Agilent 5977 MSD Overview
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1 Agilent 5977 MSD Overview New GC/MSD Introduction January
2 New Agilent 5977 Series GC/MSD The 5977 is the new Agilent GC/MSD platform and replaces the 5975C and 5975E systems Improves performance with high sensitivity Extractor Ion Source MassHunter GC/MS Acquisition MassHunter and MSD ChemStation data analysis Integration of new Agilent Vacuum Pumps brings intelligent operation Direct Communication between 7890B and 5977A for more productivity 2
3 New Agilent 5977 Series GC/MSD What is the same as 5975? Quality Reliability Proven core technology since 5973/5975 Inert Ion Source Heatable Gold Quartz Quadrupole Triple Axis Detector High Energy Diode Triple Channel EM Detector Productivity Software (MSD ChemStation Data Analysis) 3
4 New Agilent 5977 Series GC/MSD What is new with 5977? Better Performance High Sensitive Extractor Ion Source Improved Agilent Vacuum Pump More Productivity Direct Communication 7890B 5977A Protect system Vent the system faster Power save (Sleep/Wake) MassHunter Software Data Acquisition (Method/Sequence) Data Analysis 4
5 New Extractor Ion Source for 5977 Why the Extractor EI Source? Increase in ion count True increase in sensitivity Better trace level precision Lower IDL and MDL Proven sensitivity increase demonstrated with over Series GC/TQ! Page 5
6 Comparison between Inert Ion Source and Extractor Ion Source Repeller (Push) Ionization area Ions Drawout Plate Extractor Lens (Pull) Ion Focus Lens Entrance Lens Inert Ion Source Mass Filter Extractor Source More ions to Mass Filter Page 6
7 How Extractor Ion source with Etune be seen in the Chromatogram? Blue Extractor 40ppb solution (SVOC) Red Standard 40ppb solution (SVOC) 7
8 More improvements on 5977 s Extractor Ion Source: Improved Thermal Profile Why is this important? Better performance of high boilers i.e. PAH even at lower concentration! Good repeatability of PAH even at the trace level (5ppb)!! 10 Injections 80 min analysis Improvement for better Mass Spectrometer of Gas Chromatograph Page 8
9 Sensitivity Specifications of the 5977 Full Scan EI Specification: S/N - Signal-to-Noise The 5977 in scan mode has some background noise even if only OFN standard is injected, therefore the traditional Signal-to-Noise (S/N) is used as specification. Agilent s 5977 has the highest S/N (1500:1) with Extractor Ion Source among the SQ system. (1pg OFN) SIM Specification: IDL - Instrument Detection Limit Agilent 5977A uses this sensitivity specification for detection techniques where no or little noise is generated, such as when OFN standard is injected. IDL is a new specification for SIM, but have been used with MS/MS for over a year. 9
10 5977A GC/MSD IDL Specification IDL is a statistically derived value from the precision (%RSD) of 8 consecutive, splitless injections of 100fg OFN at 99 % confidence level. The more precise instruments (lower %RSD) will generate lower IDLs. The Agilent 7890B/5977A with an ALS or CTC autosampler has the following specifications: Ion Source type High Vacuum pump type IDL of OFN by 8 replicates of ALS* Extractor Source Turbo Pump 10fg Inert Source Turbo Pump Diffusion Pump 24fg 30fg These are installation specification, performed on customer site, immediately after installation and carry a lot more value than a reference specs. *Systems without an Autosampler will have only S/N specification. 10
11 Why IDL specification is useful for the 5977 in SIM? Influence of Noise in S/N specs In SIM (or MS/MS) when the Noise is too low, such as during acquisition of an OFN standard, S/N results may not give the best characterization If the noise level is insignificant - varies a lot and too low- the derived value from it (S/N) also becomes insignificant Value of IDL IDL is derived from reproducibility/precision results, the same metrics most users utilize every day in their work. The IDL statistical process is the same used for Method Detection Limit calculations, and the %RSD values are tools to monitor analytical performance IDL characterizes the whole system AS, GC, MS- giving confidence in overall reliability. It is the results of 8 consecutive injections, unlike S/N in a single shut, which can be influenced by many parameters/conditions 11
12 The Influence of the low Noise on S/N values Same Data File, RMS, 5 Second Noise Region A 0.01 min. shift in the noise region resulted a 6000 higher S/N value. When the noise is insignificant (low), the derived S/N value is also insignificant! Noise region: 5.65min min S/N: S/N Noise region: 5.66min min S/N: 3335 S/N Noise region: 5.65min-5.73 min Noise region: 5.66min-5.74 min 12
13 Better HW Features: Agilent Turbo Pump (V304) Innovation for: Higher pumping performance especially for He and H 2 High reliability lower cost of operation Agilent Quality Standard TwisTorr 304 FS Press release : Page 13
14 Better HW Features: Agilent Rough Pumps New DS42i High performance Energy saving design Inverter mode not active with diff pump New IDP-3c High performance Oil-free scroll pump NH 3 CI compatible with Agilent Quality Standards Page 14
15 Better HW Features: Power consumption saving of MSD by Inverter Pump (DS42i) What this means to the laboratory cost of ownership? W W 0 Operation mode Sleep Mode 15
16 Example of Cost simulation of saving power with Agilent 7890B/5977A at Environmental Lab Assumptions GC/MSD cycle time: 40 minutes (30min for data acq./10 min for cool down & prep) Sample: 30 samples/day Working day: 5 days/week, no operating on Sat/Sun GC/MSD wake time = 40 min/sample * 30 samples * 5 days/week = 6,000 min/week = 100 hours/week = 32 kwh GC/MSD sleep time = 168 hours/week 100 hours/week = 68 hours/week = 13 kwh Total Power usage (1 week) = 45 kwh (with Sleep mode) Total Power Usage (1 week) = 54 kwh (without Sleep mode Weekly MS Power saving per instrument = 9kWh (17%) 16
17 3 ways to go to Sleep mode From the menu item of MassHunter From the 7890B Keypad From the Sequence Keyword 17
18 Key features of Direct Communication between 7890B and 5977A Sleep/Wake mode In sleep mode, MSD will go to the Power save mode (320W 190W) Laboratory Operating Cost Reduction (see the previous slide for detail) Safety Control If 5977A MSD Pumps fail, 7890B will shut off the gas supply for protecting GC/MSD system. Helium Carrier gas : This prevents wasting gas. Hydrogen Carrier gas: This reduces the risk of explosion in MSD. Cool Down MSD Faster (FastVent) When MSD Vent Cycle started, GC will cool down MSD analyzer at the optimum gas flow to MSD. Less time to wait for cooling down MSD analyzer (40 min 25 min when cooling down from 280C to 100C) Page 18
19 MassHunter GC/MS Acquisition for GC/MSD Unified GC/MS Acquisition and MassHunter Data Analysis; with MSD ChemStation workflow for GC/MSD 19
20 GC/MSD Software MassHunter Qual MH MassHunter Quant CS MassHunter GC/MS Acquisition MSD ChemStation DA And Use Either MassHunter Data Analysis OR MSD ChemStation Data Analysis G1701FA 20
21 The Value of G1701FA Software All Inclusive G1701FA Software Package MassHunter GC/MS Acquisition MSD ChemStation DA MassHunter Quantitative Analysis MassHunter Qualitative Analysis MassHunter Qual MSD ChemStation Data Analysis 21CFR11 Compliance tools (Acquisition and MH Quantitative Analysis) GC/MS and LC/MS Translators Collated User Information Workflow guides, Video Tutorials, all in one location Support 1yr Upgrade, Updates, and Phone Support Training and Familiarization options MassHunter Quant 21
22 MassHunter GC/MSD Rollout Plan Feb 2013: Add GC/MSD systems 5975 & 5977 Series GC/MSD 7890B & 7820A 7697A HSS Late 2013: Expand Compatibility 5973 Network 6890 Network & 6850 GC MH EnviroQuant Continue MassHunter GC/MS and GC support 7000 Series Triple Quad GC/MS 7200 Series Q-TOF GC/MS MH DrugQuant GC and GC/MSD Data Analysis with MassHunter OR MSD ChemStation modes 22
23 ASK When can I start using MassHunter if I buy a 5977 Series GC/MSD? ANSWER Anytime! Without added cost! 23
24 GC/MSD Acquisition Methods MSD Productivity ChemStation MassHunter GC/MS Acquisition GC/MS Acquisition Methods are Transparent from MSD ChemStation to MassHunter GC/MS (Except PAL) 24
25 Migrate MSD ChemStation Data to MassHunter Data MassHunter Quant MSD ChemStation (.D) Data MassHunter Qual WorkStation (.SMS) Data Generic (.CDF) 25
26 Migrate MSD ChemStation Quantitative Methods to MassHunter MassHunter Quant Method MSD ChemStation (QDB.MTH) Method WorkStation (.mth) 26
27 Expanded Capabilities: 7890B/5977A Improving the Industry Standards for GC/SQ Higher Ion Count Extractor EI source Increased ion count S/N > 1500:1 IDL < 10 fg (industry first) Improved thermal profile Better Vacuum by Agilent Pump New Agilent V304 Turbo TwisTorr Molecular Drag technology Improved pumping light gases H 2 (220 L/s); He (255 L/s) Agilent Floating Suspension Longer life Quiet operation Safety/Protection Vacuum failure Shut off gas (He or H2) Save Time & Energy Sequence Scheduler Standby post run Ready to run from standby Bake Mode as needed Factory backflush option Fast Vent capability DS42i rough pump standby power reduction Faster Scanning 20,000 u/s But best choice of fast scanning GC/MS is GC/QTOF 7890B GC Features EMF, calculator, etc. All of 5975 s features also built in 5977, such as Hot Gold Quad, SIM/Scan, Gain tuning, Triple-Axis Detector, Trace Ion Detection, Auto CI, etc.. 27
28 7890B/5977A GC/MS Solution Portfolio Driving Business Value and Success Proven 7890 and 5977 Reliability MassHunter & ChemStation SW Integrated Intelligence Improved Performance Greater Operating Efficiency Expanded Accessories and Options 28
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