MicrosEmi CRP External Output Manual (English)

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2 Miyanohigashi, Kisshoin Minami-ku kyoto 601-8510 Japan EC

1 MicrosEmi CRP External Output Manual (English) CODE:I GZ HORIBA. Ltd. 2 Miyanohigashi, Kisshoin Minami-ku kyoto Japan EC REP HORIBA ABX S.A.S. Rue du Caducée - Parc Euromédecine MONTPELLIER Cedex 4 - France

2 Revisions Part number Technical Note Software revision Sections Date All 31/08/2010 This document applies to the latest software version as indicated above. When a subsequent software version changes the information in this document, a new section and/or sections will be released. Declaration of conformity: Latest version of the CE declaration of conformity for this instrument is available on Notice of liability: The Information in this manual is distributed on an "As Is" basis, without warranty. While every precaution has been taken in the preparation of this manual, HORIBA ABX will not assume any liability to any persons or entities with respect to loss or damage, caused or alleged to be caused directly or indirectly by not following the instructions contained in this manual, or by using the computer software and hardware products described herein in a manner inconsistent with our product labeling. Graphics: All graphics including screens and printouts, photographs are for illustration purposes only and are not contractual. Trademarks: Other product names mentioned within this publication may be registered trademarks of other companies. Copyright 2010 by HORIBA ABX All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of HORIBA ABX. HORIBA ABX Rue du Caducée - Parc Euromédecine MONTPELLIER Cedex 4 - FRANCE Tel: (33) (0) Fax: (33) (0)

3 Manual Information Description of codes $xx (x is alpha-numeric character) indicates hexadecimal binary code. This manual expresses the codes as follows. Code Notation $0D <CR> $0A <LF> $02 <STX> $03 <ETX> $20 (space) $2C, (comma) Other characters no listed above are indicated with ASCII characters. Description in this manual Note Provides necessary knowledge for proper operation and additional information on handling the instrument. Reference Shows sections to which you can refer for additional information. Tip Provides useful information about operation. Symbols used in figures Shows the key to be operated. Shows the section which requires attention.

5 Contents 1 Compact flash memory (CF) CF restrictions Inserting and removing a CF CF handling precautions To insert a CF To remove a CF Preparing a CF (formatting) Saving data to a CF To save data Created files Data formats Common format Measurement data format QC measurement data (common to N, H, L) CBC CAL measurement data CRP CAL measurement data XB data Loading data from a CF RS-232C Instrument connector and communication cable Connecting the instrument to a PC or other device RS-232C settings Output Measurement result output Quality control measurement (QC) result output Outputting measurement data via data communication Outputting quality control measurement (QC) data via data communication Output format Measurement results and test output Description of format Connecting to a network Network connection precautions and restrictions Instrument connector and communication cable Connecting the instrument to a PC or other device Network settings

6 3.5 Connecting to a client Output format type and protocol Output Output format

7 1 Compact flash memory (CF) 1 Compact flash memory (CF) This instrument has a compact flash memory (CF) slot and a memory loader. You can transfer instrument data as a CSV format file to a CF and copy the data to a PC from the CF. You will need a CF card reader or other device in order to copy data from a CF card to a PC. Compact flash memory (CF) slot Note Do not use a CF as data backup media. The data save operation always saves data using the same file names. This means the newly saved data overwrites previously existing data, making the previous data is no longer available. 1.1 CF restrictions Never insert or remove a CF while the instrument is starting up. Doing so can damage the CF. Never insert or remove a CF in the following cases. Doing so can damage the CF. While "Formatting in progress" is displayed. While "Data Saving in progress" is displayed. While data is being loaded from a CF. Use only compact flash memory specified by HORIBA. Use of other, non-specified compact flash memory can cause improper operation. You can store data to a CF only up to the capacity specified for the instrument. Exceeding the capacity specified for the instrument will make it impossible to access older data. Do not change the file names of the data created on the CF. Data is saved to a CF by overwriting previous data. Changing a data file name will cause a new data file to be created, and, if the CF memory capacity is exceeded, you will not be able to save any more data. Do not use a data save CF for long-term data storage. For long-term storage of data saved to a CF, copy the data to a PC hard disk or other media. 1

1 Compact flash memory (CF) 1.2 Inserting and removing a CF 1.2.1 CF handling precautions Insert and remove CF cards slowly and carefully.

8 1 Compact flash memory (CF) 1.2 Inserting and removing a CF CF handling precautions Insert and remove CF cards slowly and carefully. Inserting and removing a CF more than necessary can cause a CF alert to occur To insert a CF 1. With the CF oriented as shown below, gently insert it straight into the CF slot until it stops. Compact flash memory (CF) Compact flash memory (CF) slot 2. Press down on the CF until its top edge is flush with the top of the unit (1). The eject lever will come out (2) when the CF is fully inserted. Fold down and store the eject lever as shown below (3). 1 2 Eject lever 3 2

1 Compact flash memory (CF) 1.2.3 To remove a CF 1.

This will cause the CF to eject part way from the instrument.

9 1 Compact flash memory (CF) To remove a CF 1. Check to make sure that the message "Formatting in progress" or "Data Saving in progress" is not displayed on the instrument screen. 2. As shown below, unfold the eject lever (1) and then press it straight down (2) This will cause the CF to eject part way from the instrument. pick the CF with your fingers and pull it up to remove it. Note Blood, chemicals, or other foreign matter soiling the CF slot can lead to damage of the instrument. Should the CF slot become soiled, contact your HORIBA sales representative. 3

10 1 Compact flash memory (CF) 1.3 Preparing a CF (formatting) You must format (initialize) a CF before using it for the first time. 1. Insert the CF into the CF slot as described under "1.2 Inserting and removing a CF" (page 2). 2. Perform the following operation: [MENU] -> [The Other Functions] -> [Maintenance] -> [CF] -> [Format]. A confirmation screen will appear. Press [OK] to start the CF formatting (initialization) operation. The message "Formatting in progress" will appear on the display. Note Never remove the CF while the message "Formatting in progress" is on the display. Doing so can damage the CF. Formatting (initialization) is complete when the screen returns to "CF". 4

1 Compact flash memory (CF) 1.4 Saving data to a CF You can use the following procedure to save data recorded by the instrument to a CF. 1.4.1 To save data 1.

11 1 Compact flash memory (CF) 1.4 Saving data to a CF You can use the following procedure to save data recorded by the instrument to a CF To save data 1. Insert the CF into the CF slot as described under "1.2 Inserting and removing a CF" (page 2). 2. Perform the following operation: [MENU] -> [The Other Functions] -> [Maintenance] -> [CF] -> [Data save]. A confirmation screen will appear. Press [OK] to start the data save. The message "Data Saving in progress" will appear on the display. Note Never remove the CF while the message "Data Saving in progress" is on the display. Doing so can damage the CF. Data save will not be performed correctly if the CF does not have enough capacity to store the data or if the CF card is not inserted correctly. (In this case, a C95 alert will appear on the display.) Data save is complete when the screen returns to "CF". 5

12 1 Compact flash memory (CF) Created files Performing a data save operation creates the following seven CSV files and one folder on the CF. CSV files MES_DATA.CSV: Measurement data CBC measurements, +CRP measurements QCN_DATA.CSV: Quality control measurement data QCN CRP, QCN CBC (Or QC1 CRP, QC1 CBC) QCH_DATA.CSV: Quality control measurement data QCH CRP, QCH CBC (Or QC2 CRP, QC2 CBC) QCL_DATA.CSV: Quality control measurement data QCL CRP, QCL CBC (Or QC3 CRP, QC3 CBC) XB_DATA.CSV: XB data CAL_DATA.CSV: CBC calibration measurement data CAL_CRP.CVS: CRP calibration measurement data Folder SYSTEM: (Folder for storage of service data.not normally used for daily operation.) 6

13 1 Compact flash memory (CF) 1.5 Data formats This section explains the format of each file Common format Common format of each CSV file Data is output in CSV format. Data delimiter:, (single-byte comma) Each measurement is output as one line, with new line codes at the end: <CR><LF> Measurement data format Line 1 Instrument serial number Newline xxxxxxxx<cr><lf> Line 2 and subsequent lines ID Measurement date and time Measurement value (19 items) Flag Specimen container Newline xxx...xxx,yyyy/mm/dd hh:mm,xx.x,x,x,~,xx.x,x,x,l1,m2,g1,g2,g3,mic,sch,scl,xx.x,x,x,...<cr><lf> ~ Items... WBC PLT * Not used ID Specified by a text string up to 20 characters long. The actual length depends on the ID setting, and may be less than 20 characters. Measurement date and time Outputs the measurement date and time in the specified format. Refer to " Setting the date display format" in the MicrosEmi CRP User Manual. The following are the initial factory default settings. Year Month Day Hour Minute YYYY/MM/DD hh:mm Space Measurement value The following 19 items are output: WBC, RBC, Hgb, Hct, MCV, MCH, MCHC, RDW, PLT, MPV, PCT, PDW, %LYM, %MON, %GRA, #LYM, #MON, #GRA, CRP. The three items described below are output for each measurement value. xx.x,x,x Measurement value Flag 1 Flag 2 The number of digits depends on the instrument's unit setting. Measurement values are output using the same number of digits as appear on the display. U, D, $, *, B, T, E, F, X, C, Space H, L, Space Specimen container Outputs the specimen container used for the measurement. 0 None 1 Standard sample tube 2 Not normally used. 3 Sample cup 7

14 1 Compact flash memory (CF) Flag Outputs the status of the WBC flag and PLT flag. A space is output when there is no flag. WBC flag The following five items are output for this flag. L1, M2, G1, G2, G3 PLT flag The following three items are output for this flag. MIC, SCH, SCL QC measurement data (common to N, H, L) Line 1 Instrument serial number Newline xxxxxxxx<cr><lf> Line 2 and subsequent lines ID Measurement date and time Measurement values (17 items) Specimen container Newline xxx...xxx,yyyy/mm/dd hh:mm,xx.x,x,x,~,xx.x,x,x,,,,,,,,,xx.x,x,x,...<cr><lf> ~ Items... * Not used ID Specified by a text string up to 20 characters long. The actual length depends on the ID setting, and may be less than 20 characters. Measurement date and time Outputs the measurement date and time in the specified format. Refer to " Setting the date display format" in the MicrosEmi CRP User Manual. The following are the initial factory default settings. Year Month Day Hour Minute YYYY/MM/DD hh:mm Space Measurement value The following 17 items are output: WBC, RBC, Hgb, Hct, MCV, MCH, MCHC, RDW, PLT, MPV, %LYM, %MON, %GRA, #LYM, #MON, #GRA, CRP. The three items described below are output for each measurement value. xx.x,x,x Measurement value Flag 1 Flag 2 The number of digits depends on the instrument's unit setting. Measurement values are output using the same number of digits as appear on the display. U, D, $, *, T, E, F, X, C, Space H, L, Space 8

15 1 Compact flash memory (CF) Specimen container Outputs the specimen container used for the measurement. 0 None 1 Standard sample tube 2 Not normally used. 3 Sample cup CBC CAL measurement data Line 1 Instrument serial number Newline xxxxxxxx<cr><lf> Line 2 and subsequent lines ID Measurement date and time Measurement values (5 items) Specimen container Newline xxx...xxx,yyyy/mm/dd hh:mm,xx.x,x,x,~,xx.x,x,x,,,,,,,,,xx.x,x,x,...<cr><lf> ~ Items... * Not used ID Specified by a text string up to 20 characters long. The actual length depends on the ID setting, and may be less than 20 characters. Measurement date and time Outputs the measurement date and time in the specified format. Refer to " Setting the date display format" in the MicrosEmi CRP User Manual. The following are the initial factory default settings. Year Month Day Hour Minute YYYY/MM/DD hh:mm Space Measurement value The following five items are output for this flag: WBC, RBC, Hgb, Hct, PLT. The three items described below are output for each measurement value. xx.x,x,x Measurement value Flag 1 Flag 2 Output is in (STD) units, regardless of the unit setting. Measurement values are output using the same number of digits as appear on the display. Flag 1 and Flag 2 are output as spaces. Specimen container Outputs the specimen container used for the measurement. 0 None 1 Standard sample tube 2 Not normally used. 3 Sample cup 9

16 1 Compact flash memory (CF) CRP CAL measurement data Line 1 Instrument serial number Newline xxxxxxxx<cr><lf> Line 2 and subsequent lines ID Measurement date and time Measurement value Specimen container Newline xxx...xxx,yyyy/mm/dd hh:mm,xx.x,x,x,xx.x,x,x,...<cr><lf> (Line 1) * Not used ID Specified by a text string up to 20 characters long. The actual length depends on the ID setting, and may be less than 20 characters. Measurement date and time Outputs the measurement date and time in the specified format. Refer to " Setting the date display format" in the MicrosEmi CRP User Manual. The following are the initial factory default settings. Year Month Day Hour Minute YYYY/MM/DD hh:mm Space Measurement value The following is output for the first CRP item. The three items described below are output for each measurement value. xx.x,x,x Measurement value Flag 1 Flag 2 Output is in (STD) units, regardless of the unit setting. Measurement values are output using the same number of digits as appear on the display. Flag 1 and Flag 2 are output as spaces. Specimen container Outputs the specimen container used for the measurement. 0 None 1 Standard sample tube 2 Not normally used. 3 Sample cup 10

17 1 Compact flash memory (CF) XB data Line 1 Instrument serial number Newline xxxxxxxx<cr><lf> Line 2 and subsequent lines Batch start DATE&TIME Batch end DATE&TIME Operation value (Item 3) YYYY/MM/DD hh:mm,yyyy/mm/dd hh:mm,xx.x,xx.x,xx.x,<cr><lf> Items... Batch start DATE&TIME/Batch end DATE&TIME Outputs the measurement date and time in the specified format. Refer to " Setting the date display format" in the MicrosEmi CRP User Manual. The following are the initial factory default settings. Year Month Day Hour Minute YYYY/MM/DD hh:mm Space Newline Operation value The following three items are output: MCV, MCH, MCHC. Only the operation value is output for each item. No flag is output. xx.x Operation value Output is in (STD) units, regardless of the unit setting. Measurement values are output using the same number of digits as appear on the display. 11

18 1 Compact flash memory (CF) 1.6 Loading data from a CF You can use the following procedure to load data recorded on a CF into the instrument. Loadable data is control blood assay value/tolerance (assay value file). Note A control blood assay value file may be loaded only when conducting control management using control blood of different concentrations: N, H, L. Refer to "9.1 Control status" in the MicrosEmi CRP User Manual. Contact your HORIBA sales representative for information on how to obtain assay value files. 1. Insert the CF that contains the control blood assay value files into the CF slot as described under "1.2 Inserting and removing a CF" (page 2). 2. Perform the following operation: [MENU] -> [Quality Control] -> [New Control Setup] -> [Load from CF]. This will display a screen showing how to load the assay value file from the CF. Note When control blood N is specified for control management, executing [Load from CF] will display a list of the control N LOT. 3. Select the assay value file load method and load the data. 12

1 Compact flash memory (CF) Batch loading This operation batch loads the control N, H, and L assay value/tolerance data of the same LOT from the assay value files on the CF.

19 1 Compact flash memory (CF) Batch loading This operation batch loads the control N, H, and L assay value/tolerance data of the same LOT from the assay value files on the CF. On the [Load from CF] screen, select [Load Three Lots] to display a list of control blood LOTs whose assay value files are on the CF. Use the [ ] and [ ] to select the LOT you want to load and then press [Load]. This will load the control N, H, L assay value/tolerance data into the instrument. Control N, H, L This operation loads specific control N, H, or L assay value/tolerance data from the assay value files on the CF card. By way of example, here we will show how to load the assay value/tolerance data for control N. To load other control assay value/tolerance data, replace "N" in the explanation text with "H" or "L". On the [Load from CF] screen, select [Control N] to display a list of control N LOTs whose assay value files are on the CF. Use the [ ] and [ ] to select the LOT you want to load and then press [Load]. This will load the assay value/tolerance data into the instrument. Note Never insert or remove a CF while data is being loaded from it. Doing so can damage the CF. 4. Data loading is complete when the "Load from CF" screen returns to the display. 13

20 2 RS-232C 2 RS-232C This instrument supports RS-232C data communication protocol, which allows it to output the measurement data it displays to a PC or other device. Note Data communication does not support output of the following types of data: individual calibration measurement data, individual calibration operation table values, individual quality control operation table values, the LJ graph, individual XB operation table values, XB data. 2.1 Instrument connector and communication cable Instrument connector D-sub 9 pins (male plugs, inch fastening screws) Communication cable It is up to you to provide a shielded crossover cable no greater than 1.5 meters long with D- sub 9 pins connectors (male receptacle, inch fastening screws) on both ends. 2.2 Connecting the instrument to a PC or other device To connect the instrument to a PC or other device 1. Make sure that the instrument and the PC or other device are turned off. 2. Connect one of the connectors of the communication cable (described in 2.1) to the RS-232C terminal on the bottom-left of the back of the instrument. 3. Connect the other connector of the communication cable to the RS-232C terminal on the back of the PC or other device. The following shows a sample connection with a common PC. MicrosEmi CRP Connector: D-sub 9 pins Signal name Pin No. RxD 2 TxD 3 SG 5 PC Connector: D-sub 9 pins Pin No. Signal name 2 RxD 3 TxD 5 SG 14

21 2 RS-232C 2.3 RS-232C settings Setting method Communication conditions Data rate: 9600 bps; Data length: 8 bits; Stop bit: 1; Parity: None One-way communication only, from the instrument to the PC or other device. Flow control and handshake are not performed. Configuring instrument settings (Refer to " RS-232C data output settings" in the MicrosEmi CRP User Manual.) Perform the following operation to turn on the RS setting: [Menu] -> [The Other Functions] -> [Settings] -> [Function 1] -> [RS] -> [ON]. 15

22 2 RS-232C 2.4 Output Measurement result output A measurement result is output via data communication when is appears on the instrument screen. The currently displayed measurement result also can be re-output via data communication by pressing [PRINT] on the measurement result screen. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND] Quality control measurement (QC) result output Pressing the [OK] key on the screen following a quality control measurement (QC) will output the quality control measurement result via data communication. The currently displayed measurement result also can be re-output via data communication by pressing [PRINT] on the measurement result screen. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. 16

23 2 RS-232C Outputting measurement data via data communication You can use the following operation to output measurement data recorded by the instrument via data communication. [MENU] -> [Results] -> [Day's Results] (Refer to "8.1 Day's results" in the MicrosEmi CRP User Manual.) You also could use the following operation. [MENU] -> [Results] -> [All Results] (Refer to "8.2 All results" in the MicrosEmi CRP User Manual.) Pressing [PRINT] on the measurement result screen will output the data via data communication. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. 17

24 2 RS-232C Outputting quality control measurement (QC) data via data communication You can use the following operation to output via data communication quality control measurement (QC) data recorded by the instrument. Example: Control N [Menu] -> [Quality Control] -> [Search Results] -> [Control N] -> (Refer to "9.4 Search results" in the MicrosEmi CRP User Manual.) Pressing [PRINT] while individual quality control measurement data is displayed will output the data via data communication. The procedure for control H and control L is the same as above. Tip Control N, H, and L are indicated as 1, 2, and 3 by the display format setting. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. 18

2 RS-232C 2.5 Output format The output format is based on HORIBA's original ABX format. 2.5.1 Measurement results and test output Format for one data item (when unit setting is STD) *: Quality control measurement (QC mode) does not have a PCT or PDW item.

25 2 RS-232C 2.5 Output format The output format is based on HORIBA's original ABX format Measurement results and test output Format for one data item (when unit setting is STD) *: Quality control measurement (QC mode) does not have a PCT or PDW item. There is no CRP item in the CBC mode or QC (CBC) mode. Header The number of items depends on the mode. Footer x: Number or character fl: One-character repeatability flag FL: One-character other flag 19

26 2 RS-232C Output example <STX> 00776<CR> $FF RESULT <CR> p 5A<CR> q 06/05/17 10h30mn49s<CR> u AUTO_SID001<CR> s 0002<CR> v t M<CR> $80 D<CR>! <CR> <CR> l<cr> <CR> <CR> l<cr> l<cr> <CR> A <CR> B <CR> C <CR> # l<cr> % l<cr> ' h<cr> " l<cr> $ l<cr> & h<cr> K h<cr> W $xx(x 128 pcs)<cr> X $xx(x 128 pcs)<cr> Y $xx(x 128 pcs)<cr> S <CR> _ 040<CR> P <CR> ] F 014<CR> $FB LC 667 <CR> $FE V5.1 <CR> $FD 9F16<CR> <ETX> <CR> 20

27 2 RS-232C Description of format Identifiers used by headers and footers Configuration Identifier Description Format Number of bytes None Packet length Text string (*1) 5+1 $FF Packet type Text string (*2) $FB Instrument name Text string (*3) $FE Version number Text string (*4) $FD Checksum value 4-byte hexadecimal number (*5) *1: Packet length is the total number of characters excluding <STX> and <ETX>. *2: When a packet type character string that is less than eight characters is sent, it is packed with spaces on the right. Packet type information can specify message content as a measurement result or quality control measurement (QC). Test output performs output of an example that is the same format as the measurement result in order to confirm that RS-232C output can be performed normally. Packet type RESULT QC-RES-M QC-RES-L QC-RES-H TEST-RES Application Measurement result Quality control measurement result (QCN or QC1) Quality control measurement result (QCL or QC2) Quality control measurement result (QCH or QC3) Test output *3: Instrument name Outputs the instrument name during data communication (Example: "LC 667 "). When a character string that is less than eight characters is sent, it is packed with spaces on the right. *4: Version number Outputs an ABX format version number (Example: "V5.1 "). *5: Checksum value A byte-wise add is performed of all data except for <STX>, <ETX>, and the checksum. The checksum value is the lower two bytes of the result expressed as four ASCII characters that represent a hexadecimal number. 21

28 2 RS-232C Identifiers for specimen and other information Configuration Identifier Description Example Format $70 (p) Instrument number (last two digits of serial number) $71 (q) Measurement date and time $75 (u) ID (specimen number) $73 (s) Number of measurements per day (normal measurements only) Number of bytes 5A Text string /01/05 13h15mn31s (*6) ABCD-5555 (*7) Text string Text string Text string *6: Regardless of the date format setting, the format of the measurement date and time is: YY/MM/DD HHhMMmnSSs. *7: When an ID (specimen number) character string that is less than 20 characters is sent, it is packed with zeros (0) on the left. Measurement result Configuration The measurement result consists of a one-character identifier and one space, followed by a five-digit value, two flag characters, and <CR>. Identifier Description Example Format Number of bytes $21 (!) WBC 007.4R Text string $32 (2) RBC h Text string $33 (3) Hgb l Text string $34 (4) Hct Text string $35 (5) MCV h Text string $36 (6) MCH l Text string $37 (7) MCHC Text string $38 (8) RDW l Text string $40 (@) PLT 00401S Text string $41 (A) MPV Text string $42 (B) PCT Text string $43 (c) PDW Text string $23 (#) LYM (%) 027.4R Text string $25 (%) MON (%) 009.4R Text string $27 (') GRA (%) 009.4R Text string $22 (") LYM (#) 002.0R Text string $24 ($) MON (#) 000.7R Text string $26 (&) GRA (#) 000.7R Text string $48 (K) CRP h Text string Remarks (Not output in the QC (CBC) or QC (CRP) mode.) (Not output in the QC (CBC) or QC (CRP) mode.) Not output in the CBC mode or QC (CBC) mode. 22

29 2 RS-232C Values The value format is five places, including the decimal point. When a value that is less than five places is sent, it is packed with zeros (0) on the left. The unit setting for the above table is "STD (Standard)". Changing the unit setting will cause the decimal point position to change accordingly. (In the QC (CBC) and QC (CRP) mode, the "STD (Standard)" unit is always used for output, even if the unit setting is changed.) Items for which a numeric value is not obtained are output as "-----". Flags The sixth character (fl) following the five-place value is a repeatability flag, and the seventh character (FL) is the "other" flag. fl flag Description Repeatability in the measurement result (Space) S Repeatability flag. Indicated as "$" in screen and print results. R Repeatability flag. Indicated as "*" in screen and print results. FL flag (Space) h I O U z T e F X C Description Measurement result falls within the management range. Measurement result is above the management range. Indicated as "H" on screen and printed output. Measurement result is below the management range. Indicated as "L" on screen and printed output. Measurement result exceeds the measurement range. Indicated as "D" on screen and printed output. Measurement result is less than the measurement range. Indicated as "U" in screen and print results. Blank alert flag. Indicated as "B" in screen and print results. Temperature alert flag. Indicated as "T" in screen and print results. CRP reagent empty or CRP reagent sensor error. Indicated as "E" in screen and print results. CRP count acquisition failure. Indicated as "F" in screen and print results. CRP measurement result by expired CRP reagent. Indicated as "X" in screen and print results. CRP measurement result by incorrect CRP reagent. Indicated as "C" in screen and print results. Example: Unit: "Standard"; RBC measurement value: 5.5; Repeatability flag produced R <CR> PLT, WBC flags These flags are displayed following a (blank space). These flags are replaced by spaces when no flag is detected. Example: S PcSc <CR> P L1 M2 G2 <CR> Identifier Measurement item Format Number of bytes $53 (S) PLT flag PcScMc $50 (P) WBC flag L1 M2G1G2G Pc --- SCL Sc --- SCH Mc --- MIC 23

30 2 RS-232C WBC, RBC, PLT histogram data Following a space, histogram data is sent as 128 bytes (128 horizontal-axis cell size channels and one-byte of frequency data for each channel). The following is the full scale of the horizontal (cell size) axis. WBC: 450 fl RBC: 300 fl PLT: 40 fl The full scale of the vertical (frequency) axis is calculated and updated automatically to allow the maximum frequency of the horizontal channels to be 223. The frequency will be 0 when the value of the vertical axis is 32, and a maximum frequency of 223 when the vertical axis is 255. Identifier Measurement item Format Number of bytes $57 (W) WBC Horizontal channel frequency $58 (X) RBC Horizontal channel frequency $59 (Y) PLT Horizontal channel frequency WBC, PLT threshold values These threshold values are indicated by the histogram horizontal (cell size) channel number (decimal value). Each threshold value is sent as a space followed by three bytes. Identifier Measurement item Format Number of bytes xxx xxx $5D (]) WBC threshold value $5F (_) PLT threshold value (1) (2) (1) LYM-MON threshold value (2) MON-GRA threshold value One maximum threshold value for calculation of PLT WBC In order to perform three WBC differential counts, there are two types of threshold values: LYM (lymphocyte) and MON (monocyte) threshold value, and MON (monocyte) and GRA (granulocyte) threshold value. LYM (lymphocyte) and MON (monocyte) threshold value MON (monocyte) and GRA (granulocyte) threshold value PLT The maximum channel used to calculate PLT is set as the threshold value. Maximum channel used to calculate PLT 24

31 3 Connecting to a network 3 Connecting to a network This instrument supports IEEE802.3 protocol Ethernet connection, which allows it to output the measurement data it displays to a PC or other device. You also can access and download measurement data from a PC or other device. 3.1 Network connection precautions and restrictions To prevent information theft, connect only with a private network address or other protected network environment. Server function is not supported now. Set the connection to [CLIENT]. This instrument is not equipped with security functions. HORIBA assumes no responsibility for any damages due to information leaks. Use this instrument only in a protected network environment. Connection to a network should be performed by a person with network knowledge and with the approval of the network administrator. 3.2 Instrument connector and communication cable Instrument connector RJ-45 Communication cable Ethernet twisted pair cable (Category 5 UTP cable) Note Use a cable length that does not exceed 100 meters. 3.3 Connecting the instrument to a PC or other device To connect the instrument to a PC or other device 1. Make sure that the instrument and the PC or other device are turned off. 2. Connect the other end of the communication cable, which is connected to a network that satisfies the conditions in section 3.1, to the Ethernet connector located in the middle of the back of the instrument. Connector pins Signal name Signal direction 1: TD+ OUT 2: TD OUT 3: RD+ IN 4: NC - 5: NC - 6: RD IN 7: NC - 8: NC - 25

3 Connecting to a network 3.4 Network settings (Refer to "11.2.6 Network settings" in the MicrosEmi CRP User Manual.

32 3 Connecting to a network 3.4 Network settings (Refer to " Network settings" in the MicrosEmi CRP User Manual.) Perform the following operation: [Menu] -> [The Other Functions] -> [Settings] -> [Function 2] -> [Network]. Next, configure the settings of the items described below. Note To enable network settings you configure, you must turn the instrument off and then back on again. After configuring settings, perform the required exit procedure and then turn off the power switch on the back of the instrument. Next, turn the power switch back on again. Connection SERVER This function is not supported now. CLIENT With the instrument as a client, connects an access point host (PC or other device) as the server in accordance with TCP/IP and individually determined protocol. BOTH This function is not supported now. IP Address Specifies the IP address assigned by your network administrator to the instrument. Subnet Mask Check with your network administrator concerning this setting. If you do not need to use this setting, specify (which is the initial factory default). Default Gateway Check with your network administrator concerning this setting. 26

33 3 Connecting to a network Host PC IP Address Check with your network administrator concerning this setting. It specifies the IP address of the PC or other device that will be the access point host server. Host PC Port No. Check with your network administrator concerning this setting. It specifies the port number of the PC or other device that will be the access point host server when [CLIENT] or [BOTH] is specified for the Connection setting. 27

) A connection will be established when data communication starts and terminated when one data send is complete.

operation table values, the LJ graph, individual XB operation table values, XB data.

34 3 Connecting to a network 3.5 Connecting to a client When the Connection setting of the instrument's network settings is [CLIENT] or [BOTH], the instrument will connect as a client to the access point host server (PC, etc.) A connection will be established when data communication starts and terminated when one data send is complete. Note Data communication does not support output of the following types of data: individual calibration measurement data, individual calibration operation table values, individual quality control operation table values, the LJ graph, individual XB operation table values, XB data. Data communication will not be performed correctly if network settings are configured incorrectly, or if the LAN cable between the connected PC and other device is not connected properly. (When a problem occurs, the C85 alert will appear on the display.) Output format type and protocol Output format You can select either ASTM format or ABX format. Output conforms to ASTM E1394 when ASTM format is selected. Output conforms to HORIBA's original ABX format when ABX format is selected. Histogram data can be output with ABX format. Protocol Protocol is TCP/IP. While ASTM format is selected, a connection is established for each data item, the data item is sent line-by-line, and then the data connection is terminated when output is complete. While ABX format is selected, a connection is established for each data item, the data item is sent as a single packet, and then the data connection is terminated when output is complete. Selecting the output format Perform the following operation: [Menu] -> [The Other Functions] -> [Settings] -> [Function 1] -> [Output Format] -> [LAN client] -> Select [ABX] or [ASTM]. -> Press [OK]. 28

35 3 Connecting to a network Output Measurement result output A measurement result is output via data communication when it appears on the instrument screen. The currently displayed measurement result also can be re-output via data communication by pressing [PRINT] on the measurement result screen. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. Quality control measurement (QC) result output Pressing the [OK] key on the screen following a quality control measurement (QC) will output the quality control measurement result via data communication. The currently displayed measurement result also can be re-output via data communication by pressing [PRINT] on the measurement result screen. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. 29

3 Connecting to a network Outputting measurement data via data communication You can use the following operation to output measurement data recorded by the instrument via data communication.

36 3 Connecting to a network Outputting measurement data via data communication You can use the following operation to output measurement data recorded by the instrument via data communication. [Menu] -> [Results] -> [Day's Results] (Refer to "8.1 Day's results" in the MicrosEmi CRP User Manual.) You also could use the following operation. [Menu] -> [Results] -> [All Results] (Refer to "8.2 All results" in the MicrosEmi CRP User Manual.) Pressing [PRINT] on the measurement result screen will output the data via data communication. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. 30

3 Connecting to a network Outputting quality control measurement (QC) data via data communication You can use the following operation to output quality control measurement (QC) data recorded by the

37 3 Connecting to a network Outputting quality control measurement (QC) data via data communication You can use the following operation to output quality control measurement (QC) data recorded by the instrument via data communication. Example: Control N [Menu] -> [Quality Control] -> [Search Results] -> [Control N] -> (Refer to "9.4 Search results" in the MicrosEmi CRP User Manual.) Pressing [PRINT] while individual quality control measurement data is displayed will output the data via data communication. The procedure for control H and control L is the same as above. Tip Control N, H, and L are indicated as 1, 2, and 3 by the display format setting. Note Under instrument initial factory default settings, a measurement result is simultaneously output via data communication and printed when it appears on the measurement result screen. If you do not want results printed, use the procedure under " Printer settings" in the MicrosEmi CRP User Manual to change the [Printer Connection] setting to [OFF]. This will cause [PRINT] on the measurement result screen to change to [SEND]. 31

38 3 Connecting to a network Output format ASTM format ASTM frame format <STX> n Data <ETX> X X <CR> <LF> (1) (2) (3) (4) (5) (6) Definition Number of bytes Description (1) <STX> 1 Start (2) Frame number 1 Increments by 1 for each frame. Reverts to 0 after 7. (3) Data 240 maximum (4) <ETX> 1 End (5) Checksum 2 (6) <CR><LF> 2 - [Header], (Unused), [Order], [Result], [Comment], [Exit] A byte-wise add operation is performed an all of the data of one frame, starting from the character following <STX>, and up to and including <ETX>. The checksum value is the lower one byte of the result expressed as two ASCII characters that represent a hexadecimal number. General format of records (ASTM E-1394) A frame consists of records that are defined in accordance with ASTM E A record consists of ASTM fields. <STX>n...data...<CR><ETX>xx<CR><LF> Example of a record in a data record <STX>1H \^& HORIBA P E <CR><ETX>2A<CR><LF> ASTM record type Record ID H P O R C L ASTM definition Header Patient (Not used) Order Result Comment Exit 32

39 3 Connecting to a network Record configuration (measurement result data) H.. P.. C......O......C......C R C R C R C L Record field 1) Header Field Definition Description Format Maximum length 1 Record Type Record type H (fixed) 1 2 Delimiter definition Boundary code definition,,^,& (*1) 4 3 Not used Not used Sender Name Sender instrument name HORIBA 6 6 Not used Not used Not used Processing ID Process ID P (fixed) 1 13 ASTM Version Nb ASTM version number E (fixed) 9 14 Date and Time of message Measurement date and time YYYYMMDDHHMMSS 14 *1: Standard item " ": Field boundary; " ": Repeat boundary; "^": Structure element boundary; "&": Space boundary 33

40 3 Connecting to a network 2) Order Field Definition Description Format Maximum length 1 Record Type Record type O 1 2 Sequence Nb Sequence number 1, 2, Sample ID ID (specimen number) AUTO_SID Not used Universal Test ID Measurement type ^^^DIF 6 6 Not used Not used Not used Report Type Report type F 1 27 Not used Not used Not used ) Result Field Definition Description Format Maximum length 1 Record Type Record type R 1 2 Sequence Nb Sequence number 1, 2, Universal Test ID Measurement type ^^^Result name^loinc code (*2) 15 4 Data or Measurement Value Result value (*3) - 5 Unit or Set of units Unit (*3) 1 6 Not used Result Abnormal Flag Result flag (*4) 2 8 Not used Result Status Result status (*5) 1 10 Not used Not used Not used Not used Not used *2: LOINC stands for "Logical Observation Identifiers Names and Codes". 34

41 3 Connecting to a network *3: Output value format Unit Item Name STD INTER MMOL JPN WBC White Blood Cell 10e3uL 10e9L 10e9L 10e2uL RBC Red Blood Cell 10e6uL 10e12L 10e12L 10e4uL Hgb Hemoglobin g/dl g/l mmol/l g/dl Hct Hematocrit % L/L L/L % PLT Platelets 10e3uL 10e9L 10e9L 10e4uL MCV Mean Corpuscular Volume um3 fl fl um3 MCH Mean Corpuscular Volume pg pg fmol pg MCHC Mean Corpuscular Hemoglobin Concentration g/dl g/l mmol/l g/dl RDW Red Distribution Width % % % % MPV Mean Platelet Volume um3 fl fl um3 PCT Plateletcrit % 10e 2L/L 10e 2L/L % PDW Platelet Distribution Width % % % % %LYM Lymphocytes % % % % % %MON Monocytes % % % % % %GRA Granulocytes % % % % % #LYM Lymphocytes # 10e3uL 10e9L 10e9L 10e2uL #MON Monocytes # 10e3uL 10e9L 10e9L 10e2uL #GRA Granulocytes # 10e3uL 10e9L 10e9L 10e2uL CRP C Reactive Protein mg/dl mg/l mg/l mg/dl *4: L, H flags When a setting value is outside the preset management range, send is performed with an L or H flag stored in field 7 of the [Result] record. When the value is within the management range (no flag) or when a flag other than L or H is produced, send is performed with nothing stored in field 7. *5: Repeatability flags (*, $), CRP-related flags (E, F, X, C), and other flags (D, U, B, T) When the measurement result is abnormal or when data is suspected to be invalid (result is unreliable), the instrument will return one of the following flags in field 9 of the [Result] record. Note "W": No repeatability ($); "N": Result rejected (*); "F": Final result (none, L, H); "X": Invalid (D); "U": Invalid (U); "T": Temperature error; "B": Blank error; "e": CRP reagent empty (E) or CRP reagent sensor error; "f": CRP count acquisition failure, etc. (F); "x": Expired CRP reagent (X); "c": Incorrect CRP reagent (C) Only one measurement flag (the one with the highest priority) will be displayed and printed. 35

42 3 Connecting to a network 4) Comment ASTM specifications do not provide fields for pathology messages. The [Comment] record is used to send pathology messages. Different alarms are sent via [Comment] records for each alarm. Field Definition Description Format Maximum length 1 Record Type Record type C 1 2 Sequence Nb Sequence number 1, 2, Comment Source Model flag I 1 4 Comment Text Pathology flag (*6) Comment Type Comment type I 1 *6: Flag is sent as a single [Comment] record following the corresponding [Result] record. (See the example above.) Item WBC flag PLT flag Format L1,M2,G1,G2,G3 SCL,SCH,MIC 5) Exit Field Definition Description Format Maximum length 1 Record Type Record type L 1 2 Sequence Nb 3 Termination Code Sequence number Termination code 1 1 N 1 36

43 3 Connecting to a network Output example The following is an example of output when measurement results are sent from the instrument to the host. <STX>1H \^& HORIBA P E <CR><ETX>2A<CR><LF> <STX>2P 1<CR><ETX>3F<CR><LF> <STX>3O 1 AUTO_SID001 ^^^DIF F<CR><ETX>1B<CR><LF> <STX>4R 1 ^^^WBC^ e3uL F<CR><ETX>91<CR><LF> <STX>5C 1 I ^L1 I<CR><ETX>16<CR><LF> <STX>6R 2 ^^^LYM#^ e3uL L F<CR><ETX>10<CR><LF> <STX>7R 3 ^^^LYM%^ % L F<CR><ETX>97<CR><LF> <STX>0R 4 ^^^MON#^ e3uL F<CR><ETX>BD<CR><LF> <STX>1R 5 ^^^MON%^ % L F<CR><ETX>7B<CR><LF> <STX>2R 6 ^^^GRA#^ e3uL F<CR><ETX>19<CR><LF> <STX>3R 7 ^^^GRA%^ % H F<CR><ETX>12<CR><LF> <STX>4R 8 ^^^RBC^ e6uL F<CR><ETX>D5<CR><LF> <STX>5R 9 ^^^HGB^ g/dl F<CR><ETX>4E<CR><LF> <STX>6R 10 ^^^HCT^ % F<CR><ETX>9E<CR><LF> <STX>7R 11 ^^^MCV^ um3 L F<CR><ETX>B7<CR><LF> <STX>0R 12 ^^^MCH^ pg F<CR><ETX>14<CR><LF> <STX>1R 13 ^^^MCHC^ g/dl H F<CR><ETX>12<CR><LF> <STX>2R 14 ^^^RDW^ % F<CR><ETX>6F<CR><LF> <STX>3R 15 ^^^PLT^ e3uL F<CR><ETX>EB<CR><LF> <STX>4C 1 I ^SCH\^SCL I<CR><ETX>12<CR><LF> <STX>5R 16 ^^^MPV^ um3 L F<CR><ETX>91<CR><LF> <STX>6R 17 ^^^PCT^X-PCT % F<CR><ETX>0F<CR><LF> <STX>7R 18 ^^^PDW^X-PDW 10.5 % F<CR><ETX>E4<CR><LF> <STX>0R 19 ^^^CRP^ mg/dl H F<CR><ETX>4A<CR><LF> <STX>1L 1 N<CR><ETX>04<CR><LF> ABX format Same as the RS-232C output format. Refer to "2.5 Output format" (page 19). 37

45 2 Miyanohigashi, Kisshoin Minami-ku, Kyoto Japan

User Definable Settings

User Definable Settings Section 1: User Definable Print Format Settings...1 Section 2: Parameter Unit Selection...7 Section 3: Setup Menu 2...8 Section 4: Setup Menu 3...11 Section 1: User Definable Print