WikiPathways Tutorial
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1 WikiPathways Tutorial Mining biological pathways and more Thomas Kelder
2 Wiki for biological pathways Free and open pathway resource Share, curate and discuss!
3 Topics How to Find and filter pathway information Download pathway information Use it in your research Integrate it with other resources
4 Topics Pathway information and curation WikiPathways design & tools Web service Real life examples
5 Pathway information and curation
6 Biological pathways Organize our knowledge about biology Using graphics: Intuitive representation of complex systems Facilitates communication and discussion Visualization of experimental data
7 Organize knowledge Insulin inactivates liver phosphorylase, the principal enzyme that causes liver glycogen to split into glucose. This prevents breakdown of the glycogen that has been stored in the liver.
8
9 How pathway information is used Data visualization Knowledgebase Network analysis Enrichment analysis
10 Challenges of curating pathways Not just an image Relations Annotations Literature references
11 Challenges of wikifying pathways Most wiki engines are designed for textual information Our pathways are stored as XML and rendered using Java libraries
12 WikiPathways design & pathway tools
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17 WikiPathways data model Pathway identifiers Unique & stable WP1, WP43, WP1373 Curation tags provide quality annotations All other information is stored in GPML
18 Working with the GPML format
19 Working with the GPML format XML format, platform independent PathVisio Java library org.pathvisio.model Pathway A GPML pathway - readfrom/writetoxml Read/write GPML - writetosvg Convert to image - getdataobjects Get the pathway elements - add/remove Add/remove elements PathwayElement An element on the pathway - getobjecttype DataNode, Line, Shape - get/settextlabel Get or set GPML properties org.pathvisio.view Render the pathway in your own application!
20 Working with annotations DataNodes are annotated with an Xref DataSource Identifier Ensembl ENSG Entrez Gene 675 PubChem 8422 Which DataSource to use is up to the user Pathway 1 Ensembl ENSG ? Pathway 2 Entrez Gene 675
21 Working with annotations WikiPathways provides functions where the identifiers have been mapped for you Use BridgeDB library for solving your own mapping problems
22 Putting it all together MediaWiki extensions Web service API Validation Pathway rendering Page rendering WikiPathways PHP layer Pathway model MediaWiki MediaWiki DB Metadata cache BridgeDb Gene + metabolite mappings Editor applet PathVisio Lucene indexer Java components
23 WikiPathways Web service Web service Website
24 Web service
25 SOAP Simple Object Access Protocol How your code talks to WikiPathways XML-based Platform independent Language independent
26 Request Response
27 WSDL Web Services Description Language Defines: Function signatures Data structures Automatic client method/class generation
28
29 REST Representational State Transfer URL based, HTTP requests Not bound to XML Platform independent Language independent
30
31 Finding pathways Get all pathways / organisms listpathways listorganisms Pathways with text apoptosis findpathwaysbytext Pathways containing Glucose (CHEBI:17634) findpathwaysbyxref Pathways citing Lakin et al., Oncogene 1999 findpathwaysbyliterature
32 Downloading pathways Get the pathway title, species, last revision getpathwayinfo Get the GPML getpathway Other formats (SVG, PDF, PNG) getpathwayas Mapped identifier list getxreflist Color gene boxes getcoloredpathway
33 Wiki information Get the revision history getpathwayhistory Recently changed pathways getrecentchanges Curation tags (e.g. quality annotations) getcurationtags
34 Alternatives to the web service Download all pathways as GPML or image Text file with info on all pathways
35 Basic examples
36 Java example Use the wikipathways-client library org.pathvisio.wikipathways.wikipathwaysclient
37 Find pathways
38 Extract pathway information org.pathvisio.model.pathway Automatic GPML parsing
39 List genes, proteins and metabolites
40 Advantages of using Java We provide a high-level API No need to deal with SOAP/WSDL Compatible with PathVisio Easier GPML handling Compatible with BridgeDb Faster and customizable identifier mapping
41 SSOAP R Example
42 List available organisms
43 Real life examples
44 Demo: Cytoscape listorganisms findpathwaysbytext getpathway
45 Automate curation tasks Propose to clean up test/tutorial pathways
46 Automate curation tasks List all tutorial pathways getcurationtagsbyname Check if they have recently been edited getpathwayhistory If not, add proposed deletion tag savecurationtag
47 Enrichment analysis in R Get all human pathways as mapped gene lists getxreflist Download a GEO dataset Perform Parametric Gene Set Enrichment ~75 LOC (including comments)
48 Step 1a: List all human pathways
49 Step 1b: List all human pathways
50 Step 2: Create gene sets
51 Step 3: Enrichment analysis
52
53 SNPLogic Provide biological context for SNPs SNPs -> Genes -> Pathways Uses functions: findpathwaybyxref getcoloredpathway
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57 Wikipedia integration
58 Static image Link to Wikipedia gene page Interactive image! No gene page exists, link to create new page
59
60 Each blue line is a <div> display:block <div style="display:block; width:60px; height:0px; overflow:hidden; position:relative; left:502.0px; top:181.3px; background:transparent; border-top:3px blue solid"></div>
61 Each which blue fits line into is an a Annotation <div> display:block template {{Annotation 0 0 [[<div>]]}}
62 There is one for each line, plus a special Each which blue fits line into is an a switch statement to Annotation <div> display:block template highlight one gene per article. {{Annotation 0 0 [[<div>]]}} {{Annotation 0 0 [[<div>]]}} {{Annotation 0 0 [[<div>]]}} {{#switch:{{{highlight}}} PDHA1={{Annotation 0 0 [[<div>]]}} PDHA2={{Annotation 0 0 [[<div>]]}} PDHB={{Annotation 0 0 [[<div>]]}} }}
63 There is one for each All of this is added to line, plus a special an Each which Annotated blue fits line into Image is an a switch statement to Annotation template <div> display:block along template with highlight one gene the image map itself. per article. {{Annotated image <image map> {{{{[[<div>]]}}}}}}
64 There is Annotated one for each All of this is added to Image line, plus is added a special to a an Each which Annotated blue fits line into Image is an a switch Preview statement Crop to Annotation template <div> display:block along template with highlight template one with gene its the image map itself. own per switch article. cases. {{Preview crop Image={{Annotated image <> {{{{[[<>]]}}}}}} cwidth={{#switch:{{{highlight}}} }} cheight={{#switch:{{{highlight}}} }} otop={{#switch:{{{highlight}}} }} oleft=={{#switch:{{{highlight}}} }} Description}}
65 There is Annotated one for each All Finally, of this all is of added this is to Image line, plus is added a special to a an put Each which Annotated into blue fits a <div> line into Image is an tag a switch Preview statement Crop to Annotation template <div> to control display:block along full-size template with highlight template one with gene its the width image and map height. itself. own per switch article. cases. <div style= >{{Preview crop {{<> {{{{[[<>]]}}}}}}}}</div>
66 Template usage
67 Demo: Taverna workflows - Include pathway information in Taverna workflows. - Get basic workflows you can use as building blocks at:
68 Help -> Web service Example applications and source code API Documentation Links to useful libraries Also see: PathVisio library for handling GPML BridgeDb library for identifier mapping
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