LV-DAF Dynamic Auto Focus Instructions

Transcription

1 M462 E 08.1.NF.1 (2/3) LV-DAF Dynamic Auto Focus Instructions

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3 Thank you for purchasing the Nikon products. This instruction manual is written for the users of the Nikon LV-DAF Dynamic Auto Focus. To ensure correct usage, read this manual carefully before operating the product. No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon. The contents of this manual are subject to change without notice. Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative. Some of the equipment described in this manual may not be included in the set you have purchased. If you intend to use any other equipment with this product, read the manual for that equipment too. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Microsoft, Windows, and Internet Explorer are registered trademarks of Microsoft Corporation in the U.S. and other countries. Other product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners. 1

4 WARNING and CAUTION Symbols Used in This Manual Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference. Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols. Symbol WARNING CAUTION Description Disregarding instructions accompanying this symbol may lead to serious injury or death. Disregarding instructions marked with this symbol may lead to injury or property damage. 2

5 CAUTION- CLASS 1M INVISIBLE LED RADIATION WHEN OPEN DO NOT VIEW DIRECTLY WITH OPTICAL INSTRUMENTS CLASS 1 LED PRODUCT IEC/EN :2001 Meaning of the Symbols Used on the Product LED safety The light in the near-infrared region (IR wavelength) emitted from the infrared LED is applied to the focusing control. This product conforms to the EU standard EN : 2001 and the international standard IEC : Additionally, the LED safety is classified to Class 1. The safety label is attached on the upper surface of the LV-DAF intermediated tube and the safety standard/rating label is on the side of the LV-DAF controller. For fear of exposure to radiation in the light from the LED, never attempt to operate or adjust the product according to the instructions that are not indicated in this manual. Also, do not remove the cover from the product. Normally, IR light is emitted from the attachment location of the lower surface of the intermediate tube. Do not use the product as a single unit. Be sure to use it with assembled to the microscope system. Normally, IR light is not emitted from the eyepiece tube mounting location on the upper surface of the intermediate tube. Safety label Safety standard/rating label Upper surface of the intermediate tube Side of the controller Side of the intermediate tube Infrared ray emission port Symbol Detail Caution for heat The symbols on the back of the lamp house and near the lamp house clamp screw of the LV-UEPI, LV-UEPI2, and LV-UEPI2A call your attention on the following: For positions of this symbol, refer to the instruction manual of each illuminator. The lamp house becomes extremely hot while the lamp is on and immediately after it is turned off. Do not touch the lamp house during the lamp is lit and immediately after it is turned off to prevent the risk of burns. Make sure that the lamp house is sufficiently cool before the lamp replacement. Do not touch when carrying the product. The symbols on the both sides of the LV-FMA motorized focusing module elevating section call your attention on the following. For positions of this symbol, refer to the instruction manual of the LV-FMA. Do not touch the elevating section and arm when carrying the product. 3

6 WARNING 1. Intended product use The product is an equipment to perform the auto focus function (automatic focusing) with assembled to the Nikon LV series microscope. Do not use the product for other purposes. 2. Do not disassemble. Disassemble of the product may result in electric shock or malfunctions. Never attempt to disassemble any part other than the parts described in this manual. If you notice abnormalities in the product, contact your nearest Nikon representative. 3. Read the instructions carefully. To ensure safety, carefully read this manual and the manuals for other equipment used with this microscope. In particular, observe all warnings and cautions given in the first of each manual. 4. Rating of the power supply The power supply circuit in the product is designed for AC power of 100 to 240 VAC and 50/60 Hz. Before connecting the power cord, check that the power supply to be used conforms to the voltage and frequency described above. Use of a non-conforming power line may result in equipment malfunction, failure, or fire. 5. Power cord Be sure to use the specified power cord. Use of a wrong power cord may result in malfunctions or fire. Additionally, be sure to connect the product to an appropriate ground terminal (protective earth terminal) because it is classified as subject to Class I protection against electric shock. To prevent electrical shock, always turn off the power switch (press the side) before attaching or detaching the power cord. Refer to IX. Specifications for the specified power cord. 6. Object devices of the product The product can perform the auto focus function if assembled with the following devices. The product cannot perform the auto focus function if assembled with the others. Motorized focusing module: LV-FMA FM module A, LV-IMA IM module A Motorized nosepiece: LV-NU5A U5A nosepiece, LV-NU5AC U5AC nosepiece PC: Windows PC with a USB interface or Windows PC with an RS232C interface 7. Motorized devices operations The motorized devices (motorized focusing module and motorized nosepiece) can be operated by controlling the product. To prevent injury, do not touch the motorized devices during operation. Before operating the product, make sure that the motorized devices can be operated safety. Particularly, if you touch the elevating section of the motorized focusing module or the devices attached to the elevating section during performing the auto focus function, it may cause injury to hands or fingers. Do not touch the devices when you operate the LV-DAF. 8. Heat from the light source The lamp and the lamp house become extremely hot. To avoid burns, do not touch the lamp house while the lamp is lit or for thirty minutes after it is turned off. Additionally, to avoid the risk of fire, do not place fabric, paper, or highly flammable volatile materials (such as gasoline, petroleum benzine, paint thinner, or alcohol) near the lamp house while the lamp is lit or for about thirty minutes after it is turned off. 9. Cooling fan and air vents Do not block the opening for the cooling fan on the back and air vents on the side. If the cooling fan or air vents are blocked, the inside temperature of the product rises and it results in failure, malfunction, or fire. 4

7 CAUTION 1. Handle carefully. Handle the product carefully to avoid shock or impact. Do not forcefully pull or twist the power cord and the cables. Do not drop metal pieces such as clips and staples into any clearance or the product inside. It may result in failures or fire. 2. Do not wet the product. If the product gets wet, it may cause a short circuit and result in failure or abnormal heating. If the product accidentally gets wet, immediately turn off the power switch (press the side) and unplug the power cord from the wall outlet. Then, wipe off water with a piece of dry cloth. If water enters the product, immediately suspend use of the product and contact your nearest Nikon representative. 3. Weak electromagnetic wave The product emits weak electromagnetic waves. Do not place any precision electronic devices near the product to prevent its accuracy from being adversely affected. If a TV or radio reception is affected, move the TV or radio set farther from the product. 4. Installation location The product is a precision optical instrument. Therefore, use or storage of the product in an inappropriate environment may result in failures or poor performance. Consider the following requirements when arranging an installation location. Avoid bright locations where the product is exposed to direct sunlight or immediately under room lights. If the ambient light is bright, the image will deteriorate. Install the product with a surrounding clearance of 10 cm or more. Install the product in a location that is free from considerable dust or dirt. Install the product on a flat surface with little vibration. Install the product on a sturdy desk or table to avoid breakage of the product. Do not install the product in a hot and humid location. Install the product in a location where the power cord can be easily unplugged from the AC inlet in the event of an emergency. Refer to IX. Specifications for operation and storage conditions. 5. Cautions on assembling the product Be sure to turn off the power switch before assembling the product or attaching/detaching the cable. Be careful not to pinch your fingers or hands during assembly. Scratches or fingerprints on the lens will adversely affect the image. Be careful not to scratch the lens during assembly. 6. Cable routing Route the cables away from the light source and lamp house. If a cable comes into contact with the lamp house, the cable sheath may melt and it results in an electrical shock or fire. 7. Setup works after assembly Perform setup works for the product when assembling the microscope system or changing the configuration. In the setup work, register information of the objective attached to the motorized nosepiece and specify the focus position of each objective. Be sure to perform the setup work before operation to perform the auto focus function correctly. For details about the setup work, refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM. 5

8 CONTENTS WARNING and CAUTION Symbols Used in This Manual... 2 Meaning of the Symbols Used on the Product... 3 WARNING... 4 CAUTION... 5 Overview of the Product... 7 Names and Functions of Componens Intermediate Tube Controller... 9 Assembly Examples of the System Configuration Attaching the Intermediate Tube Attaching the IR Cut Lens or IR Cut Filter Connecting the Controller Connecting PCs System Configuration with the LV-ECON Connecting the Power Cord Tilt Adjustment Legs Setup Works Operation Power On/Off Selecting the Microscopy Method Auto Focus On/Off Selecting an Objective AF Offset Function Search Function Manual Focusing External Control RS232C communication Control USB communication Control Digital Input/Output Control Troubleshooting Care and Maintenance Cleaning Lenses and Filters Cleaning the Painted Parts, Plastic Parts, and Printed Parts Storage Regular Inspections Specifications

9 CAUTION w e n n A b d e c k u n g g e o f f n e t. N i c h t d e m S t r a h l a u s s e t z e n. L E D K l a s s e 3 b Overview of the Product Overview of the LV-DAF The Nikon LV-DAF Dynamic Auto Focus is the product to perform the auto focus function (automatic focusing) with assembled to the Nikon LV series motorized microscope system. The product is controlled to focus on specimens by projecting the slit image to be used for focusing on the specimen using IR light (near-infrared light) and detecting its reflection. The focus position is controlled by the hybrid method of the slit projection method and the slit image contrast detection method so that the focus range can be enlarged and the auto focus function can be performed for both diascopic illumination and episcopic illumination. To perform the auto focus function using this product, the following motorized devices must be assembled to the microscope system. Motorized focusing module (LV-FMA or LV-IMA) Motorized nosepiece (LV-NU5A or LV-NU5AC) This product can be assembled to the motorized microscope system accommodating the LV-ECON E controller. When using the LV-ECON, the motorized devices operations other than focus function is performed with the LV-ECON and the auto focus and manual focus functions are performed with the controller of this product. Configuration This product includes of the following devices. Controller Intermediate tube IR cut lens (for the episcopic illuminators) Connecting cable (between the controller and intermediate tube) RS232C cable (for connecting PC or LV-ECON) USB cable (for connecting PC or DS-L2) Power cord * LED RADIATION WHEN OPEN. AVOIF EXPOSURE TO BEAM. CLASS 3b LED V o r s i c h t u n s i c h t b a r e L E D S t r a h l u n g, IR cut lens (for the episcopic illuminators) Controller Intermediate tube Connecting cable (between the controller and intermediate tube) RS232C cable (for connecting PC or LV-ECON) USB cable (for connecting PC or DS-L2) Power cord * * The power cord specification depends on the region to be used. 7

10 CAUTION V o r s i c h t Names and Functions of Components 1 Intermediate Tube 5 1 LED RADIATION WHEN OPEN. AVOIF EXPOSURE TO BEAM. CLASS 3b LED u n s i c h t b a r e L E D S t r a h l u n g, w e n n A b d e c k u n g g e o f f n e t. N i c h t d e m S t r a h l a u s s e t z e n. L E D K l a s s e 3 b Eyepiece tube mounting part An eyepiece tube is mounted here. A fixing clamp screw is attached in the front of this device. 2 Mounting part (circular dovetail joint) This is the circular dovetail joint to attach this device onto another device. 3 Slot for analyzer slider An analyzer slider is inserted when performing the polarization microscopy or DIC microscopy. When not using the analyzer slider, insert the provided dummy slider into this slot. 4 Connector This connector is used to connect the controller with the provided cable. 5 Warning label CAUTION- CLASS 1M INVISIBLE LED RADIATION WHEN OPEN DO NOT VIEW DIRECTLY WITH OPTICAL INSTRUMENTS CLASS 1 LED PRODUCT IEC/EN :2001 8

11 II. Names and Functions of Components 2 Controller Controller front view Power indicator (green) The indicator is lit when the product is energized. 7 AUTO FOCUS control panel It is used to turn on and off the auto focus function. The indicator shows the focusing conditions. 8 OBJ. control panel It is used to rotate the motorized nosepiece to change objectives. 9 AF OFFSET control panel It is used to move the elevating section of the motorized focusing module and change the focus position under the auto focus condition. 10 FOCUS control panel It is used to move the elevating section of the motorized focusing module when the auto focus function is turned off or when the auto focus function is turned on but the DISABLE indicator is lit. 11 SEARCH control panel It is used to search focus positions. 12 MIC button It is used to set the microscopy method to be performed. 13 AUX. control panel Not used. 14 Tilt adjustment legs They are used to adjust the tilt angle of the product. 9

12 II. Names and Functions of Components Controller rear view USB SENSOR SERIAL I/O LV-FMA V~ 1.2A 50/60Hz NOSEPIECE Switch cover (on the side) This cover is removed when adjustment switches inside are used. Normally, do not remove the cover. 16 USB connector It connects the devices such as the PC for setup work or the PC for external control. (This interface complies with USB 1.1.) 17 SENSOR connector It connects with the LV-DAF intermediate tube using the provided connecting cable. 18 SERIAL connector It connects the devices such as the PC for setup work or the PC for external control. When used with the motorized microscope system accommodating the LV-ECON, use this connector to connect this device and the LV-ECON. 19 I/O connector It is used for digital input and out control. 20 LV-FMA connector It is used to connect the motorized focusing module (Nikon LV-FMA or LV-IMA). 21 NOSEPIECE connector It is used to connect the motorized nosepiece (Nikon LV-NU5A or LV-NU5AC). When used with the motorized microscope system accommodating the LV-ECON, do not connect the motorized nosepiece to this connector. Connect the motorized nosepiece to the LV-ECON. 22 Power switch This is the power switch of this product. 23 AC inlet It is used to connect to the AC power supply. Be sure to use the specified power cord. 24 Cooling fan This is the cooling fan of this product. 10

13 Assembly The procedures for assembling and connecting the product are described in this chapter. Refer to the instructions in this chapter for connection of the devices. Refer to the instruction manual of each device for installation and assembly. WARNING Before connecting devices, be sure to read the WARNING and CAUTION in the first of this manual and follow the instructions written therein. To avoid electrical shock or fire, be sure to turn off the power of the product (press the side of the power switch) before unplugging the power cord. CAUTION Be careful not to pinch your fingers or hands during assembly. Scratches or fingerprints on the lens will adversely affect the image. Be careful not to scratch the lens during assembly. If lenses are contaminated with fingerprint or such, clean them according to the procedure described in VIII. Care and Maintenance. This product is a precision optical instrument. Handle the product carefully and do not subject it to a strong physical shock. Especially, the accuracy of the objective may be adversely affected by even a slight damage. Route the cables away from the illuminator or lamp house. If a cable comes into contact with the lamp house, the cable sheath may melt and it results in an electrical shock or fire. CAUTION Setup work for the Auto Focus Perform the setup work for the product after assembling the microscope or changing the configuration. In the setup work, register information of the objective attached to the motorized nosepiece and specify the focus position of each objective. Make sure to perform the setup work before operation to perform the auto focus function correctly. Refer to ECLIPSE LV Series Support Tools Software manual in the provided CD- ROM. 11

14 Do not touch the height adjustment part when carrying this product. MIN. MAX. POWER 1 Examples of the System Configuration This section shows the configuration example of the microscope system with the LV-DAF. System configuration example for episcopic illumination Eyepiece Eyepiece tube Clamp for the eyepiece tube Clamp for the intermediate tube LV-DAF intermediate tube * Clamp for the illuminator (x4) Clamp for the lamp house Lamp house LV-LH50PC Illuminator LV-UEPI IR cut lens * Clamp for the illuminator Motorized focusing module LV-FMA or LV-IMA Lamp cable Motorized nosepiece LV-NU5A Objective Special connecting cable Special connecting cable Power supply (This device needs a power supply for its own.) Special connecting cable * LV-DAF Controller * (This device needs a power supply for its own.) RS232C cable * or USB cable * USB cable * PC (This device needs a power supply for its own.) The device indicated with the * symbol is included in the product. DS Camera Control Unit DS-L2 (This device needs a power supply for its own.) 12

15 Do not touch the height adjustment part when carrying this product. MIN. MAX. POWER MIN. MAX. POWER III. Assembly System configuration example for diascopic illumination Eyepiece Eyepiece tube Clamp for the eyepiece tube Clamp for the intermediate tube Clamp for the illuminator Motorized nosepiece LV-NU5A LV-DAF intermediate tube * Clamp for the illuminator (x4) Illuminator LV-UEPI IR cut lens * Motorized focusing module LV-FMA Clamp for the lamp house Lamp house LV-LH50PC Lamp cable Power supply TE2-PS100W (This device needs a power supply for its own.) IR cut filter Objective Clamp for the lamp house Lamp house LV-LH50PC Microscope base for the diascopic illumination LV-DIA Lamp cable Power supply TE2-PS100W (This device needs a power supply for its own.) Special connecting cable Special connecting cable Special connecting cable * LV-DAF Controller * (This device needs a power supply for its own.) RS232C cable * or USB cable * PC (This device needs a power supply for its own.) USB cable * The device indicated with the * symbol is included in the product. DS Camera Control Unit DS-L2 (This device needs a power supply for its own.) 13

16 CAUTION w e n n A b d e c k u n g g e o f f n e t. N i c h t d e m S t r a h l a u s s e t z e n. L E D K l a s s e 3 b 2 Attaching the Intermediate Tube Attach the intermediate tube of the LV-DAF to the microscope system. The procedure is as follows: 1 Check that the motorized focusing module (LV-FMA or LV-IMA) is secured to the installation position. 2 Attach the motorized nosepiece (LV-NU5A or LV-NU5AC) to the motorized focusing module. Refer to the instruction manual of the LV-FMA or LV-IMA for details about the motorized nosepiece attachment method. 3 To use the episcopic illuminator, mount it on the arm of the motorized focusing module. 4 When using the episcopic illuminator, mount the intermediate tube of the LV-DAF on the episcopic illuminator and secure it using a clamp screw. When not using the episcopic illuminator, mount the intermediate tube of the LV-DAF on the arm of the motorized focusing module and secure it using a clamp screw. 5 Attach the eyepiece tube to the upper side of the intermediate tube of the LV-DAF and secure it using a clamp screw. 6 Attach objectives to the motorized nosepiece so that the magnification increases as the nosepiece is rotated clockwise as viewed from above. Eyepiece tube IN OUT LED RADIATION WHEN OPEN. AVOIF EXPOSURE TO BEAM. CLASS 3b LED V o r s i c h t u n s i c h t b a r e L E D S t r a h l u n g, LV-DAF intermediate tube Clamp screw BF DF F.STOP A.STOP Clamp screw Episcopic illuminator Do not touch the height adjustment part when carrying this product. Motorized nosepiece Motorized focusing module LV-FMA or LV-IMA Objective Refer to the instruction manual of each device for assembly. 14

17 Do not touch the height adjustment part when carrying this product. III. Assembly 3 Attaching the IR Cut Lens or IR Cut Filter The product controls the focusing conditions using the near-infrared (IR) light. Therefore, to operate the product correctly, IR light needs to be removed from the illumination. Attach the IR cut lens or IR cut filter as follows: For episcopic illumination To perform episcopic illumination with the episcopic illuminator, attach the provided IR cut lens to the lamp house connector part of the illuminator. WARNING In the following procedure, the lamp house is attached or detached. To prevent the risk of burns, make sure that the lamp is turned off and its surrounding area are cooled down sufficiently before operation. 1 When the lamp house is attached to the microscope system, make sure that the lamp is turned off and the lamp house is cooled down sufficiently. Then, remove the lamp cable from the lamp house and loosen the clamp screw to remove the lamp house. 2 Insert the IR cut lens into the lamp house connector part of the episcopic illuminator (LV-UEPI, LV-UEPI2, or LV-UEPI2A). Insert the IR cut lens as far as it goes with the lens facing inside of the microscope. 3 Attach the lamp house and secure it using a clamp screw. IR cut lens 4 Connect the lamp cable to the power supply. IR cut lens Lamp house Refer to the instruction manual of the episcopic illuminator (LV-UEPI, LV-UEPI2, or LV- UEPI2A) for details about installation method of the lamp house and replacement method of lamps. For diascopic illumination To perform diascopic illumination with the LV-DIA DIA base, attach the optional IR cut filter on the field lens of the LV-DIA. IR cut filter F.S. 15

18 Do not touch the height adjustment part when carrying this product. 4 Connecting the Controller Connect the intermediate tube of the LV-DAF, the motorized focusing module, and the motorized nosepiece to the controller of the LV-DAF using the special connecting cables. 1 Make sure that the power switch on the back of the LV-DAF controller is turned off (the side is pressed down). 2 Connect the connector on the side of the intermediate tube and the SENSOR connector on the back of the controller using the special connecting cable. 3 Connect the connector on the side of the motorized focusing module and the LV-FMA connector on the back of the controller using the special connecting cable. 4 Connect the cable of the motorized nosepiece with the NOSEPIECE connector on the back of the controller using the special nosepiece connecting cable. LV-DAF intermediate tube Motorized nosepiece LV-NU5A or LV-NU5AC Motorized focusing module LV-FMA or LV-IMA Connecting cable Connecting cable SENSOR SERIAL I/O LV-FMA USB V~ 1.2A 50/60Hz NOSEPIECE LV-DAF controller Nosepiece Connecting cable * The length of each connecting cable is 2.8 m. An optional connection cable with a length of 10 m is also available. 16

19 USB USB SENSOR SERIAL I/O LV-FMA NOSEPIECE SENSOR SERIAL I/O LV-FMA NOSEPIECE III. Assembly 5 Connecting PCs Connecting a PC for the setup work To ensure correct operation, specify the information of objectives via a PC according to the special setup software LV-DAF Setup. Use the RS232C interface or USB interface to connect the product to the PC. After assembling and connecting the product, connect the PC with the controller using the provided RS232C cable (crosswired type cable) or USB cable. And then, activate the LV-DAF Setup to specify the information of objectives. Refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM for details about specifying the information of objectives. For the RS232C interface application LV-DAF controller V~ 1.2A 50/60Hz PC RS232C cable (cross-wired type cable) For the USB interface application V~ 1.2A 50/60Hz PC LV-DAF controller USB cable * The USB interface of the product is compatible with USB

20 USB USB SENSOR SERIAL I/O LV-FMA NOSEPIECE SENSOR SERIAL I/O LV-FMA NOSEPIECE Connecting a PC or other device for external control The product can be controlled with a PC or other device using the USB interface or RS232C interface. Refer to the following figures: For the USB interface application V~ 1.2A 50/60Hz PC or other device LV-DAF controller USB cable * The USB interface of the product is compatible with USB 1.1. For the RS232C interface application LV-DAF controller V~ 1.2A 50/60Hz PC or other device RS232C cable (cross-wired type cable) 18

21 Do not touch the height adjustment part when carrying this product. MIN. MAX. MIN. MAX. POWER POWER INTENSILIGHT C-HGFIE RUN TIME hrs. LAMP POWER III. Assembly 6 System Configuration with the LV-ECON The LV-ECON E controller is the controller for operating all motorized devices (LV series). When applying the product to the motorized microscope system accommodating the LV-ECON, change the connection as follows: Connect the motorized nosepiece to the LV-ECON. Connect the LV-ECON and the LV-DAF controller using the RS232C interface. Connect the LV-ECON to the PC or DS-L2 using the USB interface. Refer to the following diagram for connection. Eyepiece Eyepiece tube LV-TT2 LV-DAF Intermediate tube Optical fiber adapter LV-HGFA Light guide fiber External Light source * C-HGFIE Or Episcopic illumination LV-UEPI2A Lamp house for episcopic illumination LV-LH50PC Lamp cable Motorized nosepiece LV-NU5A Focusing module LV-FMA Lamp cable Power supply * TE2-PS100W When the LV-DIA is applied. Lamp house for diascopic illumination LV-LH50PC Special connection cable Special connection cable Microscope base LVDIA or LV-EPI Lamp cable Lamp cable Power supply * TE2-PS100W LV-DAF controller * RS232C cable LV-ECON * USB cable PC * USB cable DS Camera Control Unit DS-L2 * * These devices must be connected to their own power supplies. 19

22 III. Assembly 7 Connecting the Power Cord WARNING Be sure to use the specified power cord. Use of a wrong power cord may result in malfunction or fire. Additionally, be sure to connect the product to earth ground (protective earth terminal) because it is classified as Class I protection against electric shock. Refer to IX. Specifications for details of the power cord. Make sure that the power switch is turned off (the side is pressed down). Insert the socket into the AC inlet on the back of the device. Then, securely plug the power cord to a wall outlet. 8 Tilt Adjustment Legs The tilt adjustment legs for changing the tilt angle of the product are attached to the front legs part of the product. Check the installation condition of the product, and then use the tilt adjustment legs if you wish. 20

23 Setup Works The LV-DAF Dynamic auto focus system must be set up after the following cases: The system is used for the first time. The objective configuration is changed in the microscope. The specimen is changed. The controller is changed. Or, the intermediate tube is changed. To perform the setup works, use the provided software LV-DAF Setup on a PC. Refer to the ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM for details about LV-DAF Setup operation and setup works. CAUTION If the setup work is not performed, this product does not work correctly. Be sure to set up the product. 21

24 Operation WARNING Each motorized device will move when the product is operated. To prevent injury, do not touch the motorized devices during operation. Before operating the product, make sure that the motorized devices can be operated safety. 1 Power On/Off Power On/Off The power switch is located beside the AC inlet on the rear. To turn on the product, press the I side of the power switch. To turn off the product, press the side of the power switch. When the product is turned on, the POWER indicator on the front of the product lights green. Initialization of the motorized devices When the product is turned on, a beep sounds, all indicators on the operation panel turn off, and the product starts communications with each motorized device. When the communication is established, each motorized device is set to the predetermined conditions and the indicators on the operation panel show the current condition. This procedure is called the initialization of the microscope system. When the initialization ends, a beep sounds again to inform that the product is ready for use. POWER indicator SENSOR SERIAL I/O LV-FMA USB V~ 1.2A 50/60Hz NOSEPIECE Front view LV-DAF controller Power switch Rear view 22

25 V. Operation 2 Selecting the Microscopy Method To perform the auto focus function, the setting of the product must be changed suitable for the microscopy method. Press the MIC button on the front of the controller and select the microscopy method performed on the microscope. Selection of the microscopy method Indicator BF DF OP Microscopy method Bright-field microscopy under the episcopic illumination Dark-field microscopy under the episcopic illumination/bright-field microscopy under the diascopic illumination Option For the option setting, register the focusing conditions of objectives of your desired microscopy method such as polarized light microscopy and DIC microscopy. Refer to ECLIPSE LV Series Support Tools Software Manual in the provided CD-ROM for details. 3 Auto Focus On/Off To start the auto focus function, press the ON button of the AUTO FOCUS control panel and light up the indicator. During focusing, the indicators on upper and lower of the JUST FOCUS indicator show focusing conditions, and the JUST FOCUS indicator is lit when the system focuses on the specimen. The DISABLE indicator is lit when this system cannot focus on the specimen using the auto focus function. To turn off the auto focus function, press the ON button and turn off the indicator. Auto Focus On/Off 23

26 CAUTION LED RADIATION WHEN OPEN. AVOIF EXPOSURE TO BEAM. CLASS 3b LED V o r s i c h t u n s i c h t b a r e L E D S t r a h l u n g, w e n n A b d e c k u n g g e o f f n e t. N i c h t d e m S t r a h l a u s s e t z e n. L E D K l a s s e 3 b 4 Selecting an Objective To select an objective, press the + / button of the OBJ. control panel to rotate the motorized nosepiece. The 1 to 5 indicators of the OBJ. control panel show the nosepiece address of the objective in the optical path. Selecting an objective The motorized nosepiece rotates as described below by operating the + / button of the OBJ. control panel. Operation of the motorized nosepiece Operation Motorized nosepiece Objective magnification* + button Rotate the nosepiece clockwise as viewed from above. (forward rotation) The magnification increases. button Rotate the nosepiece counterclockwise as viewed from above. (reverse rotation) The magnification decreases. IN OUT During the rotation of the motorized nosepiece, all indicators are turned off temporarily. * Attach the objectives to the nosepiece in ascending order. (The nosepiece address 1 is the lowest magnification and the nosepiece address 5 is the highest magnification.) Do not touch the height adjustment part when carrying this product. Limitation in selecting an objective The following limitation can be applied to the operation of the motorized nosepiece with the LV- DAF Setup software. You can disable the rotation of the nosepiece not to change objectives from a low magnification objective 5x or lower to a high magnification objective of short working distance less than 1 mm. 24

27 V. Operation 5 AF Offset Function After focusing on the specimen with the auto focus function, focal position can be shifted with remained in the auto focus function. This operation is called AF offset. The offset value of each magnification (each address of the motorized nosepiece) can be registered. To perform the AF offset function, press the ON button of the AF OFFSET control panel and light up the indicator after focusing on the specimen with the auto focus function. Then, operate + / button of the AF OFFSET control panel to move the elevating section of the motorized focusing module so that the focal position can be moved. Operation of the AF OFFSET On/Off Operation + button button Elevating Section Up Down Objective The objective moves farther from the specimen. The objective moves closer to the specimen. To turn off the auto focus function, press the ON button again and turn off the indicator. When the AF offset function is turned off, the offset values are reset. Note the followings: When the indicator is lit and the specimen is in focus, the AF offset function is running. When the AF offset function is running, the performance of auto focus will be less good than the normal condition. 25

28 6 Search Function If the specimen does not come into focus with the auto focus function after manual focusing or such operation, execute the search function to search a focusing position automatically. The search function has Single mode and Continuous mode. Execution of the search function Single mode On this mode, the search function ends when a focusing position is found. To invoke the single mode, press the ON button in the SEARCH control panel while the DISABLE indicator in the AUTO FOCUS control panel is lit. The indicator in the SEARCH control panel turns on. When the search function starts, the motorized focusing module moves upward and searches a focusing position. When a focusing position is found, the search function ends. If no focusing position is found, the DISABLE indicator in the AUTO FOCUS control panel turns on. Continuous mode On this mode, the auto focus function starts automatically when a focusing position is found. To invoke the continuous mode, press the ON button in the AUTO FOCUS control panel while the DISABLE indicator in the AUTO FOCUS control panel is lit. The AF indicator in the AUTO FOCUS control panel turns on. And then, press the ON button in the SEARCH control panel. The indicator in the SEARCH control panel turns on. When the search function starts, the motorized focusing module moves upward and searches a focusing position. When a focusing position is found, the auto focus function automatically starts. If no focusing position is found, the DISABLE indicator in the AUTO FOCUS control panel turns on. In the initial setting, the motorized focusing module moves only in the upward direction. But if the focusing position cannot be searched even when the module moves to the upper limit position, the module can be set to move in the downward direction. Additionally, when the module is set to move in the downward direction, be careful of a collision of the objective with the specimen. To prevent the collision, set up the software limit with LV-DAF Setup in the provided CD-ROM. 26

29 CAUTION w e n n A b d e ckung g e o f f n e t. N i c h t d em S t r a h l a u s s e t z e n. L E D K l a s s e 3 b V. Operation 7 Manual Focusing When the Auto Focus function is off, that is to say, when the AF indicator is turned off or both of the AF indicator and the DISABLE indicator are turned on, the manual focusing can be performed by operating the buttons of the FOCUS control panel. By operating the + / button of the FOCUS control panel, the indicator is lit and then the elevating section of the motorized focusing module can be moved manually. To change the focusing mode between the fine focusing and the coarse focusing, press the FINE buttom. In the fine focusing mode, the FINE indicator turns on. In the coarse focusing mode, the indicator turns off. The elevating section of the motorized focusing module moves as described below by operating + / button. Operation of the manual focusing IN OUT Coarse focusing / fine focusing LED RADIATION WHEN OPEN. AVOIF EXPOSURE TO BEAM. CLASS 3b LED V o r s i c h t u n s i c h t b a r e L E D S t r a hlung, Do not touch the height adjustment part when carrying this product. Operation + button button Elevating Section Up Down Objective The objective moves farther from the specimen. The objective moves closer to the specimen. With the elevating section is located at the upper or lower limit position and it is attempted to move farther, a beep sounds. * The vertical movable range of the elevating section of the LV-FMA and LV-IMA is approximately 20 mm. When using the LV-ECON, the limit positions for vertical movement can be set with LV-DAF Setup. 27

30 External Control 1 RS232C Communication Control With the RS232C communications, the LV-DAF components can be set up, driven, and read for their conditions. For details about the communication specifications, refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM. Communication type Start-stop serial communication (half-duplex communication) Connector specification Marking: SERIAL Type: D-SUB 9-pin male RS232C connector Pin number Signal Input/output RxD TxD DTR SG DSR Input Output - GND : not used Caution: Flow control with control signals, DTR and DSR, cannot be used in the communications of the product. Signal connections Example for 9-pin connector device signal connections LV-DAF (SERIAL) User's device 1 1 RxD 2 2 RxD TxD 3 3 TxD 4 4 GND 5 5 GND

31 VI. External Control 2 USB Communication Control Communication type: USB 1.1 For details about the communication specifications, refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM. 3 Digital Input/Output Control With the digital parallel input/output control from an external device, some LV-DAF components can be set up and read for their conditions. Photocoupler circuits are recommended to improve noise immunity of the signals. Connector specification Marking: I/O Connector type on the product: HR212-10RC-10SDL manufactured by HIROSE ELECTRIC CO., LTD. Connector type on the cable: HR 12 series, 10-element connector manufactured by HIROSE ELECTRIC CO., LTD. Signal specification Pin number Signal Input/output Remark 1 AN Input Anode signal for LEDs in the photocoupler 2 DGND Digital ground 3 EMSTP Input Emergency stop signal for vertical movement 4 AF ON/OFF Input ON/OFF signal for auto focus function 5 +5V Output +5V Vdd output 6 DGND Digital ground 7 JF Output Focus condition signal 8 AFENBL Output Auto focus condition signal : not used 29

32 Signal details Pin number Signal AN DGND EMSTP AF ON/OFF +5V DGND JF AFENBL Description Anode signal (positive voltage) for LEDs in the photocoupler in the AF controller Note that no current limit resistor is built in. Digital ground Input signal to stop the vertical movement in emergency Signal status; Low: emergency stop (The vertical movement stops with an electric method.) High: normal status To restore, set High for this signal, and then turn off and on the power of the product or input the cancel command. When the cancel command is sent, the auto focus function and the vertical movement are initialized. Input signal to turn on/off the auto focus function Signal status:the condition toggles when the signal changes from High to Low. Example: From AF ON (auto focus condition) to AF OFF (manual focus condition) and the other way around. +5 V output Vdd output of external photocouplers for 7- and 8-pin Digital ground Output signal of focus condition Signal status; Low: focusing (The JUST FOCUS indicator on the operation panel turns on.) High: not focusing (The JUST FOCUS indicator on the operation panel turns off.) Output signal of auto focus condition Signal status; Low: auto focus function ON (The AF indicator on the operation panel turns on.) High: auto focus function OFF (The AF indicator on the operation panel turns off.) Not used. Not used. 30

33 VI. External Control Electrical specification CONNECTOR [I/O] Photocoupler Fixed resister Photocoupler DGND +5V DGND Driver IC Driver IC 470Ω 470Ω Pin number Signal AN DGND EMSTP AF ON/OFF +5V DGND JF AFENBL Description Photocoupler LED forward current: 80 ma or less (14 to 20 ma recommended) 0 V Reverse voltage: 4 V or less Photocoupler LED forward current: 40 ma or less (7 to 10 ma recommended) Reverse voltage: 4 V or less Photocoupler LED forward current: 40 ma or less (7 to 10 ma recommended) Output voltage: +5 V ±3% Output current: 100 ma or less 0 V Driver IC output voltage VOH: 4.4 V or more (at output current 50 μa) Driver IC output voltage VOL : 0.5 V or less (at output current 8 ma) Output voltage: -0.3 to +5.3 V Output current IOL: 15 ma or less (7 to 10 ma recommended) Driver IC output voltage VOH: 4.4 V or more (at output current 50 μa) Driver IC output voltage VOL: 0.5 V or less (at output current 8 ma) Output voltage: -0.3 to +5.3 V Output current IOL: 15 ma or less (7 to 10 ma recommended) Not used. Not used. 31

34 Troubleshooting Improper use of the product may adversely affect performance, even if the microscope system is not damaged. If any of the troubles listed in the table below arise, take the countermeasures indicated. Trouble Unable to turn on the product. Unable to focus on the specimen by the auto focus function. The viewfield is invisible, vignetted, or uneven in brightness. Soil or dust is seen in the viewfield. A motorized device does not work. Sometimes the nosepiece does not rotate. Unable to operate using the controller correctly. Cause The power cord is not connected. Specimens other than the one to be detected are observed. Specimen is out of the focusing available range. Setup works have not been performed. Or the setting is wrong. Near-infrared radiation is included in the optical path of illumination light. A device, lens, lamp, or filter is not attached correctly. A filter or a slider is located in an intermediate position. Soil or dust exists on the lens, filter, or specimen. The motorized device is not connected correctly. A limitation is applied to the rotation of the nosepiece. The setup work is not completed. The device is not connected correctly. Connect it properly. Countermeasure (p.20) Observe the transmission or nontransmission specimens with the boundary surface whose reflectance is 4% or more. Place the specimen in the focusing available range. Perform the setup works and specify the settings properly. (Refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM.) Attach the IR cut lens. (p.15) Attach the devices to the correct positions. (p.12 to 15) Move the filter slider to a click-stop position. Clean the lens, filter, or specimen. (p.34) Connect the motorized device properly. (p.11 to 20) Clear the limitation. (Refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM.) Perform the setup works and specify the settings properly. (Refer to ECLIPSE LV Series Support Tools Software manual in the provided CD-ROM.) Connect the device properly. (p.11 to 20) 32

35 Care and Maintenance Nikon recommends daily care and maintenance for maintaining the performance as long as possible. Do not let dust, fingerprint, etc. get on the lenses. Soil on the lens or filter adversely affects the optical performance of the microscope. If lenses are contaminated, clean them according to the procedure described in 1. Cleaning Lenses and Filters. When cleaning, be sure to turn off the power switch of the microscope (press the side) to avoid malfunction. Daily care and maintenance Clean the parts frequently manipulated by hands, such as eyepieces and glass plate, according to the procedure described in 1. Cleaning Lenses and Filters without removing them from the microscope. Nikon recommends cleaning them frequently. Clean the tip of the objectives and filters to maintain the optical performance. When cleaning the objectives, remove them from the microscope. Clean them whenever they are contaminated. Microscopes and stages are contaminated with use. When you find the microscope is contaminated, clean them according to the description in 2. Cleaning the Painted Parts, Plastic Parts, and Printed Parts. Cleaning tool and supplies (consumables) Cleaning tool Brush (with soft bristles) (Use a cleanroom wiper in a clean room.) Cleaning supplies (consumables) Ethyl or methyl alcohol Lens tissue (Use a cleanroom wiper in a clean room.) 33

36 VIII. Care and Maintenance 1 Cleaning Lenses and Filters Do not let dust, fingerprint, and so on get on the lenses and filters. Soil on the lens or filter adversely affects the viewing of the image. If any of the lenses get dirty, clean them as described below. Either brush away dust with a soft brush, or else gently wipe it off with a piece of gauze. Only if there are fingerprints or grease, dampen a piece of soft, clean cotton cloth, lens tissue, or gauze lightly with absolute alcohol (ethyl or methyl) and gently wipe off the soil. Absolute alcohol is highly flammable. Be careful when handling it, when around open flames, when turning the power switch on/off, and so on. Follow the instructions provided by the manufacturer when using absolute alcohol. 2 Cleaning the Painted Parts, Plastic Parts, and Printed Parts Do not use organic solvents such as alcohol, ether, or paint thinner on painted components and plastic components. Doing so could result in discoloration or in peeling of the printed characters. For persistent soil, dampen a piece of gauze with neutral detergent and wipe lightly. 3 Storage Store the product in a dry place where mold is not likely to form. Store the objectives and eyepieces in a desiccator or similar container with a drying agent. Put a vinyl cover over the product to protect it from dust. Before putting on the vinyl cover, turn off the power switch of the microscope system (press the side) and wait until the lamp house gets cool sufficiently. 4 Regular Inspections Periodical inspections of the product are recommended in order to maintain peak performance. Contact your nearest Nikon representative for details. 34

37 Specifications LV-DAF Model Type Applicable specimens Applicable objective Controllable devices Interface connectors Input rating Power cord Operation conditions Storage conditions LV-DAF Dynamic Auto Focus Detection method: AF light source: Light receiving element: AF mode: Transmission specimen: Nontransmission specimen: 2.5x, 5x, 10x, 20x, 50x, and 100x Functions are limited for some objectives. Motorized focusing module: Motorized nosepiece: PC: Slit projection contrast detection method (Hybrid method composed of the slit projection method and the slit image contrast detection method) Near-infrared LED (λ = 770 nm) One dimension CCD Continuous mode/single mode Boundary surface with a reflectance of 4% or more (interval: 0.4 mm or more) Plane specimen with a reflectance of 4% or more LV-FMA FM module A LV-IMA IM module A LV-NU5A U5A nosepiece LV-NU5AC U5AC nosepiece Windows PC with an USB interface or Windows PC with an RS232C interface Intermediate tube to controller connector: special interface Motorized focusing connector: special interface Motorized nosepiece connector: special interface Connector for external devices: special interface PC/LV-ECON connector: RS232C interface Connector for a PC: USB 1.1 interface 100 to 240 VAC, 1.0 A, 50/60 Hz When using the product in Japan: PSE approved detachable power cord set (3 conductor grounding Type HVCTF, 1.25 mm 2, 3 m long maximum, rated at 125 VAC minimum) When the supply voltage is 100 to 120 V (Japan excluded): UL listed detachable cord set (3 conductor grounding Type SVT, No.18 AWG, 3 m long maximum, rated at 125 VAC minimum) When the supply voltage is 220 to 240 V: Approved according to EU/EN standards (3 conductor grounding Type H05VV-F, 3 m long maximum, rated at 250 VAC minimum) Temperature: 22 C ±3 C Humidity: 85 % RH maximum (no condensation) Altitude: 2000 m maximum Pollution degree: Degree 2 Installation category: Category II Electric shock protection class: Class I Indoor use only Temperature: Humidity: -20 C to +60 C 90% RH maximum (no condensation) 35