New generation of patent sequence databases Information Sources in Biotechnology Japan
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1 New generation of patent sequence databases Information Sources in Biotechnology Japan EBI is an Outstation of the European Molecular Biology Laboratory.
2 Patent-related resources Patents Patent Resources 2
3 Patent resources at EBI 3
4 Patent resources at EBI EPO Patent proteins: USPTO JPO KIPO Patent nucleotides: ENA (EPO, USPTO, JPO, KIPO) 4 Same sequences (EPO, USPTO, JPO, KIPO) Non-redundant sequence data Patent family classification Enriched with patent information
5 Sequence data from patent literature JPO USPTO NCBI GenBank NIG DDBJ KIPO INSDC 5 other patent offices INSDC agreement: Free unrestricted access All data exchanged daily EBI EMBL-Bank EPO NR patent sequence databases
6 Non-redundant patent databases Patent nucleotides Patent proteins Level-1 NRNL1 NRPL1 (Non-redundant (Non-redundant nucleotide level-1) protein level-1) Groups together 100% identical patent sequences Level-2 NRNL2 (Non-redundant NRPL2 (Non-redundant Groups together identical sequences nucleotide level-2) protein level-2) by patent family 6
7 Patent sequence record in NRNL1 7 Patents containing 100% identical sequence Sequence
8 8 Patent sequence record in NRNL2 Patent equivalents Sequence record in ENA Priority number and date Patent literature Translation Sequence
9 Non-redundant patent databases EMBL patents (redundant) Remove sequence redundancy Level-1 NR Group by patent families Additional annotation, including priority dates for patent families 9 Level-2 NR
10 Patent sequence records at EBI Nucleotide ENA NRNL1 NRNL2 ~23.9 M PAT sequences (>230 M total) ~12.2 M sequences ~15.5 M sequences Protein Patent Proteins NRPL1 ~6.5 M PRT sequences (>32 M total) ~2.5 M sequences 10 NRPL2 ~3.8 M sequences
11 11 Sequence search
12 Sequence searching Tools Sequence Similarity & Analysis 12
13 Sequence searching Wide variety of search tools 13
14 Choosing the right search engine BLAST General search engine FASTA Better general search engine SSEARCH Sensitive but slow; good for short sequences GGSEARCH Force full-length matches Query Subject 14 GLSEARCH Match domains/patterns to protein; oligo-to-gene Query Subject
15 15 Search a variety of databases Protein *Select all 6 results in triplicate!! Patent databases
16 16 Search a variety of databases Nucleotide *Select all 3 results in triplicate!! Patent data
17 17 let s look at an example
18 Searching a redundant database Protein Example: Search patent protein sequence Patent proteins 18
19 19 Results from a redundant database. >260 identical results too much to analyze
20 20 LEVEL-1 NR patent sequence database removes redundancy fewer results to analyze, less chance of missing important results
21 Searching NR level-1 patent database NR patent Level-1 Example: Search patent protein sequence NR patent level
22 22 Results from NR level-1 database Each hit unique
23 23 Results from NR level-1 database List of all patents containing the sequence Earliest publication date Link to sequence entry Link to patent documentation
24 24 Patent families Simple Patent Family is a group of patents that relate to the same invention, and are based on the same originating application They arise when an invention is patented in multiple countries Grouping patents into families reduces multi-national results down to a representative member
25 Patent families patent family Invention A second patent family Invention B EP WO US US JP GM ADA42650 CS ACQ13114 DI HB AAR79155 DD % identical sequences Same sequence can appear multiple times in a database due to: Same invention filed multiple times in different offices (same patent family) Different inventors use the same sequence in different contexts (different 25 patent families)
26 26 LEVEL-2 NR patent sequence database groups identical sequences by patent family provides earliest priority date for family
27 Searching NR level-2 patent database NR patent Level-2 Example: Search patent protein sequence NR patent level
28 28 Results from NR level-2 database Each hit = one family
29 29 Results from NR level-2 database Patent equivalents Earliest publication data in family Earliest active priority date in family
30 30 Results from NR level-2 database patents in same family Link to sequence entry Link to patent documentation
31 31 Text search
32 SRS: advanced text search 1 st : Select resources to search 2 nd : Create query 32
33 SRS: advanced text search Select library tab Sequence Searching Tools 33
34 SRS: advanced text search Search >100 databases Select library tab NR patent DNA (NRNL1 & NRNL2) NR patent proteins (NRPL1 & NRPL2) Sequence Searching Tools 34
35 SRS: advanced text search Search >100 databases Select library tab Example: Selected to search NR level-1 patent DNA database Sequence Searching Tools 35
36 SRS: advanced text search Select library tab Select resources to search Sequence Searching Tools 36
37 SRS: advanced text search Select library tab Select resources to search 1) Select field 2) Type in text Sequence Searching Tools 37
38 SRS: advanced text search Select library tab Select resources to search Sequence Searching Tools 38 Here, selected patent number
39 SRS: advanced text search Select library tab Select resources to search Create query Sequence Searching Tools 39
40 SRS: advanced text search Select library tab Select resources to search Create query Lists non-redundant nucleotide sequences from WO Sequence Searching Tools 40
41 SRS: advanced text search Select library tab Select resources to search Create query WO sequences Sequence Searching Tools 41
42 SRS: advanced text search Select library tab WO nucleotide sequence record in NRNL1 Select resources to search Create query WO sequences Sequence Searching Tools 42 Details which other patents also claim this sequence (with NRNL2, would see family grouping)
43 SRS: advanced text search Select library tab Select resources to search Create query NRNL1 sequence record WO sequences Sequence Searching Tools 43
44 SRS: advanced text search Select library tab Select resources to search Create query WO literature WO sequences NRNL1 sequence record Sequence Searching Tools 44
45 SRS: advanced text search EMBL-Bank Find all sequences associated with a patent NRNL1 Find all sequences associated with a patent + identify all patents associated with each sequence NRNL2 Find all sequences associated with a patent + identify all patents in the same family associated with each sequence Sequence Searching Tools 45
46 For more information Non-redundant 46
47 47 For more information User Manual Publication
48 48 Help Contacts:
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