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1 :!"# $%&' (Tagetes Minuta) ()* +, * *? Ph.D.! M.c. M.c. * ** M.c. #$ M.c. " &% 4?G H$ E56F#@,3'3: :2 B ' BCD ',3'3: 3A, ; :'.IJ:& (<6;,?) <3:?6L. 62 M', 9' 6 J, #, NO P5 M', :) *+ 4?G H$ BWX J, E NO Y>X B>1.3 U6V7 T+L ', 67.3'2 'J, :@ Z13A,, &A, %9 6 IJ:& 4?G H$ E56F#@,3'3: :2 B Z13A NO, <9: ['>: :, E56F#@,3'3: IJ:& 4?G :2 3 J, 23:, J %P] '$.%;, \ IJ:& M', ['>:.3 >6# 6 H IJ:& H$ :2 >6# 6 H26 34/5.,, W89'J@ ', E56F#,,3'3: 3 $#.J, 62 M', 35 <6 C#_ M'>96 ' BCD W62 NO AG `6$7 3@ %; ['>: 4;, :.,/ IJ:& 4?G BW# ',?'$ IJ:& H$ BW# ', 6O, Z13A 6a7 5 G E'J'3'3: < IJ, F67 b' Z13A BW, J, >?2 >;,., <9: ex>:, J, ff1 Z13A d;5 B>?7 J, >;, M', J, c%;, 3,3'3: BW:2 %$ B>$ gw 5, M', %;, BA 3 h B>:O J, i' W B, d;5 B, B, 3@ %; ['>: D7.2 d;5 : BWf7 ex>:, ': I>5 \ Z13A kf5 <,5O NO M', E56F#@,3'3: BW# ', 3 B j& NO. E'J'3'3:.Z13A %6#C& IJ:& E56F#@,3'3: ' BCD : /4/6 :#$.'/ 86/4/1 :#$ *+, 85/5/21 :#$ %&' <,', <,7 43 %67 89:, 39:, ; , 45? 43 %67 89:, 39:, ;501 2 * 43 %67 89:, 39:, ;54' 2 ** azadeh_myco@yahoo.com :6:>#, %? 4@
2 <,W BW# ', 6O ' BCD #:7, NO ',3'3: 3A %6#C& C#_ :2 Z.35>?W %'w f1 <D ;,; #; i' T. lunulata, T. erecta, T. patula, T. `n5 g6 #; J, :2 Z M',.3 <@ 9 tenuifolia W62 BW, <,5O 3 ' e] BJ9 M] B >X C#_.3:62,1 >;,, <9: C#_ M', %;, 3 H:, Tagetes BW:2 F67 36#7 '66 F67 M'> <,5O Ocimene Tagetes BW:2.35>?W > ow 5] xf'$7 %;, T. minuta J, 3@ %; M] T. M', 6: f, <@ F67 J, 3, F67.%;, D % <, 6 tenufolia y %;, 3@ %; T. patula J, W62 M] <,5O <3 E'J'3'3:.(14) 3 piperitone limonene dn% & B'6 M'7k' M'> J, ' i.,,1 WX >; <@ 355 ', fo %;, <?:, ML: %; M G %7 G %:O W2 3'.,2 8>; ' g: <m, yx >&2 6:, 6] d1 3F 6 2 9>5 6: %:O fo M6Z <5, ',3'3: BW:2.J; 9.(5) 3: e? :>;6 9>5 BW, %F?: %;, M', *_ ',3'3: f,o +>F,&, < x+o E.3:,3 H$ #J, <, 6 g', &, 8' f'? J,.(6) %;, 6a7 E'J'3'3: ', E'J'3'3: ;7 %;, :>;6 B>:O f,o <3 M' 8'D ;7 >96 >:O M', J, 'W:2 %$ y 3>&, j7, 3'3D ',3'3:.(7) %;, #J@ %F?: B,J E6#67 7,+2 f6f1 Z, '66 BW, J, >;, E'J'3'3: < BW, M6$$ D7 J, E (8) 35 f9 <W62 g$: %6W, D7.%;, 3 dd W62 >5; dn 5] >/2 B,, <,', M', '6 < B3O? 56J 3 H ;7 : 62 %6#F$ %;, W62 (Asteraceae) Tagetes minuta &&2 O H: ' BCD >; f2,:l `C> #; i' B'@ :>?W, O G,: 62 M',.3 l2, 6 M6>:,m@ 6P: 'W9 f 5D Y>X `n5 62 M', 3 f'7 f6#.%;, B6# 3W9 6: <2:2 G,: <@ J, B6? BW:2 F67 f6f1 J, ': F67 J, 5] Tagetes minuta.(1) W36l:+& WM7: B,$G B,$G i7 B$G6] %;,, <9: 3W,.3 i67@ F67 5&67 B,3:,J,, Tagetes D ': F67 M', <m7 BZ1 Z1 35: Y>X BWo?6:2, B &@ W7: W77 B$G B '> :?:, M',.35,, Y>X BWo?6: `'n J, B,9G 3 3W9.35>?W 6: :?:, ',.J, B,, F67 Tagetes minuta (Z)ocimenone, tageteone, (E) D ': d67 [5 %;, ocimenone B: 6 3A %6#C& B,, Dehydrotagetone, Bocimene '> B 62 M', : W.3 3 B,, :@ F67 3'2 qx9 3,, Z1, %95 %Fr H2 B'> B 23:, J, 3 23:,J, %100 5 H2 B'> B 23:,J F I; Hudson.(2) 35 Z1 B C#_ ;' 3A,, 62 M', <2:2 ': ;' 3A, B,, M6l J 62 M', : % '## WM&67 3 3W9 M65tW %;, 'u %6#C& M'>96 B,, 35>?W M&67 gx 2 J, g6 B,, I; Chand Hoke & Ghatak.(3) 35 ;' 3A qx9 3:, H:, 7 :,6G B 7C#_ 1969 <L 9& gw vo Tagetes minuta M] 3:.(3) %;, M? %6 L B,, e>#, 3A 7+': <W62 M', 7 %;, :2 56 f Tagetes E5D 22
3 I656@ H <3 ~.o' &A, 6 3 fg q#l f#, ;; Ä5', 3:15 9& %&2,1 +7, 6 E{;.3'2 f'>;, $ c B k %65'm, +>F,&, J, 3,3D BG 2@ J>? ; %9 6 B ',3'3: B,38: 2448h 3 37 c B Å6$7 I656@ N6X97 B>?7 J, BX BW5 3W9 J, E.3 >;, W# ', '; J, E56F#@,3'3: i67 B, 1,>&,.3'2 673 / C ## X 5 J, Ç# i'. 33 3; # Å6$7 2 M65D H; ' <?:, H; J, >6# 6 0/5 B> J, E{;.3 B,38: 37 c B %O; 2/53 3.%&2,1 ; ',J ## 36#7 P: 2@ f6 < 6.D+. #.o': ;, {;36+ ', 80 M6l7 %0/5 673 / E; 67 2@ J>?; %9 6,3>,.F+/1 ) f'>;, Ç@; ;7 W# ', E{;.3 >X' W%6 $ E{;.3, %9 6 M', B f'>;, E5 ;7 IJ:& BWi?' 7:, %O; %&2,1 %6 ; %l,1 NCCL 3> 4;, H,26 >:@ ['>:.3 B,38:.3 67 %D.4 $E< 13 GH A; M',.3 >;, (<6;,?) <36:?6L. J, NO :@ ÉL J, W7 9',J G P5 %D G 35Z %1 W9'.3'2,3D 62 1; J, J, H2 150 <, 6 E{;.3:3 >? 62#@ < ~n >6# 6 500,3$ 3 >X' M#, < e6;@ W9' %O; '2 &A, <@ D 70 I:7,, ~} M'35Z 3 M', n) 3,,1 89'J@ B.(8) D <. M', O C;7 %D <,, M'> B+6 19 <1 ;,, 7 : 62 \, BG 'W, J, >;, ['37 # %;, < J, 6 M', M69:D 3:, ', <W62 D,& B3G 7 ', <W62 d677 M',.3'2 J, >?1 ' H7 '66,, L BD 3 {; e_:,o <3 W} %O,., O5N :@ <?:, %C6Fn <@ B2J; H3O i6>>5; F67 F:D ~_C : 62 <,89Wb <,359:, D7 8' h BWA D7 >;, M6W.(9) %;, 3'2 BCD NO Z13A, ; ~3W AG `6$7 3 <,', E56F#@,3'3: BW# ', B '.%;, >&2 &) *+ ',3'3: BW# ',. $, 3+4 i'.%;, 3 67 : 35Z J, C#_ M', >;, <,5O E56F#@,3'3: PTCC &6# :: <,', >C5 O BWgWb <J; J,,3:>;, ::.3'2 67 / / 673 / 89:; + < %65'J, +>F,&, J, B62:: H:, J, E.3) '; J, E56F#@,3'3: BW# ', :@ B@kD ',3'3:.3'2 >;, f'h BW. J, P5 M'3.3:3,3D W# ', :: W J, B,3$.>+?2, ) ~ %15 4> J, >;, %&2,1 67 Hu i' B BWI; f Z1 5O BG 'W::.3 <3 ',W >6 35 _1 6 2 BX ';5 BJ;,3D %9 B, 3: H6?6 :,D.35>&2,1 >;,,3'3: BW:2./ A; J>?; 6 J, H2 65.@: B! A; <3:D J, E fg _$ e@ >6# i', 2@ 23
4 <,W BW# ', 6O ' BCD #:7, NO ',3'3: 3A %6#C& C#_ J, WX %99/9 >P] M'>.3, %9 HJu.(11) 3'2 e? MFC <,5O 3: >& M6 n F7 3 f1,3g 9'J@ J, H,3 W %;, h.3 F; :@ ['>: M68:6,7 :2,3D N" 2 ) C TH 3 " E; Minimal Minimal ) C : + (Concentration J, g'j@ M', H:, B,.(Fungicidal Concentration BW# ', J,,3>,.3 >;, %4 2@ J>?; 6 J>?; 6 B f'>;, Ç@; ;7 P: >6#6 50 og 'WiWZ E{;.3, %9 2@ #, NO J, >;, 3'2 6FC7 %9 6 B NO J, Y>X '>1 f'>;, Ö, J>?; 6.3 &A, iwz 67 >6#6 40 og #, #:7,36W I+G J, %?7 BWiWZ 5 M65tW <6;:, %O; 2448 J, E.3'2 >;, 3W <,5O `61 n 3 H3O BW#W _1 37 c B >6.3 >&2 J,3:, &&, 6 J, o?6:2, 6#, BJ;,3D >;, (B> 3A i676 >:@+2@ J>?;) CC 37 c B B,38: %O; 2448 J, E 3.3: 3 %9 6 B BX BW5 %F?: ',3'3: BW'; %6;?G M66C7 J, f G ['>: ~,n, 3 G,: _1 B62 J,3:, 4;, IJ:& 3 qx9 IJ:& BWi?' BW# ', NCCL 3> 3 M66C7 BW,3:>;, 4;, H3O #W _1 'W# ',.3:3 ';5 H$ 4?G _1 'W# ', H$ > 6 12 J, > :@ 3 BW# ', > 6 24/5 J, >96 <3 H3O #W g'j@ M', ['>:.3:3 B35$Fn, %F?: 4?G.%;, 3@ 1 I3D < '> / 3 J, 3C c(o'j ow, <7 6_$7. I+G E{;.3'2 ~ & /] M#,.3'2,3D NO J, D 40 B ÑL <J M66C7 P5.13 GH I:J K+ E; <, 3 ~ I J, >6# 6 5 <, 6 NO i9l 3 HC 9O, o1 ; <@ <J %O; 96 7 %&2,1 D 105 B %O; 3 I M',.o6>X' %O; 96 x,3 <3 i9l J, E 3'2 i9l x+ <,5O #L %O; 96 <J <@ <J ~+>L, 3 <J.3 >&2 P: i9l NO <J Minimal Inhibitory ) A A,L1M N" E; Minimal Fungicidal ) : + (Concentration ) 4 $E< 13 GH (Concentration J, P5 M', B,. B! Q" *+.3 >;, B,:L 96 BW%66 J>?; 6 >6#6 100,3$ iwz W,3>, I, iwz NO >6#6 100 E{; >X' Ö, 100,3$ NO 6 <3 yx J, E.3 &A, J, E 3 H iwz I, iwz J, >6#6 3 &A, H; iwz >6#6 100 :@ <3 yx 100 L@ iwz J, x>': %&',, L@ 7 d677 M6W 100 iwz W 3C G.3 >X' <6 >6#6 f %Fr 3W Y' W.3'2 &, BX I; <56n, %D) f'>;, Bm#' 6& H; X %9 6 (<@ 3 B, 6 < B/V o?6:2, < 3:J J, M',.%&2,1 f#, %9 6 f 5 3W i' H iwz H i' NO %1 I, iwz d677 iwz W %,,1 o>9w i' %1 H; iwz HZ 3 %66 E{;. BX I; 1000 BG WZ M6#,.%&2,1 37 c B %O; 2448 P: <,5O %&2 5 3 <@ J, E WiWZ W '> J, 6: MFC M66C7 B,.3 >&2 2@ J>?; %9 6 B >, B,3$ 24
5 L/ 3+4 )27V.1 F+< T T Q P O N M L K J I H G F E D C B A (>6# 6 24/5 J, >96 #W _1) 4?G : (PTCC 5027),3:>;, E56F#@,3'3: :T (12 mm J, > #W _1) H$ : D=0.05 mg/ml B! Q" *+ ) 4 $E< 13 GH MFC + Y,/ Z/.2 F+< MFC A (mg/ml) 1/ /64 69 A,B,D,E,J,I,G,K,N,O,,TT 1/128 34/5 1/256 17/25 C,F 1/ /128 34/5 H,L,M,P,Q, $E< 13 GH A ][ E; Y,/ %6 g'j@ M',.K0$ *+ ) 4 NO 69mg/ml %P] 2@ J>?; 6 BG A J B E D I G K N O T T BW# ', #, +) B! + ) 3+4 C TH3 ".3 F+< )) 13 GH ) ]+. +, 3/; + F,3 + 40?. (, 1 _. $, C KH ) NO 34/5mg/ml %6.35>,3: 3 %P] BG %6.3: : 3 Q P M H L BW# ', #, C 13 1/32 22/1 ± 1 1/64 19/3 ± 1 1/128 1/ A 3 F C BW# ', 3 17/25mg/ml %P] BG.(2 I3D) <J M68:6.13 GH I:J K+ E; Y,/ 19/1 ± 1 24/6 ± 1 25 ± 1 16/7 ± 1 22/3 ± 1 20/3 ± 1 17/3 ± 1 17/8 ± 1 B C D E. >6# 6 H2 6 7/ 73 ± 0/15 #, NO i9l BCD #, NO MFC M66C7 J, f G ['>: 24/1 ± 1 25 ± 1 24/2 ± 1 21/9 ± 1 23/2 ± 1 22/3 ± 1 19/1 ± 1 20/2 ± 1 18/1 ± 1 F G H 1/64 1/32 B>1 Ö, M', 6. ' IC B,, W# ', %28 :,1 M', J,... 1/128 25/1 ± 1 25/2 ± 1 20/5 ± 1 22/2 ± 1 I J K %9/2 69mg/ml BW# ', <6.3: IC IC B,, %62/8 17/25mg/ml IC 34/5mg/ml B,, B,, %11/48 IJ:& %F?: 4?G 26 ± 1 25 ± 1 24/7 ± 1 20/3 ± 1 22/1 ± 1 24/8 ± 1 21/6 ± 1 19/3 ± 1 L M N O 34/5mg/ml <6.3: IC 69mg/ml B,, %32 IC 17/25mg/ml B,, %56/52 26 ± 1 22/3 ± 1 25/2 ± 1 22/1 ± 1 20/4 ± 1 23/3 ± 1 22/6 ± 1 19/6 ± 1 P Q %14/28 B,, %57/14 34/5mg/ml IJ:& H$ BW# ', B,, %28/69 69mg/ml 26 ± 1 22/3 ± 1 21/6 ± 1 19/1 ± 1 19/7 ± 1 11/4 ± 1 19/9 ± 1 T T.(3I3D) (21,:) 3: 17/25mg/ml IC B,, ()=Inhibition Zone not seen 25
6 <,W BW# ', 6O ' BCD #:7, NO ',3'3: 3A %6#C& C#_ à,:, J, >;, >;, M',.(10) %;, 3., 2 >:O B>:O < u '$ b' Z13A BW, E56F#@,3'3: J, : B>:O b' i6>?6;.%;,,w / BW, %F?: %$ ', B, 35>?W 9 e6 F67 WIJ,36', %6 L IJ@ $>9.3: <@ â vo o?6:2, B9] O, M65Z <,7 Bn.3:, ; 6 3A B 5 H2 B'> B5r>;, W, M', : 7F67 WIJ@., 6a7 :?:, B, '6 B'> 6$ p450 H>6; o>?6; BWo' :@ d67, 35>?W >L; I>;2, >5; I+>L, dd Z1 B#; Z1 BW I; â >': #;,' q1: <3.(11) 3: p450 H>6; ' :@ o>?6; F67 M', 'D <@ J, ',L ~N 35W <9: g5, 6: <,,3:>? un >5; B3C> u+>l, dd :@ $' 7,W Y>X,O %': >92 B36 6#> ~N J, 3C :@ >5; B, D J,. 35W,L J, <,7 f>x WIJ@ $' 7 ',L 36;, i6l567 :?F7 WM'7# <Z '6763:,.(11) H: :m3:@ 36l67#,56 W36l672 B>:O %;,, <9: i'm#636, C#_ ', Z13A BW, H$ BW:2 ;7 Z1 o,3'3: BW:2 änl àa M',.,1 36'a7 IJ:& BW, %F?: :@ %$ ['>: %;, 3 qx9 _, M',.(12) %;, >&2 01 B62b' 6P: >X f,o Z13A < i' < 6 BW>& Z13A B, B62b' %:O fo.(13) 3 >?, BW,, %$ fr,o 01 B62b' lg 01 G <, 6 B6 fo 01 à: Z13A Y>X BWF5D J, Z13A BW, M65tW.%;, %6W, <,, ', fl,37 o?6#> k'j7 e/d %61 6P: + 2 ) C TH 3 " E; Y,/ Y>X B>P] 6: g'j@ M',.13 GH MFC M66C7 3 H3O #W _1 3 &A, WiWZ #, NO.3'2 60% 56.52% 50% 40% 32% 30% 20% 11.48% 10% 0% =34.5mg/ml =69.5mg/ml = mg/ml ) g 3+4 ) 13 GH f.1 /.F+/ % 57.14% 50.00% 40.00% 28.69% 30.00% 20.00% 14.28% 10.00% 0.00% =34.5 =69 =17.25 ) T ) 13 GH f.2 /.F+/1 h&&!) 35>?W 6 B>:O J, W2 Z1 B>:O q'$: +>F B: 6,3C7 g', &, f6#, r,.35w á 5', o>?6; à,:, J3', 6P: Y>X f'u 6L, W 676>:@ ' ~N :L BW6L3 J, >; M', g', &, 5F B'J, W36l>;,67 26
7 eferences.3,l E56F#@,3'3: 1. Kumzr A, Dnkel F, Matthew J. Broughton. Effect of root extracts of Mexican marigold Tagetes minuta on six non tatget aquatic macroinvertebrates. Physiological and chemical ecology 2000; 29(2): EL Deeb K, Abbas FA, EL Fishaway AE, Mossa J. Chemical composition of the essential oil of T. minuta growing in audi Arabia. audi pharmaceutical journal 2004; 12(1): Cestari IM, arti J, Waib CM. Evaluation of the potential insecticide activity of Tagets minuta essential oils against the head Lice pediculus humanus capitis. Neotropical Entomology Camm EL, Twoers GHN, Mitchell JC. UV mediated antibiotic activity of some composition species. Photochemistry; 14: Calderon A, Foniz WA. Virulence factor of Candida albicans. TE Micro ev 2001; Elie CM, Lott TJ, asse E. apid identification of Candida species with speciesspecific DNA probes. JCM 1998; 36(11): Ahmad, Kan Z, Mustafa A. eminested PC for diagnosis of candidemia: American society for microbiology. JCM 2002; 40(7): B ã Yã>X NãO 6 'm#6 %6#C& ;.4 <J %Cn cbj;, 39:,.<,7 HO 89:,.>6&7.7ä :1368.B36; 6 D7.?:' H6., H6:@.9 ã 6ã; NO J, 3,3D g5,,. '_#, *H 6WJ à : :1(2) c1375.w89'j@. 3:6.I, ÇãZ.kãD ã ;5 01.H, à4, 3F ~ 5'J c<,7 89:, ÇZ,9>:,?; :<,7 \ J <, ' Y1>,, ', b' j& f'u.35>?w 56# %6W, B,, %; M6$$ D7 6L, B#; ', B>$ ä,l W62 C6Fn F67 M>&' 6a7 ; AG `6$7 ~3W.%;, 3'2 ~_C Z1 X BW# ', 3 ' BCD #, NO B 3, <9: AG C#_.3 E56F#@,3'3: , B,, ' BCD #, NO E56F#@,3'3: IJ:& H$ 4?G BWM'>;, BCD #, NO 6a7 ; J, 3@ %; ['>:.35>?W ; %7 E56F#@,3'3: Y>X BW# ', ' 1 %P] >?, `'n J, NO M',, <9: 20 à J,.3 E56F#@,3'3: BW# ', L 3 8 4?G IJ:& %F?: # ', 12 ; # ', BW# ', M6 NO M', 3 3: H$ # ', >6# 6 H26 17/2569 IJ:& %F?: 4?G IJ:& H$ BW# ', M6 3 H J, 7u B>P].%;, >6# 6 H2 6 ; BW# ', 3 f vo >6# 6. E56F#@,3'3: 7u, MFC '$ ' BCD #, NO 3 dd I:& 4?G BW# ',?'$.3'2 E56F#@,3'3: IJ:& H$ BW# ', %$ M6 35W <9: ['>: M',..,/ %F?: <@ %$ IJ:& %F?: E56F#@,3'3:,1 6$>? yf7, ' BCD 62 Z13A,, / 62, D I>G, M', M',5.%;, 3 dd IJ:& B, 9?6: B>96 $6$7 J6:, M', F,. E56F#@,3'3:, <9: `6$7 M', J, 3@ %; ['>: à., BW, 35:W 3:,7 ' BCD W62 NO 3 'u BW%61 J, i6>>5; Z13A 27
8 <,W BW# ', 6O ' BCD #:7, NO ',3'3: 3A %6#C& C#_ 12. Canoto MM, odero FG. Antifungal drug resistance to azole and polyense. Lancet Infect Dis 2002; 2: ã : J{6# o' :@ B62b' J6 NO 6å7 ;.m :J.13 >ã 3ã, ã;5 ã: <'.W89'J@ ', && ' ;u.1380 c43 %67 89:,. ;
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