until now. NEW ZORBAX Eclipse Plus LC Columns No LC column has ever reliably produced such symmetrical peaks for basic compounds...

Transcription

1 No LC column has ever reliably produced such symmetrical peaks for basic compounds... until now. NEW ZORBAX Eclipse Plus LC Columns ZORBAX Eclipse Column Portfolio

2 I can t remember the last time I achieved ideal peak shape for basic compounds. Our lab is wasting too much time re-evaluating column chemistries and protocols. Lately it seems that trial and error are taking up all my time in the laboratory. Pyridine. Amitriptyline. Diphenhydramine. Resolving basic compounds like these requires both time and patience. That s because peak tailing is particularly pronounced with basic compounds, which tend to bind to un-protonated free silanol groups on the silica surface. To remedy this problem, Agilent has been developing a breakthrough column technology that quickly and efficiently eliminates peak tailing for bases. ZORBAX And now we ve perfected it with ZORBAX Eclipse Plus. 2

3 NEW ZORBAX Eclipse Plus LC columns designed to give you superior, reproducible separations of basic compounds. Eclipse Plus columns are a brand-new innovation that ensures excellent peak shape, resolution, and results for bases, acids, and neutrals. But what s really exciting is how Eclipse Plus columns outperform the competition. See for yourself Unsurpassed performance. Eclipse Plus columns stand up to even outperform the competition to help you Achieve excellent peak shape and resolution without the confusion of co-elution or hidden peaks. Significantly reduce tailing so you can confidently resolve difficult analytes. Perform ultra-fast, high-resolution, and analytical LC and achieve the right results under a range of temperatures, pressures, and ph levels. ECLIPSE Simple, reliable method development and transfer. Whether you specialize in pharmaceuticals, chemical analysis, foods, flavors, or environmental testing, Eclipse Plus columns can help you develop reliable HPLC methods under demanding deadlines. What s more, you can run, transfer, or replace existing HPLC methods from site to site without worrying about change or variability in your results. So the methods you create today won t have to be re-validated tomorrow. Eclipse Plus: Best Peak Shape in the Industry Without Tailing. 1. Pyridine (Base) 2. Phenol (Acid) Mobile Phase: 60/40 Water/ACN Ace C18 SunFire C18 XBridge C18 Discovery HS C18 Luna C18 (2) Gemini C18 XTerra MS C18 3

4 Other columns promise superior peak shape and efficiency for bases. Eclipse Plus delivers. Faster turnaround times. Unrelenting workloads. Fewer staff members. These are the challenges that every lab must face. And Eclipse Plus columns can help you meet these challenges by Enhancing efficiency and resolution with basic compounds, so you can better resolve and quantitate closely eluting peaks. Improving peak symmetry for your most challenging basic compounds as well as acids and neutrals. Quantitating and validating your results with a higher degree of confidence than ever before. Like all Eclipse columns, Eclipse Plus columns achieve their superior performance through extra-dense bonding, combined with a double-endcapping process. But Eclipse Plus columns also feature enhanced high-performance silica, new endcapping reagents, and a unique bonding process that is optimized for the improved silica. Peak Shape and Efficiency are Better with ZORBAX Eclipse Plus XBridge C18, 4.6 x 10 mm, µm N=14000 Tf= ZORBAX, 4.6 x 10 mm, µm N=23000 Tf=1.01 N=41000 Tf=1.13 N=60000 Tf=1.00 min What s more, Agilent s ZORBAX silica is manufactured by Agilent not purchased from outside suppliers. And that means we control every step of the manufacturing process, ensuring lot-to-lot consistency and long-term, reliable results Gradient: 10 to 30% B/1 min. A: 0.1% FA, B: 0.1% FA in ACN Flow: 1 ml/min. Temperature 40 C Elution order: sulfanilaminde, sulfadiazine, sulfathathiazole, sulfamerazine, sulfamethazine, sulfamethoxazole min ZORBAX Eclipse Plus: Lowest Tailing Factor with Amitriptyline in Methanol Mobile Phases Tf = 1.2 Tf = 1.00 Gemini C18 Tf = 1.26 Luna C18 (2) Tf = 1.20 SunFire C18 Tf = 1.92 Ace C18 (2) Inertsil ODS (3) Tf = 1.11 Amitriptyline ~ 0.1µg, 80% Methanol: 8mM total Potassium Phosphate Buffer ph 7.0, 21 nm, 1.0 ml/min, 4.6x100 mm, µm columns 4

5 The proof is in the performance! Day to day column to column Eclipse Plus is the name you can trust for consistent chromatography. Column performance and stability are key to producing results that stand up to scrutiny. And Eclipse Plus columns surpass the competition on both counts by Combining excellent peak shape with the high efficiency of a silica-based column. Performing well under difficult testing conditions, such as Acetontrile mobile phases. Continuing the ZORBAX tradition of long lifetime products for superior reproducibility. The following graphs prove how Eclipse Plus consistently delivers the lowest peak tailing factors even with complex pharmacological analytes. Peak Tailing For Basic Compounds is Lowest with Eclipse Plus SunFire C18 USP Tf of Diphenhydramine/Amitriptyline-ACN The only column with Tf=1.0 in ACN Gemini C18 Xterra MS-C18 Luna C18 (2) Tf Diphenhydramine Tf Amitriptyline Ace C18 Discovery C18 Tailing Factor Eclipse Plus Delivers the Best Performance with All Samples N=10747 Tf=1.03 N=10968 Tf= Luna C18 (2) N=9307 Tf=1.09 N=9377 Tf= XBridge C18 N=898 Tf=1.14 N=8737 Tf= min Columns: 4.6 x10 mm, µm Mobile Phase: 60:40 Water: MeOH Flow Rate: 1 ml/min Temperature: 40 C UV 24 nm Sample: Corticosteroids: 1. Triamcinolone 2. Prednisone 3. Hydrocortisone 4. Cortisone

6 Flexibility, scalability, and convenience to accommodate virtually any application. Eclipse Plus columns are available in, 3., and µm particle sizes for all your analytical, high-resolution, and fast LC analyses. From nano to analytical to prep Eclipse columns allow you to scale from µm to 7µm and to transfer methods anywhere in the world without worrying about reproducibility. So you can spend more time running samples, performing analyses, and meeting deadlines and less time re-evaluating new column chemistries or revalidating protocols. Method Scaling with Eclipse Plus: Reduce Column Length and Particle Size For Fast Analysis Without Sacrificing Resolution 4.6 X 20 mm, µm X 10 mm, 3.µm min Need to run twice as many samples in half the time? Choose Eclipse Plus Rapid Resolution High-Throughput columns with a µm particle size. They re specifically engineered for ultra-fast separations and superior method development flexibility. So you can enhance your lab s productivity without compromising reliability X 0 mm, µm A=pH 4. Na Acetate, B= Methanol (60:40), 1.0mL/min, Flow cell: Micro/High Pressure 6mm, 1.7µL Detection: UV 24 nm With Eclipse Plus µm LC Columns, You Can Perform Fast and Ultra-Fast Analysis of Complex Compounds Like Berberine min O N+ Clmin 4.6 X 0 mm µ Less than 1 minute analysis!! Perfect peak shape! O OCH X 10 mm µ Tf= 1.00 ZORBAX OCH 3 Berberine Structure Columns: As listed Mobile Phase: 0%ACN: 0% 8mM K2HPO4 ph7, Flow Rate: 1.0mL/min, Sample: Berberine 0.4mg/mL, 2.0 µl injected Detection: UV 24nm Tf= 1.06 min 6

7 No matter how many samples you have, or how complex they may be you need to feel confident that you can achieve reproducible results without wasting valuable time testing different columns and configurations. And that s why Eclipse Plus columns are such a significant breakthrough. Simply put, Eclipse Plus columns deliver the sharpest peak shapes for basic compounds from the very first injection. And they continue to deliver the long-term reliability that is so critical for methods that last 1 years or more. The following table will help you select the column dimension, ID, and bonded phase that will deliver the best results for your application. For the latest product specifications, chromatogram libraries, methods, and other resources, visit Eclipse Plus Ordering Information Column Description Semi-Prep Analytical Analytical Analytical Analytical Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution HT Rapid Resolution HT Rapid Resolution HT Solvent Saver Solvent Saver Plus Solvent Saver Plus Solvent Saver HT Solvent Saver HT Narrow Bore Narrow Bore RR Narrow Bore RR Narrow Bore RR Narrow Bore RR Narrow Bore RR Narrow Bore RRHT Narrow Bore RRHT Narrow Bore RRHT Guard Cartridges, 4/pk Guard Cartridges, 4/pk Guard Hardware Kit ECLIPSE Size (mm) 9.4 x x x x x x x x x x x x x x x x x x x x x x x x x x x x x 12. Particle Size (µm) Eclipse Plus C18 USP L Part numbers shown in black are available April 01, Part numbers in red will be available August 01, Custom columns are also available. Contact your local Agilent Representative or Agilent Authorized Distributor. Eclipse Plus C8 USP L

8 Eliminate the frustration of failed separations with Agilent s complete ZORBAX Eclipse LC column portfolio. Eclipse LC columns are the only scalable column family that lets you Minimize tailing and maximize peak symmetry for bases, acids and neutrals. Develop and transfer methods without re-validation. Deliver reproducible results across a wide ph range and under varied separation conditions. Rapidly scale from analytical to prep or from conventional to ultra-fast methods with particle sizes from µm to 7µm. Process samples up to 20 times faster and improve resolution up to 60% with high pressure ZORBAX Rapid Resolution High-Throughput columns (maximum pressure: 600 bar). Additionally, Eclipse LC columns feature double-endcapping, and a unique bonding process. This ensures lot-to-lot consistency, extends column life and allows you to retain your methods for the long run. ZORBAX Eclipse LC columns are the first choice to start any separation. Here s why. Eclipse XDB Columns Provide outstanding separations over a broad ph range (ph 2 to ph 9). Perform reliably under high pressures and flows a result of Agilent s proprietary ZORBAX hard-walled silica particles. Offer method development flexibility with broad selectivity choices of four bonded phases and a wide range of particle sizes and dimensions. No wonder Eclipse XDB columns are an analytical and regulatory standard across many diverse industries such as pharmaceutical, environmental, chemical, food, and nutraceutical. Every ZORBAX Column Undergoes Rigorous Quality Assurance Testing and We Prove it In Writing. ZORBAX 8

9 Tired of choosing between speed, resolution, and sensitivity? Choose ZORBAX Rapid Resolution High Throughput (RRHT) columns. ECLIPSE From complex protein digests to ultra-fast separations Agilent s RRHT LC columns deliver the right results on time. In your lab... or any lab throughout the world. ZORBAX RRHT LC columns are specifically designed for high-speed and highthroughput applications, allowing you to Process samples up to 20 times faster than conventional HPLC, while achieving equal or greater sensitivity and reproducibility. Reduce your analysis time by up to 9% without compromising resolution. Easily scale and reliably transfer conventional LC methods without re-validation. Analyze complex separations on shorter columns. Identify highly sensitive compounds through LC/MS with less co-elution. Enhanced flexibility: ZORBAX RRHT LC columns are available in almost 100 configurations including: Lengths from 1mm 10mm IDs from 1.0mm 4.6mm 8 ZORBAX bonded phases, including StableBond, Eclipse XDB, and Eclipse Plus µm columns for high-pressure use (maximum pressure: 600 bar) Run samples up to 20x faster than conventional LC with Agilent s 1200 Series Rapid Resolution LC System. Features include... 60% more resolving power than traditional LC A peak capacity greater than second cycle time Efficiencies above 60,000 plates The ability to run your current methods Comprehensive support on the Web, by phone or in person to ensure top performance Together with Agilent s ZORBAX RRHT µm LC columns, the Agilent 1200 Series Rapid Resolution LC System can help you produce your numbers more efficiently than ever. 9

10 From particle size to ID and length We offer an unsurpassed variety of columns to accommodate virtually any application. ZORBAX RRHT µm LC columns for high-pressure use (maximum pressure: 600 bar, 9000 psi) Best for fast and high-resolution LC. Eclipse XDB-C18 Eclipse XDB-C8 SB-C18 SB-C8 SB-CN Extend-C x 10 mm 4.6 x 100 mm 4.6 x 0 mm 4.6 x 30 mm 4.6 x 20 mm 3.0 x 10 mm 3.0 x 100 mm 3.0 x 0 mm 3.0 x 30 mm 3.0 x 20 mm 2.1 x 10 mm 2.1 x 100 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 20 mm 1.0 x 100 mm 1.0 x 0 mm ZORBAX RRHT µm columns and cartridges (maximum pressure: 400 bar, 6000 psi) Best for fast LC. Eclipse XDB-C18 Eclipse XDB-C8 SB-C18 SB-C8 Extend-C18 Standard Columns 4.6 x 0 mm 2.1 x 0 mm Cartridge Columns Require hardware kit x 0 mm 4.6 x 30 mm 4.6 x 1 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 1 mm packs are also available. Only ZORBAX Eclipse LC columns combine choice and flexibility with Proven performance and reproducibility through millions of injections Extra-dense bonding and double-endcapping Superior scalability Excellent performance for all sample types Results you can trust across a wide range of separation conditions Extensive Applications Library for faster method development Worldwide support, worldwide supply Other bonded phases are also available. Visit our online store for details: 10

11 ZORBAX Eclipse Rapid Resolution 3.µm HPLC columns Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-CN Eclipse XDB-Phenyl 4.6 x 10 mm 4.6 x 100 mm 4.6 x 7 mm 4.6 x 0 mm 4.6 x 30 mm 4.6 x 20 mm 3.0 x 10 mm 3.0 x 100 mm 2.1 x 10 mm 2.1 x 100 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 20 mm 1.0 x 10 mm 1.0 x 0 mm 1.0 x 30 mm Capillary Glass-lined Columns 0. x 10 mm 0. x 3 mm 0.3 x 10 mm Cartridge Columns 4.6 x 7 mm 3.0 x 7 mm Cartridge Holder Guard Cartridges 4.6 x 12. mm, 4pk Guard Cartridges 2.1 x 12. mm, 4pk Guard Hardware Kit for 4.6 & 2.1 mm Guard Cartridges 1.0 x 17 mm, 3pk Custom column available. Contact your local Agilent Representative or Agilent Authorized Distributor ZORBAX Eclipse µm HPLC columns 21.2 x 10 mm 21.2 x 100 mm 21.2 x 0 mm 9.4 x 20 mm 4.6 x 20 mm 4.6 x 10 mm 4.6 x 0 mm 3.0 x 20 mm 3.0 x 10 mm 2.1 x 10 mm 2.1 x 10 mm Capillary Glass-lined Columns 0. x 20 mm 0. x 10 mm 0. x 3 mm 0.3 x 20 mm 0.3 x 10 mm 0.3 x 3 mm Cartridge Columns 4.6 x 20 mm 4.6 x 10 mm Cartridge Holder Guard Cartridges 17 x 7. mm Guard Cartridges 4.6 x 12. mm, 4pk Guard Cartridges 4.0 x 4 mm, 10pk Guard Cartridges 2.1 x 12. mm, 4pk Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-CN Eclipse XDB-Phenyl ZORBAX Eclipse 7µm HPLC columns 21.2 x 20 mm 21.2 x 10 mm Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-CN Eclipse XDB-Phenyl

12 When you choose ZORBAX Eclipse LC columns, you get more than just a dependable product. You also get 40 years of chromatography expertise from the world s largest chromatography supplier. So you can avoid problems and more effectively address any challenges your lab might face. For more information about Agilent s ZORBAX Eclipse Plus and Eclipse LC column portfolios, visit us online at Call toll free: , option 1 (in the US and Canada) Or contact your local Agilent Representative or Agilent Authorized Distributor. For technical assistance in selecting the column that provides reliable, reproducible results on time, every time call , option 4 (in the US and Canada). For information about the Agilent 1200 Series Rapid Resolution LC System, visit us online at Don t forget to visit Agilent s Online Store at for easy ordering and up-to-the-minute information. Count on Agilent for world-class technical support on the Web, by phone, or in person. Information, descriptions, and specifications in this publication are subject to change without notice. All rights reserved. Reproduction, adoption or translation without prior written permission is prohibited, except as allowed under copyright laws. Agilent Technologies, Inc Printed in the USA April 20, EN