18:2-PI 8. 18:0/18:0-PG % of solvent B MS Intensity Time (min.) 3 4 b. positive ion mode :0-LPC 2. 14:0-LPE 3. 12:0-PC 4. 14:0-PE 5. 14:0/16:0-PC 6. 18:0/22:6-PE % of Solvent B Time (min.) S-5
6 Figure S3. BPCs of lipid extracts from each lipoprotein fraction from the pooled CAD patient plasma sample obtained at a) negative ion mode and b) positive ion mode by nlc-esi-ms/ms. a. negative ion mode b. positive ion mode HDL HDL MS Intensity LDL MS Intensity LDL VLDL VLDL Time (min) Time (min.) S-6
7 Table S1. Identified PLs and Ox-PLs from each lipoprotein class besides PI class. Each species detected in pooled control (C) and patients (P) sample is checked with O. PA C/P C/P C/P C/P C/P C/P :0 16:0 o/o o/o o/o lyso 18:0 o/o o/o o/o :0 16:1 o/o :0 lyso o/ /o /o :0 18:1 o/o o/o o/o :1 lyso o/o o/o /o :0 18:2 o/o o/o o/o lyso 18:2 o/ o/o o/o :0 20:0 o/o /o o/o :2 lyso /o :0 20:3 /o o/o o/o lyso 18:3 /o :0 20:4 o/o o/o o/o :3 lyso /o /o :0 20:5 o/o /o /o :0 lyso o/ :0 22:1 o/o o/ :1 lyso /o :0 22:2 o/o o/o :2 lyso o/o :0 22:3 o/ lyso 20:3 /o :0 22:5 o/ o/o o/ :3 lyso /o :0 22:6 o/o o/o o/o :4 lyso o/o o/o o/o :0 24:3 o/ o/ :5 lyso /o :0 24:4 o/o o/ o/o :6 lyso o/ o/ o/o :1 18:1 o/ :0 10:3CHO o/o /o :1 18:2 o/ o/ /o :0 10:3COOH+O /o :0 18:0 o/o o/o o/o :0 11:0CHO /o :0 18:1 o/ o/ o/ :0 11:2CHO /o :0 18:2 o/o o/o o/o :0 18:1+O /o :0 20:0 o/ o/ o/o :0 18:2+O o/o o/o o/o :0 20:3 o/ o/ :0 20:3+2O o/o o/ :0 20:4 o/o /o :0 20:3+OO /o /o :0 22:1 o/ :0 20:4+2O o/o o/ :0 22:2 o/ o/o :0 20:4+O /o o/o o/o :0 22:3 o/ :0 20:5+2O /o :0 22:5 o/o /o o/o :0 20:5+O o/o /o :0 22:6 o/o o/o o/o :0 22:5+2O o/ o/ :1 18:1 o/ /o o/o :0 22:5+OO /o :1 20:2 /o :0 22:5+O o/o :1 22:6 o/o o/o o/o :0 22:6+2O o/ o/ o/ :2 18:1 o/o o/ :0 22:6+O o/ o/o o/o :2 18:2 o/o o/o :0 6:5CHO o/ :2 22:6 o/ :0 10:3CHO o/o /o :1 20:4 o/o o/ :0 18:2+O o/o o/o o/o S-7
8 :1 22:6 /o o/o :0 20:3+2O o/ :4 18:1 /o :0 20:4+2O /o /o :4 20:0 o/o /o o/ :0 20:4+O o/o o/o o/o :5 18:0 o/o o/o /o :0 21:5CHO o/ :5 20:1 o/ :0 22:5+O /o /o :4 18:0 o/ :0 22:6+O o/ /o /o :5 20:0 o/ :0 6:0COOH /o :5 20:1 o/ :1 10:3CHO o/o o/o o/o :6 20:0 o/o o/ :1 18:2+O o/o o/o o/o lyso 14:0 o/ o/o /o :2 10:3CHO /o o/ :0 lyso o/o o/o o/o :2 18:2+O o/o lyso 16:0 /o :2 7:1CHO o/ :0 lyso o/o o/o o/o :2+O 18:1 o/ lyso 16:1 o/ :2+O 18:2 o/o :3 lyso o/ /o /o PG C/P C/P C/P C/P C/P C/P :0 16:0 o/ o/o :2 16:0 /o :0 20:1 o/ :6 16:0 o/o /o :1 16:0 /o :0 lyso o/o o/o :0 16:0 /o o/o o/ :0 lyso /o o/o o/o :0 18:0 /o o/o lyso 16:3 o/ :1 16:0 o/o o/o o/o lyso 18:0 o/o o/o :1 16:1 o/o o/ :0 lyso o/o /o o/o :1 18:0 o/ :1 lyso /o o/o :1 18:1 /o o/o /o :2 lyso /o :1 18:2 o/o o/o o/o :5 lyso /o :2 16:0 o/o o/o o/o :2+O 18:0 o/ /o :2 16:1 o/o o/o :2+OO 18:0 /o :2 18:0 o/o /o :2+O 16:0 o/o o/o o/o :2 18:2 o/ o/ o/ :3+O 16:0 o/o /o :3 16:0 o/o o/o /o :3+OO 16:0 /o /o :1 16:1 o/o o/ o/ :6+O 16:0 /o :1 18:3 o/ :6+2O 16:0 /o PE S-8
9 C/P C/P C/P C/P C/P C/P :0 18:2 /o /o :0 lyso o/o o/o /o :0 20:5 o/o o/ :1 lyso /o o/ /o :1 20:4 o/o o/o :0 lyso /o o/o /o :1 18:1 /o o/o lyso 18:1 /o o/ /o :1 18:2 o/o o/o o/o :1 lyso /o o/o /o :1 20:3 o/o o/o o/ :2 lyso o/o :2 20:0 /o o/ :3 lyso o/o o/o :1 20:4 o/ :4 lyso o/o o/o :6 18:0 o/ o/o /o lyso 20:6 /o /o :6 18:1 /o :6 lyso o/o /o :0 16:0 o/o /o :1 18:2+O o/o o/o :1 16:0 o/o /o :0 20:5+O o/o :0 22:6 o/o o/o :6 18:0+O /o o/o /o :6 18:0 o/o o/o o/o :6 18:0+OO /o /o :0 16:0 o/ PC C/P C/P C/P C/P C/P C/P :0 18:0 /o lyso 16:0 /o /o :0 18:1 o/ o/o :0 lyso /o o/o /o :0 14:0 o/ :1 lyso /o o/o /o :0 16:0 o/o o/o o/o lyso 18:0 o/ o/ :0 16:1 o/ /o :0 lyso o/o o/ o/ :0 18:1 o/o o/o o/o lyso 18:1 /o /o o/o :0 18:2 o/o o/o o/o :1 lyso o/o o/o o/o :0 18:3 o/ o/o o/ lyso 18:2 /o o/o o/o :0 20:2 o/o o/o :2 lyso o/o o/o o/o :0 20:3 o/o o/o /o lyso 18:3 o/ o/o o/o :0 20:4 o/o o/ :3 lyso o/o /o :0 20:5 o/o o/o o/o :1 lyso /o o/o :0 22:3 o/ lyso 20:2 o/o :0 22:5 /o :2 lyso o/o o/o /o :1 16:1 o/ /o o/ lyso 20:3 o/ :1 18:1 o/o o/o o/o :3 lyso /o o/o :1 18:2 o/ o/ /o :4 lyso o/o o/o /o :1 22:5 o/o /o lyso 20:5 o/ /o :0 16:0 o/o o/o o/ :5 lyso /o o/o /o :0 18:0 o/o o/o lyso 20:6 o/ S-9
10 :0 18:1 o/o o/o o/o :6 lyso o/ o/o :0 18:2 o/o o/o /o :3 lyso o/ :0 18:3 o/o o/o /o :4 lyso o/ :0 20:3 o/o o/o o/o :5 lyso o/o /o :0 20:4 o/o o/o o/o :6 lyso o/o o/o :0 20:5 o/o o/o o/o :0 8:2CHO /o :0 22:4 o/o o/o :0CHO 16:0 o/ :0 22:6 o/o o/o o/o :0 9:0CHO o/o /o o/ :1 14:0 o/ :0 10:1CHO o/o /o /o :1 18:1 o/o o/ o/o :0 8:0CHO /o :1 18:2 o/o o/o o/o :0 8:1CHO+OO /o :1 20:1 o/o :0 9:2CHO o/o :1 20:4 o/o o/ /o :0 11:1CHO o/o o/o :2 14:0 o/o :0 9:0CHO /o /o o/ :2 18:2 /o :0COOH 16:0 o/ :2 18:3 o/ o/ :3CHO 16:0 /o /o :2 22:0 o/ :1CHO 16:0 o/ :2 22:6 o/ /o :0 10:1CHO /o :3 14:0 o/ /o o/ :0CHO 16:0 o/o :3 16:0 o/o o/o o/o :0 9:0COOH /o /o :3 16:1 o/ o/ o/ :3CHO 18:0 /o :3 18:1 o/ /o /o :0 11:1CHO /o :3 18:2 o/o :0COOH 16:0 /o :4 16:0 o/ :4CHO 16:0 /o /o :0 18:2 o/ :0 14:3CHO o/ :1 16:0 /o /o /o :0 12:3CHO o/o :2 18:0 o/ o/o :0 12:2CHO /o /o o/o :4 14:0 o/o o/o /o :0CHO 16:0 o/o :4 16:1 o/ o/ :5CHO 16:0 /o /o :5 14:0 o/o o/ :2 16:1CHO o/ /o :5 16:1 /o /o :0 18:3+O o/ o/o o/ :5 18:2 /o o/ :1+O 18:2 o/o /o :6 16:0 o/o o/o o/o :0 18:2+O o/o o/o /o :6 18:0 o/ /o :0 18:1+O /o o/o :0 16:0 o/ :0 17:0COOH /o /o :1 18:2 o/ /o :0 20:5+O o/o /o :2 16:0 o/ :0 18:3+O o/o o/o /o :4 16:0 /o o/o o/ :1+O 16:0 /o /o /o :5 18:0 o/o /o /o :0 18:2+O&OO o/ :5 18:1 o/ /o :0 20:5+OO /o /o o/ :6 14:0 /o /o :1+OO 16:0 /o /o S-10
11 :6 16:0 o/o o/o o/o :0 20:5+O /o :6 16:1 o/ o/o :4+O 16:0 /o o/o :6 18:1 o/o o/ o/o :0 18:3+OO&O /o :5 18:0 o/ o/o o/ :5+O 18:0 o/o /o :0 lyso /o /o S-11
Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS
Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Agilent Technologies Notices Agilent Technologies, Inc. 2008 No part of this manual may be reproduced in any form or by any
Q Exactive HF-X QuickStart Guide
Q Exactive HF-X QuickStart Guide About this Guide Q Exactive HF-X Tune This Q Exactive HF-X QuickStart Guide gives an introduction on setting up and using the Thermo Scientific Q Exactive HF-X mass spectrometer.
Agilent GC/MSD Instructions
Agilent GC/MSD Instructions GC/MS SAMPLE PREPARATION: Sample Components to Avoid Completely: The following should never be injected: metals, strong acids or bases, salts, oligomeric and polymeric material.
Using the Chromeleon Summit 2 Tandem Operation Wizard
Technical Note 702 Using the Chromeleon Summit 2 Tandem Operation Wizard 1 INTRODUCTION New Summit 2 Tandem Operation support of the Program Wizard is available in Chromeleon version 6.70. The purpose
Bio Compatibility Kit
General Information 1 Installing the 2 How to Use the 5 Important Safety and Solvent Compatibility Considerations 5 Setting the Minimum or Maximum Operating Pressure in ChemStation 7 Setting the Minimum
Statistical Process Control in Proteomics SProCoP
Statistical Process Control in Proteomics SProCoP This tutorial will guide you through the installation of SProCoP and using it to perform statistical analysis on a sample Skyline file. Getting Started
HPLC Basics, Equipment, and Troubleshooting
HPLC Basics, Equipment, and Troubleshooting HPLC Basics, Equipment, and Troubleshooting explains chromatography in practical terms from the ground up COURSE HANDOUT: [SECTION 14] Troubleshooting & Diagnostics
Steps for LCMS-8040 Triple Quadrupole
Steps for LCMS-8040 Triple Quadrupole June 2017 Version 2 A. Data Acquisition 1. Check lights on MS. There should be a total of three lights lit by LED. One of them is blinking. Power Blinking Gas Status
Quick Setup Instructions for the Knauer HPLC-System. Quick Setup Instructions for the Knauer HPLC-System For windows XP (using CHANCE vers. 2.
For windows XP (using CHANCE vers. 2.0) Compiled by P. Madl Vers. 1.0 June 2011 1 / 9 1. Hardware Setup: Result 1.1. Attach RS 232 interface cable (designated as PC) to COM-port-1 of the laptop. 1.2. connect
Agilent MassHunter Workstation Software 7200 Accurate-Mass Quadrupole Time of Flight GC/MS
Agilent MassHunter Workstation Software 7200 Accurate-Mass Quadrupole Time of Flight GC/MS Familiarization Guide Before you begin 3 Prepare your system 3 Prepare the samples required for data acquisition
Thermo. EASY-nLC User Guide for LC Devices
Thermo EASY-nLC 1200 User Guide for LC Devices 60053-97273 Revision A September 2015 2015 Thermo Fisher Scientific Inc. All rights reserved. AFC, EASY-nLC, EASY-Column, EASY-Spray, Foundation, LC TriPlus,
Agilent G1978A Multimode Source for 6300 Series Ion Trap LC/MS
Agilent G1978A Multimode Source for 6300 Series Ion Trap LC/MS Set-Up Guide Agilent Technologies Notices Agilent Technologies, Inc. 2008 No part of this manual may be reproduced in any form or by any means
The Agilent 5975 inert MSD: New Tools for the Forensic Analyst
The Agilent 5975 inert MSD: New Tools for the Forensic Analyst Chemical Analysis Group Bruce D. Quimby, Ph.D. Senior Applications Chemist Agilent Technologies Wilmington, Delaware March 2,2006 Page 1 The
Xcalibur. QuickQuan. User Guide. XCALI Revision C July For Research Use Only Not for use in Diagnostic Procedures
Xcalibur QuickQuan User Guide XCALI-97195 Revision C July 2007 For Research Use Only Not for use in Diagnostic Procedures 2007 Thermo Fisher Scientific Inc. All rights reserved. Microsoft, Access, Excel,
Agilent 1100 HPLC ChemStation 1) Turn on all the modules for HPLC system (CapPump G1376A, DAD G1315B) and allow for system to initialize.
Standard Operating Procedure Remcho Research Group P a g e 1 Agilent 1100 HPLC ChemStation 1) Turn on all the modules for HPLC system (CapPump G1376A, DAD G1315B) and allow for system to initialize. 2)
EASY-nLC Series User Guide for the Xcalibur Data System (Xcalibur version 2.1 or later)
Thermo EASY-nLC Series User Guide for the Xcalibur Data System (Xcalibur version 2.1 or later) P/N 60053-97230 Revision B January 2013 2013 Thermo Fisher Scientific Inc. All rights reserved. EASY-nLC,
Retention time prediction with irt for scheduled MRM and SWATH MS
Retention time prediction with irt for scheduled MRM and SWATH MS EuPa course, 28.11.2013 Oliver Rinner, PhD CEO Biognosys Summary Why to schedule MRM acquisition? How to schedule? Prediction of retention
Agilent 6100 Series Quad LC/MS System
Agilent 6100 Series Quad LC/MS System Quick Start Guide Where to find more information 2 Configuration Diagrams 3 ChemStation Views 5 Basic Operation 20 Operating Tips 27 Troubleshooting Tips 29 Safety
Installation Instructions
Analyst QS Service Pack 5 (SP5) Release Notes Final Version Analyst QS Service Pack 5 includes all previous improvements released in Analyst QS SP1 through Analyst QS SP4. It addresses a number of issues
Sepacore X10 / X50 system Technical data sheet
Sepacore X10 / X50 system Technical data sheet Sepacore X10 and X50 flash chromatography systems address most requirements for the purification of organic compounds. Whether a crude synthesis mixture or
Configuring and optimizing an IC-MS system using a compact IC and a single quadrupole mass spectrometer
TECHNICAL NOTE 72611 Configuring and optimizing an IC-MS system using a compact IC and a single quadrupole mass spectrometer Authors Terri Christison and Jeff Rohrer Thermo Fisher Scientific Sunnyvale,
Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS
Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS Set-Up Guide Agilent Technologies Notices Agilent Technologies, Inc. 2008 No part of this manual may be reproduced in any form or by any means
Agilent G6855AA MassHunter Personal
Agilent G6855AA MassHunter Personal Forensic Toxicology Database Kit Quick Start Guide What is the MassHunter Personal Forensic Toxicology Database Kit? 1 Kit Content 2 Where to find more information 4
Operating Manual. High Performance Liquid Chromatograph. Scientific Equipment Center, Prince Of Songkla University
Operating Manual High Performance Liquid Chromatograph Agilent 1100 ; VWD, DAD, FLD and RID Scientific Equipment Center, Prince Of Songkla University Operating Manual High Performance Liquid Chromatograph
Optimizing System Dispersion on the Agilent 1290 Infinity II LC
Optimizing System Dispersion on the Agilent 1290 Infinity II LC The Agilent Ultralow Dispersion Kit Technical Overview Authors Bettina Schuhn and Sonja Schneider Agilent Technologies, Inc. Waldbronn, Germany
Cliquid and MPX Driver Software. Byron Kieser Director Small Molecule System Solutions
Cliquid and MPX Driver Software Byron Kieser Director Small Molecule System Solutions Cliquid Software 3.0 Improve your bottom line by reducing costs, increasing productivity and data quality using Cliquid
MassHunter Pesticides PCD or PCDL Quick Start Guide
MassHunter Pesticides PCD or PCDL Quick Start Guide What is the MassHunter Pesticides PCD or PCDL? 3 Kit Contents 4 Where to find more information Before You Begin 7 Installation 7 Required reagents and
ChromQuest 5.0 Quick Reference Guide
ChromQuest 5.0 Quick Reference Guide This guide contains an overview of the ChromQuest chromatography data system, with topics organized by workflow. For more information, refer to the ChromQuest User
Guard Columns ---- HPLC Column Catalog
Guard ---- Guard s selection and Use 092 Cartridge Guard E 093 Cartridge Guard Ei (Non-metal type) 094 Guard for UHPLC 096 GL Cart 098 Packed Guard, Mini Guard 099 SILFILTER TM STD C18 4 Filters, Impurity
Tutorial: Modeling Liquid Reactions in CIJR Using the Eulerian PDF transport (DQMOM-IEM) Model
Tutorial: Modeling Liquid Reactions in CIJR Using the Eulerian PDF transport (DQMOM-IEM) Model Introduction The purpose of this tutorial is to demonstrate setup and solution procedure of liquid chemical
Technical Overview. Introduction
Performance characteristics of the Agilent 12 Infinity Series Variable Wavelength Detectors Faster results, improved sensitivity and absolute data security Technical Overview Introduction The Agilent 12
Manual HPLC-4 Alliance-UV
Manual HPLC-4 Alliance-UV MAKE SURE NEVER TO: 1. pump particles or air through the column. 2. inject when the injector is dry. 3. inject solutions containing particles. 4. apply large (sudden) pressure
Release Note for Agilent LC and CE Drivers Revision A.02.16
Release Note for Agilent LC and CE Drivers Revision A.02.16 Introduction This release note provides important information for the release of Agilent LC and CE drivers A.02.16. The Agilent LC and CE Driver
Agilent G6854AA MassHunter Personal Pesticide Database Kit Quick Start Guide
Agilent G6854AA MassHunter Personal Pesticide Database Kit Quick Start Guide What is the MassHunter Personal Pesticide Database Kit? 1 Kit Content 2 Where to find more information 3 Before You Begin 4
UniPoint System Software User s Guide
UniPoint System Software User s Guide LT2137 1998 Gilson, Inc. All rights reserved Exercise 3-Creating operations list... 4-22 Create and name operations list... 4-22 Set up steps... 4-23 Identify sample
Agilent Splitter Kits
Agilent Splitter Kits Installation and Configuration This technical note describes the installation and configuration of the Agilent Splitter Kits in an Agilent 1260 Infinity Series System or an Agilent
Samples Preparing the GC to run samples Running samples - Manual Injection Running samples - GC ALS or Valve Injection
27 Samples Preparing the GC to run samples Running samples - Manual Injection Running samples - GC ALS or Valve Injection Methods Creating Methods Setting a Column Flow Rate or Pressure Sequences Creating
Agilent 6300 Ion Trap LC/MS Systems Quick Start Guide
Agilent 6300 Ion Trap LC/MS Systems Quick Start Guide A guide to help you get started with the Agilent 6300 Series Ion Trap LC/MS system What is the Agilent 6300 Series Ion Trap LC/MS System? 2 Where to
Montgomery County Community College Document Number: DP DeKalb Pike Revision Number: 2. Page 1 of 9 SOP: BioLogic LP Chromatography System
Page 1 of 9 Approvals Preparer: Jason McMillan Reviewer: Dr. Maggie Bryans Date: 18DEC14 Date: 18DEC14 1. Purpose: Operation of the BioLogic LP Chromatography System. 2. Scope: Applies to the BioLogic
Hardware Guide. AB SCIEX QTRAP 5500 System
Hardware Guide AB SCIEX QTRAP 5500 System D1000079384 E May 2010 This document is provided to customers who have purchased AB SCIEX equipment to use in the operation of such AB SCIEX equipment. This document
Release Note for Agilent LC and CE Drivers Revision A.02.15
Release Note for Agilent LC and CE Drivers Revision A.02.15 Introduction This release note provides important information for the release of Agilent LC and CE drivers A.02.15. The Agilent LC and CE Driver
Isolera. User Manual
Isolera User Manual Safety and Document Conventions Isolera system should only be operated and maintained by trained individuals. Please read this manual carefully before working with the system. To guarantee
User Manual. Agilent 1260 Infinity HPLC-Chip Cube Interface (G4240A )
User Manual Agilent 1260 Infinity HPLC-Chip Cube Interface (G4240A ) Notices Agilent Technologies, Inc. 2010, 2012 No part of this manual may be reproduced in any form or by any means (including electronic
Agilent 1290 Infinity 2D-LC Solution
Agilent 1290 Infinity 2D-LC Solution A new flexible and userfriendly 2D-LC solution for the most complex samples Michael Frank Marketing Manager Analytical HPLC LPSD, Waldbronn, Germany April, 13 th 2012
Customers should provide all necessary operating supplies upon request of the engineer.
Agilent Installation Infinity Checklist II Preparative HPLC System Thank you for purchasing an Agilent instrument solution. This checklist is used by the installing engineer to ensure that the instrument
Aqueous GPC Instructions - Detailed
Aqueous GPC Instructions - Detailed Waste Bottle Columns PL Datastream Injection Port GPC Computer UV Detector GPC Pump Eluent Bottle The Aqueous GPC Set Up RI Detector Important The points below must
Andreas Reimann Berlin im Juli 2009
Agilent Technologies ASMS 2009 Andreas Reimann Berlin im Juli 2009 New Triple Quad LC/MS Portfolio 6410, 6430 & 6460 Page 2 Comparison of 6400 Series QQQs 6410BA 6430 6460 Sensitivity: 1 pg reserpine S/N
SepacoreControl Software data sheet
SepacoreControl Software data sheet The software is able to control the Sepacore flash X and X5 chromatography system. Designed and developed with and for laboratories, the software is intuitive, easy
Automated Fraction Re-Analysis Does it really make sense?
Automated Fraction Re-Analysis Does it really make sense? Presented by: Udo Huber 1995 PHD in organic chemistry from the University Karlsruhe/Germany 1996-97 Postdoctoral fellow at the University of Hawai
MS data processing. Filtering and correcting data. W4M Core Team. 22/09/2015 v 1.0.0
MS data processing Filtering and correcting data W4M Core Team 22/09/2015 v 1.0.0 Presentation map 1) Processing the data W4M table format for Galaxy 2) Filters for mass spectrometry extracted data a)
Standard Operating Procedure for the HPLC-ICP-MS System By Celina Dozier Fall 2017
Standard Operating Procedure for the HPLC-ICP-MS System By Celina Dozier Fall 2017 Introduction This document is only for operation of the HPLC and ICP-MS instruments to obtain data. It contains no maintenance
Agilent 6400 Series Triple Quadrupole LC/MS System
Agilent 6400 Series Triple Quadrupole LC/MS System Quick Start Guide Where to find information 4 Getting Started 6 Step 1. Start the Data Acquisition software 7 Step 2. Prepare the LC modules 13 Step 3.
Standard: 2 x 500 ml and 2 x 250 ml (safety) bottles with shut-off valve and 2 µm in-line filter. Built-in 4 channel He degasser.
The UltiMate Solvent Organizer 3.9 Specifications 3.9.1 Solvent Organizer Reservoirs Solvent Degassing Standard: 2 x 500 ml and 2 x 250 ml (safety) bottles with shut-off valve and 2 µm in-line filter.
Porous Reactor with Injection Needle
Porous Reactor with Injection Needle Introduction This model treats the flow field and species distribution in an experimental reactor for studies of heterogeneous catalysis. The model exemplifies the
MassLynx 4.1 SCN 804. Release Notes. SQ Detector 2
R E L E A S E N O T E S MassLynx 4.1 SCN 804 Release Notes SQ Detector 2 43464 Rev 01 Copyright Waters Corporation 2011. All rights reserved. Copyright 2011 Waters Corporation 43464_1. Waters is a registered
CHROMABOND Flash cartridges
Flash chromatography outperforming the standard CHROMABOND Flash cartridges from the silica experts www.mn-net.com MN products for Flash chromatography Contents CHROMABOND Flash solutions separations from
Unprecedented In Water Imidazole- Carbonylation: Paradigm shift for Preparation of Urea and Carbamate
Unprecedented In Water Imidazole- Carbonylation: Paradigm shift for Preparation of Urea and Carbamate Kamlesh J. Padiya* Sandip Gavade, Bhavana Kardile, Manojkumar Tiwari, Swapnil Bajare, Madhav Mane,
Chromeleon / MSQ Plus Operator s Guide Document No Revision 03 October 2009
MSQ Plus Chromeleon / MSQ Plus Operator s Guide Document No. 065322 Revision 03 October 2009 Chromeleon / MSQ Plus Operator s Guide 2009 by Dionex Corporation All rights reserved worldwide. Printed in
Detailed procedures, operation instructions, maintenance, and emergency contact information list is attached.
GC/FID Standard Operating Procedure Environmental health and Safety (EH&S) 2809 Daley Drive Ames, IA 50011-3660 Phone: (515)-294-5359 Fax: (515)-294-9357 www.ehs.iastate.edu Procedure: Department: Bioeconomy
FractionLynx Software
FractionLynx Software User s Guide 34 Maple Street Milford, MA 01757 71500054302, Revision A NOTICE The information in this document is subject to change without notice and should not be construed as a
Accessories for
Accessories for 29420500 Below, the accessories are grouped into Scope of delivery and Optional accessories. Please keep this printout at hand for ordering replacement material. These lists may be subject
BioTOF. Series. Getting Started
BioTOF Series Getting Started Copyright Copyright 2003. Bruker Daltonics Inc. All Rights Reserved. Reproduction, adaptation, or translation without prior written permission is prohibited, except as allowed
MS-FINDER tutorial. Last edited in Aug 10, 2018
MS-FINDER tutorial Last edited in Aug 10, 2018 Abstract The purpose of metabolomics is to perform the comprehensive analysis for small biomolecules of living organisms. Gas chromatography coupled with
Hardware Overview. AB SCIEX TripleTOF 5600 System
Hardware Overview AB SCIEX TripleTOF 5600 System Part Number: 5012938 A January 2011 This document is provided to customers who have purchased AB SCIEX equipment to use in the operation of such AB SCIEX
Huqun Liu, Varian Inc., Lake Forest CA
Rapid development of an LC method for separating high molecular weight degradants from a biopharmaceutical product using an automated Design of Experiments (DOE) approach. Introduction Huqun Liu, Varian
PurityChrom Bio 5 Software Manual V2650
PurityChrom Bio 5 Software Manual V2650 Introduction 3 Table of Contents Note: For your own safety, read the manual and always observe the warnings and safety information on the device and in the manual!
Data processing. Filters and normalisation. Mélanie Pétéra W4M Core Team 31/05/2017 v 1.0.0
Data processing Filters and normalisation Mélanie Pétéra W4M Core Team 31/05/2017 v 1.0.0 Presentation map 1) Processing the data W4M table format for Galaxy 2) A generic tool to filter in Galaxy a) Generic
Operation of the Toxic Organic Chemical Air Monitor (TOCAM ) with the Ellvin Chromatography Software
Operation of the Toxic Organic Chemical Air Monitor (TOCAM ) with the Ellvin Chromatography Software April 13, 2015 Defiant Technologies, Inc. TOCAM(R) 1 Basic Parts of the Toxic Organic Chemical Air Monitor
2-1. the ESI Probe Assembly. the PEEK Safety Sleeve and Sample Tube to the ESI Probe
You tune and calibrate your LCQ Advantage in ESI mode before you acquire data in either the ESI or the APCI mode. This chapter contains the following topics:.removing.connecting.installing.setting.setting
Modeling Evaporating Liquid Spray
Tutorial 16. Modeling Evaporating Liquid Spray Introduction In this tutorial, FLUENT s air-blast atomizer model is used to predict the behavior of an evaporating methanol spray. Initially, the air flow
How to Run the CASPiE GCMS
How to Run the CASPiE GCMS A GCMS (gas chromatograph-mass spectrometer) can be used to separate and identify complex mixtures. You will use the CASPiE GCMS to acquire the mass spectrum of your anitviral
Agilent Max-Light Cartridge Cell Information for G4212A/B DAD and G7117A/B DAD
Agilent Max-Light Cartridge Cell Information for G4212A/B DAD and G7117A/B DAD Information about the Max-Light Cartridge Cells 2 Specifications 2 Description of the dispersion volume V(σ) 2 Recommendations
ANALYTICAL NovoGROM COLUMNS 3.0, 4.0, and 4.6 mm i.d.
ANALYTICAL NovoGROM COLUMNS 3.0, 4.0, and 4.6 mm i.d. The analytical NovoGROM HPLC column system is many-fold proven. These columns can be connected to any HPLC system either conventionally via 1/16" capillary,
ACQUITY UPLC Systems with 2D Technology Capabilities Guide
ACQUITY UPLC Systems with 2D Technology Capabilities Guide Revision A Copyright Waters Corporation 2012 All rights reserved Copyright notice 2012 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA
Modeling Evaporating Liquid Spray
Tutorial 17. Modeling Evaporating Liquid Spray Introduction In this tutorial, the air-blast atomizer model in ANSYS FLUENT is used to predict the behavior of an evaporating methanol spray. Initially, the
Agilent G3250AA LC/MSD TOF System
Agilent G3250AA LC/MSD TOF System Concepts Guide The Big Picture Agilent Technologies Notices Agilent Technologies, Inc. 2003-2007 No part of this manual may be reproduced in any form or by any means (including
HiQ sil TM HPLC Columns
www.kromatek.co.uk KYA Technologies of Japan KYA Technologies Corporation, Tokyo, Japan established in 1998 to develop a range of HPLC products. The latest product HiQ sil HS is a high performance silica
MICRO GC FUSION Micro GC Fusion 3/17/2014 1
MICRO GC FUSION 1 Micro GC Fusion The Next Generation Micro GC 2 Builds on an Innovative Tradition 3000 Micro GC Micro GC Fusion MTI Micro GC 3 Micro GC Fusion Feature Highlight New Chassis Design Aesthetic,
Agilent 1290 Infinity with ISET
Agilent 1290 Infinity with ISET User Manual Agilent Technologies Notices Agilent Technologies, Inc. 2012, 2013 No part of this manual may be reproduced in any form or by any means (including electronic
PROTOCOL: GCP MS Analysis (MSA)
PROTOCOL: GCP MS Analysis (MSA) Purpose Analyze and relatively quantify post translational modifications on core histone H3 with a fully targeted mass spectrometry assay. This protocol should be started
This tutorial illustrates how to set up and solve a problem involving solidification. This tutorial will demonstrate how to do the following:
Tutorial 22. Modeling Solidification Introduction This tutorial illustrates how to set up and solve a problem involving solidification. This tutorial will demonstrate how to do the following: Define a
ABB Analytical ABB Process Analytics. VistaII. Model 2000 Process Gas Chromatograph
ABB Analytical ABB Process Analytics VistaII Model 2000 Process Gas Chromatograph A better gas chromatograph...built on a foundation of leadership ABB Process Analytics manufactured and sold the first
Mass spectrometry assay optimization using functional programming patterns in Lua
Mass spectrometry assay optimization using functional programming patterns in Lua Bennett Kalafut Lua Workshop 2016, San Francisco, CA The world leader in serving science Outline 1. Mass Spectrometry and
GC-MS ChemStation Reference Guide. Last Updated July 2014
GC-MS ChemStation Reference Guide Last Updated July 2014 Overview GC-MS Instrumentation Description Sequence Programming Data Analysis Tips and Tricks Method programming reference is for your enrichment
3 Starting the chiral HPLC and logging into LC Real Time Analysis.
Edition: 1.2 Date: 7-Oct-11 Page 1 of 5 1 Responsibilities. First person responsible: Mariëlle Delville. Second person responsible: Helene I.V. Amatdjais-Groenen. 2 Definitions. Read the information on
Hardware Update. John Jayne 5 th AMS User s Meeting 10/11/04
Hardware Update John Jayne 5 th AMS User s Meeting 10/11/04 Hardware TOF AMS Light Scattering module Short chamber Beam Width Probe High pressure lens for larger size particles Rigid critical aperture
Chromatography Software Training Materials. Contents
Chromatography Software Training Materials This document contains information on how to build a method, start the instrument to acquire data, and then process the data using the Galaxie Program. You will
Waters Quattro micro API Mass Spectrometer Operator s Guide
Waters Quattro micro API Mass Spectrometer Operator s Guide 71500058602/Revision D Copyright Waters Corporation 2003 2010 All rights reserved Copyright notice 2003 2010 WATERS CORPORATION. PRINTED IN THE
OpenLynx User's Guide
OpenLynx User s Guide OpenLynx User's Guide Version 4.0 Waters part No 715000405 Micromass Part No - 6666670 5 February, 2002 i OpenLynx User s Guide OpenLynx User s Guide The software described in this
LTQ Velos SP3 Release Notes
LTQ Velos 2.6.0 SP3 Release Notes These release notes briefly list changes in the LTQ Velos 2.6.0 SP3 experiment setup, tuning, and calibration application; fixes to previously reported issues; and known
Tutorial: Hydrodynamics of Bubble Column Reactors
Tutorial: Introduction The purpose of this tutorial is to provide guidelines and recommendations for solving a gas-liquid bubble column problem using the multiphase mixture model, including advice on solver
Release Note for Agilent LC and CE drivers Revision A.02.14
Release Note for Agilent LC and CE drivers Revision A.02.14 Introduction This release note provides important information for the release of Agilent LC and CE drivers A.02.14. The 1260 Infinity II LC is
To NeSSi or Not to NeSSi: A Fast and Micro GC Experience Review
Falcon Analytical makers of the... To NeSSi or Not to NeSSi: A Fast and Micro GC Experience Review IFPAC Annual Meeting January 26, 2016 Grand H NeSSi Arlington, VA (Washington DC), U.S.A 433 AEI Drive
Agilent OpenLAB CDS. Version 2.2. Release Notes. OpenLAB CDS Release Notes 1
Agilent OpenLAB CDS Version 2.2 Release Notes OpenLAB CDS Release Notes 1 Introduction This document provides a listing of the major feature modifications made in each release of the Agilent OpenLAB Chromatography
Agilent 7890 Series Gas Chromatograph
Agilent 7890 Series Gas Chromatograph GC Software Features Overview Agilent Technologies Notices Agilent Technologies, Inc. 2013 No part of this manual may be reproduced in any form or by any means (including
What s New in Empower 3
What s New in Empower 3 Revision A Copyright Waters Corporation 2010 All rights reserved Copyright notice 2010 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED.
Flow in an Intake Manifold
Tutorial 2. Flow in an Intake Manifold Introduction The purpose of this tutorial is to model turbulent flow in a simple intake manifold geometry. An intake manifold is a system of passages which carry
PRM Method Development and Data Analysis with Skyline. With
Tutorial Webinar #17 PRM Method Development and Data Analysis with Skyline With Brendan MacLean (Principal Developer, Skyline) Eduard Sabidó, Ph.D. (Head of the UPF/CRG Proteomics Unit) Cristina Chiva,
Chromeleon software orientation
Chromeleon software orientation Upon opening of Chromeleon shortcut, a blue screen should appear (called control panel). If this does not occur, the green circled shortcut will open this screen. To ensure
ANALYTICAL SYSTEMS. L3320 Autosampler with excellent accuracy and linearity. System configuration examples
ANALYTICAL SYSTEMS Modular HPLC system can be configured according to customer needs from components as follows: PDA Detector with wavelength range 200-800 nm with scanning possibility or UV-VIS Detector