Particle size range (0.8 microns 1000 microns)

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3 Occhio FC00M TECHNICAL DATASHEET Reference code: OCC4-0 Occhio Flowcell FC00 M Particle size range (0.8 microns 000 microns) Technical specifications Working conditions Working temperature 5-40 C non condensing Power Supply 00-0 Vac 50-60Hz Computer (if supplied by Occhio, minimum specifications) Processor Intel Core 4MB cache Ram 4 56MHz Hard Disk 500MB Display LCD, FullHD,.5 Mouse, keyboard USB (English) Operating system Windows Seven compatible with XP, Vista Optics and imaging device Standard camera type C-mos progressive scan Camera resolution 6.6 Millions pixels (00 x 3000 pixels) Pixel size 3.5 µm side Lens type Telecentric variable magnification zoom Lens resolution From 0.38 to.33 µm/pixel Field of view 836 x 40 µm/pixel 533 x 7000 µm/pixel Light source Collimated monochromatic light Light wavelength 440 nm Light output diameter 5 mm FC00M Ref: OCC4-0 (C) Copyright 0 OCCHIO s.a. All rights reserved.

4 Occhio FC00M TECHNICAL DATASHEET Dimensions and weight Length 630 mm 4.8 in Width 350 mm 3.8 in Height 60 mm 6.3 in Weight 7.5 Kg 40.8 lbs Connection USB II at 480Mbps, DIN5Pin 80, DIN3pin Integrated membrane pump Power supply 0-Volt DC The pump is powered by internal power supply. Voltage variables 0 to Vdc via a potentiometer located on the left side of the instrument Valves and membrane Polypropylene and PTFE Pumping flow 0 to 000ml/min FC00M Ref: OCC4-0 (C) Copyright 0 OCCHIO s.a. All rights reserved.

5 Occhio FC00M TECHNICAL DATASHEET Starting kit parts (these parts are included in the packing box at the delivery) Part number OCC0SW CALLISTO EXPERT R Flow cell glass windows for FC00M S S+ - HR R Glass windows O-ring for FC00M-S-S+-HR R R R R6000x50µm R6000x400µm R6000x500µm R6000x800µm R6000x000µm R 4-90-R 4-90-R* Pipe flow in/out, AISI 36; 4x6mm length 80mm USB, m/m.5m, instrument connection cables Tube Tygon Solva 4.8x8mm; m Paper spacer channel width 6000µm thickness 50µm Paper spacer channel width 6000µm thickness 400µm Paper spacer channel width 6000µm thickness 500µm Paper spacer channel width 6000µm thickness 800µm Paper spacer channel width 6000µm thickness 000µm Power supply module; 5--4VDC for FC00M Set: 0 screws for flowcell core Set: complete mounted flowcell core for FC00M R R R Power supply cable North America Power supply cable Europe Desk top computer + LCD, FullHD,.5 + Mouse + Keyboard US USB Keyboard USB (FR) instead of Keyboard US according with customer country Particle size standard Borosilicate glass beads 0µm R R x x x x Quantity R R R 4-04-R R-6000x400µm 4-90-R 4-53-R bottle * This part is installed on the instrument at the delivery (standard configuration) FC00M Ref: OCC4-0 (C) Copyright 0 OCCHIO s.a. All rights reserved. 3

6 Occhio FC00M TECHNICAL DATASHEET Option (Ref: 4): stainless steel conical beaker with overhead stirrer Stainless steel 4mm thickness base includes two sides supports columns. Stainless steel beaker Stainless steel conical beaker 300ml Overhead stirrer Max volume liters Consumption 8w Speed rpm power cables delivered with the stirrer, speed and power supply are independent of the instrument Stainless steel inlet outlet tube One tube 6x4mm diameter and 80mm length, compatible with R Tygon Solva 4.8x8mm Tube support One tube support with blocking screw Recommended flow path for 4 option Support module FC00M Ref: OCC4-0 (C) Copyright 0 OCCHIO s.a. All rights reserved. 4

7 Occhio FC00M TECHNICAL DATASHEET FC00M Ref: OCC4-0 (C) Copyright 0 OCCHIO s.a. All rights reserved. 5

8 Occhio FC00M TECHNICAL DATASHEET Occhio FC00M short instrument overview Instrument calibration A first calibration is imposed using the magnification table according with the camera and lens specifications. FC00M Zoom magnification Front lens magnification Global magnification Instrument calibration µm/pixel Image size (µm) Standard camera 00x3000 pixels x x550 4x x65 3x 6x x 9x x67 A second calibration, according with customer specifications, is done using standard latex beads from µm up to hundreds microns. A calibration table is implemented in the software allows computed distribution values through an automatic size correction. Sample analysis Model Sample dispersion Sample particles size range Sample concentration Sample analysis Standard Operating Procedure includes FC00M Ref: OCC4-0 Occhio Flowcell FC00M Water, alcohols, oil (viscosity depending) From 800nm to mm (FC00M) According with sample property and flowcell thickness (Typical dilution 5% in volume) Size distribution cumulate and proportional curve Number distribution or volume weighted distribution Particles counting distribution (size expressed in particles/ml for each size bins) Analysis volume(priming, analysis, rinsing) Volume sampling Light intensity calibration Background calibration Particles counting (C) Copyright 0 OCCHIO s.a. All rights reserved. 6

9 Occhio FC00M TECHNICAL DATASHEET Creation of a particle database Image storage Filtering procedure Automatic reporting generation Software mains features Model Size parameters (Iso 976-6; 7; 8) All the size parameters are displayable or not according with the customer setting preference Shape parameters (Iso 976-6; 7; 8) All the shape parameters are displayable or not according with the customer setting preference Advanced shape parameters Image format Data storage Data comparisons Plots and figure (By number or volume weighted values) FC00M Ref: OCC4-0 Callisto Software for Flowcell FC00M ISO Area diameter ISO Inner diameter Mean diameter Perimeter diameter Crofton diameter Half Crofton diameter Width Length Ellipse Width Ellipse Length ISO Max Distance ISO Geodesic Length Occhio Bluntness Occhio Roughness Elongation ISO Aspect Ratio Ellipsoid Elongation Ellipsoid Roundness Ellipse Ratio ISO Eccentricity ISO Straightness ISO Roundness ISO Compactness ISO Extent ISO Solidity Convexity ISO Circularity Luminance mean Luminance var. Porosity Developed in function of customer specifications Bitmap.oph binary Occhio files format contains: Full size distribution values Shape and size percentiles Outline and greyscale levels of each particle Open and compare more analysis on the same plots include trends graphic Acquisition info (short overview of the used SOP) Size distribution Size percentiles Shape percentiles (C) Copyright 0 OCCHIO s.a. All rights reserved. 7

10 Occhio FC00M TECHNICAL DATASHEET Statistics tools Reporting and data export Microscope mode pane Shape distribution Mean shape by size D scatter-plot (fully selectable particles map) 3D scatter-plot (include animation) Percentiles sample images Sample images (BMP exportable format) Id card for each particle (BMP exportable format) Morphological and size filtering procedure Raw data export (text format) Table distribution export (text format) Table distribution and percentile export (Excel format) Automatic or custom reporting Full image export (bmp format) Single particle image export (bmp format) Figure and graph export(bmp format) Manual pumping fast speed, low speed. Valve switching, rinsing procedure. Current live image analysis. OCCHIO SA 4 rue des chasseurs ardennais BELGIUM Tel : Fax : info@occhio.be QAQC LAB LLC 589 Rappahannock Drive White Stone Va 578 Tel (804) Fax(703) FC00M Ref: OCC4-0 (C) Copyright 0 OCCHIO s.a. All rights reserved. 8